Gene interactions and pathways from curated databases and text-mining

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IL12A — IL12B

Pathways - manually collected, often from reviews:

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

Text-mined interactions from Literome

Taniguchi et al., Gene Ther 1998 (Fibrosarcoma...) : IL-12 also stimulates IFN-gamma production from both T cells and NK cells
Pardoux et al., Blood 1999 : In addition, TGF-beta was found to significantly inhibit IL-12 induced phosphorylation of the STAT4 transcription factor ... Electrophoretic mobility shift assay indicated that TGF-beta induced a decrease in IL-12 induced STAT4 DNA binding activity in T lymphocytes
Coccia et al., J Biol Chem 1999 : No significant differences of IRF-1 mRNA expression were found in established Th1 and Th2 cells ; however, interleukin 12 (IL-12) induced a strong up-regulation of IRF-1 transcripts in Th1 but not in Th2 cells ... We demonstrate that IL-12 induced up-regulation of IRF-1 is mediated by signal transducer and activator of transcription-4, which binds to the interferon (IFN)-gamma activated sequence present in the promoter of the IRF-1 gene ... Strong IL-12 dependent activation of a reporter gene construct containing the IRF-1 IFN-gamma activated sequence element provides further evidence for the key role of signal transducer and activator of transcription-4 in the IL-12 induced up-regulation of IRF-1 transcripts in T cells
Yang et al., Eur J Immunol 1999 : However, IL-12 and IL-18 together fully induce IFN-gamma transcription independently of TCR activated signals, by a mechanism that does not simply involve Stat4 and NF-kappaB activation, but requires additional protein synthesis ... Cyclosporin A inhibits TCR induced IFN-gamma production, but not IL-12/IL-18 induced IFN-gamma production, biochemically discriminating between these pathways
Matikainen et al., Blood 1999 : IL-12 induced STAT4 and IL-2 and IL-15 induced STAT5 binding to the GAS elements ... IL-12 induced STAT4 and IL-2 and IL-15 induced STAT5 binding to the GAS elements ... IL-12 induced STAT4 and IL-2 and IL-15 induced STAT5 binding to the GAS elements
Avice et al., J Immunol 1999 : Soluble LAG-3Ig fusion protein markedly enhances IL-12 production by monocytes stimulated with infra-optimal concentrations of sCD40L, whereas it directly stimulates monocyte derived dendritic cells ( DC ) for the production of TNF-alpha and IL-12, unravelling an enhanced responsiveness to MHC class II engagemenent in DC as compared with activated monocytes
Sin et al., J Immunol 1999 : IL-12 gene as a DNA vaccine adjuvant in a herpes mouse model : IL-12 enhances Th1-type CD4+ T cell mediated protective immunity against herpes simplex virus-2 challenge ... However, the production of cytokines ( IL-4 and IL-10 ) and chemokine ( MCP-1 ) was inhibited by IL-12 coinjection ... However, the production of cytokines ( IL-4 and IL-10 ) and chemokine ( MCP-1 ) was inhibited by IL-12 coinjection
Sudarshan et al., J Immunol 1999 : We examined this and found that TGF-beta1 did not have any effect on IL-12 induced phosphorylation of JAK2, TYK2, and STAT4 although TGF-beta1 inhibited IL-2- and IL-12 induced IFN-gamma production ... We examined this and found that TGF-beta1 did not have any effect on IL-12 induced phosphorylation of JAK2, TYK2 , and STAT4 although TGF-beta1 inhibited IL-2- and IL-12 induced IFN-gamma production ... We examined this and found that TGF-beta1 did not have any effect on IL-12 induced phosphorylation of JAK2, TYK2, and STAT4 although TGF-beta1 inhibited IL-2- and IL-12 induced IFN-gamma production ... We examined this and found that TGF-beta1 did not have any effect on IL-12 induced phosphorylation of JAK2 , TYK2, and STAT4 although TGF-beta1 inhibited IL-2- and IL-12 induced IFN-gamma production
Chang et al., J Exp Med 1999 (Encephalomyelitis, Autoimmune, Experimental) : Defective expression was not secondary to the production of suppressive cytokines, but to a failure of B10.S MBP-specific T cells to upregulate CD40 ligand expression and to induce the production of IL-12
Lim et al., J Immunol 1999 : IL-12 induces P-selectin ligand, while IL-4 plays a dominant role in down regulating E-selectin ligand
Chen et al., J Immunol 1999 : Finally, human hsp60 induces gene expression of the Th1 promoting cytokines IL-12 and IL-15
Kaliński et al., J Immunol 1999 : A reduced IL-12 producing capacity of mature DC resulted mainly from their impaired responsiveness to IFN-gamma, a cofactor in CD40 induced IL-12 p70 production
Curtsinger et al., J Immunol 1999 (Inflammation) : IL-1, but not IL-12 , increased proliferation of CD4+ T cells in response to Ag and IL-2 , which is consistent with effects on in vivo priming of CD4+ cells
Kitamura et al., J Exp Med 1999 : Production of IFN-gamma by NKT cells in response to alpha-GalCer required IL-12 produced by dendritic cells (DCs) and direct contact between NKT cells and DCs through CD40/CD40 ligand interactions
Murosaki et al., Biosci Biotechnol Biochem 1999 (Escherichia coli Infections) : NOS enhanced interleukin 12 (IL-12) and interferon-gamma (IFN-gamma) production by normal splenocytes in the presence of the potent IL-12 inducer, heat killed Lactobacillus plantarum L-137, in vitro
Wang et al., J Clin Invest 1999 (Mycobacterium Infections) : Endogenous IL-18 ( IFN-gamma inducing factor ) played little role in IFN-gamma responses by macrophages stimulated by mycobacteria and IL-12 ... Our data reveal that macrophages are a significant source of type 1 cytokines during mycobacterial infection and that both IL-12 and intracellular pathogens are required for the release of IFN-gamma but not TNF-alpha ... Our data reveal that macrophages are a significant source of type 1 cytokines during mycobacterial infection and that both IL-12 and intracellular pathogens are required for the release of IFN-gamma but not TNF-alpha ... These findings suggest that macrophages regulate cell mediated immunity by releasing not only IL-12 and TNF-alpha but also IFN-gamma and that full activation of IFN-gamma response in macrophages is tightly regulated
Rogge et al., J Immunol 1999 (Asthma...) : In the presence of IL-12 , cell surface expression of the IL-12R beta 2 subunit was readily detected on T cells after 24 h, reached the maximum at day 5, and declined thereafter
Poggi et al., J Immunol 1999 (Multiple Sclerosis) : IL-12 mediated NKRP1A up-regulation and consequent enhancement of endothelial transmigration of V delta 2+ TCR gamma delta+ T lymphocytes from healthy donors and multiple sclerosis patients ... No up-regulation of NKRP1A by IL-12 was noted on V delta 1+ clones ... We conclude that the expression of NKRP1A on V delta 2+ cells is associated with increased ability to migrate across the vascular endothelium and that this phenomenon may be regulated by IL-12 present in the microenvironment
Gollob et al., J Immunol 1999 : A specific inhibitor of p38 MAP kinase completely inhibits the serine phosphorylation of STAT1 and STAT3 induced by IL-12 and IL-2 and abrogates the functional synergy between IL-12 and IL-2 without affecting STAT tyrosine phosphorylation ... A specific inhibitor of p38 MAP kinase completely inhibits the serine phosphorylation of STAT1 and STAT3 induced by IL-12 and IL-2 and abrogates the functional synergy between IL-12 and IL-2 without affecting STAT tyrosine phosphorylation
Cousens et al., J Exp Med 1999 (Lymphocytic Choriomeningitis) : Although interleukin (IL)-12 has the potential to contribute, IL-12 dependent T cell IFN-gamma has not been detected during viral infections
Carter et al., J Exp Med 1999 : Both lineages require signal transducer and activator of transcription ( Stat ) 4 activation for IFN-gamma induced by interleukin (IL)-12/IL-18 signaling, but only CD4 ( + ) T cells require Stat4 for IFN-gamma induction via the TCR pathway ... In response to antigen, CD8 ( + ) T cells can produce IFN-gamma independently of IL-12, whereas CD4 ( + ) T cells require IL-12 and Stat4 activation
Flieger et al., Hybridoma 1999 (Colorectal Neoplasms) : By contrast, IL-4 reduced the IL-2, IL-12 , and IFN-alpha induced ADCC
Prinz et al., J Neurochem 1999 : IL-18 inhibits the LPS induced release of IL-12 and attenuates that of TNF-alpha, whereas the production of IL-6 and macrophage inflammatory protein-1alpha is only marginally affected ... IL-18 inhibits the LPS induced release of IL-12 and attenuates that of TNF-alpha, whereas the production of IL-6 and macrophage inflammatory protein-1alpha is only marginally affected
Delespesse et al., Int Arch Allergy Immunol 1999 : The production of IL-4 and IL-13 by naive T cells is differentially regulated by TGF-beta and IL-12 ... The production of IL-4 and IL-13 by naive T cells is differentially regulated by TGF-beta and IL-12
Hoshino et al., J Immunol 1999 : In contrast, IL-12 did not enhance the IL-13 production induced by IL-2 alone ... In contrast, IL-10 production induced by IL-2 and/or IL-12 was not significantly different in IFN-gamma ( -/- ) mice and normal controls
Levings et al., J Immunol 1999 : IL-4 inhibits the production of TNF-alpha and IL-12 by STAT6 dependent and -independent mechanisms ... We report here that the inhibitory effects of IL-4 on the production of TNF-alpha or IL-12 by macrophages had both STAT6 dependent and -independent components, depending on the stimuli ... However, IL-4 still induced significant inhibition of the production of TNF-alpha and IL-12 from STAT6 null macrophages that were stimulated with the more physiologically relevant combination of LPS and IFN-gamma ... These data show that STAT6 is required for the IL-4 mediated inhibition of the production of TNF-alpha and IL-12 stimulated by LPS alone, but that IL-4 also activates distinct, STAT6 independent mechanism(s) that inhibit the IFN-gamma mediated enhancement of IL-12 and TNF-alpha production ... These data show that STAT6 is required for the IL-4 mediated inhibition of the production of TNF-alpha and IL-12 stimulated by LPS alone, but that IL-4 also activates distinct, STAT6 independent mechanism(s) that inhibit the IFN-gamma mediated enhancement of IL-12 and TNF-alpha production ... These data show that STAT6 is required for the IL-4 mediated inhibition of the production of TNF-alpha and IL-12 stimulated by LPS alone, but that IL-4 also activates distinct, STAT6 independent mechanism(s) that inhibit the IFN-gamma mediated enhancement of IL-12 and TNF-alpha production
Walker et al., J Immunol 1999 : IL-18 and CD28 use distinct molecular mechanisms to enhance NK cell production of IL-12 induced IFN-gamma
García et al., J Immunol 1999 (Leprosy...) : Anti-IL-12 partially inhibited M. leprae induced release of IFN-gamma in the presence of IL-18, suggesting a combined effect of IL-12 and IL-18 in promoting M. leprae-specific type 1 responses
Bright et al., J Immunol 1999 (Encephalomyelitis, Autoimmune, Experimental) : Tyrphostin B42 inhibits IL-12 induced tyrosine phosphorylation and activation of Janus kinase-2 and prevents experimental allergic encephalomyelitis ... Treatment of activated T cells with tyrphostin B42 inhibited the IL-12 induced tyrosine phosphorylation and activation of Jak-2 without affecting Tyk-2 kinase ... Inhibition of either Jak-2 or Tyk-2 leads to a decrease in the IL-12 induced tyrosine phosphorylation of Stat3 , but not of Stat4, protein ... Inhibition of either Jak-2 or Tyk-2 leads to a decrease in the IL-12 induced tyrosine phosphorylation of Stat3, but not of Stat4 , protein
Kato et al., Int J Immunopharmacol 1999 : Thus, we confirmed that IFN-gamma production by splenocytes was the result of the production of IL-12 from X-irr-Spl stimulated by LcS ... The IL-12 induced by LcS stimulated the production of IFN-gamma
Bonder et al., Immunology 1999 (Arthritis, Rheumatoid) : While IL-4 suppressed lipopolysaccharide (LPS) induced IL-12 and IL-10 production by human peripheral blood monocytes, IL-4 suppressed LPS induced IL-12 , but not IL-10, production by synovial fluid mononuclear cells from patients with rheumatoid arthritis ... While IL-4 suppressed lipopolysaccharide (LPS) induced IL-12 and IL-10 production by human peripheral blood monocytes, IL-4 suppressed LPS induced IL-12, but not IL-10, production by synovial fluid mononuclear cells from patients with rheumatoid arthritis ... IL-4 also suppressed IL-12 , but not IL-10 production, by LPS stimulated in vitro monocyte derived macrophages
Noma et al., J Allergy Clin Immunol 1999 (Asthma) : IL-12 affects Dermatophagoides farinae induced IL-4 production by T cells from pediatric patients with mite-sensitive asthma ... We studied the effect of IL-12 on Dermatophagoides farinae ( Df ) antigen induced IL-4 production and subsequent production of IgE by PBMCs from pediatric patients with asthma
Sutterwala et al., J Leukoc Biol 1999 : IL-12, and the interferon-gamma (IFN-gamma) that is induced by IL-12 , play central roles in the development of the Th1-type immune responses that are required for immunity to intracellular pathogens
Bright et al., J Leukoc Biol 1999 : On binding to IA ( S ), SEB induces IL-12 production in macrophages, which in turn augments antigen induced proliferation of HS-17 T cells
Fox et al., J Interferon Cytokine Res 1999 (Sezary Syndrome) : Furthermore, IL-12 in vitro can restore IFN-gamma production by these patients ' cells to near normal levels ... The IFN-y inducing effect of ATRA and 13-cis-retinoic acid could be abrogated by addition of anti-IL-12 antibodies, suggesting that IL-12 plays a role in the synergistic upregulation of IFN-gamma
Kikuchi et al., J Immunother 1999 (Brain Neoplasms...) : The concentration of IL-10 induced by IL-12 increased in the supernatant of human PBMCs costimulated with IL-2 and IL-12
Delgado et al., J Neuroimmunol 1999 (Endotoxemia) : VIP and PACAP inhibit IL-12 production in LPS stimulated macrophages ... VIP and PACAP inhibit IL-12 production in LPS stimulated macrophages ... This study examines the effect of two neuropeptides , vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase activating polypeptide ( PACAP ), on interleukin-12 (IL-12) production ... Type 1 VIP receptor ( VPAC1 ), and to a lesser degree type 2 VIP receptor ( VPAC2 ), mediate the inhibition of IL-12 , primarily through the cAMP/PKA pathway ... VIP/PACAP inhibit the production of IL-12 , IL-6, tumor necrosis factor alpha (TNFalpha), and interferon gamma (IFNgamma) in vivo in endotoxemic mice ... VIP/PACAP inhibit the production of IL-12 , IL-6, tumor necrosis factor alpha (TNFalpha), and interferon gamma (IFNgamma) in vivo in endotoxemic mice
Yogi et al., Int J Lepr Other Mycobact Dis 1998 (Disease Susceptibility...) : To examine the reason why IFN-gamma can not be produced by splenocytes of ALY mice inoculated with M. leprae, we detected cytokine gene expression and IFN-gamma induction in the presence of recombinant murine IL-12 or IGIF/IL-18
Corinti et al., J Immunol 1999 : In parallel, CD43 cross linking induced synthesis and release of IL-1beta, IL-6, TNF-alpha, IL-12 , and IL-10
Yamane et al., J Immunol 1999 : These results indicate that the p35 mRNA accumulation induced by MHC class II stimulation plays a role in bioactive IL-12 production
Subauste et al., J Immunol 1999 (Chronic Disease...) : Not only was IL-12 production in response to T. gondii dependent on CD40-CD40L signaling, but also, patients with HIGM syndrome exhibited deficient in vitro secretion of this cytokine in response to the parasite ... Not only was IL-12 production in response to T. gondii dependent on CD40-CD40L signaling, but also, patients with HIGM syndrome exhibited deficient in vitro secretion of this cytokine in response to the parasite
Wittmann et al., J Immunol 1999 : The results of several different experimental approaches suggest that IL-12 down-regulation was not due to endogenous IL-10 , IL-4, or PGE2 production induced by C5a ... The results of several different experimental approaches suggest that IL-12 down-regulation was not due to endogenous IL-10, IL-4 , or PGE2 production induced by C5a
Mullins et al., J Immunol 1999 (Sarcoma, Experimental) : Because paclitaxel demonstrates LPS-mimetic capabilities and increased production of the LPS induced immunostimulatory cytokine IL-12 could account for enhanced T cell responsiveness, we investigated whether paclitaxel induces Mphi IL-12 production ... In contrast, paclitaxel significantly enhanced both normal host and TBH Mphi IL-12 p70 production in vitro, although TBH Mphi IL-12 production was lower than that of similarly treated normal host Mphis
Arnold et al., J Immunol 1999 : Stimulation of the cells with IL-6, IL-10, IL-12 , TGF-beta1, and GM-CSF did not modulate the constitutive FasL expression, but IFN-gamma mediated FasL up-regulation was significantly diminished by IL-10 and TGF-beta1, respectively
Kobayashi et al., J Immunol 1999 (Burns...) : Although IL-12 did not stimulate IFN-gamma production in cultures of splenic T cells from TI mice, IFN-gamma was produced by stimulation with IL-12 when the producer cells were prepared from TI mice that had been treated previously with sIL-4R
Galon et al., J Immunol 1999 : IL-12 induces IFN regulating factor-1 (IRF-1) gene expression in human NK and T cells ... Furthermore, IL-2 and IL-12 synergistically induced IRF-1 , whereas IFN-alpha and IL-12 did not ... In contrast to IL-12, no up-regulation of IRF-1 was found in IL-4 stimulated cells, and IL-4 did not block IL-12 dependent up-regulation of IRF-1
Yue et al., Eur J Immunol 1999 (Melanoma) : Preincubation with IL-12 increased the surface levels of human leukocyte antigen ( HLA ) class I, HLA class II and intercellular adhesion molecule (ICAM)-1 of those cultures with IL-12Rbeta expression
Grdic et al., Eur J Immunol 1999 : CT had no inhibitory effects on lipopolysaccharide/IFN-gamma induced IL-12 mRNA expression or IL-12 production
Jung et al., Clin Exp Allergy 1999 (Dermatitis, Atopic) : IL-12 present at priming or high doses of IL-2 during the culture period, even in the absence of IL-12, completely restored IFN-gamma production
Tomura et al., J Immunol 1999 : Production of IL-12 preceded IFN-gamma production, and IL-12 was required for IFN-gamma, but not IL-4, production ... Production of IL-12 preceded IFN-gamma production, and IL-12 was required for IFN-gamma, but not IL-4 , production
Bartholomé et al., J Interferon Cytokine Res 1999 : We conclude that the direct action of IFN-beta on DC causes inhibition of their ability to secrete IL-12 in response to CD40 ligation and to elicit Th1 type responses
Shrayer et al., Clin Exp Metastasis 1999 (Lung Neoplasms...) : Interleukin (IL)-12 can activate cytotoxic lymphocytes, stimulate natural killer cell activity, induce the production of INF-gamma and inhibit the development of various experimental tumors
Rakhit et al., Clin Pharmacol Ther 1999 (Carcinoma, Renal Cell...) : Adverse events, serum IL-12 levels, and serum levels of interferon-gamma (IFN-gamma) and interleukin-10 (IL-10) produced in response to IL- 12 were all maximum in the week after the first dose of rHuIL-12 and decreased after long-term administration ... Adverse events, serum IL-12 levels, and serum levels of interferon-gamma (IFN-gamma) and interleukin-10 (IL-10) produced in response to IL- 12 were all maximum in the week after the first dose of rHuIL-12 and decreased after long-term administration ... The down-regulation of serum IFN-gamma levels correlated with decreased IFN-gamma messenger ribonucleic acid expression and may result from feedback inhibition of IL-12 signaling or from a more specific inhibition of IFN-gamma synthesis
Harley et al., Clin Diagn Lab Immunol 1999 (Chronic Disease...) : Expression of IL-2, IL-10, IL-12 ( p35 and p40 ), and IFN-gamma was detected in most nondiseased biopsies, while IL-6 was detected in a minority, and IL-4 and IL-5 were both undetectable
Zhang et al., J Immunol 1999 : Reporter transgenic mice expressing the luciferase gene under the control of separate TCR-response elements ( TCR-RE ) from the IFN-gamma promoter or expressing the green fluorescent protein gene under the control of an IFN-gamma `` minigene '' were employed to explore the basis for IL-12 regulation of IFN-gamma gene transcription
Karp et al., Immunol Rev 1999 (Measles) : CD46 mediated downregulation of IL-12 has turned out to be a specific instance of a more general pattern of tight inhibitory control over IL-12 production effected by complement and phagocytic receptors on antigen presenting cells
Méndez-Samperio et al., Scand J Immunol 1999 : In this study, we investigated the capacity of the Bacille Calmette-Guérin ( BCG ) strain of Mycobacterium bovis to induce the production of interferon-gamma through IL-12 and/or CD40 costimulation from human cells ... Our data demonstrate that anti-IL-12 antibodies markedly reduced the levels of IFN-gamma produced by the BCG stimulated human cells, while exogenous recombinant ( r ) IL-12 up-regulated the production of IFN-gamma ... In addition, the stimulatory effect of IL-12 on BCG induced IFN-gamma secretion was specific, as it was significantly abolished in the presence of anti-IL-12 antibodies ... Furthermore, the presence of a trimeric soluble CD40L agonist (CD40T) in cultures increased the production of IL-12 and IFN-gamma ... Together, these studies are the first to demonstrate that BCG induced IFN-gamma production by human cells appears to be mediated by IL-12 in a CD40 dependent manner and suggest that CD40-CD40L activation may be a critical mediator in regulating the immune response to stimulation with BCG ... Together, these studies are the first to demonstrate that BCG induced IFN-gamma production by human cells appears to be mediated by IL-12 in a CD40 dependent manner and suggest that CD40-CD40L activation may be a critical mediator in regulating the immune response to stimulation with BCG
Micallef et al., Cancer Immunol Immunother 1999 : IL-18 and IL-12 synergistically augment IFNgamma production reportedly because IL-12 enhances IL-18 receptor (IL-18R) expression ... IL-18 and IL-12 synergistically augment IFNgamma production reportedly because IL-12 enhances IL-18 receptor (IL-18R) expression
Cutolo et al., Ann N Y Acad Sci 1999 (Arthritis, Rheumatoid) : Our results therefore show that MLT induces IL-12 secretion and NO production by unstimulated cultured RA synovial macrophages and human monocytic myeloid THP-1 cells
Moore et al., Vaccine 1999 : 3D-MPL was found to enhance B7-1 expression and IL-12 production by macrophages, which are known to be involved in the LPS induced enhancement of Th1 responses
Butler et al., Eur J Immunol 1999 (Arthritis) : When the antibodies were added to synovial cells from arthritic mice and bone marrow macrophages in vitro, anti-TNF diminished IL-12 production, but anti-IL-12 had no effect on TNF production
Peterson et al., Eur J Immunol 1999 : IL-2 stimulated SLP-76 ( -/- ) splenocytes also retain normal antibody dependent cellular cytotoxicity and the ability to secrete IFN-gamma in response to IL-12 stimulation
Piccinni et al., Eur J Immunol 1999 : The promoting effect of relaxin on the development of IFN-gamma producing cells was not due to a relaxin induced release of IL-12 and/or IFN-alpha by antigen presenting cells
Saha et al., Eur J Immunol 1999 (Leishmaniasis, Cutaneous) : LPS induced IL-12 and TNF-alpha secretion by both the susceptible and resistant strain derived macrophage subsets are down-regulated
Szekeres-Bartho et al., Am J Reprod Immunol 1999 : Signaling via the V gamma 1.4V delta 1 receptor induces a Th2 type response, whereas activation of the lymphocytes via the V gamma 9V delta 2 receptor results in increased IL-12 production and natural killer ( NK ) activity
Larsson et al., Br J Pharmacol 1999 : IL-2 and IL-4 counteract budesonide inhibition of GM-CSF and IL-10, but not of IL-8, IL-12 or TNF-alpha production by human mononuclear blood cells
Collins et al., Vet Immunol Immunopathol 1999 : IL-12 did not affect the proliferative responses of PBMC to these antigens but markedly accelerated and augmented the level of IFNgamma secreted ... The effect of IL-12 on IFNgamma synthesis was confirmed at the level of IFNgamma
Bliss et al., J Immunol 1999 (Agranulocytosis...) : Production of IL-12 does not require TNFR p55 , and release of TNF-alpha occurs independently of IL-12
Cheung et al., Mol Immunol 1999 : Thus ligation of CD28 in the presence of IL-12 results in a synergistic increase in production of the cytokine
Vanham et al., Clin Exp Immunol 1999 (Acquired Immunodeficiency Syndrome...) : Stimulation of mononuclear cells through cell bound CD40L and soluble IFN-gamma induced significantly higher IL-12 in cultures from patients with > 200 circulating CD4 T cells, whereas IL-12 production was marginally decreased in cultures from patients with < 200 CD4 T cells, compared with healthy control cultures ... Stimulation of mononuclear cells through cell bound CD40L and soluble IFN-gamma induced significantly higher IL-12 in cultures from patients with > 200 circulating CD4 T cells, whereas IL-12 production was marginally decreased in cultures from patients with < 200 CD4 T cells, compared with healthy control cultures
Hu et al., Immunology 1999 : In the present work we analysed the effects of IL-12 on the synthesis and secretion of IFN-gamma and IL-4 by human T-cell clones ... In the present work we analysed the effects of IL-12 on the synthesis and secretion of IFN-gamma and IL-4 by human T-cell clones ... We demonstrated, by enzyme linked immunosorbent assay ( ELISA ) and intracytoplasmic staining, that, in Th0 clones, IL-12 up-regulated the production of both IFN-gamma and IL-4 and was unable to modulate these cells to Th1-type ... We demonstrated, by enzyme linked immunosorbent assay ( ELISA ) and intracytoplasmic staining, that, in Th0 clones, IL-12 up-regulated the production of both IFN-gamma and IL-4 and was unable to modulate these cells to Th1-type ... In Th1 cells, IL-12 up-regulated only IFN-gamma and not IL-4 ... However, in Th2 cells, both IFN-gamma and IL-4 were up-regulated by IL-12 ... However, in Th2 cells, both IFN-gamma and IL-4 were up-regulated by IL-12
Bianchi et al., J Immunol 1999 : Ligation of CD40 on dendritic cells ( DC ) triggers production of IL-12 ... We also explore the possibility that the IL-12 resulting from CD40 ligation directly affects the APC function of DC, mediating or contributing to the adjuvant effect of CD40 ligation
Tuo et al., J Interferon Cytokine Res 1999 : Comparative effects of interleukin-12 and interleukin-4 on cytokine responses by antigen stimulated memory CD4+ T cells of cattle : IL-12 enhances IFN-gamma production, whereas IL-4 has marginal effects on cytokine expression ... IL-12 stimulated enhanced levels of IFN-gamma protein and transcript expression in all cell lines, with no consistent effects on IL-2 or IL-4 expression ... In two B. bovis-specific cell lines, IL-12 suppressed IL-10 expression
Roy et al., Surgery 1999 : We harvested adherent peritoneal macrophages harvested from wild-type ( WT ), heterozygous c-fos knockout ( Hetero KO ), or homozygous c-fos knockout ( Homo KO ) mice and investigated lipopolysaccharide (LPS) induced IL-12 p70 protein synthesis ( by enzyme linked immunosorbent assay ), IL-12 p35 and IL-12 p40 messenger RNA accumulation ( mRNA ) ( by reverse transcriptase-polymerase chain reaction ), and the transcription rate ( by nuclear runoff ) ... ( 2 ) LPS induces a significant increase in IL-12 p70 protein, IL-12 p40 mRNA, and the transcription rate in the Homo KO group compared with either the Hetero KO or WT groups ... These data suggest that deficiency of the transcription factor c-fos increases LPS induced macrophage IL-12 production, possibly by simulating the effect of interferon gamma priming
Mountford et al., Immunology 1999 (Granuloma...) : Specifically, mice deficient for the interferon-gamma receptor ( IFN-gammaR-/- ) were used to determine the role for IL-12 in the absence of IFN-gamma mediated signalling ... We show that recombinant IL-12 administered to IFN-gammaR-/- mice caused the up-regulation of mRNA for IFN-gamma in lung tissue, and the secretion of abundant IFN-gamma by in vitro cultured lymph node cells in response to egg antigens
Stamm et al., Eur J Immunol 1999 (Leishmaniasis, Cutaneous) : These results indicate that STAT4 mediated IL-12 signaling is critical for the development of protective Th1 response following L. major infection in genetically resistant mice
Biron et al., Curr Opin Microbiol 1999 (Virus Diseases) : In particular, IFN-alpha/beta acts to inhibit interleukin-12 (IL-12) expression and IL-12 activation of innate natural killer ( NK ) cell IFN-alpha production, while inducing NK cell cytotoxicity and proliferation, and promoting adaptive T cell IFN-alpha responses
Monteleone et al., Clin Exp Immunol 1999 : Interferon-gamma (IFN-gamma) and prostaglandin E2 ( PGE2 ) regulate differently IL-12 production in human intestinal lamina propria mononuclear cells ( LPMC ) ... The aim of this study was to investigate the effect of IFN-gamma and PGE2 on lipopolysaccharide (LPS) stimulated LPMC IL-12 production ... The aim of this study was to investigate the effect of IFN-gamma and PGE2 on lipopolysaccharide (LPS) stimulated LPMC IL-12 production ... To examine the role of endogenous PGE2 on LPS stimulated IL-12 release, LPMC cultures were added of indomethacin before LPS stimulation ... Treatment of LPMC with IFN-gamma facilitated LPS stimulated IL-12 , whereas it completely abrogated IL-10 production ... IL-12 release by LPMC stimulated with IFN-gamma and LPS was significantly inhibited by exogenous IL-10
Akuffo et al., Clin Exp Immunol 1999 : We demonstrate that in an innate response to Leishmania antigen involving NK cells, a critical level of IL-12 is required to induce interferon (IFN)-gamma secretion below which, IL-10 is released in amounts which apparently inhibit IFN-gamma secretion and cellular proliferation
Delneste et al., J Immunol 1999 : Among these neuropeptides, only VIP induces the production of bioactive IL-12 and the neoexpression of CD83 on a fraction of the DC population, with an effect significant at 100 and 10 nM, respectively ... In the presence of a suboptimal concentration of TNF-alpha, which has no detectable effect on DC by itself, VIP induces the production of high levels of bioactive IL-12 , the neoexpression of CD83 on almost all the DC population ( with an effect significant at 10 and 0.1 nM, respectively ), and the up-regulation of various adhesion and costimulatory molecule expression
Grohmann et al., J Immunol 1999 (Hypersensitivity, Delayed) : IL-12 acts selectively on CD8 alpha- dendritic cells to enhance presentation of a tumor peptide in vivo
Villegas et al., J Immunol 1999 (Chronic Disease...) : Analysis of the immune response of acutely infected CD28-/- mice revealed that IL-12 was required for T cell production of IFN-gamma and this was independent of the CD40/CD40 ligand interaction
Schwertschlag et al., Leukemia 1999 (Acute-Phase Reaction) : In addition to effects on macrophages, rhIL-11 also reduces CD4+ T cell production of Th1 cytokines, such as IFN gamma induced by IL-12 , while enhancing Th2 cytokine production
Showe et al., J Immunol 1999 (Sezary Syndrome) : Th2 T cells express only the IL-12R beta 1 and thus do not tyrosine phosphorylate STAT4 in response to IL-12
Fantuzzi et al., J Clin Invest 1999 : IL-12 induced IFN-gamma is dependent on caspase-1 processing of the IL-18 precursor ... In the present study, the role of endogenous IL-18 in the induction of IFN-gamma by IL-12 was investigated in mice ... In the presence of a specific inhibitor of caspase-1 ( also known as IL-1beta converting enzyme, or ICE ) IL-12 induced IFN-gamma from splenocytes was reduced by 85 % ... Using splenocytes from ICE-deficient mice, IL-12 induced IFN-gamma was reduced by 80 % ... Neutralizing anti-IL-18 IgG reduced IL-12 induced IFN-gamma in splenocytes by 85 % ... Extracellular levels of IL-18 significantly correlated with IL-12 induced IFN-gamma and were reduced in cells obtained from ICE-deficient mice ... These data demonstrate that endogenous, ICE cleaved IL-18 significantly contributes to induction of IFN-gamma by IL-12
Delgado et al., J Neuroimmunol 1999 : We reported previously that VIP and PACAP inhibit IL-6, IL-12 , TNF alpha and NO production, and enhance IL-10 production, from lipopolysaccharide (LPS) stimulated macrophages ... We reported previously that VIP and PACAP inhibit IL-6, IL-12 , TNF alpha and NO production, and enhance IL-10 production, from lipopolysaccharide (LPS) stimulated macrophages
Foss et al., Infect Immun 1999 : We found that CT and LPS differentially regulated the expression of interleukin-12 (IL-12) and CD80-CD86 but not that of IL-1beta ... LPS and CT each induced IL-1beta expression in macrophages, while only LPS induced IL-12 and only CT induced CD80-CD86 ... LPS and CT each induced IL-1beta expression in macrophages, while only LPS induced IL-12 and only CT induced CD80-CD86 ... LPS and CT each induced IL-1beta expression in macrophages, while only LPS induced IL-12 and only CT induced CD80-CD86 ... These differences were markedly potentiated in gamma interferon ( IFN-gamma ) -treated macrophages, in which LPS potently induced IL-12 and CD80-CD86 expression
Takayama et al., J Invest Dermatol 1999 : Both GM-CSF and TNF-alpha induced the migration of human Langerhans cells in vitro, whereas IL-1beta, IL-2, IL-10, IL-12 , and IFN-gamma had no effect on Langerhans cell migration ... Both GM-CSF and TNF-alpha induced the migration of human Langerhans cells in vitro, whereas IL-1beta, IL-2, IL-10, IL-12 , and IFN-gamma had no effect on Langerhans cell migration
Osaki et al., Gene Ther 1999 (Fibrosarcoma...) : IL-18 receptors are induced on T cells and NK cells by IL-12 and together they enhance a cellular immune response
Stöckl et al., J Clin Invest 1999 : Finally, monocytes pretreated with HRV-14 were greatly inhibited in their production of IL-12 upon stimulation with IFN-gamma/LPS ... Finally, monocytes pretreated with HRV-14 were greatly inhibited in their production of IL-12 upon stimulation with IFN-gamma/LPS
Lamousé-Smith et al., J Immunol 1999 : In this study, we have investigated in detail the cytokine requirements for the generation of allospecific CD8+ CTL in vitro and have found that IL-4 can augment the generation of anti-CD8-sensitive but not anti-CD8-resistant CTL, whereas IL-2 or IL-12 can augment the generation of both anti-CD8-sensitive and anti-CD8-resistant CTL
O'Donnell et al., J Immunol 1999 : Experiments subtracting endogenous BCG-driven IL-12 using neutralizing Ab or augmenting its activity with supplemental rIL-12 revealed not only that IL-12 plays a dominant role in IFN-gamma induction but also that it is normally dose limiting
Kitagawa et al., Arthritis Rheum 1999 (Arthritis, Rheumatoid) : To investigate the roles of tumor necrosis factor alpha(TNFalpha) and the CD40-CD154 interaction in interleukin-12 (IL-12) production by rheumatoid synovial cells ( SC ) ... To investigate the roles of tumor necrosis factor alpha(TNFalpha) and the CD40-CD154 interaction in interleukin-12 (IL-12) production by rheumatoid synovial cells ( SC ) ... To investigate the roles of tumor necrosis factor alpha(TNFalpha) and the CD40-CD154 interaction in interleukin-12 (IL-12) production by rheumatoid synovial cells ( SC ) ... Effects of anti-CD154 and anti-TNFalpha antibody on spontaneous and lipopolysaccharide (LPS) stimulated IL-12 production by SC were examined ... Effects of anti-CD154 and anti-TNFalpha antibody on spontaneous and lipopolysaccharide (LPS) stimulated IL-12 production by SC were examined ... IL-12 p40 production was also increased by LPS stimulation ... LPS stimulated IL-12 production was inhibited by anti-TNFalpha antibody, but not by T cell depletion and anti-CD154 antibody treatment ... The TNFalpha inhibitor rolipram inhibited LPS stimulated IL-12 p40 production by RA SC more strongly than spontaneous production ... TNFalpha restored LPS stimulated IL-12 production that had been inhibited by rolipram ... TNFalpha restored LPS stimulated IL-12 production that had been inhibited by rolipram
Armant et al., J Exp Med 1999 : We report here that engagement of CD47 by either monoclonal antibody, its natural ligand thrombospondin ( TSP ), or 4N1K ( a peptide of the COOH-terminal domain of TSP selectively binding CD47 ) inhibits IL-12 release by monocytes ... CD47 ligation did not alter transforming growth factor ( TGF ) -beta and IL-10 production, and the suppressive effect on IL-12 was not due to autocrine secretion of TGF-beta or IL-10
Liu et al., Cytokine 1999 (Lupus Erythematosus, Systemic) : Exogenous IL-12 or IFN-gamma significantly inhibited IL-10 gene expression, without affecting IL-6 mRNA or other proinflammatory cytokine mRNA levels
Sykes et al., Biol Blood Marrow Transplant 1999 (Graft vs Host Disease) : These protective effects are mediated by interferon (IFN)-gamma , whose early secretion is induced by IL-12 treatment
Gagro et al., Eur J Immunol 1999 : IL-10 was found to dampen IL-12 actions by suppressing IL-12 dependent IFN-gamma production but failed to perturb the effects of exogenous IFN-gamma
Delgado et al., J Biol Chem 1999 : We showed previously that VIP and PACAP inhibit the production of macrophage derived tumor necrosis factor-alpha, interleukin (IL)-6, nitric oxide, and IL-12 ... We showed previously that VIP and PACAP inhibit the production of macrophage derived tumor necrosis factor-alpha, interleukin (IL)-6, nitric oxide, and IL-12 ... VIP and PACAP inhibit IL-12 ( p40 ) gene expression by affecting both NF-kappaB binding and the composition of the Ets-2 binding complex ... VIP and PACAP inhibit IL-12 ( p40 ) gene expression by affecting both NF-kappaB binding and the composition of the Ets-2 binding complex ... VIP and PACAP inhibit IL-12 ( p40 ) gene expression by affecting both NF-kappaB binding and the composition of the Ets-2 binding complex
Hartung et al., Clin Pharmacol Ther 1999 : IL-12 added in vitro to lipopolysaccharide stimulated blood of filgrastim treated donors restored IFN-gamma and TNF-alpha release, suggesting that the anti-inflammatory effect of granulocyte colony stimulating factor is exercised through IL-12 suppression ... IL-12 added in vitro to lipopolysaccharide stimulated blood of filgrastim treated donors restored IFN-gamma and TNF-alpha release, suggesting that the anti-inflammatory effect of granulocyte colony stimulating factor is exercised through IL-12 suppression
Pisetsky et al., J Interferon Cytokine Res 1999 : The IFN-gamma production was nevertheless dependent on IL-12 , as shown by the effects of anti-IL-12 antibodies
Thibodeaux et al., J Immunol 1999 : Autocrine regulation of IL-12 receptor expression is independent of secondary IFN-gamma secretion and not restricted to T and NK cells
Lauw et al., J Infect Dis 1999 (Melioidosis) : Production of IFN-gamma is stimulated by synergistic effects of interleukin (IL)-18, IL-12 , and IL-15 ... In whole blood stimulated with heat killed B. pseudomallei, anti-IL-12 had a stronger inhibitory effect than anti-IL-18 and anti-IL-15 on IFN-gamma production ... These data suggest that during gram negative sepsis, IFN-gamma production is controlled at least in part by endogenous IL-18, IL-12 , and IL-15
Sinigaglia et al., Immunol Rev 1999 (Disease Models, Animal) : IL-12 signaling results in STAT4 activation and interferon (IFN)-gamma production ... IL-12 signaling results in STAT4 activation and interferon (IFN)-gamma production
Kang et al., Immunol Lett 1999 : TPCK and TLCK potently inhibited this LPS induced IL-12 production in a dose dependent manner ... Our results show that TPCK and TLCK inhibit NFkappaB mediated IL-12 production in macrophages
Dybing et al., Infect Immun 1999 (Salmonella Infections, Animal) : As such, several different cytokines including, interleukin-12 (IL-12) and IL-18, can induce IFN-gamma
Huang et al., Infect Immun 1999 : In the absence of IL-10, B. abortus LPS induced strong IFN-gamma responses, but IL-12 p70 levels were still undetectable from BALB/c spleen cells ... In the absence of IL-10, B. abortus LPS induced strong IFN-gamma responses, but IL-12 p70 levels were still undetectable from BALB/c spleen cells ... LPS induced IL-12 if the spleen cells were primed with IFN-gamma and IL-10 was neutralized, indicating that LPS can stimulate IL-12 production under the most favorable conditions
Aicher et al., J Immunol 1999 : SB203580 strongly blocked CD40 induced IL-12 p40 production in DCs at both mRNA and protein levels, while having minimal effect on CD40 induced expression of the chemokine RANTES
Yang et al., Parasite Immunol 1999 (Granuloma) : IL-12 activity was detected in the culture supernatant of T clones stimulated with SEA in the presence of APC ( antigen presenting cells )
Feng et al., J Immunol 1999 : In contrast, while p38 promotes induction of IL-12 ( p40 ) mRNA, ERK activation suppresses LPS mediated IL-12 transcription ... In contrast, while p38 promotes induction of IL-12 ( p40 ) mRNA, ERK activation suppresses LPS mediated IL-12 transcription ... Extracellular signal related kinase ( ERK ) and p38 mitogen activated protein ( MAP ) kinases differentially regulate the lipopolysaccharide mediated induction of inducible nitric oxide synthase and IL-12 in macrophages : Leishmania phosphoglycans subvert macrophage IL-12 production by targeting ERK MAP kinase
Hafner et al., Eur Cytokine Netw 1999 (Fibrosarcoma...) : While the IL-12 induced antimetastatic effect of NK cells was dependent on IFN-gamma action, IL-12 activation of alphabetaNKT cells did not involve IFN-gamma
Kaisho et al., J Exp Med 1999 : In addition, the ability of C/EBPgamma ( -/- ) chimera splenocytes to produce interferon (IFN)-gamma in response to IL-12 and/or IL-18 was markedly impaired
Van Der Vliet et al., Immunology 1999 : Although IL-7 resulted in a decrease in GrB levels in KRN7000 cultured Valpha24+ Vbeta11+ T cells, IL-12 increased GrB levels in both Valpha24+ Vbeta11+ T cells and in Valpha24+ Vbeta11+ T-cell clones and increased cytotoxicity against hCD1d transfected HeLa cells
Chang et al., J Immunol 2000 (Encephalomyelitis, Autoimmune, Experimental) : We demonstrate that IL-2 and IL-12 resulting from CD28/B7 interactions both play a critical role in the induction of expression of the IL-12Rbeta2 subunit and as a result the differentiation of pathogenic autoreactive effector cells
Haicheur et al., Clin Exp Immunol 2000 (Neoplasms...) : In contrast, in most patients IL-12 increased IL-10 expression with no subsequent decrease during the course of therapy, and even an earlier peak of IL-10 induction at the 6th cycle
Lalmanach et al., Microbes Infect 1999 (Genetic Predisposition to Disease...) : IL-12 produced by macrophages is an inducer of IFN-gamma production, which in turn activates the macrophage antibacterial activity and synergizes its effects with TNF-alpha
Hino et al., Cytokine 2000 : However, the stimulation only with LPS induced IL-12 production of spleen cells preincubated in the absence of IFN-gamma ... In the presence of anti-IL-10 , f-spleen cells produced IL-12 upon LPS stimulation, indicating that the failure of f-spleen cells in IL-12 production is caused by IL-10 produced by themselves upon LPS stimulation ... In addition, f-spleen cells produced IL-12 upon CD40 ligand stimulation, and the production was hardly affected by the presence of IFN-gamma or preincubation
Sica et al., J Immunol 2000 (Fibrosarcoma...) : Autocrine production of IL-10 mediates defective IL-12 production and NF-kappa B activation in tumor associated macrophages
Pien et al., J Immunol 2000 (Lymphocytic Choriomeningitis) : Some, but not all, viral infections induce endogenous IL-12 to drive NK cell IFN-gamma production and downstream antiviral defenses during innate immune responses ... The reduction in ability of splenic populations to produce IL-12 induced IFN-gamma after infection by LCMV was associated with decreased efficacy of administered IL-12 for promoting IFN-gamma dependent antiviral effects in the spleen
Becher et al., J Neuroimmunol 2000 : IL-12 production could also be induced by direct engagement of CD40 on microglia using a CD40 agonist ... IL-12 secretion by microglia is significantly reduced by TNF and IFN-gamma antagonists showing that the IL-12 production is subject to regulation by auto- and paracrine stimuli
Verheyen et al., Scand J Immunol 2000 : IL-10 was suppressive on all the investigated cytokines, while IL-4 interfered with IL-2 induced IFN-gamma and IL-12 production, but was additive to IL-2 in its effect on IL-5 and IL-13
Ito et al., J Immunol 2000 (Leukemia, Radiation-Induced...) : Activation of APC via CD40-CD40 ligand pathway induces up-regulation of costimulatory molecules such as B7 and production of IL-12
Cremer et al., J Immunol 2000 : The constitutive IFN-beta production also resulted in an increased production of IL-12 and IFN-gamma Th1-type cytokines and of the beta-chemokines macrophage-inflammatory protein-1alpha, macrophage-inflammatory protein-1beta, and RANTES
Farrar et al., J Biol Chem 2000 : Although Stat4 is activated by interleukin (IL)-12 in both human and murine T cells, Stat4 is activated by interferon ( IFN ) -alpha only in human, but not murine, CD4 ( + ) T cells
Sun et al., Hum Gene Ther 2000 (Liver Neoplasms, Experimental) : This may be related to the fact that B7-1 changes the mechanisms of antitumor immunity and inhibits IFN-gamma production induced by IL-12 in vivo
Otani et al., Cell Immunol 1999 : To understand the mechanisms underlying the strong induction of IFN-gamma by IL-12/IL-18 in mice, we focused our studies on the IFN-gamma producing cells in various lymphoid organs and tissues and utilized the intracellular cytokine staining method to detect such cells in situ
Ma et al., J Immunol 2000 : IL-10 is not responsible for the TNF-alpha mediated inhibition of IL-12 ... IL-10 is not responsible for the TNF-alpha mediated inhibition of IL-12 ... The major transcriptional factors identified to be involved in the regulation of IL-12 p40 gene expression by LPS and IFN-gamma , i.e., c-Rel, NF-kappaB p50 and p65, IFN regulatory factor-1, and ets-2, were not affected by TNF-alpha when examined by nuclear translocation and DNA binding ... The major transcriptional factors identified to be involved in the regulation of IL-12 p40 gene expression by LPS and IFN-gamma, i.e., c-Rel, NF-kappaB p50 and p65, IFN regulatory factor-1, and ets-2, were not affected by TNF-alpha when examined by nuclear translocation and DNA binding
Franchimont et al., J Immunol 2000 : Inhibition of Th1 immune response by glucocorticoids : dexamethasone selectively inhibits IL-12 induced Stat4 phosphorylation in T lymphocytes ... We first investigated the effect of dexamethasone on IL-12-inducible genes and showed that dexamethasone inhibited IL-12 induced IFN-gamma secretion and IFN regulatory factor-1 expression in both NK and T cells ... This occurred even though the level of expression of IL-12 receptors and IL-12 induced Janus kinase phosphorylation remained unaltered ... However, dexamethasone markedly inhibited IL-12 induced phosphorylation of Stat4 without altering its expression ... We conclude that blocking IL-12 induced Stat4 phosphorylation, without altering IL-4 induced Stat6 phosphorylation, appears to be a new suppressive action of glucocorticoids on the Th1 cellular immune response and may help explain the glucocorticoid induced shift toward the Th2 humoral immune response
Wang et al., J Immunol 2000 : That is, binding of MCP on human monocytes by measles virus hemagglutinin or cross linking by an anti-MCP Ab resulted in IL-12 down-regulation, while binding to MCP by Neisseria on epithelial cells produced a calcium flux
Borges et al., J Pediatr 2000 : Production of IL-12 and IFN-gamma by mononuclear cells from 10 patients with hyper-IgE syndrome in response to a number of stimuli was determined, as well as the effect of IL-12 on IFN-gamma release and cell proliferation
Xing et al., Histol Histopathol 2000 (Mycobacterium Infections) : It has become evident that IL-12 is required not only for Th1 differentiation but also for IFN gamma responses by both T cells and macrophages during intracellular infection
McGuirk et al., Eur J Immunol 2000 (Inflammation...) : Direct anti-inflammatory effect of a bacterial virulence factor : IL-10 dependent suppression of IL-12 production by filamentous hemagglutinin from Bordetella pertussis ... Antibody blocking studies revealed that the inhibitory effect of FHA on IL-12 production was dependent on IL-10
Campbell et al., Eur J Immunol 2000 : IFN-gamma priming augmented Gal-lectin induced IL-12 mRNA expression independent of TNF-alpha and IL-1beta, and was required for IL-12 p70 protein production from macrophages and human peripheral blood mononuclear cells ... IFN-gamma priming augmented Gal-lectin induced IL-12 mRNA expression independent of TNF-alpha and IL-1beta, and was required for IL-12 p70 protein production from macrophages and human peripheral blood mononuclear cells ... IFN-gamma priming augmented Gal-lectin induced IL-12 mRNA expression independent of TNF-alpha and IL-1beta, and was required for IL-12 p70 protein production from macrophages and human peripheral blood mononuclear cells
Du et al., Cell Immunol 2000 : Here we show that superantigen staphylococcal enterotoxin B (SEB) induces IL-12/p40 secretion in macrophages ... SEB induced expression of the IL-12/p40 gene involves activation and nuclear translocation of nuclear factor kappaB (NF-kappaB) ... SEB induced expression of the IL-12/p40 gene involves activation and nuclear translocation of nuclear factor kappaB (NF-kappaB)
Reichmann et al., Infect Immun 2000 (Encephalitis...) : Since the CD40/CD40 ligand (CD40L) interaction is involved in the regulation of macrophage production of interleukin 12 (IL-12) and T-cell production of gamma interferon ( IFN-gamma ), effector cell functions associated with resistance to Toxoplasma gondii, the role of CD40L in immunity to this parasite was assessed
La Flamme et al., J Immunol 2000 : Furthermore, IL-6 also up-regulates IL-10 production which, together with IL-6, negatively regulates IL-12 and IFN-gamma production
Reznikov et al., Proc Natl Acad Sci U S A 2000 : Consistent with these observations, IL-12 , a known inducer of IFN-gamma, augmented IL-1beta induced IFN-gamma but suppressed IL-1beta induced PGE ( 2 ) by 75 %
Kawakami et al., FEMS Immunol Med Microbiol 2000 (Cryptococcosis...) : Administration of neutralizing anti-IFN-gamma monoclonal antibody ( mAb ) clearly inhibited production of IFN-gamma and IL-18 induced by IL-12 , while control IgG did not show such an effect ... Administration of neutralizing anti-IFN-gamma monoclonal antibody ( mAb ) clearly inhibited production of IFN-gamma and IL-18 induced by IL-12 , while control IgG did not show such an effect
Solana et al., Vaccine 2000 (Infection) : Furthermore early IFN-gamma and chemokine production in response to IL-2 or IL-12 is also decreased
Haskó et al., Br J Pharmacol 2000 : Pretreatment of the peritoneal macrophages with ATP or various ATP analogues decreased both IL-12 and TNF-alpha production induced by LPS ( 10 microgram ml(-1) ) ... Immunoneutralization with an anti-IL-10 antibody demonstrated that although ATP increases IL-10 production, the inhibition of IL-12 and TNF-alpha production is independent of the increased IL-10
Joosten et al., Ann Rheum Dis 2000 (Acute Disease...) : Enhancement of IL10 and IL1Ra by anti-IL12 was confirmed in a tissue chamber model with SCW induced inflammation ... Enhancement of IL10 and IL1Ra by anti-IL12 was confirmed in a tissue chamber model with SCW induced inflammation
Heath et al., J Immunol 2000 : Thus, although the IL-12R beta 2 and IL-12 dependent STAT4 activation are required for Th1 responses, activation of this pathway is not sufficient to restore a Th1 phenotype in developing or committed Th2 cells
Kikkawa et al., J Interferon Cytokine Res 2000 : We compared the structural and functional properties of three recombinant human interleukin-18 ( rIL-18 ) preparations, commercially available ( Pep rIL-18 ) and prepared in our laboratory ( active and inactive, according to their ability to potentiate IL-12 mediated interferon-gamma [IFN-gamma ] induction in lymphocytes )
Maestroni et al., Adv Exp Med Biol 1999 (Breast Neoplasms...) : The finding that MLT stimulates IL-12 production from human monocytes only if incubated in presence of IL-2 further supports this concept ... The finding that MLT stimulates IL-12 production from human monocytes only if incubated in presence of IL-2 further supports this concept
Aleman et al., Scand J Immunol 2000 (Leprosy) : So, the inhibitory effect of IL-4 on either production of IFN-gamma, TNF-alpha and/or IL-12 or their receptors could be the mechanism underlying the lack of the hsp65 CTL generation in cells from MB
Rieser et al., Urol Int 1999 (Carcinoma, Renal Cell...) : Exogenous IL-12 enhanced and exogenous IL-4 diminished IFN-gamma production
Boyaka et al., Immunol Res 1999 : Serum antigen-specific antibody ( Ab ) responses were enhanced when either IL-6 or IL-12 was mucosally administered with a protein antigen, while only IL-12 induced antigen-specific mucosal IgA Ab responses
Iellem et al., Eur J Immunol 2000 : IL-12 and IFN-alpha, cytokines that promote the differentiation of human Th1 cells, selectively inhibit secretion and mRNA expression of MDC and I-309 by Th1 cells
Chang et al., Eur J Immunol 2000 (Encephalomyelitis, Autoimmune, Experimental) : The costimulatory effect of IL-18 on the induction of antigen-specific IFN-gamma production by resting T cells is IL-12 dependent and is mediated by up-regulation of the IL-12 receptor beta2 subunit
Thomas et al., Int J Cancer 2000 (Carcinoma, Squamous Cell...) : Our results provide evidence that regression of oral head-and-neck SCC may be induced by therapy with systemic IL-12 and that expression of the CD80 co-stimulatory molecule by SCC and IFN-gamma by the host promote IL-12 induced regression of SCC ... Our results provide evidence that regression of oral head-and-neck SCC may be induced by therapy with systemic IL-12 and that expression of the CD80 co-stimulatory molecule by SCC and IFN-gamma by the host promote IL-12 induced regression of SCC
Lee et al., Oncol Rep 2000 (Breast Neoplasms...) : IFN-gamma was rapidly induced by E75 within 20-24 h, in five of six healthy donors, in the presence of IL-12 and was detectable as early as 6 h ... IFN-gamma was also detected, from freshly isolated, unstimulated PBMC in response to HLA-A2 matched tumors + IL-12 but not of IL-12 alone
Frucht et al., J Immunol 2000 (Arthritis, Rheumatoid) : Monocyte expressed Stat4 in humans is phosphorylated in response to IFN-alpha, but not IL-12
Rottenberg et al., J Immunol 2000 (Chlamydia Infections...) : In turn, IFN-gamma is necessary for the increased IL-12p40 mRNA accumulation that occurs in lungs during infection with C. pneumoniae, suggesting a positive feedback regulation between these two cytokines
Fischer et al., J Immunol 2000 (Chronic Disease...) : IL-12 production was up-regulated by external IFN-gamma but was stopped by inhibiting parasite replication
Mason et al., Alcohol Clin Exp Res 2000 : This work describes the effect of exposure to ethanol on lipopolysaccharide (LPS) induced production of IL-12 in mice and whether ethanol induced increases in IL-10 mediates these changes in IL-12 production ... Ethanol suppresses the production of murine IL-12 in response to LPS in blood and lung, with both the p70 and the p40 components affected ... IL-10 may play a role in ethanol induced suppression of IL-12 , as neutralization of IL-10 partially attenuated the suppression of IL-12
Tada et al., J Immunol 2000 : Granulocyte/macrophage colony stimulating factor inhibits IL-12 production of mouse Langerhans cells ... Surprisingly, GM-CSF strikingly inhibited IL-12 p40 production by anti-CD40/IFN-gamma stimulated LC ( % inhibition = 97.0 +/- 0.9 % at 1 ng/ml GM-CSF ) ... IL-1alpha ( 1 ng/ml ) -stimulated KC produced much more GM-CSF than unstimulated KC ( 60.9 +/- 0.2 pg/ml vs 20.9 +/- 1.7 pg/ml ), and IL-1alpha stimulated KC supernatants strongly inhibited IL-12 p40 production by anti-CD40/IFN-gamma stimulated LC ( % inhibition = 89.4 +/- 1.4 % )
Pacocha et al., J Interferon Cytokine Res 2000 : Finally, whereas IFN-beta inhibited antigen-driven generation of IL-5, IL-12 , IL-13, and IFN-gamma, IFN-beta enhanced generation of both IL-4 and IL-10 ... Finally, whereas IFN-beta inhibited antigen-driven generation of IL-5, IL-12 , IL-13, and IFN-gamma, IFN-beta enhanced generation of both IL-4 and IL-10
Lee et al., J Interferon Cytokine Res 2000 (Breast Neoplasms) : We found that IFN-gamma alone was a weak inducer of IP-10 in these cells, whereas IL-12 was a significantly stronger inducer of IP-10 ... In the presence of IL-12 , the tumor peptide E75 ( HER-2, 369-377 ) was a stronger inducer of IP-10 than was IL-12 alone
d'Ostiani et al., J Exp Med 2000 : In vitro, ingestion of yeasts activated dendritic cells for interleukin (IL)-12 production and priming of T helper type 1 ( Th1 ) cells, whereas ingestion of hyphae inhibited IL-12 and Th1 priming, and induced IL-4 production
Gollob et al., Clin Cancer Res 2000 (Arthralgia...) : The down-modulation of IFN-gamma induction during rhIL-12 treatment did not relate to IL-10 production or alterations in rhIL-12 bioavailability but was associated with an acquired defect in lymphocyte IFN-gamma production in response to IL-12 , IL-2, or IL-15
Hessle et al., Infect Immun 2000 : Gram positive bacteria induced much more IL-12 than did gram negative bacteria ( median, 3,500 versus 120 pg/ml at an optimal dose of 25 bacteria/cell ; P < 0.001 ), whereas gram negative bacteria preferentially stimulated secretion of IL-10 ( 650 versus 200 pg/ml ; P < 0.001 )
Marovich et al., J Immunol 2000 (Leishmaniasis, Cutaneous) : However, using intracellular staining and flow cytometry to analyze cytokine production at the single-cell level, we found that Lm-harboring DC, but not monocytes, produce large amounts of IL-12p70 in a CD40 ligand (CD40L) dependent manner
Wu et al., Eur J Immunol 2000 : Using an antibody specific for the human IL-12Rbeta2 subunit, the effect of IFN-gamma , IFN-alpha, IL-12 and IL-2 on the regulation of IL-12R expression and IL-12 responsiveness of human T and NK cells was assessed ... Using an antibody specific for the human IL-12Rbeta2 subunit, the effect of IFN-gamma, IFN-alpha, IL-12 and IL-2 on the regulation of IL-12R expression and IL-12 responsiveness of human T and NK cells was assessed ... Using an antibody specific for the human IL-12Rbeta2 subunit, the effect of IFN-gamma, IFN-alpha, IL-12 and IL-2 on the regulation of IL-12R expression and IL-12 responsiveness of human T and NK cells was assessed
Athié-M et al., Eur J Immunol 2000 (MAP Kinase Signaling System) : IL-12 selectively regulates STAT4 via phosphatidylinositol 3-kinase and Ras independent signal transduction pathways ... IL-12 induces sustained activation and nuclear translocation of STAT4 and this regulatory process is coupled to both tyrosine and serine phosphorylation of this molecule ... Moreover, IL-12 activation of STAT4 in human peripheral blood derived T cells is not accompanied by stimulation of the Ras guanine nucleotide binding cycle or stimulation of MAPK Erk1,2 or initiation of the PI3K signaling pathways ... This reveals that the STAT1, 3 and 4 serine kinases are not coordinately regulated in human T cells and that IL-12 must regulate serine phosphorylation of STAT4 by a kinase network distinct to the MAPK Erk1,2 or PI3K pathways
Nguyen et al., Eur J Immunol 2000 : Inhibition of IL-12 induced up-regulation of CD25 by a p38 mitogen activated protein ( MAP ) kinase inhibitor, SB203580, also ablates the synergism between IL-12 and Q126D
Fukao et al., Eur J Immunol 2000 : IL-2 augmented IL-12 dependent IFN-gamma production by DC purified from both splenocytes of wild-type and anti-asialoGM1 Ab-treated Rag-2 ( -/- ) splenocytes devoid of T, B, NK and NKT cells
Chougnet et al., J Infect Dis 2000 (HIV Infections...) : CBMC IL-12 levels were increased by interferon-gamma and CD40 ligand but remained deficient, compared with PBMC ... CBMC IL-12 levels were increased by interferon-gamma and CD40 ligand but remained deficient, compared with PBMC
Pyeon et al., J Virol 2000 : Type 1 cytokines ( interleukin-2 [IL-2 ], IL-12 , and gamma interferon ) increase in freshly isolated peripheral blood mononuclear cells ( PBMCs ) of animals with an early disease stage of bovine leukemia virus ( BLV ) infection, while IL-10 increases in animals with a late disease stage
de Haan et al., Transplantation 2000 : In this assay also the effects of anti-CD28 antibody and interleukin- (IL) 1beta , IL-6, or IL-12 mediated costimulation on responder T cell proliferation and IL-2 production were investigated
Kohka et al., J Immunol 2000 : The addition of recombinant human IL-18 to the culture concentration-dependently stimulated IL-12 and IFN-gamma production and inhibited the IL-2 and IL-10 production ... The addition of recombinant human IL-18 to the culture concentration-dependently stimulated IL-12 and IFN-gamma production and inhibited the IL-2 and IL-10 production
Flieger et al., Cytokine 2000 (Colonic Neoplasms) : In contrast, IL-4 significantly suppressed the IL-2, IL-12 and IFN-alpha induced ADCC ... In addition, the immunosuppressive cytokine IL-10 in higher concentrations significantly suppressed the IL-12-induced-ADCC
Seo et al., Clin Exp Immunol 2000 (Epstein-Barr Virus Infections...) : Freshly isolated leukaemic NK cells transcribed mRNA for CD94 associated molecule NKG2C at an abnormally high level, and upon stimulation with IL-2 and/or IL-12 they expressed NKG2A as well
Nagayama et al., J Immunol 2000 : We analyzed the expression of IL-12Rbeta1 and IL-12Rbeta2 and the role of IL-12 in the activation of monocyte derived dendritic cells (DCs) via IL-12Rbeta1 mediated signaling events ... Furthermore, stimulation of DCs with IL-12 induced IL-12p40 transcript, but not IL-12p35 transcript, whereas this stimulation caused the expressions of both transcripts in Con A blasts ... Furthermore, stimulation of DCs with IL-12 induced IL-12p40 transcript, but not IL-12p35 transcript, whereas this stimulation caused the expressions of both transcripts in Con A blasts ... Furthermore, stimulation of DCs with IL-12 induced IL-12p40 transcript, but not IL-12p35 transcript, whereas this stimulation caused the expressions of both transcripts in Con A blasts ... Stimulation of DCs with IL-12 caused a tyrosine phosphorylation of several intracellular proteins, and the pattern of these events were distinct from those of IL-12 stimulated Con A blasts ... IL-12 also induced tyrosine phosphorylation of IL-12Rbeta1 as well as recruitment of several tyrosine phosphorylated proteins to IL-12Rbeta1 in DCs and Con A blasts ... IL-12 also induced tyrosine phosphorylation of IL-12Rbeta1 as well as recruitment of several tyrosine phosphorylated proteins to IL-12Rbeta1 in DCs and Con A blasts ... These results suggest that IL-12 acts directly on DCs to induce their functional activation via IL-12Rbeta1 mediated signaling events
Wang et al., J Immunol 2000 : An IFN-gamma-inducible transcription factor , IFN consensus sequence binding protein ( ICSBP ), stimulates IL-12 p40 expression in macrophages
Wang et al., J Immunol 2000 (Multiple Sclerosis) : IFN-beta-1b inhibits IL-12 production in peripheral blood mononuclear cells in an IL-10 dependent mechanism : relevance to IFN-beta-1b therapeutic effects in multiple sclerosis ... IL-12 is a proinflammatory cytokine secreted by dendritic cells in response to microbial Ags and mitogens
Gherardi et al., J Virol 2000 : Moreover, at certain viral doses, coadministration of the NO inhibitor during the booster resulted in IL-12 mediated enhancement of the specific CD8 ( + ) T-cell response
Matsumoto et al., Nephron 2000 (Nephrosis, Lipoid) : Taken together, our results indicate that both IL-18 and IL-12 contribute to the VPF production in vitro and suggest that they play key roles in the complexity of cytokine regulation in the pathophysiology of VPF
De et al., Clin Immunol 2000 (Burns...) : Exogenous IL-12 addition did not restore IFNgamma responses, but exogenous IL-2 partially restored IL-4, IFN-gamma, and IL-10 production ... Exogenous IL-12 addition did not restore IFNgamma responses, but exogenous IL-2 partially restored IL-4, IFN-gamma, and IL-10 production ... Exogenous IL-12 addition did not restore IFNgamma responses, but exogenous IL-2 partially restored IL-4 , IFN-gamma, and IL-10 production
Salgado et al., Cytokine 2000 : The fact that translation and probably translocation of CD26 toward the cell surface can be regulated by IL-12 and TNF-alpha reveals new aspects about the control of this T ( H1 ) marker
Vidalain et al., EMBO J 2000 : We demonstrate that stimulation of Src family kinase within membrane rafts initiates a pathway implicating ERK activation, which leads to interleukin (IL)-1alpha/beta and IL-1Ra mRNA production and contributes to p38 dependent IL-12 mRNA production
Milano et al., Scand J Immunol 2000 (Boutonneuse Fever) : IL-12 secretion by in vitro Ag-stimulated PBMC from BF patients was potentiated by recombinant interferon gamma (IFN-gamma) or anti-IL-10 monoclonal antibodies ( MoAbs )
McRae et al., Blood 2000 : In addition, IFN-alpha/beta treated DC secreted less IL-12 upon stimulation with Staphylococcus aureus Cowan strain or with CD4 ( + ) T cells, and this decrease correlated directly with their inability to support CD4 ( + ) T-cell secretion of IFN-gamma, even though T-cell lymphotoxin production was unaffected
Sawada-Hase et al., Am J Gastroenterol 2000 (Colitis, Ulcerative...) : The present study examined the expression of CD40 in peripheral blood mononuclear cells ( PBMNCs ) and tissue specimens, and CD40 stimulated interleukin (IL)-12 release from PBMNCs in IBD
Roth et al., Thorax 2000 : The compound inhibited serum induced IL-12 expression in peripheral blood lymphocytes but did not induce OSM, LIF, or CNTF at any concentration ... The compound inhibited serum induced IL-12 expression in peripheral blood lymphocytes but did not induce OSM, LIF , or CNTF at any concentration ... The compound inhibited serum induced IL-12 expression in peripheral blood lymphocytes but did not induce OSM , LIF, or CNTF at any concentration
Zhang et al., J Immunol 2000 : The p38 mitogen activated protein kinase is required for IL-12 induced IFN-gamma expression ... In this report we demonstrate that IL-12 activates mitogen activated protein kinase kinase 3/6 ( MKK ) and p38 mitogen activated protein kinase ( MAPK ), but not p44/42 ( ERK ) or stress activated protein kinase/c-Jun N-terminal kinase MAPK ... In this report we demonstrate that IL-12 activates mitogen activated protein kinase kinase 3/6 ( MKK ) and p38 mitogen activated protein kinase ( MAPK ), but not p44/42 ( ERK ) or stress activated protein kinase/c-Jun N-terminal kinase MAPK
Subauste et al., J Immunol 2000 : Human dendritic cells discriminate between viable and killed Toxoplasma gondii tachyzoites : dendritic cell activation after infection with viable parasites results in CD28 and CD40 ligand signaling that controls IL-12 dependent and -independent T cell production of IFN-gamma ... In addition, this IFN-gamma response was dependent in part on IL-12 secretion ... Taken together, T. gondii induced activation of human DC is associated with T cell production of IFN-gamma through CD40-CD40L dependent release of IL-12 and through CD80/CD86-CD28 and CD40-CD40L signaling that mediate IFN-gamma secretion even in the absence of bioactive IL-12 ... Taken together, T. gondii induced activation of human DC is associated with T cell production of IFN-gamma through CD40-CD40L dependent release of IL-12 and through CD80/CD86-CD28 and CD40-CD40L signaling that mediate IFN-gamma secretion even in the absence of bioactive IL-12
Yamauchi et al., J Immunol 2000 (Leprosy, Lepromatous...) : Cognate recognition of M. leprae Ag by a T cell clone derived from a tuberculoid lesion in the context of monocyte APC resulted in CD40L-CD40 dependent production of IL-12 ... Cognate recognition of M. leprae Ag by a T cell clone derived from a tuberculoid lesion in the context of monocyte APC resulted in CD40L-CD40 dependent production of IL-12 ... In contrast, M. leprae induced IL-12 production by PBMC from lepromatous patients was not dependent on CD40L-CD40 ligation, nor was CD40L up-regulated by M. leprae ... In contrast, M. leprae induced IL-12 production by PBMC from lepromatous patients was not dependent on CD40L-CD40 ligation, nor was CD40L up-regulated by M. leprae ... These data suggest that T cell activation in situ by M. leprae in tuberculoid leprosy leads to local up-regulation of CD40L, which stimulates CD40 dependent induction of IL-12 in monocytes
Dybul et al., J Immunol 2000 (HIV Infections) : CD40 ligand trimer and IL-12 enhance peripheral blood mononuclear cells and CD4+ T cell proliferation and production of IFN-gamma in response to p24 antigen in HIV infected individuals : potential contribution of anergy to HIV-specific unresponsiveness
Hong et al., Eur Cytokine Netw 2000 (Diabetes Mellitus, Type 1) : Although IL-12 induces IL-18R expression in Th1 and B lymphocytes, 24-hours rmIL-12 preincubation neither sensitized islets to effects of 10 nM of rm or rrIL-18 alone nor primed the islets to IL-1 beta actions on insulin release and NO production
Balashov et al., Neurology 2000 (Multiple Sclerosis, Chronic Progressive...) : Defective regulation of IFNgamma and IL-12 by endogenous IL-10 in progressive MS ... Thus, the defect in regulation of both IL-12 and IFNgamma production by endogenous IL-10 in progressive MS could be an important factor involved in the transition of MS from the relapsing to the progressive stage and has implications for treating MS patients with exogenous IL-10
Alleva et al., Diabetes 2000 (Diabetes Mellitus, Type 1) : Moreover, NOD Mo IL-12 expression appeared to be near maximally induced by lipopolysaccharide (LPS) alone, because it was only slightly enhanced by the addition of gamma-interferon, a stimulus that substantially elevated LPS induced IL-12 production in Mo from normal strains
Roy et al., Surgery 2000 : Interleukin-4 (IL-4) treatment after lipopolysaccharide (LPS) induction inhibits macrophage ( Mphi ) IL-12 synthesis ; however, IL-4 pretreatment ( PreTx ) primes the Mphi for increased LPS induced IL-12 production ... Overexpression of c-fos completely inhibited IL-4 primed LPS induced IL-12 p70 protein synthesis
Kaliński et al., J Immunol 2000 : However, in contrast to IFN-gamma, IL-4 inhibits the CD40 ligand induced production of inactive IL-12p40 and the production of either form of IL-12 induced by LPS, which may explain the view of IL-4 as an IL-12 inhibitor ... However, in contrast to IFN-gamma, IL-4 inhibits the CD40 ligand induced production of inactive IL-12p40 and the production of either form of IL-12 induced by LPS, which may explain the view of IL-4 as an IL-12 inhibitor ... However, in contrast to IFN-gamma, IL-4 inhibits the CD40 ligand induced production of inactive IL-12p40 and the production of either form of IL-12 induced by LPS , which may explain the view of IL-4 as an IL-12 inhibitor
Sareneva et al., J Immunol 2000 : IFN-alpha and IL-12 induce IL-18 receptor gene expression in human NK and T cells
Isogai et al., Eur Respir J 2000 (Respiratory Hypersensitivity) : Interferon-gamma increases IL-12 mRNA expression and attentuates allergic late-onset airway responses in the Brown Norway rat
Takeshita et al., Eur J Immunol 2000 : C/EBPbeta also contributed to CpG ODN mediated IL-12 p40 promoter activation
Ruedl et al., Eur J Immunol 2000 : CD40-CD40L interaction was essential for IL-12 production by DC ... CD40-CD40L interaction was essential for IL-12 production by DC
DeCicco et al., J Infect Dis 2000 (Vitamin A Deficiency) : Conversely, IL-12 and IL-10 mRNAs were elevated in VA deficiency and were induced by PIC and suppressed by RA
Varga et al., J Immunol 2000 (Asthma) : IL-5 production by BAL T cells and PBMC was inhibited by IL-12 and, to a lesser extent, by IFN-gamma ... IL-12 also induced IFN-gamma production by BAL T cells and PBMC ... The effects of IL-12 nor IFN-gamma on IL-5 production could not be reversed by neutralizing anti-IFN-gamma or anti-IL-12 mAbs, respectively
Nakayama et al., J Invest Dermatol 2000 : P-selectin glycoprotein ligand-1 and Core 2 beta1, 6-N-acetylglucosaminyltransferase were progressively up-regulated by repeated IL-12 stimulation, but they were not downregulated by IL-4 ... This suggested that alpha1,3-fucosyltransferase VII is transcriptionally regulated directly by IL-12 and IL-4
Verhagen et al., J Exp Med 2000 (Mycobacterium avium-intracellulare Infection...) : IL-12 induced IFN-gamma production in IL-12Rbeta1-deficient T cells could be inhibited by the p38 mitogen activated protein kinase ( MAP ) kinase inhibitor SB203580 and the MAP kinase kinase (MEK) 1/2 inhibitor U0126, suggesting involvement of MAP kinases in this alternative, Stat4 independent, IL-12 signaling pathway.Collectively, these results indicate that IL-12 acts as a partial agonist in the absence of IL-12Rbeta1
Shigehara et al., Sarcoidosis Vasc Diffuse Lung Dis 2000 (Sarcoidosis, Pulmonary) : These results may indicate that IL-12 induces IFN-gamma expression and subsequent Th1 dominant condition in Japanese patients with sarcoidosis
Pisetsky et al., Clin Immunol 2000 : The Ps ODNs also blocked IL-12 production induced by lipopolysaccharide (LPS) and a stimulatory Ps ODN ... With the J774 cell line, single-base Ps ODNs inhibited IL-12 production induced by bacterial DNA, LPS , and a stimulatory Ps ODN
Matsui et al., Clin Exp Allergy 2000 (Hypersensitivity, Immediate) : Reduced IFNgamma production in response to IL-12 stimulation and/or reduced IL-12 production in atopic patients ... Several studies have shown that interleukin (IL)-4 and interferon-gamma (IFNgamma) are important for the regulation of IgE production and that IL-12 induces IFNgamma ... Several studies have shown that interleukin (IL)-4 and interferon-gamma (IFNgamma) are important for the regulation of IgE production and that IL-12 induces IFNgamma ... IFNgamma production in response to IL-12 stimulation and IL-12 production were investigated in peripheral blood mononuclear cells ( PBMCs ) of atopic patients with various levels of serum IgE ... It was shown that the elevation in IgE levels in atopic patients may be due to reduced IFNgamma production in response to IL-12 stimulation and/or due to reduced IL-12 production
Wilkinson et al., Scand J Immunol 2000 (Carcinoma...) : A regulatory role for interleukin (IL)-12 in production of transforming growth factor (TGF)-beta1 has been suggested, however, remains controversial ... In this study, we analyzed the effect of IL-12 on TGF-beta1 expression in the human lines, K562 and A549, and in primary human monocytes and macrophages
Chen et al., J Immunol 2000 : By contrast, intra-Peyer 's patch IL-12 gene transfer completely restored T cell proliferation and IFN-gamma secretion and partially reversed IL-2 inhibition ... B7 selectively increased T cell proliferation and IL-2 secretion without affecting IFN-gamma production, whereas IL-12 increased both IL-2 and IFN-gamma production ... B7 selectively increased T cell proliferation and IL-2 secretion without affecting IFN-gamma production, whereas IL-12 increased both IL-2 and IFN-gamma production
Schmitt et al., J Immunol 2000 : We suggest that by suppressing DC/Mphi IL-12p70 secretion while promoting IL-12p40 homodimer secretion, UV exposure preferentially suppress the activation of Th1 cells, thereby suppressing Th-1 cell-driven inflammatory immune reactions ... We suggest that by suppressing DC/Mphi IL-12p70 secretion while promoting IL-12p40 homodimer secretion, UV exposure preferentially suppress the activation of Th1 cells, thereby suppressing Th-1 cell-driven inflammatory immune reactions
Elhofy et al., J Immunol 2000 (Salmonella Infections, Animal) : Expression of high affinity IL-12 receptors is required for IL-12 mediated IFN-gamma production ... Attenuated IL-12Rbeta2 mRNA expression correlated with reduced receptor signaling, as demonstrated by reduced IL-12 induced STAT4 phosphorylation in enriched T lymphocytes isolated from the mesenteric lymph nodes and spleens of Salmonella infected mice
Yang et al., Cytokines Cell Mol Ther 2000 (Graft vs Host Disease) : Interestingly, these inhibitory effects are also mediated by IFN-gamma , whose production is induced by IL-12
Sobel et al., Diabetologia 2000 (Diabetes Mellitus, Type 1) : Cyclophosphamide treatment decreased IL-12 , IL-1beta, IL-2, IFN-gamma and TNF-alpha gene expressions in mononuclear spleen cells but IL-4 gene expression increased
Marcinkiewicz et al., Immunopharmacology 2000 : PTX also affects the production of other cytokines such as IL-1, IL-6, IL-10, IL-12 , and IFN-gamma
Okamoto et al., Immunopharmacology 2000 : In in vitro experiments using human peripheral blood mononuclear cells ( PBMC ), OK-PSA induced IFN-gamma, interleukin (IL)-2, IL-12 , IL-18, tumor necrosis factor (TNF)-alpha and TNF-beta that are generally called `` Th1-type cytokines '' both in protein and in mRNA levels
Nicoletti et al., Hepatology 2000 (Hepatitis, Autoimmune) : Thus, IL-12 induced production of IFN-gamma might play a role in mediating the hepatitis inducing effect of ConA
Willermain et al., Invest Ophthalmol Vis Sci 2000 : Although interferongamma enhanced IL-6 and IL-8 production, CD40 triggering of IFNgamma activated hRPE cells did not induce IL-12 secretion ... Although interferongamma enhanced IL-6 and IL-8 production, CD40 triggering of IFNgamma activated hRPE cells did not induce IL-12 secretion ... Although interferongamma enhanced IL-6 and IL-8 production, CD40 triggering of IFNgamma activated hRPE cells did not induce IL-12 secretion ... Although interferongamma enhanced IL-6 and IL-8 production, CD40 triggering of IFNgamma activated hRPE cells did not induce IL-12 secretion
Schönlau et al., Eur J Immunol 2000 (Leishmaniasis) : CD18 is also involved in reduction of IL-12 release by L. major infected macrophages, as uptake of opsonized parasites ( via CR3 ) decreased IL-12 release only in WT, but not in CD18-/- macrophages
Ozaki et al., Clin Exp Immunol 2000 (Blepharitis...) : Interferon-gamma production was augmented by IL-12
Liu et al., Immunology 2000 : The results showed that CGRP attenuated LPS induced IL-12 release in a concentration dependent manner ... The results showed that CGRP attenuated LPS induced IL-12 release in a concentration dependent manner ... However, CGRP had no effect on IL-12 production from unstimulated Mphi ... The LPS induced IL-12 release from Mphi could also be reduced by forskolin, an activator of adenylate cyclase , and 8-Br-cAMP, an analogue of cAMP ... The LPS induced IL-12 release from Mphi could also be reduced by forskolin, an activator of adenylate cyclase, and 8-Br-cAMP, an analogue of cAMP ... Using the reverse transcription-polymerase chain reaction ( RT-PCR ), we found that CGRP also decreased the LPS induced IL-12 p40 mRNA levels ... These data suggest that LPS induced IL-12 release and gene expression were attenuated by CGRP via an activated cAMP-protein kinase A (PKA) pathway in mouse peritoneal Mphi ... These data suggest that LPS induced IL-12 release and gene expression were attenuated by CGRP via an activated cAMP-protein kinase A (PKA) pathway in mouse peritoneal Mphi
Biasin et al., J Infect Dis 2000 : However, interleukin (IL)-6, IL-10, IL-12 , interferon (IFN)-gamma, and tumor necrosis factor (TNF)-alpha and -beta mRNA were elevated in PBMC and cervical mucosa biopsy specimens of ESN and HIV infected women ; CCR5 and CXCR4 mRNA were augmented in cervical mucosa biopsy specimens, but not in PBMC, of ESN and HIV infected women ; HIV-specific IFN-gamma secreting cells were detected in vaginal washes of ESN and HIV infected women ; and phenotypic alterations were present in PBMC of ESN women
Murray et al., J Infect Dis 2000 (Leishmaniasis, Visceral) : IL-12 plus Sb synergism in normal mice was IFN-gamma dependent ; however, IL-12 also increased responsiveness to Sb in IFN-gamma KO mice
Swinburne et al., Cytokine 2000 : Interleukin 12 (IL-12) is a heterodimeric cytokine composed of two subunits that form the biologically active p70 molecule, and is a potent inducer of the pro-inflammatory cytokine IFN-gamma
Haskó et al., FASEB J 2000 : Adenosine inhibits IL-12 and TNF-[alpha ] production via adenosine A2a receptor dependent and independent mechanisms ... Although adenosine increases IL-10 production, the inhibition of IL-12 production is independent of the increased IL-10
Sur et al., Exp Lung Res 2000 (Asthma...) : Whereas both mucosal and systemic IL-12 inhibited BAL eosinophil recruitment at high doses ( 100-1000 ng ), only mucosal IL-12 was effective at low doses ( 1-10 ng )
Thoma-Uszynski et al., J Immunol 2000 : Activation of toll-like receptor 2 on human dendritic cells triggers induction of IL-12 , but not IL-10 ... Upon stimulation with bLP or LPS, monocytes produced IL-12 and IL-10 at similar levels, whereas monocyte derived DC produced comparable levels of IL-12, but little IL-10 ... Upon stimulation with bLP or LPS , monocytes produced IL-12 and IL-10 at similar levels, whereas monocyte derived DC produced comparable levels of IL-12, but little IL-10 ... Greater than 90 % of the bLP induced production of IL-12 was blocked by anti-TLR2 mAb
Gollob et al., J Immunol 2000 (Immunologic Deficiency Syndromes...) : An examination of cytokine signaling revealed that STAT4 and extracellular regulated kinase 1 (ERK1) activation by IL-12 was intact, whereas the activation of STAT1 , -3, and -5 by IL-12 was lost ... An examination of cytokine signaling revealed that STAT4 and extracellular regulated kinase 1 (ERK1) activation by IL-12 was intact, whereas the activation of STAT1, -3, and -5 by IL-12 was lost ... An examination of cytokine signaling revealed that STAT4 and extracellular regulated kinase 1 (ERK1) activation by IL-12 was intact, whereas the activation of STAT1, -3, and -5 by IL-12 was lost ... These findings demonstrate that the activation of STAT4 alone is not sufficient for IL-12 induced IFN-gamma production and proliferation and suggest that other STATs play a role in these responses to IL-12
Whittaker et al., J Immunol 2000 : Blocking COX-2 reduces I-MDC secretion of IL-12p40, whereas it increases IL-12p40 and p70 production by maturing MDC
Leite-de-Moraes et al., J Immunol 2000 : This process was preceded by up-regulation of Fas ( CD95 ) and Fas ligand expression in response to IL-12 plus IL-18 and was blocked by zVAD, a large spectrum caspase inhibitor, as well as by anti-Fas ligand mAb, suggesting the involvement of the Fas pathway
Zou et al., J Immunol 2000 : Neutralization of MDDC, but not PhiDC IL-12 significantly inhibited T cell IFN-gamma induction
O'Regan et al., J Leukoc Biol 2000 : Herein, we demonstrate that osteopontin stimulation augments the ability of anti-CD3 monoclonal antibody to induce CD40 ligand (CD40L) and IFN-gamma expression on human T cells, resulting in CD40L- and IFN-gamma dependent IL-12 production in vitro ... These findings suggest a functional role for osteopontin in early Th1 responses, namely regulation of T cell dependent IL-12 production
Pien et al., J Immunol 2000 (Genetic Predisposition to Disease...) : During murine cytomegalovirus infections known to target spleen and liver, IL-12 induced IFN-gamma from NK cells is crucial for resistance
Dickensheets et al., J Interferon Cytokine Res 2000 : Although purified resting T cells were largely nonresponsive to IL-12 stimulation, anti-CD3 activated T cell blasts were strongly responsive, as demonstrated by the ability of IL-12 to induce Stat4 DNA binding activity ... Thus, IL-12 amplified expression of IFN-gamma and IL-10 and, via its ability to upregulate production of IL-10, inhibited expression of IL-2
Ryan et al., J Immunol 2000 (Disease Models, Animal...) : In contrast, LTK63 augmented the production of IL-12 and TNF-alpha
Lienenlüke et al., Eur J Immunol 2000 : Of note was that the magnitude of CD154 induced IL-12 synthesis in the cultured endothelial cells was comparable to that evoked in freshly isolated human monocytes ... This CD40 mediated induction of endothelial IL-12 synthesis may thus lead to an enhanced activation of the adherent CD154 expressing Th1 cells, thereby fuelling the proinflammatory response
Schulz et al., Immunity 2000 : CD40 ligation triggers IL-12 production by dendritic cells ( DC ) in vitro ... Here, we demonstrate that CD40 cross linking alone is not sufficient to induce IL-12 production by DC in vivo ... Nevertheless, after DC activation by microbial stimuli that primarily upregulate IL-12 p40 and augment CD40 expression, CD40 ligation induces a significant increase in IL-12 p35 and IL-12 p70 heterodimer production
Mosca et al., Blood 2000 : We used combinations of signaling through CD40, using CD40 ligand trimer (CD40L), and interferon gamma to demonstrate that CD83 expression is necessary but not sufficient for optimal production of IL-12 by DCs
Fantuzzi et al., J Leukoc Biol 2000 (Inflammation) : IFN-alpha and IL-18 exert opposite regulatory effects on the IL-12 receptor expression and IL-12 induced IFN-gamma production in mouse macrophages : novel pathways in the regulation of the inflammatory response of macrophages ... IL-12 response was regulated differentially by IFN-alpha and IL-18 ... We suggest that differential regulation of IL-12 response by IFN-alpha and IL-18 can represent previously unrecognized regulatory mechanisms for maintaining suitable levels of differentiation/activation in macrophages
Murthy et al., Infect Immun 2000 : To understand the mechanism ( s ) of IL-10 action during early infection, when innate immunity expressed chiefly by skin macrophages is key, we investigated the effect of exogenous and endogenous IL-10 on the production of the macrophage derived cytokines IL-6, IL-1beta, IL-12 , and tumor necrosis factor alpha (TNF-alpha) ... The inhibition of endogenous IL-10 function by anti-IL-10 antibody reduced the production of IL-12 and IL-6 but not that of IL-1beta and TNF-alpha
Cai et al., Infect Immun 2000 (Toxoplasmosis, Animal) : Since IL-18 is a potent enhancer of IL-12 induced production of IFN-gamma by NK cells, SCID mice ( which lack an adaptive immune response ) were used to assess the role of IL-18 in innate resistance to T. gondii
Wu et al., J Immunol 2000 : To investigate the role of IL-12R beta 2 in IL-12 signaling, we have generated IL-12R beta 2-deficient ( IL-12R beta 2 ( -/- ) ) mice by targeted mutation in embryonic stem ( ES ) cells
Pietrella et al., J Infect Dis 2001 : gp120 inhibited early and late production of interleukin (IL)-12 by PBMC ... Flow cytometric analysis revealed that gp120 down-regulated the expression of IL-12 receptor beta2 subunit on T cells responding to C. neoformans ... Exogenous IL-12 added simultaneously with gp120 up-regulated interferon-gamma secretion and limited IL-4 production ... Exogenous IL-12 added simultaneously with gp120 up-regulated interferon-gamma secretion and limited IL-4 production
Yamaguchi et al., Inflamm Res 2000 : Therefore, we examined the effects of IL-18 and IL-18+IL-12 on HDC activity
Manigold et al., Cytokine 2000 : It mediates LPS/endotoxin lethality by IL-12 independent interferon-gamma synthesis and it induces bacteria related organ failure
Yang et al., Int Immunol 2000 : Requirement for IFN-gamma in IL-12 production induced by collaboration between v ( alpha ) 14 ( + ) NKT cells and antigen presenting cells
Dieli et al., J Exp Med 2000 (Shwartzman Phenomenon) : The generalized Shwartzman reaction in mice which had been primed and challenged with lipopolysaccharide (LPS) depends on interleukin (IL)-12 induced interferon (IFN)-gamma production at the priming stage
Stylianou et al., APMIS 2000 (HIV Infections) : A similar pattern was seen for MAC-PPD stimulated IL-12 levels
Lawless et al., J Immunol 2000 : Stat4 is activated in response to IL-12 ... Thus, Stat4 activation by IL-12 is required for the function of multiple cytokine pathways that result in induction of IFN-gamma
Parrello et al., J Immunol 2000 (Colitis...) : Finally, we showed that stimulation of lamina propria mononuclear cells with IL-12 enhanced IL-12Rbeta2 , suggesting that IL-12 regulates IL-12Rbeta2 expression in human gastrointestinal mucosa
Ediriwickrema et al., Immunology 2000 : DEX induced IFN-gamma production by NK cells was dependent upon the presence of IL-12 , and IL-12 production by M phi was dependent upon the presence of IFN-gamma in these co-cultures ... The results also indicate an IL-12 dependent positive feedback interaction between NK cells and M phi that supports a NK cell/IFN-gamma dependent mechanism for enhancement of anti-BSA IgG2a antibody responses in mice immunized with HMW-DEX-BSA protein conjugates
Kim et al., J Immunol 2001 : In the human NK cell line NKO, IL-18R alpha, and IL-18R beta are expressed constitutively but IL-18 did not induce IFN-gamma unless IL-12 was present ... In the human NK cell line NKO, IL-18R alpha, and IL-18R beta are expressed constitutively but IL-18 did not induce IFN-gamma unless IL-12 was present ... In the human NK cell line NKO, IL-18R alpha, and IL-18R beta are expressed constitutively but IL-18 did not induce IFN-gamma unless IL-12 was present ... In NKO cells and PBMC, IL-12 increased steady-state mRNA levels of IL-18R alpha and IL-18R beta ; the production of IFN-gamma corresponded to IL-12 induced IL-18R alpha and IL-18R beta chains
Perera et al., J Immunol 2001 : In contrast, for optimal induction of COX-2, IL-12 p35, and IL-12 p40 genes by low concentrations of LPS or by all concentrations of Taxol, CD11b/CD18 was also required ... In contrast, for optimal induction of COX-2, IL-12 p35, and IL-12 p40 genes by low concentrations of LPS or by all concentrations of Taxol, CD11b/CD18 was also required
Ebner et al., J Immunol 2001 : 5 ) IL-10 inhibited the production of IL-12
Shigehara et al., J Immunol 2001 (Sarcoidosis, Pulmonary) : To obtain better immunopathologic understanding of sarcoidosis, we examined the expression of IL-12 and IL-18 and their roles in IFN-gamma production in pulmonary sarcoidosis
Aihara et al., Respiration 2000 : We investigated the effects of NAC and AMB on secretions of IL-12 and IL-10 from human alveolar macrophages ... Both NAC and AMB enhanced LPS induced secretion of IL-12 ... Both NAC and AMB enhanced LPS induced secretion of IL-12 ... The ratio IL-12/IL-10 secretion was increased by AMB, but NAC did not affect it
Xing et al., J Leukoc Biol 2000 : In this study, we examined the role of IL-12 in macrophage TNF-alpha and nitric oxide ( NO ) release by using an in vitro model of intracellular infection ... However, IL-12 and mycobacteria together enhanced TNF-alpha and NO release synergistically ... Because IL-12 and mycobacteria also released IFN-gamma from macrophages synergistically, and exogenous IFN-gamma with mycobacteria enhanced TNF-alpha and NO release synergistically, we examined the role of endogenous IFN-gamma in IL-12/mycobacteria stimulated macrophage activation
Kaufmann et al., Immunobiology 2000 (Uterine Cervical Neoplasms) : IL-2 , IL-12 or the combination of IL-2 and IL-7 induced natural killer cell-like activity and specific cytolytic activity against parental and CD80 positive tumor cells
Nakayama et al., Anticancer Res 2000 (Colorectal Neoplasms) : IL-12 may regulate VEGF in the patients diagnosed with colorectal cancer
Van Weyenbergh et al., Immunol Lett 2001 : IFN-beta and TGF-beta differentially regulate IL-12 activity in human peripheral blood mononuclear cells ... We found that IFN-beta by itself induced modest amounts of IFN-gamma, but was able to synergize with IL-12 for IFN-gamma induction ... TGF-beta, on the other hand, had no effect by itself and inhibited significantly the IL-12 induced IFN-gamma secretion ... In conclusion, the ability of IFN-beta to synergize with IL-12 for IFN-gamma synthesis, without significant concomitant IL-10 production, suggest a strong boost to Th1 development, which seems to be IL-18 independent
Leite-De-Moraes et al., J Immunol 2001 : The effect of IL-18 on IL-4 production by activated NKT cells took place both in vivo and in vitro and was not affected by IL-12 which increased IFN-gamma secretion in the same conditions ... The effect of IL-18 on IL-4 production by activated NKT cells took place both in vivo and in vitro and was not affected by IL-12 which increased IFN-gamma secretion in the same conditions
Kane et al., J Immunol 2001 (Disease Progression...) : The IL-10 produced by infected macrophages prevented macrophage activation and diminished their production of IL-12 and TNF-alpha
Walter et al., J Exp Med 2001 (Asthma...) : Initial comparisons indicated that tumor necrosis factor alpha induction of epithelial intercellular adhesion molecule 1 required sequential induction of interleukin (IL)-12 ( p70 ) and interferon gamma, and unexpectedly localized IL-12 production to airway epithelial cells ... Initial comparisons indicated that tumor necrosis factor alpha induction of epithelial intercellular adhesion molecule 1 required sequential induction of interleukin (IL)-12 ( p70 ) and interferon gamma, and unexpectedly localized IL-12 production to airway epithelial cells ... Epithelial IL-12 was also inducible during paramyxoviral bronchitis, but in this case, initial IL-12 p70 expression was followed by 75-fold greater expression of IL-12 p40 ( as monomer and homodimer ) ... Epithelial IL-12 was also inducible during paramyxoviral bronchitis, but in this case, initial IL-12 p70 expression was followed by 75-fold greater expression of IL-12 p40 ( as monomer and homodimer )
la Sala et al., J Immunol 2001 (Inflammation) : ATP inhibition of TNF-alpha and IL-12 production by mature DCs was not mediated by PGs or elevation of intracellular cAMP and did not require ATP degradation
Seki et al., J Immunol 2001 : These results indicate that MyD88 is essential for IL-12 and IL-1beta production from Kupffer cells while their IL-18 secretion is mediated via activation of endogenous caspase-1 without de novo protein synthesis in a MyD88 independent fashion after stimulation with LPS
Wang et al., J Immunol 2001 (Asthma...) : IL-12 dependent vascular cell adhesion molecule-1 expression contributes to airway eosinophilic inflammation in a mouse model of asthma-like reaction
Su et al., Biochem Biophys Res Commun 2001 : IL-12 alone did not activate STAT1 , but IL-12 enhanced IFN-gamma mediated STAT1 phosphorylation in MC-38, MCA102, MCA205, MCA207 and Colon-26-NL-17 cells, which expressed IL-12 receptor beta1 mRNA
Cong et al., Eur J Immunol 2001 : Antibody blocking experiments showed that CT inhibition of IL-12 and TNF-alpha production was mediated by increased IL-10 production, in that addition of anti-IL-10 monoclonal antibody abrogated CT inhibition
Tone et al., Proc Natl Acad Sci U S A 2001 : The interaction between CD40 and CD40 ligand ( CD154 ) activates NF-kappa B, Jun N-terminal kinase, and Janus kinase/signal transducers and activators of transcription pathways and promotes B cell growth, differentiation, and survival as well as IL-12 production in macrophages and dendritic cells ... The interaction between CD40 and CD40 ligand ( CD154 ) activates NF-kappa B, Jun N-terminal kinase, and Janus kinase/signal transducers and activators of transcription pathways and promotes B cell growth, differentiation, and survival as well as IL-12 production in macrophages and dendritic cells
Young et al., AIDS Res Hum Retroviruses 2001 (HIV Seropositivity) : IL-12 increased cell recovery in cultures of PBMC from HIV-seropositive individuals with CD4 counts > 500 cells/microl and also increased the precursor CTL frequency
Affleck et al., J Interferon Cytokine Res 2001 : The role of IL-18 and IL-12 in the induction of IFN-gamma following allogeneic heart transplantation has not been described
Bozza et al., J Interferon Cytokine Res 2001 : In a cell culture model of murine T cell differentiation, rHuIL-11 acts to inhibit IL-2 production as well as IL-12 induced IFN-gamma production and enhances IL-4 and IL-10 production
Matsunaga et al., Infect Immun 2001 : The lipopolysaccharide (LPS) induced production of IL-12 was down-regulated by infection with virulent L. pneumophila, but other cytokines were not affected ... The IL-12 suppression occurred at the level of mRNA accumulation for IL-12 genes in response to LPS stimulation, but the infection induced a marked accumulation of mRNA for both MCP-1 and MCP-3, which are known to suppress IL-12 production in LPS stimulated macrophages ... However, pretreatment of macrophages with MCP-1 did not suppress LPS induced IL-12 production at the concentrations induced by L. pneumophila infection ... These results suggest that L. pneumophila selectively suppresses IL-12 production induced by LPS from macrophages in vitro by an MCP independent mechanism
Elloso et al., Eur J Immunol 2001 : IL-12 is a potent inducer of IFN-gamma production and Th1 responses ... Co-stimulation mediated by B7 has been shown to synergize with IL-12 for optimal IFN-gamma production and proliferation in vitro ... IL-12 induced IFN-gamma production and STAT4 nuclear translocation were markedly reduced in CD28 ( -/- ) splenocytes compared to that of wild-type ( WT ) splenocytes ... IL-12 induced IFN-gamma production and STAT4 nuclear translocation were markedly reduced in CD28 ( -/- ) splenocytes compared to that of wild-type ( WT ) splenocytes ... Analysis of IL-12R expression revealed that IL-12 induced similar levels of IL-12R beta2 mRNA expression in WT and CD28 ( -/- ) cells ... CD28 ( -/- ) CD4 ( + ) but not CD8 ( + ) cells exhibited a defect in IL-12Rbeta1 expression that was associated with a marked decrease in IL-12 binding as well as IL-12 induced IFN-gamma production
Tarte et al., Leukemia 2000 (Multiple Myeloma) : In addition, mature DC obtained with TNF-alpha produced IL-12 and some IL-10 in response to CD40 stimulation
Leceta et al., Ann N Y Acad Sci 2000 (Inflammation) : VIP/PACAP inhibit the production of TNF alpha, IL-6, IL-12 , and nitric oxide ( NO ), and stimulate IL-10 in peritoneal macrophages and Raw 264.7 cells ... VIP/PACAP inhibit the production of TNF alpha, IL-6, IL-12 , and nitric oxide ( NO ), and stimulate IL-10 in peritoneal macrophages and Raw 264.7 cells
Yang et al., Shock 2001 (Myocardial Ischemia...) : We conclude that the production of IL-12 in myocardial reperfusion injury is dramatically affected by the levels of endogenous IL-10
Makrigiannis et al., J Leukoc Biol 2001 : Moreover, IL-12Rbeta2 expression by T cells activated in the presence of anti-CD86 mAb is enhanced by IL-12 ... We, therefore, conclude that the ability of IL-12 to substitute for CD28-costimulatory signaling during CTL development is a result of the interaction of IL-12 with IL-12Rbeta2 induced by low levels of IL-2 synthesized by T cells activated in a CD28 independent manner
Dinarello et al., Eur Cytokine Netw 2000 (Arthritis, Experimental...) : IL-18 and IL-12 act synergistically to stimulate I cells and natural killer cells to produce IFN-gamma but neutralization of IL-18 prevents IL-12 induction of IFN-gamma
Schindler et al., J Immunol 2001 : Here, we demonstrate that STAT4 is indispensable for IL-12/IL-18 induced production of IFN-gamma by mouse peritoneal macrophages ... Type 2 NO synthase ( NOS2 ), which we previously found to be a prerequisite for IL-12 induced IFN-gamma production in NK cells, was not required for IFN-gamma production by these macrophages ... IL-12 alone already induced the expression of IFN-gamma mRNA, but nuclear translocation of STAT4, the release of IFN-gamma protein, and the subsequent production of NO was strictly dependent on the simultaneous presence of IL-18 ... NF-kappa B , which mediates IL-18 effects in T cells, was only weakly activated by IL-12 and/or IL-18 in macrophages
Ropert et al., J Immunol 2001 (Inflammation) : The use of the specific inhibitors of ERK-1/ERK-2 ( PD 98059 ) and p38/SAPK-2 ( SB 203580 ) phosphorylation also indicates the role of MAPKs, with possible involvement of cAMP response element binding protein, in triggering TNF-alpha and IL-12 synthesis by IFN-gamma-primed-macrophages exposed to tGPI or tGPI-mucin
Shiratsuchi et al., Clin Exp Immunol 2001 (Tuberculosis) : Both recombinant human IL-12 ( 77 +/- 42 pg/ml, control versus 1492 +/- 141 pg/ml, cultures with IL-12 1 ng/ml ) and IL-18 ( 126 +/- 37 pg/ml, control versus 2683 +/- 864 pg/ml, cultures with IL-18 10 ng/ml ) augmented M. avium induced IFN-gamma production
Yamamura et al., Arthritis Rheum 2001 (Arthritis, Rheumatoid...) : To examine the levels of interleukin-18 (IL-18) bioactivity within the rheumatoid arthritis ( RA ) joint, and the differential effects of IL-12 and IL-18 on interferon-gamma (IFNgamma) production by T cell infiltrates ... However, IL-12 induced IFNgamma production was synergistically enhanced by IL-18, and yet was > 50 % reduced by neutralization of endogenous IL-18 with anti-IL-18 antibody ... These results indicate that IL-18, produced predominantly by tissue macrophages, primarily potentiates IL-12 induced IFNgamma production by T cell infiltrates in RA synovium
Constantinescu et al., Cytokine 2001 : IL-15, but not IL-12 , induces MMP-9 in PBMC and in T cells
Park et al., J Immunol 2001 : In addition to depending on IFN-gamma, the ability of IL-12 or IL-2/IL-12 to induce Mig and Crg-2 gene expression in purified hepatocytes also is accompanied by the coordinate up-regulation of the IFN-gamma R alpha and beta-chains, in the absence of IL-12R components ... In addition to depending on IFN-gamma, the ability of IL-12 or IL-2/IL-12 to induce Mig and Crg-2 gene expression in purified hepatocytes also is accompanied by the coordinate up-regulation of the IFN-gamma R alpha and beta-chains, in the absence of IL-12R components
Das et al., J Immunol 2001 (Disease Progression...) : mRNA expression and flow cytometric analysis of infected spleen cells suggested that cystatin and IFN-gamma treatment, in addition to greatly reducing parasite numbers, resulted in reduced levels of IL-4 but increased levels of IL-12 and inducible NO synthase
Du et al., J Neuroimmunol 2001 (Encephalomyelitis, Autoimmune, Experimental) : We show that tyrphostin A1 inhibits CD40L stimulated IL-12 production in macrophage cultures and antigen induced generation of Th1 cells
Ozenci et al., J Neuroimmunol 2001 (Multiple Sclerosis) : The functional importance of high IL-12Rbeta2 in MS was underlined by the finding that IL-12 stimulated IFN-gamma production and proliferation of PHA activated T cells correlated with levels of IL-12Rbeta2 expression
Schopf et al., Parasite Immunol 2001 (Disease Models, Animal...) : Interestingly, iNOS was not induced in the lymph node by IL-12 , yet, nitric oxide is critical in the control of leishmaniasis ... However, we observed an increase in iNOS at the lesion site in response to IL-12 ... IL-12 induced iNOS at the lesion site and an early granulomatous inflammation in the lymph node ; therefore, we hypothesize that these are key events responsible for the resolution of disease in BALB/c mice treated with IL-12
Huang et al., Zhonghua Xue Ye Xue Za Zhi 1998 (Leukemia) : IFN-gamma and IL-12 increased the expressions of B7-1, IL-2 and IFN-gamma , but IL-10 inhibited all the expressions ... IFN-gamma and IL-12 increased the expressions of B7-1, IL-2 and IFN-gamma, but IL-10 inhibited all the expressions
Matsunaga et al., Am J Respir Cell Mol Biol 2001 (Legionnaires' Disease) : Analysis of IL-12 p40 responses of both macrophage types to Lp-V infection assessed by reverse transcriptase/polymerase chain reaction revealed induction of increased message levels, but significant levels were induced only slowly
Lin et al., Pediatr Allergy Immunol 2001 : Combining IL-12 and IL-15, however, resulted in decreased CB CD3+ CD8+ expression compared with IL-15 alone
Fukao et al., J Immunol 2001 : Autocrine activation of APC by IL-12 has recently been revealed ; we demonstrate here that inducible expression of Stat4 in APC is central to this process ... Stat4 levels directly correlate with IL-12 dependent IFN-gamma production by APC as well as IFN-gamma production by DC during Ag presentation ... Furthermore, Stat4 ( -/- ) macrophages are defective in NO production in response to IL-12 and are susceptible to TOXOPLASMA : Autocrine IL-12 signaling is required for high-level IFN-gamma production by APC at critical stages in both innate and adaptive immunity, and the control of Stat4 expression is likely an important regulator of this process
Smyth et al., J Exp Med 2001 (Carcinoma, Renal Cell...) : Administration of therapeutic doses of interleukin (IL)-12, a powerful inducer of interferon (IFN)-gamma production by NK cells and NKT cells, upregulated TRAIL expression on liver, spleen, and lung NK cells, and IL-12 suppressed metastases in both liver and lung in a TRAIL dependent fashion
Lantelme et al., Immunology 2001 : During T-cell commitment the presence of IL-4 in the culture was essential to maintain or enhance GATA-3 transcription, while IL-12 was not necessary for full repression of GATA-3
Libraty et al., J Virol 2001 : Activation of the dengue virus infected DCs was blunted compared to the surrounding, uninfected DCs, and dengue virus infection induced low-level release of interleukin-12 p70 (IL-12 p70) , a key cytokine in the development of cell mediated immunity ( CMI )
Tada et al., Eur J Immunol 2001 : Interestingly, TGF-beta1 enhanced IL-12 p40 production of anti-CD40/IFN-gamma stimulated LC, despite its down-regulatory effect on CD40 expression ... More importantly, it was found that in contrast to TGF-beta, granulocyte/macrophage colony stimulating factor ( GM-CSF ) strikingly inhibits IL-12 production of anti-CD40/IFN -- stimulated LC and that the level of LC IL-12 production is determined by the relative amounts of TGF-beta1 and GM-CSF
Hoffmann et al., J Immunother 2001 (Carcinoma, Squamous Cell...) : While PC upregulated expression of major histocompatibility complex class I/II and costimulatory molecules on the surface of DC, CD40L +/- IFN-gamma increased interleukin (IL)- 12 and to a lesser extent, IL-15 production by DC ... While PC upregulated expression of major histocompatibility complex class I/II and costimulatory molecules on the surface of DC, CD40L +/- IFN-gamma increased interleukin (IL)- 12 and to a lesser extent, IL-15 production by DC ... While PC upregulated expression of major histocompatibility complex class I/II and costimulatory molecules on the surface of DC, CD40L +/- IFN-gamma increased interleukin (IL)- 12 and to a lesser extent, IL-15 production by DC
Utsunomiya et al., J Surg Res 2001 (Burns) : However, IL-12 was not induced by LPS stimulation in TI-mice treated with an inhibitor of PGE ( 2 ) ( indomethacin, 0.1-5 mg/kg ) or an inhibitor of corticosteroid production ( ketoconazole, 10 mg/kg ) ... LPS stimulated IL-12 production was also impaired in normal mice inoculated with burn associated type 2 T cells ... In addition, in the presence of 1 microg/ml LPS , naive macrophages cocultured with burn associated type 2 T cells did not produce IL-12 in their culture fluids, while IL-12 was produced by LPS stimulated naive macrophages that were cocultured with naive splenic CD8 ( + ) T cells
Matera et al., Ann N Y Acad Sci 2000 : By contrast, optimal enhancement of IL-12 induced IFN-gamma release was observed with T cells but not with NK cells
Yokoe et al., Cell Immunol 2001 (Asthma) : Furthermore, intrinsic defects of the CD4 ( + ) T cells, which reduce their IL-12 receptor beta 2 expression in response to IL-12 and/or IL-18 stimulation, are evident and are importantly involved in the Th1/Th2 imbalance of patients with atopic asthma
Maeyama et al., Microbiol Immunol 2001 : Both rCTB and CT suppressed IL-12 secretion induced by interferon gamma (IFN gamma) plus LPS ... Both rCTB and CT suppressed IL-12 secretion induced by interferon gamma (IFN gamma) plus LPS
Smits et al., Eur J Immunol 2001 (Hypersensitivity) : IL-12 is a potent inducer of IFN-gamma production and drives the development of Th1 cells ... These reversed cells are further characterized by a marked IL-12Rbeta2 chain expression and fully restored IL-12-inducible STAT4 activation
Utsunomiya et al., Cytokine 2001 (Burns) : IL-12 was not induced by LPS in sera of normal mice inoculated with burn associated type 2 T cells ( IL-4/IL-10 producing CD8+CD11b+TCRgamma/delta+T cells isolated from spleens of TI-mice ) ... However, IL-12 production was induced by LPS in sera of these mice previously treated with GR or a mixture of monoclonal antibodies ( mAbs ) for type 2 cytokines ... Also, IL-12 production was induced by LPS in TI-mice inoculated with CD4+T cells from spleens of GR-treated normal mice ( GR-CD4+T cells, 5x10 ( 6 ) cells/mouse )
Bullens et al., Scand J Immunol 2001 : CD40L induced IL-12 production is further enhanced by the Th2 cytokines IL-4 and IL-13 ... Inhibition of lipopolysaccharide (LPS) induced IL-12 production by IL-4, IL-13 and IL-10 is supposed to be one such mechanism ... IL-10 potently inhibits IL-12 production ... The increased IL-12 production in the presence of IL-4 and IL-13 is however, not the indirect result of a reduction in IL-10 production, but is most likely owing to a direct effect of IL-4 and IL-13 ... The increased IL-12 production in the presence of IL-4 and IL-13 is however, not the indirect result of a reduction in IL-10 production, but is most likely owing to a direct effect of IL-4 and IL-13 ... The increased IL-12 production in the presence of IL-4 and IL-13 is however, not the indirect result of a reduction in IL-10 production, but is most likely owing to a direct effect of IL-4 and IL-13
Dreher et al., J Leukoc Biol 2001 (Necrosis) : Although all strains stimulated secretion of TNF-alpha and IL-12 strongly, PhoPc induced significantly less IL-10 than the other three strains and as much as 10 times less IL-10 than heat killed PhoPc, suggesting that this mutant suppressed the secretion of IL-10 by the DC
Sakai et al., Blood 2001 (Mycobacterium avium-intracellulare Infection) : Interleukin-12 (IL-12) plays an important role in the production of interferon gamma (IFN-gamma) and is essential for protection against intracellular pathogens such as Mycobacterium and Salmonella
Curti et al., Blood 2001 : IL-11 inhibited IL-12 production by activated CD14 ( + ) monocytes, but not by mature dendritic cells (DCs) stimulated via CD40 ligation
Ortmann et al., J Immunol 2001 (Genetic Predisposition to Disease) : The capacity of NK cells, but not activated T cells, to produce IFN-gamma in response to IL-12 could be partially restored by IL-18
Yap et al., J Immunol 2001 (Acute Disease...) : This study documents a defect in IL-12 dependent IFN-gamma responses in a substrain ( B10.Q-H2- ( q ) /SgJ ) of B10.Q mice that manifests as an acute susceptibility to infection by the intracellular protozoan pathogen, Toxoplasma gondii ... Nonetheless, a delayed IL-12 mediated IFN-gamma response emerges in later stages of acute infection but is unable to prevent host mortality ... IL-18 administration restores, in an IL-12 dependent manner, the early IFN-gamma response and host resistance of B10.Q/J animals ... These in vivo studies indicate that the partially impaired IL-12 responsiveness in B10.Q/J mice can result in defective host resistance and demonstrate a therapeutic function for IL-18 in reversing a genetically based immunodeficiency in IL-12 dependent IFN-gamma production
Azuma et al., Biochem Biophys Res Commun 2001 : Our data clearly demonstrated that dPGJ ( 2 ) inhibits LPS induced IL-10 and IL-12 production by macrophages ... A different agonist of PPARgamma, 13-hydroxyoctadecadienoic acid, similarly inhibited the production of IL-10 and IL-12 in response to LPS ... These results suggest that dPGJ ( 2 ) may inhibit LPS induced IL-10 and IL-12 production by macrophages through PPARgamma
Yao et al., J Interferon Cytokine Res 2001 : We investigated whether the beneficial effects of IFN-beta in MS may involve its role in regulating nitric oxide ( NO ) and interleukin-12 (IL-12) in macrophages, as these immune modulators form part of the innate immune response to intracellular pathogens, such as C. pneumoniae ... In contrast to its effect on inducible NO synthase (iNOS), IFN-beta reduced induction of IL-12/p40 following culture with either elementary body antigens or rMOMP
Hanna et al., Cancer Gene Ther 2001 (Carcinoma, Squamous Cell) : The high level of IL-12 expression led to a 170-fold induction of IFN-gamma expression in serum at day 6 after i.m. electroporation delivery of IL-12 DNA plasmid, which was equal to 1450 pg/mL in the serum
Pekkari et al., Blood 2001 : Trx80 alone induced secretion of IL-12 from CD40 ( + ) monocytes in the PBMC cultures and this effect was enhanced by IL-2
Tang et al., J Allergy Clin Immunol 2001 (Asthma...) : Anti-IL-12 mAb enhanced IL-5 production in the cocultures from atopic nonasthmatic subjects, whereas a dose dependent suppressive effect of recombinant human IL-12 on IL-5 production was seen in atopic asthmatic subjects
Tadokoro et al., Immunol Lett 2001 (Chagas Disease) : Bone marrow derived macrophages grown in GM-CSF or M-CSF differ in their ability to produce IL-12 and to induce IFN-gamma production after stimulation with Trypanosoma cruzi antigens
de Goër de Herve et al., Cytokine 2001 : This IL-12 p40 production was inhibited by anti-CD40 ligand
Lafleur et al., Cancer Res 2001 (Bone Neoplasms...) : Infection of the LM6 cells with an adenoviral vector containing the murine interleukin (IL)-12 gene ( AD : mIL-12 ) or treatment with recombinant murine IL-12 resulted in a dose dependent up-regulation of FAS : The up-regulation of Fas by IL-12 was also demonstrated in human etoposide-resistant MDA-MB-231 breast cancer cells
Rao et al., Br Med Bull 2000 (Bacterial Infections...) : This process is initiated by stimulation with antigen and the cytokines IL-12 and IL-4, respectively, and requires antigen induced transcription factors such as NFAT and cytokine induced transcription factors such as STAT4 , induced by IL-12 , and STAT6, induced by IL-4
Chen et al., J Interferon Cytokine Res 2001 (Empyema, Tuberculous...) : Stimulation with IL-12 plus IL-2, but not with IL-7 plus IL-2, fully restored the IFN-gamma production of mEAL to that of tEAL levels
Gerber et al., J Immunol 2001 : The coligation of FcgammaR along with these stimuli resulted in an anti-inflammatory profile, abrogating IL-12 production and inducing high levels of IL-10
Micallef et al., Int Immunopharmacol 2001 (Melanoma) : The synthesis of IFN-gamma was synergistically enhanced by adding interleukin-18 (IL-18) and this was IL-12 dependent because a neutralizing antibody against IL-12 almost completely inhibited IFN-gamma production while control antibodies ( Abs ) were inactive
Subauste et al., J Infect Dis 2001 (AIDS-Related Opportunistic Infections...) : CD40 ligand trimer (CD40LT) stimulated peripheral blood mononuclear cell ( PBMC ) production of IL-12 in response to Toxoplasma gondii and cytomegalovirus ( CMV ) ... In the presence of CD40LT , PBMC from both HIV infected patients and control subjects produced high levels of IL-12 in response to CMV
de Baey et al., Eur J Immunol 2001 : These DC produce IL-12p75 both in response to LPS and to CD40 ligation
Giacomini et al., J Immunol 2001 : Neutralization of IL-10 restored IL-12 secretion from Mtb infected MDM. In line with these findings, supernatants from Mtb infected MDDC induced IFN-gamma production by T cells and enhanced IL-18R expression, whereas supernatants from MDM failed to do that ... Neutralization of IL-10 restored IL-12 secretion from Mtb infected MDM. In line with these findings, supernatants from Mtb infected MDDC induced IFN-gamma production by T cells and enhanced IL-18R expression, whereas supernatants from MDM failed to do that
Nigou et al., J Immunol 2001 : Here, we report that mannose capped lipoarabinomannans ( ManLAMs ) from Mycobacterium bovis bacillus Calmette-Guérin and Mycobacterium tuberculosis inhibited, in a dose dependent manner, the LPS induced IL-12 production by human dendritic cells ... Mannan, which is a ligand for the mannose receptor ( MR ) as well as an mAb specific for the MR, also inhibited the LPS induced IL-12 production by dendritic cells
Dixon et al., Infect Immun 2001 : Compared to intact bacteria, purified LPS was a very poor inducer of IL-1alpha, IL-6, and TNF-alpha production and induced no detectable IL-12
Martin et al., Infect Immun 2001 : In a coculture system, CT-treated CD4 ( + ) T cells induced significantly less TNF-alpha and IL-12 p70 production by both autologous monocytes and monocyte derived dendritic cells than either LT-IIa- or LT-IIb treated CD4 ( + ) T cells
Felzmann et al., Cancer Lett 2001 : In contrast, the release of IL-12 , an important mediator of anti-tumor immunity, and antigen-specific expansion and IFNgamma secretion of lymphocytes, was strongly triggered only by DCs exposed to CD40L transgenic cells
Keane et al., Am J Physiol Lung Cell Mol Physiol 2001 (Pulmonary Fibrosis) : Interleukin (IL)-12 is a potent inducer of interferon (IFN)-gamma
Kang et al., Immunol Lett 2001 : Parthenolide potently inhibited the LPS induced IL-12 production in a dose dependent manner
Dai et al., Immunology 2001 : These findings demonstrated the ability of GL to enhance LPS induced IL-12 production by peritoneal macrophages, and indicated that the priming effect of GL on IL-12 production was independent of both IFN-gamma and GM-CSF ... These findings demonstrated the ability of GL to enhance LPS induced IL-12 production by peritoneal macrophages, and indicated that the priming effect of GL on IL-12 production was independent of both IFN-gamma and GM-CSF ... These findings demonstrated the ability of GL to enhance LPS induced IL-12 production by peritoneal macrophages, and indicated that the priming effect of GL on IL-12 production was independent of both IFN-gamma and GM-CSF
Gorbachev et al., J Immunol 2001 (Dermatitis, Contact) : In the current report we tested the role of IL-12 on CD8 ( + ) T cell development during sensitization and elicitation of CHS to dinitrofluorobenzene ... Since engagement of CD40 on APC may stimulate IL-12 production, we also tested the role of CD40-CD154 interactions on the development of IFN-gamma producing CD4 ( + ) and CD8 ( + ) T cells following hapten sensitization
Xu et al., J Immunol 2001 (Malaria) : We analyzed the mechanism that causes suppression of IL-12 p40 gene induction during Plasmodium berghei infection ... Therefore, the inhibition of the IL-12 p40 gene induction appeared to be regulated at transcriptional regulation level of the gene
Brewington et al., J Immunol 2001 (Inflammation) : We found that macrophages from IFN-gamma knockout ( IFN-gamma ( -/- ) ) mice exhibit only marginal LPS induced TNF-alpha, IL-12 , and NO responses, all of which can be fully restored in the presence of rIFN-gamma
Németh et al., Biochim Biophys Acta 2001 : The effect of amiloride was post-transcriptional, as IL-12 p40 mRNA levels induced by lipopolysaccharide and interferon-gamma were not affected by this inhibitor ... Furthermore, the inhibitory effect of amiloride on IL-12 p40 production was not a result of interference with the activation of the p38 and p42/44 mitogen activated protein kinases or c-Jun kinase ... Furthermore, the inhibitory effect of amiloride on IL-12 p40 production was not a result of interference with the activation of the p38 and p42/44 mitogen activated protein kinases or c-Jun kinase ... Furthermore, the inhibitory effect of amiloride on IL-12 p40 production was not a result of interference with the activation of the p38 and p42/44 mitogen activated protein kinases or c-Jun kinase
Sheth et al., J Surg Res 2001 : Hsp 27 does not induce secretion of TNF-alpha, IL-10, or IL-12 , whereas LPS does signal IL-12 and TNF-alpha secretion
Amakata et al., Clin Exp Immunol 2001 : IFN-gamma production was completely blocked by an anti-IL-12 antibody, indicating that IFN-gamma secretion was dependent on IL-12 secretion ... These results indicate that the activation of NK cells by DCs is partially mediated by IL-12 secretion, and that direct contact between DCs and NK cells play a major role in this response
Samardzic et al., Clin Exp Immunol 2001 : The inhibitory effect of PTX was specific for IL-18, since LPS induced IL-12 p40 release was not suppressed either in splenocyte cultures or blood of LPS injected animals
Dobashi et al., Clin Exp Immunol 2001 : Regulation of LPS induced IL-12 production by IFN-gamma and IL-4 through intracellular glutathione status in human alveolar macrophages ... Incubation of AM for 2 h with the GSH precursor N-acetylcysteine (NAC) increased the intracellular GSH/GSSG ratio, and enhanced lipopolysaccharide (LPS) induced IL-12 secretion by AM ... Lipopolysaccharide (LPS) induced secretion of IL-12 in AM was enhanced by IFN-gamma but inhibited by IL-4 ... Lipopolysaccharide (LPS) induced secretion of IL-12 in AM was enhanced by IFN-gamma but inhibited by IL-4 ... These results suggest that IFN-gamma and IL-4 oppositely affect the GSH/GSSG balance, which may regulate IL-12 secretion from AM in response to LPS
White et al., J Immunol 2001 : FucT-VII expression was induced by T cell activation, and was enhanced by IL-12 independently of Stat4, whereas C2GlcNAcT-I up-regulation was mediated exclusively by IL-12 , acting through Stat4 ... FucT-VII expression was induced by T cell activation, and was enhanced by IL-12 independently of Stat4, whereas C2GlcNAcT-I up-regulation was mediated exclusively by IL-12 , acting through Stat4
Grohmann et al., J Immunol 2001 : By virtue of their respective actions on CD8 ( - ) and CD8 ( + ) DC, IL-12 and IFN-gamma have functionally opposing effects on peptide presentation by the CD8 ( - ) DC subset, and IFN-gamma activated CD8 ( + ) DC mediate tolerogenic effects that prevail over the adjuvant activity of IL-12 on CD8 ( - ) DC
Kim et al., J Immunol 2001 (Leprosy, Lepromatous...) : Furthermore, IL-12 induced Stat4 phosphorylation and DNA binding in M. leprae activated T cells from tuberculoid but not from lepromatous patients
Stober et al., J Immunol 2001 : IL-12/IL-18 dependent IFN-gamma release by murine dendritic cells ... Endogenous IL-12 p70 produced by DC in response to ligation of CD40 stimulated IFN-gamma release when exogenous IL-18 was supplied
Del Río et al., Infect Immun 2001 (Disease Models, Animal...) : The lack of IL-12 resulted in few infiltrating CD4 ( + ) T cells in the liver relative to the number in WT mice, although the number of CD8 ( + ) T cells was slightly higher
Stuyt et al., Infect Immun 2001 : Staphylococcus spp. induced production of IFN-gamma required the presence of endogenous IL-18, IL-12 , and TNF
Breloer et al., Eur J Immunol 2001 : The presence of Hsp60 induces IFN-gamma and up-regulation of CD69 in T cell/PEC cocultures even in the absence of antigenic peptide and this induction of IFN-gamma is strictly dependent on the ability of the macrophages to produce IL-12 ... The presence of Hsp60 induces IFN-gamma and up-regulation of CD69 in T cell/PEC cocultures even in the absence of antigenic peptide and this induction of IFN-gamma is strictly dependent on the ability of the macrophages to produce IL-12 ... The presence of Hsp60 induces IFN-gamma and up-regulation of CD69 in T cell/PEC cocultures even in the absence of antigenic peptide and this induction of IFN-gamma is strictly dependent on the ability of the macrophages to produce IL-12
Matikainen et al., Eur J Immunol 2001 : During microbial infections macrophage derived IFN-alpha, IL-12 , and IL-18 enhance IFN-gamma production and Th1 response ... We show that Stat4 activation is transient in cells stimulated with IFN-alpha, whereas IL-12 induces more long lasting activation of the transcription factor ... This prolonged activation of IFN-gamma gene by IL-12 may result in more efficient IFN-gamma production compared to that of IFN-alpha
Svensson et al., Microb Pathog 2001 : Furthermore, co-incubating S. typhimurium with Mphi resulted in interleukin (IL)-12p40 , IL-6 and tumor necrosis factor-alpha production as well as iNOS induction while IL-12p70 was not detectable
Nakahira et al., J Immunol 2001 : An absolute requirement for STAT4 and a role for IFN-gamma as an amplifying factor in IL-12 induction of the functional IL-18 receptor complex ... IL-12 induction of an IL-18R complex and IL-18Rbeta mRNA expression was not observed in STAT4-deficient ( STAT4 ( -/- ) ) T cells and was substantially decreased in IFN-gamma ( -/- ) T cells ... These results indicate that STAT4 and IFN-gamma play an indispensable role and a role as an amplifying factor, respectively, in IL-12 induction of the functional IL-18R complex ... These results indicate that STAT4 and IFN-gamma play an indispensable role and a role as an amplifying factor, respectively, in IL-12 induction of the functional IL-18R complex ... These results indicate that STAT4 and IFN-gamma play an indispensable role and a role as an amplifying factor, respectively, in IL-12 induction of the functional IL-18R complex
Weaver et al., J Immunol 2001 : Transfection of NOD DCs with a modified form of IkappaBalpha significantly reduced IL-12 secretion, suggesting that hyperactivation of NF-kappaB was in part responsible for increased IL-12 production
Matasić et al., Croat Med J 2001 : We quantified the effect of drugs on the dendritic cell characteristics, such as stimulation of autologous and allogeneic pan-T cell proliferation, surface marker phenotype, IL-12 p40 subunit secretion, and activation of nuclear transcription factor ( NF)-kappa B
Marriott et al., Clin Exp Immunol 2001 : Inhibition of p38 MAP kinase during cellular activation results in IFN-gamma dependent augmentation of IL-12 production by human monocytes/macrophages
D'Cruz et al., Fertil Steril 2001 : Tyrosine phosphorylation of STAT 1 in capacitated sperm was enhanced by IFN-alpha and IFN-gamma, and that of STAT 4 was enhanced by IL-12
Neighbors et al., J Exp Med 2001 (Listeriosis) : The stimulation of interferon (IFN)-gamma by interleukin (IL)-12 has been shown to provide protection from intracellular pathogens such as Listeria monocytogenes
Poltorak et al., J Immunol 2001 : Because the phosphorylation of STAT4 is a prerequisite for the IL-12 mediated induction of IFN-gamma , its absence in Cr mice is responsible for their defective IFN-gamma response to microorganisms
Ohe et al., Cancer Immunol Immunother 2001 (Fibrosarcoma...) : Furthermore, IL-12 and IL-18 induced by AILb-A inhibited specifically IL-10 and IL-4 production, respectively ... Furthermore, IL-12 and IL-18 induced by AILb-A inhibited specifically IL-10 and IL-4 production, respectively
Zhu et al., Infect Immun 2001 : These Hsp90 inhibitors, however, had no effect on Staphylococcus aureus Cowan strain 1-induced IL-12 production from either the murine spleen cells or macrophages
Yun et al., Infect Immun 2001 (Periodontitis) : Here we demonstrate that in the presence or absence of serum, gingipains were able to hydrolyze IL-12 and reduce the IL-12 induced IFN-gamma production from CD4+ T cells
Iwasaki et al., Eur J Immunol 2001 (Calcium Signaling) : A critical role for IL-12 in CCR5 induction on T cell receptor triggered mouse CD4 ( + ) and CD8 ( + ) T cells ... A critical role for IL-12 in CCR5 induction on T cell receptor triggered mouse CD4 ( + ) and CD8 ( + ) T cells ... A critical role for IL-12 in CCR5 induction on T cell receptor triggered mouse CD4 ( + ) and CD8 ( + ) T cells ... However, TCR triggered T cells expressed IL-12 receptor, and stimulation with recombinant IL-12 resulted in high levels of CCR5 expression on both CD4 ( + ) and CD8 ( + ) T cells ... Stimulation of these CCR5 positive T cells with the relevant chemokine MIP-1 alpha elicited Ca ( 2+ ) influx, showing that IL-12 induced CCR5 is functional ... These results indicate a critical role for IL-12 in the induction of CCR5 on TCR triggered T cells
Lissoni et al., J Biol Regul Homeost Agents 2001 (Neoplasms...) : Stimulation of IL-12 secretion by GM-CSF in advanced cancer patients ... Unfortunately, at present there are only few and controversial results on GM-CSF effects on IL-12 secretion in cancer patients ... This preliminary study seems to show that GM-CSF may acutely stimulate IL-12 secretion in cancer patients ... Further studies are required to evaluate the effects of chronic GM-CSF administration, and the impact of GM-CSF induced secretion of IL-12 on the efficacy of the immunotherapies of cancer with cytokines, such as IL-2
Boneberg et al., J Pharmacol Exp Ther 2001 : However, the lipopolysaccharide (LPS) induced release of tumor necrosis factor (TNF)-alpha was increased, and the secretion of interleukin (IL)-10 was reduced by Lektinol at a mistletoe lectin-1 (ML-1) concentration of 0.5 to 5 ng/ml, whereas the LPS induced secretion of IL-1 beta, IL-6, IL-12 , and interferon-gamma was not affected
Panerai et al., Diabetologia 2001 (Diabetes Mellitus, Type 1...) : Anti-CD3 and/or lipopolysaccharide (LPS) induced blood levels of interferon (IFN)-gamma, interleukin (IL)-4, IL-10, IL-12 and tumour necrosis factor (TNF)-alpha were measured by ELISA in 10 week-old female NOD mice treated for 6 consecutive weeks with either MFP14 or PBS
Kitagawa et al., J Rheumatol 2001 (Arthritis, Rheumatoid) : To determine the role of interferon-gamma (IFN-gamma) in CD40-CD154 dependent production of interleukin 12 (IL-12) by synovial cells of patients with rheumatoid arthritis ( RA ) ... To determine the role of interferon-gamma (IFN-gamma) in CD40-CD154 dependent production of interleukin 12 (IL-12) by synovial cells of patients with rheumatoid arthritis ( RA ) ... To determine the role of interferon-gamma (IFN-gamma) in CD40-CD154 dependent production of interleukin 12 (IL-12) by synovial cells of patients with rheumatoid arthritis ( RA ) ... The effects of IFN-gamma and soluble CD154 ( sCD154 ) on IL-12 production by RA synovial cells were determined by ELISA ... The effects of IFN-gamma and soluble CD154 ( sCD154 ) on IL-12 production by RA synovial cells were determined by ELISA ... IFN-gamma enhanced IL-12 production via both CD40-CD154 dependent and independent pathways in RA synovium
Tanaka et al., J Rheumatol 2001 (Arthritis, Rheumatoid...) : In the presence of IL-12 , however, IL-18 could synergistically stimulate IFN-gamma production by RA synovial tissue cells
Becker et al., J Immunol 2001 : Finally, we observed that IL-4 mediated repression of IL-12 p40 promoter activity is critically dependent on an intact GA-12 sequence
Matsumoto et al., Int Immunopharmacol 2001 (Lung Neoplasms) : ( 6 ) Male patients showed more severe suppression of IL-12/IL-18 mediated IFNgamma production than female patients
Galetto et al., J Surg Res 2001 (Adenocarcinoma...) : Activation of IFN-gamma release was accompanied by tumor cytotoxicity and both responses were enhanced by IL-12 , indicating the functional integrity of patients ' lymphocytes
Canning et al., Eur J Endocrinol 2001 : SAC/interferon-gamma induced IL-12 production was clearly upregulated in both types of DC relative to those of the original monocytes, and TGF-beta production was downregulated
Yoshida et al., Cell Immunol 2001 : IL-18 in the same concentration range stimulated the production of IL-12, TNF-alpha, and IFN-gamma in culture of PBMC ; however, IL-18 induced expression of ICAM-1 in monocytes was not inhibited by anti-IL-12 , anti-TNF-alpha, or anti-IFN-gamma Ab, suggesting the independence of the upregulating effect of IL-18 on endogenous IL-12, TNF-alpha, and IFN-gamma production ... IL-18 in the same concentration range stimulated the production of IL-12 , TNF-alpha, and IFN-gamma in culture of PBMC ; however, IL-18 induced expression of ICAM-1 in monocytes was not inhibited by anti-IL-12, anti-TNF-alpha, or anti-IFN-gamma Ab, suggesting the independence of the upregulating effect of IL-18 on endogenous IL-12, TNF-alpha, and IFN-gamma production ... On the other hand, anti-ICAM-1 and anti-LFA-1 Abs inhibited IL-18 induced production of three cytokines, IL-12 , IFN-gamma, and TNF-alpha, by 60 and 40 %, respectively ... On the other hand, anti-ICAM-1 and anti-LFA-1 Abs inhibited IL-18 induced production of three cytokines, IL-12 , IFN-gamma, and TNF-alpha, by 60 and 40 %, respectively
Demeure et al., Eur J Cancer 2001 (Carcinoma, Renal Cell...) : In contrast, MHC class II engagement by LAG-3Ig was found to enhance the capacity of immature dendritic cells to stimulate naive T cell proliferation and IL-12 dependent IFN-gamma production by T cells in vitro
Mukai et al., J Leukoc Biol 2001 : These results indicate that IL-12 plays a critical role in CCR5 expression on Th1 cells and consequently contributes to CCR5 mediated activation of LFA-1 molecules
Ethuin et al., J Leukoc Biol 2001 : Because IL-8 is a strong chemokine for polymorphonuclear neutrophils ( PMNs ), we investigated the effect of IL-12 on PMN IL-8 production
Shikano et al., Clin Exp Allergy 2001 (Hypersensitivity, Immediate) : IFN-gamma production in response to IL-18 or IL-12 stimulation by peripheral blood mononuclear cells of atopic patients ... Several studies have shown that interleukin (IL)-4 and interferon-gamma (IFN-gamma) are important for the regulation of immunoglobulin E ( IgE ) production and that IL-18 and IL-12 induce IFN-gamma ... Several studies have shown that interleukin (IL)-4 and interferon-gamma (IFN-gamma) are important for the regulation of immunoglobulin E ( IgE ) production and that IL-18 and IL-12 induce IFN-gamma ... IFN-gamma production in response to IL-18 or IL-12 stimulation was investigated in peripheral blood mononuclear cells ( PBMCs ) of atopic patients with various levels of serum IgE
Panther et al., FASEB J 2001 : However, in LPS differentiated DCs, adenosine and an A ( 2a ) receptor agonist stimulated adenylate cyclase activity, enhanced intracellular cAMP levels, and inhibited interleukin 12 (IL-12) production
Bleharski et al., J Immunol 2001 : Functionally, engagement of SLAM enhances DC production of IL-12 and IL-8, while having no effect on production of IL-10
Koizumi et al., Arch Dermatol Res 2001 : Human interleukin-18 (IL-18) enhances IL-12 mediated IFN-gamma production by lymphocytes and Fas/perforin mediated cytolysis by NK cells
Qadir et al., Am J Physiol Gastrointest Liver Physiol 2001 (Granuloma, Foreign-Body...) : IL-12 , IL-18, and soluble egg antigen stimulated IFN-gamma release from C57BL/6 IL-4-/- and WT mice ... IFN-gamma production in C57 IL-4-/- and WT granulomas was IL-12 dependent , because IL-12 blockade partly abrogated IFN-gamma secretion after stimulation
Siegmund et al., Am J Physiol Regul Integr Comp Physiol 2001 (Colitis...) : The stimulation of lamina propria mononuclear cells by IL-18 and IL-12 resulted in a synergistic increase in IFN-gamma synthesis
Neumann et al., J Interferon Cytokine Res 2001 : First, IL-12 induced expression of the IL-18 receptor complex was described
D'Agostino et al., Int Immunopharmacol 2001 : On the contrary, IL-12 mRNA expression was significantly increased by CMT-1 and CMT-8 with LPS activation
Caron et al., J Immunol 2001 (Hypersensitivity) : We report here that histamine inhibits LPS induced IL-12 production and polarizes uncommitted maturing DC into effector DC2 ... DC matured in the presence of histamine fail to produce IL-12 upon subsequent stimulation and prime Th2 responses, even in presence of IFN-gamma , a potent DC1 driving factor
Frucht et al., Trends Immunol 2001 : As in lymphoid cells, IL-12 induced IFN-gamma production in APCs requires signal transducer and activator of transcription 4 ( STAT4 ), although the precise molecular events that govern the transcription of the gene encoding IFN-gamma are enigmatic still
Viganò et al., Mol Hum Reprod 2001 : Production of IL-10 by the uNK cells was enhanced by both IL-2 and IL-12
Peacock et al., Immunology 2001 (Herpesviridae Infections...) : While the lack of IL-10 results in increased IL-12 expression and a lower viral burden, IL-10-/- mice also experience an increased leukocytosis
Loza et al., Nat Immunol 2001 : In human NK cell clones, polyclonal CD3-CD161+CD56- cells and peripheral lymphocytes, IL-4 induced the proliferation of both IL-13+ NK and T cells, whereas IL-12 allowed a proliferation independent accumulation of IFN-gamma+ cells
Wang et al., Biochem Biophys Res Commun 2001 (Inflammation) : We investigated the effect of heat shock response on LPS induced production of IL-12 and IL-10, which are two important cytokines playing contradictory roles in regulation of immune response, by murine peritoneal macrophages ... The data showed that induction of heat shock response strongly suppressed LPS induced production of IL-12 while augmented that of IL-10, suggesting the pleiotropic effects of heat shock response on immune regulatory gene expression
Lim et al., J Immunol 2001 : The data show IL-12/STAT4 signaling is necessary for induction of C2GnT , but not Fuc-TVII mRNA, and that STAT4 ( -/- ) Th1 cells do not traffic normally to sites of inflammation in vivo, do not interact with P-selectin, and exhibit a partial reduction of E-selectin interactions under shear stress in vitro
Qi et al., J Immunol 2001 (Disease Susceptibility...) : Infection with amastigotes fails to induce CD40 dependent IL-12 production, but rather potentiates IL-4 production in BALB/c DCs
Gosset et al., Eur J Immunol 2001 : Moreover, activation of PPARgamma resulted in a dramatic decreased secretion of the Th1 promoting factor IL-12 in LPS- and CD40L stimulated cells ( by 47 % and 62 % ), while the production of IL-1beta, TNF-alpha, IL-6 and IL-10 was unaffected
Nomura et al., Exp Hematol 2001 : After stimulation with or without IL-18 in the presence of IL-12 for 48 hours ( NK cells ) or 72 hours ( T cells or CD45RA ( + ) T cells ), IFN-gamma concentration was measured in each subset ... Although IL-18 induced significant IFN-gamma production from both CB and adult PB T cells in the presence of IL-12 , the IFN-gamma levels from CB T cells were lower than those from adult PB T cells
Grumont et al., J Exp Med 2001 : Here we show that in CD8 ( + ) dendritic cells, major producers of IL-12 p70 , the Rel/nuclear factor (NF)-kappaB signaling pathway is necessary for the induction of IL-12 in response to microbial stimuli ... This data establishes the IL-12 p35 gene as a new target of c-Rel and shows that the regulation of IL-12 p70 expression at the transcriptional level by Rel/NF-kappaB is controlled through both the p35 and p40 genes in a cell type-specific fashion ... This data establishes the IL-12 p35 gene as a new target of c-Rel and shows that the regulation of IL-12 p70 expression at the transcriptional level by Rel/NF-kappaB is controlled through both the p35 and p40 genes in a cell type-specific fashion
Vaidyanathan et al., Cytokine 2001 : Despite comparable levels of total p35 mRNA, LPS induced p35 exon 1 transcripts led to significantly more bioactive IL-12 from SAC than IFN-gamma/CD40 induced exon 1a/exon 2 transcripts as measured by ELISA
Pulendran et al., J Immunol 2001 : Consistent with these results, E. coli LPS induced IL-12 ( p70 ) in the CD8alpha ( + ) dendritic cell ( DC ) subset, while P. gingivalis LPS did not
Buentke et al., Clin Exp Allergy 2001 : The CD40L stimulated IL12p70 production by MDDCs was decreased in the presence of M. furfur ( P < 0.05 )
Padigel et al., J Immunol 2001 (Leishmaniasis, Cutaneous) : At wk 9 of infection, we detected similar levels of IL-4, IFN-gamma , IL-12p40, and IL-12Rbeta2 mRNA in draining lymph nodes of healing C57BL/6, CD28 ( -/- ), and CD40L ( -/- ) CD28 ( -/- ) mice, whereas CD40L ( -/- ) mice had increased mRNA levels for IL-4 but reduced levels for IFN-gamma, IL-12p40 , and IL-12Rbeta2 ... At wk 9 of infection, we detected similar levels of IL-4, IFN-gamma, IL-12p40 , and IL-12Rbeta2 mRNA in draining lymph nodes of healing C57BL/6, CD28 ( -/- ), and CD40L ( -/- ) CD28 ( -/- ) mice, whereas CD40L ( -/- ) mice had increased mRNA levels for IL-4 but reduced levels for IFN-gamma, IL-12p40, and IL-12Rbeta2 ... At wk 9 of infection, we detected similar levels of IL-4, IFN-gamma, IL-12p40 , and IL-12Rbeta2 mRNA in draining lymph nodes of healing C57BL/6, CD28 ( -/- ), and CD40L ( -/- ) CD28 ( -/- ) mice, whereas CD40L ( -/- ) mice had increased mRNA levels for IL-4 but reduced levels for IFN-gamma, IL-12p40 , and IL-12Rbeta2 ... At wk 9 of infection, we detected similar levels of IL-4 , IFN-gamma, IL-12p40, and IL-12Rbeta2 mRNA in draining lymph nodes of healing C57BL/6, CD28 ( -/- ), and CD40L ( -/- ) CD28 ( -/- ) mice, whereas CD40L ( -/- ) mice had increased mRNA levels for IL-4 but reduced levels for IFN-gamma, IL-12p40 , and IL-12Rbeta2
Ohteki et al., Nat Immunol 2001 : Activation of dendritic cells (DCs) and macrophages by infectious agents leads to secretion of interleukin 12 (IL-12) , which subsequently induces interferon-gamma (IFN-gamma) production by multiple cell types that include DCs and macrophages
Pérez-Santos et al., Parasite Immunol 2001 (Disease Models, Animal...) : In vitro administration of indomethacin (INDO), a specific inhibitor of PGs synthesis, reduced PGs production at normal levels, and increased IL-12 , IFN-gamma, and NO production in infected BALB/c mice
Zhang et al., J Interferon Cytokine Res 2001 : Potentially, IL-18 and IL-12 can augment IFN-gamma production and reverse CD8 ( + ) CTL deficiency in aging, independently or synergistically
Chakir et al., Cell Immunol 2001 : Production of IFN-gamma in response to either IL-12 or IL-18 was dependent upon costimulation with either IL-2 or IL-15 ... DX5+ splenocytes showed a rapid ( 6 h ) and sustained ( 6-72 h ) accumulation of IFN-gamma transcripts followed by a delayed ( 12-24 h ) up-regulation of IL-10 or IL-13 expression in response to IL-2 + IL-12 or IL-2 + IL-18, respectively ... DX5+ splenocytes showed a rapid ( 6 h ) and sustained ( 6-72 h ) accumulation of IFN-gamma transcripts followed by a delayed ( 12-24 h ) up-regulation of IL-10 or IL-13 expression in response to IL-2 + IL-12 or IL-2 + IL-18, respectively
Galvão da Silva et al., Immunology 2001 (Acute Disease...) : Enhancement of proliferation promoted by IL-12 occurred in the presence of neutralizing anti-interleukin-2 (IL-2) antibody, suggesting that this activity of IL-12 was partly independent of endogenous IL-2
Hussien et al., J Neuroimmunol 2001 (Multiple Sclerosis, Chronic Progressive...) : IFN-beta inhibits IL-12 production by DCs over unknown mechanisms ... IFN-beta also reduced IL-12 , but increased IL-10 production by DCs from both MS patients and healthy controls
Rhee et al., Clin Exp Immunol 2001 (Malaria, Falciparum) : We find that cells from naive donors produce moderate amounts of IFN-gamma in response to PfSE and that IFN-gamma production is strongly IL-12 dependent
Iwasaki et al., J Immunol 2001 : A mandatory role for STAT4 in IL-12 induction of mouse T cell CCR5 ... A mandatory role for STAT4 in IL-12 induction of mouse T cell CCR5 ... In contrast, TCR triggered STAT4 ( -/- ) T cells failed to express CCR5 in response to IL-12
Moore et al., J Virol 2002 : IL-12 induced the greatest increase in IFN-gamma and immunoglobulin G responses to Nef, and GM-CSF induced the strongest IFN-gamma and CTL responses to Env ... IL-12 induced the greatest increase in IFN-gamma and immunoglobulin G responses to Nef, and GM-CSF induced the strongest IFN-gamma and CTL responses to Env
Du et al., Experimental biology and medicine (Maywood, N.J.) 2001 : Further studies showed that DHEA enhanced IL-4 , but inhibited IL-12 mediated T cell proliferation and IL-12 production in antigen presenting cells (APCs)
Salmon et al., Eur J Immunol 2001 : The enhancing effect of SB 203580 on the production of IL-12 by peritoneal exudate macrophages stimulated by LPS and IFN-gamma was dose dependent ( EC ( 50 ) 0.3 microM ), was only seen at lower concentrations of IFN-gamma and was due , at least in part, to a dose dependent ( IC ( 50 ) 0.3 microM ) inhibition of the production of IL-10
Taoufik et al., Eur J Immunol 2001 : IL-12 induced tyrosine phosphorylation and nuclear translocation of STAT4 ... IL-12p70 and, more effectively, the combination of IL-12p70 and IFN-gamma, induced IL-12p40 mRNA expression and bioactive IL-12p70 production ... IL-12p70 and, more effectively, the combination of IL-12p70 and IFN-gamma, induced IL-12p40 mRNA expression and bioactive IL-12p70 production
Thornton et al., Eur J Immunol 2001 (Autoimmune Diseases) : Furthermore, IFN-gamma secretion in response to IL-2 administration was not affected by the absence of IL-12 , the pivotal cytokine for determination of Th1 responses
Zou et al., Eur J Immunol 2001 (Adenovirus Infections, Human) : PDC type I interferons inhibited MDC differentiation and function ( reduced IL-12 secretion, IFN-gamma induction , MLR and CD40 expression, and increased CD1a ( + ) CD14 ( + ) cells )
Reader et al., Prostate 2001 (Prostatic Neoplasms) : The synthesis of IL-12 , GM-CSF, and TNF-alpha by LNCaP cells in response to MCC was also determined ... Surprisingly, MCC also directly induced the synthesis of IL-12 and GM-CSF, but not TNF-alpha, by LNCaP cells
Afanasyeva et al., Circulation 2001 (Autoimmune Diseases...) : We questioned whether IFN-gamma is required for the disease promoting activity of IL-12
Sweet et al., J Immunol 2002 : CSF-1 pretreatment enhanced IL-6, IL-12 , and TNF-alpha production in response to LPS but suppressed the same response to CpG DNA
Takahashi et al., J Pharmacol Exp Ther 2002 : In the present study, we demonstrate that histamine inhibited the ICAM-1 expression in monocytes induced by IL-18 using flow cytometry and that the responses of IL-12 , IFN-gamma, and TNF-alpha induced by IL-18 were concentration dependently inhibited by coexisting histamine, whereas IL-18 inhibited IL-10 production was reversed by the same concentrations of histamine
Ansell et al., Blood 2002 (Lymphoma, B-Cell) : Interleukin-12 (IL-12) facilitates cytolytic T-cell responses, enhances the lytic activity of natural killer ( NK ) cells, and induces the secretion of interferon gamma (IFN-gamma) by both T and NK cells
Märten et al., J Immunother 2001 : Interactions between DCs and CIK cells caused changes in the surface molecule expression of both populations, led to an increase of IL-12 secretion, and rendered an improved cytotoxic activity
Takeda et al., Hum Cell 2001 (Neoplasm Metastasis...) : Consisting with this finding, IL-12 and NKT cell specific ligand, alpha-Galactosylceramide ( alpha-GalCer ), induced TRAIL mediated cytotoxcity and anti-tumor effect, and which was mediated by TRAIL expressed on IFN-gamma activated NK cells
Mendel et al., J Neuroimmunol 2002 (Disease Models, Animal...) : We demonstrate that the proliferative response and IFN-gamma production by a fully differentiated T cell line specific for myelin oligodendrocyte glycoprotein are completely independent of IL-12 and CD40/CD40L interactions
Luft et al., J Immunol 2002 : CD40L potently induces dendritic cell ( DC ) maturation and IL-12p70 secretion and plays a critical role during T cell priming in the lymph nodes
Ismaili et al., J Immunol 2002 (Calcium Signaling) : However, lower levels of IL-12 were induced by MPL when compared with lipopolysaccharide
Zhang et al., Zhonghua Jie He He Hu Xi Za Zhi 2000 (Tuberculosis) : IL-12 induced IFN-gamma production, promoted development of Th1 cell responses, modulated Th1 and Th2 cytokine balance, suggesting that IL-12 enhance the host defense against the infection
Böcker et al., Eur Cytokine Netw 2001 : Secretion of IL-18 was independent of functional activity of IL-10, IL-12 or IFN-gamma
Wagner et al., J Leukoc Biol 2002 (Tuberculosis) : Stimulation of macrophages with IFN-gamma prior to infection with M. avium resulted in greater production of IL-12 compared with unstimulated macrophages
Yanagawa et al., J Leukoc Biol 2002 : These findings suggest that TNF-alpha induced ERK activation negatively controls maturation and IL-12 production in murine DC ... These findings suggest that TNF-alpha induced ERK activation negatively controls maturation and IL-12 production in murine DC
Camoglio et al., Eur J Immunol 2002 (Crohn Disease...) : These results demonstrate that IL-12p40 , in contrast to IL-12p70, inhibits TNBS induced colitis and IL-18 expression independent of IFN-gamma
Schwacha et al., Am J Physiol Cell Physiol 2002 (Bacterial Infections...) : Thus elevated Mphi COX-2 activity, independent of IL-10, suppresses Mphi IL-12 production after thermal injury and may play an important role in the observed immunosuppression under such conditions
Bolacchi et al., Cytokine 2001 : Here we show that CD40L ( ligand for CD40 ) failed to induce the production of tumour necrosis factor alpha (TNF-alpha), interleukin (IL-)-1 beta, IL-10 and IL-12 in macrophages matured in vitro in the absence of growth factors or in the presence of macrophage colony stimulating factor ( M-CSF )
Li et al., Cytokine 2001 : We report the effect of heat shock on lipopolysaccharide (LPS) induced interleukin 12 (IL-12) expression
Reider et al., J Allergy Clin Immunol 2002 (Hypersensitivity) : Production of IL-4, IL-5, IL-10, IL-12 , and IL-13 in response to CD40 ligation was measured with ELISA
Nakahira et al., J Immunol 2002 : Synergy of IL-12 and IL-18 for IFN-gamma gene expression : IL-12 induced STAT4 contributes to IFN-gamma promoter activation by up-regulating the binding activity of IL-18 induced activator protein 1 ... IL-12 and IL-18 synergistically enhance IFN-gamma mRNA transcription by activating STAT4 and AP-1, respectively ... An increase in c-Jun, a component of AP-1, in the nuclear compartment was elicited by stimulation with either IL-12 or IL-18, but accumulation of serine phosphorylated c-Jun was induced only by IL-18 capable of activating c-Jun N-terminal kinase ... An increase in c-Jun, a component of AP-1, in the nuclear compartment was elicited by stimulation with either IL-12 or IL-18, but accumulation of serine phosphorylated c-Jun was induced only by IL-18 capable of activating c-Jun N-terminal kinase ... These results show that STAT4 up-regulates AP-1 mediated IFN-gamma promoter activation without directly binding to the promoter sequence, providing a mechanistic explanation for IL-12/IL-18 induced synergistic enhancement of IFN-gamma gene expression
Majumder et al., Int J Cancer 2002 (Carcinoma, Ehrlich Tumor...) : PRL induced release of IL-12 from EMs also, which suggested that the hormone might drive the TH1 response through IL-12
Takeuchi et al., Lung Cancer 2002 (Carcinoma, Small Cell...) : IL-12 induced production of IL-10 and interferon-gamma by mononuclear cells in lung cancer associated malignant pleural effusions ... IL-12 induced production of IL-10 and interferon-gamma by mononuclear cells in lung cancer associated malignant pleural effusions ... Interleukin (IL)-12 enhances natural killer ( NK ) activity and induces interferon gamma (IFN-gamma) production ... Recently, it was shown that IL-12 induces IL-10 production by human T cells and NK cells, as a negative feedback for IL-12 induced immune responses ... In the present study, in order to investigate the functions of host immune cells existing in contact with cancer cells, we examined the effect of IL-12 on the induction of non-major histocompatibility complex ( MHC ) -restricted killer activity and of IFN-gamma and IL-10 production by pleural and peripheral blood mononuclear cells ( MNC ), isolated from 40 lung cancer patients and 20 control subjects ... In the present study, in order to investigate the functions of host immune cells existing in contact with cancer cells, we examined the effect of IL-12 on the induction of non-major histocompatibility complex ( MHC ) -restricted killer activity and of IFN-gamma and IL-10 production by pleural and peripheral blood mononuclear cells ( MNC ), isolated from 40 lung cancer patients and 20 control subjects
Bonder et al., Growth Factors 2001 : While IL-4 suppresses LPS induced IL-1beta, IL-12 , IL-10 and TNFalpha production by monocytes, IL-4 suppresses only IL-1beta and IL-12 production by MDMac
Yang et al., J Clin Immunol 2001 : However, when purified T cells instead of unfractionated PBL were stimulated with anti-CD3/CD28 and then exposed to IL-12 or IFN-alpha, CCR5 expression was induced by IL-12 but not by IFN-alpha ... However, when purified T cells instead of unfractionated PBL were stimulated with anti-CD3/CD28 and then exposed to IL-12 or IFN-alpha, CCR5 expression was induced by IL-12 but not by IFN-alpha
Loza et al., Eur J Immunol 2002 : IL-4 induced preferential proliferation of the pre existing peripheral IL-13+ cells, whereas IL-12 had minimal effect on proliferation of the IFN-gamma+ NK cells ... The IL-13+ cells ( CD161+ only ) are phenotypically distinct from the IFN-gamma+ ones ( CD56+ ) before and after culture under any condition analyzed, and produce IL-13 in response to NK-sensitive target cells and PMA+Ca ( 2+ ) ionophore, whereas also FcgammaRIIIA and IL-2+IL-12 stimulate IFN-gamma production
Hazlett et al., Invest Ophthalmol Vis Sci 2002 (Corneal Ulcer...) : IFN-gamma is the predominant cytokine induced by IL-12, and positive regulation of IL-12 by IFN-gamma , if unchecked, leads to excessive cytokine production and toxicity ... IL-12 knockout mice, histopathology, RT/PCR and ELISA analyses, immunocytochemistry, and quantitation of viable bacteria in cornea were used to examine the role of IL-12 in IFN-gamma production and the susceptibility phenotype ... To directly test the effect of IL-12 on IFN-gamma production, IL-12 knockout and wild-type C57BL/6 mice were used
Säemann et al., J Leukoc Biol 2002 : In particular, n-butyrate prevents homotypic DC clustering, inhibits IL-12 while sparing IL-10 production, and at the molecular level, blocks NF-kappa B translocation
Utsugi et al., J Leukoc Biol 2002 : We examined whether changes in intracellular reduced ( GSH ) or oxidized ( GSSG ) glutathione of human monocytes regulate lipopolysaccharide (LPS) induced IL-12 production and defined the molecular mechanism that underlies glutathione redox regulation ... LPS induced IL-12 production and p38 mitogen activated protein ( MAP ) kinase activation were enhanced by GSH-OEt but suppressed by DEM. Selective p38 inhibitors showed that p38 promoted GSH-OEt enhanced IL-12 production ... LPS induced IL-12 production and p38 mitogen activated protein ( MAP ) kinase activation were enhanced by GSH-OEt but suppressed by DEM. Selective p38 inhibitors showed that p38 promoted GSH-OEt enhanced IL-12 production ... These findings reveal that glutathione redox regulates LPS induced IL-12 production from monocytes through p38 MAP kinase activation and that the priming effect of IFN-gamma on IL-12 production is partly a result of the glutathione redox balance ... These findings reveal that glutathione redox regulates LPS induced IL-12 production from monocytes through p38 MAP kinase activation and that the priming effect of IFN-gamma on IL-12 production is partly a result of the glutathione redox balance
Burkart et al., J Immunol 2002 : Pre-exposure to CTB also suppresses LPS induced TNF-alpha and IL-12 ( p70 ) formation in human PBMC
Hermann et al., Eur J Immunol 2002 : While the maximal achievable monokine release in response to LTA was comparable to LPS, all LTA induced significantly less IL-12 and IFN-gamma ... IL-12 substitution increased LTA-inducible IFN-gamma release up to 180-fold, suggesting a critical role of poor LTA-inducible IL-12 for IFN-gamma formation ... In conclusion, LTA compared to LPS, are weak inducers of IL-12 and subsequent IFN-gamma formation which might explain their lower toxicity in vivo
Ho et al., Scand J Rheumatol 2001 : Aspirin, at therapeutic concentrations, also decreased LPS induced IL-12 and IL-10 production
Kobashi et al., Anticancer Res 2001 (Neoplasms) : We also measured IL-18 stimulated IL-12 production in healthy volunteers and null response was observed in cancer bearing patients
Koguchi et al., Infect Immun 2002 (AIDS-Related Opportunistic Infections) : We investigated the role of osteopontin (OPN) in interleukin-12 (IL-12) production from peripheral blood mononuclear cells ( PBMCs ) stimulated with Penicillium marneffei ... Furthermore, native OPN induced a profound level of synthesis of IL-12 from noninfected PBMCs ... Our results demonstrated that OPN secreted from monocytes is involved in the production of IL-12 from PBMCs after stimulation with P. marneffei and that OPN production is regulated by GM-CSF
Nomura et al., Infect Immun 2002 : These results clearly indicated that LLO, a well-known virulence factor of L. monocytogenes, is capable of inducing IFN-gamma from NK cells through induction of IL-12 and IL-18 from macrophages
Shaw et al., Infect Immun 2002 (Chlamydia Infections) : Immunity generated by infection or ex vivo antigen pulsed DC correlates with a chlamydia-specific interleukin 12 (IL-12) dependent CD4 ( + ) Th1 immune response
Moll et al., Infect Immun 2002 : Increased interleukin 4 (IL-4) receptor expression and IL-4 induced decrease in IL-12 production by Langerhans cells infected with Leishmania major
Veenstra et al., J Immunol 2002 (Neoplasms) : IL-12 induces monocyte IL-18 binding protein expression via IFN-gamma ... In this report we show that IL-18BPa , the IL-18BP isoform with the highest affinity for IL-18, was strongly induced by IL-12 in human PBMC ... In this report we show that IL-18BPa , the IL-18BP isoform with the highest affinity for IL-18, was strongly induced by IL-12 in human PBMC ... Although monocytes were found to be the primary source of IL-18BPa, the induction of IL-18BPa by IL-12 was mediated through IFN-gamma derived predominantly from NK cells ... Although monocytes were found to be the primary source of IL-18BPa, the induction of IL-18BPa by IL-12 was mediated through IFN-gamma derived predominantly from NK cells
Major et al., J Immunol 2002 : Although well recognized for its anti-inflammatory effect on gene expression in stimulated monocytes and macrophages, IL-4 is a pleiotropic cytokine that has also been shown to enhance TNF-alpha and IL-12 production in response to stimulation with LPS
Zhang et al., J Immunol 2002 (Encephalomyelitis, Autoimmune, Experimental) : IL-12 blocked the suppressive effect of i.v. tolerance on the proliferative response to MBP1-11 and MBP1-11 induced production of IL-12 and IFN-gamma
Ouaaz et al., Immunity 2002 : In contrast, DCs from p50 ( -/- ) cRel ( -/- ) mice developed normally, but CD40L- and TRANCE induced survival and IL-12 production was abolished
Milano et al., Clin Exp Immunol 2002 (Leishmaniasis, Visceral) : Since IL-15 , in the presence of anti-IL-4 MoAb, caused a further increase in IL-12 production and led to a significant production of IFN-gamma, one of its indirect effects on Th1 cell activation could be due to the latter 's effect on Th2 cytokines such as IL-4
Shimoda et al., Blood 2002 : Tyk2 is activated in response to interleukin-12 (IL-12) and is essential for IL-12 induced T-cell function, including interferon-gamma (IFN-gamma) production and Th1 cell differentiation
Gumperz et al., J Exp Med 2002 : In contrast, CD4 ( + ) CD1d restricted NKT cells potently produced both Th1 and Th2 cytokines, up-regulated perforin in response to stimulation by phorbol myristate acetate and ionomycin but not IL-2 or IL-12 , and could be induced to express CD95L
Tarkowski et al., Clin Exp Immunol 2002 : The effect of IL-18 on IL-12 induced CD30 expression and IL-4 and IFN-gamma production by allergen and PPD specific T cells ... The effect of IL-18 on IL-12 induced CD30 expression and IL-4 and IFN-gamma production by allergen and PPD specific T cells ... The effect of IL-18 on IL-12 induced CD30 expression and IL-4 and IFN-gamma production by allergen and PPD specific T cells ... The aim of our study was to investigate whether IL-18 affects the IL-12 induced CD30 expression and cytokine production by allergen or PPD specific T cells
Yoshida et al., Naunyn Schmiedebergs Arch Pharmacol 2002 : Essential role of ICAM-1/LFA-1 interaction in synergistic effect of IL-18 and IL-12 on IFN-gamma production in human PBMC ... The effect of IL-12 on ICAM-1 expression on monocytes was marginal even at the highest concentration ( 100 ng/ml ) ... However, in the presence of IL-12 ( 100 ng/ml ), the expression of ICAM-1 induced by IL-18 was significantly enhanced as compared with that obtained by IL-18 alone ... In addition to the expression of ICAM-1 on monocytes, IFN-gamma production was synergistically stimulated by IL-18 and IL-12 ... These results as a whole indicated that synergistic effect of IL-18 and IL-12 on IFN-gamma production in human PBMC is ascribed to the synergism of the effect of two cytokines on ICAM-1 expression on monocytes and that the subsequent ICAM-1/LFA-1 interaction plays an important role in the enhanced production of IFN-gamma
Ding et al., Cytokine 2001 : LPS induced increases in production of tumour necrosis factor alpha (TNF-alpha), interleukin 1beta (IL-1beta), IL-10 and IL-12 were significantly inhibited by the over-expression of HSP-70
Mason et al., J Immunol 2002 (Inflammation...) : Recent studies have shown that c-Rel, a member of the NF-kappaB family of transcription factors, is essential for LPS induced IL-12p40 production by macrophages ... Recent studies have shown that c-Rel , a member of the NF-kappaB family of transcription factors, is essential for LPS induced IL-12p40 production by macrophages ... In this study, we demonstrate that c-Rel is also required for IL-12p40 production by macrophages in response to Corynebacterium parvum, CpG oligodeoxynucleotides, anti-CD40 and low molecular weight hyaluronic acid ... Furthermore, c-Rel was not required for IL-12p40 production by macrophages or dendritic cells in response to soluble Toxoplasma Ag, and neutrophils from c-Rel ( -/- ) mice contain normal amounts of preformed IL-12p40
Giambartolomei et al., Infect Immun 2002 : To understand the mechanism ( s ) implicated in the regulation of the synthesis and release of IL-10 during early infection, we investigated the autocrine effects of IL-6, IL-12 , tumor necrosis factor alpha (TNF-alpha), and IL-10 itself, as well as the exocrine effect of IFN-gamma on the production of macrophage derived IL-10 with lipoprotein as a stimulant ... Both autocrine ( IL-10 ) and exocrine ( IFN-gamma ) inhibition of IL-10 production resulted in an increase in the production of the proinflammatory cytokines IL-6 and IL-12
Glowacka et al., Scand J Immunol 2002 (Diabetes Mellitus, Type 1...) : The effect of LPS on neutrophils from patients with high risk of type 1 diabetes mellitus in relation to IL-8, IL-10 and IL-12 production and apoptosis in vitro
Nakanishi et al., Kekkaku 2002 (Hypersensitivity) : However, it was later shown that IL-12 and IL-18 without TcR engagement can induce IFN-gamma in Th1 cells and nonpolarized T cells
Egwuagu et al., J Immunol 2002 : We also demonstrate that IL-12 induced STAT4 activation is inhibited in Th2 cells that express high levels of SOCS3 whereas IL-4/STAT6 signaling is constitutively activated in Th2 cells, but not Th1 cells, with high SOCS1 expression
Wang et al., J Immunol 2002 (Dermatitis, Contact) : Because mature IL-18 requires cleavage of immature precursors by caspase-1, we further examined IL-12 induced IFN-gamma production in caspase-1 ( -/- ) LN cells
Lang et al., J Immunol 2002 (Infertility, Male...) : Transgenic macrophages displayed suppressed production of TNF-alpha and IL-12 upon stimulation with LPS
Fagiolo et al., Autoimmunity 2002 (Anemia, Hemolytic, Autoimmune) : Furthermore, it has been observed a significant increase in IL-12 production in LPS stimulated cultures from healthy controls, but not in AIHA patients, that shows IL-10 increased levels, which could cause a secondary decrease in IFN-gamma production and a stimulation of Th2 differentiation
Aramaki et al., Biol Pharm Bull 2002 : NADPH-oxidase may contribute to IL-12 production in macrophages stimulated with CpG phosphorothioate oligodeoxynucleotides ... IL-12 secretion was also inhibited by NADPH-oxidase inhibitors, suggesting that ROS generated by NADPH-oxidase is involved in the immunostimulatory effect of CpG-ODN
Puliti et al., Arthritis Rheum 2002 (Arthritis, Infectious...) : The amelioration of articular lesions is mostly attributable to IL-12 induced IFN gamma , but with a relevant participation of IL-12 induced IL-10 ... The amelioration of articular lesions is mostly attributable to IL-12 induced IFN gamma, but with a relevant participation of IL-12 induced IL-10
Matsuzaki et al., Eur J Immunol 2002 (Listeriosis) : All the results suggest that the Vgamma1+ gamma delta T cells participate in immune surveillance against intracellular bacterial infection through quick production of IFN-gamma in response to infection induced IL-12 without antigen-driven clonal expansion and maturation
Demangel et al., Eur J Immunol 2002 (Tuberculosis) : Here we examined whether the production of IL-10 by DC affected their IL-12 response to mycobacterial infection and the generation of protective immune responses in vivo
Corinti et al., J Leukoc Biol 2002 (HIV Infections) : Stimulation of DC with IFN-gamma increased the release of interleukin (IL)-12 and tumor necrosis factor-alpha and inhibited the production of IL-10 ... Stimulation of DC with IFN-gamma increased the release of interleukin (IL)-12 and tumor necrosis factor-alpha and inhibited the production of IL-10
Alleva et al., J Leukoc Biol 2002 : Thiazolidinediones and 15d-PGJ2 suppressed lipopolysaccharide (LPS) induced PEM production of NO and IL-12 ( p40 ) to a greater extent than IL-6 and TNF-alpha production
Wittmann et al., J Immunol 2002 (MAP Kinase Signaling System) : The suppression of IL-12 was observable on the mRNA and protein levels and was not due to endogenous production of known IL-12 antagonists ( i.e., IL-10, IL-4, and PGE ( 2 ) ), to an increased number of cells undergoing apoptosis, nor to down-regulation of the IFN-gamma or CD40 receptor ... The suppression of IL-12 was observable on the mRNA and protein levels and was not due to endogenous production of known IL-12 antagonists ( i.e., IL-10, IL-4, and PGE ( 2 ) ), to an increased number of cells undergoing apoptosis, nor to down-regulation of the IFN-gamma or CD40 receptor
Domeika et al., Vet Immunol Immunopathol 2002 : The IFN-gamma production induced by poIL-12 fusion protein, as well as heterodimeric poIL-12 and human IL-12 , was markedly dependent on the presence of human IL-18 ( huIL-18 )
Karni et al., J Neuroimmunol 2002 (Multiple Sclerosis) : In controls and relapsing remitting MS neutralizing anti-IL-12 and anti-IL-18 alone equally suppressed IFN-gamma production whereas in progressive MS, maximum suppression of IFN-gamma was only observed when neutralizing anti-IL-12 and anti-IL-18 were given together, suggesting that in progressive MS, IL-12 and IL-18 function in a non linked manner to induce IFN-gamma
Speirs et al., J Immunol 2002 (Leishmaniasis, Cutaneous) : NF-kappa B2 is required for optimal CD40 induced IL-12 production but dispensable for Th1 cell Differentiation
Kuribayashi et al., Clin Exp Allergy 2002 (Asthma...) : We investigated whether IL-12 administered after antigen challenge could diminish airway hyper-reactivity (AHR) and eosinophilia in mice actively sensitized to ovalbumin ... We also have investigated the ability of administered IL-12 to induce IL-18 receptor (IL-18R) expression that may lead possible synergic action of IL-12 with endogenous IL-18 ... Mice were analysed for effects of IL-12 on AHR , inflammatory cell infiltration and cytokine levels in lung tissue as well as serum immunoglobulin ( Ig ) E levels ... These data indicate that IL-12 can attenuate established antigen induced AHR and inflammation
Yagita et al., In Vivo 2002 (Adenocarcinoma...) : IL-X , components derived from the same Basidiomycetes, also enhanced the serum IL-12 level in H-2b mice in the early stage of tumor like AHCC, and maintained serum IL-12 at a level higher than the normal value accompanying tumor growth, whereas AHCC did not restore the lowered serum IL-12 level accompanying tumor growth
Langrish et al., Clin Exp Immunol 2002 : Cord DCs were also unable to produce IL-12p70 , failed to down-regulate expression of the chemokine receptor CCR5 and induced lower levels of IFN-gamma production from allogeneic naive CD4+ T cells than their adult counterparts
Wang et al., Zhonghua Gan Zang Bing Za Zhi 2002 (Hepatitis B, Chronic) : [ Effect of IL-12 on IFN-gamma and IL-10 produced by peripheral blood mononuclear cells in patients with chronic hepatitis B virus infection during IFN-alpha treatment ] ... [ Effect of IL-12 on IFN-gamma and IL-10 produced by peripheral blood mononuclear cells in patients with chronic hepatitis B virus infection during IFN-alpha treatment ] ... To investigate the effect of IL-12 on IFN-gamma and IL-10 production of peripheral blood mononuclear cells ( PBMC ) in chronic hepatitis B virus infection patients during IFN-alpha treatment ... To investigate the effect of IL-12 on IFN-gamma and IL-10 production of peripheral blood mononuclear cells ( PBMC ) in chronic hepatitis B virus infection patients during IFN-alpha treatment ... In the responders, the enhancing effect of IL-12 on IFN-gamma production was significantly greater after IFN-alpha treatment than before the treatment ... The production of IL-10 was suppressed in the presence of IL-12 after 3 months and 6 months of IFN-alpha treatment ... In the same treatment time, the level of IFN-gamma in the presence of IL-12 was significantly higher than that in the absence of IL-12 ... To the nonresponders, the enhancing effect of IL-12 on IFN-gamma production was also significantly increased after IFN-alpha treatment ... Moreover, in the same treatment time, the level of IFN-gamma in the presence of IL-12 was significantly higher than that in the absence of IL-12 ... The enhancing effect of IL-12 on IFN-gamma production of PBMC in patients with chronic hepatitis B virus infection is increased during IFN-alpha treatment
Yamakami et al., Immunology 2002 (Leishmaniasis, Cutaneous) : Furthermore, production of IL-12 from the spleen cells stimulated by L. major was suppressed in the presence of IL-4 and IL-10 ... Furthermore, production of IL-12 from the spleen cells stimulated by L. major was suppressed in the presence of IL-4 and IL-10
Derre et al., J Immunol 2002 (Melanoma) : Expression of CD94/NKG2-A on human T lymphocytes is induced by IL-12 : implications for adoptive immunotherapy ... Expression of CD94/NKG2-A on human T lymphocytes is induced by IL-12 : implications for adoptive immunotherapy ... We show that IL-12 induces the expression of NKG2-A and/or CD94 by CD8 ( + ) T cells in culture, and that this induction was mediated neither by IFN-gamma nor by IL-15 ... We show that IL-12 induces the expression of NKG2-A and/or CD94 by CD8 ( + ) T cells in culture, and that this induction was mediated neither by IFN-gamma nor by IL-15 ... We show that IL-12 induces the expression of NKG2-A and/or CD94 by CD8 ( + ) T cells in culture, and that this induction was mediated neither by IFN-gamma nor by IL-15 ... We show that IL-12 induces the expression of NKG2-A and/or CD94 by CD8 ( + ) T cells in culture, and that this induction was mediated neither by IFN-gamma nor by IL-15 ... We also show, using the redirected killing assay, that IL-12 induced expression of both CD94 and NKG2-A led to the acquisition by T cells of a functional inhibitory receptor ... We also show, using the redirected killing assay, that IL-12 induced expression of both CD94 and NKG2-A led to the acquisition by T cells of a functional inhibitory receptor
Bao et al., J Immunol 2002 (Diabetes Mellitus, Type 1...) : Treatment of CD4 ( + ) T cells with estrogen significantly increased, whereas testosterone treatment decreased the IL-12 induced production of IFN-gamma ... We then examined whether the change in IL-12 induced IFN-gamma production by treatment with sex hormones was due to the regulation of STAT4 activation ... We conclude that the increased susceptibility of female NOD mice to the development of autoimmune diabetes could be due to the enhancement of the Th1 immune response through the increase of IL-12 induced STAT4 activation by estrogen
Coulombe et al., J Leukoc Biol 2002 : It is interesting that ET-1 and ET-3 inhibited ( 25-30 % ) mRNA expression of Th2-type cytokines ( IL-4, IL-10, and IL-13 ) and increased IL-12 release ( 39 % and 41 %, respectively ) without affecting MIP-1alpha and NO production
Boneberg et al., Inflamm Res 2002 : G-CSF influences monocyte functions in an anti-inflammatory way : The stimulation of monocytes with G-CSF results in an attenuation of LPS induced release of IL-1beta, TNF-alpha, IL-12 and IL-18
Aliberti et al., Mol Immunol 2002 : Our findings indicate that the potent IL-12 response observed is highly dependent on both the chemokine receptor CCR5 and G ( i ) -protein coupled signaling
Liebau et al., Anticancer Res 2002 (Bone Neoplasms...) : The presented study demonstrates that IL-12 and IL-18, or even more a combined application of both cytokines, induce IDO expression besides the known pathway via IFN-gamma
Belladonna et al., J Immunol 2002 : In addition, the in vitro effects of IL-23-Ig did not appear to require IL-12Rbeta2 or to be mediated by the production of IL-12
Kuroda et al., J Immunol 2002 : However, IFN-gamma production in response to IL-12 or IL-12 plus IL-18 was much lower in macrophages from BALB/c mice than other strains
Schmidt et al., J Immunol 2002 : IL-12 supports responses when IL-12Rbeta1-deficient mice are used as recipients for the adoptively transferred CD8 T cells, demonstrating that the IL-12 is acting directly on the T cells rather than on host APC
Kang et al., Biochem Pharmacol 2002 : Berberine induced IL-12 p40 production and activation of p38 mitogen activated protein kinase ( MAPK ) in dose dependent manners, which were significantly inhibited by p38 MAPK inhibitors and yohimbine, indicating that p38 MAPK and alpha ( 2 ) -adrenergic receptor were involved in the induction of IL-12 p40 production in mouse macrophages by berberine ... Berberine induced IL-12 p40 production and activation of p38 mitogen activated protein kinase ( MAPK ) in dose dependent manners, which were significantly inhibited by p38 MAPK inhibitors and yohimbine, indicating that p38 MAPK and alpha ( 2 ) -adrenergic receptor were involved in the induction of IL-12 p40 production in mouse macrophages by berberine
Companjen et al., Int Immunol 2002 : IL-6, IL-8, tumor necrosis factor (TNF)-alpha, IL-12 and IL-1 beta were induced upon CD40 ligation and IFN-gamma stimulation, while IL-10 could be induced by CD40 ligation alone and was reduced again by the addition of IFN-gamma ... IL-6, IL-8, tumor necrosis factor (TNF)-alpha, IL-12 and IL-1 beta were induced upon CD40 ligation and IFN-gamma stimulation, while IL-10 could be induced by CD40 ligation alone and was reduced again by the addition of IFN-gamma
Guo et al., Gene Ther 2002 (Lung Neoplasms...) : Furthermore, a significant increase of IL-4 production within the tumors was observed early after the AdMDC administration and was followed by the increase of IL-12 and IL-2 production
Scanga et al., J Immunol 2002 (Toxoplasmosis, Animal) : Defective IL-12 responses to T. gondii Ags ( soluble tachyzoite Ag ( STAg ) ) were observed in MyD88 ( -/- ) peritoneal macrophages, neutrophils, and splenic dendritic cells ( DC )
Hodge et al., J Immunol 2002 : We subsequently expanded our search for other chemokine receptor genes absent from the microarray and found an IL-2- and IL-12 dependent decrease in CXCR3 receptor mRNA expression in NK92 cells
Jump et al., J Immunol 2002 : Exogenous IL-12 enhances both IL-10 and IFN-gamma secretion by activated PP MC. Cell surface marker analysis reveals that PP T cells consist of activated and memory subpopulations compared with the predominantly naive T cells identified in the PLN and mesenteric lymph nodes ... Exogenous IL-12 enhances both IL-10 and IFN-gamma secretion by activated PP MC. Cell surface marker analysis reveals that PP T cells consist of activated and memory subpopulations compared with the predominantly naive T cells identified in the PLN and mesenteric lymph nodes
Smeltz et al., J Immunol 2002 : In the absence of IL-4, IL-12 dependent up-regulation of IL-18Ralpha/IL-12Rbeta2 was independent of IFN-gamma ... In the absence of IL-4, IL-12 dependent up-regulation of IL-18Ralpha/IL-12Rbeta2 was independent of IFN-gamma
Natarajan et al., J Immunol 2002 (Demyelinating Diseases...) : In vitro treatment of activated T cells with curcumin inhibited IL-12 induced tyrosine phosphorylation of Janus kinase 2, tyrosine kinase 2 , and STAT3 and STAT4 transcription factors ... In vitro treatment of activated T cells with curcumin inhibited IL-12 induced tyrosine phosphorylation of Janus kinase 2 , tyrosine kinase 2, and STAT3 and STAT4 transcription factors
Lammas et al., Int J Exp Pathol 2002 (Genetic Predisposition to Disease...) : These experiments of nature demonstrate that IL-12 dependent IFNgamma production is critical for granuloma formation and therefore host immunity against such pathogens
Mazzeo et al., Cytokine 2002 : We thus studied the effect of reduced glutathione ( GSH ) and N-acetyl-cysteine (NAC) on IL-12 p75 production by human THP-1 cell stimulated with IFN-gamma and Staphylococcus aureus Cowan strain I ( SAC ), using ELISAs specific for IL-12 p75 or the p40 subunit
Yang et al., Neuroendocrinology 2002 : LPS induced IL-12 p40 gene induction in the pituitary gland was suppressed after beta-adrenoceptor agonist pretreatment in vivo ... LPS induced IL-12 p40 gene induction in the pituitary gland was suppressed after beta-adrenoceptor agonist pretreatment in vivo ... Both IL-12 p40 gene induction and IL-12 production were also observed when freshly isolated pituitary glands from non treated SD rats were incubated with LPS in vitro
Lee et al., Clin Exp Immunol 2002 (Tuberculosis, Multidrug-Resistant) : Three out of 18 patients with MDRTB of greater than 4 years duration showed significantly elevated IL-12 p70 production, induced by in vitro PPD stimulation of their PBMC, when compared with data from HTR
Baldwin et al., Vet Immunol Immunopathol 2002 : In contrast, stimulation by Con A or PMA/ionomycin induced efficient replication but only low level IFN-gamma production which was not enhanced by the presence of IL-12
Gergely et al., J Immunol 2002 (Lupus Erythematosus, Systemic) : Ab-mediated IL-10 blockade or stimulation with antagonistic lymphokine IL-12 normalized baseline and CD3/CD28 induced changes in ROI production and pH(i) with no impact on Delta psi ( m ) of lupus PBL
Ohata et al., Clin Exp Immunol 2002 (Graft vs Host Disease...) : Although T cell mediated effector function at 14 days post-BMT assessed by IFNgamma expression and cytotoxicity against host alloantigen was comparable between both co-stimulatory blockades, IL-12 mRNA expression was preferentially reduced by CD40 blockade
Obermeier et al., Eur J Immunol 2002 (Acute Disease...) : Colonic expression of IFN-gamma and TNF was elevated ( 200- and 150-fold, respectively ) and IFN-gamma and IL-12 secretion from lymph node cells was increased 5,000- and 8-fold, respectively, compared to GpG-ODN treated controls ... Colonic expression of IFN-gamma and TNF was elevated ( 200- and 150-fold, respectively ) and IFN-gamma and IL-12 secretion from lymph node cells was increased 5,000- and 8-fold, respectively, compared to GpG-ODN treated controls
Nakajima et al., Eur J Immunol 2002 : The induction of another Th1 chemokine receptor CCR5 absolutely required IL-12 stimulation and STAT4 involvement
McDowell et al., Infect Immun 2002 : Here we show that in contrast to L. major, Leishmania species responsible for visceral disease ( L. donovani ), as well as species associated with persistent, cutaneous lesions and occasional systemic disease ( L. tropica ), did not induce CD40L dependent IL-12p70 production, despite comparable levels of uptake by DCs ... Up-regulated surface expression of CD40 did not correlate with IL-12p70 production, and appreciable CD40L induced IL-12p40 secretion was observed in uninfected as well as infected DCs, regardless of species ... The intrinsic differences in the ability of Leishmania species to prime DCs for CD40L dependent IL-12p70 secretion may account, at least in part, for the evolution of healing and nonhealing forms of leishmanial disease
Pascual et al., Infect Immun 2002 : The absence of IL-12 was not due to overt increases in production of either IL-12 p40 or IL-10 ... The absence of IL-12 was not due to overt increases in production of either IL-12 p40 or IL-10
Xu et al., Am J Transplant 2002 : Porcine xenoantigen induced IFN-gamma production was only partially dependent on IL-12
Pflanz et al., Immunity 2002 : IL-12 triggers Th1 polarization of naive CD4 ( + ) T cells and secretion of IFN-gamma
Chen et al., Zhonghua Jie He He Hu Xi Za Zhi 2002 (Tuberculosis) : To investigate the effect of IL-12 on the production of IFN-gamma and IL-4 by peripheral blood mononuclear cells ( PBMC ) from patients with tuberculosis ... To investigate the effect of IL-12 on the production of IFN-gamma and IL-4 by peripheral blood mononuclear cells ( PBMC ) from patients with tuberculosis ... Compared with the PPD group, IL-12 increased the production of IFN-gamma ( 321.6 +/- 87.7 vs 452.5 +/- 111.4, 387.0 +/- 70.8 vs 515.4 +/- 44.1, respectively ), but decreased the production of IL-4 ( 54.6 +/- 11.0 vs 41.3 +/- 13.5, 55.1 +/- 9.5 vs 38.2 +/- 12.9, respectively ) in the patients and the healthy individuals ; anti-IL-12 blocked this effect ... IL-12 induced IFN-gamma production and inhabited IL-4 expression, modulating the balance between TH ( 1 ) and TH ( 2 ) cytokines, suggesting that IL-12 enhances the host defense against tuberculous infection
Hope et al., J Immunol Methods 2002 : Some of these mAb neutralised the ability of rbo IL-12 to induce IFNgamma synthesis by bovine cells
Kim et al., J Immunol 2002 (Acne Vulgaris) : P. acnes also induced activation of IL-12 p40 promoter activity via TLR2
Ausiello et al., J Infect Dis 2002 : Although they did not affect interleukin (IL)-10 production by lipopolysaccharide (LPS) stimulated DCs, both nPT and dPT strongly synergized with LPS for IL-12 production
Bouma et al., Gastroenterology 2002 (Colitis...) : We observed a remarkably higher serum IL-12 response to LPS in susceptible SJL/J mice ... These data strongly suggest that the tendency to mount a high LPS induced IL-12 response and susceptibility to TNBS induced colitis are related and that in fact a genetically determined high IL-12 response is involved in the immunologic basis of susceptibility to colitis
Lapteva et al., Biochem Biophys Res Commun 2002 : In DCs, lipopolysaccharide (LPS) induced production of tumor necrosis factor-alpha (TNF-alpha), interleukin-(IL)-1alpha, IL-10, IL-12 , and IL-18 was significantly inhibited by captopril
Kikkawa et al., Int Immunol 2002 : Decreased IL-12 induced STAT4 phosphorylation was also observed
Werling et al., J Leukoc Biol 2002 : These observations suggest that while IL-10 , produced by MoDC as a result of exposure to live BRSV, may affect IL-12 and IL-15 synthesis by MoDC, it does not appear to affect the cytokine response of BRSV-specific memory CD4 ( + ) T cells
Fukao et al., Nat Immunol 2002 : PI3K mediated negative feedback regulation of IL-12 production in DCs ... We show here that phosphoinositide 3-kinase (PI3K) negatively regulates IL-12 synthesis by DCs
Freudenberg et al., J Immunol 2002 : This pathway is based on a synergism of IFN-alphabeta and IL-18, and is independent of IL-12 signaling yet dependent on STAT4
Uchi et al., J Allergy Clin Immunol 2002 (MAP Kinase Signaling System) : PTL inhibited the upregulation of CD80, CD83, CD86, CD40, and MHC class II ; the allostimulatory function ; the production of TNF-alpha and IL-12 ; and the downregulation of FITC labeled dextran uptake in human monocyte derived DCs stimulated with LPS but not with TNF-alpha
Toyokawa et al., Anticancer Res 2002 : Both OK432 and KP-40 were found to up-regulate the activity of myeloid-lineage DCs : the expression of major histocompatibility complex ( MHC) class II molecules and adhesion/costimulatory molecules increased , and the production of IL-12 also increased
Butcher et al., Infect Immun 2002 : Macrophages ( Mphi ) infected with tachyzoites of the opportunistic protozoan Toxoplasma gondii are blocked in production of the proinflammatory cytokines tumor necrosis factor alpha (TNF-alpha) and interleukin-12 (IL-12) in response to lipopolysaccharide (LPS) triggering, and this is associated with parasite induced inhibition of NFkappaB translocation
Vicari et al., J Exp Med 2002 : In particular, CpG plus anti-IL-10R enhanced the TAA-specific immune response and triggered de novo IL-12 production
McDyer et al., J Immunol 2002 : These data demonstrate that IL-2 plays a critical role in the regulation of Th1 and Th2 responses and impacts both IL-12 dependent and -independent IFN-gamma production
Dabrowska-Iwanicka et al., J Cancer Res Clin Oncol 2002 (Colonic Neoplasms...) : We showed that antitumour activity of this combined therapy is mediated by multiple mechanisms : not only is enhancement of the antiangiogenic activity mediated by TNP-470 and IL-12 but also by the synergistic cytostatic/cytotoxic action of IL-12 induced IFN-gamma and TNP-470 or cisplatin on tumour cells
Saudemont et al., Leukemia 2002 (Acute Disease...) : CD154 triggers CD40 on antigen presenting cells, thus inducing antigen presentation to the immune system and production of IL12
McBride et al., Cell Immunol 2002 : IL-10 inhibited LPS induced upregulation of costimulatory molecules, MHC Class II, and the secretion of IL-12 , TNF-alpha, IL-6, and IL-1beta by DCs, although it upregulated the SLAM ( CD150 ) expression at both the mRNA and protein levels ... IL-10 inhibited LPS induced upregulation of costimulatory molecules, MHC Class II, and the secretion of IL-12 , TNF-alpha, IL-6, and IL-1beta by DCs, although it upregulated the SLAM ( CD150 ) expression at both the mRNA and protein levels
Wilkin et al., Eur J Immunol 2002 : We observed that ATPgammaS potentiated the IL-12p40 release induced by TNF-alpha , but also by lipopolysaccharides (LPS) and soluble CD40 ligand ( sCD40L ) ... We observed that ATPgammaS potentiated the IL-12p40 release induced by TNF-alpha, but also by lipopolysaccharides (LPS) and soluble CD40 ligand ( sCD40L ) ... We observed that ATPgammaS potentiated the IL-12p40 release induced by TNF-alpha, but also by lipopolysaccharides (LPS) and soluble CD40 ligand ( sCD40L )
Shurin et al., Int J Cancer 2002 : Interestingly, CD40 ligation on DCs generated from tumor-bearers did not result in inducible expression of IL-12 protein or IL-12 p40 mRNA ... However, Staphylococcus aureus induced IL-12 production by DCs was not altered in tumor-bearers, confirming that inhibition of IL-12 production by DCs generated in vitro from tumor bearing mice was due to reduced expression of CD40 on DCs ... In addition, endogenous IL-10 produced by colon carcinoma cells inhibited CD40 dependent IL-12 production by DCs since tumor induced inhibition of IL-12 production was abrogated by neutralizing anti-IL-10 antibody ... In addition, endogenous IL-10 produced by colon carcinoma cells inhibited CD40 dependent IL-12 production by DCs since tumor induced inhibition of IL-12 production was abrogated by neutralizing anti-IL-10 antibody
Morinobu et al., Proc Natl Acad Sci U S A 2002 : STAT4 serine phosphorylation is critical for IL-12 induced IFN-gamma production but not for cell proliferation
Finnegan et al., J Immunol 2002 (Arthritis, Experimental) : To determine whether the capacity of IL-4 to inhibit disease is dependent on IL-4 mediated regulation of IL-12 , we assessed the requirement for IL-4 in modulating development of PGIA ... Taken together, these results suggest that IL-4 regulates the severity of disease in PGIA by controlling IL-12 production, which in turn regulates the magnitude of IFN-gamma expression through a Stat4 dependent pathway
Möttönen et al., Arthritis Res 2002 (Arthritis, Rheumatoid) : We have studied the CD154 induced IL-12p40 and IL-12p70 production by synovial fluid ( SF ) macrophages from patients with RA ... CD40 ligation induced the secretion of IL-12p40 but not IL-12p70
Yun et al., Infect Immun 2002 : P. gingivalis LPS was shown to enhance IL-12 induction of IFN-gamma in T cells in a manner independent from TNF-alpha contribution ... The levels of T-cell IL-12 receptors were not affected by P. gingivalis LPS and played only a minor role in the magnitude of the IFN-gamma response ... We previously reported that the major cysteine proteinases ( gingipains ) copurifying with LPS in this organism were responsible for reduced IFN-gamma accumulation in the presence of IL-12
Takakura et al., Inflamm Res 2002 (Disease Models, Animal...) : In the presence of LPS or anti-CD40 mAb, TNF-alpha and IL-12p70 release from macrophages of mutant mice was significantly higher than that from macrophages of wild-type mice
Grzela et al., Int J Mol Med 2002 : Influence of allergen-specific immunotherapy on IL-4 dependent IL-12 production by monocytes ... Since IFN-gamma production is stimulated by IL-12 , which in turn is down-regulated by IL-4, the aim of study was to analyze the influence of AIT on the network of these cytokines
Yoo et al., J Immunol 2002 : IL-12 induces T cell proliferation and IFN-gamma secretion from activated T cells ... In this study, we demonstrated that IL-12 activates phosphatidylinositol 3-kinase (PI3K)/Akt pathway in murine CD4 ( + ) T cells, and that this signaling pathway is required for IL-12 induced T cell proliferation and antiapoptotic function, but not for IFN-gamma induction ... These results suggest that PI3K/Akt pathway is critical for mediating IL-12 induced CD4 ( + ) T cell responses such as T cell proliferation and survival
Butler et al., J Immunol 2002 (Alveolitis, Extrinsic Allergic...) : We also showed that this was explained in part by differential regulation of IL-12 by IL-4
Seki et al., J Immunol 2002 (Inflammation...) : As TLR2 KO, but not TLR4 KO, mice showed partial impairment in their capacity to produce IL-12, IFN-gamma, and TNF-alpha, TLR2 activation partly contributed to the induction of IL-12 mediated IFN-gamma production ... These results indicated a critical role for TLRs/MyD88 dependent IL-12/TNF-alpha production and for IL-12- and IL-18 mediated IFN-gamma production in early phase clearance of LM
Leifeld et al., Hepatology 2002 (Acute Disease...) : In murine models, overexpression of interleukin (IL)-12 and interferon (IFN)-gamma can induce severe liver damage, whereas IL-10 has anti-inflammatory and hepatoprotective properties
Inui et al., Int Immunopharmacol 2002 : Similarly, endomorphin-1 suppressed IL-10 and IL-12 productions in response to LPS
Murata et al., Eur J Immunol 2002 (Anoxia) : Murine mature splenic DC with elevated intracellular glutathione, pretreated with IL-18, strikingly augmented the production of IFN-gamma in response to IL-12 , whereas intracellular glutathione deprivation ablated this effect of IL-18
Li et al., Gastroenterology 2002 (Fatty Liver) : Leptin deficiency increases hepatic IL-12 and reduces hepatic IL-15 expression
Itoh et al., Transplantation 2002 : In parallel to ICAM-1 up-regulation, IL-18 significantly enhanced the production of IFN-gamma and IL-12 in MLR
Takada et al., Tissue Antigens 2002 (Genetic Predisposition to Disease...) : Interleukin-18 (IL-18) and interleukin-12 (IL-12) synergistically stimulate interferon-gamma (IFN-gamma) production from Th1 cells
Panther et al., J Immunol 2002 (Calcium Signaling) : However, LPA inhibited in a pertussis toxin-insensitive manner the secretion of IL-12 and TNFalpha as well as enhanced secretion of IL-10 from mature DCs
Rizzitelli et al., J Immunol 2002 (Lymphopenia) : Although IL-4 restores IL-12 production by CD3epsilon ( -/- ) SDCs, anti-IL-4 Abs inhibited only partially the IL-12 production in coculture of CD3epsilon ( -/- ) DCs and WT T cells
Weijzen et al., J Immunol 2002 : Furthermore, coculture of DCs with cells expressing Notch ligand Jagged-1 induced up-regulation of maturation markers, IL-12 production, T cell proliferative responses, and IFN-gamma production ... Furthermore, coculture of DCs with cells expressing Notch ligand Jagged-1 induced up-regulation of maturation markers, IL-12 production, T cell proliferative responses, and IFN-gamma production
Nguyen et al., J Immunol 2002 (Herpesviridae Infections) : IL-12/STAT4 was critical for NK cell IFN-gamma expression, whereas IFN-alphabeta/STAT1 were required for induction of cytotoxicity
Nishikomori et al., J Immunol 2002 : We found that such cells were still unable to exhibit IL-12 mediated IFN-gamma production ... We then showed that constructs that were unable to support STAT4 tyrosine phosphorylation ( in D10 cells ) as a result of mutation were also incapable of supporting IL-12 induced IFN-gamma production ( in IL-12Rbeta2 ( -/- ) cells )
Ghaemmaghami et al., Clin Exp Allergy 2002 (Hypersensitivity, Immediate) : The suppression of IL-12 production was due to the cleavage of CD40 by the proteolytic activity of Der p 1, hence rendering the DCs less responsive to stimulation through the CD40L-CD40 pathway
Yamamoto et al., Biochem Biophys Res Commun 2002 : IL-12 activates STAT4 by inducing tyrosine phosphorylation, homo-dimerization, and nuclear translocation in NK cells and thereby stimulates proliferation and activation of these cells
Pan et al., Mol Ther 2002 (Colonic Neoplasms...) : The results demonstrated that OX40 ligation of CD4 ( + ) T cells facilitated the development of primary and memory CTL responses against tumorcells and that coordinated immune activation by IL-12 , anti-4-1BB, and anti-OX40 resulted in a significantly higher survival rate in mice with large tumor burdens
Drechsler et al., J Leukoc Biol 2002 : Inhibition of IL-12 by FcgammaR cross linking was not mediated by TNF-alpha , as the presence of an anti-TNF-alpha Ab could not restore the reduced IL-12 production
Losana et al., J Leukoc Biol 2002 (HIV Infections) : IFN-gamma and IL-12 differentially regulate CC-chemokine secretion and CCR5 expression in human T lymphocytes
Kim et al., Blood 2002 : The 4-1BB was induced preferentially on the CB CD28 ( - ) CD8 ( + ) T cells when CD28 down-regulation was induced by interleukin 15 (IL-15) and IL-12 stimulation
Dredge et al., Cancer Immunol Immunother 2002 (Neoplasms) : Th1-type responses which comprise cell mediated immunity are characterised by the secretion of interferon-gamma by T cells, which is induced by antigen presenting cell (APC) derived IL-12
Aksoy et al., Eur J Immunol 2002 : Critical role of protein kinase C epsilon for lipopolysaccharide induced IL-12 synthesis in monocyte derived dendritic cells ... Inhibition of PKC activation using bisindolylmaleimide ( Bis ), a pan-PKC inhibitor, was associated with a dose dependent decrease of LPS induced IL-12 production ... Inhibition of LPS induced PKC activity using pseudosubstrate peptides specific for PKC isoforms established that PKC epsilon but not PKC alpha/beta was involved in the production of IL-12 and TNF-alpha ... Inhibition of LPS induced PKC activity using pseudosubstrate peptides specific for PKC isoforms established that PKC epsilon but not PKC alpha/beta was involved in the production of IL-12 and TNF-alpha
Bhandari et al., Food Chem Toxicol 2002 (Drug-Induced Liver Injury...) : Selective increase in proinflammatory T helper ( Th ) 1-cytokines ( IL-12 and IFNgamma ) and TNFalpha with no alteration in Th2-cytokines ( IL-4, IL-6 and IL-10 ) suggest the involvement of IL-12 , produced by Kupffer cells, in induction of IFNgamma production by natural killer ( NK ) cells and/or NK1+ T cells, which can undergo a positive amplification loop with TNFalpha produced by macrophages or other hepatic cells to elicit the toxic reaction
Symons et al., J Gen Virol 2002 (Disease Models, Animal...) : A bioassay that measured the interleukin (IL)-12 induced production of interferon (IFN)-gamma from mouse splenocytes was used to identify a soluble factor in the supernatants of vaccinia virus ( VV ) -infected cells that inhibited the production of IFN-gamma ... Thus the inhibition of IL-12 induced IFN-gamma production was due to indirect effects of C12L on IL-18, illustrating the synergistic action of these pro-inflammatory cytokines
Barone et al., Clin Exp Immunol 2002 : These results suggest that blocking B7-CTLA-4 interactions in the presence of IL-12 prevents the induction of low-dose oral tolerance at the Th1 cell level
Kitaura et al., J Immunol 2002 : Apoptosis of the adherent cells was markedly inhibited by anti-Fas ligand (FasL) Ab. RT-PCR and FACS analyses revealed that TNF-alpha up-regulated Fas transcription to lead to Fas expression on the surfaces of the adherent cells, whereas IL-12 could not induce Fas on the cells ... In contrast, IL-12 induced FasL transcription to lead to FasL expression on the surfaces of nonadherent bone marrow cells, whereas TNF-alpha could not induce FasL on the cells ... In contrast, IL-12 induced FasL transcription to lead to FasL expression on the surfaces of nonadherent bone marrow cells, whereas TNF-alpha could not induce FasL on the cells ... These results implied that apoptosis of the adherent cells in bone marrow cells might be caused by interaction between TNF-alpha induced Fas on the adherent cells and IL-12 induced FasL on the nonadherent cells
Omata et al., J Immunol 2002 : The production of IL-10 , a potent endogenous IL-12 inhibitor , was not affected by MCP-1
Kimura et al., J Immunol 2002 (Inflammation) : The antiviral effect depended on the production of IL-12 and TNF-alpha by activated APCs ; however, it was mediated primarily by IFN-gamma produced by NK cells, and possibly T cells, that were activated by IL-12
Khaled et al., Biotechniques 2002 : IL-12 and IL-4 also promote memory CD8+ survivaL BAFF is required for survival of mature B cells
Marth et al., Gastroenterology 2002 (Hypersensitivity, Delayed...) : The decreased monocyte IL-12 levels may result in reduced peripheral and mucosal IFN-gamma production and lead to an increased susceptibility to T. whipplei infection in certain hosts
Fujimoto et al., Int Immunol 2002 : Since IL-12 induced STAT4 and IL-4 induced STAT6 activation is sustained in SOCS-1 ( -/- ) T cells, the enhanced T ( h ) functions in SOCS-1 ( -/- ) and SOCS-1 ( +/- ) mice appear to be due to the enhanced effects of these cytokines ... Since IL-12 induced STAT4 and IL-4 induced STAT6 activation is sustained in SOCS-1 ( -/- ) T cells, the enhanced T ( h ) functions in SOCS-1 ( -/- ) and SOCS-1 ( +/- ) mice appear to be due to the enhanced effects of these cytokines
Plesner et al., Scand J Immunol 2002 (Diabetes Mellitus, Type 1) : In this study, peripheral blood monocytes were differentiated to macrophages with macrophage-colony stimulating factor ( M-CSF ) to determine lipopolysaccharide (LPS) stimulated tumour necrosis factor (TNF)-alpha, interleukin (IL)-1beta, IL-6, IL-12 and prostaglandin E-2 ( PGE-2 ) secretion from hetero- or homozygous HLA DQB1*0201 and *0302 type 1 diabetes mellitus patients, first-degree relatives and homozygous HLA DQB1*0602 healthy controls
Masumi et al., FEBS Lett 2002 : IRF-8/ICSBP and IRF-1 cooperatively stimulate mouse IL-12 promoter activity in macrophages ... IRF-8/ICSBP and IRF-1 cooperatively stimulate mouse IL-12 promoter activity in macrophages ... We have previously shown that IL-12p40 expression is impaired in ICSBP-deficient mice and that transfection of ICSBP together with IRF-1 can activate IL-12p40 expression in mouse macrophage cells ... We have previously shown that IL-12p40 expression is impaired in ICSBP-deficient mice and that transfection of ICSBP together with IRF-1 can activate IL-12p40 expression in mouse macrophage cells ... We have previously shown that IL-12p40 expression is impaired in ICSBP-deficient mice and that transfection of ICSBP together with IRF-1 can activate IL-12p40 expression in mouse macrophage cells ... Together, these results indicate that ICSBP and IRF-1 cooperatively stimulate murine IL-12 transcription through a novel regulatory element in the murine promoter ... Together, these results indicate that ICSBP and IRF-1 cooperatively stimulate murine IL-12 transcription through a novel regulatory element in the murine promoter
Aggarwal et al., J Biol Chem 2003 : In contrast to IL-23, IL-12 had only marginal effects on IL-17 production
Cao et al., J Immunol 2002 (Fibrosarcoma) : Differential regulation of IL-12 and IL-10 gene expression in macrophages by the basic leucine zipper transcription factor c-Maf fibrosarcoma ... Regulation of proinflammatory IL-12 gene expression in phagocytes by the anti-inflammatory cytokine IL-10 represents a major homeostatic process underlying host-pathogen and host-self interactions ... However, IL-10 mediated inhibition of IL-12 production remains intact, indicating the existence of alternative mediators in the absence of c-Maf, consistent with the observation that a functional AP-1 is required for this pathway
Pien et al., J Immunol 2002 (Lymphocytic Choriomeningitis) : Production of IFN-gamma was MHC class I/CD8 dependent, but did not require IL-12 , NK cells, TCR-gammadelta T cells, MHC class II, or CD4 T cells
Al-Mohanna et al., J Leukoc Biol 2002 : IL-12 dependent nuclear factor-kappaB activation leads to de novo synthesis and release of IL-8 and TNF-alpha in human neutrophils ... IL-12 dependent nuclear factor-kappaB activation leads to de novo synthesis and release of IL-8 and TNF-alpha in human neutrophils ... In addition IL-12 induces the de novo synthesis and production of IL-8 and tumor necrosis factor alpha (TNF-alpha) in a calcium- and ROM dependent manner ... In addition IL-12 induces the de novo synthesis and production of IL-8 and tumor necrosis factor alpha (TNF-alpha) in a calcium- and ROM dependent manner
Bradford et al., J Biomed Sci 2002 : The regulation of macrophage IL-12 p40 secretion in response to stimulation with LPS and interferon-gamma (IFN-gamma) is dependent on the action of a nuclear transacting factor, interferon consensus sequence binding protein ( ICSBP ), a STAT1 dependent gene product ... The regulation of macrophage IL-12 p40 secretion in response to stimulation with LPS and interferon-gamma (IFN-gamma) is dependent on the action of a nuclear transacting factor, interferon consensus sequence binding protein ( ICSBP ), a STAT1 dependent gene product
Campbell et al., J Parasitol 2002 (Cryptosporidiosis...) : IL-12 is an important inducer of IFN-gamma , which probably accounted for susceptibility to infection
Dalpke et al., Immunobiology 2002 : In contrast, LTA suppressed IL12p40 secretion induced by CpG-DNA in RAW cells and peritoneal macrophages but not in dendritic cells
Nguyen et al., J Exp Med 2002 : In this study, cross linking B7-DC with the monoclonal antibody sHIgM12 directly potentiates dendritic cell ( DC ) function by enhancing DC presentation of major histocompatibility complex-peptide complexes, promoting DC survival ; and increasing secretion of interleukin (IL)-12p70 , a key T helper cell type 1 promoting cytokine
Kang et al., Cytokine 2002 (Leprosy, Lepromatous) : Upon stimulation with MLL , monocytes produced TNF-alpha and Interleukin-12 (IL-12) , which play a role in the innate immune response to infection ... Anti-TLR2 mAb blocked greater than 50 % of the MLL induced production of IL-12
van Seventer et al., J Neuroimmunol 2002 (Multiple Sclerosis) : Interferon-beta differentially regulates expression of the IL-12 family members p35, p40, p19 and EBI3 in activated human dendritic cells
Abraham et al., Int Immunol 2002 : IL-18 and IL-12 separately and synergistically enhanced MMP-2 , while TNF-alpha led to the elevation of MMP-9
Chesler et al., J Neuroimmunol 2002 (Encephalitis, Viral...) : In vitro, IL-12 induces the phosphorylation and nuclear localization of STAT4 in neuroblastoma cell lines
Palmer et al., Tissue Eng 2002 : We found that SIS partially suppressed Th1 cell expansion and secretion of interleukin 12 (IL-12) and interferon gamma (IFN-gamma) in a TGF-beta dependent manner, but Th1 cell expansion and IFN-gamma secretion could be fully overcome by addition of recombinant IL-12
Valenzuela et al., J Immunol 2002 : In addition, high CD25 expression is substantially prolonged in the presence of IL-12
Mold et al., J Immunol 2002 : In vitro, in the presence of LPS, CRP enhanced IL-10 synthesis and inhibited IL-12 synthesis by bone marrow macrophages from normal, but not gamma-chain-deficient mice ... In vitro, in the presence of LPS, CRP enhanced IL-10 synthesis and inhibited IL-12 synthesis by bone marrow macrophages from normal, but not gamma-chain-deficient mice ... In vitro, in the presence of LPS , CRP enhanced IL-10 synthesis and inhibited IL-12 synthesis by bone marrow macrophages from normal, but not gamma-chain-deficient mice
Tsai et al., Immunol Invest 2002 (Autoimmune Diseases...) : IFN-gamma enhanced the IL-12 production by normal PMN stimulated with LPS, but it inhibited the IL-12 production in PMN from active lupus patients in the presence of LPS ... IFN-gamma enhanced the IL-12 production by normal PMN stimulated with LPS, but it inhibited the IL-12 production in PMN from active lupus patients in the presence of LPS
Häusler et al., Eur J Neurosci 2002 (Encephalitis...) : While amounts of TNFalpha and IL-6 remained nearly unchanged, IFNgamma enhanced the production of IL-12 , MCP-1 and RANTES, but attenuated that of KC, MIP-1alpha and MIP-2
Burdelya et al., Mol Cancer Ther 2002 (Neoplasms) : Our results show that in vivo administration of AG-490 causes tumor cell apoptosis but does not inhibit IL-12 mediated macrophage activation and IFN-gamma production by lymphocytes
Okazawa et al., Am J Gastroenterol 2002 (Crohn Disease...) : The functional activity of IL- 12 and IL-18 was assessed by the effect of recombinant IL-12 and recombinant IL-18 on interferon-gamma production, the proliferative response, and the induction of IL-2R, IL-12R , and IL-18R of LPLs ... The functional activity of IL- 12 and IL-18 was assessed by the effect of recombinant IL-12 and recombinant IL-18 on interferon-gamma production, the proliferative response, and the induction of IL-2R, IL-12R , and IL-18R of LPLs ... The functional activity of IL- 12 and IL-18 was assessed by the effect of recombinant IL-12 and recombinant IL-18 on interferon-gamma production, the proliferative response, and the induction of IL-2R, IL-12R , and IL-18R of LPLs ... The functional activity of IL- 12 and IL-18 was assessed by the effect of recombinant IL-12 and recombinant IL-18 on interferon-gamma production, the proliferative response, and the induction of IL-2R , IL-12R, and IL-18R of LPLs
Hess et al., J Immunol 2003 (Carcinoma, Non-Small-Cell Lung...) : Cell depletion studies reveal that the IL-12 induced tumor suppression, IFN-gamma production, and the associated changes in gene expression are all dependent upon CD4+ T cells
Cleary et al., J Immunol 2003 (Immunologic Deficiency Syndromes...) : This mutation resulted in the absence of IL-12Rbeta1 protein on PBMC and an inability of T cells to specifically bind IL-12 or produce IFN-gamma in response to either IL-12 or IL-23
Kodama et al., Jpn J Pharmacol 2002 (Neoplasms, Experimental) : In this study using BALB/c mice in which a Th-2 response is genetically dominant, D-fraction reduced the expression of Th-2 cytokine interleukin (IL)-4 but markedly increased the expression of Th-1 cytokine interferon (IFN)-gamma in CD4 ( + ) T cells and also increased IL-12p70 production as well as IFN-gamma production by antigen presenting cells (APCs), suggesting that D-fraction promotes the differentiation into Th-1 cells of CD4 ( + ) T cells through enhancement of IL-12p70 production by APCs
Garnier et al., Immunol Lett 2003 : We also demonstrate by ELISA that rmIL-12 binds to CS B. Overall, our studies on this in vitro cellular model of the initiation of Th1 immune responses indicate a major role for cell-surface, iduronate-rich, CS proteoglycan in the biological activity of IL-12
Warrington et al., Blood 2003 : Costimulation of the T-cell and IL-12 receptors induced the transcription of CD28 in approximately 50 % of CD4 ( + ) CD28 ( null ) T-cell clones and lines ... IL-12 by itself did not restore CD28 expression
Perez et al., Blood 2003 (Burkitt Lymphoma...) : FL plus IL-15-driven ACF/CD56 ( + ) cells progressively expressed a mature NK functional program lysing both NK- and lymphokine-activate killer ( LAK ) -sensitive tumor targets and producing high levels of interferon-gamma (IFN-gamma), granulocyte-macrophage colony stimulating factor , tumor necrosis factor alpha, and IL-10 upon stimulation with IL-12 and IL-18 ... FL plus IL-15-driven ACF/CD56 ( + ) cells progressively expressed a mature NK functional program lysing both NK- and lymphokine-activate killer ( LAK ) -sensitive tumor targets and producing high levels of interferon-gamma (IFN-gamma) , granulocyte-macrophage colony stimulating factor, tumor necrosis factor alpha, and IL-10 upon stimulation with IL-12 and IL-18 ... FL plus IL-15-driven ACF/CD56 ( + ) cells progressively expressed a mature NK functional program lysing both NK- and lymphokine-activate killer ( LAK ) -sensitive tumor targets and producing high levels of interferon-gamma (IFN-gamma), granulocyte-macrophage colony stimulating factor, tumor necrosis factor alpha, and IL-10 upon stimulation with IL-12 and IL-18
Lettinga et al., Thorax 2003 (Legionnaires' Disease) : Patients released less IFN-gamma than controls in response to stimulation with LPS ( mean ( SE ) 393 ( 58 ) pg/ml v 914 ( 178 ) pg/ml ; p=0.001 ) and IL-12 ( 96 ( 14 ) pg/ml v 177 ( 41 ) pg/ml ; p=0.058 )
Foss et al., Viral Immunol 2002 (Porcine Reproductive and Respiratory Syndrome) : First, the principal observation was an enhancing effect of IL-12 on the interferon gamma response to PRRSV, with a more rapid and heightened PRRSV-specific interferon gamma ELISPOT response in peripheral blood mononuclear cells
Zenclussen et al., Am J Reprod Immunol 2002 (Abortion, Spontaneous) : We further investigated the effect of recombinant human ( rh ) IL-12 on the production of interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha in peripheral leukocytes ex vivo ... We further investigated the effect of recombinant human ( rh ) IL-12 on the production of interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha in peripheral leukocytes ex vivo
Malaguarnera et al., Eur Cytokine Netw 2002 (Malaria, Falciparum...) : IL-18 cooperates with IL-12 in the IFN-gamma production by T, B, and NK cells, and synergizes with IL-12 for IFN-gamma production by Th1 cells
Colpaert et al., Eur Cytokine Netw 2002 (Crohn Disease) : However, no effect of anti-IL-12 or anti-IFN-gamma on production of TNF , a cytokine with an important pathogenic role in Crohn 's disease, could be found
Sedlik et al., J Immunol 2003 : Importantly, Syk was also required for the induction of DC maturation and IL-12 production after FcR engagement, but not after engagement of other surface receptors, such as TNFR or Toll-like receptors
Maruyama et al., J Immunol 2003 : The induction of the IL-12 p40 gene is regulated by NF-kappaB in the presence of IFN-gamma ... The induction of the IL-12 p40 gene is regulated by NF-kappaB in the presence of IFN-gamma ... While IFN-gamma must be present for IL-12 p40 gene induction , the p35 gene is strongly induced by LPS, even in the absence of IFN-gamma, and therefore the induction of the p35 gene is IRF-1 independent
Torroella-Kouri et al., Int J Oncol 2003 (Adenocarcinoma...) : Our results suggest that tumor derived IL-11 may play a role in the depressed IL-12 production by macrophages, leading to the impaired immune functions observed during mammary tumorigenesis
Feng et al., Cell Res 2002 (Inflammation) : cAMP elevators inhibit LPS induced IL-12 p40 expression by interfering with phosphorylation of p38 MAPK in murine peritoneal macrophages ... cAMP elevators inhibit LPS induced IL-12 p40 expression by interfering with phosphorylation of p38 MAPK in murine peritoneal macrophages ... The present study examined a possible molecular mechanism involved in cAMP elevators induced inhibition of IL-12 p40 expression in response to LPS ... This is the first report that cAMP elevators inhibit LPS induced IL-12 production by a mechanism that is associated, at least in part, with p38 dependent inhibition by cAMP signaling pathways
Uno et al., Cancer Immunol Immunother 2003 (Neoplasm Metastasis...) : CD4 ( + ) and CD8 ( + ) T cell populations from an appreciable proportion of tumor bearing patients exhibited high levels of IL-12 responsiveness as evaluated by IL-12 stimulated interferon-gamma (IFN-gamma) production ... T cell IL-12 and IL-18 responsiveness is induced by TCR triggering and subsequent IL-12 stimulation respectively
Takahashi et al., J Pharmacol Exp Ther 2003 : In the same manner, salbutanol and terbutaline as well as norepinephrine, epinephrine, and isoproterenol regulated the IL-18 induced cytokine production, including IL-12 , tumor necrosis factor-alpha or interferon-gamma through the stimulation of beta 2-AR. Together with the previous finding that ICAM-1/lymphocyte function associated antigen-1 interaction plays a crucial role in the IL-18 initiated cytokine network, the present study strongly suggested that the stimulation of beta 2-AR inhibited the IL-18 activated cytokine cascade through the inhibitory effect on ICAM-1 expression, contributing to finding a new method for clinical treatment
Kokuho et al., Vet Immunol Immunopathol 2003 : An established clone ( 10B10 ) constitutively expressed chimeric IL-12Rs composed of intrinsic ( human ) beta1 and extrinsic ( porcine ) beta2 subunits, and produced interferon (IFN)-gamma in response to IL-12 of both species with optimal PHA/PMA stimulation
Monti et al., Transplantation 2003 : CD40 ligand induced production of both IL-12 and IL-10 was reduced in rapa-DC, and allogeneic T lymphocyte responses were moderately impaired when rapa-DC were used as stimulator cells
Pollara et al., J Infect Dis 2003 : LPS induced interleukin (IL)-12 secretion, and the induction of primary and secondary T cell responses were impaired by infection
Yamada et al., Autoimmunity 2002 (Disease Models, Animal...) : TRAb levels were greatest in mice immunized with both G2s and the TSHr in the presence of TL4, but not IL12
Koguchi et al., Am J Respir Crit Care Med 2003 (Sarcoidosis, Pulmonary...) : In vitro experiments showed that OPN production by peripheral blood mononuclear cells infected with Mycobacterium bovis bacillus Calmette-Guérin preceded the synthesis of IL-12 and interferon-gamma and that the neutralizing anti-OPN monoclonal antibody significantly reduced the production of IL-12 and interferon-gamma
Hou et al., J Immunol 2003 (MAP Kinase Signaling System...) : PTX-DC had also increased allostimulatory capacity and IL-12 and TNF-alpha production
Yu et al., J Immunol 2003 (HIV Infections) : TNF-alpha and RANKL cooperated with CD40L via differing mechanisms, i.e., TNF-alpha enhanced IL-12 production, whereas RANKL enhanced survival of CD40L stimulated DCs ... TNF-alpha and RANKL cooperated with CD40L via differing mechanisms, i.e., TNF-alpha enhanced IL-12 production, whereas RANKL enhanced survival of CD40L stimulated DCs
Bastos et al., J Interferon Cytokine Res 2002 (Malaria...) : Taken together, our results support the idea that the absence of IL-12p40 not only affects IFN-gamma production but also has deep consequences in macrophage effector functions that may contribute to exacerbation of the early phase of P. c. chabaudi malaria
Harada et al., J Interferon Cytokine Res 2002 : In addition, interleukin-12 p70 (IL-12p70) was induced by SCG in DBA/2 mice
Delgado et al., Biochem Biophys Res Commun 2003 : VIP was shown to inhibit IL-6, TNFalpha, IL-12 , chemokine, and nitric oxide production in endotoxin activated macrophages
Lee et al., J Immunol 2003 : IL-12 decreased the number of Fas ligand positive CD8 T cells and inhibited the activation of caspase-8 and caspase-3
Sugimoto et al., Eur J Immunol 2003 : IL-12 activates TYK2 and Janus kinase (JAK)-2 to induce the phosphorylation of various signal transducers and activators of transcription ( STAT ) proteins ... IL-12 activates TYK2 and Janus kinase (JAK)-2 to induce the phosphorylation of various signal transducers and activators of transcription ( STAT ) proteins ... Using two JAK inhibitors, tyrphostin A1 ( A1 ) for TYK2 and tyrphostin B42 ( B42 ) for JAK2, we investigated the involvement of JAK2 and TYK2 in IL-12 induced T cell proliferation and IFN-gamma production ... B42, but not A1, inhibited T cell proliferation along with down-regulation of IL-12 induced c-Myc expression and STAT5 phosphorylation ... IL-18, but not IL-12 , induced activator protein-1 (AP-1) responsible for high levels of IFN-gamma promoter activation
Nylén et al., Clin Exp Immunol 2003 : Previous studies have demonstrated that early production of interleukin (IL)-12 triggers IFN gamma secretion by NK cells
Vollstedt et al., J Exp Med 2003 (Herpes Simplex...) : This resistance required interferon (IFN)-alpha/beta for viral and interleukin (IL)-12 for bacterial infections ... This resistance required interferon (IFN)-alpha/beta for viral and interleukin (IL)-12 for bacterial infections
Mackey et al., Eur J Immunol 2003 : Distinct contributions of different CD40 TRAF binding sites to CD154 induced dendritic cell maturation and IL-12 secretion
Samten et al., J Immunol 2003 : CD40LT induced IFN-gamma production was dependent on production of IL-12 and IL-18, but did not require IL-15
Bruna-Romero et al., J Immunol 2003 (Malaria) : DC-CK1 appears to act preferentially on naive mouse lymphocytes, and its adjuvant effect requires IL-12 , but not IFN-gamma or CD40
Kawashima et al., Arthritis Rheum 2003 (Arthritis, Rheumatoid) : IL-1 beta and, less effectively, IL-12 could induce RA fibroblast-like synoviocytes to produce IL-6 , but IL-18 failed to have an effect ... Total RA synovium cells containing T cells showed a strong expression of both IL-18R alpha and IL-18R beta mRNA, and only IL-18R beta was up-regulated by IL-12 ... IL-12 induced IFN gamma production by total RA synovium cells containing T cells was increased by additional IL-18 and decreased by IL-18BP
Bream et al., Blood 2003 : IL-4 synergistically enhances both IL-2- and IL-12 induced IFN-gamma expression in murine NK cells
Guo et al., J Biol Chem 2003 (Inflammation) : Expression of SBR-p38alpha in primary T lymphocytes abrogated the ability of SB 203580 to inhibit the production of interferon-gamma induced by co-ligation of CD3 and CD28 but not the production of interferon-gamma or IL-10 induced by IL-12 ... Expression of SBR-p38alpha in primary T lymphocytes abrogated the ability of SB 203580 to inhibit the production of interferon-gamma induced by co-ligation of CD3 and CD28 but not the production of interferon-gamma or IL-10 induced by IL-12
Kikawada et al., J Immunol 2003 (IgG Deficiency...) : IL-12 deficiency in MRL-Fas ( lpr ) mice delays nephritis and intrarenal IFN-gamma expression, and diminishes systemic pathology
Wu et al., J Neuroimmunol 2003 (Encephalomyelitis, Autoimmune, Experimental...) : The IL-12R beta 2 expression on Th1 cells from draining LNC was increased in the presence of IL-12 and IFN-gamma
Stuyt et al., Cytokine 2003 : Blocking endogenous IL-12 and TNF reduced IFN-gamma production by 69 and 36 %
Faveeuw et al., Eur J Immunol 2003 : In the present study, we show that PGD ( 2 ) inhibits , in a dose dependent manner, the CD40- and LPS induced secretion of the Th1 driving factor IL-12 by murine splenic dendritic cells ( DC ), the most potent antigen presenting cells ... In the present study, we show that PGD ( 2 ) inhibits, in a dose dependent manner, the CD40- and LPS induced secretion of the Th1 driving factor IL-12 by murine splenic dendritic cells ( DC ), the most potent antigen presenting cells ... Together these data suggest that PGD ( 2 ), by interacting with DP1 and by binding to other target cellular proteins, may regulate immune responses by affecting IL-12 production in DC
Krajina et al., Eur J Immunol 2003 (Colitis...) : cLP DC from non transplanted ( healthy ) and transplanted ( diseased ) mice produce similar amounts of IL-12 p70 and IL-10 in response to CD40 signaling, but the inducible IL-12 p40 release is 5-15-fold higher in mice with colitis than in non transplanted mice
Brown et al., Pediatr Allergy Immunol 2003 : Production of IL-12 was increased in the vaginally delivered group in response to LPS and PHA but not to ConA/PMA
Heinzelmann et al., Int J Surg Investig 2001 : HBP increased the LPS induced production of IL-8, MIP-1alpha, TNF-alpha, IL-1beta, but HBP did not increase the significant LPS induced release of IL-10, monocyte chemoattractant protein MCP-1, and IL- 12 ... HBP increased the LPS induced production of IL-8, MIP-1alpha, TNF-alpha, IL-1beta, but HBP did not increase the significant LPS induced release of IL-10, monocyte chemoattractant protein MCP-1, and IL- 12
Dauer et al., J Immunol 2003 (Second Messenger Systems) : FastDC expressed mature DC surface markers as well as chemokine receptor 7 and secreted IL-12 ( p70 ) upon CD40 ligation in the presence of IFN-gamma
Muraille et al., J Immunol 2003 (Genetic Predisposition to Disease...) : Toll-like receptors recognize pathogen associated molecular patterns and are crucially implicated in the induction of IL-12 secretion by APC
Komatsu et al., Free radical research 2003 : It was found that the glutathione reductase inhibitor, 1,3-bis ( 2-chloroethyl ) -1-nitrosourea, markedly augmented LPS induced IL-12p40 production particularly when it was added for 24 h before LPS stimulation, whereas the glutathione-synthesis inhibitor, L-buthionine- ( S, R ) -sulfoximine, suppressed IL-12p40 production
Matsuse et al., Int Immunopharmacol 2003 (Asthma...) : In contrast, intranasal IL-12 significantly increased IFN-gamma production in the thoracic lymph node cultures and decreased the IL-5/IFN-gamma ratio, suggesting a Th2-Th1 shift
Schierack et al., Infect Immun 2003 : Whereas filarial cystatins markedly increased the production of interleukin (IL)-10, C. elegans cystatins increased the production of IL-12 and gamma interferon ( IFN-gamma ) by human PBMC
Chakir et al., Microbes Infect 2003 (Disease Susceptibility...) : Previous studies utilizing mice deficient for either the IL-12 cytokine or the IL-12 induced signaling molecule STAT4 have established a critical role for IL-12 during infection with Leishmania major
Kang et al., Eur J Pharmacol 2003 : Inhibitors of p38 MAPK prevented the stimulatory effects of alpha ( 2 ) -adrenoceptor agonists on IL-12 production
Ito et al., J Immunol 2003 (Encephalomyelitis, Autoimmune, Experimental) : MOG-specific T cells stimulated with anti-CD3 and anti-CD28 in the presence of IL-12 or IL-18 alone transferred only mild experimental autoimmune encephalomyelitis ( EAE ) into a low percentage of recipients
Nishioka et al., J Leukoc Biol 2003 (Lymphoma, T-Cell...) : We examined the mechanisms involved in interleukin (IL)-12 mediated suppression of cellular immunity in mice using allogeneic mixed leukocyte reaction (MLR) stimulated by dendritic cells (DCs) in vitro and compared the effect of IL-12 on MLR in mice and humans ... Although IL-12 stimulated human MLR , the addition of IL-12 or interferon-gamma (IFN-gamma) resulted in a dose dependent suppression of MLR in mice
Kamath et al., Proc Natl Acad Sci U S A 2003 : Such priming results also in a nonmemory response to whole bacteria and to lipopolysaccharide, characterized by IL-12 dependent secretion of IFN-gamma by gammadelta T cells and by gammadelta T cell proliferation
Kim et al., Ann N Y Acad Sci 2003 : We observed that necrotic cells induced IL-12 p40 expression, whereas phagocytosis of apoptotic cells profoundly reduced IL-12 production from stimulated macrophages
Takeda et al., J Immunol 2003 : Although IL-27 did not directly induce IFN-gamma production by wild-type CD4 ( + ) T cells, IL-12 dependent IFN-gamma production was augmented by IL-27 stimulation in wild-type naive CD4 ( + ) T cells but was impaired in WSX-1-deficient naive CD4 ( + ) T cells
Gosset et al., J Immunol 2003 : Moreover, PGD ( 2 ) and 15d-PGJ ( 2 ) strongly reduce the secretion of the Th1 promoting cytokine IL-12 and affect the synthesis of chemokines involved in Th1 cell chemotaxis, particularly CXCL10
Weinstock et al., J Immunol 2003 : IL-12 and IL-18 strongly induce NK-1R mRNA expression in splenocytes from Stat4 ( -/- ) mice, suggesting that the Stat4 pathway was not required for the induction of NK-1R transcription ... Splenic T cells exposed to IL-12 or IL-18 in the presence of IL-10 expressed no NK-1R mRNA ... Thus, IL-12 and IL-18 induce T cells to express NK-1R through NF-kappa B activation
Schröder et al., J Immunol 2003 : However, the resulting hyporesponsive states differ regarding their reversibility by the IFN-gamma inducing cytokine IL-12 ... In an in vitro IL-12/IL-18 responsiveness model, we demonstrated that IL-10 , if permanently present, does not directly inhibit IL-12/IL-18 responsiveness in T/NK cells but indirectly interferes with IFN-gamma production in the presence of monocytes
La Pine et al., Pediatr Res 2003 (Streptococcal Infections) : Cord blood mixed mononuclear cells ' production of IFN-gamma is enhanced by added recombinant IL-18 and IL-12
Stassi et al., New Microbiol 2003 (Inflammation) : Our results demonstrate that live H. pylori does not induce IL-4 release whereas it stimulates MCP-1 and IL-12 production
Jefford et al., Blood 2003 (Melanoma) : They responded vigorously to CD40L or E coli, expressing cytokines known to regulate interferon-gamma (IFN-gamma) in T cells ( IL-12p70 , IL-18, and IL-23 ), but required prostaglandin E2 ( PGE2 ) during stimulation to migrate to chemokines
Yasukawa et al., Nat Immunol 2003 : In macrophages lacking the Socs3 gene or carrying a mutation of the SOCS3 binding site in gp130, the lipopolysaccharide induced production of tumor necrosis factor (TNF) and IL-12 is suppressed by both IL-10 and IL-6 ... In macrophages lacking the Socs3 gene or carrying a mutation of the SOCS3 binding site in gp130, the lipopolysaccharide induced production of tumor necrosis factor (TNF) and IL-12 is suppressed by both IL-10 and IL-6
Petro et al., Int Immunopharmacol 2003 : Stimulation of IL-12 production by smokeless tobacco extract (STE) could increase the chances of oral inflammatory disease
Aliberti et al., J Immunol 2003 (Disease Models, Animal...) : In the present work, we demonstrate that kinins mobilize dendritic cells to produce IL-12 through activation of the B(2) bradykinin receptor subtype and that bradykinin induced IL-12 responses are tightly regulated both by angiotensin converting enzyme, a kinin degrading peptidase, and by endogenous IL-10
Strengell et al., J Immunol 2003 : We have previously shown that IL-15 and IL-21 induce the expression of IFN-gamma, T-bet, IL-12R beta 2, and IL-18R genes both in NK and T cells ... We have previously shown that IL-15 and IL-21 induce the expression of IFN-gamma, T-bet, IL-12R beta 2, and IL-18R genes both in NK and T cells
Trembleau et al., J Immunol 2003 (Diabetes Mellitus, Type 1...) : IL-12 administration accelerates autoimmune diabetes in both wild-type and IFN-gamma-deficient nonobese diabetic mice, revealing pathogenic and protective effects of IL-12 induced IFN-gamma ... Here we show that IL-12 induced IFN-gamma production is dispensable for diabetes acceleration, because exogenous IL-12 could enhance IDDM development in IFN-gamma-deficient as well as in IFN-gamma-sufficient NOD mice ... Surprisingly, IL-12 induced IFN-gamma hinders pancreatic B cell infiltration and inhibits the capacity of APCs to activate T cells ... NO, an effector molecule in beta cell destruction, is produced ex vivo in high quantity by pancreas infiltrating cells through a mechanism involving IL-12 induced IFN-gamma
Qi et al., Infect Immun 2003 : Further, these stimuli differentially conditioned CD40 dependent IL-10 and IL-12 production by DCs
Weigt et al., Immunobiology 2003 : Furthermore, both substances decreased the endocytotic capacity of DC and induced the release of bioactive TNF-alpha and IL-10, whereas LPS additionally increased IL-12 release
Huber et al., Int Immunopharmacol 2003 : When delivered i.n., IL-12 induces less systemic IFN-gamma production and fewer pathological tissue changes, yet is efficacious, as indicated by enhanced CD3 ( + ) T cell activation and increased production of Th1 associated immunoglobulins ( i.e., serum IgG2a )
Lenert et al., J Clin Immunol 2003 (Disease Models, Animal...) : Neutralizing anti-IL-10 antibodies almost completely restored PN IL-6 and IL-12p40 secretion to DBA-1 levels, whereas exogenous IL-10 inhibited in vitro IL-6 and IL-12p40 production in DBA-1 mice
Puccetti et al., Crit Rev Immunol 2002 : However, although both cytokines enhance the Th1 costimulatory functions of APCs, and IL-23 does induce IL-12 from APCs, their effects, which in part overlap, can be differentiated from one another
Heystek et al., Clin Immunol 2003 : We demonstrate that IFNalpha/beta enhance IL-12p70 production by immature DCs but inhibit IL-12p70 production by mature DCs
Goodridge et al., Immunology 2003 (MAP Kinase Signaling System) : Such Erk MAPkinase activation is downstream of calcium influx and targets LPS induced IL-12 p40 transcription by suppressing the synthesis of the transcription factor, interferon regulatory factor-1 (IRF-1)
Chakir et al., Eur J Immunol 2003 : Furthermore, anti-CD3 induced IL-12/IL-18 responsiveness was fully abrogated in the presence of cyclosporin A whereas IL-2 induced IL-12/IL-18 responsiveness was not, reminiscent of the previously reported IL-12+IL-18 innate pathway of T cell activation
Kawakami et al., Rev Iberoam Micol 2002 : IL-12 and IL-18 activate the synthesis of IFN-gamma by innate immune cells, including NK, NKT and gamma delta T cells and promote the differentiation of Th1-type acquired immune responses
Xia et al., Scand J Immunol 2003 : In addition, inhibition of ERK by PD98059 significantly suppressed IL-10 and increased the IL-12 production ... The above findings suggest a unidirectional negative autocrine regulation of IL-12 by IL-10 in activated DCs and that activation of ERK involves the differential production of IL-10 and IL-12 by activated DCs ... The above findings suggest a unidirectional negative autocrine regulation of IL-12 by IL-10 in activated DCs and that activation of ERK involves the differential production of IL-10 and IL-12 by activated DCs ... The above findings suggest a unidirectional negative autocrine regulation of IL-12 by IL-10 in activated DCs and that activation of ERK involves the differential production of IL-10 and IL-12 by activated DCs
Morichika et al., Transplantation 2003 : Whereas IL-18 significantly induced the expression of ICAM-1, B7.1, and B7.2 on monocytes in MLR and the production of interferon-gamma and IL-12 , PGE2 inhibited these IL-18 initiated enhancements
Nunnari et al., Ann Intern Med 2003 (Acquired Immunodeficiency Syndrome...) : During follow-up, IL-2 and IL-12 levels decreased significantly ( P = 0.005 and P = 0.01, respectively ) and IL-4 and IL-10 levels increased significantly ( P = 0.01 and P = 0.004, respectively ) in the GBV-C negative group but did not change substantially in the GBV-C positive group ... During follow-up, IL-2 and IL-12 levels decreased significantly ( P = 0.005 and P = 0.01, respectively ) and IL-4 and IL-10 levels increased significantly ( P = 0.01 and P = 0.004, respectively ) in the GBV-C negative group but did not change substantially in the GBV-C positive group
Hayashi et al., J Comp Pathol 2003 (Autoimmune Diseases...) : Interleukin (IL)-12 in the presence of IL-18 induces T helper ( Th ) 1 dominated systemic immune reactions, such as those that occur in autoimmune insulitis in suckling mice infected with reovirus type-2 ( Reo-2 )
Utsugi et al., J Immunol 2003 (MAP Kinase Signaling System) : Inhibition of p38 MAP kinase activation using SB203580 dose dependently repressed LPS induced IL-12 p40 production, as described ... c-Jun N-terminal kinase negatively regulates lipopolysaccharide induced IL-12 production in human macrophages : role of mitogen activated protein kinase in glutathione redox regulation of IL-12 production ... Inhibition of p38 MAP kinase activation using SB203580 dose dependently repressed LPS induced IL-12 p40 production, as described ... Conversely, inhibition of JNK activation using SP600125 dose dependently enhanced both LPS induced IL-12 p40 production from THP-1 cells and p70 production from human monocytes ... Finally, the increase in the ratio of GSH/GSSG induced by glutathione reduced form ethyl ester ( GSH-OEt ) dose dependently enhanced LPS induced IL-12 p40 production in PMA treated THP-1 cells ... Our findings indicate that JNK negatively affects LPS induced IL-12 production from human macrophages, and that glutathione redox regulates LPS induced IL-12 production through the opposite control of JNK and p38 MAP kinase activation ... The present study uses human macrophages to examine whether the JNK pathway is required for LPS induced IL-12 production and defines how JNK is involved in the regulation of IL-12 production by glutathione redox, which is the balance between intracellular reduced ( GSH ) and oxidized glutathione ( GSSG )
Martin et al., J Immunol 2003 (MAP Kinase Signaling System) : Furthermore, the ability of the PI3K-Akt pathway to modulate IL-10 and IL-12 production appears to be mediated by the selective suppression of extracellular signal regulated kinase 1/2 activity, as the MEK1 inhibitor PD98059 closely mimicked the effects of wortmannin and LY294002 to differentially regulate IL-10 and IL-12 production by P. gingivalis LPS stimulated monocytes
Cattaruzza et al., J Biol Chem 2003 : One functional consequence of IL-10 up-regulation of NOS-3 abundance in cultured endothelial cells is the attenuation of CD154 induced IL-12 p40 expression ... Moreover, CD154 induced IL-12 p40 expression is enhanced after blockade of NOS-3 activity but attenuated in the presence of exogenous nitric oxide
Sher et al., Immunol Res 2003 (Disease Models, Animal...) : In addition, we present an overview of our studies investigating the mechanisms by which the IFN-gamma produced as a consequence of IL-12 stimulation controls intracellular replication of the parasite in host cells
Fei et al., Atherosclerosis 2003 (Arteriosclerosis) : We here demonstrate that IL-12 induces enhanced IFN-gamma secretion in peripheral blood mononuclear cells ( PBMC ), with no addition of a specific antigen, as determined by ELISPOT
Hu et al., Di Yi Jun Yi Da Xue Xue Bao 2003 : After rAAV-HBsAg infection, the IL-12 secretion of DCs was significantly enhanced ( P < 0.05 ), while IFN-gamma production by the lymphocyte populations co-cultured with rAAV-HbsAg infected DCs was reduced ( P < 0.01 )
Murray et al., J Infect Dis 2003 (Disease Models, Animal...) : As judged by IL-10R blockade, endogenous IL-10 primarily regulates killing in L. donovani infection by suppressing production of and responses to the Th1 cell-type cytokines, IL-12 , and IFN-gamma
Doxsee et al., J Immunol 2003 : The immune response modifier and Toll-like receptor 7 agonist S-27609 selectively induces IL-12 and TNF-alpha production in CD11c+CD11b+CD8- dendritic cells ... In contrast, stimulation through TLR4 and TLR9 induced IL-12 production predominantly from CD8 ( + ) DCs, consistent with previously published data ... In contrast, stimulation through TLR4 and TLR9 induced IL-12 production predominantly from CD8 ( + ) DCs, consistent with previously published data
Yoshimoto et al., J Immunol 2003 : Ectopic expression of dominant negative SPHK2 in Th1 cell clone significantly reduced IL-12 induced IFN-gamma production, while that of wild-type SPHK2 enhanced it ... A similar decrease in IL-12 induced IFN-gamma production was observed when dominant negative SPHK2 was expressed in activated primary T cells using a retroviral expression system
Huang et al., J Immunol 2003 : Our results show that HKBA mediated induction of IL-12p40 and TNF is dependent on the adapter molecule MyD88
Zhu et al., J Biol Chem 2003 : However, molecular mechanisms through which interferon (IFN)-gamma and bacterial products synergistically activate IL-12 p40 gene expression are less clear ... A dominant negative NFAT molecule attenuates LPS- and IFN-gamma activated endogenous IL-12 p40 mRNA expression
Ghoreschi et al., J Mol Med (Berl) 2003 (Psoriasis) : After binding to their receptors both IL-12 and IFN-gamma promote intracellular IFN-gamma production by activating signal transducer and activator of transcription ( STAT ) 4 or 1
Haddad et al., J Exp Med 2003 : These data suggest that IL-12 induced functional PSGL-1 expression is a major determinant for the recruitment of Th1 effector cells to noninflamed as well as inflamed intestine
Croy et al., J Reprod Immunol 2003 : Here, we review recent studies showing that IL-15 is the critical cytokine controlling uNK cell differentiation and that uNK cells are activated by either IL-12 or IL-18 and by other factors when both IL-12 and IL-18 are genetically absent from implantation sites
Nold et al., Biochem Pharmacol 2003 : Notably, IL-18 BPa : Fc ( 200 ng/mL ) further reinforced dexamethasone ( 5 nM ) - or mycophenolic acid ( 2 microM ) -mediated reduction of LPS/IL-12 induced IFNgamma production by an additional 50.5 or 49.9 %, respectively
Calvani et al., Kidney Int 2003 (Hypergammaglobulinemia...) : Interleukin-12 (IL-12) is essential for Th1 cell differentiation and induces interferon-gamma (IFN-gamma) production
Hoey et al., EMBO J 2003 : However, Stat4alpha is required for normal levels of IL-12 induced interferon-gamma production from Th1 cells
Malmgaard et al., J Gen Virol 2003 (Herpes Simplex) : Interferon (IFN)-alpha/beta, interleukin (IL)-12 and IL-18 coordinately induce production of IFN-gamma during infection with herpes simplex virus type 2 ... IL-12 alone induced the most expression of IFN-gamma , which was augmented by cotreatment with IFN-alpha or IL-18, or combinations of IFN-alpha and IL-18
Kalthoff et al., Clin Exp Immunol 2003 : In contrast, pimecrolimus did not inhibit the LPS induced secretion of IL-12 , surface expression of costimulatory molecules or the maturation of M-DC into potent stimulators of T cells
Tuyaerts et al., Cancer Gene Ther 2003 : We document that CD40 ligation leads to a significant production of IL-12 by the electroporated DC, although the level of IL-12 production is somewhat lower than for non- or mock electroporated DC
Ferlazzo et al., Int Immunopharmacol 2003 : In addition, ANX-1 stimulated IL-10 release may also be responsible for the inhibition of IL-12 mRNA expression and, consequently, IL-12 synthesis
Onaitis et al., Surgery 2003 (Melanoma) : CD40L augments DC IL-12 secretion and is essential to potentiate specific antimelanoma cytolytic responses stimulated by the Mart-1 antigen
Watford et al., Cytokine Growth Factor Rev 2003 : IL-12 induces interferon-gamma (IFN-gamma) production by NK, T cells, dendritic cells ( DC ), and macrophages
Schuerwegh et al., Cytometry B Clin Cytom 2003 (Arthritis, Rheumatoid) : There was a strong linear correlation between extracellular quantitative ( pg/ml ) and intracellular semiquantitative detection of LPS stimulated IL-1beta, IL-6, IL-10, and IL-12 production ( r > 0.9 )
Gately et al., Cell Immunol 1992 : However, IL-12- and IL-2 induced LAK cell activation could be partially inhibited by anti-human TNF-alpha
Perussia et al., J Immunol 1992 : In the case of phorbol-diester induced proliferation, the effect of IL-12 is in part mediated by induced IL-2 production, as suggested by the observation that IL-12 enhances IL-2 production in these cultures and that anti-IL-2 antibodies inhibit proliferation ... This inhibitory effect is, at least in part, indirect, and depends on IL-12 induced production of TNF
Trinchieri et al., Prog Growth Factor Res 1992 (Acquired Immunodeficiency Syndrome...) : NKSF/IL-12 induces T and NK cells to produce IFN-gamma and synergizes with other IFN-gamma inducers in this effect ... In vitro, and probably in vivo, NKSF/IL-12 is required for optimal IFN-gamma production ... IFN-gamma, a product of Th1 cells, enhances NKSF/IL-12 production by mononuclear cells, whereas IL-10 and IL-4, products of Th2 cells, efficiently inhibit it ... IFN-gamma, a product of Th1 cells, enhances NKSF/IL-12 production by mononuclear cells, whereas IL-10 and IL-4 , products of Th2 cells, efficiently inhibit it ... IFN-gamma , a product of Th1 cells, enhances NKSF/IL-12 production by mononuclear cells, whereas IL-10 and IL-4, products of Th2 cells, efficiently inhibit it
Hartmann et al., Int J Parasitol 2003 (Nematode Infections) : Caenorhabditis elegans cystatins induce the production of the Th1 cytokine IL-12 , in contrast to filarial cystatins that upregulate IL-10
Naume et al., J Immunol 1992 : Thus, IL-12 can directly influence NK cell activities in purified CD56+ cells, and endogenously produced TNF-alpha is involved in mediating the effects of both IL-12 and IL-7
DePaolo et al., J Immunol 2003 : CC chemokine ligand 2 and its receptor regulate mucosal production of IL-12 and TGF-beta in high dose oral tolerance
Weinstock et al., J Immunol 2003 (Chronic Disease...) : Experiments showed that IL-12 induced NK-1R transcription in CD4 ( + ) T cells cultured in vitro
Sugita et al., Am J Reprod Immunol 2003 : The effects of G-CSF on interleukin (IL)-2 and/or IL-12 induced interferon (IFN)-gamma production of magnetic cell sorting (MACS) sorted decidual lymphocytes was examined by enzyme linked immunosorbent spot forming cell assay ( ELISPOT )
Pfaff et al., Parasite Immunol 2003 (Entamoebiasis...) : Although having no effect on IL-10, IL-12 strongly promoted spontaneous and parasite antigen-driven IFN-gamma production by PBMC, whereas IL-18 was only moderately affecting IFN-gamma release by PBMC re-stimulated with E. histolytica- or O. volvulus-specific antigens ... This study disclosed that IL-18 will significantly down-regulate parasite-specific IL-10 production, whereas IL-12 induced IFN-gamma and inhibited IL-13 production by PBMC from humans exposed to O. volvulus and E. histolytica ... This study disclosed that IL-18 will significantly down-regulate parasite-specific IL-10 production, whereas IL-12 induced IFN-gamma and inhibited IL-13 production by PBMC from humans exposed to O. volvulus and E. histolytica
Matsuura et al., Eur J Biochem 2003 : Stimulation of the PEC culture with IL-12 or IL-18 induced production of IFN-gamma and NO, and these cytokines, in combination, exhibited marked synergism
Emoto et al., J Immunol 2003 (Listeriosis) : We assume that highly biased type 1 immune responses in LFA-1 ( -/- ) mice are caused by increased levels of IL-12 from granulocytes and that granulocytes play a major role in IFN-gamma secretion by NK cells
Kang et al., Planta Med 2003 : Baicalein potently inhibited the LPS induced IL-12 production from both primary macrophages and RAW264.7 monocytic cell-line in a dose dependent manner ( the IC50 values were 43.7 and 17.4 microM, respectively )
Kikuchi et al., Immunol Lett 2003 : Differential role of mitogen activated protein kinases in CD40 mediated IL-12 production by immature and mature dendritic cells ... Using a murine spleen derived dendritic cell ( DC ) line ( BC1 ) CD40 mediated interleukin (IL)-12 production was analyzed and compared between immature and mature DC ... It seems that roles of ERK and p38 MAPK for IL-12 production are developmentally changed in murine DC ... It seems that roles of ERK and p38 MAPK for IL-12 production are developmentally changed in murine DC
Takahashi et al., J Pharmacol Exp Ther 2003 : Moreover, PGE1 inhibited the production of IL-12 and interferon-gamma in PBMC in the presence and absence of IL-18 , whereas PGE1 induced IL-10 production
Mocikat et al., Immunity 2003 (Carcinoma...) : The activated NK cells then prime DC to produce IL-12 and to induce highly protective CD8 T cell memory responses
Chieppa et al., J Immunol 2003 (Inflammation) : MUC III ( a partially purified mucin ), biglycan ( a purified complex proteoglycan ), and mannosylated lipoarabinomannan from Mycobacterium tuberculosis affected cytokine production with high IL-10, IL-1R antagonist, IL-1R type II, and inhibition of IL-12 ... MUC III ( a partially purified mucin ), biglycan ( a purified complex proteoglycan ), and mannosylated lipoarabinomannan from Mycobacterium tuberculosis affected cytokine production with high IL-10, IL-1R antagonist, IL-1R type II, and inhibition of IL-12
Liu et al., J Exp Med 2003 : We demonstrate that the levels of IL-12 p35 protein stimulated by IFN-gamma and lipopolysaccharide (LPS) correspond to those of its mRNA, and that the nuclear factor kappaB signaling pathway is essential for the induction of IL-12 p35 transcription by LPS ... We demonstrate that the levels of IL-12 p35 protein stimulated by IFN-gamma and lipopolysaccharide (LPS) correspond to those of its mRNA, and that the nuclear factor kappaB signaling pathway is essential for the induction of IL-12 p35 transcription by LPS ... We demonstrate that the levels of IL-12 p35 protein stimulated by IFN-gamma and lipopolysaccharide (LPS) correspond to those of its mRNA, and that the nuclear factor kappaB signaling pathway is essential for the induction of IL-12 p35 transcription by LPS ... IRF-1 plays a major role in the transcriptional activation of the IL-12 p35 gene, but not of the p40 gene, by physically interacting with an inverted IRF element within the IL-12 p35 promoter upon IFN-gamma activation ... Thus, IRF-1 acts as a critical component of IFN-gamma signaling in the selective activation of IL-12 p35 transcription in synergy with LPS mediated events ... Thus, IRF-1 acts as a critical component of IFN-gamma signaling in the selective activation of IL-12 p35 transcription in synergy with LPS mediated events
Freudenberg et al., J Endotoxin Res 2003 (Hypersensitivity) : IL-12 and IL-18 are intimately involved in the induction of IFN-gamma by bacteria
Lehtonen et al., Cytokine 2003 : IFN-alpha and IL-12 activate IFN regulatory factor 1 (IRF-1), IRF-4, and IRF-8 gene expression in human NK and T cells ... IFN-alpha and IL-12 activate IFN regulatory factor 1 (IRF-1) , IRF-4, and IRF-8 gene expression in human NK and T cells ... IFN-alpha and IL-12 activate IFN regulatory factor 1 (IRF-1), IRF-4 , and IRF-8 gene expression in human NK and T cells ... Both IFN-alpha and IL-12 strongly up-regulated IRF-1, IRF-4 , and IRF-8 mRNA and protein expression ... Both IFN-alpha and IL-12 strongly up-regulated IRF-1 , IRF-4, and IRF-8 mRNA and protein expression ... Both IFN-alpha and IL-12 strongly up-regulated IRF-1, IRF-4 , and IRF-8 mRNA and protein expression
Wan et al., Blood 2004 : The induction of interferon-gamma-inducible protein 10 ( IP-10 ) secretion by Hsp70L1 is not shared by Hsp70, and other functional differences include more potent stimulation of DC IL-12p70 , CC-chemokine, and CCR7 and CXCR4 expression by Hsp70L1. Immunization of mice with the hybrid peptide Hsp70L1-ovalbumin ( OVA ) ( 257-264 ) induces an OVA ( 257-264 ) -specific Th1 response and cytotoxic T lymphocyte ( CTL ) that results in significant inhibition of E.G7-OVA tumor growth ... The induction of interferon-gamma-inducible protein 10 ( IP-10 ) secretion by Hsp70L1 is not shared by Hsp70, and other functional differences include more potent stimulation of DC IL-12p70 , CC-chemokine, and CCR7 and CXCR4 expression by Hsp70L1. Immunization of mice with the hybrid peptide Hsp70L1-ovalbumin ( OVA ) ( 257-264 ) induces an OVA ( 257-264 ) -specific Th1 response and cytotoxic T lymphocyte ( CTL ) that results in significant inhibition of E.G7-OVA tumor growth
Maestroni et al., J Neuroimmunol 2003 (Dermatitis, Contact) : Inhibition of IL-12 is mediated by both beta2- and alpha2(A)-ARs , while stimulation of IL-10 by beta2-ARs only
Rajavelu et al., Clin Diagn Lab Immunol 2003 (Tuberculosis) : The standard PPD and heat killed H37Rv antigens induced the maximum levels of T-cell proliferation and IFN-gamma secretion but low levels of IL-12
Inamura et al., Immunol Lett 2003 (Liver Cirrhosis) : Although the number of IEL was significantly increased in the cirrhosis group, their proliferative response was decreased, and IFN-gamma production from each IEL was markedly diminished in either stimulation by anti-CD3 or IL-12/IL-18
Une et al., Clin Exp Immunol 2003 (Chagas Disease) : Role of IFN-alpha/beta and IL-12 in the activation of natural killer cells and interferon-gamma production during experimental infection with Trypanosoma cruzi
Berg et al., J Exp Med 2003 (Listeriosis) : Thus, CD8+ T cells play an important role in the innate immune response against intracellular pathogens by rapidly secreting IFN-gamma in response to IL-12 and IL-18
Schwacha et al., Shock 2003 (Burns) : Macrophages isolated from injured mice produced higher levels of PGE2, TNF-alpha, IL-6, and IL-10 and lower levels of IL-12 in response to LPS stimulation than did cells from sham treated mice
Kuroki et al., Cancer Immunol Immunother 2003 : These results indicate that uptake of OK-432 by imMo-DCs is an early critical event for IL-12 p70 production and that NF-kappaB activation induced by OK-432 also contributes partially to IL-12 p70 production
Exley et al., Immunology 2003 (Cardiovirus Infections...) : EMCV-D resistance depends on IL-12 mediated interferon-gamma (IFN-gamma) production
Morin et al., Clin Exp Immunol 2003 (Diabetes Mellitus) : As we shown previously, NOD BM-DC exhibit an enhanced capacity to produce IL-12p70 in response to lipopolysaccharide (LPS) and anti-CD40 stimulation compared to BM-DC from control mice ... As we shown previously, NOD BM-DC exhibit an enhanced capacity to produce IL-12p70 in response to lipopolysaccharide (LPS) and anti-CD40 stimulation compared to BM-DC from control mice
Hoeve et al., Eur J Immunol 2003 : We have previously shown that human IL-12Rbeta1-deficiency leads to impaired IL-12 responsiveness and unusual susceptibility to infections due to mycobacteria and salmonellae ... Thus, IL-12Rbeta1-deficiency may impair both IL-12- and IL-23 signaling, and both may contribute to the immunological phenotypes
Brady et al., Eur J Immunol 2003 (Hepatitis C, Chronic) : In addition, NS4 inhibited IL-12 production by PBMC in response to LPS and IFN-gamma , and Th1 responses to recall antigens in normal individuals ... In addition, NS4 inhibited IL-12 production by PBMC in response to LPS and IFN-gamma, and Th1 responses to recall antigens in normal individuals ... Our data suggest that HCV subverts cellular immunity by inducing IL-10 and inhibiting IL-12 production by monocytes, which in turn inhibits the activation of DC that drive the differentiation of Th1 cells
Park et al., Biochem Biophys Res Commun 2003 : However, polymyxin B ( PB ; an LPS inhibitor ) effectively inhibited the surface molecules and IL-12 production induced by LPS , but had no effect on the PL in DC ... Moreover, the inhibitors of protein tyrosine kinase ( PTK ) or protein kinase C ( PKC ) significantly blocked the expression of surface molecules and IL-12 production in PL-stimulated DC
Aniansson Zdolsek et al., Pediatr Allergy Immunol 2003 (Hypersensitivity, Immediate) : Reduced IL-2 induced IL-12 responsiveness in atopic children ... This was accompanied by a low IL-2- and IL-12 induced IFN-gamma production, possibly reflecting the reduced capacity of atopic children to up-regulate the IL-12 receptor
Makarenkova et al., J Neuroimmunol 2003 (Lung Neoplasms) : BOM and GRP also inhibited interleukin-12 (IL-12) production by DC and their ability to activate T cells
Lucas et al., Proc Natl Acad Sci U S A 2003 : IL-27 regulates IL-12 responsiveness of naive CD4+ T cells through Stat1 dependent and -independent mechanisms
Liebau et al., Cytokines Cell Mol Ther 2002 (Osteosarcoma) : ICAM-1 expression on HOS cells was significantly enhanced by IL-12 , but only when co-cultured in cell-to-cell contact with peripheral lymphocytes ... If HOS cells and peripheral lymphocytes were separated in co-cultures, IL-18 could substitute for cell-to-cell contact, facilitating IL-12 mediated enhancement of ICAM-1 ... These findings demonstrate that IL-12 can enhance the expression of ICAM-1 in the presence of IFN-gamma and, with IL-18, enhances NK anti-tumor activity
Fonseca et al., Vaccine 2004 (Granuloma...) : In an attempt to determine the role of IFN-gamma and TNF-alpha in IL-12 induced immunity, IFN-gamma and TNFR-p55 knockout mice were immunized with rSm14/IL-12 and no protection was achieved ... In an attempt to determine the role of IFN-gamma and TNF-alpha in IL-12 induced immunity, IFN-gamma and TNFR-p55 knockout mice were immunized with rSm14/IL-12 and no protection was achieved
Atkins et al., Br J Cancer 2003 (Carcinoma, Transitional Cell...) : By contrast, LPS- and CpG-oligonucleotides induced only IL-12 , indicating the potential to produce a Th1 response, which is likely to clear cancer most efficiently
Yang et al., Ann N Y Acad Sci 2003 (Diabetes Mellitus, Type 1) : Interruption of IL-12 mediated STAT4 activation prevented autoimmune diabetes in NOD mice
Xiong et al., J Biol Chem 2004 : As a consequence, the NO donor S-nitroso-N-acetylpenicillamine (SNAP) inhibits lipopolysaccharide (LPS) induced IL-12 p40 mRNA expression, protein production, and promoter activity in murine macrophages, dendritic cells, and the murine macrophage cell line RAW 264.7 ... As a consequence, the NO donor S-nitroso-N-acetylpenicillamine (SNAP) inhibits lipopolysaccharide (LPS) induced IL-12 p40 mRNA expression, protein production, and promoter activity in murine macrophages, dendritic cells, and the murine macrophage cell line RAW 264.7
Furbert-Harris et al., Cell Mol Biol Incl Cyto Enzymol 2003 (Neoplasm Metastasis...) : E-cadherin was up-regulated on DU 145 cells following co-culture with EosA and was induced on PC-3 by IL-10 and IL-12 , but not by EosA co-culture and 24 hr supematants
Athie-Morales et al., J Immunol 2004 : IL-12 and IFN-alpha both activate STAT4 , but with different kinetics ... The continuous presence of IL-12 was required for sustained STAT4 activation ... Furthermore, the cytokine IL-2 potentiated sustained IL-12/STAT4 responses through up-regulation of IL-12R expression and synergized with IL-12 in driving Th1 cell development
Ma et al., J Immunol 2004 : A role for c-Jun N-terminal kinase (JNK) MAPK in LPS induced IL-12p40 regulation in a promonocytic THP-1/CD14 cell line was demonstrated by using specific inhibitors of JNK activation, SP600125 and a dominant negative stress activated protein/extracellular signal regulated kinase kinase-1 mutant ... The role of AP-1 in IL-12p40 transcription was confirmed by using antisense c-fos and c-jun oligonucleotides ... A role for c-Jun N-terminal kinase (JNK) MAPK in LPS induced IL-12p40 regulation in a promonocytic THP-1/CD14 cell line was demonstrated by using specific inhibitors of JNK activation, SP600125 and a dominant negative stress activated protein/extracellular signal regulated kinase kinase-1 mutant ... Taken together, our results suggest that DXM may inhibit IL-12p40 production in LPS stimulated human monocytic cells by down regulating the activation of JNK MAPK , the AP-1, and NF-kappaB transcription factors ... Taken together, our results suggest that DXM may inhibit IL-12p40 production in LPS stimulated human monocytic cells by down regulating the activation of JNK MAPK, the AP-1 , and NF-kappaB transcription factors ... Taken together, our results suggest that DXM may inhibit IL-12p40 production in LPS stimulated human monocytic cells by down regulating the activation of JNK MAPK, the AP-1, and NF-kappaB transcription factors
Williams et al., J Immunol 2004 (Disease Susceptibility...) : Inhibition of PI3K resulted in increased serum levels of IL1-beta, IL-2, IL-6, IL-10, IL-12 , and TNF-alpha in septic mice
Subauste et al., J Infect Dis 2004 (AIDS-Related Opportunistic Infections...) : Recombinant CD154 partially restored secretion of IL-12 and IFN-gamma in response to T. gondii in cells from HIV infected patients
Wagner et al., J Immunol 2004 : Unlike IL-6, TNF-alpha, and IL-10, the Th1 cytokine IL-12p70 was detected only when both CpG ODN and CD40L were present, and its induction was independent of B cell receptor cross linking
Pekkari et al., Antioxid Redox Signal 2004 : Trx80 induces secretion of interleukin (IL)-12 in CD40+ monocytes from PBMC
Park et al., Int Immunol 2004 : IL-12 promotes T ( h ) 1 development/IFN-gamma expression by activating STAT4 ... IL-12 stimulation greatly enhanced the promoter binding activity of c-Jun/AP-1 , a critical transcription factor for IFN-gamma gene expression in wild-type T cells, but not in STAT4-deficient ( STAT4 ( -/- ) ) T cells ... IL-12 stimulation greatly enhanced the promoter binding activity of c-Jun/AP-1 , a critical transcription factor for IFN-gamma gene expression in wild-type T cells, but not in STAT4-deficient ( STAT4 ( -/- ) ) T cells
Morrison et al., J Immunol 2004 : Macrophage stimulating protein , the ligand for the stem cell derived tyrosine kinase/RON receptor tyrosine kinase, inhibits IL-12 production by primary peritoneal macrophages stimulated with IFN-gamma and lipopolysaccharide ... IL-12 , produced by APCs during the initial stages of an immune response, plays a pivotal role in the induction of IFN-gamma by NK and gammadeltaT cells and in driving the differentiation of Th1 cells, thus providing a critical link between innate and acquired immunity ... We also show that the ability of MSP to inhibit IL-12 production is independent of IL-10
Sugawara et al., Tohoku J Exp Med 2004 (Genetic Predisposition to Disease...) : Therefore, STAT1 regulates IL-12 expression and appears to be a critical transcription factor in controling mycobacterial infection ... Therefore, STAT1 regulates IL-12 expression and appears to be a critical transcription factor in controling mycobacterial infection
Satthaporn et al., Cancer Immunol Immunother 2004 (Breast Neoplasms) : PBDCs and LNDCs from patients expressed low levels of HLA-DR and CD86 , and induced decreased interleukin-12 (IL-12) secretion in vitro, compared with DCs from normal donors ( p < 0.01 )
Begum et al., Infect Immun 2004 : ( iii ) Interleukin-23 (IL-23), rather than IL-12p70 , was induced by BCG-CWS, while interferon-inducible genes were induced only by LPS
Tamura et al., Transplantation 2004 : beta2-AR agonists prevented the production of IFN-gamma and IL-12 in the presence of IL-18 but had no effect in the absence of IL-18
Laderach et al., Cell Immunol 2003 : 4-1BB-ligand is regulated on human dendritic cells and induces the production of IL-12
Hessle et al., Inflammation 2003 (Inflammation) : We have seen that Gram-positives preferentially induce IL-12 and TNF-alpha, whereas Gram-negatives induce more IL-10 , IL-6, and IL-8 ... We have seen that Gram-positives preferentially induce IL-12 and TNF-alpha, whereas Gram-negatives induce more IL-10, IL-6 , and IL-8 ... We have seen that Gram-positives preferentially induce IL-12 and TNF-alpha, whereas Gram-negatives induce more IL-10, IL-6, and IL-8
Cervi et al., J Immunol 2004 : Although DC exposed to both Ag undergo many of the maturation associated changes that accompany exposure to Pa alone, Pa-induced IL-12 production was inhibited by SEA
Zhou et al., J Immunol 2004 (Leukemia P388) : Macrophage LPS induced synthesis of IL-12 is inhibited following FcgammaR ligation ; TNF-alpha secretion is unchanged ... We report that microtubule associated serine/threonine kinase-205 kDa ( MAST205 ) is required for LPS induced IL-12 synthesis ... We report that microtubule associated serine/threonine kinase-205 kDa ( MAST205 ) is required for LPS induced IL-12 synthesis ... RNA interference mediated suppression of MAST205 results in the inhibition of LPS stimulated IL-12 promoter activity and IL-12 secretion, from both J774 cells and bone marrow derived macrophages ... Similarly, dominant negative MAST205 mutants inhibit LPS stimulated IL-12 synthesis and NF-kappaB activation, but do not affect IL-1 or TNF-alpha signaling ... Similarly, dominant negative MAST205 mutants inhibit LPS stimulated IL-12 synthesis and NF-kappaB activation, but do not affect IL-1 or TNF-alpha signaling
Schleicher et al., Eur J Immunol 2004 (Leishmaniasis, Cutaneous) : The IL-12 induced production of IFN-gamma by NK cells and CD8 ( + ) T cells was strongly suppressed in Tyk2 ( -/- ) mice at day 1 of infection, but partly regained during the late phase of infection ... Thus, Tyk2 is required for the IL-12 response of NK cells and CD8 ( + ) T cells in L. major infected mice, but not for the generation of Th1 cells and the ultimate control of the disease
Yamada et al., Eur J Immunol 2004 : We show here that C1q suppresses IL-12p40 production in LPS stimulated murine bone marrow derived dendritic cells ( BMDC ) ... Because neither globular head of C1q (gC1q) nor collagen-like region of C1q ( cC1q ) failed to suppress LPS induced IL-12p40 production, both gC1q and cC1q, and/or some specialized conformation of native C1q may be required for the inhibition
Luo et al., J Biol Chem 2004 : We now provide the first description of the human erythroid Kruppel-like factor (EKLF) in human primary macrophages and identify the role of EKLF in IL-12 p40 expression ... Functional analysis of the CACCC element revealed a dependent role for EKLF binding in activating IL-12 p40 transcription in resting RAW264.7 cells, whereas EKLF overexpression in the presence or absence of this element repressed IL-12 p40 transcription in interferon gamma/lipopolysaccharide stimulated RAW264.7 cells ... Murine endogenous IL-12 p40 mRNA was consistently induced by overexpressed EKLF in resting RAW264.7 cells, whereas EKLF suppressed IL-12 p40 expression in activated RAW264.7 cells
Virág et al., Med Sci Monit 2004 (Asthma...) : The production of TNF- alpha and IL-12 , but not IL-5 or IL-13, was also suppressed by PARP inhibition
Ross et al., Infect Immun 2004 : CyaA significantly augmented LPS induced IL-6 and IL-10 and inhibited LPS-driven tumor necrosis factor alpha and IL-12p70 production from bone marrow derived DC and macrophages ... CyaA significantly augmented LPS induced IL-6 and IL-10 and inhibited LPS-driven tumor necrosis factor alpha and IL-12p70 production from bone marrow derived DC and macrophages
Morimoto et al., Int Immunol 2004 (Drug-Induced Liver Injury...) : Second, OPN deficiency resulted in the reduction of IL-12 and IFN-gamma production at early stages
Aita et al., J Rheumatol 2004 (Arthritis, Rheumatoid) : Interleukin 12 (IL-12) and IL-18 synergistically induce interferon-gamma (IFN-gamma) production by T cell infiltrates in rheumatoid arthritis ( RA )
Hong et al., Int Immunopharmacol 2004 (Disease Models, Animal...) : In addition, treatment of murine macrophage ANA-1 cells with 2 mM CQ potently inhibited the release of TNF-alpha, IL-6 and IL-12 induced by CpG ODN and LPS
Kim et al., Pharmacol Res 2004 : SB203580 increased LPS induced expression of TNF-alpha and IL-12 ; PD98059 had no effect on these cytokines ... p38 signaling pathway is involved in LPS induced ROS, TNF-alpha and IL-12 production
Soumelis et al., Springer Semin Immunopathol 2004 (Hypersensitivity...) : TSLP activated human DCs produce Th2 attracting chemokines but no IL-12 , and induce naive CD4 ( + ) and CD8 ( + ) T cell differentiation into effector cells with a typical pro-allergic phenotype ... TSLP activated human DCs produce Th2 attracting chemokines but no IL-12 , and induce naive CD4 ( + ) and CD8 ( + ) T cell differentiation into effector cells with a typical pro-allergic phenotype
Boelen et al., Horm Metab Res 2004 (Starvation) : IL-12 is involved in regulation of IFNgamma and TNFalpha ... IL-12 is involved in regulation of IFNgamma and TNFalpha
Mason et al., J Immunol 2004 (Toxoplasmosis, Animal) : For example, c-Rel is essential for the production of IL-12 in response to LPS , but dispensable for IL-12 production in response to Toxoplasma Ag ... For example, c-Rel is essential for the production of IL-12 in response to LPS, but dispensable for IL-12 production in response to Toxoplasma Ag
Toyoda et al., J Immunol 2004 (Lymphoproliferative Disorders...) : Impairment of IL-12 dependent STAT4 nuclear translocation in a patient with recurrent Mycobacterium avium infection ... Using EMSA, confocal laser microscopy, and Western blotting, we demonstrated that the nuclear translocation of STAT4 in response to IL-12 is reduced in PHA activated T cells from the patient when compared with those from healthy subjects
Li et al., Mol Ther 2004 : Further functional analysis of Stat1 indicates that a lack of Stat1 expression inhibits the Il-12 mediated induction of IP10 , a known antiangiogenic gene
Mytar et al., Inflamm Res 2004 : In MDM, intracellular TNF alpha and IL-12 expression was induced by mannan, native and modified LDL , but not other ligands
Vicari et al., Novartis Found Symp 2004 (Neoplasms) : In particular, CpG plus anti-IL10R enhanced the TAA-specific immune response and triggered de novo IL-12 production
Pina et al., Infect Immun 2004 (Hypersensitivity, Delayed...) : In addition, IL-4 deficiency resulted in significantly reduced levels of pulmonary IL-12 , granulocyte-macrophage colony stimulating factor, IL-3, monocyte chemotactic protein 1, and specific antibody isotypes
Yang et al., J Autoimmun 2004 (Diabetes Mellitus, Type 1...) : STAT4 mediates IL-12 signaling and regulates T helper 1 ( Th1 ) cell differentiation
Pelloso et al., Clin Cancer Res 2004 (Lymphoma...) : In contrast to their striking defect in IL-12 induced IFN-gamma production, posttransplant patient PBMCs exhibit normal proliferative responses to IL-12 in vitro
Hagendorens et al., Cytokine 2004 (Asthma) : In contrast to isolated LPS stimulation, stimulation with LPS/IFN-gamma induced higher IL-12 production ; allergic asthmatic children showed a significantly lower IL-12 secretion after LPS/IFN-gamma stimulation as compared to healthy children ( 20 ( 5-247 ) vs 208 ( 7-775 ) pg/mL ; p=0.03 ) ... In contrast to isolated LPS stimulation, stimulation with LPS/IFN-gamma induced higher IL-12 production ; allergic asthmatic children showed a significantly lower IL-12 secretion after LPS/IFN-gamma stimulation as compared to healthy children ( 20 ( 5-247 ) vs 208 ( 7-775 ) pg/mL ; p=0.03 )
Kawakami et al., Microbes Infect 2004 (Tuberculosis, Pulmonary) : Interferon (IFN)-gamma plays an essential role in host defense against infection with Mycobacterium tuberculosis, and its synthesis is critically regulated by interleukin (IL)-12 , IL-18 and the recently identified IL-23
Gutzmer et al., Immunology 2004 : After up-regulation of the C3aR by IFN-alpha+ IFN-gamma, C3a significantly up-regulated the surface expression of CD54, CD83 and CD86, but not of CD40, CD80 or human leucocyte antigen ( HLA ) -DR. C3a had no effect on the production of interleukin (IL)-10 or IL-12p70 , or on the capacity of MoDC to stimulate autologous T-cell proliferation
Herskind et al., Strahlenther Onkol 2004 (Neoplasms...) : Preclinical strategies of the antitumor action of cytokines are presented and discussed based on the induction of IFN-gamma by IL-12
Sakai et al., J Reprod Immunol 2004 (Pre-Eclampsia) : These results suggest that elevated IL-18 secretion and decreased IL-12 secretion by PBMC may induce Th2 dominance in normal pregnancy, while elevated secretion of both IL-18 and IL-12 by PBMC may cause Th1 dominance in severe pre-eclampsia
Braedel et al., Br J Haematol 2004 : The release of IL-12 by BMDCs in response to A. fumigatus antigens was dependent on the expression of TLR2 , whereas the release of IL-6 was dependent on the expression of functional TLR4 molecules
El-Mezayen et al., Clin Immunol 2004 (Asthma...) : IFN-gamma production was detected in all cultures from nonallergic controls stimulated with IL-18 in the presence of IL-12 ; however, the results for five BA patients and five AD patients were under the detection limit for IFN-gamma ... Although the induction of IFN-gamma by IL-18 + IL-12 was impaired in around a quarter of the allergic patients, the impairment of the IFN-gamma production was completely restored by IL-2 in the presence of IL-18
Sigmundsdóttir et al., Clin Immunol 2004 : While adding IL-12 augmented the expression of CLA, superantigen induced expression of CD103 was markedly suppressed by IL-12 , which could be reversed by TGF-beta ... Thus, the expression of CLA and CD103 may be antagonistically regulated by IL-10 and IL-12 and the balance between these cytokines could influence the T cell migration of inflammatory cells into epithelial tissues
Schneider et al., J Immunol 2004 : IL-12 plus IL-18 rendered NK cells cytotoxic against Fas transfected target cells and promoted their production of IFN-gamma and TNF-alpha , which are both essential for sensitizing histamine producing cells to the Fas death pathway ... IL-12 plus IL-18 rendered NK cells cytotoxic against Fas transfected target cells and promoted their production of IFN-gamma and TNF-alpha, which are both essential for sensitizing histamine producing cells to the Fas death pathway
Conant et al., J Immunol 2004 (Disease Models, Animal...) : The activated T cells produced the proinflammatory cytokine, IFN-gamma in response to IL-12 , and IL-6 was secreted in response to CpG oligonucleotide
Litjens et al., J Invest Dermatol 2004 (Psoriasis) : No association was observed between the various IL12B promoter genotypes and the LPS stimulated production of IL-12p70 or IL-12p40 by blood cells
Mathur et al., Nat Med 2004 (MAP Kinase Signaling System) : Here we show that weak CD40 signals induce extracellular stress related kinase-1/2 ( ERK-1/2 ) -dependent IL-10 expression, whereas stronger signals induce p38 mitogen activated protein kinase ( p38MAPK ) -dependent IL-12 production ... Here we show that weak CD40 signals induce extracellular stress related kinase-1/2 ( ERK-1/2 ) -dependent IL-10 expression, whereas stronger signals induce p38 mitogen activated protein kinase ( p38MAPK ) -dependent IL-12 production
Rutella et al., Eur J Immunol 2004 : As shown by neutralization studies, IFN-alpha and, even more pronounced, IL-10 contained in post-G serum inhibited IL-12p70 release by post-G DC-like cells
Fedele et al., Eur J Immunol 2004 : CD38 is expressed on human mature monocyte derived dendritic cells and is functionally involved in CD83 expression and IL-12 induction ... CD38 is expressed on human mature monocyte derived dendritic cells and is functionally involved in CD83 expression and IL-12 induction ... Activation via agonistic anti-CD38 mAb induces up-regulation of CD83 expression and IL-12 secretion, whereas disruption of CD38/CD31 interaction inhibits CD83 expression, IL-12 secretion and MDDC induced allogeneic T cell proliferation ... Activation via agonistic anti-CD38 mAb induces up-regulation of CD83 expression and IL-12 secretion, whereas disruption of CD38/CD31 interaction inhibits CD83 expression, IL-12 secretion and MDDC induced allogeneic T cell proliferation
Wang et al., J Exp Med 2004 (Leishmaniasis, Cutaneous) : Although SLAM controls production of IL-12 , tumor necrosis factor, and nitric oxide in response to lipopolysaccharide (LPS) by macrophages, SLAM does not regulate phagocytosis and responses to peptidoglycan or CpG ... Although SLAM controls production of IL-12 , tumor necrosis factor, and nitric oxide in response to lipopolysaccharide (LPS) by macrophages, SLAM does not regulate phagocytosis and responses to peptidoglycan or CpG
Gumperz et al., J Leukoc Biol 2004 (Neoplasms) : Hence, CD1d restricted T cells may promote or prevent effective anti-tumor responses that are mediated by other lymphocytic effector cells by influencing IL-12 production by myeloid DCs and by their own production of early IFN-gamma in response to IL-12
Yu et al., J Immunol 2004 : CD40L induced monocyte derived DC IL-12 production was mediated by both the p38 MAPK and PI3K pathways ... CD40L induced monocyte derived DC IL-12 production was mediated by both the p38 MAPK and PI3K pathways
Feng et al., Int Immunopharmacol 2004 : When macrophages were primed with IFN-gamma, resveratrol suppressed the expression of HLA-ABC, HLA-DR, CD80, CD86 and inhibited production of TNF-alpha, IL-12 , IL-6 and IL-1beta induced by LPS
van Rietschoten et al., Tissue Antigens 2004 (Asthma) : Interleukin-12 (IL-12) is a potent inducer of interferon-gamma production by T cells and is a major factor for the development of T-helper 1 ( Th1 ) cells
Takahashi et al., J Neuroimmunol 2004 : While endogenous IL-18 produced by salbutamol and terbutaline reached a sufficient concentration to induce IL-12 production, these beta 2-AR agonists did not induce the production of IL-12 at all
Siwkowski et al., Nucleic Acids Res 2004 : CD40 dependent induction of IL-12 in primary murine macrophages was attenuated in cells treated with ISIS 208529
Itoi et al., J Interferon Cytokine Res 2004 (Acute Disease...) : However, IL-18Ralpha expression did not increase on the CD4+ T cells of an IL-12-deficient host, indicating the IL-12 dependent upregulation of IL-18Ralpha expression on CD4+ T cells during aGVHD
Sague et al., J Interferon Cytokine Res 2004 (Toxoplasmosis) : These studies revealed that whereas stimulation of resting NK cells with interleukin-12 (IL-12) or IL-18 caused increased expression of CD44 , only IL-2 or IL-15 led to the upregulation and activation of CD44
Lund et al., J Immunol 2004 : IL-12 signaling through STAT4 is essential for induction of optimal levels of IFN-gamma production and commitment of Th1 cells ... In addition to IL-12 signaling mediated by STAT4 , STAT4 independent induction of a number of genes was observed immediately in response to Th1 induction
Del Rio et al., J Immunol 2004 : Although we identify TLR2 as the receptor triggering CCL2 production, parasite induced IL-12 release did not involve this TLR ... The production of both IL-12 and CCL2 was increased after neutrophil activation with IFN-gamma ... However, the synergistic effect of IFN-gamma on IL-12 , but not CCL2, was dependent upon Stat1 signal transduction
Papadakis et al., J Immunol 2004 : In the current study we show that TL1A or an agonistic anti-DR3 mAb synergize with IL-12/IL-18 to augment IFN-gamma production in human peripheral blood T cells and NK cells
Sharma et al., Immunol Lett 2004 : It is observed that rLcrV and rYopB inhibited the LPS induced expression of TNF-alpha, IFN-gamma, KC, IP-10, and IL-12 in macrophages
Raymond et al., Vaccine 2004 : Addition of cytokines to mDCs treated with Mtb altered T-cell cytokine mRNA expression such that TNF-alpha, IFN-gamma or IL-12 increased IFN-gamma ; IL-12 and IFN-gamma suppressed IL-10 , while IL-10 and IL-12 enhanced IL-13 ... Addition of cytokines to mDCs treated with Mtb altered T-cell cytokine mRNA expression such that TNF-alpha, IFN-gamma or IL-12 increased IFN-gamma ; IL-12 and IFN-gamma suppressed IL-10, while IL-10 and IL-12 enhanced IL-13
Tada et al., Cytokine 2004 : Differential effects of LPS and TGF-beta on the production of IL-6 and IL-12 by Langerhans cells, splenic dendritic cells, and macrophages ... We examined modulatory effects of lipopolysaccharide (LPS) on IL-6 and IL-12 production by mouse Langerhans cells ( LC ), spleen derived CD11c+ dendritic cells ( DC ), and macrophages ( Mphi ) ... Low dose LPS ( 1 ng/ml ) increased IL-6 and IL-12 p40 production by Mphi ... In contrast, only high dose LPS ( 1 microg/ml ) induced IL-6 but not IL-12 p40 production by LC
Yang et al., Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2003 : [ Study on a new IL-12 inducing gene NAP2 ] ... NAP2 could activate NF-kappaB and IL-12 promoter ... In addition, NAP2 could induce IL-12 expression in macrophage cell line J774 ... In addition, NAP2 could induce IL-12 expression in macrophage cell line J774 ... NAP2 as a newly cloned NF-kappaB activating gene can activate IL-12 promoter and induce IL-12 expression in macrophages
Strengell et al., J Leukoc Biol 2004 : We demonstrate here that in addition to activating IFN-gamma gene expression, IFN-alpha/beta and IL-12 enhance the mRNA expression of IL-21 in activated human T cells
Eriksson et al., J Leukoc Biol 2004 : Stimulation of uNK cells with interleukin (IL)-12 and IL-15, both of which are expressed in the human EM, induced interferon-gamma (IFN-gamma) and IL-10 production ... Stimulation of uNK cells with interleukin (IL)-12 and IL-15, both of which are expressed in the human EM, induced interferon-gamma (IFN-gamma) and IL-10 production
Matsumoto et al., J Immunol 2004 (Inflammation) : We report in this work that, unexpectedly, simvastatin enhances LPS induced IL-12p40 production by murine macrophages, and that it does so by activating the IL-12p40 promoter ... This occurs via a c-Fos- and c-Jun based mechanism ; we demonstrate that ectopic expression of c-Jun activates the IL-12p40 promoter, whereas expression of c-Fos inhibits IL-12p40 promoter activity ... Simvastatin prevents LPS induced c-Fos expression, thereby relieving the inhibitory effect of c-Fos on the IL-12p40 promoter ... Concomitantly, simvastatin induces the phosphorylation of c-Jun by the c-Jun N-terminal kinase, resulting in c-Jun dependent activation of the IL-12p40 promoter ... The fact that simvastatin potently augments LPS induced IL-12p40 and TNF-alpha production has implications for the treatment of bacterial infections in statin treated patients
Hubert et al., J Immunol 2004 : CpG ODN and CD40 ligand , but not influenza virus, induced IL-12p40 and IL-6 secretion
Mattner et al., J Immunol 2004 (Disease Progression...) : IFN-beta treatment of BALB/c mice restored the NK cell cytotoxic activity, increased the lymphocyte proliferation, and augmented the production of IFN-gamma and IL-12 in the draining lymph node
Kawashima et al., Arthritis Rheum 2004 (Arthritis, Rheumatoid) : The production of IL-18BP in response to IL-12 and IL-18 was regulated differently in blood and synovial cells
Gentile et al., Ann Allergy Asthma Immunol 2004 (Asthma...) : Median LPS induced IL-12 levels were similar in all groups
Tazaki et al., Cell Immunol 2004 (Asthma) : Significant increases in the expression of IL-12R beta2 chain before and after stimulation of CD4 ( + ) T cells with IL-12 or IFN-gamma were observed in RIT treated patients compared with that in control patients ... Allergen RIT increases IL-12 production and IL-12R beta2 chain expression and thus may convert cytokine production from Th2 to Th1 or Th0 type in allergic asthma
Ruth et al., J Immunol 2004 (Glomerulonephritis...) : CD40-CD154 interactions are critical for IL-12 production and Th1 polarization of immune responses ... CD40-CD154 interactions are critical for IL-12 production and Th1 polarization of immune responses ... Thus, CD40 directed IL-12 drives Th1 generation and effector cell recruitment but CD40 is required for activation ... This study demonstrated that CD40 induced IL-12 is required for generation of systemic Th1 immunity to nephritogenic Ags, and that IL-12 enhances Th1 effector cell recruitment to peripheral sites of Ag presentation via generation of local chemokines
Odyniec et al., J Immunol 2004 (Encephalomyelitis, Autoimmune, Experimental) : That gammadelta T cells enhanced the MBP response and severity of adoptive EAE by stimulating IL-12 production was supported by experiments showing that reconstitution of the gammadelta T cell population restored IL-12 production, and that gammadelta T cell depletion induced effects were reversed by the addition of IL-12
Ohta et al., Am J Reprod Immunol 2004 (Ovarian Neoplasms) : The PBMC collected from both cancer bearing patients and healthy subjects showed IL-2- and/or IL-12 induced IFN-gamma production
Sivori et al., Proc Natl Acad Sci U S A 2004 : In the presence of IL-12 , freshly isolated NK cells responded to double stranded RNA or unmethylated CpG DNA and expressed CD69 and CD25 activation markers ... In the presence of IL-12 , freshly isolated NK cells responded to double stranded RNA or unmethylated CpG DNA and expressed CD69 and CD25 activation markers
Stoffels et al., J Autoimmun 2004 (Necrosis) : In contrast, NOD macrophages failed to up-regulate IL1beta and IL12p40 in response to IFNgamma
Yu et al., J Immunol 2004 : We further show that STAT1 signals play major roles in inducing SOCS expression in Th cells and that induction of SOCS expression by IL-4, IL-12 , or IFN-gamma is compromised in STAT1-deficient primary Th cells
Liu et al., J Immunol 2004 (Genetic Predisposition to Disease...) : Our recent study has revealed an intimate relationship between IRF-1 and IL-12 in that IRF-1 regulates the production of IL-12 by selectively controlling transcriptional activation of IL-12 p35 gene
McKee et al., J Immunol 2004 (Schistosomiasis mansoni) : Mice infected with Schistosoma mansoni develop polarized Th2 responses in which Th1 responses are prevented by IL-10 mediated suppression of IL-12 production
Borg et al., Blood 2004 : Knock-out, small interfering RNA or neutralizing antibodies targeting interleukin 12 (IL-12) subunits revealed a critical role for IL-12 in NK cell interferon gamma (IFN-gamma) secretion promoted by mDCs
Al-Darmaki et al., Clin Diagn Lab Immunol 2004 (Periodontal Diseases) : In addition, PAF induced monocyte derived dendritic cells ( DC ) but not macrophages ( MPhi ) to secrete IL-12 and IL-18
Hoentjen et al., Blood 2005 : In this study, we investigated the role of nuclear factor-kappa B (NF-kappaB) and signal transducers and activators of transcription 3 ( STAT3 ) in lipopolysaccharide (LPS) induced IL-12p40 gene expression in bone marrow derived-dendritic cells ( BMDCs ) isolated from wild-type ( WT ) and IL-10 ( -/- ) mice ... In this study, we investigated the role of nuclear factor-kappa B (NF-kappaB) and signal transducers and activators of transcription 3 ( STAT3 ) in lipopolysaccharide (LPS) induced IL-12p40 gene expression in bone marrow derived-dendritic cells ( BMDCs ) isolated from wild-type ( WT ) and IL-10 ( -/- ) mice ... Chromatin immunoprecipitation demonstrated enhanced NF-kappaB ( cRel, RelA ) binding to the IL-12p40 promoter in IL-10 ( -/- ) but not WT BMDCs. Interestingly, LPS induced STAT3 phosphorylation in WT but not IL-10 ( -/- ) BMDCs, a process blocked by IL-10 receptor blocking antibody ... Chromatin immunoprecipitation demonstrated enhanced NF-kappaB ( cRel, RelA ) binding to the IL-12p40 promoter in IL-10 ( -/- ) but not WT BMDCs. Interestingly, LPS induced STAT3 phosphorylation in WT but not IL-10 ( -/- ) BMDCs, a process blocked by IL-10 receptor blocking antibody ... Chromatin immunoprecipitation demonstrated enhanced NF-kappaB ( cRel, RelA ) binding to the IL-12p40 promoter in IL-10 ( -/- ) but not WT BMDCs. Interestingly, LPS induced STAT3 phosphorylation in WT but not IL-10 ( -/- ) BMDCs, a process blocked by IL-10 receptor blocking antibody ... Adenoviral gene delivery of a constitutively active STAT3 but not control green fluorescence protein ( GFP ) virus blocked LPS induced IL-12p40 gene expression and cRel recruitment to the IL-12p40 promoter ... In conclusion, dysregulated LPS induced IL-12p40 gene expression in IL-10 ( -/- ) mice is due to enhanced NF-kappaB recruitment to the IL-12p40 promoter in the absence of activated STAT3
Newton et al., J Leukoc Biol 2004 (Legionnaires' Disease) : The results showed that RU486 did not attenuate the THC induced suppression of serum interleukin (IL)-12 or interferon-gamma (IFN-gamma) ... Although L. pneumophila infection increased urinary PGE2, THC pretreatment did not enhance this response ; in addition, treatment with the cyclooxygenase inhibitor, indomethacin, did not block the THC induced suppression of IL-12 and IFN-gamma
Elenkov et al., Ann N Y Acad Sci 2004 (Immune System Diseases...) : Evidence accumulated over the last 5-10 years indicates that glucocorticoids (GCs) inhibit the production of interleukin (IL)-12 , interferon (IFN)-gamma, IFN-alpha, and tumor necrosis factor (TNF)-alpha by antigen presenting cells (APCs) and T helper ( Th ) 1 cells, but upregulate the production of IL-4, IL-10, and IL-13 by Th2 cells
Teleshova et al., J Immunol 2004 : IL-12 production and CD80/CD86 expression by MDC/PDC mixtures was further enhanced by CD40L and ISS-ODN treatment
Wassink et al., J Immunol 2004 : ICOS expression by activated human Th cells is enhanced by IL-12 and IL-23 : increased ICOS expression enhances the effector function of both Th1 and Th2 cells ... In this study, we show that ICOS expression on human Th cells is not increased by IL-4, but by IL-12 and by IL-23 instead ... As the ICOS enhancing potentials of IL-12 and IL-23 were maintained for several days after stimulation, both on Th1 and Th2 cells, we propose the concept that local regulation of ICOS expression on activated Th cells by IL-12 and/or IL-23 may provide a powerful means to amplify effector T cell responses in peripheral tissues, independently of the polarized state of the Th cells
Skinner et al., J Immunol 2004 (Bordetella Infections...) : The B. bronchiseptica type III secretion system ( TTSS ) mediated the increase in MHCII, CD86, and CD80 surface expression, while the inhibition of CD40 and IL-12 expression was mediated by adenylate cyclase toxin ( ACT )
Rogers et al., J Parasitol 2004 : We found that the principal cytokine produced was IFN-gamma and that its production was regulated by IL-10 and IL-12
Chambers et al., Clin Exp Immunol 2004 (HIV Infections) : HIV infection of THP-1 myeloid cells resulted in decreased LPS induced nuclear factor binding to the NF-kappaB, AP-1, and Sp1 sites of the IL-12 p40 promoter
Xu et al., World J Gastroenterol 2004 : GM-CSF propagated DCs, GM-CSF+NF-kappaB decoy ODNs or scrambled ODNs propagated DCs were treated with LPS for 18 h to determine whether NF-kappaB decoy ODNs could prevent LPS induced IL-12 production in DCs ... NF-kappaB decoy ODNs completely abrogated IL-4 induced DC maturation and allocostimulatory activity as well as LPS induced NF-kappaB activation and IL-12 protein expression in DCs ... NF-kappaB decoy ODNs completely abrogated IL-4 induced DC maturation and allocostimulatory activity as well as LPS induced NF-kappaB activation and IL-12 protein expression in DCs
Kawashima et al., Ann Rheum Dis 2005 (Arthritis, Rheumatoid) : Reduction of systemic inflammation with infliximab treatment increased IFN gamma production in response to IL12 or IL12+IL18
Yang et al., J Immunol 2004 : However, HPV16 VLP stimulated IL-12 production by CD8alpha ( + ) CD11c ( + ) DCs is augmented by autocrine IFN-gamma signaling ... However, HPV16 VLP stimulated IL-12 production by CD8alpha ( + ) CD11c ( + ) DCs is augmented by autocrine IFN-gamma signaling
Berenson et al., Eur J Immunol 2004 : We previously reported that IL-12 , but not IFN-alphaA/D, induces T helper type ( Th ) 1 development and STAT4 phosphorylation in murine CD4+ T cells
Byrne et al., Eur J Immunol 2004 (Whooping Cough) : These findings suggest that NK cells confer resistance to B. pertussis by activating IL-12 mediated production of IFN-gamma , which enhances the anti-bacterial activity of macrophages, but also promotes the differentiation of Th1 cells
Friebe et al., J Virol 2004 : Both IL-18 and IL-12 are involved in PPVO mediated IFN-gamma release by T cells and/or NK cells
French et al., Blood 2005 (Sezary Syndrome) : This defect may explain the poor IL-12 production, because IL-12 production by anti-CD3 stimulated PBMCs was dependent on CD40L in healthy donors ... Importantly however, the inability of SzS PBMCs to appropriately produce IL-12 and TNF-alpha could be restored by recombinant hexameric CD40L
Józefowski et al., J Leukoc Biol 2004 : Ligation of SR-A also inhibited lipopolysaccharide plus interferon-gamma stimulated interleukin-12 (IL-12) release, by 25 % in AMs and by 68 % in thioglycollate elicited PMs, consistent with different levels of SR-A expression
Harada et al., J Interferon Cytokine Res 2004 : The addition of recombinant murine GM-CSF ( rMuGM-CSF ) to spleen cell cultures from various strains of mice synergistically enhanced IFN-gamma, TNF-alpha and IL-12p70 in the presence of SCG ... In contrast, neutralizing GM-CSF using anti-GM-CSF monoclonal antibody ( mAb ) significantly inhibited IFN-gamma, TNF-alpha, and IL-12p70 elicited by SCG
Manca et al., Infect Immun 2004 (Tuberculosis, Pulmonary) : In contrast, CDC1551 induced more IL-12 and other molecules associated with phagocyte activation and Th1 protective immunity
Chen et al., J Immunol 2004 : PS liposomes inhibited the up-regulation of HLA-ABC, HLA-DR, CD80, CD86, CD40, and CD83, as well as the production of IL-12p70 by human DCs in response to LPS ... Exogenous IL-12 restored IFN-gamma production by CD4 ( + ) T cells
Cameron et al., J Immunol 2004 (Leishmaniasis) : Infection with lesion derived Leishmania mexicana amastigotes inhibited LPS induced IL-12 production by mouse bone marrow derived macrophages
Baj-Krzyworzeka et al., Cancer immunity 2004 (Colorectal Neoplasms...) : Treatment of monocytes following coculture with IFN-gamma, TNF-alpha, GM-CSF, PPD or IFN-gamma in combination with these agents reversed the depressed TNF-alpha release, whereas IL-12 production was enhanced by IFN-gamma alone
Lin et al., Acta Paediatr Taiwan 2004 (Nephrosis, Lipoid) : The results showed sera IL-12 levels and LPS stimulated IL-12 productions for PBMNCs were significantly increased as compared with those of normal controls and in remission stage
Ichikawa et al., Biol Pharm Bull 2004 : These results suggest that gallate containing catechins, particularly EGCG, inhibits LPS induced IL-12p40 production in murine macrophages by inhibiting p38 MAPK while enhancing p44/p42 ERK, leading to the inhibition of IkappaBalpha degradation and NF-kappaB activation ... These results suggest that gallate containing catechins, particularly EGCG, inhibits LPS induced IL-12p40 production in murine macrophages by inhibiting p38 MAPK while enhancing p44/p42 ERK, leading to the inhibition of IkappaBalpha degradation and NF-kappaB activation
Mavropoulos et al., Blood 2005 : IL-12 and IL-18 rapidly and synergistically induced the secretion of IFN-gamma by freshly purified human peripheral blood lymphocytes ... Mitogen activated protein kinase p38 was activated strongly by IL-18 and weakly by IL-12 in natural killer cells but was not activated by either cytokine in T cells ... Therefore, the synergistic induction of IFN-gamma by IL-12 and IL-18 in natural killer cells is mediated at least in part by p38 dependent and 3 ' UTR mediated stabilization of IFN-gamma mRNA
Musikacharoen et al., Blood 2005 : Interleukin-15 induces IL-12 receptor beta1 gene expression through PU.1 and IRF 3 by targeting chromatin remodeling ... Additionally, IL12RB1 promoter activation by IFN-gamma was enhanced by the coexpression of a coactivator protein, CBP
Fagerås Böttcher et al., J Allergy Clin Immunol 2004 (Asthma...) : LPS induced IL-12 ( p70 ), IL-10, and IFN-gamma responses of PBMCs from 69 of the children were analyzed by means of ELISA ... Decreased LPS induced IL-12 ( p70 ) and IL-10 responses were associated with the TLR4 ( Asp299Gly ) polymorphism and independently with asthma, especially atopic asthma
Muthian et al., J Clin Immunol 2004 (Disease Models, Animal...) : In vitro treatment of activated T cells with quercetin blocks IL-12 induced tyrosine phosphorylation of JAK2, TYK2, STAT3 , and STAT4, resulting in a decrease in IL-12 induced T cell proliferation and Th1 differentiation ... In vitro treatment of activated T cells with quercetin blocks IL-12 induced tyrosine phosphorylation of JAK2, TYK2, STAT3 , and STAT4, resulting in a decrease in IL-12 induced T cell proliferation and Th1 differentiation ... In vitro treatment of activated T cells with quercetin blocks IL-12 induced tyrosine phosphorylation of JAK2, TYK2, STAT3, and STAT4 , resulting in a decrease in IL-12 induced T cell proliferation and Th1 differentiation ... In vitro treatment of activated T cells with quercetin blocks IL-12 induced tyrosine phosphorylation of JAK2, TYK2 , STAT3, and STAT4, resulting in a decrease in IL-12 induced T cell proliferation and Th1 differentiation ... In vitro treatment of activated T cells with quercetin blocks IL-12 induced tyrosine phosphorylation of JAK2 , TYK2, STAT3, and STAT4, resulting in a decrease in IL-12 induced T cell proliferation and Th1 differentiation
Bereta et al., Cancer Gene Ther 2004 : However, replication-competent rV-CD40L did not stimulate IL-12 under similar conditions ... We observed a high level of CD40L protein on purified viral particles and demonstrated that induction of IL-12 by nonreplicating rV-CD40L was blocked by anti-CD40 antibodies suggesting that functional CD40L on viral particles contributed to alterations in IL-12 synthesis
Aliberti et al., Immunol Rev 2004 (Toxoplasmosis) : Evidence accumulated in recent years suggests that dendritic cells (DCs) play a major role in the initiation of IL-12-driven host resistance and that IL-12 synthesis by DCs is carefully regulated to avoid overproduction
Cross et al., FEMS Immunol Med Microbiol 2004 : All bacteria induced marked secretion of IL-12 and TNFalpha by cells, while only coliforms induced production of IL-10 ; there was minimal or no induction of IL-18 or TGFbeta
Abe et al., Hepatology 2004 (Disease Susceptibility...) : In conclusion, increased IL-10 and reciprocally suppressed IL-12 production by Kupffer cells are responsible for deteriorated resistance to bacterial infection in BDL mice
Pasquier et al., J Leukoc Biol 2004 : Cross linking of FcalphaRI but not of TfR resulted in up-regulation of major histocompatibility complex ( MHC) class II/CD86 expression and secretion of IL-10 and IL-12 by immature Mo-DC
Schleicher et al., Blood 2005 : Notably, IL-12/IL-18 still induced IFN-gamma mRNA in highly purified macrophages from wild-type mice and in macrophages from RAG2 ( -/- ) gamma-chain ( -/- ) mice, whereas nuclear translocation of signal transducer and activator of transcription 4 ( STAT4 ) and production of IFN-gamma protein were no longer detectable
Giambartolomei et al., J Immunol 2004 (Inflammation) : Lipidated ( L ) -Omp16 and L-Omp19 , but not their unlipidated forms, induced the secretion of TNF-alpha, IL-6, IL-10, and IL-12 in a time- and dose dependent fashion
Mason et al., Infect Immun 2004 : Recent studies suggest that components of the TLR pathway are required for the production of IL-12 in response to the parasite Toxoplasma gondii ; however, the production of IL-12 in response to this parasite is independent of NF-kappaB activation ... To elucidate the intracellular signaling pathways involved in the production of IL-12 in response to soluble toxoplasma antigen ( STAg ) , wild-type and TRAF6 ( -/- ) mice were inoculated with STAg, and the production of IL-12 ( p40 ) was determined ... TRAF6 ( -/- ) mice failed to produce IL-12 ( p40 ) in response to STAg , and TRAF6 ( -/- ) macrophages stimulated with STAg also failed to produce IL-12 ( p40 ) ... The studies presented here demonstrate for the first time that the production of IL-12 ( p40 ) in response to toxoplasma is dependent upon TRAF6 and p38 MAPK ... The studies presented here demonstrate for the first time that the production of IL-12 ( p40 ) in response to toxoplasma is dependent upon TRAF6 and p38 MAPK
Balic et al., Eur J Immunol 2004 (Strongylida Infections) : LPS induced high IL-12p70 levels, except in DC that had been pre incubated with NES
Shirota et al., J Immunol 2004 (Autoimmune Diseases) : Findings indicate that these ODN inhibit IFN-gamma induced STAT1 phosphorylation and IL-12 induced STAT3 and STAT4 phosphorylation ... Findings indicate that these ODN inhibit IFN-gamma induced STAT1 phosphorylation and IL-12 induced STAT3 and STAT4 phosphorylation
Nasu et al., Fertil Steril 2004 : The effects of interleukin (IL)-2, IL-4, IL-5, IL-10, IL-12 , IL-13, interferon-gamma (IFN-gamma), and tumor necrosis factor-beta (TNF-beta) on the production of TARC and MDC were investigated
Hart et al., Gut 2004 : VSL # 3 diminished proinflammatory effects of LPS by decreasing LPS induced production of IL-12 while maintaining IL-10 production
Ghosh et al., Immunobiology 2004 : Some species of Leishmania enhance the surface expression of co-stimulatory molecules and CD40-ligand induced IL-12 production in DC
Koh et al., J Korean Med Sci 2004 (Asthma...) : BCG treated DCs enhanced significantly the expressions of CD80, CD86, and CD40, and the productions of IL-12 and IL-10
Watanabe et al., Shock 2004 (Acute-Phase Reaction...) : The augmented IL-12 production by C57BL/6 macrophages increased interferon-gamma and, in contrast, decreased IL-13 production by CD4+ T cells
Ebert et al., Clin Exp Immunol 2004 : Furthermore, IL-12 augmented IL-15 stimulated release of serine esterases as well as expression of perforin and FL by IELs, but not TNF-alpha
Rossi et al., Int Immunol 2004 : Nevertheless, LPS potently induced DCs to produce TNF and IL-12 , but SAg did not, even though they efficiently activated DCs ... Specifically, pre priming with SAg enhanced LPS mediated DC synthesis of TNF and IL-12
Ma et al., Cell Biol Int 2004 : IL-23 p19 and IL-12 p35 mRNAs were expressed in fresh PBMC, and expression of all IL-23 and IL-12 subunits was up-regulated by LPS and SAC
Liebau et al., Anticancer Res 2004 (Bone Neoplasms...) : In contrast to IL-18, IL-23 showed a moderate increase of IFN-gamma induction when transfected alone and could only slightly increase the IFN-gamma induction mediated by IL-12 ... IL-18 enhanced IFN-gamma induction when applied alone and was able to increase the IFN-gamma production that was induced by IL-12
Kusaba et al., J Biol Chem 2005 : Interleukin (IL)-12 mediated interferon (IFN)-gamma production by activated T cells has been shown to be CsA-insensitive ... IL-12 induced phosphorylation of Stat3 on Ser-727 was affected by rapamycin, which may be due to the effect of rapamycin on the IL-12 induced interaction between mammalian target of rapamycin (mTOR) and Stat3 ... IL-12 induced phosphorylation of Stat3 on Ser-727 was affected by rapamycin, which may be due to the effect of rapamycin on the IL-12 induced interaction between mammalian target of rapamycin (mTOR) and Stat3 ... These results suggest that mTOR may play a major role in IL-12 induced IFN-gamma production by activated T cells
Chang et al., Mol Cells 2004 (Inflammation...) : Activation of NF-kappaB leads to the production of proinflammatory cytokines such as IL-12 and TNF-alpha that are involved in innate and adaptive immunity ... We also observed that the parasite inhibited the nuclear translocation of NF-kappaB at 8 h, and diminished IL-12 and TNF-alpha production in response to LPS
Berenson et al., Immunol Rev 2004 : Another unresolved issue is the role of type I IFNs in substituting for IL-12 mediated activation of signal transducer and activator of transcription 4 ( Stat4 ) and induction of IFN-gamma in either murine or human T cells
Chen et al., Sarcoidosis Vasc Diffuse Lung Dis 2004 (Alveolitis, Extrinsic Allergic) : The concentration of the LPS stimulated IL-12 production positively correlated with the percentage of lymphocytes ( r = 0.72, p = 0.011 ), and negatively correlated with the percentage of macrophages ( r = -0.88, p < 0.001 )
Reich et al., Exp Dermatol 2004 (Dermatitis, Atopic...) : Maturation, as determined by up-regulation of CD83 and CD86 surface expression, and production of IL-16, but not production of IL-10 and IL-12p40 was impaired in day 8 DCs derived from AD patients compared to those from healthy donors
Piskin et al., Exp Dermatol 2004 (Psoriasis) : The expression of the type-1 cytokine interferon-gamma (IFN-gamma) is regulated by interleukin-12 (IL-12) , IL-15, IL-18 and IL-23 ; however, not much is known about the effect of this therapy on the levels of these cytokines in lesional psoriatic skin in situ ... Thus, we concluded that the decrease of IFN-gamma expression in psoriasis lesions after NB-UVB therapy could be a result of diminished expression of IL-12 , IL-18 and IL-23 in lesional skin
Hailu et al., Am J Trop Med Hyg 2004 (Leishmaniasis, Visceral) : Production of IFN-gamma is regulated by interleukin (IL)-12 , IL-18, and IL-15
Fairweather et al., Am J Pathol 2004 (Cardiomyopathy, Dilated...) : Th1-type immune responses, mediated by interleukin (IL)-12 induced interferon (IFN)-gamma , are believed to exacerbate autoimmune diseases including myocarditis
Kabashima et al., FEBS Lett 2004 (Hypersensitivity, Delayed...) : Here, we found that our recently synthesized NF-kappaB inhibitor attenuated an ovalbumin-specific delayed-type hypersensitivity response in vivo and suppressed production of IL-12 by dendritic cells and TH1 cytokines by T cells in vitro
Li et al., Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 2004 (Malaria, Falciparum) : GM-CSF , IL-4 and IL-12 encoding plasmids all promoted mice immune response to VR1020/E, the antibody level increased 7 to 10 times and splenocyte proliferation was enhanced too
Daly et al., Vaccine 2005 : Plasmid DNA stimulated IL-10 and IL-12 production by macrophages and dendritic cells (DCs) in vitro and anti-IL-10 antibodies enhanced IL-12 production and DC maturation in response to gp120pDNA
Zhu et al., J Biol Chem 2005 (Inflammation) : The ERK signaling pathway is not involved in TNF-alpha and nitric oxide production, but, interestingly, negatively regulates the expression of IL-6 and IL-12 ... Furthermore, p38 is critical for the production of IL-6 and IL-12 but is only marginally required for the production of TNF-alpha and nitric oxide
Fairweather et al., J Immunol 2005 (Coxsackievirus Infections...) : Th1-type immune responses, mediated by IL-12 induced IFN-gamma , are believed to exacerbate certain autoimmune diseases
Persky et al., J Immunol 2005 : IL-12 , but not IFN-alpha, promotes STAT4 activation and Th1 development in murine CD4+ T cells expressing a chimeric murine/human Stat2 gene ... Furthermore, IL-12 , but not IFN-alpha, promoted acute IFN-gamma secretion in collaboration with IL-18 stimulation in both CD4 ( + ) and CD8 ( + ) T cells
Tchórzewski et al., Med Sci Monit 2005 (Autoimmune Diseases...) : We analyzed reactive oxygen intermediate ( ROI ) production by peripheral blood granulocytes, the production of IL-2, IL-5, IL-10, IL-12 , and TNF-alpha cytokines by lymphocytes, and the activation of CD25, CD26, CD69, CD71 , and HLA DR antigen expression ... We analyzed reactive oxygen intermediate ( ROI ) production by peripheral blood granulocytes, the production of IL-2, IL-5, IL-10, IL-12 , and TNF-alpha cytokines by lymphocytes, and the activation of CD25, CD26, CD69 , CD71, and HLA DR antigen expression ... We analyzed reactive oxygen intermediate ( ROI ) production by peripheral blood granulocytes, the production of IL-2, IL-5, IL-10, IL-12 , and TNF-alpha cytokines by lymphocytes, and the activation of CD25, CD26 , CD69, CD71, and HLA DR antigen expression ... We analyzed reactive oxygen intermediate ( ROI ) production by peripheral blood granulocytes, the production of IL-2, IL-5, IL-10, IL-12 , and TNF-alpha cytokines by lymphocytes, and the activation of CD25 , CD26, CD69, CD71, and HLA DR antigen expression
Akamine et al., Infect Immun 2005 : Treatment of L. pneumophila sonicated lysate with proteinase K and heating did not abolish TLR2 dependent IL-12p40 production
Sato et al., Eur J Immunol 2005 (Shwartzman Phenomenon) : The neutralization of either IL-12 induced IFN-gamma or LPS induced TNF-alpha improved the survival of middle aged ( 25-week-old ) mice ... The neutralization of either IL-12 induced IFN-gamma or LPS induced TNF-alpha improved the survival of middle aged ( 25-week-old ) mice
Chelvarajan et al., J Leukoc Biol 2005 (Inflammation) : Upon stimulation with LPS , in comparison with young MPhi, aged MPhi secreted reduced amounts of IL-6, tumor necrosis factor alpha, IL-1beta, and IL-12 , cytokines necessary for B cells to respond to TI Ag
Sun et al., J Immunol 2005 : Polarized Th1 responses are induced by dendritic cells (DCs) , which respond to pathogen derived TLR ligands to produce IL-12 and related cytokines that are instrumental in Th1 cell outgrowth, and coordinately process and present Ag in the context of MHC class II to activate naive Th cells
Kamath et al., J Immunol 2005 : In vitro, IFN-alphabeta, IL-18, and IL-12 all contributed to DC stimulation of NK cell IFN-gamma , whereas IFN-alphabeta was shown to be important for induction of T cell bystander activation and NK cell IFN-gamma production in vivo
Yu et al., J Immunol 2005 : Interestingly, IL-12 induced activation of Jak2 and STAT4 , critical components for IL-12 mediated cellular responses, was shortened or attenuated in mutant T cells ... Interestingly, IL-12 induced activation of Jak2 and STAT4, critical components for IL-12 mediated cellular responses, was shortened or attenuated in mutant T cells
Moreira et al., Immunobiology 2004 : Synergistic activation by IL-12/IL-18 induced the expression of the IFNgamma gene in Mphi from both mouse strains
Kozłowska et al., Neoplasma 2004 (Melanoma, Experimental) : Melanotic melanoma line evoked moderate increase secretion of all above mentioned substances, whereas amelanotic melanoma line, tumor with higher immunogenicity, tumorogenicity and with shorter survival time of animals induced considerably increase of IL-12 and NO with prominent decrease of IL-10 secretion
Rigby et al., Clin Exp Immunol 2005 : In contrast, colonic DC produced IL-12 in response to both LPS and B.longum
Elkord et al., Immunology 2005 : In this study, we show that the positive selection of monocytes by anti-CD14 coated microbeads inhibits the lipopolysaccharide (LPS) induced production of interleukin (IL)-12 , IL-10 and tumour necrosis factor-alpha (TNF-alpha) from human DC
Cohen et al., Psychosom Med 2005 (Breast Neoplasms) : Spontaneous and interleukin-2 (IL-2) induced NCA, in vitro IL-2 and IL-12 secretion, and levels of plasma cortisol and urinary catecholamines were tested in daughters
Cuéllar et al., Biomedica 2004 : The dendritic cells pre exposed to single doses of endotoxin demonstrated a reduced capacity to mature, reduced CD83 expression, inhibited secretion of IL-12 , TNFalpha, IL-10 and diminished secretion of IL-6 ... The dendritic cells pre exposed to single doses of endotoxin demonstrated a reduced capacity to mature, reduced CD83 expression, inhibited secretion of IL-12 , TNFalpha, IL-10 and diminished secretion of IL-6
Mizuno et al., Nihon Rinsho Meneki Gakkai Kaishi 2004 (Salmonella Infections) : Especially, IL-12 , IL-15 and IL-18 play important roles in protection against Salmonella infection, proliferation of NK cell, NKT cell and gammadelta T cell, producing IFN-gamma, in addition, IL-12 and IL-18 induce IFN-gamma production by Th1 cells and adaptive immune response
Pedersen et al., Scand J Immunol 2005 (Breast Neoplasms) : Polyriboinosinic polyribocytidylic acid ( Poly I:C ) + tumour necrosis factor-alpha (TNF-alpha) induced significant IL-12 p70 secretion, which was increased after addition of a decoy IL-10 receptor
Rosa et al., Exp Parasitol 2005 (Leishmaniasis, Visceral) : In BALB/c mice, specific immune response to protein fractions was only observed at the higher parasitic level, with the fraction Inter promoting the production of IL-4 and fractions High and Low inducing high levels of IL-12
Li et al., Cancer Res 2005 (Fibrosarcoma) : IL-12 and IL-18 stimulated CD4 ( + ) TDLN cells and enhanced IFNgamma production by CD4 ( + ) cells to a greater extent than by CD8 ( + ) cells ... IL-12 and IL-18 stimulated CD4 ( + ) TDLN cells and enhanced IFNgamma production by CD4 ( + ) cells to a greater extent than by CD8 ( + ) cells ... Furthermore, a specific NF-kappaB inhibitor significantly suppressed IL-12/IL-18 induced IFNgamma secretion, thus confirming the requirement for NF-kappaB activation in IL-12/IL-18 signaling
Harimaya et al., FEMS Immunol Med Microbiol 2005 (Otitis Media) : The release of interleukin (IL)-12 was induced after stimulation with A. otitidis, whereas the release of IL-4 was not induced after stimulation with A. otitidis
Stanilova et al., Int Immunopharmacol 2005 : C3bgp also increased IL-12 in PHA or PWM stimulated cultures, but not in LPS stimulated culture
Agrawal et al., Int Immunol 2005 : MUC1 expression on activated T cells was increased in the presence of IL-12 but was not affected by IFN-gamma, IL-2, IL-4, IL-5, IL-10, IL-13 or TNF-alpha ... MUC1 expression on activated T cells was increased in the presence of IL-12 but was not affected by IFN-gamma , IL-2, IL-4, IL-5, IL-10, IL-13 or TNF-alpha ... MUC1 expression on activated T cells was increased in the presence of IL-12 but was not affected by IFN-gamma, IL-2, IL-4, IL-5, IL-10 , IL-13 or TNF-alpha ... MUC1 expression on activated T cells was increased in the presence of IL-12 but was not affected by IFN-gamma, IL-2, IL-4, IL-5, IL-10, IL-13 or TNF-alpha ... MUC1 expression on activated T cells was increased in the presence of IL-12 but was not affected by IFN-gamma, IL-2 , IL-4, IL-5, IL-10, IL-13 or TNF-alpha ... MUC1 expression on activated T cells was increased in the presence of IL-12 but was not affected by IFN-gamma, IL-2, IL-4 , IL-5, IL-10, IL-13 or TNF-alpha ... MUC1 expression on activated T cells was increased in the presence of IL-12 but was not affected by IFN-gamma, IL-2, IL-4, IL-5 , IL-10, IL-13 or TNF-alpha ... MUC1 expression on activated T cells was increased in the presence of IL-12 but was not affected by IFN-gamma, IL-2, IL-4, IL-5, IL-10, IL-13 or TNF-alpha
Stanilova et al., Intensive Care Med 2005 (Sepsis) : Stimulation with C3bgp , lipopolysaccharide, and pokeweed mitogen considerably reduced IL-12 production in nonsurvivors
Hamed et al., Egypt J Immunol 2003 (Carcinoma, Hepatocellular...) : We also evaluated interleukin (IL)-12 levels in these patients since IL-12 induces interferon (IFN)-gamma production
Rahim et al., Immunology 2005 : Interleukin-10 (IL-10) mediated suppression of IL-12 production in RAW 264.7 cells also involves c-rel transcription factor ... Interleukin-10 (IL-10) mediated suppression of IL-12 production in RAW 264.7 cells also involves c-rel transcription factor ... We demonstrate that the c-rel transcription factor also plays a major role in IL-10 mediated IL-12 suppression ... We demonstrate that the c-rel transcription factor also plays a major role in IL-10 mediated IL-12 suppression ... In a complementary experiment, in vitro transient expression of p65 NF-kappaB could not rescue the inhibitory effect of IL-10 on IL-12 production, suggesting that NF-kappaB alone was not sufficient to restore IL-12 production during IL-10 treatment ... In a complementary experiment, in vitro transient expression of p65 NF-kappaB could not rescue the inhibitory effect of IL-10 on IL-12 production, suggesting that NF-kappaB alone was not sufficient to restore IL-12 production during IL-10 treatment ... Our studies highlight the involvement of c-rel in IL-10 mediated IL-12 regulation
Kang et al., Cell Signal 2005 (Autoimmune Diseases) : IL-12 production is differentially regulated through multiple pathways, which can be classified as follows : nuclear factor-kappaB (NF-kappaB) and other transcription factors, p38 mitogen activated protein ( MAP ) kinase, cyclic adenosine monophosphate ( cyclic AMP ) -modulating molecules, cell membrane ion channels and pumps, nitric oxide ( NO ), and receptors
Traidl-Hoffmann et al., J Exp Med 2005 : Chemical analysis of Bet.-APE revealed the presence of phytoprostanes ( dinor isoprostanes ) with prostaglandin E ( 1 ) -, F ( 1 ) -, A ( 1 ) -, or B ( 1 ) -ring systems of which only E ( 1 ) -phytoprostanes dose dependently inhibited the LPS induced IL-12 p70 release and augmented the Th2 cell polarizing capacity of DC
Rodriguez-Galán et al., J Immunol 2005 : In the periphery, IL-18 synergistically induces the expression of the Th1 cytokine IFN-gamma in the presence of IL-12 and the Th2 cytokines IL-5 and IL-13 in the presence of IL-2 ... In the periphery, IL-18 synergistically induces the expression of the Th1 cytokine IFN-gamma in the presence of IL-12 and the Th2 cytokines IL-5 and IL-13 in the presence of IL-2 ... In the periphery, IL-18 synergistically induces the expression of the Th1 cytokine IFN-gamma in the presence of IL-12 and the Th2 cytokines IL-5 and IL-13 in the presence of IL-2 ... In contrast, IL-18-plus-IL-12 specifically induced CCR5 expression ... The IL-2-plus-IL-18 or IL-12-plus-IL-18 effect on IFN-gamma and IL-13 expression is dependent on Stat4 and NF-kappaB but independent of Stat6, T-bet, or NFAT ... The IL-2-plus-IL-18 or IL-12-plus-IL-18 effect on IFN-gamma and IL-13 expression is dependent on Stat4 and NF-kappaB but independent of Stat6, T-bet, or NFAT
Nakahara et al., Clin Exp Immunol 2005 (Neoplasms) : However, IL-1beta, but not IFN-gamma , induced IL-12 p40 production by DCs without enhancing phenotypic maturation ... However, IL-1beta , but not IFN-gamma, induced IL-12 p40 production by DCs without enhancing phenotypic maturation
Watanabe et al., Blood 2005 (Dermatitis, Atopic...) : Human TSLP promotes CD40 ligand induced IL-12 production by myeloid dendritic cells but maintains their Th2 priming potential ... Human TSLP promotes CD40 ligand induced IL-12 production by myeloid dendritic cells but maintains their Th2 priming potential
Lenert et al., J Clin Immunol 2005 (Disease Models, Animal...) : Moreover, the addition of purified B cells back to PN splenocyte cultures resulted in a B-cell number dependent/ IL-10 mediated suppression of IL-12p40
Smolarczyk et al., Cytokine 2005 : Negligible induction of IFN-gamma, IL-12 and TNF-alpha by DNA-PEI 750 kDa/albumin complexes
Entleutner et al., Int J Colorectal Dis 2006 (Disease Models, Animal...) : The induction of IFN-gamma by IL-12 is believed to play a key role in sepsis as it promotes antibacterial effector mechanisms such as oxidative burst or nitric oxide induction
Kandil et al., Clin Exp Immunol 2005 (Necrosis) : We investigated the regulation of interleukin (IL)-12 secretion by human DCs in response to tumour cell necrosis in an in vitro culture model ... Furthermore, priming DCs with necrotic cells augmented IL-12p70 secretion significantly in conjunction with CD40 cross linking
Monteleone et al., Gastroenterology 2005 (Colitis, Ulcerative...) : We also examined the effects of exogenous IL-12 on IL-21 production, as well as the effects of blocking IL-21 with an IL-21-receptor Ig fusion protein ... IL-12 enhanced IL-21 in normal lamina propria lymphocytes through an IFN-gamma independent mechanism, and blocking IL-12 in CD LPMCs decreased anti-CD3 stimulated IL-21 expression
Chen et al., Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2005 : MBL stimulated the production of IL-12 by MoDCs, but had no such effect on TNF-alpha secretion
Petro et al., Microbes Infect 2005 (Cardiovirus Infections) : U0126 decreased the IL-12 p35 response of SJL/J macrophages
Chino et al., Int Immunopharmacol 2005 (MAP Kinase Signaling System) : Treatment with chemical inhibitors of NF-kappa B and p38 mitogen activated protein kinases ( MAPKs ) in vitro inhibited LPS induced IL-12 production, whereas JNK and ERK inhibitors increased IL-12 production ... Treatment with chemical inhibitors of NF-kappa B and p38 mitogen activated protein kinases ( MAPKs ) in vitro inhibited LPS induced IL-12 production, whereas JNK and ERK inhibitors increased IL-12 production
Takatori et al., J Immunol 2005 : We also found that IL-12 induced Stat4 phosphorylation and Th1 cell differentiation were augmented in Stat5a ( -/- ) CD4 ( + ) T cells ... Importantly, the expression of suppressor of cytokine signaling (SOCS)3, a potent inhibitor of IL-12 induced Stat4 activation, was decreased in Stat5a ( -/- ) CD4 ( + ) T cells
Kim et al., J Immunol 2005 (Infection...) : Chemical inhibition of stress activated protein kinase/JNK showed that this MAPK was also required for parasite triggered IL-12 production
Szkaradkiewicz et al., Antiviral Res 2005 (Hepatitis B, Chronic) : However, the most pronounced stimulatory effect was observed in the presence of IL-12+IL-18 and resulted in peak levels of IFN-gamma production
Leung et al., J Biomol Screen 2005 : The authors were interested in identifying nonpeptide inhibitors of the Th1 response selective for the IL-12/IL-18 mediated secretion of IFN-gamma while leaving the IL-4 mediated Th2 cytokine secretion relatively intact ... The authors established a screening protocol using human peripheral blood mononuclear cells ( PBMCs ) and identified the hydrazino anthranilate compound 1 as a potent inhibitor of IL-12/IL-18 mediated IFN-gamma secretion from CD3 ( + ) cells with an IC ( 50 ) around 200 nM
Smeltz et al., Immunology 2005 : T cells primed with TGF-beta secreted IFN-gamma in response to both T-cell receptor ligation and IL-12/IL-18 stimulation, and expressed high levels of T-bet and low levels of GATA-3
Feder-Mengus et al., Hum Gene Ther 2005 (Neoplasms) : Most importantly, IL-12 p40 gene expression and protein secretion were induced by CD154rVV but not by wild-type VV ( WT VV ) in either CD14+ cells or iDC, and these effects could be blocked by anti-CD40 monoclonal antibodies
Shirota et al., J Immunol 2005 (Shock, Septic) : Underlying this protective effect is the ability of suppressive ODN to bind to and prevent the phosphorylation of STAT1 and STAT4, thereby blocking the signaling cascade mediated by LPS induced IFN-beta and IL-12
Finkelman et al., J Immunol 2005 (Bronchial Hyperreactivity) : Although IL-4, but not IL-13, induces IL-12 and IFN-gamma, which suppress many effects of IL-4, IL-12 suppresses only the Ca2T6 gene, and IL-4 induced IFN-gamma production does not suppress the Ca2T6 or SPRR genes ... Although IL-4, but not IL-13 , induces IL-12 and IFN-gamma, which suppress many effects of IL-4, IL-12 suppresses only the Ca2T6 gene, and IL-4 induced IFN-gamma production does not suppress the Ca2T6 or SPRR genes ... Although IL-4 , but not IL-13, induces IL-12 and IFN-gamma, which suppress many effects of IL-4, IL-12 suppresses only the Ca2T6 gene, and IL-4 induced IFN-gamma production does not suppress the Ca2T6 or SPRR genes
Tournier et al., J Immunol 2005 : Furthermore, ET inhibits in vivo IL-12p70 and IFN-gamma secretion induced by LPS
Papadakis et al., J Immunol 2005 (Crohn Disease) : The TNF-like cytokine TL1A augments IFN-gamma production by anti-CD3 plus anti-CD28 and IL-12/IL-18 stimulated peripheral blood (PB) T cells
Robertson et al., Blood 2005 (Lymphoma) : Interleukin-12 (IL-12) induced IFN-gamma production is markedly defective after autologous stem cell transplantation
Wilmanski et al., J Leukoc Biol 2005 (Disease Models, Animal...) : Lipopolysaccharide (LPS) induced interleukin (IL)-10 and IL-12 production was doubled in G6PD-deficient macrophages compared with wild-type ( WT )
Hornyak et al., Vascul Pharmacol 2005 (Capillary Leak Syndrome...) : Serum levels of IL-1beta, IL-4 and IL-12 were not measurably induced by IL-2 treatment
Correa et al., Br J Pharmacol 2005 : Activation of cannabinoid CB2 receptor negatively regulates IL-12p40 production in murine macrophages : role of IL-10 and ERK1/2 kinase signaling ... Macrophages infected with TMEV increased IL-12p40 production and activation of CB2 receptors by JWH-133 ( 100 nM ) inhibited it ... 3 The inhibitory effect of JWH-133 ( 100 nM ) on IL-12p40 production may involve extracellular regulated kinase ( ERK1/2 ) signaling : ( i ) JWH-133 induced a greater and sustained activation of ERK1/2 kinase in comparison with the level of activation observed following LPS ; ( ii ) the inhibition of ERK1/2 by the specific inhibitor PD98059 increased LPS induced IL-12p40 production in the presence or absence of JWH-133 suggesting a negative regulation of ERK pathway on IL-12p40 biosynthesis ... 3 The inhibitory effect of JWH-133 ( 100 nM ) on IL-12p40 production may involve extracellular regulated kinase ( ERK1/2 ) signaling : ( i ) JWH-133 induced a greater and sustained activation of ERK1/2 kinase in comparison with the level of activation observed following LPS ; ( ii ) the inhibition of ERK1/2 by the specific inhibitor PD98059 increased LPS induced IL-12p40 production in the presence or absence of JWH-133 suggesting a negative regulation of ERK pathway on IL-12p40 biosynthesis ... Endogenous IL-10 plays a modulatory role in IL-12 production ... In contrast, the addition of exogenous mIL-10 reduced the secretion of IL-12p40 in response to LPS
Kang et al., Arch Pharm Res 2005 : Myricetin significantly inhibited the LPS induced IL-12 production from both primary macrophages and the RAW264.7 monocytic cell-line in a dose dependent manner
Schuetze et al., Int Immunol 2005 : Whereas p19 was induced early and transiently, p40 and p35 were up-regulated later and then continuously, but the secretion of IL-23 and IL-12p70 was significantly reduced by IL-10
Xiong et al., J Biol Chem 2005 : Furthermore, IRF-8 induced IL-12 p40 synthesis in RAW264.7 cells was enhanced by dominant negative Cbl , and peritoneal macrophages from Cbl-/- mice showed increased IL-12 p40 protein production ... Furthermore, IRF-8 induced IL-12 p40 synthesis in RAW264.7 cells was enhanced by dominant negative Cbl, and peritoneal macrophages from Cbl-/- mice showed increased IL-12 p40 protein production ... Furthermore, IRF-8 induced IL-12 p40 synthesis in RAW264.7 cells was enhanced by dominant negative Cbl, and peritoneal macrophages from Cbl-/- mice showed increased IL-12 p40 protein production
Gutzmer et al., J Immunol 2005 : Histamine H4 receptor stimulation suppresses IL-12p70 production and mediates chemotaxis in human monocyte derived dendritic cells ... H4R or H2R stimulation suppressed IL-12p70 production in MoDC ... H4R or H2R stimulation suppressed IL-12p70 production in MoDC ... In contrast, H4R stimulation did not affect cAMP production but induced the transcription factor AP-1, and U0126, an inhibitor of AP-1 transactivation and MEK, rescued H4R mediated IL-12p70 suppression
Gautier et al., J Exp Med 2005 : Dendritic cells ( DC ) produce interleukin-12 (IL-12) in response to Toll-like receptor ( TLR ) activation ... Moreover, analysis of interferon regulatory factors ( IRF ) regulation in moDC suggests a role for IRF7/8 in mediating IRF3 independent type I IFN and possibly IL-12p35 synthesis in response to TLR7/8 ... Moreover, analysis of interferon regulatory factors ( IRF ) regulation in moDC suggests a role for IRF7/8 in mediating IRF3 independent type I IFN and possibly IL-12p35 synthesis in response to TLR7/8
Renkl et al., Blood 2005 : OPN induces their Th1 promoting tumor necrosis factor alpha (TNF-alpha) and interleukin-12 (IL-12) secretion, and enhances their allostimulatory capacity ... In mixed lymphocyte reactions ( MLRs ), OPN stimulates IL-12 secretion by DCs, inducing elevated interferon-gamma (IFN-gamma) production by T cells
Yarovinsky et al., Science 2005 (Toxoplasmosis, Animal) : Moreover, TLR11 is required in vivo for parasite induced IL-12 production and optimal resistance to infection, thereby establishing a role for the receptor in host recognition of protozoan pathogens
Methe et al., Arterioscler Thromb Vasc Biol 2005 : Statin incubation resulted in a dose dependent reduction of TLR4 expression ( 53+/-7.6 % reduction compared with untreated monocytes ; P < 0.005 ), transcript levels ( 68+/-6.3 % ; P < 0.002 ), decreased IRAK phosphorylation ( 37+/-8.3 % ; P < 0.05 ), and LPS induced IL-6, IL-12 , tumor necrosis factor (TNF)-alpha, and B7-1 expression ( P < 0.05 )
Kato et al., Pathobiology 2005 : T-cell activity and the level of IFN-gamma, IL-10 and IL-12 expression in rp30 immunized T cells were considerably increased in the presence of rp30 Ags
Inomata et al., Cytokine 2005 (Disease Progression...) : Circulating IFN-gamma, IL-12 , IL-18 and OPN paralleled the extent of lung lesions, and circulating IFN-gamma , IL-18 and OPN paralleled the magnitude of fever in patients with pulmonary tuberculosis
Walker et al., J Pediatr Surg 2005 (Disease Models, Animal...) : Only CD40 dependent IL-12 production was abrogated ( 60 %, P < .01 ) in DC derived from NB-bearing mice
Lu et al., Cytokine 2005 : Co-transfection studies showed that HDAC1 had a repressing effect on the activity of IL-12 p40 promoter
Gruber et al., Cancer Gene Ther 2005 (Philadelphia Chromosome...) : We found that IL-12 was able to eliminate pre established leukemia, and that this protection was mediated by CD4 , CD8, and NK cells in combination ... We found that IL-12 was able to eliminate pre established leukemia, and that this protection was mediated by CD4, CD8 , and NK cells in combination
Buhtoiarov et al., J Immunol 2005 (Leukemia L5178...) : IFN-gamma production by macrophages was dependent on IL-12
Tsai et al., Cytokine 2005 (Periodontal Diseases) : IL-12 is an inducer of INF-r production ... IFN-gamma itself can also activate IL-12 production ... Lipopolysaccharides (LPS) of periodontopathogens are also activators of IL-12
Mu et al., Cell Microbiol 2005 : Further, blockade of TLR4 on macrophages of C3H/HeN mice with antibody greatly increased expression of TLR2 and release of IL-12p40 in response to MAM
Kim et al., Immunol Lett 2005 : HQ potently inhibited the LPS induced IL-12 production in both primary mouse macrophages and RAW164.7 monocytic cells in a dose dependent manner
Tenca et al., J Immunol 2005 (Calcium Signaling) : Likewise, CD85j stimulation strongly reduced IL-8 and IL-12 production by hOSCAR activated DCs
Harris et al., J Immunol 2005 : B cells produce IFN-gamma in response to IL-12 and IL-18 and when primed by Th1 cells ... Finally, we show that although IL-12/IL-18 or IFN-gamma producing Th1 cells are required to initiate transcription of the IFN-gamma gene in B cells, sustained expression of IFN-gamma and T-bet by B cells is dependent on an IFN-gamma/IFN-gammaR/T-bet autocrine feedback loop
Filatenkov et al., J Immunol 2005 : In the presence of CD4 T cells, Ag and B7 levels do not increase on dendritic cells but IL-12 production is up-regulated, and this requires CD154 expression on the CD4 T cells
Maldonado-Bernal et al., Parasite Immunol 2005 : The interaction of LPPG with TLR2 and TLR4 resulted in activation of NF-kappaB and release of interleukin (IL)-10, IL-12p40 , tumour necrosis factor (TNF)-alpha, and IL-8 from human monocytes ... The interaction of LPPG with TLR2 and TLR4 resulted in activation of NF-kappaB and release of interleukin (IL)-10, IL-12p40 , tumour necrosis factor (TNF)-alpha, and IL-8 from human monocytes
Burger-Kentischer et al., Atherosclerosis 2006 (Aortitis...) : Importantly, the local aortic expression of ICAM-1, MMP-2, TNF, IL-12 , and CD40L was reduced by MIF blockade, as were the levels of the phospho-c-Jun and C/EBPbeta transcription factors
Dumitriu et al., J Immunol 2005 : This secreted HMGB1 is necessary for the up-regulation of CD80, CD83, and CD86 surface markers of human dendritic cells and for IL-12 production
Patel et al., J Immunol 2005 (Respiratory Hypersensitivity) : However, the anti-inflammatory effect of Pam3CSK4 was independent of IL-10 or TGF-beta, but was critically dependent on IL-12 , the production of which by dendritic cells was enhanced by Pam3CSK4 in vitro ... However, the anti-inflammatory effect of Pam3CSK4 was independent of IL-10 or TGF-beta, but was critically dependent on IL-12 , the production of which by dendritic cells was enhanced by Pam3CSK4 in vitro
Puddu et al., J Leukoc Biol 2005 : In contrast, endogenous IFN-gamma was requested for the IL-12 induced IFN-gamma production
Cheng et al., J Hepatol 2005 : rHBsAg was also found to inhibit the induction of IL-12 by LPS in THP-1 cells ... These results showed a novel anti-inflammatory property of rHBsAg which involves inhibition of COX-2 and suggested that hepatitis B virus may regulate IFN-gamma production by inhibiting IL-18 and IL-12 production
Biet et al., Allergy 2005 (Eosinophilia...) : The induction of IFN-gamma is mediated by interleukin (IL)-12 known to be secreted upon mycobacterial infections and can be enhanced by IL-18 acting in synergy with IL-12
Xu et al., Gene Ther 2005 (Colonic Neoplasms...) : We have previously shown that the local-membrane bound 4-1BB ligand and IL-12 gene transfer induced a significant antitumor response in a mouse colon carcinoma model
Takasaki et al., Kansenshogaku Zasshi 2005 (Cerebral Palsy...) : IL-12 induces production of interferon-gamma , which enhances the function of polymorphonuclear leukocytes
Yadavalli et al., Shock 2005 (Postoperative Complications) : We conducted a prospective, observational study to test whether cardiac surgery additionally impaired dendritic and natural killer ( NK ) cell functions responsible for innate immune production of interleukin (IL)-12 dependent interferon (IFN)-gamma in response to bacteria or toll-like receptor agonists
Slade et al., Expert Opin Investig Drugs 1998 : The induction of tumour necrosis factor alpha (TNF-alpha), interferon gamma (IFN-gamma) and interleukin-12 (IL-12) leads to inhibition of IL-5 , with animal models demonstrating immune deviation away from Th2 immune responses
Mizuno et al., Immunol Lett 2005 : Beta2-adrenergic receptor stimulation inhibits LPS induced IL-18 and IL-12 production in monocytes ... Beta2-adrenergic receptor stimulation inhibits LPS induced IL-18 and IL-12 production in monocytes ... IL-12 production induced by LPS was inhibited by anti-IL-18 Ab, but IL-18 production by LPS was not inhibited by anti-IL-12 Ab, showing that LPS induced IL-18 production without IL-12 production ... IL-12 production induced by LPS was inhibited by anti-IL-18 Ab, but IL-18 production by LPS was not inhibited by anti-IL-12 Ab, showing that LPS induced IL-18 production without IL-12 production
King et al., J Immunol 2005 : Cutting edge : IL-12 induces CD4+CD25- T cell activation in the presence of T regulatory cells ... Cutting edge : IL-12 induces CD4+CD25- T cell activation in the presence of T regulatory cells
Kang et al., J Immunol 2005 : The costimulation of DCs with WKYMVm and LPS dramatically inhibited LPS induced IL-12 production, CD86 and HLA-DR surface expression, and DC-mediated T cell proliferation ... The costimulation of DCs with WKYMVm and LPS dramatically inhibited LPS induced IL-12 production, CD86 and HLA-DR surface expression, and DC-mediated T cell proliferation ... Moreover, the costimulation of DCs with WKYMVm and LPS dramatically inhibited the LPS induced accumulations of IL-12 mRNA, thus suggesting that WKYMVm inhibits LPS induced IL-12 production at the transcriptional level ... In addition, formyl-Met-Leu-Phe ( a FPR ligand ), Trp-Lys-Tyr-Met-Val-Met , Hp ( 2-20 ) peptide, and F2L ( three FPRL2 ligands ) inhibited LPS induced IL-12 production in DCs ... In addition, formyl-Met-Leu-Phe ( a FPR ligand ), Trp-Lys-Tyr-Met-Val-Met , Hp ( 2-20 ) peptide, and F2L ( three FPRL2 ligands ) inhibited LPS induced IL-12 production in DCs ... In addition, formyl-Met-Leu-Phe ( a FPR ligand ), Trp-Lys-Tyr-Met-Val-Met , Hp ( 2-20 ) peptide, and F2L ( three FPRL2 ligands ) inhibited LPS induced IL-12 production in DCs ... In addition, formyl-Met-Leu-Phe ( a FPR ligand ), Trp-Lys-Tyr-Met-Val-Met, Hp ( 2-20 ) peptide, and F2L ( three FPRL2 ligands ) inhibited LPS induced IL-12 production in DCs
Boyd et al., J Immunol 2005 : The wild-type acylated toxin ( A-CyaA ) and nonacylated CyaA ( NA-CyaA ), but not CyaA with an inactive adenylate cyclase domain ( iAC-CyaA ), enhanced TLR-ligand induced IL-10 and inhibited IL-12 , TNF-alpha, and CCL3 production by macrophages and DC
Khader et al., J Immunol 2005 (Genetic Predisposition to Disease...) : IL-12p70 induced IFN-gamma is required to control Mycobacterium tuberculosis growth ; however, in the absence of IL-12p70, an IL-12p40 dependent pathway mediates induction of IFN-gamma and initial bacteriostatic activity
Wilson et al., J Neuroimmunol 2005 (Chronic Disease...) : Endogenous levels of IL-10 inhibited the production of IL-12 , IFN-gamma, TNF-alpha, and IL-6 from both hematopoetic and non-hematopoetic cells in the brain, as well as anti-microbial activity of astrocytes
Lehner et al., Immunol Lett 2005 : However, IL-12p70 production in response to Toll-like receptor ( TLR ) stimulating agents plus IFN-gamma was consistently lower in AIMV medium although also under serumfree culture conditions, nanogram quantities of IL-12 were produced
Satwani et al., Br J Haematol 2005 : In the present study, we compared IL-18 gene expression and protein production and IL-18 mRNA half-life in basal versus activated CB versus PB MNCs, the effects of IL-18 +/- IL-12 on MNCs IFN-gamma protein production and ex vivo expansion and activation of CB with IL-12 + IL-2 + anti-CD3 +/- IL-18 ... IL-18 synergistically with IL-12 induced IFN-gamma production from PB greater than CB MNCs ( P < 0.05 )
Thomas et al., Arch Toxicol 2005 : In this study we address the effects of IL-2 and IL-12 on the TBT induced decreases in NK-cell levels of the cytotoxic proteins, their mRNAs, and CREB phosphorylation
Pietilä et al., J Leukoc Biol 2005 (Salmonella Infections) : CC chemokine ligand 5 (CCL5), CXC chemokine ligand 10, tumor necrosis factor alpha, interleukin (IL)-12 , and IL-18 gene expression and protein production were readily induced by Salmonella infected Mphis and DCs
Berg et al., J Immunol 2005 (Listeriosis) : We have shown recently that CD8 T cells have the ability to rapidly secrete IFN-gamma independent of Ag, in response to IL-12 and IL-18, during a LM infection
Ireland et al., Viral Immunol 2005 (Encephalitis, Viral...) : Furthermore, these data indicate that IL-23 is not acting redundantly in the absence of IL-12 during VSV induced encephalitis
Ellermann-Eriksen et al., Virology journal 2005 (Herpes Simplex) : In the next wave, interleukin (IL)-12 together with the above and other cytokines induce production of IFN-gamma in mainly NK cells
Yap et al., FEBS Lett 2005 : STAT4 is a transcription factor activated in response to IL-12 , and is involved in Th1 cell development
Tsukada et al., Int Immunol 2005 : The CD8 DCs secrete a large amount of IFN-gamma, IL-12 and B lymphocyte stimulator/B cell activation factor of the tumor necrosis factor family in response to LPS and CpG stimulation
Yue et al., Proc Natl Acad Sci U S A 2005 : This study demonstrates that CD1d ligation alone, in the absence of iNKT, could rapidly ( within 24 h ) stimulate production of bioactive IL-12p70 by CD1d+ human peripheral blood monocytes as well as iDCs ... IFNgamma alone had no effect, but it markedly enhanced CD1d stimulated IL-12 production ... IFNgamma alone had no effect, but it markedly enhanced CD1d stimulated IL-12 production ... CD1d ligation on the human monocytic cell line THP-1 similarly specifically stimulated IL-12 production ... Selective NF-kappaB inhibition blocked this CD1d stimulated IL-12 production ... Selective NF-kappaB inhibition blocked this CD1d stimulated IL-12 production ... Finally, CD1d ligation could also enhance IL-12 production in the presence of suboptimal LPS or CD40 stimulation ... Finally, CD1d ligation could also enhance IL-12 production in the presence of suboptimal LPS or CD40 stimulation ... Finally, CD1d ligation could also enhance IL-12 production in the presence of suboptimal LPS or CD40 stimulation ... Finally, CD1d ligation could also enhance IL-12 production in the presence of suboptimal LPS or CD40 stimulation
Sato et al., Gut 2005 (Colitis, Ulcerative...) : Recent studies have shown that OPN plays an important role in lymphocyte migration, granuloma formation, and interleukin 12 (IL-12) production ... OPN induced production of IL-12 from LPMC in CD but not in normal controls or UC
Ge et al., Arterioscler Thromb Vasc Biol 2005 (Atherosclerosis...) : AGE-BSA induced expression of CD83 and secretion of IL-12 were partly inhibited by either an anti-RAGE neutralizing antibody or a Jnk inhibitor
Iacobelli-Martinez et al., J Virol 2005 (Adenoviridae Infections) : Subgroup B ( Ad16 and -35 ) and Ad37, but not Ad2 or -5, significantly reduced IL-12 production by human peripheral blood mononuclear cells stimulated with gamma interferon ( IFN-gamma ) and lipopolysaccharide
Kikuchi et al., J Dent Res 2005 (Periodontitis) : Although P. gingivalis is not a potent inducer of Th1 responses, it stimulated strong IL-12 responses by monocyte derived dendritic cells in the presence of IFN-gamma , and IFN-gamma was produced by NK cells within 24 hrs in the presence of dendritic cells
Jongmans et al., J Immunother 2005 (Inflammation) : No IL-12p70 could be detected when DCs were stimulated with TNF-alpha/PGE2 combined with MCM or IL-1beta/IL-6, presumably by suppression by PGE2
Markiewicz et al., J Immunol 2005 : In addition, both RAE1epsilon and B7-1 enhanced Ag-independent IFN-gamma secretion in response to IL-12 and IL-18 by DUC CTL
Dai et al., J Immunol 2005 : Gal-9 induced phosphorylation of the MAPK p38 and ERK1/2 in DCs, and an inhibitor of p38 signaling, but not inhibitors of signaling by either ERK1/2 or PI3K, blocked Gal-9 induced up-regulation of costimulatory molecule expression and IL-12 production ... Gal-9 induced phosphorylation of the MAPK p38 and ERK1/2 in DCs, and an inhibitor of p38 signaling, but not inhibitors of signaling by either ERK1/2 or PI3K, blocked Gal-9 induced up-regulation of costimulatory molecule expression and IL-12 production
Boyer et al., J Med Primatol 2005 (Monkey Diseases...) : The adjuvanted DNA vaccines illustrate that IL-12 enhances a CD8 vaccine immune response, however, different cellular profiles
An et al., Inflammation 2004 : In this study, using mouse peritoneal macrophages, we have examined whether XF affects nitric oxide ( NO ), tumor necrosis factor (TNF)-alpha, and interleukin (IL)-12p40 production induced by interferon (IFN)-gamma and lipopolysaccharide (LPS) ... In this study, using mouse peritoneal macrophages, we have examined whether XF affects nitric oxide ( NO ), tumor necrosis factor (TNF)-alpha, and interleukin (IL)-12p40 production induced by interferon (IFN)-gamma and lipopolysaccharide (LPS)
Pritchard et al., Immunol Invest 2005 : This effect does not appear to depend on differences in IL-12 induced IFN-gamma , but may involve production of nitric oxide ( NO ), since treatment of mice with a NOS inhibitor, NG-monomethyl-L-arginine ( L-NMA ), abolishes the additive anti-tumor effect of the combination treatment
Xie et al., Acta Biochim Biophys Sin (Shanghai) 2005 (Carcinoma, Hepatocellular...) : Among these differentially expressed genes, p16INK4, IL-12 , TRAIL and the lymphotoxin beta gene involved in growth suppression or cell apoptosis were up-regulated, and PTEN involved in cell proliferation was down-regulated by BNIPL-1
Kojo et al., J Immunol 2005 (Encephalomyelitis, Autoimmune, Experimental) : After repeated injection of alpha-galactosylceramide ( alpha-GalCer ) into mice, splenic DCs acquired properties of regulatory DCs in IL-10 dependent fashion, such as nonmatured phenotypes and increased IL-10 but reduced IL-12 production
Thien et al., J Allergy Clin Immunol 2005 : 1 alpha,25 ( OH ) ( 2 ) D ( 3 ) had little effect on T ( H ) /T(C)1 cytokines but significantly inhibited IL-12 induced IFN-gamma production
Dercamp et al., Cancer Res 2005 (Thymoma) : Similarly, both IL-10 and T ( reg ) were responsible for impaired CD8 T cell priming by TIDCs, but IL-12 production by TIDCs was prevented only by T ( reg ) -independent IL-10
Waggoner et al., J Immunol 2005 : We report the selective inhibition of TLR4 induced IL-12 production after cross linking of gC1qR on the surface of macrophages and dendritic cells ... Suppression of IL-12 did not result from increased IL-10 or TGF-beta, but was dependent on PI3K activation ... Suppression of IL-12 did not result from increased IL-10 or TGF-beta, but was dependent on PI3K activation
Patel et al., J Immunol 2005 (IgG Deficiency...) : The anti-IFN-gamma IgG had in vitro biological activity on the IFN-gamma dependent phosphorylation of STAT-1 as well as on the IFN-gamma dependent up-regulation of TNF-alpha and IL-12
Stewart et al., Infect Immun 2005 (Tuberculosis) : Delta19 induced lower interleukin 1beta (IL-1beta) secretion from monocytes and MDM. Overexpression of the 19-kDa protein increased IL-1beta, IL-12p40 , and tumor necrosis factor alpha secretion irrespective of phagocyte maturity
Ali et al., Circ Res 2005 (Inflammation) : Importantly, reconstitution of apoE expression in the liver of apoE-deficient mice normalized the LPS induced plasma protein levels of IL-12p40
Jenkins et al., Int Immunol 2005 : Experiments using TLR4-/- mice confirmed that IL-12p40 production by tM in response to 0-3hRP was partly dependent upon functional TLR4 , whereas IL-6 production was entirely independent ... Finally, we show that glycan components of 0-3hRP are required for optimal cytokine production since protease treatment of 0-3hRP had no effect on IL-12p40 production and only a slight effect on IL-6 , while sodium meta-periodate treatment almost completely abolished production of both cytokines
Loscher et al., J Immunol 2005 : Treatment of DC with c9, t11-CLA suppressed LPS induced IL-12 , enhanced IL-10R expression, and enhanced IL-10 production at the transcriptional and protein level ... Our findings demonstrate that c9, t11-CLA suppresses IL-12 production by LPS stimulated DC by ERK mediated IL-10-induction ... This is the first study to demonstrate that c9, t11-CLA can enhance transcription and production of the anti-inflammatory cytokine IL-10 , while inhibiting the Th1 promoting cytokine IL-12 , and may explain certain of its immunosuppressive properties
Zakharova et al., J Immunol 2005 (Listeriosis) : TNFR1 was established as the main receptor involved in IL-12p40 regulation, because IL-12p40 levels were not affected by TNF-alpha in TNFR1 ( -/- ) -derived macrophages
Wüthrich et al., J Immunol 2005 (Blastomycosis...) : CD4 ( + ) T cells absolutely required IL-12 to control a live genetically engineered attenuated strain of Blastomyces dermatitidis given s.c. as a vaccine, whereas CD8 ( + ) T cells were significantly less dependent on IL-12
Pearce et al., Prog Allergy 2006 : Polarized Th1 responses are induced by DCs , which respond to pathogen derived TLR ligands to mature and produce IL-12 and related cytokines that are instrumental in Th1 cell outgrowth
Goriely et al., Blood 2006 : Interferon regulatory factor 3 is involved in Toll-like receptor 4 (TLR4)- and TLR3 induced IL-12p35 gene activation ... Interferon regulatory factor 3 is involved in Toll-like receptor 4 (TLR4)- and TLR3 induced IL-12p35 gene activation ... Since IL-12p35 mRNA was induced in human DCs activated through TLR3 or TLR4 but not TLR2, we investigated the potential role of interferon regulatory factor 3 (IRF-3) in IL-12p35 gene transactivation ... Finally, experiments on DCs from IRF-3-deficient mice established that TLR4 induced IL-12p35 mRNA and IL-12p70 synthesis are impaired in absence of IRF-3 ... Finally, experiments on DCs from IRF-3-deficient mice established that TLR4 induced IL-12p35 mRNA and IL-12p70 synthesis are impaired in absence of IRF-3
Yentür et al., J Neuroimmunol 2005 (Subacute Sclerosing Panencephalitis) : There was a trend towards a decrease in IL-12 production in response to MBP and in vitro IL-12 secretion of SSPE patients to measles virus vaccine ( MVV ) was lower than controls ... Proliferation, as well as IFN-gamma, IL-12 and IL-10 production in response to purified protein derivate (PPD) was impaired in SSPE patients
Suto et al., J Immunol 2005 : We also found that IL-4 induced IFN-gamma production, but not IL-12 or IFN-alpha production, in PDCs and also strongly enhanced CpG oligodeoxynucleotide induced IFN-gamma production, but not CpG oligodeoxynucleotide induced IL-12 or IFN-alpha production
Chen et al., Blood 2006 : Of interest, DCAL-2 ligation had the opposite effects on TLR versus CD40L signaling : anti-DCAL-2 suppressed TLR induced IL-12 expression, but significantly enhanced CD40L induced IL-12 production
Varma et al., Infect Immun 2005 : LPS primed wild-type mice exhibited significant suppression of LPS induced IFN-gamma and IL-12 but increased IL-10 production in blood and spleen compared to levels exhibited by saline primed wild-type mice
Khan et al., Blood 2006 : Hydrogen peroxide inhibits IL-12 p40 induction in macrophages by inhibiting c-rel translocation to the nucleus through activation of calmodulin protein ... We now provide evidence that hydrogen peroxide ( H2O2 ), a major component of ROSs, inhibits interleukin-12 (IL-12) p40 and IL-12 p70 induction in murine macrophages and catalase pretreatment prevents H2O2 mediated down-regulation of IL-12 ... Endogenous accumulation of H2O2/ROSs in macrophages treated with alloxan resulted in IL-12 p40 inhibition
Deveci et al., Mediators Inflamm 2005 (Tuberculosis, Pulmonary) : It has been reported that IFN-gamma, TNF-alpha, and IL-12 stimulate, and that IL-10 , TGF-beta, and IL-4 suppress the protective immune response against tuberculosis ... It has been reported that IFN-gamma, TNF-alpha, and IL-12 stimulate, and that IL-10, TGF-beta, and IL-4 suppress the protective immune response against tuberculosis
Dodeller et al., Eur J Immunol 2005 : In contrast, inhibition of p38 activity did not affect expression of the Th1 cytokines IFN-gamma and TNF induced by TCR-stimulation, but decreased IL-12 mediated IFN-gamma expression
Pathak et al., J Biol Chem 2005 : Man-LAM inhibits LPS induced IL-12 p40 expression in an IL-10 independent manner ... Knockdown of Irak-M expression by RNA interference reinstates LPS induced IL-12 production in Man-LAM pretreated cells
Chen et al., J Immunol 2005 : Thymus CD161-CD44lowCD4+CD8- T cells produce low and no IFN-gamma inducibility in response to TCR stimulation and to IL-12 + IL-18, respectively, and they express diverse complementarity determining region 3 sequences for both TCR-alpha- and -beta-chains
Yun et al., Int Immunopharmacol 2005 : Furthermore, an electrophoretic mobility shift assay showed that CHL inhibited DNA binding activity of NF-kappaB, STAT-3, and STAT-4 to their cognate DNA recognition motifs, all of which contribute to the IL-12 induced IFN-gamma transcription
Ito et al., J Exp Med 2005 : Here we report that TSLP induced human DCs to express OX40 ligand (OX40L) but not IL-12
Mahieu et al., Pediatr Crit Care Med 2005 (Respiratory Insufficiency) : Pathogen induced excessive production of the proinflammatory cytokine TNF-alpha and lack of antibacterial IL-12 p 70 response in the TA are associated with increased neonatal mortality among ventilated preterm infants
Zeyda et al., Transplantation 2005 : In line, LPS- and CD40L induced upregulation of DC surface activation markers and production of IL-12 was significantly inhibited, irrespective of whether cells were treated during or after the monocyte to DC differentiation period
Ito et al., Int Immunol 2005 : In the presence of exogenously added IFN-beta , IL-12 p40 and IL-12 p70 production was inhibited after LSO stimulation, but IL-12 p70 production was enhanced after PGN stimulation
Härtel et al., Clin Exp Immunol 2005 : As information on immune cell responses in infancy and early childhood is scarce, we conducted an observational, cross-sectional study in healthy newborns ( n = 18 ), infants and young children ( n = 54 ) aged 1-96 months and adult controls ( n = 19 ) to assess cytokine mRNA and protein expression upon phorbol 12-myristate 13-actate/ionomycin stimulation and LPS induced IL-12 expression in monocytes
Tamir et al., Clin Exp Immunol 2005 : Under these circumstances, IFN-alpha2a did not modify the DC phenotype, and the production of IL-10/IL-12 and IFN-gamma/IL-5 by DCs and by DC-stimulated naive T cells, respectively, was inhibited compared to the effects on DCs treated with maturation factors alone
Wang et al., Clin Exp Immunol 2005 (Chlamydia Infections...) : Interleukin (IL)-2 and IL-12 responses to Chlamydia trachomatis infection in adolescents
Tada et al., Infect Immun 2005 : These findings suggest that Nod1 and Nod2 agonists in combination with TLR3, TLR4, and TLR9 agonists synergistically induce IL-12 and IFN-gamma production in DCs to induce Th1-lineage immune responses
Shin et al., J Immunol 2005 (Asthma) : STAT4, which plays a pivotal role in Th1 immune responses, enhances IFN-gamma transcription in response to the interaction of IL-12 with the IL-12R
Roggen et al., Toxicol Lett 2006 (Hypersensitivity) : PGE ( 2 ) is synergistic with IL-1beta and TNF-alpha in the induction of functional and phenotypic maturation of DC and induce IL12 p40 production ... PGE ( 2 ) is synergistic with IL-1beta and TNF-alpha in the induction of functional and phenotypic maturation of DC and induce IL12 p40 production
Jozefowski et al., Immunology 2005 : Consistent with the effects of leukotriene antagonists, exogenous LTB ( 4 ) enhanced LPS stimulated IL-10 release by approximately 39 % and inhibited IL-12 p40 release by approximately 22 % ... Consistent with the effects of leukotriene antagonists, exogenous LTB ( 4 ) enhanced LPS stimulated IL-10 release by approximately 39 % and inhibited IL-12 p40 release by approximately 22 %
Perl et al., Am J Pathol 2005 (Disease Models, Animal...) : Only Fas-siRNA markedly diminished lung tissue tumor necrosis factor-alpha, IL-6, IL-10, interferon-gamma, IL-12 , and caspase-3 activity
Eisenbeis et al., J Clin Oncol 2005 (Neoplasms) : Optimal induction of IFN-gamma by IL-12 occurred after an IV dose of 250 ng/kg
Réthi et al., Blood 2006 (Inflammation) : Similarly, transfection of DCs with SLAM strongly reduced CD40L induced IL-12 production ... Similarly, transfection of DCs with SLAM strongly reduced CD40L induced IL-12 production ... LPS induced IL-12 secretion, however, was not inhibited by SLAM engagement ... LPS induced IL-12 secretion, however, was not inhibited by SLAM engagement
Pruett et al., Toxicol Appl Pharmacol 2006 : NAC exacerbated the SMD induced suppression of IL-12 and the SMD induced enhancement of IL-10 in the serum
Maecker et al., Cell 2005 (Hypersensitivity) : Thus, TWEAK suppresses production of IFN-gamma and IL-12 , curtailing the innate response and its transition to adaptive TH1 immunity
Fronhofer et al., J Leukoc Biol 2006 : Role of PKC isoforms in the Fc ( gamma ) R-mediated inhibition of LPS stimulated IL-12 secretion by macrophages ... Ligation of Fc receptors for immunoglobulin G ( FcgammaRs ) inhibits lipopolysaccharide (LPS) stimulated secretion of interleukin (IL)-12 by macrophages ... Similarly, activation of PKC with phorbol 12-myristate 13-acetate ( PMA ) depressed LPS stimulated IL-12p40 secretion, and depletion of PKC augmented LPS stimulated IL-12p40 secretion ... Antisense down-regulation of PKC-delta increased LPS stimulated IL-12p40 secretion and fully prevented the effects of FcgammaR ligation or PMA on IL-12p40 secretion ... In contrast, down-regulation of PKC-epsilon blocked LPS stimulated secretion of IL-12p40 ... Down-regulation of PKC-alpha had no effect on LPS stimulated IL-12p40 secretion ... Down-regulation of PKC-alpha had no effect on LPS stimulated IL-12p40 secretion ... The results suggest a negative role for PKC-delta and a positive role for PKC-epsilon in the regulation of LPS stimulated IL-12p40 secretion
Arena et al., Int Immunopharmacol 2006 : EPS-1 induced IL-12 , IFN-gamma, IFN-alpha, TNF-alpha and IL-18, but not IL-4
Curti et al., Exp Hematol 2005 (Leukemia, Myeloid) : Mature AML-DCs stimulated naive and, to a lesser extent, leukemic cell ( LC ) -cultured T cells more efficiently than their immature counterparts and their activity was mediated by IL-12
Józefowski et al., J Immunol 2005 : Conversely, SR-AI/II-deficient PEMs produced 2.4 and 1.8 times more IL-12 than wild-type PEMs in response to LPS or LPS and IFN-gamma , respectively ... Conversely, SR-AI/II-deficient PEMs produced 2.4 and 1.8 times more IL-12 than wild-type PEMs in response to LPS or LPS and IFN-gamma, respectively
Rezende et al., Int Endod J 2005 : The cellular viability and the production of tumour necrosis factor-alpha, interleukin (IL)-12 and IL-10 in response to stimulation with interferon-gamma and Fusobacterium nucleatum or Peptostreptococcus anaerobius were evaluated
Okamoto et al., Chest 2005 (Pleural Effusion, Malignant...) : Although malignant pleural effusions showed low levels of Th1 and Th2 cytokines and ratios of IFN-gamma and IL-12 to IL-4 were low, isolated T-cells produced a significant level of IFN-gamma in the presence of IL-12 and IL-18
Walker et al., Arch Virol Suppl 2005 (Ehrlichiosis) : Ehrlichiae attach to L-selectin and E-selectin, inhibit phagolysosomal fusion, apoptosis, and JAK/STAT activation, and downregulate IL-12 , IL-15, IL-18, TLR2 and 3, and CD14 ... Ehrlichiae attach to L-selectin and E-selectin, inhibit phagolysosomal fusion, apoptosis, and JAK/STAT activation, and downregulate IL-12 , IL-15, IL-18, TLR2 and 3, and CD14
Kim et al., J Immunol 2006 : The novel cytokine p43 induces IL-12 production in macrophages via NF-kappaB activation, leading to enhanced IFN-gamma production in CD4+ T cells ... In this study, we have attempted to characterize the effects of p43 on the generation of IL-12 in mouse macrophages ... The stimulatory effect of p43 on the activation of IL-12p40 promoter was mapped to a region harboring an NF-kappaB binding site ... In addition, the p43 stimulated IL-12 induction and NF-kappaB DNA binding activity were significantly suppressed by caffeic acid phenethyl ester and BAY11-7082, both inhibitors of NF-kappaB activation, indicating that p43 induced the production of IL-12 in macrophages mainly via the activation of NF-kappaB
Middleton et al., J Immunol 2006 : Furthermore, LPS stimulation triggered an increase in wild-type macrophage 12/15-LO activity, whereas pharmacological inhibition of 12/15-LO activity suppressed LPS induced IL-12 production in wild-type macrophages ... 12/15-LO-deficient macrophages also produced reduced levels of IL-12 in response to TLR2 stimulation, but not in response to CpG ( TLR9 ) or CD40/CD40L mediated activation ... 12/15-LO-deficient macrophages also produced reduced levels of IL-12 in response to TLR2 stimulation, but not in response to CpG ( TLR9 ) or CD40/CD40L mediated activation ... 12/15-LO-deficient macrophages also produced reduced levels of IL-12 in response to TLR2 stimulation, but not in response to CpG ( TLR9 ) or CD40/CD40L mediated activation ... 12/15-LO-deficient macrophages also produced reduced levels of IL-12 in response to TLR2 stimulation, but not in response to CpG ( TLR9 ) or CD40/CD40L mediated activation
Yang et al., Clin Exp Immunol 2006 (MAP Kinase Signaling System...) : The enhancement of IL-12 levels by inhibition of PI 3-K and ERK 1/2 was not reversed by neutralization of TNF-alpha or addition of rhTNF-alpha, suggesting that the negative regulation of IL-12 is not mediated by concomitant TNF-alpha suppression ... The enhancement of IL-12 levels by inhibition of PI 3-K and ERK 1/2 was not reversed by neutralization of TNF-alpha or addition of rhTNF-alpha, suggesting that the negative regulation of IL-12 is not mediated by concomitant TNF-alpha suppression ... The enhancement of IL-12 levels by inhibition of PI 3-K and ERK 1/2 was not reversed by neutralization of TNF-alpha or addition of rhTNF-alpha, suggesting that the negative regulation of IL-12 is not mediated by concomitant TNF-alpha suppression ... The enhancement of IL-12 levels by inhibition of PI 3-K and ERK 1/2 was not reversed by neutralization of TNF-alpha or addition of rhTNF-alpha, suggesting that the negative regulation of IL-12 is not mediated by concomitant TNF-alpha suppression
Masunaga et al., Transplantation 2005 : In contrast, although monomeric soluble CD40/Myc/His suppressed IL-12 production in a similar manner to that achieved by CD40Ig, it did not augment IL-2 production ... In contrast, although monomeric soluble CD40/Myc/His suppressed IL-12 production in a similar manner to that achieved by CD40Ig, it did not augment IL-2 production
Kao et al., Regul Pept 2006 (Gastritis) : Pretreatment of bone marrow derived dendritic cells with somatostatin results in decreased IL-12 production, and lower splenocyte proliferation induced by H. pylori stimulated dendritic cells
Motegi et al., J Leukoc Biol 2006 (Shock, Septic...) : A lethal human septic shock model, mouse generalized Shwartzman reaction (GSR), was elicited by two consecutive lippolysaccharide (LPS) injections ( 24 h apart ) in which interferon-gamma (IFN-gamma) induced by interleukin (IL)-12 played a critical role in the priming phase, and tumor necrosis factor (TNF) was an important effector molecule in the second phase ... IL-12 induced IFN-gamma up-regulated Toll-like receptor 4 (TLR-4) on macrophages of adults
Park et al., Exp Mol Med 2005 : In addition, RANKL increased the expression of TNFalpha, MCP-1, and IL-6 but not of IL-10, IL-12 , IFN-gamma, and iNOS
Kozma et al., J Immunol 2006 : PIBF activated suppressor of cytokine signaling-3 and inhibited IL-12 induced suppressor of cytokine signaling-1 activation
Tomczak et al., J Immunol 2006 (MAP Kinase Signaling System) : Instead, it was linked to the induction of c-Fos , a known inhibitor of IL-12 p40 expression
Yamauchi et al., Nihon Rinsho Meneki Gakkai Kaishi 2005 : Gal-9 induced phosphorylation of the p38 MAPK and ERK1/2 in DCs, and an inhibitor of p38 signaling, but not inhibitors of signaling by either ERK1/2 or phosphatidylinositol 3-kinase, blocked Gal-9 induced up-regulation of costimulatory molecule expression and IL-12 production
Davis et al., Particle and fibre toxicology 2006 : We hypothesized that IL-12 might stimulate lymphocyte activation and the up-regulation of IFN-gamma , and consequently be an essential mediator for silicosis
Amcheslavsky et al., J Cell Physiol 2006 : In conclusion, activation of TLR9 by its ligand, CpG-ODN, results in synthesis and release of IL-12 opposing RANKL induced osteoclast differentiation
Hara et al., Int Immunol 2006 : IL-12 enhances the expression of CXCR6 in anti-CD3/CD28 stimulated CD8+ T cells and their adhesion to the BLS4 cell surface in a TNFalpha dependent fashion
Tripathi et al., Clin Exp Immunol 2006 (Leishmaniasis, Visceral) : Among 21 responders to WE, frequent proliferative responses were seen to fractions F1-F4 ( MW > 64.2 kDa ) and none to fractions F5-F11 ; fractions F1-F11 stimulated comparable levels of IFN-gamma and IL-12 while IL-10 levels were higher in response to F5-F11 compared to F1-F4
Zhou et al., Exp Mol Pathol 2006 : Interestingly, in contrast to mature DCs derived from TNF-alpha induced immature DC cultures without PsL-EGFmAb, immature DCs treated with PsL-EGFmAb for 7 days were completely blocked their maturation, as evidenced by decreased expression of costimulatory molecules CD80, CD86, and CD83, inhibited production of IL-12 , and inability to activate naïve T cells in vitro
Kuwata et al., Immunity 2006 (Colitis...) : IkappaBNS-deficient macrophages and dendritic cells show increased TLR mediated expression of genes such as IL-6 and IL-12p40 , which are induced late after TLR stimulation
Woelbing et al., J Exp Med 2006 (Leishmaniasis, Cutaneous) : Uptake of Leishmania major by dendritic cells (DCs) results in activation and interleukin (IL)-12 release
Muthian et al., J Clin Immunol 2006 (Demyelinating Diseases...) : Further analyses showed that the COX-2 inhibitors reduced neural antigen induced IL-12 production, T cell proliferation and Th1 differentiation ex vivo and in vitro
El Bassam et al., Immunology 2006 (Asthma...) : To evaluate whether the modulatory effect of IL-16 on cytokine synthesis was mediated by interferon-gamma (IFN-gamma), IL-10, IL-12 or IL-18, allergen stimulated PBMC were cultured in presence of IL-16 and neutralizing concentrations of relevant antibodies
Maroof et al., Immunology 2006 : The presence of interleukin-4 (IL-4) during the generation of dendritic cells ( DC ) from precursor cells results in measurable increases of IL-12 in supernatants but IL-4 secretion has not been reported
Liu et al., Int Immunopharmacol 2006 : Histamine did not affect IL-12 induced phosphorylation of STAT4
Bachmann et al., Cell Microbiol 2006 : Flagellin synergized with IL-12 for production of IFN-gamma and augmented secretion of interferon-inducible protein-10, a CXC-chemokine that is key to the generation of Th1-type responses
Lu et al., Eur J Pharmacol 2006 : These results suggest that THC induced suppression of serum IL-12 is partly due to a suppression of IL-12 production by dendritic cells and that G ( i ) signaling and cannabinoid receptors, but not TRPV1 , are involved in this suppressive effect ... These results suggest that THC induced suppression of serum IL-12 is partly due to a suppression of IL-12 production by dendritic cells and that G ( i ) signaling and cannabinoid receptors, but not TRPV1, are involved in this suppressive effect
Davey et al., Tissue Antigens 2006 : In addition, SOCS1 has a complex role in T cell activation, and studies have revealed significant roles for SOCS1 in the regulation of IL-4, IL-12 and IL-15 in vivo
Katsuno et al., Clin Immunol 2006 : Ciprofloxacin ( CIP ), which is useful for the clinical treatment of infections due to its antibacterial properties after transplantation, was shown to suppress the IFN-gamma and IL-12 production, the lymphocyte proliferation and the ICAM-1, B7.1, B7.2 and CD40 expression on monocytes during MLR in the presence of IL-18
Kramer et al., J Leukoc Biol 2006 (Crohn Disease) : Moreover, they lack MDP induced enhancement of TLR mediated tumor necrosis factor alpha, interleukin (IL)-12 , and IL-10 production, which is observed in control DC with intact NOD2
Molenda et al., Mol Immunol 2006 : In keratinocytes, IL-12 activates STAT3 and STAT4 ; surprisingly, despite activating these transcription factors, the transcriptional effects of IL-12 did not rise above background levels ... In keratinocytes, IL-12 activates STAT3 and STAT4 ; surprisingly, despite activating these transcription factors, the transcriptional effects of IL-12 did not rise above background levels
Alloza et al., Mol Pharmacol 2006 : Analysis of intracellular fractions revealed that both CE and CEA increased the association of IL-12 with calreticulin , an endoplasmic reticulum-resident chaperone involved in the retention of misfolded cargo proteins while blocking interaction with ERp44 ... Analysis of intracellular fractions revealed that both CE and CEA increased the association of IL-12 with calreticulin, an endoplasmic reticulum-resident chaperone involved in the retention of misfolded cargo proteins while blocking interaction with ERp44
Obata et al., Scand J Immunol 2006 : This was confirmed by mRNA analysis, which showed that H ( 2 ) O ( 2 ) increased LPS induced mRNA expression of both IL-12p40 and IL-12p35 subunits with an increased p40/p35 ratio
Hoeve et al., Eur J Immunol 2006 (Arthritis...) : Divergent effects of IL-12 and IL-23 on the production of IL-17 by human T cells ... In contrast, IL-12 mediated specific inhibition of IL-17 production
Owaki et al., J Immunol 2006 : Although IL-27 enhanced IFN-gamma production from naive CD4+ T cells stimulated with plate coated anti-CD3 and anti-CD28 in the presence of IL-12 , IL-27 simultaneously inhibited CD28 mediated IL-2 production
Mpiga et al., Scand J Immunol 2006 (Chlamydia Infections) : When infected HeLa cells were co-cultured with THP-1 cells, IL-6 and IL-8 secretion was sustained, IL-1beta expression followed a bell shaped curve and IL-10, IL-12p70 and TNF-alpha synthesis was down regulated ... When infected HeLa cells were co-cultured with THP-1 cells, IL-6 and IL-8 secretion was sustained, IL-1beta expression followed a bell shaped curve and IL-10, IL-12p70 and TNF-alpha synthesis was down regulated
Pedraza-Sánchez et al., Int Immunopharmacol 2006 : The early activation marker CD69 was induced at 4 h in NK cells > B cells > T cells > monocytes whereas at 24 h CD80 and CD86 levels were augmented on monocytes and IL-12 was induced ; HLA-DR levels increased on both B cells and monocytes ... The early activation marker CD69 was induced at 4 h in NK cells > B cells > T cells > monocytes whereas at 24 h CD80 and CD86 levels were augmented on monocytes and IL-12 was induced ; HLA-DR levels increased on both B cells and monocytes
Kupfahl et al., Int Immunopharmacol 2006 (Listeriosis) : In summary these results show that lentinan enhances the protective CD8 T-cell response against L. monocytogenes probably by a mechanism that involves the IL-12 mediated augmentation of the specific antilisterial CD8 T-cell response
Tortorella et al., J Gerontol A Biol Sci Med Sci 2006 : Interestingly, no difference in PHA triggered signal transducer and activator of transcription 4 ( STAT4 ) phosphorylation between young and elderly donors was found, and a significant IL-12 induced STAT4 activation occurred only in PHA preactivated cells from the younger group
Yanagihori et al., J Invest Dermatol 2006 (Behcet Syndrome...) : As assessed by semiquantitative reverse transcription-PCR and ELISA, stimulation with Streptococcal antigens specifically increased expression of IL-12 p40 mRNA and protein, in conjunction with IL-12 p70 induction , in peripheral blood mononuclear cells from heterozygous patients
Peng et al., Immunol Cell Biol 2006 (Autoimmune Diseases...) : IL10 and IL12B polymorphisms each influence IL-12p70 secretion by dendritic cells in response to LPS ... In contrast, IL-10 , which is also produced by DC, negatively regulates IL-12 production ... Here we show that polymorphisms not only of IL12B, but also in the IL10 promoter, influence IL-12p70 secretion by monocyte derived DC in response to LPS
Zeuthen et al., Clinical and vaccine immunology : CVI 2006 : Interestingly, we found that when weakly IL-12- and TNF-alpha inducing LAB and strong IL-12- and TNF-alpha inducing LAB were mixed, the weakly IL-12- and TNF-alpha inducing LAB efficiently inhibited otherwise strong IL-12- and TNF-alpha inducing LAB, yet when weakly IL-12- and TNF-alpha inducing LAB were mixed with G- bacteria, they synergistically induced IL-12 and TNF-alpha
An et al., J Ethnopharmacol 2006 : However, GH increased IFN-gamma/LPS induced IL-12p40 production ... However, GH increased IFN-gamma/LPS induced IL-12p40 production
Yeboah-Manu et al., J Leukoc Biol 2006 (Mycobacterium Infections, Nontuberculous...) : Interleukin (IL)-12 secretion by PPD stimulated PBMC was not reduced in BU patients, indicating that reduction in IFN-gamma responses was not caused by diminished IL-12 production
Scarponi et al., J Interferon Cytokine Res 2006 (Dermatitis, Atopic...) : In monocytes and DCs, IFN-kappa induces tumor necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10) and inhibits lipopolysaccharide (LPS) induced IL-12 ... In monocytes and DCs, IFN-kappa induces tumor necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10) and inhibits lipopolysaccharide (LPS) induced IL-12
Muthian et al., J Neurosci Res 2006 (Disease Models, Animal...) : Treatment of activated T cells with 1,25 ( OH ) 2D3 also inhibited the IL-12 induced tyrosine phosphorylation of JAK2, TYK2, STAT3 , and STAT4 in association with a decrease in T cell proliferation in vitro ... Treatment of activated T cells with 1,25 ( OH ) 2D3 also inhibited the IL-12 induced tyrosine phosphorylation of JAK2, TYK2, STAT3 , and STAT4 in association with a decrease in T cell proliferation in vitro ... Treatment of activated T cells with 1,25 ( OH ) 2D3 also inhibited the IL-12 induced tyrosine phosphorylation of JAK2, TYK2, STAT3, and STAT4 in association with a decrease in T cell proliferation in vitro ... Treatment of activated T cells with 1,25 ( OH ) 2D3 also inhibited the IL-12 induced tyrosine phosphorylation of JAK2, TYK2 , STAT3, and STAT4 in association with a decrease in T cell proliferation in vitro ... Treatment of activated T cells with 1,25 ( OH ) 2D3 also inhibited the IL-12 induced tyrosine phosphorylation of JAK2 , TYK2, STAT3, and STAT4 in association with a decrease in T cell proliferation in vitro
Shan et al., Cell Mol Immunol 2006 (Carcinoma...) : IL-23 also promoted the production of Th1 cytokines such as IFN-gamma, IL-12 and TNF-alpha
Strengell et al., J Leukoc Biol 2006 : IL-21 pretreatment also dramatically reduced LPS stimulated production of tumor necrosis factor alpha, IL-12 , CC chemokine ligand 5 (CCL5), and CXC chemokine ligand 10 (CXCL10) but not that of CXCL8
Pammit et al., Infect Immun 2006 (Pneumonia, Bacterial...) : Animals primed with the F. novicida DeltaiglC ( KKF24 ) mutant induced robust splenic gamma interferon ( IFN-gamma ) and interleukin-12 (IL-12) recall responses with negligible IL-4 production as well as the production of antigen-specific serum immunoglobulin G1 ( IgG1 ) and IgG2a antibodies
Yanagawa et al., Immunology 2006 : Taken together, we concluded that IL-6 and IL-12 productions in response to CD40 ligation were controlled by p38 MAPK and NIK mediated alternative pathway, respectively, in mature DCs ... Taken together, we concluded that IL-6 and IL-12 productions in response to CD40 ligation were controlled by p38 MAPK and NIK mediated alternative pathway, respectively, in mature DCs
Reeve et al., Photochem Photobiol 2006 (Dermatitis, Contact...) : UVA enhanced radiation also upregulated the expression of cutaneous IFN-gamma and IL-12 and inhibited expression of both IL-6 and IL-10 , compared with the activity of SSUV ... UVA enhanced radiation also upregulated the expression of cutaneous IFN-gamma and IL-12 and inhibited expression of both IL-6 and IL-10, compared with the activity of SSUV
Hu et al., Infect Immun 2006 : In addition, Campylobacter infected DCs triggered activation of NF-kappaB and significantly stimulated production of interleukin-1beta (IL-1beta), IL-6, IL-8, IL-10, IL-12 , gamma interferon, and tumor necrosis factor alpha (TNF-alpha) compared to uninfected DCs
Spensieri et al., Infect Immun 2006 : ACT- 18HS19 infection induced the expression of the transcription factors interferon regulatory factor 1 (IRF-1) and IRF-8 and of beta interferon, involved in IL-12 p35 regulation , and the expression of these genes was inhibited by D-cAMP addition and in WT18323 infected MDDC
Pesu et al., Blood 2006 : Interleukin 12 (IL-12) is a major inducer of interferon gamma (IFN-gamma) and the principal mediator of T helper 1 ( Th1 ) differentiation ... Thus, we conclude that IL-12 induction of furin might represent a new aspect of IFN-gamma regulation and control of Th1 differentiation
Zhu et al., Int Immunol 2006 (MAP Kinase Signaling System) : In addition, anti-IL-10 neutralizing antibody plus indomethacin could abrogate the inhibitory effects of endogenous IL-10 and prostaglandin E2 on the production of IL-12 by resident pMphi possibly through suppressing ERK activation
Wittmann et al., J Leukoc Biol 2006 : Critical involvement of IL-12 in IFN-gamma induction by calcineurin antagonists in activated human lymphocytes ... This increased IFN-gamma production is dependent on T cell receptor ( TCR ) and CD28 signaling as well as on the presence of IL-12
Boysen et al., BMC immunology 2006 : A higher proportion of CD2- NK cells had intracellular interferon-gamma in the cytoplasm in response to IL-2 and IL-12 stimulation, and the CD2- subset secreted more interferon-gamma when cultured separately
Helguera et al., Mol Cancer Ther 2006 (Colonic Neoplasms...) : However, both bi-AbFPs retained the capacity to stimulate IL-12 dependent IFN-gamma secretion in the NK cell line KY-1, and IL-12/IL-2 bi-AbFP induced NK activity in splenocytes
Fahey et al., J Leukoc Biol 2006 : IFN-beta increases the expression of the anti-inflammatory cytokine IL-10 and suppresses IL-12 ... Our aim was to investigate the effects of dexamethasone on STAT4 activation by IFN-beta and IL-12 in human T cell blasts ... We report that dexamethasone decreases IL-12 induced STAT4 phosphorylation and IFN-gamma production and enhances IFN-beta induced STAT4 activation and IL-10 production ... We report that dexamethasone decreases IL-12 induced STAT4 phosphorylation and IFN-gamma production and enhances IFN-beta induced STAT4 activation and IL-10 production
Agrawal et al., J Immunol 2006 (Encephalomyelitis, Autoimmune, Experimental...) : Consistent with this observation, dendritic cells from ERK1 ( -/- ) mice show enhanced IL-12p70 and reduced IL-10 secretion in response to TLR stimulation
Kuo et al., J Immunol 2006 : Our results reveal that class III PI3K specifically regulates CpG oligodeoxynucleotide ( ODN ) -induced cytokine and NO production as well as NF-kappaB activation, whereas class I PI3K regulates both CpG ODN- and LPS induced IL-12 production and NF-kappaB activation ... Our results reveal that class III PI3K specifically regulates CpG oligodeoxynucleotide ( ODN ) -induced cytokine and NO production as well as NF-kappaB activation, whereas class I PI3K regulates both CpG ODN- and LPS induced IL-12 production and NF-kappaB activation
Inoue et al., Arerugi 2006 (Asthma...) : SOCS3 controls IL-12 dependent STAT4 activation and Th2 differentiation process ... SOCS3 controls IL-12 dependent STAT4 activation and Th2 differentiation process
Hao et al., Cancer Immunol Immunother 2006 (Neoplasms) : IL-23 can enhance the proliferation of memory T cells and the production of IFN-gamma, IL-12 and TNF-alpha from activated T cells
Polanczyk et al., J Neurosci Res 2006 : We found that E2 treatment in vivo strongly reduced recovery of APC from the peritoneal cavity and inhibited induction of the inflammatory cytokines interleukin (IL)-12 and interferon-gamma but enhanced secretion of IL-10
Kitanaka et al., Biochem Biophys Res Commun 2006 : Collectively, it is suggested that E-FABP may be a novel negative regulator of IL-12 production in DCs, and this regulation may be exerted via its involvement in the p38MAPK mediated transcription of IL-12p35 ... Collectively, it is suggested that E-FABP may be a novel negative regulator of IL-12 production in DCs, and this regulation may be exerted via its involvement in the p38MAPK mediated transcription of IL-12p35
Monzavi-Karbassi et al., Int J Mol Med 2006 (Sarcoma) : Examining tumor challenged animals, we observed that peptide immunization and not tumor cells rendered IL-12 responsiveness to T-cells, as T-cells from peptide immunized mice produced IFN-gamma upon stimulation with IL-12
Tso et al., Lupus 2006 (Insulin Resistance...) : IL-12 has a synergistic effect with IL-18, and both cytokines are inducers of interferon (IFN)-gamma
Harada et al., J Interferon Cytokine Res 2006 : We recently found that the splenocytes from naive DBA/1 and DBA/2 mice are potently induced by SCG to produce interferon- gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha), granulocyte-macrophage colony stimulating factor ( GM-CSF ), and interleukin-12p70 (IL-12p70) , and that GM-CSF plays a key biologic role among these cytokines ... Cell-cell contact involving intercellular adhesion molecule-1 ( ICAM-1 ) and lymphocyte function associated antigen-1 ( LFA-1 ) was an essential step for the induction of GM-CSF and IFN-gamma by SCG but not for the induction of TNF-alpha or IL-12p70 by SCG ... SCG directly induced adherent splenocytes to produce TNF-alpha and IL-12p70
Oishi et al., Cytokine 2006 : The rhG-CSF induced a pre-PHx downregulation of the IL-12p70 protein levels, while also promoting the post-PHx reduction of the protein levels of IL-12p70 and IFN-gamma ... The rhG-CSF induced a pre-PHx downregulation of the IL-12p70 protein levels, while also promoting the post-PHx reduction of the protein levels of IL-12p70 and IFN-gamma ... The rhG-CSF induced a pre-PHx downregulation of the IL-12p70 protein levels, while also promoting the post-PHx reduction of the protein levels of IL-12p70 and IFN-gamma
Muthana et al., Immunobiology 2006 : Evidence to date suggests that IL-12 and IL-10 secretion is reciprocally regulated and that IL-10 inhibits IL-12 secretion ... Neutralization of IL-10 increased the secretion of IL-12 ( p40 ) by resting BMDCs, but decreased IL-12 ( p40 ) secretion by LPS activated BMDCs. Pre-incubation of resting BMDCs for 24h with neutralizing antibody to IL-10 reduced the subsequent secretion of IL-10 in allogeneic cultures of Lewis CD3 ( + ) T cells with resting and LPS activated Wistar BMDCs, and enhanced IL-12 ( p40 ) secretion in allogeneic cultures with LPS activated BMDCs. IL-10 neutralization had no effect on the levels of IL-12 ( p70 ), IFN-gamma or IL-4 in allogeneic cultures
Rajagopalan et al., Shock 2006 (Inflammation...) : Defective IL-12 or IL-4 receptor signaling significantly decreased or increased serum IFN-gamma , respectively
Gorski et al., Int Immunol 2006 : IL-18 and IL-12p70 were primarily required for induction of IFN-gamma by both synthetic and natural TLR-8 ligands, while type I IFN was required for induction of CD69 on NK cells by the TLR-7 agonist 3M-001
Mohammad et al., Int Immunol 2006 (Diabetes Mellitus, Experimental) : Dysregulation of TLR4 expression in the diabetic state correlated with increased nuclear factor kappa B (NF-kappaB) activation in response to the TLR4 ligand LPS and higher expression of IL-12p40 , tumor necrosis factor alpha (TNFalpha), IL-6 and inducible nitric oxide synthase but lowered expression of IL-10
Davoine et al., Int Arch Allergy Immunol 2006 : Effects of IL- 12 on purified human blood eosinophils were evaluated for peroxidase (EPO) release, eotaxin induced migration through a model of basement membrane ( Matrigel ), and survival ... IL-12 inhibited 50 % of PAF and secretory IgA induced EPO release ( n = 8, p < 0.001 ) ... IL-12 inhibited 50 % of PAF and secretory IgA induced EPO release ( n = 8, p < 0.001 )
Zhu et al., J Pharmacol Exp Ther 2006 (Encephalomyelitis, Autoimmune, Experimental) : In vitro study also showed PSE inhibited interferon (IFN)-gamma dependent CXCR3 expression in T cells through suppressing T-cell receptor ( TCR ) ligation induced IFN-gamma production, whereas it inhibited interleukin (IL)-12 dependent CCR5 expression through suppressing IL-12 reactivity in TCR triggered T cells
Nembrini et al., J Immunol 2006 : Upon strong signaling through the TCR, IL-12 , IL-18, and IL-23 play negligible roles in the induction of IFN-gamma , whereas autocrine IFN-gamma is an important component in sustaining its own secretion
Shaw et al., J Immunol 2006 (Genetic Predisposition to Disease...) : The Jak, Tyk2, is activated in response to IL-12 and IFN-alphabeta and promotes IFN-gamma production by Th1-type CD4 cells ... The Jak , Tyk2, is activated in response to IL-12 and IFN-alphabeta and promotes IFN-gamma production by Th1-type CD4 cells
Blachère et al., J Immunol 2006 : Concerning the interaction between CD4 ( + ) T cells and DCs, we tested the hypothesis that CD40 engagement on DCs is critical for IL-12p70 ( IL-12 ) production and subsequent stimulation of IFN-gamma release by CD8 ( + ) T cells ... Concerning the interaction between CD4 ( + ) T cells and DCs, we tested the hypothesis that CD40 engagement on DCs is critical for IL-12p70 ( IL-12 ) production and subsequent stimulation of IFN-gamma release by CD8 ( + ) T cells
Bekeredjian-Ding et al., J Immunol 2006 : T cell independent, TLR induced IL-12p70 production in primary human monocytes ... Simultaneous stimulation of TLR4 and TLR8 induced IL-12p70 in primary human monocytes ... Simultaneous stimulation of TLR4 and TLR8 induced IL-12p70 in primary human monocytes ... IL-12p70 production in peripheral blood myeloid dendritic cells required combined stimulation of TLR7/8 ligands together with TLR4 or with TLR3 ligands ... In the presence of T cell derived IL-4, but not IFN-gamma, stimulation with TLR7/8 ligands was sufficient to stimulate IL-12p70 production ... In the presence of T cell derived IL-4, but not IFN-gamma , stimulation with TLR7/8 ligands was sufficient to stimulate IL-12p70 production ... In monocytes, type I IFN was required but not sufficient to costimulate IL-12p70 induction by TLR8 ligation
Kriegel et al., Immunology 2006 : While IL-12 induces interferon-gamma (IFN-gamma) production and maturation of Th1 cells, IL-4 is thought to antagonize IL-12 and to favour Th2 development
Bhattacharya et al., EMBO J 2006 : IL-6 and IL-12 specifically regulate the expression of Rab5 and Rab7 via distinct signaling pathways ... IL-6 and IL-12 specifically regulate the expression of Rab5 and Rab7 via distinct signaling pathways
Qian et al., Blood 2006 : Here, we report that unlike immature DCs, diffDCs secrete more interleukin 10 (IL-10) but little IL-12p70 in response to lipopolysaccharide (LPS) or other TLR agonists
Wheeler et al., Immunology 2006 (Encephalomyelitis, Autoimmune, Experimental) : There was greater expression of IL-12/23p40 by antigen presenting cells in these mice, and in vitro, TNFR1-/- antigen presenting cells induced greater secretion of IFN-gamma but not interleukin (IL)-17 when cultured with primed T cells than did WT antigen presenting cells ... There was greater expression of IL-12/23p40 by antigen presenting cells in these mice, and in vitro, TNFR1-/- antigen presenting cells induced greater secretion of IFN-gamma but not interleukin (IL)-17 when cultured with primed T cells than did WT antigen presenting cells
Yoon et al., Mol Pharmacol 2006 : In the presence of apigenin, CD80 , CD86, and major histocompatibility complex class I and II molecules, expressions on DC were significantly suppressed, and lipopolysaccharide (LPS) induced interleukin (IL)-12 expression was impaired ... In the presence of apigenin, CD80, CD86 , and major histocompatibility complex class I and II molecules, expressions on DC were significantly suppressed, and lipopolysaccharide (LPS) induced interleukin (IL)-12 expression was impaired ... In the presence of apigenin, CD80, CD86, and major histocompatibility complex class I and II molecules, expressions on DC were significantly suppressed, and lipopolysaccharide (LPS) induced interleukin (IL)-12 expression was impaired
Liu et al., J Immunol 2006 (Acute Disease...) : Diphtheria toxin ( DT ) injection causes transient DC depletion in a transgenic mouse expressing Simian DT receptors under the control of the CD11c promoter, allowing us to investigate the role of DCs in IL-12 production
Suneetha et al., J Med Virol 2006 (Genetic Predisposition to Disease...) : IL-12 induces production of IFN-gamma , a potent antiviral agent
Wei et al., Biochimie 2006 : Promutoxin is also able to stimulate the release of IL-12 , TNFalpha, IL-6 and IL-1beta from human monocytes, and induce IL-2, TNFalpha and IL-6 release from T cells, indicating that this snake venom group IIA PLA(2) is actively involved in the inflammatory process in man caused by snake venom poisoning ... Promutoxin is also able to stimulate the release of IL-12 , TNFalpha, IL-6 and IL-1beta from human monocytes, and induce IL-2 , TNFalpha and IL-6 release from T cells, indicating that this snake venom group IIA PLA(2) is actively involved in the inflammatory process in man caused by snake venom poisoning ... Promutoxin is also able to stimulate the release of IL-12 , TNFalpha, IL-6 and IL-1beta from human monocytes, and induce IL-2, TNFalpha and IL-6 release from T cells, indicating that this snake venom group IIA PLA(2) is actively involved in the inflammatory process in man caused by snake venom poisoning
Franke et al., Ann Thorac Surg 2006 (Disease Susceptibility...) : To better understand these processes, we assessed the role of IL-12 in IFN-gamma synthesis in vitro before and after cardiac surgery ... These findings suggest that postoperative suppression of IFN-gamma release is caused by a decrease in IL-12 synthesis
Khader et al., J Exp Med 2006 (Tuberculosis, Pulmonary) : These data define a novel and fundamental role for IL-12p40 in the pathogen induced activation of pulmonary DCs
Filipe-Santos et al., J Exp Med 2006 (Genetic Predisposition to Disease...) : X-linked susceptibility to mycobacteria is caused by mutations in NEMO impairing CD40 dependent IL-12 production ... IL-12 production was also impaired as the result of a specific defect in NEMO- and NF-kappaB/c-Rel mediated CD40 signaling after the stimulation of monocytes and dendritic cells by CD40L expressing T cells and fibroblasts, respectively ... IL-12 production was also impaired as the result of a specific defect in NEMO- and NF-kappaB/c-Rel mediated CD40 signaling after the stimulation of monocytes and dendritic cells by CD40L expressing T cells and fibroblasts, respectively ... IL-12 production was also impaired as the result of a specific defect in NEMO- and NF-kappaB/c-Rel mediated CD40 signaling after the stimulation of monocytes and dendritic cells by CD40L expressing T cells and fibroblasts, respectively ... IL-12 production was also impaired as the result of a specific defect in NEMO- and NF-kappaB/c-Rel mediated CD40 signaling after the stimulation of monocytes and dendritic cells by CD40L expressing T cells and fibroblasts, respectively ... They also demonstrate the importance of the T cell- and CD40L triggered, CD40-, and NEMO/NF-kappaB/c-Rel mediated induction of IL-12 by monocyte derived cells for protective immunity to mycobacteria in humans ... They also demonstrate the importance of the T cell- and CD40L triggered, CD40-, and NEMO/NF-kappaB/c-Rel mediated induction of IL-12 by monocyte derived cells for protective immunity to mycobacteria in humans ... They also demonstrate the importance of the T cell- and CD40L triggered, CD40-, and NEMO/NF-kappaB/c-Rel mediated induction of IL-12 by monocyte derived cells for protective immunity to mycobacteria in humans
Acevedo et al., Vaccine 2006 : AGs also induced the production of IL-12 and nitric oxide ( NO ) in the U937 human histiocyte and J774 mouse macrophage cell lines, respectively
Saegusa et al., Mol Immunol 2007 : Phosphatidylinositol 3-kinase mediated regulation of IL-10 and IL-12 production in macrophages stimulated with CpG oligodeoxynucleotide ... The aim of this study was to clarify the role of phosphatidylinositol 3-kinase (PI3K) on the production of interleukine-10 (IL-10) and IL-12 in mouse peritoneal macrophages stimulated with CpG-ODN ... The enhancement effect of PI3K inhibitors on IL-12 p70 production almost completely disappeared by the treatment with anti-IL-10 mAb ... These results suggest that PI3K positively and negatively regulates the production of CpG-ODN induced IL-10 and IL-12 p70, respectively, and negatively regulates IL-12 p70 production in macrophages through ERK mediated IL-10 induction
Husted et al., Inflamm Res 2006 (Reperfusion Injury) : Finally, isolated Kupffer cells produced TNFalpha in response to IL-23, but not IL-12 , suggesting that IL-23 may be the relevant initiator of the hepatic inflammatory response to ischemia/reperfusion
Ueda et al., Cancer Immunol Immunother 2007 (Neoplasms) : Although G-CSF mobilized monocytes (G/Mo) less effectively produced Th-1-type cytokines than control monocytes ( C/Mo ), DCs generated from G/Mo restored the same level of IL-12 production as that seen in DCs generated from C/Mo. T cell induction assay using recall antigen peptide and phenotypic analyses also demonstrated that DCs generated from G/Mo retained characteristics identical to those generated from C/Mo
Di Renzo et al., Int J Immunopathol Pharmacol 2006 (Chronic Disease...) : IL12 production in response to LPS was restored
Groeneweg et al., J Lipid Res 2006 (Inflammation) : Also, LPS induced cytokine secretion of TNF, IL-6, and IL-12 was enhanced , whereas secretion of IL-10 was strongly reduced after oxLDL preincubation ... Also, LPS induced cytokine secretion of TNF, IL-6, and IL-12 was enhanced, whereas secretion of IL-10 was strongly reduced after oxLDL preincubation
Awasthi et al., Cell Immunol 2006 : The ability of fetal baboon BMDCs to phagocytose E. coli bioparticles was significantly lower and they secreted lower level of LPS stimulated IL-12 as compared to the BMDCs from adult baboon
Yang et al., Int Immunopharmacol 2006 : In addition, PRL increased the expressions of IL-6, IL-10, IL-12 and TNF-alpha in SDCs
Li et al., J Immunol 2006 (Encephalomyelitis, Autoimmune, Experimental) : Indeed, in the present study we found that bone marrow derived DCs transduced with SOCS-3 significantly inhibited IL-12 induced activation of Stat4 and IL-23 induced activation of Stat3 ... Indeed, in the present study we found that bone marrow derived DCs transduced with SOCS-3 significantly inhibited IL-12 induced activation of Stat4 and IL-23 induced activation of Stat3
Kitaura et al., Immunol Lett 2006 : We previously reported that the proinflammatory cytokine IL-12 induced apoptosis in TNF-alpha mediated osteoclastogenesis in mouse bone marrow culture through an interaction of Fas and Fas ligand (FasL) ... We previously reported that the proinflammatory cytokine IL-12 induced apoptosis in TNF-alpha mediated osteoclastogenesis in mouse bone marrow culture through an interaction of Fas and Fas ligand (FasL) ... Furthermore, IL-18 and IL-12 synergistically induced apoptosis of adherent bone marrow cells in the presence of TNF-alpha, and up-regulated FasL transcription in non-adherent cells
Mullen et al., Immunology 2006 (Disease Susceptibility...) : However, drug treatment was equally effective in both mouse strains and restored serum IL-12 and IFN-gamma levels, and IFN-gamma production by ConA stimulated splenocytes of IL-18-deficient mice, to levels equivalent to similarly treated wild type mice
Józefowski et al., J Leukoc Biol 2006 : The ability of MARCO to bind CpG-ODNs and conversely, to costimulate IL-12 and NO production upon specific ligation with immobilized mAb is consistent with MARCO being a signaling receptor for CpG-ODNs, costimulating TLR9 mediated NO and IL-12 production in macrophages
Li et al., Am J Chin Med 2006 : Morin reduced the production of IL-12 and TNF-alpha in BM-DCs, in response to LPS stimulation
Saito et al., Clinical and vaccine immunology : CVI 2006 : We investigated the regulation of LPS induced IL-12 production by mouse macrophages ... These results suggest that some negative regulatory mechanisms against LPS induced IL-12 p40 production are constitutively functioning in RAW264.7 cells but not in the other types of cells
Al-Attiyah et al., Clin Exp Immunol 2006 (Tuberculosis) : Among the cytokines studied, interleukin (IL)-10 and IL-12 were not detected at all, whereas low levels of interferon (IFN)-gamma after 96 h ( 0.4 IU/ml ) and tumour necrosis factor (TNF)-alpha after 48 ( 135 pg/ml ) and 96 h ( 47 pg/ml ) of culture were detected in the supernatants of whole blood infected with M. tuberculosis
Abe et al., Eur J Immunol 2006 : This effect may be explained by exposure of the DC to endotoxin, because LPS attenuated IL-12 production by CpG stimulated liver MDC, both in vitro and in vivo
Wang et al., Scand J Immunol 2006 : The increased expression of IL-7R was associated with an increased thymocyte proliferation in response to anti-CD3 + IL-7 or anti-CD3 + IL-12 + IL-7
Koo et al., BMC immunology 2006 (Melioidosis) : Although IL-12 is essential for the IFN-gamma response, BALB/c and C57BL/6 splenocytes made similar amounts of IL-12 after infection
von Meyenn et al., Immunobiology 2006 : However, IL-12 production in DCs appears not exclusively dependent on TLR2 and only in TLR2/4/9-deficient DCs BCG induced IL-12 is reduced to background levels
Sjölin et al., J Immunol 2006 (Herpesviridae Infections) : In contrast, in response to CpG, DAP12 mediated effects on both IL-12 and IFN-alphabeta production were intrinsic to the pDCs ... However, in response to MCMV, only IL-12 but not IFN-alphabeta production was affected by pDC-intrinsic DAP12 signaling
Hewitson et al., J Immunol 2006 (Helminthiasis) : This defect is probably due to impaired CD40 dependent IL-12 production in vivo, because administration of anti-CD40 Ab, or rIL-12, restored IFN-gamma production by sdLN cells from CD154-/- mice
Moreno et al., J Immunol 2006 (Sepsis) : IL-12 and IL-18 play an important role in neutrophil migration ... IL-12 acts through induction of IFN-gamma and stimulation of phagocytic and microbicidal activities of neutrophils, rather than neutrophil migration per se ... IL-12 acts through induction of IFN-gamma and stimulation of phagocytic and microbicidal activities of neutrophils, rather than neutrophil migration per se
Keller et al., Infect Immun 2006 (Anemia...) : Blockade of IL-10 and the addition of recombinant IL-10 to cultured PBMC from children with SMA confirmed that IL-10 was responsible for malaria induced suppression of IL-12 ... Taken together, these results demonstrate that PfHz induced up-regulation of IL-10 is responsible for the suppression of IL-12 during malaria
Rowland et al., Infect Immun 2006 (Glanders) : The effects of IL-18, IL-27, and IL-12 on stimulation of the rapid IFN-gamma production were investigated in vitro by analyzing IFN-gamma production in the presence of heat killed B. mallei ... IL-12 was essential for IFN-gamma production in vitro ; IL-18 was also involved in induction of IFN-gamma, but IL-27 was not required for IFN-gamma production in response to heat killed B. mallei ... IL-12 was essential for IFN-gamma production in vitro ; IL-18 was also involved in induction of IFN-gamma, but IL-27 was not required for IFN-gamma production in response to heat killed B. mallei ... IL-12 was essential for IFN-gamma production in vitro ; IL-18 was also involved in induction of IFN-gamma, but IL-27 was not required for IFN-gamma production in response to heat killed B. mallei
Guerrero et al., Mol Immunol 2007 : Restimulation of spleen cells from primed mice with the Cry1Aa induced the production of significant levels of IL-12p70 whereas with Cry1Aa8, IFN-gamma production was stimulated
Velayudham et al., J Hepatol 2006 (Granuloma...) : Selective priming through TLR9 but not TLR2 induced granulomas, elevated serum ALT, and sensitized C57BL6 mice to increased LPS induced serum IL-6, IL-12 and IFNgamma levels
Bafica et al., J Immunol 2006 (Chagas Disease) : Our results reveal that TLR2 and TLR9 cooperate in the control of parasite replication and that TLR9 has a primary role in the MyD88 dependent induction of IL-12/IFN-gamma synthesis during infection with T. cruzi
Miro et al., J Immunol 2006 : Unraveling the mechanisms underlying this deficiency, we showed that IL-12 signaling in T cells is required to induce expression of costimulatory molecules and secretion of IL-12 by DCs and that IFNGR expression is required on both DCs and CD4+ T cells to induce IL-12 secretion by DCs
He et al., J Immunol 2006 : In peripheral blood monocytes and the THP-1 monocytic cell line, SAA induces the expression of IL-12p40 , a subunit shared by IL-12 and IL-23 ... SAA stimulated expression of IL-12p40 was rapid ( < or = 4 h ), sustainable ( > or = 20 h ), potent ( up to 3380 pg/ml/10 ( 6 ) cells in 24 h ), and insensitive to polymyxin B treatment ... The SAA stimulated IL-12p40 secretion required de novo protein synthesis and was accompanied by activation of the transcription factors NF-kappaB and C/EBP ... Expression of IL-12p40 required activation of the p38 MAPK and PI3K ... Expression of IL-12p40 required activation of the p38 MAPK and PI3K ... Interestingly, the SAA induced IL-12p40 production was accompanied by a sustained expression of IL-23p19, but not IL-12p35, resulting in preferential secretion of IL-23, but not IL-12
Kosaka et al., Cancer Sci 2006 : Moreover, ASGM1 ( + ) CD8 ( + ) T cells, but not ASGM1 ( - ) CD8 ( + ) T cells, produced high levels of interferon (IFN)-gamma in response to IL-12 plus IL-2 ... Moreover, ASGM1 ( + ) CD8 ( + ) T cells, but not ASGM1 ( - ) CD8 ( + ) T cells, produced high levels of interferon (IFN)-gamma in response to IL-12 plus IL-2
Kuranaga et al., J Infect Dis 2006 (Pneumococcal Infections...) : We demonstrated that, although S. pneumoniae challenge induced IL-12 production, it did not induce either interferon (IFN)-gamma or IL-18 production in mice with or without a splenectomy ... We demonstrated that, although S. pneumoniae challenge induced IL-12 production, it did not induce either interferon (IFN)-gamma or IL-18 production in mice with or without a splenectomy
Kurlander et al., Transfusion 2006 : FEP- and PS-cultured DCs are similar in phenotype and in some functional measures, but FEP markedly reduces DC production of IL-12 and IL-10
Suzuki et al., Biomed Pharmacother 2006 : Using enzyme linked immunosorbent assays ( ELISAs ) we showed DHMEQ to inhibit LPS induced secretion of IL-6, IL-12 , interleukin-1beta (IL-1beta), and TNF-alpha
Harada et al., Microbiol Immunol 2006 (Leukopenia) : The levels of interferon-(IFN-)gamma, tumor necrosis factor-(TNF-)alpha, granulocyte-macrophage-colony stimulating factor ( GM-CSF ), interleukin-(IL-) 6 and IL-12p70 were significantly increased by SCG in CY-treated mice ... Neutralizing GM-CSF significantly inhibited the induction of IFN-gamma, TNF-alpha and IL-12p70 by SCG ... Neutralizing GM-CSF significantly inhibited the induction of IFN-gamma, TNF-alpha and IL-12p70 by SCG ... Blocking dectin-1 significantly inhibited the induction of TNF-alpha and IL-12p70 production by SCG ... Blocking dectin-1 significantly inhibited the induction of TNF-alpha and IL-12p70 production by SCG
Lomáková et al., Folia Microbiol (Praha) 2006 : On the other hand, BF had only small effect on IFN-gamma production and no detectable effect on IL-12 production
Melchers et al., Arthritis Rheum 2006 (Arthritis, Rheumatoid...) : Preincubation of these cells with a 16-mer C-type decoy oligonucleotide fully reconstituted the defective IL-10 induced suppression of IL-12 synthesis
Tsujimoto et al., Immunol Lett 2006 : LPS treated DCs could not produce IL-12p70 in response to subsequent both LPS- and CpG DNA-stimulation ... On the other hand, poly ( I:C ) -treated DCs retained to produce IL-12p70 by subsequent CpG DNA-stimulation, while subsequent LPS-stimulation did not induce IL-12p70 production
Berenson et al., J Immunol 2006 : In the present study, we compared the capacity of IL-12 and IFN-alpha to induce Th1 differentiation, STAT4 phosphorylation, and IFN-gamma production in murine T cells ... In the present study, we compared the capacity of IL-12 and IFN-alpha to induce Th1 differentiation, STAT4 phosphorylation, and IFN-gamma production in murine T cells ... In the present study, we compared the capacity of IL-12 and IFN-alpha to induce Th1 differentiation, STAT4 phosphorylation, and IFN-gamma production in murine T cells
Matsuda et al., Int J Oncol 2006 (Disease Models, Animal...) : We found that mAb treatment activates Kupffer cells to produce inflammatory cytokines such as TNF-alpha and IL-12 , and induces the expression of FasL on Kupffer and NKT cells
Longman et al., Blood 2007 : Importantly, STAT1 signaling resulted in significant inhibition of CD40L induced IL-12 production, accounting for the inhibition of CD8+ T-cell activation
Price et al., Immunology 2007 : We hypothesize that the induction of IFN-gamma secretion from WC1 ( + ) gammadelta T cells by IL-12 and IL-18 is likely to be an important element of the innate response to pathogens such as Mycobacterium bovis
Matsubara et al., Am J Respir Cell Mol Biol 2007 (Inflammation...) : These data demonstrate that the combination of IL-2 and IL-18 prevents AHR and airway inflammation, likely through IL-12 mediated induction of IFN-gamma production in NK cells
Jia et al., Am J Physiol Heart Circ Physiol 2007 (Carotid Artery Diseases...) : Here we assessed the expression of IGF-I receptor (IGF-IR) in atherosclerotic plaques and in plaque VSMCs of asymptomatic and symptomatic patients with carotid stenosis and examined the effect of IGF-I, IL-12 , and IFN-gamma on the expression of IGF-IR and IGF binding protein (IGFBP)-3 in plaque VSMCs ... Here we assessed the expression of IGF-I receptor (IGF-IR) in atherosclerotic plaques and in plaque VSMCs of asymptomatic and symptomatic patients with carotid stenosis and examined the effect of IGF-I, IL-12 , and IFN-gamma on the expression of IGF-IR and IGF binding protein (IGFBP)-3 in plaque VSMCs ... IL-12 and IFN-gamma decreased IGF-IR mRNA transcripts and further increased the expression of IGFBP-3 in asymptomatic VSMCs but had no effect in symptomatic VSMCs
Gattoni et al., Clin Ter 2006 (Hepatitis C) : Each of these cell types secretes IFN-gamma only when activated, usually as part of immune response and especially in response to IL-2 and IL-12
Meyers et al., Cytokine 2006 (Lupus Erythematosus, Systemic...) : In summary, pretreatment with either rhIFN-alpha/beta or CpG-A inhibits PBMC secretion of superantigen induced IFN-gamma and IL-17, and CD40L induced IL-12p70 and IL-23 ... Data indicate that CpG-A induced type I IFN inhibit IL-12p70 dependent PBMC IFN-gamma secretion by enhancing IL-10
Martínez del Hoyo et al., J Immunol 2006 : Studies on Fms-like tyrosine kinase 3 ligand bone marrow differentiated B220 ( - ) DCs confirmed that PrP ( C ) expression followed that of MHC class II and costimulatory molecules, and correlated with IL-12 production in response to TLR-9 engagement by CpG
Oumouna et al., J Immunol 2006 (Asthma...) : Although PARP-1 inhibition by gene knockout increased the production of the Th1 cytokines IL-2 and IL-12 , the inhibition by TIQ-A exerted no effect on these two cytokines
Lee et al., J Cell Physiol 2007 (Dermatitis, Contact...) : Silibinin was shown to significantly suppress the expression of CD80, CD86, MHC class I, and MHC class II in the DCs, and was also associated with impairments of LPS induced IL-12 expression in the DCs
Newton et al., Infect Immun 2007 (Disease Progression...) : In the current study, L. pneumophila growth in dendritic cells from BALB/c and A/J mice was examined along with the role of TLR9 in the induction of IL-12 in these cells
Peng et al., J Immunother 2006 : Monocyte derived DC primed with TLR agonists secrete IL-12p70 in a CD40 dependent manner under hyperthermic conditions ... Thus, DC primed with toll-like receptor-agonists respond to hyperthermia with increased IL-12p70 secretion, mediated by heat-shock protein binding and activation of CD40
Brummer et al., Cytokine 2006 (Blastomycosis...) : Methods, results : BALB/c bronchoalveolar and peritoneal macrophages ( PM ) were shown deficient in IL-12 production in response to IFN-gamma+LPS ... Methods, results : BALB/c bronchoalveolar and peritoneal macrophages ( PM ) were shown deficient in IL-12 production in response to IFN-gamma+LPS
Kitawaki et al., Int Immunol 2006 (Dermatitis, Atopic...) : Activated MCs induced maturation of DCs, and potently suppressed IL-12p70 production by the DCs
Kang et al., Leukemia & lymphoma 2006 (Leukemia) : Inhibition of cellular VEGF levels increased interleukin (IL)-12 production and the allostimulatory capacity of leukemic DCs
Murugaiyan et al., J Immunol 2006 (Neoplasms, Experimental) : IL-10 prevents the CD40 induced CTL and TNF-alpha and IL-12 production, Th1 skewing, and tumor regression ... IL-10 prevents the CD40 induced CTL and TNF-alpha and IL-12 production, Th1 skewing, and tumor regression
Kilinc et al., J Immunol 2006 (Adenocarcinoma, Bronchiolo-Alveolar...) : IL-12/GM-CSF treatment promoted a rapid up-regulation of CD43 and CD69 on CD8+ effector/memory T cells, augmented their ability to produce IFN-gamma , and restored granzyme B expression ... Both CD8+ T cell activation and T suppressor cell purge were mediated primarily by IL-12 and required IFN-gamma
Naujokat et al., Immunology 2007 : Inhibition of chymotrypsin-like peptidase activity abrogates macropinocytosis and receptor mediated endocytosis of macromolecular antigens in immature DCs, and inhibits the synthesis of interleukin (IL)-12p70 and IL-12p40 in mature DCs
Yan et al., Life Sci 2007 (Fever) : Blockade of the autocrine action of IL-10 could increase LPS induced TNF-alpha and IL-12 production in DCs
Kawanokuchi et al., Mult Scler 2006 : Here, we show for the first time that murine microglia produce IFN-gamma in response to IL-12 and/or IL-18, using RT-PCR detection of IFN-gamma mRNA and Western blotting and immunohistochemical analysis for cytoplasmic expression of IFN-gamma ... Stimulation of microglia with IL-12 and IL-18 resulted in MHC class II mRNA expression in microglia
Tyler et al., Mol Immunol 2007 : Although IL-12 induced STAT4 activation is a conserved pathway across species, IFN-alpha/beta dependent STAT4 phosphorylation does not occur as efficiently in mice as it does in human T cells
Mitchell et al., Infect Immun 2007 (Helicobacter Infections) : While very little or no IL-12 was produced to H. pylori alone, simultaneous ligation of CD40 on DCs induced IL-12 release
Takemoto et al., J Immunol 2006 (Listeriosis) : Cutting Edge : IL-12 inversely regulates T-bet and eomesodermin expression during pathogen induced CD8+ T cell differentiation
Boonstra et al., J Immunol 2006 : IL-12p70 expression in response to CpG was suppressed by endogenous IL-10 in macrophages, in myeloid DC, and to an even greater extent in splenic CD8alpha ( - ) and CD8alpha ( + ) DC
Owaki et al., J Immunol 2006 : Finally, IL-12Rbeta2 expression was shown to be up-regulated by IL-27 in both T-bet- and ICAM-1/LFA-1 dependent mechanisms
Li et al., J Immunol 2006 : Moreover, the IL-12 induced STAT4 engenders greater clonal expansion of the Ag-activated CD8 cells by regulating the expression of the transcriptional factor Bcl3- and Bcl2 related genes that promote survival of Ag-activated CD8 cells
Dawson et al., BMC immunology 2006 : Stimulation of human PBMCs and purified T cells with ATRA and 9-cis-RA increased mRNA and protein levels of IL-4, IL-5, and IL-13 and decreased levels of IFN-gamma, IL-2, IL-12p70 and TNF-alpha upon activation with anti-CD3 and/or anti-CD28 mAbs
Koch et al., Respir Med 2007 (Asthma) : IL-12 induced T-bet expression and IFNgamma release in lymphocytes from asthmatics -- role of MAPkinases ERK-1/-2, p38 ( MAPK ) and effect of dexamethasone ... The transcription factor T-box-expressed-in-T-cells ( T-bet ) is required for T ( H ) 1 lymphocyte differentiation, regulates the IL-12 induced expression of the T ( H ) 1-specific cytokine IFNgamma and may be dysregulated in asthmatics ... The modulatory role of extracellular signal regulated kinase ( ERK ) -1/-2, p38mitogen activated protein kinase ( MAPK ) and dexamethasone on IL-12 induced T-bet and IFNgamma expression was assessed in peripheral blood lymphocytes of 10 atopic asthmatics and 10 nonatopic normals ... The modulatory role of extracellular signal regulated kinase ( ERK ) -1/-2, p38mitogen activated protein kinase ( MAPK ) and dexamethasone on IL-12 induced T-bet and IFNgamma expression was assessed in peripheral blood lymphocytes of 10 atopic asthmatics and 10 nonatopic normals ... The modulatory role of extracellular signal regulated kinase ( ERK ) -1/-2, p38mitogen activated protein kinase ( MAPK ) and dexamethasone on IL-12 induced T-bet and IFNgamma expression was assessed in peripheral blood lymphocytes of 10 atopic asthmatics and 10 nonatopic normals ... Dexamethasone blocked the IL-12 induced T-bet expression in asthmatic T-cells completely and decreased IL-12 induced IFNgamma release by approximately 50 %, which occurred to the same extent in asthmatic and normal T-cells
Castanheira et al., Frontiers in bioscience : a journal and virtual library 2007 : IFN-gamma production was IL-12 dependent with NK cells as the main source
Verma et al., Transplantation 2006 : The mechanism by which IL-12p70 induced delayed rejection was examined by reverse transcription polymerase chain reaction of cytokine mRNA and studying the role of interferon (IFN)-gamma and inducible nitric oxide synthase (iNOS) that were induced by IL-12 ... The mechanism by which IL-12p70 induced delayed rejection was examined by reverse transcription polymerase chain reaction of cytokine mRNA and studying the role of interferon (IFN)-gamma and inducible nitric oxide synthase (iNOS) that were induced by IL-12
Aksoy et al., Blood 2007 : However, addition of exogenous IFNbeta failed to restore LPS induced IL-12p70 synthesis which was previously shown to be defective in neonatal moDCs ... Because IRF-3 activity is also required for IL-12p70 synthesis, our findings provide a molecular basis for the decreased ability of LPS stimulated neonatal moDCs to elicit Th1-type responses
Sahiratmadja et al., Infect Immun 2007 (Tuberculosis, Pulmonary) : Depressed IFN-gamma production was not due to decreased IL-12/IL-23 production
Osterloh et al., J Biol Chem 2007 : Hsp60 specifically binds bacterial LPS and both molecules synergistically enhanced IL-12p40 production in APC and IFNgamma release in antigen dependent T cell activation
Endt et al., Eur J Immunol 2007 : IL-2 stimulation leads to an up-regulation of SAP expression, which can be enhanced by IL-12 , the stimulation of TLR3 by polyinosinic-polycytidylic acid ( poly ( I:C ) ) and to a lesser extent by IFN-alpha
Penna et al., J Immunol 2007 : 1,25 ( OH ) ( 2 ) D ( 3 ) also decreased IL-12p75 production by M-DCs, as expected, and inhibited CCR7 expression
Flores et al., J Immunol 2007 : Our analysis revealed that both LPS and CpG induced IL-10 and IL-12 production but that the presence of IFN-gamma , in a dose dependent manner, suppressed the production of IL-10 while enhancing that of IL-12 ... Our analysis revealed that both LPS and CpG induced IL-10 and IL-12 production but that the presence of IFN-gamma, in a dose dependent manner, suppressed the production of IL-10 while enhancing that of IL-12 ... Experiments performed with STAT-1 knockout mice demonstrated that the primary production of IL-12 induced by CpG was STAT-1 dependent , whereas the production of IL-10 was not
Ranjbaran et al., J Immunol 2007 (Arteritis...) : T cells were the principal source of IFN-gamma in response to IL-12/IL-18 within the arterial wall ... In contrast to cognate stimuli, IL-12/IL-18 dependent IFN-gamma secretion was prevented by a p38 MAPK inhibitor and not by cyclosporine
Zipris et al., J Immunol 2007 (Diabetes Mellitus, Type 1...) : KRV infection induces high levels of IL-12p40 by splenic B cells and Flt-3-ligand induced bone marrow derived dendritic cells (DCs) but only low levels of IL-12p40 production by thioglycolate elicited peritoneal macrophages or GM-CSF plus IL-4 induced bone marrow derived DCs ... Genomic KRV DNA also induces BBDR splenocytes and Flt-3L induced DCs from wild-type but not TLR9-deficient mice to produce IL-12p40 ; KRV induced up-regulation of B lymphocytes can be blocked by TLR9 antagonists including inhibitory CpG and chloroquine
Lasarte et al., J Immunol 2007 (Neoplasms) : EDA also stimulated the production by DC of proinflammatory cytokines such as IL-12 or TNF-alpha and induced their maturation in vitro and in vivo
Bsibsi et al., Glia 2007 : Moreover, the CD14 triggered TLR2 mediated response in astrocytes lead to the production of CXCL8, IL-6, and IL12p40 , whereas typical TLR induced pro-inflammatory cytokines, like TNF-alpha and IL-1beta, were not produced at detectable levels
Wells et al., J Allergy Clin Immunol 2007 (Inflammation...) : Adoptive transfer of transgenic antigen-specific CD8, but not CD4 , cells resulted in increased IL-12 levels and reduced IL-13 and IL-5 levels in bronchoalveolar lavage fluid, coupled with substantially reduced airway eosinophilia after repeated allergen inhalation, a process mimicked by intranasal administration of IL-12 and inhibited by anti-IL-12 antibody ... Adoptive transfer of transgenic antigen-specific CD8 , but not CD4, cells resulted in increased IL-12 levels and reduced IL-13 and IL-5 levels in bronchoalveolar lavage fluid, coupled with substantially reduced airway eosinophilia after repeated allergen inhalation, a process mimicked by intranasal administration of IL-12 and inhibited by anti-IL-12 antibody
Riganò et al., Infect Immun 2007 : SHF and particularly AgB reduced the production of interleukin-12p70 (IL-12p70) and tumor necrosis factor alpha in lipopolysaccharide stimulated iDCs
Ivory et al., Eur J Immunol 2007 (Entamoebiasis) : The Gal-lectin also induced DC production of IL-12 , indicating a Th1 response
Lehmann et al., J Invest Dermatol 2007 : Moreover, inhibition of HO-1 activity restored the diminished IL-12 and IFNgamma production after FAE treatment
Watkins et al., J Immunol 2007 (Carcinoma, Lewis Lung...) : Analysis of tumor infiltrating macrophages and distal TAMs revealed that IL-12 , both in vivo and in vitro, induced a rapid ( < 90 min ) reduction of tumor supportive macrophage activities ( IL-10, MCP-1, migration inhibitory factor, and TGFbeta production ) and a concomitant increase in proinflammatory and proimmunogenic activities ( TNF-alpha , IL-15, and IL-18 production ) ... Analysis of tumor infiltrating macrophages and distal TAMs revealed that IL-12 , both in vivo and in vitro, induced a rapid ( < 90 min ) reduction of tumor supportive macrophage activities ( IL-10, MCP-1, migration inhibitory factor, and TGFbeta production ) and a concomitant increase in proinflammatory and proimmunogenic activities ( TNF-alpha, IL-15 , and IL-18 production ) ... Analysis of tumor infiltrating macrophages and distal TAMs revealed that IL-12 , both in vivo and in vitro, induced a rapid ( < 90 min ) reduction of tumor supportive macrophage activities ( IL-10, MCP-1, migration inhibitory factor, and TGFbeta production ) and a concomitant increase in proinflammatory and proimmunogenic activities ( TNF-alpha, IL-15, and IL-18 production )
Xu et al., J Immunol 2007 (Encephalomyelitis, Autoimmune, Experimental) : In primary astrocytes, LPS induced the production of IL-12p40 , IL-23, and IL-27p28 proteins
Wang et al., J Clin Immunol 2007 : Retinoic acid enhances the production of IL-10 while reducing the synthesis of IL-12 and TNF-alpha from LPS stimulated monocytes/macrophages ... The effects of atRA on LPS induced mRNA expression of IL-10, IL-12p40 , TNF-alpha, IL-18, and TGF-beta in the THP-1 monocyte/macrophage cell line and in cord blood mononuclear cells were measured by competitive RT-PCR ... In contrast, the addition of atRA inhibited the LPS induced TNF-alpha and IL-12p40 mRNA expression, and the number of ELISPOT positive cells for TNF-alpha
Flaminio et al., Journal of immune based therapies and vaccines 2007 : Nevertheless, adult horse DCs , but not macrophages, increased their expression of IL-12 and IFNalpha cytokines upon CpG-ODN stimulation
Akilov et al., J Leukoc Biol 2007 (Disease Models, Animal...) : Inhibition of MP responses is a hallmark of Leishmania infection ; our data demonstrate further that host MR is not essential for blocking IFN-gamma/LPS induced IL-12 production and MAPK activation by Leishmania ... Inhibition of MP responses is a hallmark of Leishmania infection ; our data demonstrate further that host MR is not essential for blocking IFN-gamma/LPS induced IL-12 production and MAPK activation by Leishmania
Goodridge et al., Parasite Immunol 2007 : PC-Ova mimicked the modulation of interleukin (IL)-12 production by ES-62 in a TLR4- and MyD88 dependent manner and as with native ES-62, PC-Ova effects were not dependent on Pro712
Klotz et al., J Immunol 2007 : Sustained PPARgamma activation in murine DCs reduced maturation induced expression of costimulatory molecules and IL-12 , and profoundly inhibited their capacity to prime naive CD4 ( + ) T cells in vitro
Sawaki et al., Int Immunol 2007 : Hepatic CD3 ( - ) DX5 ( + ) NK cells produced IFN-gamma in response to TLR2 or TLR7 agonists only when co-stimulated with IL-12, indicating that TLR agonists synergize with IL-12 for IFN-gamma ... The tlr2 ( -/- ) or tlr7 ( -/- ) NK cells could not produce IFN-gamma in response to IL-12 plus TLR2 or TLR7 ligands, respectively, indicating requirement of the corresponding TLRs
Nakano et al., Blood 2007 (Bacterial Infections...) : IL-12 was secreted from BMMCs in response to LPS but not by FcepsilonRI cross linking
Boddupalli et al., Free Radic Biol Med 2007 : In activated macrophages, the rel/NF-kappaB transcription factors are known to play important roles in interleukin-12 (IL-12) p40 regulation by nitric oxide ( NO ) ... Here, we describe a dominant role for c-rel involving p38 mitogen activated protein kinase ( p38 MAPK ) and calmodulin (CaM) protein in NO-mediated IL-12 p40 inhibition in activated macrophages
Letimier et al., EMBO J 2007 : However, high-level IL-12Rbeta2 expression required TCR triggering in the presence of IL-12
Chang et al., Eur J Immunol 2007 : In Th1 cells, IL-10 expression is induced by IL-12 , but the IL-10 gene lacks detectable histone acetylation ... While in primary Th1 responses IL-12 is required to induce expression of the pro-inflammatory cytokine IFN-gamma , in secondary Th1 responses IFN-gamma re-expression is independent of IL-12, which still is able to induce expression of the anti-inflammatory cytokine IL-10
Jung et al., Int Immunol 2007 : SKI suppressed IL-12 production by DCs and IFN-gamma production by T cells
Nakae et al., J Leukoc Biol 2007 : We found that IL-23 or IL-17 can suppress Th1 cell differentiation in the presence of exogenous IL-12 in vitro ... We found that IL-23 or IL-17 can suppress Th1 cell differentiation in the presence of exogenous IL-12 in vitro
Takahashi et al., Br J Pharmacol 2007 : In the present study, we examined the effect of adenosine on the IL-18 induced up-regulation of ICAM-1 on human monocytes and production of IL-12 , IFN-gamma and TNF-alpha by PBMC
Nagarajan et al., J Immunol 2007 : IFN-gamma production by NKT cells is dependent on LPS induced IL-12 and IL-18 from APC ... IFN-gamma production by NKT cells is dependent on LPS induced IL-12 and IL-18 from APC
Uto et al., J Immunol 2007 (Listeriosis) : In vivo, gamma-PGA NPs were preferentially internalized by APCs ( DCs and macrophages ) and induced the production of IL-12p40 and IL-6
Cheng et al., J Neuroimmunol 2007 (Encephalomyelitis, Autoimmune, Experimental) : Similarly, IL-12 induces IFN-gamma and PD-L1 expression in cultured MOG-specific T cells from wild-type mice but not from IFN-gamma-deficient mice ... Similarly, IL-12 induces IFN-gamma and PD-L1 expression in cultured MOG-specific T cells from wild-type mice but not from IFN-gamma-deficient mice
Ashenager et al., J Investig Allergol Clin Immunol 2007 : RT-PCR revealed that IL-12 induced expression of IFN-gamma was partially suppressed by loratadine and fexofenadine and that all 4 agents tested inhibited IL-4 induced expression of IL-5 ... ELISA demonstrated that IL-12 induced IFN-gamma production was significantly suppressed by cetirizine and fexofenadine and IL-4 induced IL-5 production was downregulated by three agents with the exception of cetirizine
Lu et al., Immunol Res 2006 (Bacterial Infections...) : We and others have shown that, in TH1 cells, Gadd45b and Gadd45g are induced by TCR signaling or IL-12 and IL-18 ... We and others have shown that, in TH1 cells, Gadd45b and Gadd45g are induced by TCR signaling or IL-12 and IL-18
Wolf et al., J Neuroimmunol 2007 (Encephalomyelitis, Autoimmune, Experimental) : Spleen cells proliferated and secreted IFN-gamma in response to MBP ( 68-86 ), and secreted IL-6 and IL-12p40 in response to CpG-ODN and LPS
Yuminamochi et al., Immunology 2007 : These results clearly demonstrated that A. blazei and ABPC augmented NK cell activation through IL-12 mediated IFN-gamma production
Ma et al., J Biol Chem 2007 (Calcium Signaling) : Cyclosporin A and FK506 inhibit IL-12p40 production through the calmodulin/calmodulin dependent protein kinase activated phosphoinositide 3-kinase in lipopolysaccharide stimulated human monocytic cells
Mahanonda et al., Asian Pac J Allergy Immunol 2006 : LPS from all three bacteria induced only negligible IL-12 production
Fahey et al., J Leukoc Biol 2007 : Reciprocal effects of IFN-beta and IL-12 on STAT4 activation and cytokine induction in T cells ... We report that IFN-beta increases IL-12 induced STAT4 phosphorylation and up-regulates IL-12 receptor beta1 and beta2 expression ... However, despite this up-regulation, IFN-beta suppressed IL-12 induced IFN-gamma expression
Hsu et al., J Allergy Clin Immunol 2007 : Le ( x ) and an isomer, Le ( y ), but not other isomers, inhibited the production of LPS induced IL-12p70 , associated with a significant reduction of nuclear NF-kappaB, in bone marrow derived dendritic cells from BALB/c mice, suggesting that Le ( x ) -induced suppression of IL-12p70 results in an enhanced T ( H ) 2 response
Kariya et al., Am J Otolaryngol 2007 (Mucocele...) : IL-12 was detected in all samples, whereas IL-10 was detected in only one case
Lee et al., J Leukoc Biol 2007 : Incubation of p110alpha-deficient THP-1 cells with LPS showed that p110alpha was required for IL-12p40 and IL-6 production, whereas it negatively regulated the production of TNF-alpha and IL-10
Ceballos et al., Immunology 2007 : Consistent with these results, we also observed that SPC stimulated the production of interleukin (IL)-12 and IL-18 by DCs
Liu et al., J Exp Med 2007 (Toxoplasmosis) : DGKzeta deficiency results in impaired interleukin (IL) 12 and tumor necrosis factor alpha production following TLR stimulation in vitro and in vivo, increased resistance to endotoxin shock, and enhanced susceptibility to Toxoplasma gondii infection ... We further show that DGKzeta negatively controls the phosphatidylinositol 3-kinase (PI3K)-Akt pathway and that inhibition of PI3K activity or treatment with PA can restore lipopolysaccharide induced IL-12 production by DGKzeta-deficient Mphi ... We further show that DGKzeta negatively controls the phosphatidylinositol 3-kinase (PI3K)-Akt pathway and that inhibition of PI3K activity or treatment with PA can restore lipopolysaccharide induced IL-12 production by DGKzeta-deficient Mphi
Way et al., J Immunol 2007 (Listeriosis) : In this study, we examine the requirements for IL-12 and IFN-I, either individually or in combination, for priming Ag-specific T cell IFN-gamma production after Listeria monocytogenes ( Lm ) infection ... The additive effects of IL-12 and IFN-I signaling on IFN-gamma production by CD4 T cells were further demonstrated by adoptive transfer of transgenic IFN-IR ( +/+ ) and IFN-IR ( -/- ) CD4 T cells into normal and IL-12-deficient mice, and infection with rLm
Messmer et al., Mol Med 2006 (Opioid-Related Disorders) : The change in surface phenotype was paralleled by a p38 MAPK dependent decrease in IL-10 and increase in IL-12 secretion
Csomor et al., Mol Immunol 2007 : Immobilized C1q induced maturation of MDCs and enhanced secretion of IL-12 and TNF-alpha, moreover, elevated their T-cell stimulating capacity
Nishinakamura et al., Int Immunol 2007 : Activated STAT3 suppresses LPS induced IL-6, tumor necrosis factor-alpha and IL-12 gene expression in macrophages and dendritic cells
Schleicher et al., J Exp Med 2007 (Leishmaniasis, Visceral) : In vitro, pDCs did not endocytose Leishmania promastigotes but nevertheless released interferon (IFN)-alpha/beta and interleukin (IL)-12 in a TLR9 dependent manner
Kesherwani et al., Cytokine 2007 : Quantitative role of p42/44 and p38 in the production and regulation of cytokines TNF-alpha, IL-1beta and IL-12 by murine peritoneal macrophages in vitro by concanavalin A ... Quantitative role of p42/44 and p38 in the production and regulation of cytokines TNF-alpha, IL-1beta and IL-12 by murine peritoneal macrophages in vitro by concanavalin A ... Quantitative role of p42/44 and p38 in the production and regulation of cytokines TNF-alpha, IL-1beta and IL-12 by murine peritoneal macrophages in vitro by concanavalin A ... In the present study the quantitative role of p42/44 and p38 in the production of TNF-alpha, IL-1beta and IL-12 by murine peritoneal macrophages, in vitro, on treatment with Concanavalin A ( ConA ) has been investigated ... In the present study the quantitative role of p42/44 and p38 in the production of TNF-alpha, IL-1beta and IL-12 by murine peritoneal macrophages, in vitro, on treatment with Concanavalin A ( ConA ) has been investigated ... In the present study the quantitative role of p42/44 and p38 in the production of TNF-alpha, IL-1beta and IL-12 by murine peritoneal macrophages, in vitro, on treatment with Concanavalin A ( ConA ) has been investigated ... The expression of TNF-alpha, IL-1beta and IL-12 was down regulated in the presence of PD98059 and SB202190 in a dose dependent manner, suggesting the involvement of p42/44 and p38 in ConA induced production of TNF-alpha, IL-1beta and IL-12 by macrophages ... The expression of TNF-alpha, IL-1beta and IL-12 was down regulated in the presence of PD98059 and SB202190 in a dose dependent manner, suggesting the involvement of p42/44 and p38 in ConA induced production of TNF-alpha, IL-1beta and IL-12 by macrophages ... The expression of TNF-alpha, IL-1beta and IL-12 was down regulated in the presence of PD98059 and SB202190 in a dose dependent manner, suggesting the involvement of p42/44 and p38 in ConA induced production of TNF-alpha, IL-1beta and IL-12 by macrophages
Tokumasa et al., Blood 2007 : Expression of Tyk2 in dendritic cells is required for IL-12 , IL-23, and IFN-gamma production and the induction of Th1 cell differentiation ... While the differentiation and maturation of DCs was normal in Tyk2-deficient ( Tyk2 ( -/- ) ) mice, IL-12 induced Stat4 phosphorylation was diminished in Tyk2 ( -/- ) DCs ... IL-12 induced IFN-gamma production was also significantly diminished in Tyk2 ( -/- ) DCs to levels similar to those in Stat4 ( -/- ) DCs
Yeh et al., Life Sci 2007 (Edema...) : Hesperidin downregulate the LPS induced expression of TNF-alpha, IL-1 beta, IL-6, KC, MIP-2, MCP-1, and IL-12
Moeinpour et al., Mol Immunol 2007 (Hemorrhage...) : Trauma-hemorrhage increased the production of IL-6, IL-10, IL-12 and TNF-alpha enhanced the expression of TLR4 , MyD88 as well as the activation of MAPK proteins ( p38, ERK and JNK ) in epidermal keratinocytes
de Arquer et al., J Leukoc Biol 2007 (HIV Infections) : IL-12 and IL-18 synergistically induce the production of IFN-gamma by resting and activated T cells ... Higher IL-12Rbeta1 and IL-18Ralpha expression correlated ( r=0.87 ; P < 0.007 ) to increased production of IFN-gamma by isolated CD4 T cells in the presence of IL-12 and IL-18 ... Moreover, exogenous IL-12 and IL-18 induced the up-regulation of IL-12Rbeta2 to twice higher in CD4 T cells from HIV-1 positive individuals compared with controls
Smeltz et al., J Immunol 2007 : Comparison of IL-15 and IL-7 demonstrated that IL-15 was superior in its ability to enhance IL-12-IL-18 induced IFN-gamma , without evidence of a synergistic effect between IL-15 and IL-7 ... We also observed that IL-15- and IL-7 mediated enhancement of IL-12-IL-18 induced IFN-gamma production was a functional property of effector memory CD8 ( + ) T cells ... Despite a lack of association between cell division and acquisition of IL-12-IL-18 induced IFN-gamma , down-regulation of CD62L expression correlated well with increased IL-12-IL-18 induced IFN-gamma ... Purified central memory T cells stimulated with IL-15 and IL-7 down-regulated CD62L and acquired potent IL-12-IL-18 induced IFN-gamma similar to effector memory T cells
Numasaki et al., J Immunol 2007 (Fibrosarcoma...) : IL-28 increased the total number of splenic NK cells in SCID mice and enhanced IL-12 induced IFN-gamma production in vivo and expanded spleen cells in C57BL/6 mice
Pompei et al., J Immunol 2007 (Tuberculosis) : Removal of IL-10 or addition of IFNgamma enhances macrophage IL-12 release to Mtb, but without affecting the kinetics of remodeling at the macrophage p40 promoter ... Our results also imply that TLR2 signaling is not a good inducer of IL-12 , supporting the increasingly strong paradigm that TLR2 favors Th2 responses
Kim et al., J Immunol 2007 (Asthma...) : During the allergen sensitization period, TNF-alpha expression was found to be enhanced by both low- and high-dose LPS, whereas IL-12 expression was only enhanced by high-dose LPS
Stefanova et al., J Exp Ther Oncol 2007 (Sarcoma 180) : In vitro, coumarins induced IL-12 in murine macrophages and additively increased the LPS induced IL-12 release
Ishida-Fujii et al., Biosci Biotechnol Biochem 2007 (Escherichia coli Infections) : It also enhanced LPS stimulated IL-12 and TNF-alpha production, but not IL-6 production
Bhol et al., Dig Dis Sci 2007 (Colitis, Ulcerative...) : NPI 32101 significantly suppressed the expression of matrix metalloproteinase (MMP)-9, tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, and IL-12 , whereas sulfasalazine suppressed MMP-9, IL-1beta, and TNF-alpha, but not IL-12, compared to placebo
Soares et al., J Exp Med 2007 : Another major DC subset, targeted with 33D1 anti-DCIR2 antibody, also induced IFN-gamma in vivo but required IL-12 , not CD70
Zhu et al., J Immunol 2007 (Disease Models, Animal...) : The Pro-Th1 cytokine IL-12 enhances IL-4 production by invariant NKT cells : relevance for T cell mediated hepatitis ... We demonstrate that IL-12 interacts directly with iNKT cells, contributes to their recruitment to the liver, and enhances their IL-4 production, which is essential for disease onset
Fichtner-Feigl et al., J Immunol 2007 (Colitis...) : In vitro stimulation studies suggest that this IL-13 production is dependent on IL-23 and IL-25, but not on IL-12p70
Charmoy et al., J Leukoc Biol 2007 (Disease Susceptibility) : Thus, following infection with L. major, in C57BL/6 mice, PMN could constitute one of the earliest sources of IL-12, and in BALB/c mice, secretion of IL-12p40 could contribute to impaired, early IL-12 signaling
Gutermuth et al., J Allergy Clin Immunol 2007 : In vitro Bet-APEs and E ( 1 ) -phytoprostanes dose-dependently inhibited LPS induced IL-12p70 of DCs
Yin et al., J Toxicol Environ Health 2007 (Listeriosis) : DEP or eDEP concentration-dependently suppressed L. monocytogenes induced secretion of tumor necrosis factor-alpha, interleukin (IL)-1beta, and IL-12 by AM and of IL-2 and interferon-gamma by lymphocytes obtained from L. monocytogenes infected rats, but augmented the AM secretion of IL-10
Pawar et al., J Am Soc Nephrol 2007 (Lupus Nephritis...) : Only IRS 661 but not IRS 954 significantly reduced serum levels of IL-12p40 , anti-dsDNA IgG ( 2a ), IgG ( 2b ), and anti-Smith IgG
Vuckovic et al., Clin Immunol 2007 (Celiac Disease...) : Blood DC in T1D patients were not distinguished from those of controls by the levels of HLA-DR, CD40 and CD86 expression or the percentage of DC expressing cytokines, IL-12 , IL-10, IL-6 and TNF-alpha, in responses to poly I:C
Kim et al., Immunopharmacol Immunotoxicol 2007 : The acetone fraction prepared from bamboo, Phyllostachys nigra var. henonis, potently inhibited the Lipo polysaccharide (LPS) induced IL-12 production from RAW264.7 monocytic cell-line in a dose dependent manner
Ripoll et al., J Immunol 2007 : Gpnmb overexpression in RAW264.7 cells caused a 2-fold reduction in the production of the cytokines IL-6 and IL-12p40 and the inflammatory mediator NO in response to LPS ... Gpnmb overexpression in RAW264.7 cells caused a 2-fold reduction in the production of the cytokines IL-6 and IL-12p40 and the inflammatory mediator NO in response to LPS
Stewart et al., Eur J Immunol 2007 : `` NK-like gammadelta T cells '' are CD127+, have a memory activated phenotype, express multiple NK cell receptors and readily produce interferon-gamma in response to IL-12/IL-18 stimulation
Koike et al., Am J Respir Cell Mol Biol 2007 (Asthma...) : In vitro, gamma-GCE pretreatment of human monocytic THP-1 cells elevated the GSH/GSSG ratio and enhanced IL-12 ( p70 ) production induced by LPS
Hiraki et al., Am J Surg 2007 (Digestive System Neoplasms...) : IL-2- and IL-12 induced IFN-gamma production by PBMCs was suppressed significantly in both the OP ( POD5 ) and SP groups compared with that in healthy controls
Gao et al., Toxicol Appl Pharmacol 2007 : After LPS stimulation, IL-6, IL-10, IL-12 ( p70 ), and TNF-alpha levels significantly increased with both Pb-DCs and DCs, but Pb-DCs produced significantly less cytokines than did DCs, except for IL-10, which further supports Pb-DC preferential skewing toward type-2 immunity
Imanishi et al., J Immunol 2007 : In mouse Th1 cells the stimulation by TLR2 but not by other TLRs directly induced IFN-gamma production, cell proliferation, and cell survival without TCR stimulation, and these effects were greatly enhanced by IL-2 or IL-12 through the enhanced activation of MAPKs
Pedra et al., FEMS Immunol Med Microbiol 2007 (Anaplasmosis...) : The lack of IL-12/23p40 reduced IFN-gamma production in both CD4 ( + ) and CD8 ( + ) T cells although the effect was more pronounced in CD4 ( + ) T cells
Bastos et al., J Interferon Cytokine Res 2007 : Besides the established role of interleukin-12 (IL-12) and IL-18 on interferon-gamma (IFN-gamma) production by natural killer ( NK ), T, and B cells, the effects of these cytokines on macrophages are largely unknown ... Here, we investigated the role of IL-12/IL-18 on nitric oxide ( NO ) and tumor necrosis factor-alpha (TNF-alpha) production by CD11b ( + ) adherent peritoneal cells, focusing on the involvement of endogenously produced IFN-gamma
Ivanov et al., Blood 2007 : CpG-ODN stimulates macrophages and dendritic cells to secrete HMGB1 ; in turn, extracellular HMGB1 accelerates the delivery of CpG-ODNs to its receptor, leading to a TLR9 dependent augmentation of IL-6, IL-12 , and TNFalpha secretion ... Loss of HMGB1 leads to a defect in the IL-6, IL-12 , TNFalpha, and iNOS response to CpG-ODN
Casey et al., J Immunol 2007 : Furthermore, IL-21 fails to induce IFN-gamma or IL-4 production and can partially block IL-12 induction of IFN-gamma production ... Furthermore, IL-21 fails to induce IFN-gamma or IL-4 production and can partially block IL-12 induction of IFN-gamma production
Sheibanie et al., J Immunol 2007 (Colitis...) : We assessed the effects of PGE ( 2 ) on IL-12, IL-27, and IL-23 and found that PGE ( 2 ) promotes IL-23, inhibits IL-12 and IL-27 expression and release from stimulated DCs, and subsequently induces IL-17 production in activated T cells
Kulaber et al., Rheumatol Int 2007 (Behcet Syndrome...) : Finally, IPP stimulated higher IL-12 secretions from PBMC in BD patients ( P = 0.035 ) and in patients with ( P = 0.02 ) or without ( P = 0.017 ) uveitis or arthritis ( P = 0.04 ), under colchicum treatment ( P = 0.01 ) or not receiving any immunosuppressive treatment ( P = 0.007 ) compared to HC
Ahmed et al., J Vet Med Sci 2007 : Leptin treatment augmented the monocyte/macrophages mRNA expression of only TNF-alpha and IL-12p40 to comparable levels of cells treated with lipopolysaccharide (LPS)
Tsuboi et al., Clin Immunol 2007 (Shwartzman Phenomenon) : The fact that Gal-9 does not suppress LPS induced TNF-alpha, IFN-gamma and IL-12 production in neutropenic mice, and that it does not protect those mice from the Shwartzman reaction, confirms the involvement of PMN in regulation ... The fact that Gal-9 does not suppress LPS induced TNF-alpha, IFN-gamma and IL-12 production in neutropenic mice, and that it does not protect those mice from the Shwartzman reaction, confirms the involvement of PMN in regulation
Eksioglu et al., Exp Hematol 2007 (Inflammation) : We investigated the hypothesis that granulocyte-macrophage colony stimulating factor ( GM-CSF ) induces IL-12 production and polarizes T lymphocytes toward a proinflammatory response
Ghosh et al., Int Immunopharmacol 2007 : TLR4-TLR7/8 combinations synergistically up-regulated IFN-gamma and IL-12 , enhanced IFN-alpha and also moderately induced TNF-alpha
Yang et al., Blood 2007 : Together, we provide a novel mechanism for T-bet regulation, and suggest that IL-12/Stat4/T-bet play an important role in CD8 effector responses
Usluoglu et al., Eur J Immunol 2007 : IL-12 , the critical factor for the generation of the Th1 type immune response, is produced by dendritic cells ( DC ) upon stimulation with LPS ... Different signal pathways mediate LPS induced expression of IL-12 and involve PI3K, MAPK and the transcription factor NF-kappaB ... Different signal pathways mediate LPS induced expression of IL-12 and involve PI3K, MAPK and the transcription factor NF-kappaB ... Different signal pathways mediate LPS induced expression of IL-12 and involve PI3K, MAPK and the transcription factor NF-kappaB ... Different signal pathways mediate LPS induced expression of IL-12 and involve PI3K , MAPK and the transcription factor NF-kappaB
Kong et al., J Immunol 2007 : Ectopic expression of Ro52 enhanced IL-12p40 expression in IFN-gamma/TLR stimulated macrophages in an IRF-8 dependent manner
Polumuri et al., J Immunol 2007 : Role of phosphatidylinositol-3 kinase in transcriptional regulation of TLR induced IL-12 and IL-10 by Fc gamma receptor ligation in murine macrophages ... Because PI3K deficiency has been associated with increased IL-12, we hypothesized that PI3K was central to the anti-inflammatory effect of FcgammaR ligation on TLR induced IL-12 ... This increase was blocked by PI3K inhibitors, wortmannin or LY294002, as was the effect of FcgammaR ligation on TLR induced IL-12 and IL-10 ... Thus, FcgammaR ligation modulates LPS induced IL-12 via multiple PI3K-sensitive pathways that affect production, accumulation, and binding of key DNA binding proteins required for IL-12 induction
Aldinucci et al., J Immunol 2007 : In the same cultures, PARP-1 inhibitors also reduced production of IL-12 and IL-10 ... In the same cultures, PARP-1 inhibitors also reduced production of IL-12 and IL-10
Orozco et al., J Dent Res 2007 (Gingivitis...) : In the presence of IL-12 , IL-18 induces Th1 responses while, in the absence of IL-12, it promotes Th2 responses
Fan et al., J Immunother 2007 (Melanoma, Experimental...) : We demonstrate that TGFbeta-resistant T cells are capable of sustaining IL-12 induced mitogenesis and interferon-gamma secretion in a TGFbeta-rich milieu
Foster et al., J Immunother 2007 (Cytomegalovirus Infections) : Activated T-APC readily reactivated CD8 pp65 memory T cells from healthy CMV seropositive donors ; however, the activation of MAGE-3 and MART-1-specific CD8 T cells required both IL-7 and IL-12 , which could be provided either exogenously or by genetic modification of the T-APC
Zhang et al., Immunol Cell Biol 2007 : It was observed that IL-12 induced release of IL-4, but reduced trypsin- and tryptase stimulated IL-4 secretion from P815 cells ... PD98059, U0126 and LY294002 not only abolished IL-12 induced IL-4 release but also inhibited IL-12 induced phosphorylation of extracellular signal regulated kinase and Akt
Temblay et al., J Allergy Clin Immunol 2007 (Food Hypersensitivity) : Peyer patch-DCs but not splenic DCs from sensitized C3H/HeJ but not Balb/c mice produced less IL-4 dependent IL-12p70 ... In vivo data confirmed this was restricted to the gut immune system, and it was not linked to reduced expression of IL-4 receptor or the lack of functional Toll-like receptor 4 ; instead, IL-4 failed to inhibit IL-10 production by PP-DCs, a pathway critically involved in IL-4 dependent production of IL-12p70
Setiawan et al., Infect Immun 2007 (Strongylida Infections) : IL-10 blockade in vitro with anti-IL-10 receptor (IL-10R) monoclonal antibody restored LPMC IFN-gamma and IL-12 p40 secretion
Yin et al., Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2007 : [ Effect of TLR ligand ( R-848 ) and IL-12 on the production of IFN-gamma by human NK cell subsets ] ... To investigate the effect of the TLR ligand ( R-848 ) and IL-12 on the production of IFN-gamma by human NK cell subsets
Stober et al., Infect Immun 2007 : DCs from mutant and congenic wild-type mice upregulate interleukin-12 (IL-12) and IL-10 mRNA in response to lipopolysaccharide (LPS) stimulation, but the ratio of IL-10 to IL-12 is higher in unstimulated DCs and DCs stimulated for 15 h with LPS from mutant mice than from wild-type mice
Olleros et al., Immunology 2007 (Granuloma...) : Interleukin (IL)-12p40 , a subunit of IL-12p70 and IL-23, has previously been shown to inhibit IL-12p70 activity and interferon-gamma (IFN-gamma) production ... Interleukin (IL)-12p40 , a subunit of IL-12p70 and IL-23, has previously been shown to inhibit IL-12p70 activity and interferon-gamma (IFN-gamma) production ... Interleukin (IL)-12p40 , a subunit of IL-12p70 and IL-23, has previously been shown to inhibit IL-12p70 activity and interferon-gamma (IFN-gamma) production
Fan et al., Clinical and vaccine immunology : CVI 2007 (HIV Infections...) : Blocking of IL-10 with an anti-IL-10 monoclonal antibody in the CD40L stimulated DC cultures from HCV infected persons increased the level of IL-12 p70 production
Zhu et al., Clin Cancer Res 2007 (Carcinoma, Squamous Cell) : To show that doxorubicin enhances the accumulation of IL-12 induced IFN gamma into tumors and the signal transducer and activator of transcription 1 ( Stat1 ) -dependent antitumor efficacy, the distribution of IFN gamma and the therapeutic end points, such as T-cell infiltration, inhibition of tumor vessel density, tumor growth inhibition, and inhibition of spontaneous tumor metastasis in wild-type and Stat1 ( -/- ) host and tumors were determined after the treatment at the indicated time points ... We discovered that doxorubicin enhances the accumulation of IL-12 induced IFN gamma in tumors
Figueiredo et al., Scand J Immunol 2007 (Uveitis) : To evaluate the role of IFN-gamma ( interferon gamma ) in IL-12- ( interleukin-12 ) -induced inhibition of the inflammatory response in the eye during endotoxin induced uveitis ( EIU )
Sá-Nunes et al., J Immunol 2007 : Saliva at remarkably diluted concentrations ( < 1/2000 ) promotes a dose dependent inhibition of IL-12 and TNF-alpha production induced by all TLR ligands used
Halder et al., J Clin Invest 2007 (Asthma...) : Using glycolipid/CD1d tetramers and cytokine responses, we showed that activation of sulfatide-reactive type II NKT cells and plasmacytoid DCs caused IL-12- and MIP-2 dependent recruitment of type I, or invariant, NKT ( iNKT ) cells into mouse livers
Shi et al., Arch Dermatol Res 2007 : We demonstrate that after treatment with PS, murine DCs display reduced expression of MHC II, CD80, CD86 and CD40 , but increased programmed death ligand-1 ( PD-L1 and PD-L2 ) ; and increased IL-10 and inhibited IL-12 cytokine production ... We demonstrate that after treatment with PS, murine DCs display reduced expression of MHC II, CD80 , CD86 and CD40, but increased programmed death ligand-1 ( PD-L1 and PD-L2 ) ; and increased IL-10 and inhibited IL-12 cytokine production ... We demonstrate that after treatment with PS, murine DCs display reduced expression of MHC II, CD80, CD86 and CD40, but increased programmed death ligand-1 ( PD-L1 and PD-L2 ) ; and increased IL-10 and inhibited IL-12 cytokine production ... We demonstrate that after treatment with PS, murine DCs display reduced expression of MHC II, CD80, CD86 and CD40, but increased programmed death ligand-1 ( PD-L1 and PD-L2 ) ; and increased IL-10 and inhibited IL-12 cytokine production
Sodhi et al., Glycoconj J 2007 : Pharmacological inhibitor of tyrosine kinase, PI3 kinase, protein kinase C, p42/44 , p38, JNK and intracellular calcium immobilizing agent down regulated the WGA induced expression of cytokines TNF-alpha, IL-1beta, IL-12 and IFN-gamma ... Pharmacological inhibitor of tyrosine kinase, PI3 kinase, protein kinase C, p42/44 , p38, JNK and intracellular calcium immobilizing agent down regulated the WGA induced expression of cytokines TNF-alpha, IL-1beta, IL-12 and IFN-gamma ... Pharmacological inhibitor of tyrosine kinase, PI3 kinase, protein kinase C, p42/44, p38 , JNK and intracellular calcium immobilizing agent down regulated the WGA induced expression of cytokines TNF-alpha, IL-1beta, IL-12 and IFN-gamma
Park et al., J Biochem Mol Biol 2007 (Dermatitis, Atopic) : CpG ODN treated DCs resulted in more production of IL-12
Hao et al., Wei Sheng Wu Xue Bao 2007 (Tuberculosis) : The results show that both ESAT-6 immunization and IL-12 + ESAT-6 combined immunization induce significantly anti-TB-PPD antibody production
Larocca et al., Int Immunopharmacol 2007 : VIP inhibited LPS induced TNF-alpha, IL-12 and nitrites accumulation in NOD macrophages while it increased IL-10 production ... VIP inhibited LPS induced TNF-alpha, IL-12 and nitrites accumulation in NOD macrophages while it increased IL-10 production
Moulton et al., J Leukoc Biol 2007 (Tuberculosis) : Complement C5a ( anaphylatoxin ) secreted by mycobacteria infected macrophages regulates IL-12p70 production
Boyle et al., Eur J Pharmacol 2007 : Considering that increased IL-10 production precedes suppression of IFN-gamma induced by MDMA, and also considering that IL-10 can inhibit IL-12 and IFN-gamma production, we examined the possibility that IL-10 was an essential mediator of the suppressive effect of MDMA on the IFN-gamma response
Chi et al., Chin J Cancer 2007 (Liver Neoplasms) : Interleukin-12 (IL-12) can promote the differentiation of Th1 cells and induce the production of interferon-gamma (IFN-gamma) by CD8+ T cells
Yamauchi et al., Int Immunol 2007 (Genetic Predisposition to Disease...) : Thus, we hypothesized that Ifi202 is involved in elevated IL-12 production from BWF1 BMDCs. Overexpression of Ifi202 enhanced the LPS induced IkappaB-zeta, IL-12p40 and NF-kappaB promoter activities, while anti-sense ( AS ) RNA against Ifi202 strongly suppressed them in a monocytic cell line, RAW 264.7 ... Thus, we hypothesized that Ifi202 is involved in elevated IL-12 production from BWF1 BMDCs. Overexpression of Ifi202 enhanced the LPS induced IkappaB-zeta, IL-12p40 and NF-kappaB promoter activities, while anti-sense ( AS ) RNA against Ifi202 strongly suppressed them in a monocytic cell line, RAW 264.7 ... Furthermore, overexpression of Ifi202 enhanced LPS induced IL-12p40 and IkappaB-zeta mRNA induction while Ifi202 AS RNA suppressed these in RAW 264.7 cells ... Furthermore, overexpression of Ifi202 enhanced LPS induced IL-12p40 and IkappaB-zeta mRNA induction while Ifi202 AS RNA suppressed these in RAW 264.7 cells
Tassi et al., Immunity 2007 : p110gamma played a major role in receptor induced interferon-gamma (IFN-gamma) production through a pathway that involved the kinase ERK and 5-Lipoxigenase, which most likely generates lipid mediators activating GPRCs. Conversely, PI3Ks negatively regulated interleukin-12 (IL-12) and IL-18 induced IFN-gamma by modulating p38 kinase activation ... p110gamma played a major role in receptor induced interferon-gamma (IFN-gamma) production through a pathway that involved the kinase ERK and 5-Lipoxigenase, which most likely generates lipid mediators activating GPRCs. Conversely, PI3Ks negatively regulated interleukin-12 (IL-12) and IL-18 induced IFN-gamma by modulating p38 kinase activation
Hu et al., Eur J Immunol 2007 (Listeriosis) : These cells rapidly secrete IFN-gamma upon stimulation with IL-12/IL-18 and are involved in innate responses to infection with Listeria monocytogenes
Hasan et al., Immunology 2008 : In this study we set out to investigate the potential role of Btk in Toll-like receptor 9 (TLR9) activation and the production of pro-inflammatory cytokines such as interleukin (IL)-6, tumour necrosis factor (TNF)-alpha and IL-12p40
Sohn et al., Vet Res 2007 : Bovine PMN were shown to secrete TNF-alpha, IL-1beta, IL-12 , IL-8 and IFN-gamma in response to LPS
Reddy et al., Cell Immunol 2007 : Modulation of CLA, IL-12R, CD40L, and IL-2Ralpha expression and inhibition of IL-12- and IL-23 induced cytokine secretion by CNTO 1275 ... CNTO 1275 inhibited upregulation of CLA, IL-12R , IL-2Ralpha and CD40L expression and also inhibited IL-12- and IL-23 induced IFN-gamma, IL-17A, tumor necrosis factor (TNF)-alpha , IL-2, and IL-10 secretion ... CNTO 1275 inhibited upregulation of CLA, IL-12R, IL-2Ralpha and CD40L expression and also inhibited IL-12- and IL-23 induced IFN-gamma, IL-17A, tumor necrosis factor (TNF)-alpha, IL-2, and IL-10 secretion ... CNTO 1275 inhibited upregulation of CLA, IL-12R , IL-2Ralpha and CD40L expression and also inhibited IL-12- and IL-23 induced IFN-gamma , IL-17A, tumor necrosis factor (TNF)-alpha, IL-2, and IL-10 secretion ... CNTO 1275 inhibited upregulation of CLA, IL-12R , IL-2Ralpha and CD40L expression and also inhibited IL-12- and IL-23 induced IFN-gamma, IL-17A, tumor necrosis factor (TNF)-alpha, IL-2, and IL-10 secretion ... CNTO 1275 inhibited upregulation of CLA, IL-12R , IL-2Ralpha and CD40L expression and also inhibited IL-12- and IL-23 induced IFN-gamma, IL-17A, tumor necrosis factor (TNF)-alpha, IL-2, and IL-10 secretion ... CNTO 1275 inhibited upregulation of CLA, IL-12R , IL-2Ralpha and CD40L expression and also inhibited IL-12- and IL-23 induced IFN-gamma, IL-17A , tumor necrosis factor (TNF)-alpha, IL-2, and IL-10 secretion ... CNTO 1275 inhibited upregulation of CLA, IL-12R, IL-2Ralpha and CD40L expression and also inhibited IL-12- and IL-23 induced IFN-gamma, IL-17A, tumor necrosis factor (TNF)-alpha, IL-2, and IL-10 secretion
Chang et al., Ann N Y Acad Sci 2007 (Inflammation) : While the expression of IFN-gamma in memory Th1 cells is independent of the original inducer IL-12, IL-12 is and remains required to induce expression of the regulatory cytokine IL-10 by Th1 memory cells giving IL-12 the potential to act anti-inflammatory during secondary responses ... While the expression of IFN-gamma in memory Th1 cells is independent of the original inducer IL-12 , IL-12 is and remains required to induce expression of the regulatory cytokine IL-10 by Th1 memory cells giving IL-12 the potential to act anti-inflammatory during secondary responses
Choi et al., FEMS Immunol Med Microbiol 2007 (Candidiasis) : These data indicate that CpG-ODN protects animals against lethal C. albicans challenge via a pathway that involves the TNF-alpha dependent induction of IL-12
Casas et al., Scand J Immunol 2007 (Hypersensitivity) : Stimulation with birch and LPS resulted in an increased secretion of IL-12p70 in samples from healthy when compared with that in allergic individuals
Waggoner et al., J Leukoc Biol 2007 : Herein, ligation of gC1qR on human monocyte derived DCs ( MDDCs ) with HCV core or anti-gC1qR agonist antibody was shown to inhibit TLR induced IL-12 production but not the production of other TLR stimulated cytokines ... Herein, ligation of gC1qR on human monocyte derived DCs ( MDDCs ) with HCV core or anti-gC1qR agonist antibody was shown to inhibit TLR induced IL-12 production but not the production of other TLR stimulated cytokines
Koch et al., Clin Immunol 2007 (Asthma) : Furthermore, the authors have studied the IL-12 induced expression of the T ( H ) 1-associated cytokine INF-gamma and the IL-4 induced expression of the T ( H ) 2-specific cytokine IL-5 in the presence of LPS using ELISA and compared nine atopic asthmatic subjects and eleven nonatopic normal volunteers ... Furthermore, the authors have studied the IL-12 induced expression of the T ( H ) 1-associated cytokine INF-gamma and the IL-4 induced expression of the T ( H ) 2-specific cytokine IL-5 in the presence of LPS using ELISA and compared nine atopic asthmatic subjects and eleven nonatopic normal volunteers ... Interestingly, LPS increased the IL-12 induced INF-gamma release in a concentration dependent manner in T-lymphocytes of normals but this could not be found in T cells of asthmatics, indicating an impaired T ( H ) 1 response to bacterial endotoxin LPS in asthma
Homma et al., J Interferon Cytokine Res 2007 : The Th2 transcription factor c-Maf inhibits IL-12p35 gene expression in activated macrophages by targeting NF-kappaB nuclear translocation ... The Th2 transcription factor c-Maf inhibits IL-12p35 gene expression in activated macrophages by targeting NF-kappaB nuclear translocation ... Regulation of IL-12 gene expression by the anti-inflammatory cytokine IL-10 represents a major homeostatic process underlying host-pathogen and host-self interactions ... When overexpressed, c-Maf can potently suppress IL-12 production ... This study contributes to our understanding of the molecular basis of the homeostatic regulation of IL-12 production by c-Maf , which plays a dual role both in the function of antigen presenting cells (APCs) and in T helper cell differentiation
Ochoa-Repáraz et al., Infect Immun 2007 (Q Fever...) : Cytokine analysis was performed on infected peritoneal macrophages isolated from these mice, and immunocompetent macrophages showed robust tumor necrosis factor alpha, IFN-gamma, and granulocyte-macrophage colony stimulating factor ( GM-CSF ) but no interleukin-12 (IL-12) responses ... Cytokine analysis was performed on infected peritoneal macrophages isolated from these mice, and immunocompetent macrophages showed robust tumor necrosis factor alpha, IFN-gamma, and granulocyte-macrophage colony stimulating factor ( GM-CSF ) but no interleukin-12 (IL-12) responses
Song et al., J Pharmacol Exp Ther 2008 (Chronic Disease...) : KRP-203 obviously inhibited the production of interferon-gamma, IL-12 , and tumor necrosis factor-alpha by the colonic lymphocytes, but had no influence on IL-4 production
Ohkuma et al., Clin Exp Immunol 2007 (Multiple Sclerosis, Relapsing-Remitting) : In addition, IgG treatment of cells from both healthy controls and MS patients inhibited the production of interleukin (IL)-12 , a cytokine associated with mDC differentiation, but did not inhibit the production of IL-10
Vidyarani et al., Cytokine 2007 (Tuberculosis, Pulmonary) : 1, 25 Dihydroxyvitamin D ( 3 ) significantly suppressed IL-12p40 and IFN-gamma production in response to CFA and live M. tuberculosis with a maximum suppression at 10 ( -7 ) M concentration ( p < 0.0001 )
Pintaric et al., Vet Immunol Immunopathol 2008 : Synergistic effects of IL-2, IL-12 and IL-18 on cytolytic activity, perforin expression and IFN-gamma production of porcine natural killer cells
Yang et al., Gastroenterology 2007 (Colitis) : In initial studies, we showed that splenocytes from NOD2 transgenic mice as compared with littermate controls exhibit decreased interleukin (IL)-12p70 responses to peptidoglycan ( PGN ), a TLR2 ligand that contains muramyl dipeptide, but not other TLR ligands ; in contrast, IL-12 responses to PAM ( 3 ) CSK ( 4 ), a TLR2 ligand that does not contain muramyl dipeptide, were normal
Barr et al., Eur J Immunol 2007 : In this study, we investigated which mouse B cell subsets are the most potent cytokine producers, and examined the role of Toll-like receptors ( TLR ) in the control of secretion of IL-6, IL-10, IL-12 and IFN-gamma by B cells
Tsujikawa et al., Proc Natl Acad Sci U S A 2007 (Hypertension...) : alphaCGRP and betaCGRP equally suppressed the production of TNF-alpha and IL-12 in bone marrow derived dendritic cells stimulated with lipopolysaccharide
Lin et al., FEBS Lett 2007 : Overexpression of GAK in activated T cells suppresses IL-12 induced IFN-gamma production but has no detectable effects on its proliferation, whereas knockdown of GAK by RNA interference ( RNAi ) increases IFN-gamma production ... Overexpression of GAK in activated T cells suppresses IL-12 induced IFN-gamma production but has no detectable effects on its proliferation, whereas knockdown of GAK by RNA interference ( RNAi ) increases IFN-gamma production
Ong'echa et al., Clin Immunol 2008 (Anemia...) : Since IL-23 and IL-12 share the IL-12p40 subunit and IL-12Rbeta1 receptor, and are down-regulated by IL-10 , relationships among these cytokines were explored in Kenyan children with varying severities of malarial anemia
Lalli et al., J Immunol 2007 : DAF regulated IL-12 production and subsequent T cell differentiation into IFN-gamma producing effectors was prevented by the deficiency of either C3 or C5a receptor (C5aR) in the APC, demonstrating a link between DAF, local complement activation, IL-12, and T cell produced IFN-gamma
Xiong et al., Sichuan Da Xue Xue Bao Yi Xue Ban 2007 : Bifidobacteria have effects on the differentiation, maturation and function of DC deriving from the monocyte : ( 1 ) The bifidobacterium can promote the maturation of DC morphology in vitro ; ( 2 ) the bifidobacterium can promote the function maturation of DC by enhancing the ability to stimulate the T lymphocyte proliferation ; ( 3 ) after the stimulation of the bifidobacterium, the level of IL-12 secreted by DC has been remarkably elevated, and the stimulated DC can promote the level of IFN-gamma secreted by T lymphocyte
Barkhuizen et al., J Infect Dis 2007 (Disease Models, Animal...) : Although there were similar plasma levels of immunoglobulin ( Ig ) M and IgG2a in IL-12-deficient mice and wild-type mice, interferon (IFN)- gamma production, especially during early infection, was severely impaired in all IL-12p70 ( -/- ) mouse strains, demonstrating an IL-12p70 dependent mechanism for IFN- gamma production ... Because IFN- gamma receptor-deficient mice ( IFN- gamma R ( -/- ) ) were also highly susceptible to both Trypanosoma species, IL-12p70 dependent IFN- gamma production seems to be the important mechanism involved in resistance against both pathogens
Mandron et al., J Invest Dermatol 2008 (Dermatitis, Atopic...) : Monocytes from AD patients ( 15 adults with mostly severe disease and 15 children with mild to moderate disease ) exhibited an activated and tolerant state as supported by ( i ) secretion of large amounts of IL-6, IL-10, and tumor necrosis factor-alpha even in the absence of stimulation ; ( ii ) their inability to modulate neither HLA-DR and CD54 nor TLR2 and TLR4 expression after in vitro challenge with SEB ; ( iii ) inhibition of IL-12p70 secretion in response to LPS
Bose et al., Hum Immunol 2007 (Carcinoma, Squamous Cell...) : Overall the results suggest that NLP effects NK cellular cytotoxicity by CD40-CD40L mediated endogenous production of IL-12 , which critically controls perforin dependent tumor cell cytotoxicity ... Overall the results suggest that NLP effects NK cellular cytotoxicity by CD40-CD40L mediated endogenous production of IL-12 , which critically controls perforin dependent tumor cell cytotoxicity
Roda et al., Clin Cancer Res 2007 : Stimulation of human NK cells with cetuximab coated tumor cells and IL-2, IL-12 , or IL-21 resulted in 3-fold to 10-fold higher IFN-gamma production than was observed with either agent alone
Lapteva et al., Cancer Res 2007 : Whereas neither iCD40 nor TLR-4 signaling alone led to high levels of interleukin (IL)-12p70 and IL-6, using iCD40 in combination with lipopolysaccharide (LPS) or monophosphoryl lipid A led to strongly synergistic production of both
Fahey et al., J Cell Mol Med 2007 (Multiple Sclerosis) : We report that curcumin decreases IL-12 induced STAT4 phosphorylation, IFN-gamma production, and IL-12 Rbeta1 and beta2 expression ... We report that curcumin decreases IL-12 induced STAT4 phosphorylation, IFN-gamma production, and IL-12 Rbeta1 and beta2 expression ... We report that curcumin decreases IL-12 induced STAT4 phosphorylation, IFN-gamma production, and IL-12 Rbeta1 and beta2 expression
Sugiyama et al., Int Immunol 2008 : IL-12p40 was also produced by poly ( A : U ) -stimulated pDC in a TLR7 dependent manner
Torvinen et al., Respir Res 2007 : Previous reports have shown increased levels of IFN-gamma, IFN-gamma inducing IL-12 and IFN-gamma-inducible chemokine IP-10 in patients with chronic obstructive pulmonary disease ( COPD ) ... Furthermore, inhibition studies supported the suggestion that different intracellular pathways are responsible of IFN-gamma and IL-12 mediated IP-10 secretion
Vasquez et al., Infect Immun 2008 (Leishmaniasis) : We found that CXCL10 induced IL-12 p40 production but reduced IL-10 production in uninfected DCs
Hsu et al., Transpl Immunol 2007 : DCs treated with suplatast expressed lower levels of CD40, CD80, and CD86 and reduced IL-12 production
Dey et al., Scand J Immunol 2007 (Leishmaniasis, Visceral) : These chemokines induced the known pro-inflammatory cytokine interleukin (IL)-12 secretion and inhibited the secretion of anti-inflammatory cytokines IL-10 and transforming growth factor-beta in infected macrophages
Wysocka et al., Clin Lymphoma Myeloma 2007 (Lymphoma, T-Cell, Cutaneous...) : Patients ' peripheral blood mononuclear cells ( PBMCs ) stimulated with the TLR7 agonist 3M-001 produced high levels of interferon ( IFN ) -alpha , and the TLR8 agonist 3M-002 potently induced predominantly interleukin (IL)-12 and IFN-gamma
Liese et al., Eur J Immunol 2007 (Leishmaniasis, Cutaneous) : Here, we show that TLR9 was required for the induction of IL-12 in bone marrow derived DC by intact L. major parasites or L. major DNA and for the early IFN-gamma expression and cytotoxicity of NK cells following infection with L. major in vivo
Pang et al., Cell Immunol 2007 (Colitis, Ulcerative) : Increased expression and activation of IL-12 induced Stat4 signaling in the mucosa of ulcerative colitis patients
Segawa et al., Int J Food Microbiol 2008 : Some L. brevis strains, which were selected for their ability to induce a strong Th1 immune response, inhibited both total immunoglobulin E ( IgE ) and antigen specific IgE production, and improved the Th1/Th2 balance by enhancing IL-12 and IFN-gamma and inhibiting IL-4 production from ovalbumin ( OVA ) -sensitized mouse splenocytes
Wen et al., Blood 2008 : Specifically, IL-12 expression was regulated by stable reciprocal changes in histone H3 lysine-4 trimethylation ( H3K4me3 ) and histone H3 lysine-27 dimethylation ( H3K27me2 ), as well as changes in cognate histone methyltransferase (HMT) complexes on the Il12p35 and Il12p40 promoters
Kano et al., Nat Immunol 2008 : Notably, the IRF1 dependent induction of IL-12Rbeta1 was essential for IFN-gamma-IL-12 signaling but was dispensable for IL-23-IL-17 signaling ... Notably, the IRF1 dependent induction of IL-12Rbeta1 was essential for IFN-gamma-IL-12 signaling but was dispensable for IL-23-IL-17 signaling
Hashimoto et al., Int Immunopharmacol 2008 (Neoplasms, Experimental) : OK-TX-ppt also induced tumor necrosis factor (TNF)-alpha, IL-10, IL-12 , and interferon (IFN)-gamma in PBMC ... OK-TX-ppt also increased the levels of IL-12 and IFN-gamma in mouse spleen cells in vitro
Cheng et al., Int Immunopharmacol 2008 : The production of IL-1 was induced by GP at a dose of 10 microg/mL ; but, NO, IL-6 and IL-12 was significantly induced at 100 microg/mL
Thomas et al., Infect Immun 2008 (Leishmaniasis, Cutaneous) : We also found IL-10 dependent ( major ) and -independent ( minor ) inhibition of IL-12 mediated by FcgammaRIII, as well as parasite mediated inhibition of IL-12 and induction of IL-10, independent of FcgammaR ... We also found IL-10 dependent ( major ) and -independent ( minor ) inhibition of IL-12 mediated by FcgammaRIII , as well as parasite mediated inhibition of IL-12 and induction of IL-10, independent of FcgammaR
Ichikawa et al., Biosci Biotechnol Biochem 2007 : MyD88 but not TLR2 , 4 or 9 is essential for IL-12 induction by lactic acid bacteria ... MyD88 but not TLR2, 4 or 9 is essential for IL-12 induction by lactic acid bacteria
Wang et al., Chin J Cancer 2007 (Neovascularization, Pathologic...) : IL-12 can promote IFN-gamma to induce IP-10 production, thereafter, inhibit neovascularization in tumors
de Witte et al., Immunobiology 2007 : VLP interaction with DCs resulted in the up-regulation of co-stimulatory molecules and the production of the cytokines IL-6, IL-8, IL-10 and IL-12p40 ... VLP interaction with DCs resulted in the up-regulation of co-stimulatory molecules and the production of the cytokines IL-6, IL-8, IL-10 and IL-12p40
Siegemund et al., Immunobiology 2007 : Stimulation of BMDC and BMMPhi with S. enterica serovar Enteritidis ( SE ) or LPS resulted in the release of IL-12 and IL-23 by BMDC but not by BMMPhi ... Using BMDC originating from wild-type ( wt ), TLR2 ( def ) and TLR4 ( def ) mice, we show that in BMDC the induction of IL-12 , IL-23, and IL-27p28 by SE is dependent on TLR4 , whereas low-level production of p40 is also mediated by pattern recognition receptors ( PRR ) other than TLR4
Young et al., Blood 2008 : Continuous ligation of LILRB1 modulated cellular differentiation, conferred a unique phenotype upon the resultant cells, induced a profound resistance to CD95 mediated cell death, and inhibited secretion of cytokines IL-10, IL-12p70 , and TGF-beta
Kondadasula et al., Blood 2008 : Studies with specific chemical inhibitors revealed that the activation of ERK was dependent on the activation of PI3-K, whose activation was dependent on Syk, and that sequential activation of these molecules was required for NK cell IFN-gamma production in response to FcR and IL-12 stimulation ... Retroviral transfection of ERK1 into primary human NK cells substantially increased IFN-gamma production in response to immobilized IgG and IL-12 , while transfection of human NK cells with a dominant negative ERK1 abrogated IFN-gamma production ... Retroviral transfection of ERK1 into primary human NK cells substantially increased IFN-gamma production in response to immobilized IgG and IL-12 , while transfection of human NK cells with a dominant negative ERK1 abrogated IFN-gamma production
Tripathi et al., Int Immunol 2008 : AG490, inhibitor of JAK2, down-regulated transcription factors c-jun and STAT1 and inhibited the PRL induced IFN-gamma, TNF-alpha, IL-1 beta and IL-12p40 production in macrophages
Moons et al., Carcinogenesis 2008 (Barrett Esophagus...) : The IL-10 ( -1082 ) GG genotype, which is associated with higher IL-10 levels, was also associated with a decreased risk of BE when it was associated with the IL-12B C-allele, indicating IL-10 dependent down-regulation of IL-12p70 expression
Mättö et al., Scand J Immunol 2008 : BCR stimulation also stabilized and enhanced the IFN-gamma mRNA production induced by IL-12 and IL-18
Margenthaler et al., Hepatology research : the official journal of the Japan Society of Hepatology 2008 : Conclusion : IL-12 regulated IFN-gamma production is crucial during the priming phase by P. acnes, but not at the time of the subsequent LPS challenge
Siracusa et al., J Immunol 2008 : Exposure of bone marrow derived dendritic cells to E2 also enhanced production of IL-12 in response to the TLR ligands, CpG and LPS ... CD11c ( + ) splenocytes isolated from animals with supplemental E2 produced more IFN-gamma in response to IL-12 and IL-18 ... CD11c ( + ) splenocytes isolated from animals with supplemental E2 produced more IFN-gamma in response to IL-12 and IL-18
Frasca et al., J Immunol 2008 : Administered in association with classical maturation stimuli such as CD40 or TLR-4 stimulation, IFN-gamma up-regulated IL-27 and IL-12 production, CCR7-driven migration, and activated Th-1 cell recruitment, whereas it decreased IL-10 production and STAT3 phosphorylation
Zhao et al., J Huazhong Univ Sci Technolog Med Sci 2007 : Effect of TGF-beta1 on the expression of IL-12 , IL-15, IL-18, IL-4 and IL-10 in heart transplantation rejection in rats
Selvaraj et al., J Clin Immunol 2008 (Genetic Predisposition to Disease...) : The extended genotype bbaaTT ( baT haplotype ) was associated with decreased IL-12p40 and IFN-gamma levels and significantly increased IL-10 levels ( p < 0.05 ) ... We studied the influence of VDR promoter ( Cdx-2 and A1012G ), 3 ' untranslated region ( Apa I, Bsm I, and Taq I ) and start codon ( Fok I ) polymorphisms on 1,25 ( OH ) ( 2 ) D ( 3 ) -modulated IL-12p40 , IFN-gamma, IL-10, and IL-5 response to live Mycobacterium tuberculosis and its culture filtrate antigen (CFA) in 60 normal healthy subjects and 51 pulmonary tuberculosis patients
Tsukamoto et al., J Immunol 2008 : In the p110beta-deficient cells, inducible NO synthase expression and IL-12 production upon stimulation by LPS were increased, whereas LPS induced expression of COX-2 and activation of MAPKs were unaffected
Osterloh et al., Int Immunol 2008 : LPS-free Hsp60 did not induce IL-6, IL-12 or tumor necrosis factor alpha production in APC nor were these cytokines needed for Hsp60 mediated T cell co-stimulation in the absence of LPS
Page et al., J Leukoc Biol 2008 : Here, we demonstrate involvement of PKCtheta in IL-12 induced NK cell IFN-gamma production ... NK cells from PKCtheta ( -/- ) mice produced less IFN-gamma in response to IL-12 than those from wild-type ( WT ) mice ... IL-12 induced NK cell cytotoxicity was unaffected, and NK cells from PKCtheta ( -/- ) mice did not display reduced IFN-gamma production in response to IL-18, indicating a specific role for PKCtheta in IL-12 induced IFN-gamma production ... Under the conditions tested, T cells did not produce IFN-gamma in response to IL-12 or affect the ability of NK cells to produce the cytokine ... Transcription of IFN-gamma increased similarly in NK cells from WT and PKCtheta ( -/- ) mice in response to IL-12 , and there was no difference in IFN-gamma mRNA stability ... Taken together, these findings indicate a role for PKCtheta in the post-transcriptional regulation of IL-12 induced IFN-gamma production
Taneichi et al., Clin Immunol 2008 (Agammaglobulinemia...) : Stimulation with TLR2, TLR4 and TLR7/8 ligands, as well as TLR3 ligand, resulted in significantly lower production of TNF-alpha, but neither IL-6 nor IL-12p70 , by DCs from XLA patients in comparison to normal controls ... Stimulation with TLR2 , TLR4 and TLR7/8 ligands, as well as TLR3 ligand, resulted in significantly lower production of TNF-alpha, but neither IL-6 nor IL-12p70 , by DCs from XLA patients in comparison to normal controls
Kekarainen et al., J Gen Virol 2008 (Porcine Postweaning Multisystemic Wasting Syndrome...) : Notably, PCV2 induced IL-10 led to effective repression of IL-12 in blood peripheral mononuclear cells ( PBMCs )
Lee et al., J Biol Chem 2008 : The addition of exogenous recombinant IL-10 could suppress IL-12 production by TLR9 activated Btk ( -/- ) B cells, suggesting that in B cells, Btk negatively regulates IL-12 through the induction of autocrine IL-10 production ... Thus, Btk plays an important role in TLR9 signaling and acts separately to regulate NFkappaB RelA activation as well as IL-10 and IL-12 production in B cells
Maglione et al., J Immunol 2008 (Autoimmune Diseases...) : These data strongly suggest that FcgammaRIIB engagement can dampen the TB Th1 response by attenuating IL-12p40 production or activation of APCs
Flohé et al., Mol Med 2008 (Sepsis) : Endogenous IL-10 was not responsible for the impaired IL-12 secretion
Jayakumar et al., Infect Immun 2008 : The inhibition of NF-kappaB activation by the pretreatment of DC with caffeic acid phenethyl ester blocks L. major induced IRF-1 and IRF-8 activation and IL-12 expression
Jarnicki et al., J Immunol 2008 (MAP Kinase Signaling System...) : Inhibition of p38 suppressed TLR induced IL-10 and PGE ( 2 ) and enhanced IL-12 production in DC
Athié-Morales et al., J Immunol 2008 : Although IL-12 costimulation leads to a greater IFN-gamma response by NK cells, higher levels of CD69 in response to MDP are induced in the presence of IFN-alpha, suggesting that different pathogen induced cytokine profiles will affect downstream NK cell responses ... Although IL-12 costimulation leads to a greater IFN-gamma response by NK cells, higher levels of CD69 in response to MDP are induced in the presence of IFN-alpha, suggesting that different pathogen induced cytokine profiles will affect downstream NK cell responses
Johnson et al., Dev Comp Immunol 2008 : Bovine WC1 ( - ) gammadeltaT cells incubated with IL-15 express the natural cytotoxicity receptor CD335 ( NKp46 ) and produce IFN-gamma in response to exogenous IL-12 and IL-18 ... In addition, CD335 ( + ) gammadeltaT cells demonstrated a robust ability to produce IFN-gamma in response to exogenous IL-12 and IL-18
Shao et al., J Leukoc Biol 2008 (Fibrosis) : IL-4, IL-13, and IFN-gamma act directly on monocytes to regulate fibrocyte differentiation, and IL-12 acts indirectly, possibly through CD16 positive NK cells ... IL-4, IL-13 , and IFN-gamma act directly on monocytes to regulate fibrocyte differentiation, and IL-12 acts indirectly, possibly through CD16 positive NK cells ... IL-4 , IL-13, and IFN-gamma act directly on monocytes to regulate fibrocyte differentiation, and IL-12 acts indirectly, possibly through CD16 positive NK cells
Liu et al., Eur J Immunol 2008 : The CD4 subset stimulated by alpha-galactosylceramide ( alpha-GalCer ) -loaded DC immediately produced massive amounts of IL-4 and IL-13, which together with IFN-gamma enhanced CD40L induced IL-12 production by DC ... The CD4 subset stimulated by alpha-galactosylceramide ( alpha-GalCer ) -loaded DC immediately produced massive amounts of IL-4 and IL-13, which together with IFN-gamma enhanced CD40L induced IL-12 production by DC
Dar et al., Vet Immunol Immunopathol 2008 : In contrast, in LNC, only IL-12 was stimulated by all three classes of CpG ODNs, while IFNalpha, and IL-6 were induced by A- and C-class ODNs
Jayakumar et al., J Parasitol 2008 (Leishmaniasis) : Using a luciferase reporter assay assessing IL-12 transcription, we report here that Leishmania major, Leishmania donovani, and Leishmania chagasi inhibit IL-12 transcription in response to interferon-gamma, lipopolysaccharide, and CD40 ligand and that Leishmania spp. lipophosphoglycan, phosphoglycans, and major surface protein are not necessary for inhibition ... Using a luciferase reporter assay assessing IL-12 transcription, we report here that Leishmania major, Leishmania donovani, and Leishmania chagasi inhibit IL-12 transcription in response to interferon-gamma , lipopolysaccharide, and CD40 ligand and that Leishmania spp. lipophosphoglycan, phosphoglycans, and major surface protein are not necessary for inhibition
Nishimura et al., J Parasitol 2008 : These results suggest that the WS inhibits IL-12p40 mRNA production by promoting production of macrophage colony stimulating factor by macrophages
Jones et al., Immunology 2008 (Leishmaniasis, Visceral...) : In contrast, LPS induced interleukin-12 (IL-12) production could be downregulated by all three steroids, indicating that ligation by progesterone of either the glucocorticoid or the progesterone receptors or both could mediate this effect
Tao et al., J Invest Dermatol 2008 (Melanoma...) : Therefore, TAP expression restores both antigen presentation and immunogenicity in A375 melanoma cells and concomitantly increases IL-12 and IFN-gamma production in tumor antigen-specific CTLs ; TAP should be considered as a part of the immunotherapies for MM
Arruvito et al., J Immunol 2008 : The expression of PR had a functional counterpart in the suppressive effect of P4 on IL-12 induced IFN-gamma secretion
Lee et al., Immunology 2008 : We found that de novo expression or overexpression of OX40L, 4-1BBL and IL-12p70 on activated B cells synergistically enhanced proliferation as well as IL-2 and interferon-gamma production by CD8+ T cells ... We found that de novo expression or overexpression of OX40L, 4-1BBL and IL-12p70 on activated B cells synergistically enhanced proliferation as well as IL-2 and interferon-gamma production by CD8+ T cells
Hickey et al., J Leukoc Biol 2008 : Adenylate cycalse toxin of Bordetella pertussis inhibits TLR induced IRF-1 and IRF-8 activation and IL-12 production and enhances IL-10 through MAPK activation in dendritic cells
Wilson et al., J Immunol 2008 (Toxoplasmosis, Animal) : Our results underscore a vital role for IL-12 in not only the induction of IFN-gamma expression but also in the development of heterogeneous subpopulations of effector CD8 ( + ) T cells generated in response to the intracellular parasite T. gondii
Lamhamedi-Cherradi et al., J Immunol 2008 (Respiratory Hypersensitivity) : Exogenous IL-12p70 added to cocultures of DCs and T cells resulted in reduced IL-4 and increased IFN-gamma expression when DCs were activated with AP ... Exogenous IL-12p70 added to cocultures of DCs and T cells resulted in reduced IL-4 and increased IFN-gamma expression when DCs were activated with AP
Mutwiri et al., Vaccine 2008 : Incubation of mouse splenocytes with PCEP or PCPP ( in the absence of antigen ) stimulated production of IL-4 and IL-12 , but only PCEP induced significant IFNgamma production
Trepiakas et al., Vaccine 2008 (Neoplasms) : This DC maturation cocktail was described to fulfill the criteria for optimal DC generation and to be superior to the standard DC ( sDC ) cocktail as it induced fully mature DCs with potent IL-12p70 secretion together with CCR7 expression which is necessary for priming of a TH1 response and for migration to the draining lymph node, respectively
Simpson-Abelson et al., J Immunol 2008 (Carcinoma, Non-Small-Cell Lung...) : The effector memory T cells that had been shown to be quiescent in human lung tumor microenvironments can be activated in situ as determined by the production of human IFN-gamma in response to exogenous IL-12
Steers et al., Vaccine 2008 : L ( LA ) enhanced the surface expression of MHC class I, class II, CD80 , and CD86 molecules, induced the secretion of IFN-gamma, IL-12p40 , TNF-alpha and IL-10, and caused a shift in the proteasome profile from constitutive to immunoproteasomes as observed by the induction of beta2i, beta5i, PA28alpha, and PA28beta subunits ... L ( LA ) enhanced the surface expression of MHC class I, class II, CD80, and CD86 molecules, induced the secretion of IFN-gamma, IL-12p40 , TNF-alpha and IL-10, and caused a shift in the proteasome profile from constitutive to immunoproteasomes as observed by the induction of beta2i, beta5i, PA28alpha, and PA28beta subunits
Kawana et al., Infect Immun 2008 : Ligation of surface expressed CD1d by monoclonal antibody cross linking promoted interleukin-12 (IL-12) and IL-15, but not IL-10, production in vaginal and penile urethral cells
Romagnani et al., Arthritis Res Ther 2008 (Autoimmune Diseases) : Moreover, the presence in the circulation and in various tissues of Th cells that can produce both IL-17 and interferon-gamma , as well as the flexibility of human Th17 clones to produce interferon-gamma in addition to IL-17 in response to IL-12 , suggests that there may be a developmental relationship between Th17 and Th1 cells, at least in humans ... Moreover, the presence in the circulation and in various tissues of Th cells that can produce both IL-17 and interferon-gamma, as well as the flexibility of human Th17 clones to produce interferon-gamma in addition to IL-17 in response to IL-12 , suggests that there may be a developmental relationship between Th17 and Th1 cells, at least in humans
Yu et al., Biochem Pharmacol 2008 : Like quercetin inhibits macrophage induced cytokine production, it also blocks IL-12 dependent JAK-STAT signaling in Th cells ... Like quercetin inhibits macrophage induced cytokine production, it also blocks IL-12 dependent JAK-STAT signaling in Th cells
Jackson et al., Int J Cancer 2008 (Melanoma...) : Melanoma-lysate markedly suppressed TLR4 dependent IL-12p40 and p70 production
Ni et al., J Invest Dermatol 2008 (Sezary Syndrome...) : We found that IFN-gamma was required for IL-12p70 production by monocyte derived DCs from SzS
Gerosa et al., J Exp Med 2008 : DC priming with interferon (IFN) gamma strongly increased IL-12 production, but was not required for IL-23 production and inhibited IL-23 production induced by beta-glucan
Kim et al., J Immunol 2008 (Neoplasms, Experimental) : IL-12p40 is a natural antagonist which inhibits IL-12- and IL-23 mediated biological activity by blocking the binding of IL-12/23 to their receptors
Xu et al., Int J Tuberc Lung Dis 2008 : Our study demonstrated that after stimulation of alveolar macrophages by Mce4E protein for 48 h, the expression of TNF-alpha was induced , with an enhanced IL-6 mRNA level ( P < 0.05 ) and no changes in IL-12 expression in the macrophages
Ohtani et al., Blood 2008 (Leishmaniasis, Cutaneous) : Phosphoinositide 3-kinase (PI3K) negatively regulates Toll-like receptor ( TLR ) -mediated interleukin-12 (IL-12) expression in dendritic cells (DCs) ... Rapamycin, an inhibitor of mTOR, enhanced IL-12 production in LPS stimulated DCs, whereas the activation of mTOR by lentivirus mediated transduction of a constitutively active form of Rheb suppressed the production of IL-12
Dobreva et al., Cytokine 2008 (MAP Kinase Signaling System) : The inhibition of both JNK and p38 augmented IL-12p40 production
Henjakovic et al., Toxicol Appl Pharmacol 2008 : MALP-2 only failed to alter cytokine or chemokine levels, but was highly effective in combination with IFNgamma resulting in increased levels of TNFalpha, IL-12 ( p40 ), RANTES, and IL-1alpha
Argueta-Donohué et al., Exp Parasitol 2008 : Leishmania mexicana : participation of NF-kappaB in the differential production of IL-12 in dendritic cells and monocytes induced by lipophosphoglycan ( LPG )
Bednarska et al., Exp Parasitol 2008 (Cryptosporidiosis...) : During infection with C. parvum there was an increase in production of IFN-gamma and IL-12 but co-infection with H. bakeri inhibited IFN-gamma secretion
Calippe et al., J Immunol 2008 : In striking contrast, we show that chronic administration of E2 to ovariectomized mice markedly increases the expression of IL-1beta, IL-6, IL-12p40 , and inducible NO synthase by resident peritoneal macrophages in response to LPS ex vivo
Bouguermouh et al., J Immunol 2008 (Dermatitis, Contact) : Although selective CD47 deficiency on DCs led to increased IL-12p70 production, CD47 ( -/- ) Tg T cells produced more IFN-gamma and displayed higher T-bet expression than CD47 ( +/+ ) Tg T cells in response to OVA loaded CD47 ( -/- ) DCs
Wang et al., J Immunol 2008 (Chronic Disease...) : The NF-kappaB subunit c-Rel can regulate expression of IL-12/23 subunits suggesting that it could have a critical role in mediating the development of chronic inflammation within the lower bowel
Pakpour et al., J Immunol 2008 (Leishmaniasis, Cutaneous) : In this study, we show that the Leishmania-specific central memory CD4 ( + ) T cells require IL-12 to produce IFN-gamma, demonstrating that this population needs additional signals to develop into Th1 cells ... In this study, we show that the Leishmania-specific central memory CD4 ( + ) T cells require IL-12 to produce IFN-gamma , demonstrating that this population needs additional signals to develop into Th1 cells
Xin et al., Mol Immunol 2008 (Leishmaniasis, Cutaneous) : La amastigote infected DCs were less mature and less potent antigen presenting cells (APC) than their promastigote infected counterparts, as judged by the lower expression of CD40 and CD83, suppressed cytokine expression ( IL-12p40 and IL-10 ), reduced effectiveness for priming CD4+ T cells from naïve or infected mice
Robinson et al., J Infect Dis 2008 (Tuberculosis) : Quantitative measurement of transcripts showed that IL-12 or M. tuberculosis induced IL-27 gene expression in human macrophages
Kasahara et al., J Immunol 2008 (Acute Disease...) : However, exposure to acrolein after LPS instillation markedly diminished the LPS induced production of several inflammatory cytokines, specifically TNF-alpha, IL-12 , and the Th1 cytokine IFN-gamma, which was associated with reduction in NF-kappaB activation
Prescott et al., J Allergy Clin Immunol 2008 (Hypersensitivity...) : Maternal allergy ( n = 59 ) was associated with significantly higher neonatal IL-12 and IFN-gamma responses to TLR2, TLR3 , and TLR4 activation, whereas TNF-alpha and IL-6 responses to TLR2, TLR4, and TLR5 activation were significantly higher in newborns who subsequently had allergic disease ( n = 32 ) ... Maternal allergy ( n = 59 ) was associated with significantly higher neonatal IL-12 and IFN-gamma responses to TLR2, TLR3, and TLR4 activation, whereas TNF-alpha and IL-6 responses to TLR2, TLR4, and TLR5 activation were significantly higher in newborns who subsequently had allergic disease ( n = 32 )
Dasgupta et al., Hybridoma (Larchmt) 2008 : On the other hand, antibodies produced from clones a3-3a and a3-7g specifically recognized p40 and inhibited p40- but not p40 ( 2 ) -, IL-12- , and IL-23 induced production of inflammatory molecules and activation of NF-kappaB
Yammani et al., Virology 2008 : Importantly, the control of IL-12 production was differentially dependent on MyD88 signaling
Takedatsu et al., Gastroenterology 2008 (Chronic Disease...) : Furthermore, IFN-gamma and IL-17 production from CD4 ( + ) T cells, induced by IL-12 and IL-23 respectively, was enhanced synergistically by combination with TL1A ... Furthermore, IFN-gamma and IL-17 production from CD4 ( + ) T cells, induced by IL-12 and IL-23 respectively, was enhanced synergistically by combination with TL1A
Iborra et al., Microbes Infect 2008 (Leishmaniasis, Cutaneous) : This protection was associated with the induction of an IL-12 dependent specific-IFN-gamma response mediated mainly by CD4 ( + ) T cell, albeit a minor contribution of CD8 ( + ) T cells can not be ruled out ... This protection was associated with the induction of an IL-12 dependent specific-IFN-gamma response mediated mainly by CD4 ( + ) T cell, albeit a minor contribution of CD8 ( + ) T cells can not be ruled out
Aldhous et al., Inflamm Bowel Dis 2008 (Colitis, Ulcerative...) : Stimulation by LPS and PHA ( 5 microg/mL ) increased IL12/IL23p40 production from CD and UC versus HC ( P < 0.05 ) ; PHA ( 0.5 microg/mL ) increased IL1beta in UC and decreased TGFbeta from CD and UC ( P < 0.01 )
Zhang et al., Hum Immunol 2008 : In the present study, we explored the effect of IL-12 upregulation on the NK receptor NKG2D and on the promotion of NK cell function ... IL-12 enhanced the cytotoxicity of NK cells to different solid and hematological tumor cell lines and promoted interferon-gamma secretion by NK cells ... Also, IL-12 augmented the expression of cytotoxic effector molecules, TRAIL and perforin, and the phosphorylation of STAT1 , STAT4, and ERK1/2, which may also contribute to lysis by NK cells ... Also, IL-12 augmented the expression of cytotoxic effector molecules, TRAIL and perforin, and the phosphorylation of STAT1, STAT4 , and ERK1/2, which may also contribute to lysis by NK cells ... Also, IL-12 augmented the expression of cytotoxic effector molecules, TRAIL and perforin, and the phosphorylation of STAT1, STAT4, and ERK1/2, which may also contribute to lysis by NK cells ... Also, IL-12 augmented the expression of cytotoxic effector molecules, TRAIL and perforin, and the phosphorylation of STAT1, STAT4, and ERK1/2 , which may also contribute to lysis by NK cells
Koizumi et al., Immunol Lett 2008 : S-PT84 induced IFN-gamma producing cells through activation of IL-12 production by CD11c ( + ) DCs in Toll-like receptor (TLR) 2- and/or TLR4 dependent manner ... S-PT84 induced IFN-gamma producing cells through activation of IL-12 production by CD11c ( + ) DCs in Toll-like receptor (TLR) 2- and/or TLR4 dependent manner ... S-PT84 induced IFN-gamma producing cells through activation of IL-12 production by CD11c ( + ) DCs in Toll-like receptor (TLR) 2- and/or TLR4 dependent manner
Randhawa et al., Cell Microbiol 2008 : We found that absence of CD43 resulted in reduced production of TNF-alpha, IL-12 and IL-6 by M. tuberculosis infected macrophages
Lombardi et al., Clin Exp Allergy 2008 (Asthma...) : Among the three TLR2 ligands tested, the synthetic lipopeptide Pam3CSK4 is the most potent inducer of IL-12p35 and IL-10 gene expression in murine bone marrow derived DCs, as well as in purified oral myeloid DCs ... Among the three TLR2 ligands tested, the synthetic lipopeptide Pam3CSK4 is the most potent inducer of IL-12p35 and IL-10 gene expression in murine bone marrow derived DCs, as well as in purified oral myeloid DCs
Zhang et al., J Hepatol 2008 (Disease Progression...) : In particular, activated hepatic pDCs produced interferon ( IFN ) -alpha , which subsequently induced interleukin (IL)-12 and IL-10 production via toll-like receptor-9 ligation in liver infiltrating lymphocytes cultured in vitro
Motegi et al., J Leukoc Biol 2008 (Bacterial Infections...) : We previously proposed that mouse CD8 ( + ) CD122 ( + ) T cells and human CD57 ( + ) T cells, which increase with age and exhibit potent IFN-gamma production, represent a double edged sword as they play critical roles in host defense and the lethal IL-12/LPS induced generalized Shwartzman reaction (GSR)
Price et al., Immunology 2009 (Tuberculosis, Bovine) : Reciprocal enhancement of IL-12 secretion by the DCs was also observed and this interaction was found to be contact dependent
Dauer et al., J Immunol Methods 2008 : These `` conventional '' FastDC are mature, migrate in response to CCR7 ligation and effectively stimulate autologeous T cells in vitro, but are deficient in IL-12p70 production
Carrieri et al., Immunopharmacol Immunotoxicol 2008 (Multiple Sclerosis, Relapsing-Remitting) : We examined the effects of interferon (IFN)beta-1a on interleukin (IL)-12p70 and IL-10 secretion in 27 Relapsing Remitting Multiple Sclerosis ( RRMS ) patients, divided in responders and non-responders
El Ghaoui et al., Phytother Res 2008 : MSE and SLE appeared to have no effect on anti-EA antibody production, but enhanced IL-12 and gamma-interferon gene transcription
Arai et al., Allergol Int 2008 (Hypothermia) : This augmented IFN-gamma production was caused by the up-regulation of IL-12 production from CD14+ blood monocytes
Filén et al., Mol Cell Proteomics 2009 : Besides T cell receptor signaling, IL-12 induced STAT4 and T-bet- and IL-4 induced STAT6 and GATA3 signaling pathways are the major players regulating the Th1 and Th2 differentiation process, respectively ... Besides T cell receptor signaling, IL-12 induced STAT4 and T-bet- and IL-4 induced STAT6 and GATA3 signaling pathways are the major players regulating the Th1 and Th2 differentiation process, respectively ... Besides T cell receptor signaling, IL-12 induced STAT4 and T-bet- and IL-4 induced STAT6 and GATA3 signaling pathways are the major players regulating the Th1 and Th2 differentiation process, respectively
Behrens et al., J Immunol 2008 : A number of recent studies show that activation of CR3 on dendritic cells (DCs) suppresses TLR induced TNF-alpha and IL-12 production and inhibits effective Ag presentation ... TLR induced TNF-alpha and IL-12 production by bone marrow derived DCs occurs heterogeneously, with apoptotic cells inhibiting only certain populations depending on the TLR agonist ... In contrast, although apoptotic cells induced homogeneous IL-10 production by DCs, IL-10 was not necessary for the inhibition of TNF-alpha and IL-12
Chiu et al., J Immunol 2008 (Inflammation) : This restoration was reversed by IL-12 neutralization, indicating that IL-10 suppressed sources of IL-12 in aged mice
Qi et al., Sci China C Life Sci 2000 : Taken together, the data suggest : ( i ) IFN-gamma/LPS could strongly induce the expression of IL-12 p40 and p35 mRNA ; both the expression levels were equal ; this phenomenon coincided with the high-level secretion of IL-12 p70 induced by IFN-gamma/LPS ; ( ii ) NF-kappaB signal pathway is essential for IFN-gamma/LPS to induce IL-12 mRNA expression ; ( iii ) by blocking the degradation of IkappaB, the PSI suppresses the IL-12 p40/p35 mRNA expression induced by LPS and IFN-gamma/LPS ; ( iv ) NF-kappaB signal may not be involved in the mechanism by which IFN-gamma enhanced the expression of the LPS induced IL-12 p40/p35 mRNA ... Taken together, the data suggest : ( i ) IFN-gamma/LPS could strongly induce the expression of IL-12 p40 and p35 mRNA ; both the expression levels were equal ; this phenomenon coincided with the high-level secretion of IL-12 p70 induced by IFN-gamma/LPS ; ( ii ) NF-kappaB signal pathway is essential for IFN-gamma/LPS to induce IL-12 mRNA expression ; ( iii ) by blocking the degradation of IkappaB, the PSI suppresses the IL-12 p40/p35 mRNA expression induced by LPS and IFN-gamma/LPS ; ( iv ) NF-kappaB signal may not be involved in the mechanism by which IFN-gamma enhanced the expression of the LPS induced IL-12 p40/p35 mRNA
Enríquez-de-Salamanca et al., Cytokine 2008 (Conjunctivitis, Allergic...) : TNF-alpha stimulated secretion of IL-12p40 was significantly increased by 30 min ; GM-CSF, MCP-1 , IL-6, IL-7, IL-8, and RANTES were significantly increased by 2 h, and IFN-gamma and IL-1alpha by 24 h ... TNF-alpha stimulated secretion of IL-12p40 was significantly increased by 30 min ; GM-CSF, MCP-1, IL-6, IL-7, IL-8, and RANTES were significantly increased by 2 h, and IFN-gamma and IL-1alpha by 24 h ... TNF-alpha stimulated secretion of IL-12p40 was significantly increased by 30 min ; GM-CSF , MCP-1, IL-6, IL-7, IL-8, and RANTES were significantly increased by 2 h, and IFN-gamma and IL-1alpha by 24 h ... TNF-alpha stimulated secretion of IL-12p40 was significantly increased by 30 min ; GM-CSF, MCP-1, IL-6, IL-7, IL-8, and RANTES were significantly increased by 2 h, and IFN-gamma and IL-1alpha by 24 h ... TNF-alpha stimulated secretion of IL-12p40 was significantly increased by 30 min ; GM-CSF, MCP-1, IL-6, IL-7, IL-8, and RANTES were significantly increased by 2 h, and IFN-gamma and IL-1alpha by 24 h ... TNF-alpha stimulated secretion of IL-12p40 was significantly increased by 30 min ; GM-CSF, MCP-1, IL-6 , IL-7, IL-8, and RANTES were significantly increased by 2 h, and IFN-gamma and IL-1alpha by 24 h ... TNF-alpha stimulated secretion of IL-12p40 was significantly increased by 30 min ; GM-CSF, MCP-1, IL-6, IL-7 , IL-8, and RANTES were significantly increased by 2 h, and IFN-gamma and IL-1alpha by 24 h ... TNF-alpha stimulated secretion of IL-12p40 was significantly increased by 30 min ; GM-CSF, MCP-1, IL-6, IL-7, IL-8 , and RANTES were significantly increased by 2 h, and IFN-gamma and IL-1alpha by 24 h
Trotta et al., J Immunol 2008 : Likewise, NK cells obtained from smad3 ( -/- ) mice produced more IFN-gamma in response to CD16 activation plus IL-12 when compared with NK cells obtained from wild-type mice
Jiang et al., J Immunol 2008 : Hematopoietic progenitor cells differentiated in the presence of CD137 protein display enhanced phagocytic activity, secrete high levels of IL-10 but little IL-12 in response to LPS , and are incapable of stimulating T cell proliferation
Ishida et al., J Immunol 2008 (Disease Models, Animal...) : Furthermore, CX3CL1 enhanced the gene expression of IL-1beta, TNF-alpha, IFN-gamma, and IL-12 by WT macrophages with NF-kappaB activation
Chandra et al., Clin Exp Immunol 2008 (Leishmaniasis, Visceral...) : In addition parasite structural integrity but not viability was required for suppression of TLR2 stimulated IL-12p40 and activation of IL-10
Wang et al., Blood 2008 (MAP Kinase Signaling System) : IL-12 and IL-18 stimulated autoreactively activated NKT cells to secrete IFN-gamma, and this was mediated by Janus kinase-signal transducers and activators of transcription ( JAK-STAT ) -dependent signaling without induction of calcium flux ... IL-12 and IL-18 stimulated autoreactively activated NKT cells to secrete IFN-gamma, and this was mediated by Janus kinase-signal transducers and activators of transcription ( JAK-STAT ) -dependent signaling without induction of calcium flux
Buza et al., Vet Immunol Immunopathol 2008 : Depletion of CD14+ cells from PBMC abrogated the IL-12 response and consequently the IFN-gamma response , suggesting that CD14+ cells are required for PBMC immune activation with ORN
Del Prete et al., J Allergy Clin Immunol 2008 (Disease Models, Animal...) : This study provides evidence that HP-NAP enhances endogenous IL-12 and IFN-gamma response and exerts a powerful anti-T ( H ) 2 activity in vivo, targeting both IL-5 induced eosinophilia and IL-4 mediated hyper-IgE responses induced by parasitic infection
Stolte et al., Mol Immunol 2008 : Cortisol administered at stress levels to phagocytes in vitro significantly inhibits LPS induced expression of the pro-inflammatory cytokines tumor necrosis factor alpha (TNF-alpha) and interleukin-12 (IL-12) ( subunit p35 ) and of inducible nitric oxide synthase (iNOS) expression
Latvala et al., World J Gastroenterol 2008 : S. thermophilus induced the expression of pro-inflammatory ( TNF-alpha, IL-12 , IL-6, and CCL20 ) and Th1 type ( IL-12 and IFN-gamma ) cytokines, while B. breve and L. lactis were also potent inducers of anti-inflammatory IL-10
Barkhuizen et al., J Infect Dis 2008 (Parasitemia...) : In contrast, IL-10 production was not affected by the absence of IL-12 ... Taken together, these results show that, during T. congolense infection, the absence of IL-12, but not the IL-12p80 homodimer or IL-23, leads to a reduction in IFN-gamma production, which reduces hepatic pathology and improves host survival in conjunction with IL-10 without negatively affecting parasitemia control
Su et al., Nan Fang Yi Ke Da Xue Xue Bao 2008 : In the NBD-Peptide-DC treated group, the serum levels of IL-12 and IFN-gamma decreased significantly ( P < 0.01 ), but the levels of IL-4 and IL-10 increased significantly ( P < 0.01 ) ... In the NBD-Peptide-DC treated group, the serum levels of IL-12 and IFN-gamma decreased significantly ( P < 0.01 ), but the levels of IL-4 and IL-10 increased significantly ( P < 0.01 )
Lee et al., Korean J Parasitol 2008 (Inflammation) : Co-treatment of THP-1 cells with T. gondii lysate inhibited the LPS induced IL-12 , IL-8 and TNF-alpha expression, but increased the level of IL-10 synergistically
Goulaouic et al., J Immunotoxicol 2008 (Inflammation) : PAH induced significant secretion of IL-1beta, IL-8, and IL-12 after 24 or 48 hr of treatment, an effect reinforced by LPS stimulation ; no effect on IL-10 secretion was noted ... Moreover, PAH coating on ufCB tended to minimize the effect of LPS stimulation ; this included ( i ) inhibition of the decrease in IL-12 secretion induced by uncoated ufCB and ( ii ) stimulation of IL-10 production
Barkman et al., Microbes Infect 2008 : Here the bacterial structures and signalling pathways involved were studied and compared with those leading to IL-6 production, and to IL-12 production in response to LPS after IFN-gamma pre-treatment ... Intact bifidobacteria induced massive production of IL-12 ( 1 ng/ml ) and IL-6 ( > 30 ng/ml ) from human PBMC, whereas fragmented bifidobacteria induced IL-6 , but no IL-12 ... LPS induced moderate levels of IL-12 ( 0.31 ng/ml ), but only from IFN-gamma pre treated PBMC
Robinson et al., J Immunol 2008 (Plague) : Yersinia pestis evades TLR4 dependent induction of IL-12 ( p40 ) 2 by dendritic cells and subsequent cell migration
Mo et al., J Immunol 2008 (Encephalomyelitis, Autoimmune, Experimental...) : Signal transducer and activator of transcription 4 ( Stat4 ) is a transcription factor activated by IL-12 and IL-23, two cytokines known to play important roles in the pathogenesis of EAE by inducing T cells to secrete IFN-gamma and IL-17, respectively ... Signal transducer and activator of transcription 4 ( Stat4 ) is a transcription factor activated by IL-12 and IL-23, two cytokines known to play important roles in the pathogenesis of EAE by inducing T cells to secrete IFN-gamma and IL-17 , respectively
Wang et al., Eur J Immunol 2008 (Acquired Immunodeficiency Syndrome...) : In contrast, B7-H1 ligation with soluble programmed death-1 (PD-1) reduced mDC maturation and IL-12 production but increased mDC apoptosis and IL-10 production
Tcherepanova et al., BMC molecular biology 2008 : Ectopic expression of a truncated CD40L protein from synthetic post-transcriptionally capped RNA in dendritic cells induces high levels of IL-12 secretion
Tian et al., Zhongguo Shi Yan Xue Ye Xue Za Zhi 2008 (Leukemia, Promyelocytic, Acute) : The results indicated that in experiment groups, the cells had dendritic appearance and cytogenetic characteristics of APL ; expression of CD1a, CD83, CD80, CD86, HLA-DR and CD1d as well as level of IL-12 obviously increased ; the MLR and CTL effects were significant, but increase of CD1a expression in HL60-DCs did not show statistical difference from control and blank control groups ... The results indicated that in experiment groups, the cells had dendritic appearance and cytogenetic characteristics of APL ; expression of CD1a, CD83, CD80, CD86, HLA-DR and CD1d as well as level of IL-12 obviously increased ; the MLR and CTL effects were significant, but increase of CD1a expression in HL60-DCs did not show statistical difference from control and blank control groups ... The results indicated that in experiment groups, the cells had dendritic appearance and cytogenetic characteristics of APL ; expression of CD1a, CD83, CD80, CD86, HLA-DR and CD1d as well as level of IL-12 obviously increased ; the MLR and CTL effects were significant, but increase of CD1a expression in HL60-DCs did not show statistical difference from control and blank control groups
Sasaki et al., Eur J Immunol 2008 (Melanoma, Experimental) : Notably, the inhibitory effects of IL-4 on Tc1 expression of VLA-4 could be blocked by the presence of IL-12 , but not IFN-gamma ... Notably, the inhibitory effects of IL-4 on Tc1 expression of VLA-4 could be blocked by the presence of IL-12 , but not IFN-gamma
Hu et al., Immunity 2008 : TLR- and Notch induced Hes1 and Hey1 attenuated IL-6 and IL-12 production ... TLR- and Notch induced Hes1 and Hey1 attenuated IL-6 and IL-12 production
Kawasaki et al., Am J Physiol Cell Physiol 2009 (Disease Models, Animal...) : Moreover, IL-15 enhanced IL-12 induced IFN-gamma production and antigen presentation by splenic DCs
Karpuzoglu et al., Endocrinology 2009 : The activation of STAT4beta was mediated by IL-12 and not IFNgamma because deliberate addition or neutralization of IL-12, but not IFNgamma, affected the activation of STAT4beta ... In contrast to IL-12 induced activation of STAT4beta in cells from estrogen treated mice, STAT4alpha was not increased, rather it tended to be decreased
Slobodin et al., Immunol Invest 2008 (Raynaud Disease...) : The production of ET-1 by MDDCs was negligible in all examined conditions, while the release of TNFalpha and IL-12 was stimulated by LPS but not by ET-1
Hibi et al., Cytotechnology 2003 : MLN derived CD11c ( + ) /B220 ( - ) DCs induce higher IFN-gamma production from naive CD4 ( + ) T cells than SPL derived DCs, through efficient IL-12 secretion ... MLN derived CD11c ( + ) /B220 ( - ) DCs induce higher IFN-gamma production from naive CD4 ( + ) T cells than SPL derived DCs, through efficient IL-12 secretion
Marshall et al., J Immunol 2008 (MAP Kinase Signaling System) : During this process, cells initiate proteolytic degradation of internalized protein Ags into peptides that complex with MHC class II (MHC II) and simultaneously increase expression of costimulatory molecules and of cytokines such as IL-6, IL-12 , and IL-23
Schulz et al., J Immunol 2008 (Necrosis...) : To analyze the role of IL-23 in the absence of IL-12 , low doses of S. Enteritidis were administered to p35 ( -/- ) mice ( lacking IL-12 ), p35/19 ( -/- ) mice ( lacking IL-12 and IL-23 ), p35/40 ( -/- ) mice ( lacking IL-12, IL-23, and homodimeric IL-12p40 ), or p35/IL-17A ( -/- ) mice ( lacking IL-12 and IL-17A )
Campos Alberto et al., Immunology 2009 : TNF-alpha but not IL-12 increases the expression of IL-2Rbeta on CB Vgamma9 T cells
Ma et al., J Biol Chem 2009 (Acquired Immunodeficiency Syndrome) : HIV-1 Nef inhibits lipopolysaccharide induced IL-12p40 expression by inhibiting JNK activated NFkappaB in human monocytic cells ... We have previously shown that lipopolysaccharide (LPS) induced IL-12p40 production in monocytic cells is regulated by NFkappaB and AP-1 transcription factors through the activation of two distinct upstream signaling pathways, namely the c-Jun-N-terminal kinase (JNK) and the calmodulin dependent protein kinase-II activated pathways ... We have previously shown that lipopolysaccharide (LPS) induced IL-12p40 production in monocytic cells is regulated by NFkappaB and AP-1 transcription factors through the activation of two distinct upstream signaling pathways, namely the c-Jun-N-terminal kinase (JNK) and the calmodulin dependent protein kinase-II activated pathways ... We have previously shown that lipopolysaccharide (LPS) induced IL-12p40 production in monocytic cells is regulated by NFkappaB and AP-1 transcription factors through the activation of two distinct upstream signaling pathways, namely the c-Jun-N-terminal kinase (JNK) and the calmodulin dependent protein kinase-II activated pathways ... Herein, we show that intracellular nef expressed through transduction of primary monocytes and promonocytic THP-1 cells with retroviral mediated nef gene inhibited LPS induced IL-12p40 transcription by inhibiting the JNK mitogen activated protein kinases without affecting the calmodulin dependent protein kinase-II activated pathway
Sommer et al., Vet Immunol Immunopathol 2009 : In contrast, MDM cells infected with MAP failed to up-regulate expression of iNOS and IL-12p40 genes in response to CD40L ... In uninfected cells, inhibition of MAPK revealed that CD40L mediated increase in IL-6 gene expression was dependent on activation of ERK1/2, while increases in IL-12p40 , iNOS, and IL-10 gene expression were dependent on activation of p38
Gruenbaum et al., Biochem Pharmacol 2009 : Furthermore, BIRB796 and compound 2 inhibited the production of TNF-alpha in THP-1 monocytes and the IL-12/IL-18 induced production of interferon-gamma in human T-cells with similar potencies
Lifshitz et al., Mol Immunol 2009 : In-vitro stimulation of these DCs with recombinant human EPO enhanced viability, upregulated CD80, CD86 and MHC class II and augmented the secretion of IL-12 ... In-vitro stimulation of these DCs with recombinant human EPO enhanced viability, upregulated CD80, CD86 and MHC class II and augmented the secretion of IL-12
Onari et al., Clin Exp Allergy 2009 (Asthma...) : The results suggest that endogenous IL-12p40 is essential for inhibition of AHR and peribronchial fibrosis, but not eosinophilic inflammation, in a murine asthma model with prolonged antigen exposures
Lin et al., J Virol 2009 : Binding of CD40L to its receptor upregulates expression of major histocompatibility complex class I, class II, and costimulatory molecules on DCs and increases production of proinflammatory cytokines and chemokines, especially interleukin 12 (IL-12)
Davis et al., J Immunol 2008 : Although naive cells rapidly secrete IL-2 in response to Ag stimulation, IL-12 inhibits IL-2 secretion in daughter cells as they differentiate into Th1 cells
Henry et al., J Immunol 2008 : IL-12 produced by dendritic cells augments CD8+ T cell activation through the production of the chemokines CCL1 and CCL17 ... Therefore, we sought to determine the individual roles of IL-12 and IL-23 in naive CD8 ( + ) T cell activation by addressing their ability to influence IFN-gamma production and cellular interaction dynamics during priming by Listeria monocytogenes infected dendritic cells ( DC )
Balkow et al., Exp Dermatol 2009 : When compared with untreated DC or DC treated with anti-CD40 alone, DC matured with anti-CD40 plus IL-12 and IL-18 expressed significantly more IFN-gamma and IL-12 , induced enhanced CD8 ( + ) T-cell proliferation, prolonged synaptic interaction with T cells and increased CD8 ( + ) T-cell mediated cytotoxicity
Canda-Sánchez et al., J Membr Biol 2009 : IL-12 also enhances the interaction between CD26 and CD45RO, which removes the phosphatase CD45RO from raft microdomains ... IL-12 also enhances the interaction between CD26 and CD45RO , which removes the phosphatase CD45RO from raft microdomains
Hensler et al., J Gen Virol 2009 : We detected expression of interleukin (IL)-6, tumour necrosis factor (TNF)-alpha, macrophage inflammatory protein (MIP)-1alpha, MIP-1beta, RANTES and IL-12p40 from 2 to 6 h post-infection, and these peaked by 15-24 h. Expression of these factors decreased 24-48 h post-infection, with the exception of TNF-alpha which remained high throughout the entire 72 h. Interestingly, while IL-12p40 expression increased post-infection, bioactive IL-12p70 was not detected in the supernatants ... We detected expression of interleukin (IL)-6, tumour necrosis factor (TNF)-alpha, macrophage inflammatory protein (MIP)-1alpha, MIP-1beta, RANTES and IL-12p40 from 2 to 6 h post-infection, and these peaked by 15-24 h. Expression of these factors decreased 24-48 h post-infection, with the exception of TNF-alpha which remained high throughout the entire 72 h. Interestingly, while IL-12p40 expression increased post-infection, bioactive IL-12p70 was not detected in the supernatants
Sharabi et al., PLoS Biol 2008 : Moreover, Twist-2 was found to have a contrasting effect on cytokine production : inhibiting the production of proinflammatory cytokines such as interleukin-12 (IL-12) and interferon-gamma (IFNgamma) while promoting the regulatory cytokine IL-10 by myeloid cells
Wang et al., Zhongguo Shi Yan Xue Ye Xue Za Zhi 2008 : Compared with neu-Fc or GM-CSF or BCG treatment alone, neu-Fc in combination with GM-CSF and BCG significantly stimulated IL-12 production and inhibited IL-10 production ( p < 0.01 )
Liu et al., Zhonghua Er Ke Za Zhi 2008 : ( 3 ) As compared with the control mice, concentration of IL-12p70 induced by LPS and IL-10 induced by BCG in vitro from spleen cells culture supernatant was noticeably elevated ( P < 0.05 ) in neonatal BCG treated BALB/c mice, but that of IL-6 did not change by LPS or BCG stimulation ... ( 3 ) As compared with the control mice, concentration of IL-12p70 induced by LPS and IL-10 induced by BCG in vitro from spleen cells culture supernatant was noticeably elevated ( P < 0.05 ) in neonatal BCG treated BALB/c mice, but that of IL-6 did not change by LPS or BCG stimulation ... ( 1 ) By up-regulating splenic CD8alpha ( + ) DCs and inducing IL-12p70 and IL-10 production in BALB/c mice, neonatal BCG vaccination promoted Th1/Tr1 development
Jana et al., Mol Immunol 2009 (Multiple Sclerosis) : IL-12 p40 homodimer, but not IL-12 p70, induces the expression of IL-16 in microglia and macrophages
Tierney et al., Immunol Cell Biol 2009 (Encephalomyelitis, Autoimmune, Experimental) : Our results show that lower doses of interferon-gamma (IFN-gamma) were more effective at priming bone marrow derived macrophages ( BMMphi ) to produce more interleukin 10 (IL-10) and less IL-12p40 in response to lipopolysaccharide (LPS) and immune complexes compared with LPS alone
Crettaz et al., J Virol 2009 (Hepatitis B...) : These studies showed that lymphocytes from healthy woodchucks or from animals with low viremia levels produced gamma interferon ( IFN-gamma ) upon IL-12 stimulation, while lymphocytes from woodchucks with high viremia failed to upregulate IFN-gamma in response to IL-12
Rigaux et al., Allergy 2009 (Hypersensitivity) : IL-12 p40 secretion was dependent on nuclear factor-kappaB (NF-kappaB) , mitogen activated protein ( MAP ) kinases, Toll-like receptor 2 (TLR2), TLR9 and on the bacterial teichoic acid composition
Hayashi et al., J Dairy Res 2009 (Dermatitis, Atopic) : As a result, it became clear that IL-12 induced by strain T120 increased production of IFN-gamma and total IgE production was mainly controlled by the IFN-gamma
Charoensap et al., Trans R Soc Trop Med Hyg 2008 (Melioidosis) : We also found that, although the LPS mutant could readily activate monocyte derived human DCs , it induced lower levels of IL-12p70 and IL-10 production than its wild-type counterpart ( P < 0.01 ) ... We also found that, although the LPS mutant could readily activate monocyte derived human DCs, it induced lower levels of IL-12p70 and IL-10 production than its wild-type counterpart ( P < 0.01 )
Pertyńska-Marczewska et al., Am J Reprod Immunol 2009 (Diabetes Mellitus, Type 1...) : LPS stimulated IL-12 was positively correlated with levels of AGEs in the third trimester
Lyakh et al., Immunol Rev 2008 : Mycobacterium tuberculosis and yeast zymosan induce IL-23 , but in the absence of other stimuli, no IL-12 is induced in human dendritic cells (DCs)
Cooper et al., Immunol Rev 2008 (Tuberculosis) : We also show that IL-12p70 is required for the optimal interferon-gamma (IFN-gamma) T-cell response, which is required for control of Mtb growth ... IL-23 can induce IFN-gamma responses in the lung if IL-12 is not present, but its major role is in supporting the IL-17 response within the lung ... IL-23 can induce IFN-gamma responses in the lung if IL-12 is not present, but its major role is in supporting the IL-17 response within the lung
Larangé et al., J Leukoc Biol 2009 : TLR7 and TLR8 activation up-regulated CCR7, CD40, CD86, and CD83 expression and IL-6 and IL-12p40 production ... However, only TLR8 activation led to IL-12p70 production and il-12p35 mRNA expression
Mäkelä et al., J Leukoc Biol 2009 (MAP Kinase Signaling System) : DNA binding assays showed that TLR3 and TLR8 stimulation induced binding of multiple IFN regulatory factor ( IRF ) and STAT transcription factors to the IL-12p35 gene promoter IFN stimulated response element in moDCs and macrophages but with different binding profiles and kinetics ... DNA binding assays showed that TLR3 and TLR8 stimulation induced binding of multiple IFN regulatory factor ( IRF ) and STAT transcription factors to the IL-12p35 gene promoter IFN stimulated response element in moDCs and macrophages but with different binding profiles and kinetics
Baruah et al., Blood 2009 : Furthermore, human DCs from 2 C1q-deficient patients were found to have impaired IL-12p70 production in response to CD40L stimulation ... Our novel data suggest that C1q augments the production of IL-12p70 by mouse and human DCs after CD40 triggering and plays important roles in sustaining the maturation of DCs and guiding the activation of T cells
Takeshima et al., Infect Immun 2009 (Gastritis...) : Therefore, we investigated IL-12 p40 mRNA induction by H. pylori in gastric epithelial cells and T cells
Avni et al., Immunol Lett 2009 (Inflammation) : In this study we thus investigated the effect of PCERA-1 on LPS induced release of TNFalpha, interleukin (IL)-10 and IL-12p40 , in vivo, and ex vivo
Stewart et al., Cancer Cell 2009 (Neoplasms) : In this issue of Cancer Cell, Kortylewski et al. show that in the tumor microenvironment, STAT3 enhances the expression of the protumor cytokine IL-23 in macrophages but inhibits the antitumor cytokine IL-12 in dendritic cells ... In this issue of Cancer Cell, Kortylewski et al. show that in the tumor microenvironment, STAT3 enhances the expression of the protumor cytokine IL-23 in macrophages but inhibits the antitumor cytokine IL-12 in dendritic cells ... In this issue of Cancer Cell, Kortylewski et al. show that in the tumor microenvironment, STAT3 enhances the expression of the protumor cytokine IL-23 in macrophages but inhibits the antitumor cytokine IL-12 in dendritic cells
Kortylewski et al., Cancer Cell 2009 (Neoplasms) : Furthermore, Stat3 inhibits NF-kappaB/c-Rel dependent IL-12/p35 gene expression in tumor associated dendritic cells ... Furthermore, Stat3 inhibits NF-kappaB/c-Rel dependent IL-12/p35 gene expression in tumor associated dendritic cells ... Furthermore, Stat3 inhibits NF-kappaB/c-Rel dependent IL-12/p35 gene expression in tumor associated dendritic cells
Romagnani et al., Mol Immunol 2009 : Second, human Th17 cells express T-bet in addition to retinoic acid related orphan receptor ( ROR ) gammat and can be induced to produce IFN-gamma in addition to IL-17A in the presence of IL-12 , thus suggesting a close developmental relationship with Th1 cells
Kadoshima-Yamaoka et al., Immunol Lett 2009 : ASB16165, a novel inhibitor for phosphodiesterase 7A (PDE7A), suppresses IL-12 induced IFN-gamma production by mouse activated T lymphocytes ... ASB16165, which has an IC50 value of 15 nM for human PDE7A, suppressed IL-12 induced IFN-gamma production by T lymphoblasts which have been prepared by stimulating mouse T cells with anti-CD3 antibody ... Forskolin ( an adenylyl cyclase activator ) and dibutyryl cAMP also inhibited the IL-12 induced IFN-gamma synthesis
Alcón et al., Eur J Immunol 2009 : NK cells expressing high levels of CD72, upon stimulation with IL-12 and IL-18 or target cells, produce significantly less IFN-gamma than those expressing low levels of CD72, whereas both subsets are equally cytotoxic
Cui et al., Vaccine 2009 (Listeriosis) : Furthermore, IFNgamma induced by CpG was required in vivo for optimal production of IL-12 , which in turn, influenced effector CD8 T cell longevity
Bode et al., J Immunol 2009 : Kinetic of RelA activation controls magnitude of TLR mediated IL-12p40 induction ... TLR ligands induce IL-12 to differing extents
Kobayashi et al., J Immunol 2009 (Asthma) : Importantly, Alternaria inhibited IL-12 production by activated DCs, and DCs exposed to Alternaria enhanced Th2 polarization of CD4 ( + ) T cells
Canda-Sánchez et al., Immunobiology 2009 : IL-12 dependent activation of ERK1/2 in human T lymphoblasts ... We have previously shown that interleukin-12 (IL-12) increases the expression of CD26, promoting the interaction of CD26 with CD45R0 ( a CD45 isoform ) and removing CD45R0 from lipid rafts ... We have previously shown that interleukin-12 (IL-12) increases the expression of CD26, promoting the interaction of CD26 with CD45R0 ( a CD45 isoform ) and removing CD45R0 from lipid rafts ... Thus, this IL-12 dependent removal of CD45RO from rafts could, hypothetically, fulfill functions like the activation of the ERK1/2 pathway ... However, even though c-Myc is slightly up-regulated by IL-12 and partially mediates the proliferative effect of IL-12, this transcription factor was not found downstream of ERK1/2
Franke et al., Scand Cardiovasc J 2009 : We therefore assessed the effect of IL-12 on IFN-gamma-synthesis and pro-inflammatory cytokine release in vitro before and after cardiac surgery ... IL-12 increases IFN-gamma-synthesis before and after surgery without influencing pro-inflammatory cytokine synthesis
Colić et al., Mol Immunol 2009 (Inflammation...) : IL-23 stimulated the production of IL-17 by PL-MNC, whereas the secretion of IFN-gamma was enhanced by both IL-12 and IL-23
Ge et al., Leuk Res 2009 (Leukemia, Myeloid, Acute) : We further demonstrated that a significant decrease in IL-12 production, increase in IL-10 production by DLLC, and an increased CD4 ( + ) CD25 ( + ) Foxp3 ( + ) T regulatory population lead to the defective T cell immune response that is induced by B7-H1 up-regulation on DLLC
Perona-Wright et al., J Immunol 2009 (Encephalomyelitis, Autoimmune, Experimental) : Engagement of CD40 on P. acnes conditioned DC stimulated the release of IL-12 , IL-23, and IL-6, of which IL-6 alone proved essential for Th17 differentiation
Jung et al., J Immunol 2009 (Disease Models, Animal...) : In vitro and in vivo, IDO expression was increased by exogenous IL-12 , but decreased by exogenous IL-10 in dendritic cells and splenic dendritic cells ... Interestingly, whereas LPS induced IL-12 levels in serum were higher than those of IL-10, the balance between serum IL-12 and IL-10 following challenge became reversed in IDO ( -/- ) - or 1-methyl-D-tryptophan treated mice
Joo et al., PloS one 2009 (Colitis...) : Moreover, LPS induced IL-12p40 gene expression was dose-dependently inhibited in TLE treated splenocytes
Shanmugarajan et al., J Cell Biochem 2009 : Whether, IL-12 regulates OIP-1 expression in the bone microenvironment is unclear ... Because IL-12 induces IFN-gamma production by T cells, we tested whether IFN-gamma participates in IL-12 stimulation of OIP-1 gene expression in these cells ... IL-12 treatment in the presence of IFN-gamma neutralizing antibody significantly increased OIP-1 mRNA expression, suggesting that IL-12 directly regulates OIP-1 gene expression ... Interestingly, real-time PCR analysis demonstrated that IL-12 induces OIP-1 expression ( 3.2-fold ) in CD4+ T cells ; however, there was no significant change in CD8+ T cells ... Chromatin immunoprecipitation ( ChIP ) assay confirmed STAT-3, but not STAT-1 binding to the OIP-1 gene promoter in response to IL-12 stimulation ... Chromatin immunoprecipitation ( ChIP ) assay confirmed STAT-3 , but not STAT-1 binding to the OIP-1 gene promoter in response to IL-12 stimulation
Wang et al., J Immunol 2009 : However, ESAT-6 also contributes to virulence in animal models, mediates cellular cytolysis, and inhibits IL-12 production by mononuclear phagocytes
Gozalbo et al., Frontiers in bioscience (Landmark edition) 2009 (Candidiasis) : IFN-gamma is produced by CD4 Th1, CD8 , gamma delta T, and natural killer ( NK ) cells, essentially in response to both IL-12 and/or IL-18, and plays an important role in the regulation of the immune system as well as in the control of the infectious process ... IFN-gamma is produced by CD4 Th1, CD8, gamma delta T, and natural killer ( NK ) cells, essentially in response to both IL-12 and/or IL-18, and plays an important role in the regulation of the immune system as well as in the control of the infectious process
Huang et al., Infect Immun 2009 : YGPs stimulated secretion of the proinflammatory cytokine tumor necrosis factor alpha (TNF-alpha) in wild-type murine bone marrow derived myeloid dendritic cells ( BMDCs ) but did not stimulate interleukin-12p70 (IL-12p70) production ... The upregulation of TNF-alpha and downregulation of IL-12p70 required Dectin-1 , but not IL-10 ... The upregulation of TNF-alpha and downregulation of IL-12p70 required Dectin-1, but not IL-10
Gee et al., Inflamm Allergy Drug Targets 2009 (Autoimmune Diseases...) : IL-12 is required for the induction of IFN-gamma production, critical for the induction of Th1 cells
Koga et al., Immunity 2009 : Overexpression of c-Fos suppressed LPS induced cytokine production, whereas cAMP mediated suppression of TNF-alpha and IL-12 was impaired in Fos ( -/- ) DCs or in RAW264.7 cells treated with c-Fos siRNA
Lemos et al., Proc Natl Acad Sci U S A 2009 (Arthritis, Rheumatoid...) : Methylated BSA ( mBSA ) and IL-23 induced neutrophil migration was inhibited by anti-IL-23 and anti-IL-17 antibodies, COX inhibitors, IL-12 , or IFNgamma but was enhanced by prostaglandin E ( 2 ) ( PGE ( 2 ) ) ... IL-17 induced neutrophil migration was not affected by COX inhibitors, IL-12 , or IFNgamma but was inhibited by MK886 ( a leukotriene synthesis inhibitor ), anti-TNFalpha, anti-CXCL1, and anti-CXCL5 antibodies and by repertaxin ( a CXCR1/2 antagonist ) ... Our results suggest that prostaglandin plays an important role in IL-23 induced neutrophil migration in arthritis by enhancing IL-17 synthesis and by inhibiting IL-12 and IFNgamma production ... Our results suggest that prostaglandin plays an important role in IL-23 induced neutrophil migration in arthritis by enhancing IL-17 synthesis and by inhibiting IL-12 and IFNgamma production
Kang et al., Cytokine 2009 : Induction of IL-12 by LMM in peritoneal macrophages involved NF-kappaB, p38 and JNK, but not ERK1/2 ... Induction of IL-12 by LMM in peritoneal macrophages involved NF-kappaB, p38 and JNK, but not ERK1/2 ... Induction of IL-12 by LMM in peritoneal macrophages involved NF-kappaB , p38 and JNK, but not ERK1/2
Richter et al., J Immunol 2009 : Ncf1 provides a reactive oxygen species independent negative feedback regulation of TLR9 induced IL-12p70 in murine dendritic cells ... Because only CpG/TLR9 induced IL-12p70 was regulated by Ncf1, we used TRIF ( -/- ) and MyD88 ( -/- ) cells to show that TLR9/MyD88 was primarily affected ... Because only CpG/TLR9 induced IL-12p70 was regulated by Ncf1 , we used TRIF ( -/- ) and MyD88 ( -/- ) cells to show that TLR9/MyD88 was primarily affected ... Finally, proving the in vivo relevance CpG adjuvant guided OVA immunization resulted in a strong augmentation of IL-12p70 dependent Th1 IFN-gamma response only in Ncf1-deficient mice
Morrow et al., Blood 2009 : Moreover, we describe for the first time how IL-28B reduces regulatory T-cell populations during DNA vaccination, whereas IL-12 increases this cellular subset
Jana et al., Glia 2009 (MAP Kinase Signaling System) : IL-12 p40 homodimer, the so-called biologically inactive molecule, induces nitric oxide synthase in microglia via IL-12R beta 1 ... Earlier we have demonstrated that IL-12 p40 homodimer ( p40 ( 2 ) ) induces the expression of inducible nitric oxide synthase (iNOS) in microglia ... This study was undertaken to investigate underlying mechanisms required for IL-12 p40 ( 2 ) - and IL-12 p70 induced expression of iNOS in microglia ... This study was undertaken to investigate underlying mechanisms required for IL-12 p40 ( 2 ) - and IL-12 p70 induced expression of iNOS in microglia ... In contrast, both IL-12R beta 1 and IL-12R beta 2 were involved for IL-12 p70 induced microglial expression of iNOS
Matsushita et al., Nature 2009 (Anemia...) : Macrophages from Zc3h12a ( -/- ) mice showed highly increased production of interleukin (IL)-6 and IL-12p40 ( also known as IL12b ), but not TNF, in response to TLR ligands
Tamachi et al., Biochem Biophys Res Commun 2009 : While no significant difference was observed in the expression of IL-12Rbeta2 and STAT4 between STAT6 ( -/- ) T-bet ( -/- ) CD4 ( + ) T cells and T-bet ( -/- ) CD4 ( + ) T cells, IL-12 induced STAT4 phosphorylation was increased in STAT6 ( -/- ) T-bet ( -/- ) CD4 ( + ) T cells as compared with that in T-bet ( -/- ) CD4 ( + ) T cells
Fujiki et al., International journal of molecular sciences 2008 : Within 24 hours, JTT induced the IL-12 and IL-18 production and later ( 72 hours ) induction of interferon (IFN)-gamma
Mérant et al., Vet Immunol Immunopathol 2009 : This may mean that the lack of IFN-gamma in foals is not due to decreased levels of IL-12 , IL-15 or IL-18, but rather to lower constitutive levels of TNF-alpha
Zhang et al., Acta Pharmacol Sin 2009 : IL-12 suppression, enhanced endocytosis and up-regulation of MHC-II and CD80 in dendritic cells during experimental endotoxin tolerance
Skrzypek et al., Microbes Infect 2009 : Indeed, specific Syk inhibition significantly suppressed the production of IL-12 , IL-6, and TNF-alpha
Chang et al., Blood 2009 (Lymphoma) : IL-12 activates STAT4 , which is a critical regulator of inflammation and T helper type I ( Th1 ) lineage development in murine systems
Dil et al., Mol Immunol 2009 : In contrast, p110 delta signaling was clearly not required for IL-12 production, with p110 delta-mutant B cells in fact showing enhanced IL-12 p70 production
Giraldo et al., Neuroimmunomodulation 2009 (Candidiasis, Vulvovaginal...) : IFN-gamma increased significantly only immediately after the intense exercise ; IL-1beta increased following both exercise intensities, although at 24 h it only remained elevated after the intense exercise ; IL-12 only increased 24 h after the intense exercise, and IL-2 only showed a significant decrease following the moderate exercise
Utsugi et al., J Immunol 2009 (MAP Kinase Signaling System) : Knockdown of PI3K p110alpha by siRNA reduced LPS induced IL-12 protein production in both cell types ... Knockdown of PI3K p110alpha by siRNA reduced LPS induced IL-12 protein production in both cell types
Truong et al., J Leukoc Biol 2009 (Diabetes Mellitus, Experimental) : Anti-BTLA inhibited anti-PD-1 potentiated total IL-12 ( p40+p70 ) production, suggesting the possibility that anti-BTLA may have a greater effect in the setting of anti-PD-1 triggered diabetes
Chaung et al., Vet Immunol Immunopathol 2009 : Zymosan enhanced dectin-1/TLR2/TLR4 expression and TNF-alpha/IL-10 production in all of three types of cell, whereas p-beta-glucan increased dectin-1/TLR4 and TNF-alpha/IL-12 production in AMs but inhibited IL-10 in mDCs ... Zymosan enhanced dectin-1/TLR2/TLR4 expression and TNF-alpha/IL-10 production in all of three types of cell, whereas p-beta-glucan increased dectin-1/TLR4 and TNF-alpha/IL-12 production in AMs but inhibited IL-10 in mDCs ... Zymosan enhanced dectin-1/TLR2/TLR4 expression and TNF-alpha/IL-10 production in all of three types of cell, whereas p-beta-glucan increased dectin-1/TLR4 and TNF-alpha/IL-12 production in AMs but inhibited IL-10 in mDCs ... Zymosan enhanced dectin-1/TLR2/TLR4 expression and TNF-alpha/IL-10 production in all of three types of cell, whereas p-beta-glucan increased dectin-1/TLR4 and TNF-alpha/IL-12 production in AMs but inhibited IL-10 in mDCs ... Zymosan enhanced dectin-1/TLR2/TLR4 expression and TNF-alpha/IL-10 production in all of three types of cell, whereas p-beta-glucan increased dectin-1/TLR4 and TNF-alpha/IL-12 production in AMs but inhibited IL-10 in mDCs
Albright et al., Immunol Lett 2009 (Colitis) : Recombinant IL-10 suppressed and anti-IL-10 receptor antibody increased IFN-gamma, IL-17 and IL-12/IL-23 p40 production in bacterial lysate pulsed APC and plus CD4 ( + ) T cell co-cultures
Yang et al., PloS one 2009 (Leukemia, Lymphocytic, Chronic, B-Cell...) : Moreover, Toll-like receptor ( TLR ) -dependent IL6 and IL12P40 production induced by lipopolysaccharide (LPS) , R837 or CpG ODN 1826 was reduced in IRF-5 ( P68 ) expressing cells as compared to the control cells
Miyazato et al., Infect Immun 2009 (Candidiasis) : C. albicans DNA induced interleukin-12p40 (IL-12p40) production and CD40 expression by murine bone marrow derived myeloid dendritic cells ( BM-DCs ) in a dose dependent manner
Lotter et al., PLoS Pathog 2009 (Disease Models, Animal...) : IFN-gamma production by NKT cells requires the presence of CD1d and simultaneously TLR receptor signalling through MyD88 and secretion of IL-12
Narita et al., Leuk Res 2009 (Leukemia, Plasma Cell) : Production of IFN-alpha and IL-12 in PMDC05 was enhanced by the stimulation with CpG-A and LPS , respectively
Jiang et al., Invest Ophthalmol Vis Sci 2009 (Autoimmune Diseases...) : TLR3 ligation increased the expression of MHC and costimulatory molecules and induced the production of IL-6, IL-12 , and IL-23 by RACs
Hayashi et al., FEMS Immunol Med Microbiol 2009 : The stronger induction of IL-12 and TNF-alpha in the presence of IFN-gamma was also observed by trehalose 6,6'-dimycolate ( TDM ) extracted from BCG-Japan than by TDM from BCG-Connaught, which lacks the methoxymycolate residue
Ablasser et al., J Immunol 2009 : TLR8 was responsible for IL-12 induction upon endosomal delivery of ssRNA oligonucleotides and RIG-I was responsible for IFN-alpha production upon delivery of 5 ' triphosphate RNA into the cytosol ... TLR8 was responsible for IL-12 induction upon endosomal delivery of ssRNA oligonucleotides and RIG-I was responsible for IFN-alpha production upon delivery of 5 ' triphosphate RNA into the cytosol
Reyes et al., Infect Genet Evol 2009 (Genetic Predisposition to Disease...) : Three parameters of macrophage activation were assessed during Taenia infection : LPS+IFN-gamma induced production of IL-12 , TNF-alpha and nitric oxide ( NO ) in vitro ; infection induced markers for alternatively activated macrophages ( Arginase-1, RELM-alpha, Ym-1 and TREM-2 expression ) and suppressive activity ... Three parameters of macrophage activation were assessed during Taenia infection : LPS+IFN-gamma induced production of IL-12 , TNF-alpha and nitric oxide ( NO ) in vitro ; infection induced markers for alternatively activated macrophages ( Arginase-1, RELM-alpha, Ym-1 and TREM-2 expression ) and suppressive activity ... The maximum response to LPS+IFN-gamma induced TNF-alpha, IL-12 and NO production by macrophages from both strains of mice occurred 2 wk post-infection ... The maximum response to LPS+IFN-gamma induced TNF-alpha, IL-12 and NO production by macrophages from both strains of mice occurred 2 wk post-infection
Eguchi et al., Expert Rev Vaccines 2009 : IFN-gamma could be required for optimal IL-12 production
Ortega et al., J Leukoc Biol 2009 (Psoriasis) : These cells are refractory to Tregs but show a proliferative response to anti-CD3/CD28 stimulation that is enhanced by IL-12 and IL-15
la Sala et al., J Exp Med 2009 (Toxoplasmosis) : Therefore, because IRF8 is essential for IL-12 production and the differentiation of CD8alpha ( + ) cDCs and pDCs, these data suggest that CT and other Gs-protein agonists can affect IL-12 production and DC differentiation via a common mechanism involving IRF8
Wang et al., Transpl Int 2009 : RAPA affected maturation, increased apoptosis and reduced lipopolysaccharide (LPS) induced IL-12 and IL-10 production in iDCs
Caruso et al., Gastroenterology 2009 (Colitis, Ulcerative...) : Because Th1 and Th2 cells, and the cytokines they release, are often mutually antagonistic, we examined whether IL-25 affects IL-12 production or Th1 cell mediated inflammation in the gut
Martner et al., Infect Immun 2009 : If pneumococcal LytA was inactivated by mutation or by culture in a medium containing excess choline, the pneumococci induced production of significantly more TNF, IFN-gamma, and IL-12 in PBMC, whereas the production of IL-6, IL-8, and IL-10 was unaffected ... If pneumococcal LytA was inactivated by mutation or by culture in a medium containing excess choline, the pneumococci induced production of significantly more TNF, IFN-gamma, and IL-12 in PBMC, whereas the production of IL-6 , IL-8, and IL-10 was unaffected ... If pneumococcal LytA was inactivated by mutation or by culture in a medium containing excess choline, the pneumococci induced production of significantly more TNF, IFN-gamma, and IL-12 in PBMC, whereas the production of IL-6, IL-8 , and IL-10 was unaffected ... Further, adding autolyzed pneumococci to intact bacteria inhibited production of TNF, IFN-gamma, and IL-12 in a dose dependent manner but did not inhibit production of IL-6, IL-8, and IL-10 in response to the intact bacteria ... Further, adding autolyzed pneumococci to intact bacteria inhibited production of TNF, IFN-gamma, and IL-12 in a dose dependent manner but did not inhibit production of IL-6 , IL-8, and IL-10 in response to the intact bacteria ... Further, adding autolyzed pneumococci to intact bacteria inhibited production of TNF, IFN-gamma, and IL-12 in a dose dependent manner but did not inhibit production of IL-6, IL-8 , and IL-10 in response to the intact bacteria
Wong et al., J Immunol 2009 : IL-12p70 production was produced predominantly by CD8alpha ( + ) DCs and plasmacytoid DCs, and mediated by CD8 T cell derived cytokines IFN-gamma, GM-CSF, TNF-alpha , and surface CD40L ... IL-12p70 production was produced predominantly by CD8alpha ( + ) DCs and plasmacytoid DCs, and mediated by CD8 T cell derived cytokines IFN-gamma, GM-CSF, TNF-alpha, and surface CD40L ... IL-12p70 production was produced predominantly by CD8alpha ( + ) DCs and plasmacytoid DCs, and mediated by CD8 T cell derived cytokines IFN-gamma, GM-CSF , TNF-alpha, and surface CD40L ... IL-12p70 production was produced predominantly by CD8alpha ( + ) DCs and plasmacytoid DCs, and mediated by CD8 T cell derived cytokines IFN-gamma , GM-CSF, TNF-alpha, and surface CD40L
Durães et al., Immunol Lett 2009 : IL-12 and TNF-alpha production by dendritic cells stimulated with Schistosoma mansoni schistosomula tegument is TLR4- and MyD88 dependent
Uemura et al., J Immunol 2009 : This study demonstrates that the activation of human iNKT cells by their specific ligand alpha-galactosylceramide enhances IL-12p70 while inhibiting the IL-23 production by monocyte derived dendritic cells, and in turn down regulating the IL-17 production by memory CD4 ( + ) Th cells
Kim et al., Int Immunol 2009 : gamma-PGA induced the expression of IL-12p40 , CD80 and CD86 in dendritic cells ( DC ) and macrophages in a Toll-like receptor-4 dependent manner, and the effect of gamma-PGA on T ( h ) 1/T ( h ) 2 development was dependent on the presence of antigen presenting cells (APC) ... gamma-PGA induced the expression of IL-12p40 , CD80 and CD86 in dendritic cells ( DC ) and macrophages in a Toll-like receptor-4 dependent manner, and the effect of gamma-PGA on T ( h ) 1/T ( h ) 2 development was dependent on the presence of antigen presenting cells (APC) ... gamma-PGA induced the expression of IL-12p40 , CD80 and CD86 in dendritic cells ( DC ) and macrophages in a Toll-like receptor-4 dependent manner, and the effect of gamma-PGA on T ( h ) 1/T ( h ) 2 development was dependent on the presence of antigen presenting cells (APC)
Correa et al., Glia 2010 (MAP Kinase Signaling System) : Moreover, endogenously produced IL-10 negatively regulates IL-12 and IL-23 cytokines, which in its turn modify the pattern of expression of transcription factors involved in Th commitment of splenocytes
Brereton et al., J Immunol 2009 (Autoimmune Diseases) : In this study, we show that inhibition of ERK MAPK suppressed IL-23 and IL-1beta production by dendritic cells stimulated with TLR or dectin-1 agonists but did not affect IL-12p70 production
Li et al., J Infect Dis 2009 (Chancroid) : More lesional NK cells than peripheral blood NK cells produced IFN-gamma in response to IL-12 and IL-18
Bluth et al., International journal of medical sciences 2009 (Hepatitis, Autoimmune) : In contrast, before treatment, IL-12 and TNFalpha were not detected in serum ; however after treatment the levels of IL-12 and TNFalpha dramatically increased
Brahmachari et al., J Immunol 2009 : IL-12p40 ( 2 ) dose-dependently inhibited the expression of Foxp3 and CD25, but not CD4 ... IL-12p40 ( 2 ) dose-dependently inhibited the expression of Foxp3 and CD25 , but not CD4 ... IL-12p40 ( 2 ) dose-dependently inhibited the expression of Foxp3 and CD25, but not CD4
Yu et al., J Allergy Clin Immunol 2009 (Common Variable Immunodeficiency) : We quantitated TNF-alpha, IL-6, and IL-12 production in response to TLR1-9 ligands and measured IFN-alpha production by TLR7 stimulated PBMCs and isolated plasmacytoid dendritic cells ( pDCs )
Schmitt et al., Immunity 2009 : IL-12 induced two different IL-21-producers : IL-21 ( + ) IFN-gamma ( + ) T-bet ( + ) Th1 cells and IL-21 ( + ) IFN-gamma ( - ) T-bet ( - ) non-Th1 cells, in a manner dependent on signal transducer and activator of transcription 4 ( STAT4 ) ... IL-12 also regulated IL-21 secretion by memory CD4 ( + ) T cells
Nold-Petry et al., J Biol Chem 2009 (MAP Kinase Signaling System) : In addition, we silenced IL-18Ralpha with small interfering RNA in primary human blood cells and observed up to 4-fold increases in the secretion of lipopolysaccharide- and IL-12/IL-18 induced IL-1beta, IL-6, interferon-gamma, and CD40L ... In addition, we silenced IL-18Ralpha with small interfering RNA in primary human blood cells and observed up to 4-fold increases in the secretion of lipopolysaccharide- and IL-12/IL-18 induced IL-1beta, IL-6, interferon-gamma , and CD40L ... In addition, we silenced IL-18Ralpha with small interfering RNA in primary human blood cells and observed up to 4-fold increases in the secretion of lipopolysaccharide- and IL-12/IL-18 induced IL-1beta , IL-6, interferon-gamma, and CD40L ... In addition, we silenced IL-18Ralpha with small interfering RNA in primary human blood cells and observed up to 4-fold increases in the secretion of lipopolysaccharide- and IL-12/IL-18 induced IL-1beta, IL-6 , interferon-gamma, and CD40L
Mansilla et al., J Hepatol 2009 (Disease Models, Animal...) : EDA-NS3 , but not NS3 alone, upregulated the expression of maturation markers, as well as Delta-like 1 and Delta-like 4 Notch ligands in DC and induced the production of IL-12
Reske et al., Neuroimmunomodulation 2009 (Multiple Sclerosis) : GLAT reduced IL12p40 ( p < 0.001 ), IFNgamma ( p = 0.001 in CD4+ and CD8+ lymphocytes ) and TNF production of leukocytes ( p < 0.001 )
Renneson et al., Eur J Immunol 2009 : Neonatal myeloid DC were shown to be deficient in IFN-beta and IL-12 synthesis in response to TLR triggering
Saraiva et al., Immunity 2009 : We showed that development of IL-10 producing Th1 cells required high T cell receptor ( TCR ) ligation, sustained ERK1 and ERK2 MAP kinases phosphorylation, and IL-12 induced STAT4 transcription factor activation ... We showed that development of IL-10 producing Th1 cells required high T cell receptor ( TCR ) ligation, sustained ERK1 and ERK2 MAP kinases phosphorylation, and IL-12 induced STAT4 transcription factor activation ... We showed that development of IL-10 producing Th1 cells required high T cell receptor ( TCR ) ligation, sustained ERK1 and ERK2 MAP kinases phosphorylation, and IL-12 induced STAT4 transcription factor activation
Belderbos et al., Clin Immunol 2009 : Skewed pattern of Toll-like receptor 4-mediated cytokine production in human neonatal blood : low LPS induced IL-12p70 and high IL-10 persist throughout the first month of life ... In contrast, TLR4 demonstrated a slower maturation, with low LPS induced IL-12p70 production and high IL-10 production up until the age of one month
Yoshimatsu et al., Bone 2009 : IL-12 inhibits TNF-alpha induced osteoclastogenesis via a T cell independent mechanism in vivo ... We previously reported that IL-12 induces apoptosis in bone marrow cells treated with TNF-alpha in vitro via an interaction between TNF-alpha induced Fas and IL-12 induced Fas ligand (FasL) , and that, as a result, osteoclastogenesis is inhibited ... These results suggest that IL-12 inhibits TNF-alpha mediated osteoclastogenesis and induces apoptotic changes through an interaction between TNF-alpha induced Fas and IL-12 induced FasL, in vivo, via a T cell independent mechanism ... These results suggest that IL-12 inhibits TNF-alpha mediated osteoclastogenesis and induces apoptotic changes through an interaction between TNF-alpha induced Fas and IL-12 induced FasL , in vivo, via a T cell independent mechanism
Massacand et al., Proc Natl Acad Sci U S A 2009 (Strongylida Infections...) : TSLP inhibited IL-12p40 production in response to T. muris infection, and treatment of TSLPR ( -/- ) animals with neutralizing anti-IL-12p40 monoclonal antibody ( mAb ) was able to reverse susceptibility and attenuate IFN-gamma production ... Taken together, our data show that the primary function of TSLP is to directly suppress IL-12 secretion, thus supporting Th2 immune responses
Ju et al., Eur J Immunol 2009 (Melanoma, Experimental) : Activation of p38 MAP kinase, but not ERK or JNK, by either TCR-stimuli or IL-12 and IL-18 is diminished in Gadd45b ( -/- ) CD8 ( + ) T cells, resulting in reduced production of IFN-gamma
Gómez-Laguna et al., J Comp Pathol 2010 (Porcine Reproductive and Respiratory Syndrome) : The results also suggest that expression of IFN-gamma is stimulated by IL-12p40 and TNF-alpha, but not by IFN-alpha
Toh et al., Cytokine 2009 : IL-17 decreased IL-12 induced IFN-gamma expression in PBMC and developing Th1 cells, associated with a selective reduction in IL-12R beta 2, and not IL-23R, IL-12R beta 1 or T-bet
Haddad et al., J Immunol 2009 : In contrast, B cell depletion almost completely inhibits IL-12 induced IFN-gamma production and B cell-deficient spleen cells are poorly stimulated with IL-12 ... Cell contact between B and NK cells is also required for IL-12 induced IFN-gamma production
Li et al., J Immunol 2009 (Alcoholic Intoxication...) : Because IL-12 plays a major role in Th1 differentiation and IFN-gamma production, we examined whether diminished IL-2 and IFN-gamma production after EtOH plus burn injury resulted from a decrease in IL-12 ... Because IL-12 plays a major role in Th1 differentiation and IFN-gamma production, we examined whether diminished IL-2 and IFN-gamma production after EtOH plus burn injury resulted from a decrease in IL-12
Good et al., J Immunol 2009 : Although many STAT4 target genes display STAT4 dependent IL-12-inducible expression, other genes displayed IL-12 induced histone modifications but lack induction, possibly due to high relative basal expression
Lin et al., J Neurochem 2009 : Context dependent IL-6 potentiation of interferon- gamma induced IL-12 secretion and CD40 expression in murine microglia ... Using highly enriched cultures of neonatal murine microglia we show that IL-6 alone has direct effects on microglia as it activates STAT3 and extracellular signal regulated kinase pathways in a time- and dose dependent fashion and it enhances interferon-gamma (IFNgamma) stimulated IL-12 secretion ... Using highly enriched cultures of neonatal murine microglia we show that IL-6 alone has direct effects on microglia as it activates STAT3 and extracellular signal regulated kinase pathways in a time- and dose dependent fashion and it enhances interferon-gamma (IFNgamma) stimulated IL-12 secretion
Ma et al., Immunol Cell Biol 2009 : However, only IL-12 induced sustained expressions of CXCR5 and ICOS on these activated naïve CD4 ( + ) T cells, and endowed them with the ability to provide increased help to B cells for their differentiation into immunoglobulin secreting cells ... However, only IL-12 induced sustained expressions of CXCR5 and ICOS on these activated naïve CD4 ( + ) T cells, and endowed them with the ability to provide increased help to B cells for their differentiation into immunoglobulin secreting cells
Cho et al., Immunol Lett 2009 : Programmed death-1 receptor negatively regulates LPS mediated IL-12 production and differentiation of murine macrophage RAW264.7 cells ... PD-1 mediated suppression of IL-12 production in LPS stimulated RAW264.7 cells was mediated by inhibition of Janus N-terminal linked kinase ( JNK ) signaling pathway, and to a lesser extent, phosphatidylinositol 3-kinase (PI3K)/protein kinase B ( Akt ) pathway through the recruitment of SHP-2 to PD-1 cytoplasmic tail
Jeong et al., Int Immunopharmacol 2009 (Dental Plaque...) : Apigenin significantly inhibited the nicotine- and LPS induced production of NO, PGE2, IL-1beta, TNF-alpha, IL-6, and IL-12 , and the upregulation of iNOS and COX-2 in hPDL cells
Qiao et al., BMC cell biology 2009 : siRNA PAR-1.2 and siRNA PAR-4.2 significantly reduced IL-12 induced upregulation of PAR-1 and PAR-4 expression, respectively when P815 cells were transfected with them for 48 h. siRNA PAR-2.3 blocked IL-12 induced downregulation of PAR-2 expression on both mRNA and protein levels
Cuong et al., Life Sci 2009 (Inflammation...) : Pre-treatment with C-K significantly inhibited zymosan mediated secretion of tumor necrosis factor-alpha, interleukin (IL)-6, and IL-12 p40, and the activation of ERK1/2 and p38 ... Pre-treatment with C-K significantly inhibited zymosan mediated secretion of tumor necrosis factor-alpha, interleukin (IL)-6, and IL-12 p40, and the activation of ERK1/2 and p38
Garcia et al., J Immunol 2009 : c-jun controls the ability of IL-12 to induce IL-10 production from human memory CD4+ T cells ... In the present study, we demonstrate that IL-12p70 induces IL-10 production from human memory CD4+ T cells via a PI3K dependent signaling mechanism ... In the present study, we demonstrate that IL-12p70 induces IL-10 production from human memory CD4+ T cells via a PI3K dependent signaling mechanism ... In contrast, direct inhibition of GSK3 mimicked the effect of IL-12p70 on IL-10 production by memory CD4+ T cells ... The ability of IL-12p70 to inactivate GSK3beta and induce c-jun levels was required for IL-12 to augment IL-10 production by human memory CD4+ T cells, since small interfering RNA mediated gene silencing of c-jun abrogated this process ... These studies identify the cellular mechanism by which IL-12 induces IL-10 production from human memory CD4+ T cells
Kang et al., Immunology 2009 : Knock-down of IL-32beta by IL-32 small interfering RNA led to the decrease of IL-10, but the increase of IL-12 in monocyte derived DC, which means that IL-32beta promotes IL-10 production, but limits IL-12 production ... We also showed that IL-10 neutralization increases IL-12 , IL-1beta and tumour necrosis factor-alpha production, which implies that IL-10 suppresses such proinflammatory cytokines
Jacques et al., Immunology 2009 (Acute Disease...) : Ex vivo and in vitro experiments revealed that NK cells, expressing carcinoembryonic antigen related cell adhesion molecules ( CEACAM) 1a ( the MHV receptor ), can produce a higher level of IFN-gamma in the presence of both L2-MHV3 and interleukin-12 (IL-12)/IL-18
Rottinghaus et al., Immunology 2009 : We have identified a population of CD44 ( hi ) CD8 T cells in old mice that are capable of secreting interferon-gamma (IFN-gamma) in response to interleukin-12 (IL-12) stimulation ... Furthermore, we demonstrate that IL-12 induced STAT-4 activation was required for T helper type 1 ( Th1 ) cytokine induced IFN-gamma production in CD8 T cells ... Furthermore, we demonstrate that IL-12 induced STAT-4 activation was required for T helper type 1 ( Th1 ) cytokine induced IFN-gamma production in CD8 T cells
Rovedatti et al., Gut 2009 (Colitis, Ulcerative...) : IL23 and IL1beta plus IL6 had no effect on IBD LPMC production of IL17 ; however, IL12 markedly increased IFNgamma production and decreased IL17 production ... IL23 and IL1beta plus IL6 had no effect on IBD LPMC production of IL17 ; however, IL12 markedly increased IFNgamma production and decreased IL17 production ... IL23 and IL1beta plus IL6 had no effect on IBD LPMC production of IL17 ; however, IL12 markedly increased IFNgamma production and decreased IL17 production ... IL23 and IL1beta plus IL6 had no effect on IBD LPMC production of IL17 ; however, IL12 markedly increased IFNgamma production and decreased IL17 production ... Our findings support a role for IL12 , TGFbeta and IL21 in modulating IL17/IFNgamma production in IBD ... Our findings support a role for IL12 , TGFbeta and IL21 in modulating IL17/IFNgamma production in IBD
Balic et al., Immunol Lett 2009 (Intestinal Diseases, Parasitic...) : Further, infection alters TLR responsiveness, as IL-12 production ( as measured by ex vivo intracellular staining of CD11c ( + ) DCs ) in response to LPS stimulation is reduced, while IL-6, TNF-alpha and in particular, IL-10 all increase following infection with either nematode parasite
Jones et al., J Immunol 2009 : Neither the genetic absence of IL-4, IL-4Ralpha chain, STAT-6, IFN-gamma, or IL-12p40 nor mAb blockade of IFN-gamma, IL-3, IL-4, IL-5, IL-6, IL-10, IL-13, IL-17A, IL-12p40, or IL-12p40Rbeta1 before Ag challenge in WT mice reduces the pulmonary MCp increase
Gao et al., Cancer Lett 2010 (Carcinoma, Renal Cell...) : Further studies showed that the anti-tumor activity of the MSC/IL-12 was dependent on the presence of natural killer ( NK ) cells and IFN-gamma in this experimental setting
Kaden et al., J Immunol 2009 : Intriguingly, upon triggering mDCAR1, CD8 ( + ) DCs increased the secretion of bioactive IL-12 , whereas IL-10 release is markedly reduced, thereby indicating that Ag recognized by mDCAR1 induces enhanced proinflammatory responses ... Intriguingly, upon triggering mDCAR1, CD8 ( + ) DCs increased the secretion of bioactive IL-12 , whereas IL-10 release is markedly reduced, thereby indicating that Ag recognized by mDCAR1 induces enhanced proinflammatory responses
Correale et al., J Immunol 2009 (Multiple Sclerosis...) : SEA suppressed the LPS induced DCs production of IL-1beta, IL-6, IL-12 , and TNF-alpha and enhanced TGF-beta as well as IL-10 production ... SEA suppressed the LPS induced DCs production of IL-1beta, IL-6, IL-12 , and TNF-alpha and enhanced TGF-beta as well as IL-10 production
Haas et al., Eur J Immunol 2009 : IL-12 and IL-18 induced IFN-gamma and IL-23 induced IL-17A production by NK1.1+ or CCR6+ gammadelta T cells, respectively ... IL-12 and IL-18 induced IFN-gamma and IL-23 induced IL-17A production by NK1.1+ or CCR6+ gammadelta T cells, respectively
Pilling et al., PloS one 2009 : In addition, IL-4, IL-12 , IL-13, IFN-gamma, and SAP differentially regulate the expression of CD32, CD163 , CD172a, and CD206 on both macrophages and fibrocytes ... In addition, IL-4, IL-12 , IL-13, IFN-gamma, and SAP differentially regulate the expression of CD32 , CD163, CD172a, and CD206 on both macrophages and fibrocytes ... In addition, IL-4, IL-12 , IL-13, IFN-gamma, and SAP differentially regulate the expression of CD32, CD163, CD172a, and CD206 on both macrophages and fibrocytes ... In addition, IL-4, IL-12 , IL-13, IFN-gamma, and SAP differentially regulate the expression of CD32, CD163, CD172a , and CD206 on both macrophages and fibrocytes
Gurung et al., J Immunol 2009 : Dendritic cells fed activated apoptotic T cells in vitro produced IL-12p40 in a CD154 dependent manner, and the use of IL-12p40 ( -/- ) mice or mAb mediated neutralization of IL-12 revealed a link between CD154, IL-12, and the ability of activated apoptotic T cells to induce immunity rather than tolerance
Yang et al., Immunol Cell Biol 2010 : The A2b receptor antagonist MRS1754 was able to increase the production of IL-12p70 and TNF-alpha by hypoxic mDCs and elevate the amount of Th1 cytokine IFN-gamma production in a mDCs-T-cell co-culture system
Micera et al., Molecular vision 2009 (Conjunctivitis, Allergic) : These cells exhibited poor IL-4, IL-12 p40, and IFN-gamma responses to NGF, while a significant IL-10 decreased secretion was detected ... These cells exhibited poor IL-4, IL-12 p40, and IFN-gamma responses to NGF , while a significant IL-10 decreased secretion was detected
Lin et al., Immunity 2009 (Tularemia) : Furthermore, we show that IL-17A also induced IL-12 and interferon-gamma production in macrophages and mediated bacterial killing
Ceballos et al., J Exp Med 2009 : Interaction of spermatozoa with DCs not only leads to the transmission of HIV-1 and the internalization of the spermatozoa but also results in the phenotypic maturation of DCs and the production of IL-10 but not IL-12p70
Tuettenberg et al., Exp Dermatol 2010 : Additionally, Ad-CD40L induced strong production of IL-12p70 not observed in cytokine matured DC
Manicone et al., Am J Pathol 2009 (Lung Injury...) : Soluble E-cadherin increased IL-12 and IL-10 and reduced IL-6 mRNA expression in wild-type bone marrow derived DC but not in CD103 ( -/- ) bone marrow derived DC
Yamauchi et al., International journal of biological sciences 2009 : AZM reduced the induction of IL-12p40 by LPS/IFN-gamma in a dose dependent manner ... AZM reduced the induction of IL-12p40 by LPS/IFN-gamma in a dose dependent manner ... AZM also reduced LPS/IFN-gamma induced IL-12p40 promoter activity ... AZM also reduced LPS/IFN-gamma induced IL-12p40 promoter activity
Dar et al., Res Vet Sci 2010 : A-class CpG ODN induced significant but transient levels of IFN-gamma, IL-12 ( P40 ), IL-6, IL-4 and TNF-alpha mRNA, C-class CpG ODN induced significant level of IFN-gamma , IFN-alpha and IL-12 mRNA and the lowest level of IL-4 ( Th-2 type ) mRNA
Zhou et al., Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2009 : When PBMC was stimulated with BCG, the frequency of granzyme B producing cells was higher than that in unstimulated PBMC ( P < 0.05 ). BCG enhanced the cytotoxic activity of PBMC. BCG alone did n't induce IFN-gamma production by purified NK cells, but it can augment IL-12 induced IFN-gamma production by purified NK cells. The cytotoxic activities of BCG stimulated and unstimulated purified NK cells were not significantly different ( P > 0.05 )
Wenink et al., J Immunol 2009 : The inhibitory effect of TLR2 on the release of TNF-alpha but not of IL-12p70 was mediated by PI3K ... The inhibitory effect of TLR2 on the release of TNF-alpha but not of IL-12p70 was mediated by PI3K ... TLR2 inhibits the production of IL-12p70 by dampening the type 1 IFN amplification loop
Kollmann et al., J Immunol 2009 : In response to most TLR ligands, neonatal innate immune cells, including monocytes and conventional and plasmacytoid dendritic cells produced less IL-12p70 and IFN-alpha ( and consequently induced less IFN-gamma ), moderately less TNF-alpha, but as much or even more IL-1beta, IL-6, IL-23, and IL-10 than adult cells
Oyamada et al., J Immunol 2009 (Encephalomyelitis, Autoimmune, Experimental) : Tyrosine kinase 2 (Tyk2) , a member of the JAK family, is involved in IL-12- and IL-23 mediated signaling
Yue et al., J Immunol 2010 (Cardiovirus Infections) : Optimal resistance to infection with picornavirus encephalomyocarditis virus is known to require CD1d dependent APC IL-12 induced IFN-gamma as well as IFN-alpha ... CD1d ligation in vivo enhanced systemic IL-12 , IFN-gamma, and IFN-alpha and was protective against infection by encephalomyocarditis virus, suggesting an alternative interpretation for previous results involving CD1d `` blocking '' in other systems
Langlet et al., Eur J Immunol 2010 : Unexpectedly, BM-derived DC from PKC-alpha ( -/- ) mice exhibited decreased TNF-alpha and IL-12p40 production induced by both MyD88- and TRIF dependent ligands
Reeves et al., AIDS Res Hum Retroviruses 2009 (Simian Acquired Immunodeficiency Syndrome) : FLT3-L also increased serum levels of IL-12 , at least transiently, and elicited higher surface expression of HLA-DR and the activation markers CD25 and CD69 on NK and T cells
Perona-Wright et al., Cell Host Microbe 2009 (Infection) : The proinflammatory cytokine IL-12 was necessary and sufficient for NK cell induction of IL-10 ... NK cells from mice with systemic parasitic infection inhibited dendritic cell release of IL-12 in an IL-10 dependent manner, and NK cell depletion resulted in elevated serum IL-12
Crotta et al., J Hepatol 2010 : We found that immobilized HCVcc, unlike soluble HCVcc, inhibited IFN-gamma production by interleukin (IL)-12 activated NK cells, and that this effect was mediated by engagement of cellular CD81 by HCV-virion displayed E2
van Hoffen et al., Int Arch Allergy Immunol 2010 : Apically added LGG, TLR2 and NOD2 ligands, but not Bb, enhanced IFN-gamma, IL-12 and/or TNF-alpha secretion ... Apically added LGG, TLR2 and NOD2 ligands, but not Bb, enhanced IFN-gamma, IL-12 and/or TNF-alpha secretion
van de Wetering et al., PloS one 2009 (Salmonella Infections) : Both IFN-gamma and GM-CSF enhanced IL-23 production by monocytes in response to TLR agonists, as well as induced IL-12 production ... The IL-23 induced cytokines allow for the subsequent production of IL-12 and amplify the IFN-gamma production in the type-1 cytokine pathway
Goodyear et al., J Immunol 2010 (Burkholderia Infections...) : Production of IL-12 and IFN-gamma in the lungs after B. mallei infection was significantly impaired in both MCP-1 ( -/- ) and CCR2 ( -/- ) mice, whereas treatment of CCR2 ( -/- ) mice with rIFN-gamma restored protection against lethal challenge with B. mallei ... Production of IL-12 and IFN-gamma in the lungs after B. mallei infection was significantly impaired in both MCP-1 ( -/- ) and CCR2 ( -/- ) mice, whereas treatment of CCR2 ( -/- ) mice with rIFN-gamma restored protection against lethal challenge with B. mallei
Ni et al., Br J Pharmacol 2009 (Encephalomyelitis, Autoimmune, Experimental) : In addition, TAK-779 did not affect production of IL-12 by antigen presenting cells, CCR5 induction on T cells and the potential of MOG-specific T cells to transfer EAE
Barbé et al., Vet J 2011 (Orthomyxoviridae Infections...) : The findings suggest that IFN-? and IL-12 play a role in the pathogenesis of SIV and that APPs are induced by cytokines
Xuan et al., Cell Physiol Biochem 2010 : LPS increased the percentage of CD11c ( + ) CD86 ( + ), CD11c ( + ) MHCII ( + ), CD11c ( + ) CD40 ( + ) and CD11c ( + ) CD54 ( + ) cells and stimulated the release of IL-10, IL-12p70 and TNF-alpha
Lech et al., J Pathol 2010 (Genetic Predisposition to Disease...) : Sigirr-deficient dendritic cells expressed higher TLR7 mRNA levels and TLR7 activation resulted in increased IL-12 production in vitro ... In vivo, lack of SIGIRR increased surface CD40 expression on spleen CD11c ( + ) dendritic cells and MX-1, TNF, IL-12 , BAFF and BCL-2 mRNA expression 6 months after pristane injection
Boucher et al., Viral Immunol 2010 (HIV Infections...) : Disparate regulation of LPS induced MAPK signaling and IL-12p40 expression between different myeloid cell types with and without HIV infection ... In primary monocytes, the inhibition of p38 enhanced IL-12 production, whereas it downregulated IL-12 production in THP-1 cells ... The role of MAPK signaling in transcription factor binding to the IL-12p40 promoter was subsequently determined ... In THP-1 cells, p38 , ERK, and JNK inhibition increased NF-kappaB and Sp1 binding to the IL-12p40 promoter, while inhibiting AP-1 binding ... In THP-1 cells, p38, ERK , and JNK inhibition increased NF-kappaB and Sp1 binding to the IL-12p40 promoter, while inhibiting AP-1 binding ... In monocytes, mutations in the NF-kappaB, AP-1, Sp1, or Ets-2 binding sites resulted in complete inhibition of LPS stimulated IL-12p40 promoter activity using a luciferase based assay ... These data clearly show that although LPS induces IL-12p40 expression in primary monocytes and THP-1 cells, the signaling pathways involved and the effect of HIV infection differ and can have disparate effects in these two cell types
Lassen et al., J Immunol 2010 : Compared with splenic NK cells, liver NK cells displayed a dampened IFN-gamma response to IL-12/IL-18 stimulation
Masuda et al., Biosci Biotechnol Biochem 2010 (Diarrhea...) : Next we demonstrated, by neutralizing IL-12 and IFN-gamma, that the Sn26 strain first induced IL-12, that IL-12 induced IFN-gamma , and that decreases in IL-4 and IgE production followed
Zhou et al., J Leukoc Biol 2010 : Protein tyrosine phosphatase SHP-1 positively regulates TLR induced IL-12p40 production in macrophages through inhibition of phosphatidylinositol 3-kinase ... Protein tyrosine phosphatase SHP-1 positively regulates TLR induced IL-12p40 production in macrophages through inhibition of phosphatidylinositol 3-kinase ... Bone marrow derived macrophages ( BMDMs ) lacking significant SHP-1 activity display a profound defect in IL-12p40 synthesis in response to lipopolysaccharide, peptidoglycan, and synthetic TLR ligands, while producing normal amounts of other proinflammatory cytokines, such as TNFalpha and IL-6
Maggini et al., PloS one 2010 : Using mouse thioglycolate elicited peritoneal macrophages ( M ) stimulated with LPS, we found that MSC markedly suppressed the production of the inflammatory cytokines TNF-alpha, IL-6, IL-12p70 and interferon-gamma while increased the production of IL-10 and IL-12p40
Sarra et al., Inflamm Bowel Dis 2010 (Inflammatory Bowel Diseases) : To assess the involvement of IL-12 and IL-23 in the production of IL-21, T-LPL were activated in the presence or absence of IL-12 or IL-23 ... To assess the involvement of IL-12 and IL-23 in the production of IL-21 , T-LPL were activated in the presence or absence of IL-12 or IL-23
Yin et al., Vaccine 2010 (Simian Acquired Immunodeficiency Syndrome) : We further demonstrated that interleukin (IL)-12 could significantly enhance the vaccine specific CD8 lymphocyte response
Kaji et al., J Immunol 2010 : In mouse peritoneal macrophages, Lactobacillus plantarum potently induced IL-10 but weakly induced IL-12 production, whereas L. casei potently induced IL-12 but weakly induced IL-10 production
Lapara et al., Journal of inflammation (London, England) 2010 : Unlike many microbes, the kinetoplastid protozoan pathogen Leishmania has been shown to avoid and even actively suppress host inflammatory cytokine responses, such as LPS induced IL-12 production
Haeberlein et al., Eur J Immunol 2010 (Leishmaniasis, Visceral) : Here, we investigated whether IL-15 or IL-18 mediate the activity of IL-12 or function as independent activators of NK cells ... Here, we investigated whether IL-15 or IL-18 mediate the activity of IL-12 or function as independent activators of NK cells
Horvat et al., J Immunol 2010 (Asthma...) : This phenotype was also characterized by increased pulmonary IL-12 and IL-17 expression and activation of APCs , as well as by reduced thymus- and activation regulated chemokine
Ye et al., PloS one 2010 (Hepatitis C...) : Upregulation of SOCS-3 and PIAS-3 impairs IL-12 mediated interferon-gamma response in CD56 T cells in HCV infected heroin users
Dunne et al., Cancer Immunol Immunother 2010 : Vgamma9Vdelta2 T cells also strongly costimulated IL-12 release but inhibited IL-10 production by lipopolysaccharide (LPS) stimulated DC ... When substituted for Vgamma9Vdelta2 T cells, IFN-gamma did not induce full DC maturation but it augmented IL-12 and inhibited IL-10 release by LPS stimulated DC, in a manner similar to HMB-PP activated Vgamma9Vdelta2 T cells ... When substituted for Vgamma9Vdelta2 T cells, IFN-gamma did not induce full DC maturation but it augmented IL-12 and inhibited IL-10 release by LPS stimulated DC, in a manner similar to HMB-PP activated Vgamma9Vdelta2 T cells
Gouda et al., Egypt J Immunol 2007 (Arthritis, Rheumatoid) : Only IL-12 , but not IL-18, stimulates IFN-gamma production by RAST cells [ mean +/- SD = 246 +/- 15 pg/ml ]
Wilson et al., PLoS Pathog 2010 (Toxoplasmosis, Animal) : Tgd057-specific primary effector cells required IL-12 for the differentiation of KLRG1+ effector subpopulations and IFN-gamma production in response to restimulation with parasite infected cells, but not to restimulation with cognate peptide
Turnquist et al., Blood 2010 (Inflammation) : High IL-12p40/p70 production by CD86 ( lo ) DC resulted from failed down-regulation of glycogen synthase kinase-3 ( GSK-3 ) activity and could not be ascribed to enhanced Akt function ... Thus, GSK-3 activity in DC is regulated via signaling linked to mTOR and modulates their capacity both to produce IL-12p40/p70 and induce forkhead box p3 in CD4 ( + ) T cells under inflammatory conditions
Krummen et al., J Leukoc Biol 2010 : Release of IL-12 by dendritic cells activated by TLR ligation is dependent on MyD88 signaling, whereas TRIF signaling is indispensable for TLR synergy
Bhan et al., PloS one 2010 (Pneumonia, Bacterial) : Early lung expression of TNF-alpha, IL-12, and chemokines was TLR4 dependent, while IFN-gamma production and the later expression of TNF-alpha and IL-12 was dependent on TLR9 ... Early lung expression of TNF-alpha, IL-12 , and chemokines was TLR4 dependent , while IFN-gamma production and the later expression of TNF-alpha and IL-12 was dependent on TLR9
Tomizawa et al., Rheumatology (Oxford) 2010 (Glomerulonephritis...) : In contrast, the IL-1alpha level increased, and IL-17 and IL-12p70 slightly increased with DSG treatment
Ramgolam et al., Endocr Metab Immune Disord Drug Targets 2010 (Multiple Sclerosis, Relapsing-Remitting) : IFNbeta induces the production of the Th17-suppressive cytokines interleukin (IL)-27 and IL-12 in DCs and B-cells through the phosphorylation of signal transducers and activators of the transcription protein ( STAT ) 1
Marin-Esteban et al., J Immunother 2010 (Neoplasms) : LPS stimulation of DCs induced IL-10, IL-12p70 , TNFalpha and IL-1beta secretion, and taxol pretreatment modified this response by down regulating IL-1beta secretion whereas colchicine induced a proinflammatory cytokine profile with reduced IL-10 and increased IL-12p70 and TNFalpha secretion ... LPS stimulation of DCs induced IL-10, IL-12p70 , TNFalpha and IL-1beta secretion, and taxol pretreatment modified this response by down regulating IL-1beta secretion whereas colchicine induced a proinflammatory cytokine profile with reduced IL-10 and increased IL-12p70 and TNFalpha secretion
Hartiala et al., J Immunol 2010 : The data show that 1 ) effective signaling via p38 MAPK ( and STAT1 and NF-kappaB ) is needed for CD38 expression and 2 ) TLR2 stimulation, as opposed to TLR4 stimulation, does not induce IFN-beta autocrine loop dependent expression of CD38 and secretion of IL-12
Chang et al., Proc Natl Acad Sci U S A 2010 : In this study, we showed that the production of IL-23 but not IL-12 was negatively regulated by protein phosphatase 2A (PP2A) in dendritic cells ( DC )
Hayashi et al., Immunology 2010 : In the present study, we examined the effect of prostaglandin E ( 2 ) ( PGE ( 2 ) ) treatment of DCs during differentiation on IL-12 and IL-23 production in response to Toll-like receptor ( TLR ) stimulation
Charoensit et al., Cancer Gene Ther 2010 (Disease Models, Animal...) : ATRA-cationic liposome/IL-12 pDNA complexes increased the TNFR1 mRNA upregulation and the number of apoptotic cells in the lung
Kubota et al., Scand J Immunol 2010 (Staphylococcal Infections) : However, a number of reports have shown that bacterial stimuli are unable to induce macrophages and/or DCs to produce sufficient amounts of IL-12p70 unless these cells are primed by IFN-gamma
Geluk et al., Clinical and vaccine immunology : CVI 2010 (Leprosy) : We found that only interleukin 12 (IL-12) , not other costimulants, increased IFN-gamma production in WBA while maintaining M. leprae peptide specificity, as evidenced by lack of increase of IFN-gamma in control samples stimulated with IL-12 alone ... The IL-12 induced increase in IFN-gamma was mainly mediated by CD4+ T cells that did not produce IL-2 or tumor necrosis factor (TNF)
Johansson et al., Immunobiology 2011 : CD40 ligation by a CD40L transfected cell-line induced IL-12p70 in DC regardless of previous apoptotic cell uptake
Nguyen et al., PloS one 2010 : In response to LPS , production of TNF-alpha, IP-10 and IL-12p70 reached adult levels between 6-9 months of life
Logue et al., Antiviral Res 2010 (Disease Models, Animal...) : Mice treated with CLDC had significantly increased serum IFN-gamma, TNF-alpha, and IL-12 , suggesting a strong Th1 biased antiviral activation of the innate immune system
Hamza et al., International journal of molecular sciences 2010 : IL-12 induces interferon-gamma (IFN-gamma) production and triggers CD4 ( + ) T cells to differentiate into type 1 T helper ( Th1 ) cells ... IL-12 induces interferon-gamma (IFN-gamma) production and triggers CD4 ( + ) T cells to differentiate into type 1 T helper ( Th1 ) cells
Negishi et al., Am J Reprod Immunol 2011 : ( ii ) TNF-a was also induced by LPS but was not affected by the presence of IL-2 and IL-12 ... ( ii ) TNF-a was also induced by LPS but was not affected by the presence of IL-2 and IL-12
Aerts et al., Clin Exp Rheumatol 2010 (Arthritis, Rheumatoid) : Peripheral blood mononuclear cells ( PBMC ) were analysed flow cytometrically either directly after isolation or after 24 hours of anti-CD3/anti-CD28 stimulation, to determine spontaneous and IL-4/IL-12 induced STAT4 and STAT6 phosphorylation in CD4+ T cells ... Peripheral blood mononuclear cells ( PBMC ) were analysed flow cytometrically either directly after isolation or after 24 hours of anti-CD3/anti-CD28 stimulation, to determine spontaneous and IL-4/IL-12 induced STAT4 and STAT6 phosphorylation in CD4+ T cells
Utsugi et al., Cytokine 2010 : This study defined the distinct regulation of lipopolysaccharide (LPS) mediated IL-12 production by c-jun NH ( 2 ) -terminal kinase ( JNK ) 1 and JNK2 isoforms in human macrophages ... Knockdown of JNK1 and JNK2 by small interference RNA ( siRNA ) reduced and enhanced LPS induced IL-12 p40 production in THP-1 macrophage cells, respectively ... The simultaneous knockdown of JNK1 and JNK2 augmented LPS induced IL-12 production as well as a specific JNK inhibitor ... In addition, transfection of siRNA against phosphoinositide 3-kinase (PI3K) p110beta attenuated LPS induced IL-12 production and JNK1 phosphorylation, while not affecting JNK2 phosphorylation ... These findings indicate that JNK1- and JNK2 mediated signaling plays a positive and a negative role, respectively, in LPS induced IL-12 production and PI3K p110beta controls LPS induced JNK1 activation, not JNK2 activation, resulting in the positive regulation of IL-12 production in THP-1 macrophage cells
Xuan et al., Cell Physiol Biochem 2010 : Both LPS and GA stimulated the production of IL-6, IL-10, IL12p70 and TNFalpha in a p38- and/or ERK dependent manner ... Both LPS and GA stimulated the production of IL-6, IL-10, IL12p70 and TNFalpha in a p38- and/or ERK dependent manner
Mitchell et al., J Infect Dis 2010 : IL-12p40 was significantly inhibited by both p38 mitogen activated protein kinase ( MAPK ) and extracellular signal regulated kinase inhibitors, whereas IL-12p70 was inhibited by p38 MAPK inhibitor alone ... IL-12p40 was significantly inhibited by both p38 mitogen activated protein kinase ( MAPK ) and extracellular signal regulated kinase inhibitors, whereas IL-12p70 was inhibited by p38 MAPK inhibitor alone ... IL-12p40 was significantly inhibited by both p38 mitogen activated protein kinase ( MAPK ) and extracellular signal regulated kinase inhibitors, whereas IL-12p70 was inhibited by p38 MAPK inhibitor alone
Antonios et al., J Immunol 2010 (MAP Kinase Signaling System) : Our results showed that p38 MAPK , NF-kappaB, and IRF-1 were involved in IL-12p40 production induced by NiSO ( 4 ) ... Our results showed that p38 MAPK, NF-kappaB, and IRF-1 were involved in IL-12p40 production induced by NiSO ( 4 ) ... Our results showed that p38 MAPK, NF-kappaB , and IRF-1 were involved in IL-12p40 production induced by NiSO ( 4 )
Hussein et al., J Infect Dis 2010 (Helicobacter Infections) : To explain this paradox, we found that dupA1 ( but not dupA2 or the cag PaI ) substantially increased H. pylori induced IL-12p40 and IL-12p70 production from CD14 ( + ) mononuclear cells
Toh et al., Ann Rheum Dis 2010 (Arthritis, Rheumatoid) : RA PBMCs were less responsive to IL-12 induced IFNgamma than HC PBMCs. IL-12 hyporesponsiveness was increased by IL-17 treatment associated with a selective reduction in IL-12Rbeta2, but not IL-23R, IL-12Rbeta1 or T-bet, which was reversed with IL-17R inhibition
Zhang et al., Clin Neurol Neurosurg 2010 (Autoimmune Diseases...) : IFNbeta inhibited IL-1beta, IL-23 and transforming growth factor ( TGF ) -beta ( which induce Th17 cell differentiation ), and induced IL-27, IL-12p35 and IL-10 ( which suppress it ) in dendritic cells (DCs) and B-cells ... IFNbeta inhibited IL-1beta, IL-23 and transforming growth factor ( TGF ) -beta ( which induce Th17 cell differentiation ), and induced IL-27, IL-12p35 and IL-10 ( which suppress it ) in dendritic cells (DCs) and B-cells ... IFNbeta inhibited IL-1beta, IL-23 and transforming growth factor ( TGF ) -beta ( which induce Th17 cell differentiation ), and induced IL-27, IL-12p35 and IL-10 ( which suppress it ) in dendritic cells (DCs) and B-cells ... IFNbeta inhibited IL-1beta , IL-23 and transforming growth factor ( TGF ) -beta ( which induce Th17 cell differentiation ), and induced IL-27, IL-12p35 and IL-10 ( which suppress it ) in dendritic cells (DCs) and B-cells
Kato et al., Glycoconj J 2010 : SIGNR1 ligation on murine peritoneal macrophages induces IL-12 production through NFkappaB activation ... Engagement of SIGNR1 on PEMs with an anti-SIGNR1-specific rat IgM antibody, ERTR9, induced production of IL-12 and tumor necrosis factor (TNF)-alpha from PEMs, while secretion of IL-6 and IL-1beta was not detected with the same treatment ... Engagement of SIGNR1 on PEMs with an anti-SIGNR1-specific rat IgM antibody, ERTR9, induced production of IL-12 and tumor necrosis factor (TNF)-alpha from PEMs, while secretion of IL-6 and IL-1beta was not detected with the same treatment ... These results indicate that SIGNR1 mediated intercellular signaling may induce production of cytokines such as IL-12 through NFkappaB activation
Marshall et al., J Immunol 2010 (Arenaviridae Infections) : IL-12 , IL-15, IL-18, and IFN-gamma were not individually required for sensitization to produce IFN-gamma, but IL-15 was required for upregulation of granzyme B ... IL-12 , IL-15, IL-18, and IFN-gamma were not individually required for sensitization to produce IFN-gamma, but IL-15 was required for upregulation of granzyme B
Nowak et al., PloS one 2010 (Prostatic Neoplasms) : However, despite inducing up-regulation of these activation markers and, hence, delivering positive signals, prostate tumor cells inhibited the IL-12 induced STAT4 phosphorylation in a cell-cell contact dependent but CD1d independent manner
Jung et al., Eur J Pharmacol 2010 : These results suggest that Ursolic acid modulates human dendritic cells function in a fashion that favors Th1 polarization via the activation of IL-12p70 dependent on TLR2 and/or TLR4, and may be used on dendritic cells based vaccines for cancer immunotherapy ... These results suggest that Ursolic acid modulates human dendritic cells function in a fashion that favors Th1 polarization via the activation of IL-12p70 dependent on TLR2 and/or TLR4 , and may be used on dendritic cells based vaccines for cancer immunotherapy
Jeong et al., Eur J Pharmacol 2010 (Inflammation) : Sappanchalcone is seen to inhibit LPS stimulated nitric oxide ( NO ), prostaglandin E ( 2 ) ( PGE ( 2 ) ), interlukine-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha), interlukine-6 (IL-6) and interlukine-12 (IL-12) release in addition to inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression in HPDL cells
Bihl et al., J Immunol 2010 (Leishmaniasis, Cutaneous) : We show that early IFN-gamma secretion by NK cells is controlled by IL-2 and IL-12 and is dependent on CD40/CD40L interaction ... We show that early IFN-gamma secretion by NK cells is controlled by IL-2 and IL-12 and is dependent on CD40/CD40L interaction ... We show that early IFN-gamma secretion by NK cells is controlled by IL-2 and IL-12 and is dependent on CD40/CD40L interaction
Ko et al., Immune network 2010 : We first demonstrate its effects on LPS stimulated pro-inflammatory cytokine production including interleukin (IL)-6 and IL-12p40 ... LPS induced IL-6 and IL-12p40 production in macrophages was suppressed when macrophages were treated with the GSKi peptide
Wu et al., Cytokine 2010 (Diabetes Mellitus, Type 2...) : In DM patients, insulin could suppress IL-12 production from stimulated PBMCs but not with additional glucose treatment
Perrot et al., J Immunol 2010 : Poly ( I:C ) but not polyadenylic-polyuridylic acid synergized with mDC derived IL-12 for IFN-gamma production by acting directly on NK cells
Lemoine et al., Blood 2010 : Interestingly, we demonstrated that MPA inhibited the LPS induced synthesis of tumor necrosis factor-a, IL-12 , and interferon-? ( IFN-? ) in DCs
Liu et al., Nan Fang Yi Ke Da Xue Xue Bao 2010 (Thyroiditis, Autoimmune) : Compared with the positive control groups, the autoantibody and IFN-gamma and IL-12 levels decreased significantly in the treatment group, but the levels of IL-4 and IL-10 were markedly increased ( P < 0.05 ) ... Compared with the positive control groups, the autoantibody and IFN-gamma and IL-12 levels decreased significantly in the treatment group, but the levels of IL-4 and IL-10 were markedly increased ( P < 0.05 )
Yoon et al., Int J Mol Med 2010 (Lung Neoplasms) : While the level of interleukin (IL)-10 secretion from DC-Ad-CC-10 was decreased, the level of IL-12 secretion was increased by CC-10
Gallo et al., Immunol Lett 2010 : In the present work we sought to determine the density of IgG on immune complexes necessary to mediate phagocytosis, inhibit IL-12 production and induce IL-10 production from stimulated macrophages
Liu et al., J Immunol 2010 (Carcinoma, Hepatocellular...) : However, the potential of Gr-1 ( + ) CD11b ( + ) F4/80 ( + ) cells to express IL-12 in response to IFN-gamma could be attenuated by tumor, partially due to the existence of active STAT3 in Gr-1 ( + ) CD11b ( + ) F4/80 ( + ) cells and NTC-Ms from tumor
Qiu et al., Innate Immun 2011 (Carcinoma, Squamous Cell) : Stimulation of NLRP3 with E. coli RNA led to the highest induction of TNF-a, IL-6, IL-12p40 , RANTES and IFN-ß, whereas TLR7, TLR3, TLR9, NOD1 and NOD2 agonists had lower effects
Chen et al., Biomaterials 2010 : The result that MyD88 inhibitor remarkably reduced the IL-12 expression induced by cationic polymers suggested that this stimulation was mainly mediated by toll-like receptor ( TLR ) pathway
Conzelmann et al., Biochem Pharmacol 2010 : We demonstrate that IFN-? induced Janus kinase 1 (JAK1) enhances CD40 induced IL-12p70 release whilst simultaneously inhibiting IL-10 synthesis, resulting in a pro-inflammatory phenotype of CD40L activated dendritic cells (DCs) ... We demonstrate that IFN-? induced Janus kinase 1 (JAK1) enhances CD40 induced IL-12p70 release whilst simultaneously inhibiting IL-10 synthesis, resulting in a pro-inflammatory phenotype of CD40L activated dendritic cells (DCs) ... JAK2 mediated enhancing effects on IL-12p70 but did not inhibit IL-10 release, whereas Tyk2 mediated inhibitory effects on IL-12p70 release in this system ... JAK2 mediated enhancing effects on IL-12p70 but did not inhibit IL-10 release, whereas Tyk2 mediated inhibitory effects on IL-12p70 release in this system ... JAK2 mediated enhancing effects on IL-12p70 but did not inhibit IL-10 release, whereas Tyk2 mediated inhibitory effects on IL-12p70 release in this system ... The mechanism by which complementary IFN-?/JAK activities affect IL-12p70 production involves STAT1 activation and de novo induction of interferon-responsive factors (IRF)-1 and IRF-8
Tu et al., Zhonghua Shao Shang Za Zhi 2010 (Wounds and Injuries) : Compared with those from sirolimus devoid control ( trauma ) groups, IL-12p40 [ ( 560 +/- 34 ), ( 540 +/- 29 ) pg/mL ], IL-12p70 [ ( 55 +/- 8 ), ( 60 +/- 11 ) pg/mL ] secretion by DC from sirolimus treated control ( trauma ) groups were obviously enhanced ( with F value respectively 218.646, 310.253, P values all below 0.01 ), while IL-10 secretion levels were significantly decreased ( F = 246.108, P < 0.01 )
Dong et al., Phytomedicine 2011 : Furthermore, specific pharmacological inhibitors of ERK kinase ( U0126 ) and p38 MAPK ( SB203580 ) also suppressed the production of IL-12 induced by garlic lectin
Clarke et al., MAbs 2010 : Antibodies with this unique mechanism of inhibition are potent inhibitors of IL-12 induced IFN-? production and IL-23 induced IL-17 production in vitro, and in an in vivo model of psoriasis, treatment with a humanized variant of this antibody, h6F6, reduced the inflammatory response, resulting in decreased epidermal hyperplasia
Feng et al., J Parasitol 2010 : All tachyzoite and antigen preparations at high doses stimulated high levels of interleukin (IL) -12 , interferon (IFN) -gamma, and tumor necrosis factor (TNF) -alpha, except for heat killed tachyzoites and sNcAg, which induced moderate level of IL-12 and very low levels of IFN-gamma and TNF-alpha ... All tachyzoite and antigen preparations at high doses stimulated high levels of interleukin (IL) -12 , interferon (IFN) -gamma, and tumor necrosis factor (TNF) -alpha, except for heat killed tachyzoites and sNcAg, which induced moderate level of IL-12 and very low levels of IFN-gamma and TNF-alpha
Outtz et al., J Immunol 2010 (Inflammation) : Inhibition of Notch signaling decreased induction of the inflammatory cytokines IL-6, IL-12 , CXCL10, MCP-1, monokine induced by IFN-?, and TNF-a in macrophages in response to LPS/IFN-?
Zhang et al., Cell Physiol Biochem 2010 : The results showed that TNF induced up to 2.7-fold increase in IL-4, but not IL-12 release from P815 cells, and PAR-2 antagonist peptide FSLLRY-NH ( 2 ) and PAR-4 antagonist peptide trans-cinnamoyl ( tc ) -YPGKF-NH ( 2 ) did not affect TNF induced IL-4 release
Yang et al., Cell Physiol Biochem 2010 : Since IL-12 plays important roles in protection of the body from microorganism infection and mast cell is a crucial source of IL-12, we investigated effects of IL-12 on expression of TLR2 and TLR4, and cytokine production in mast cells by using quantitative real time PCR, flow cytometry analysis and cellular activation of signaling ELISA techniques ... Since IL-12 plays important roles in protection of the body from microorganism infection and mast cell is a crucial source of IL-12, we investigated effects of IL-12 on expression of TLR2 and TLR4 , and cytokine production in mast cells by using quantitative real time PCR, flow cytometry analysis and cellular activation of signaling ELISA techniques ... IL-12 not only by itself, but also synergistically enhanced LPS induced IL-13 release from P815 cells ... It appears that IL-12 induced IL-13 release and TLR4 expression is through activation of MAPK and PI3K/Akt signaling pathways, whereas IL-12 induced upregulation of TLR2 is via activation of PI3K/Akt signaling pathway, but not MAPK pathway ... It appears that IL-12 induced IL-13 release and TLR4 expression is through activation of MAPK and PI3K/Akt signaling pathways, whereas IL-12 induced upregulation of TLR2 is via activation of PI3K/Akt signaling pathway, but not MAPK pathway
Lysakova-Devine et al., J Immunol 2010 : VIPER was TLR4 specific, being inert toward other TLR pathways, and was active in murine and human cells and in vivo, where it inhibited LPS induced IL-12p40 secretion
Ryan et al., Vet Immunol Immunopathol 2010 (Actinomycetales Infections...) : On day 12, TNF-a induction in monocyte derived macrophages and IL-12 p40 induction in BAL macrophages infected with R. equi was significantly higher in foals treated with PPVO than in controls ... On day 12, TNF-a induction in monocyte derived macrophages and IL-12 p40 induction in BAL macrophages infected with R. equi was significantly higher in foals treated with PPVO than in controls
Scott et al., Am J Reprod Immunol 2011 (Disease Models, Animal...) : Increased expression of IL-6 and IL-12p35 and decreased expression of IL-10 occurred in FIV infected tissues at early pregnancy ; at late gestation, IL-6 expression increased and IL-1ß and SDF-1a decreased
Jones et al., J Immunol 2010 : We compared the ability of progesterone to modulate murine bone marrow derived DC cytokine production ( IL-6 and IL-12 ) and costimulatory molecule expression ( CD40, CD80, and CD86 ) induced by either TLR3 or TLR4 ligation and determined whether activity was via the progesterone receptor (PR) or glucocorticoid receptor ( GR ) by comparative studies with the PR-specific agonist norgestrel and the GR agonist dexamethasone ... We compared the ability of progesterone to modulate murine bone marrow derived DC cytokine production ( IL-6 and IL-12 ) and costimulatory molecule expression ( CD40, CD80, and CD86 ) induced by either TLR3 or TLR4 ligation and determined whether activity was via the progesterone receptor (PR) or glucocorticoid receptor ( GR ) by comparative studies with the PR-specific agonist norgestrel and the GR agonist dexamethasone
de Kivit et al., Immunobiology 2011 (Inflammation) : TLR4 and TLR9 ligands reduced IL-13 secretion in presence and absence of apically exposed IEC and enhanced IL-12 secretion in presence of IEC ... TLR4 and TLR9 ligands reduced IL-13 secretion in presence and absence of apically exposed IEC and enhanced IL-12 secretion in presence of IEC
Kucera et al., J Mol Biol 2010 : Substitution of the acidic loop in TgPRF with the homologous loop from the apicomplexan parasite Cryptosporidium parvum does not affect TLR11 dependent IL-12 secretion, while substitution with the acidic loop from Plasmodium falciparum results in reduced but significant IL-12 secretion
Rastogi et al., Am J Respir Crit Care Med 2011 (Sarcoidosis, Pulmonary) : Inhibition of p38, but not ERK , attenuated production of both IL12/IL23p40 and TNF-a ... Inhibition of p38 , but not ERK, attenuated production of both IL12/IL23p40 and TNF-a
Yang et al., J Immunol 2010 (Leishmaniasis) : We show that inhibition of p38 dramatically increased IL-12 production upon stimulation, while decreasing TNF-a
Tanaka et al., Cell Immunol 2011 : The extract of Japanese soybean, Kurosengoku activates the production of IL-12 and IFN-? by DC or NK1.1 ( + ) cells in a TLR4- and TLR2 dependent manner
Sun et al., Parasite Immunol 2010 (Arthritis, Experimental) : IL-12p70 released from rSj16 treated BMDC was decreased but IL-10 increased
Abdi et al., Scand J Immunol 2010 : Moreover, antigen activated CD4 ( + ) T cells from wild type or IFN-?-deficient mice both initiated IL-12p75 production from DCs
Ueda et al., Int Immunol 2010 : Colonic DCs ( LDC ) showed LPS induced production of IL-12p40
Nguyen et al., J Cell Mol Med 2011 : After maturation with a cytokine cocktail consisting of tumour necrosis factor-a, interleukin (IL)-1ß, IL-6 and prostaglandin ( PG ) E ( 2 ) LC-like cells released IL-12p70 in response to CD40 ligation
Al-Mutairi et al., PLoS Pathog 2010 (Leishmaniasis, Cutaneous) : MKP-2 deletion also potentiated LPS stimulated induction of the inflammatory cytokines, IL-6, IL-12p40 , TNF-a, and also COX-2 derived PGE ( 2 ) production
Ma et al., Immunology 2011 (Hepatitis C, Chronic) : To determine whether PD-1 regulates IL-12 production by M/Mf during chronic HCV infection, we examined the expressions of PD-1, its ligand PDL-1, and their relationship with IL-12 production in M/Mf from HCV infected, HCV resolved, and healthy subjects by flow cytometry
Pifer et al., J Biol Chem 2011 (Toxoplasmosis, Animal) : A deficiency in functional UNC93B1 protein abolished TLR11 dependent IL-12 secretion by dendritic cells, attenuated Th1 responses against T. gondii, and dramatically enhanced susceptibility to the parasite
Song et al., Leukemia 2011 : LBH589 also significantly repressed the production of interleukin (IL)-6, IL-10, IL-12p70 , IL-23 and tumor necrosis factor-a by Toll-like receptor (TLR)3 and TLR4 induced DC activation, indicating an important role of HDAC activity in immune regulation and inflammation ... LBH589 also significantly repressed the production of interleukin (IL)-6, IL-10, IL-12p70 , IL-23 and tumor necrosis factor-a by Toll-like receptor (TLR)3 and TLR4 induced DC activation , indicating an important role of HDAC activity in immune regulation and inflammation
Okazaki et al., J Biochem 2011 : C5a controls TLR induced IL-10 and IL-12 production independent of phosphoinositide 3-kinase ... Mouse macrophages produce IL-12 and IL-10 in response to TLR ligands such as LPS, CpG, Poly I:C and Malp2 ... The TLR induced IL-12 production was decreased, while that of IL-10 was increased by concurrent stimulation with a complement fragment C5a ... Pharmacological studies have suggested that C5a regulates TLR4 induced IL-12 production in a phosphoinositide 3-kinase (PI3K) dependent mechanism
DeBenedette et al., J Immunother 2011 : In the presence of IL-12 , CTL differentiation toward lytic function is not accompanied by a reduction in the secretion of interferon-? and tumor necrosis factor-a
Zenhom et al., Immunobiology 2011 (Inflammation) : Overexpression of PGlyRP3 in Caco2 cells down regulated the expression of the inflammatory cytokines IL-8, IL-12 and TNF-a, while its silencing increased the expression of these cytokines
Gulliver et al., Am J Respir Cell Mol Biol 2011 (Asthma) : The B(1)R agonist Lda-BK was not chemotactic for hMo-DCs matured using LPS, TNF-a + IL-1ß, or CD40 ligand, but Lda-BK enhanced the secretion of IL-12p70 and inhibited the secretion of IL-12p40 by mature hMo-DCs ... The B(1)R agonist Lda-BK was not chemotactic for hMo-DCs matured using LPS, TNF-a + IL-1ß, or CD40 ligand, but Lda-BK enhanced the secretion of IL-12p70 and inhibited the secretion of IL-12p40 by mature hMo-DCs
Setiyono et al., BMC immunology 2011 : Finally, both FL-AFP and D-AFP impaired the MDDC ability to secrete IL-12 ( p70 ) ... Finally, both FL-AFP and D-AFP impaired the MDDC ability to secrete IL-12 ( p70 )
Rivera et al., J Exp Med 2011 (Aspergillosis) : Dectin-1 signaling in A. fumigatus infected wild-type mice reduces IFN-? and IL-12p40 expression in the lung, thereby decreasing T-bet expression in responding CD4 T cells and enhancing Th17 responses
Zhang et al., J Immunol 2011 (Hepatitis C, Chronic) : Blocking gC1qR rescued HCV core induced PD-1 upregulation and IL-12 suppression, whereas blocking PD-1 signaling enhanced IL-12 production and decreased the expression of SOCS-1 induced by HCV core ... Conversely, silencing SOCS-1 expression using small interfering RNAs increased IL-12 expression and inhibited PD-1 upregulation ... Conversely, silencing SOCS-1 expression using small interfering RNAs increased IL-12 expression and inhibited PD-1 upregulation ... PD-1 and SOCS-1 were found to associate by coimmunoprecipitation studies, and blocking PD-1 or silencing SOCS-1 in M/M ( F ) led to activation of STAT-1 during TLR stimulated IL-12 production
Vanbervliet et al., J Allergy Clin Immunol 2011 (Dermatitis, Atopic...) : Lack of H1R expression on dendritic cells (DCs) led to diminished IL-12 , upregulated IL-23, and IL-6 production upon allergen stimulation
Oliveira et al., J Biol Chem 2011 : R. sanguineus tick saliva inhibited IL-12p40 and TNF-a while potentiating IL-10 cytokine production by bone marrow derived DCs stimulated by Toll-like receptor-2, -4, and -9 agonists
Mohamadzadeh et al., Proc Natl Acad Sci U S A 2011 (Colitis) : The data show that the L. acidophilus LTA negative in LTA ( NCK2025 ) not only down-regulated IL-12 and TNFa but also significantly enhanced IL-10 in DCs and controlled the regulation of costimulatory DC functions, resulting in their inability to induce CD4 ( + ) T-cell activation
Ferret-Bernard et al., Vet Res 2011 : The IL-15 increases IL-12 production by an amplifying feedback loop involving CD40
Zwiers et al., J Immunol 2011 (Colitis...) : In addition, this region determines strain differences in LPS induced IL-12 responses
Block et al., Clin Cancer Res 2011 (Mammary Neoplasms, Experimental...) : Human but not mouse APCs upregulate VEGFR2 and downregulate interleukin (IL)-12ß in response to VEGF
Lu et al., Blood 2011 : miR-221 and miR-155 regulate human dendritic cell development, apoptosis, and IL-12 production through targeting of p27kip1, KPC1, and SOCS-1 ... Silencing of miR-155 expression had little effect on DC maturation but reduced IL-12p70 production, whereas miR-155 overexpression in mDCs enhanced IL-12p70 production ... Thus, our study uncovered miRNA signatures during monocyte differentiation into DCs and the new regulatory role of miR-221 and miR-155 in DC apoptosis and IL-12p70 production
McClymont et al., J Immunol 2011 (Diabetes Mellitus, Type 1) : Naive Tregs were capable of upregulating expression of Th1 associated T-bet, CXCR3 , and IFN-? in response to IL-12 ... Naive Tregs were capable of upregulating expression of Th1 associated T-bet, CXCR3 , and IFN-? in response to IL-12
Kasai et al., J Med Invest 2011 (Bone Diseases, Metabolic) : In ex vivo cultures, raloxifene therapy inhibited LPS stimulated production of IL-1ß, IL-6, IL-12p40, IL-12p70 and TNF-a, but not PHA stimulated production of IL-4 and IFN-? ... In in vitro cultures, raloxifene at a concentration ( 10 ( -9 ) M ) inhibited LPS stimulated production of IL-1ß, IL-6 and IL-12p40 and PHA stimulated production of IFN-?
Güttsches et al., Innate Immun 2012 : However, when comparing LPS with lysate patterns, pro-inflammatory cytokine IL-12p40 was up-regulated by both LPS molecules and anti-inflammatory IL-10 by both lysates
Martins et al., J Leukoc Biol 2011 : However, in G-CSFR-deficient DCs or after antibody mediated neutralization of G-CSF, GR-1 CM induced IL-12/23 p40 production significantly, indicating that G-CSF within the GR-1 CM inhibits IL-12/23 p40 production induced by other CM components ... GR-1 CM and rG-CSF also inhibited LPS induced IL-12 production at the mRNA and protein levels ... GR-1 CM and rG-CSF also inhibited LPS induced IL-12 production at the mRNA and protein levels ... Altogether, these results suggest that G-CSF modulates the IL-12/23 p40 response of DCs in the context of the probiotic GR-1 through MF-DC crosstalk
Zenewicz et al., Int Immunol 2011 (Communicable Diseases) : NK cells produce IL-22 in response to IL-12 and IL-18 or IL-23
Godefroy et al., Cancer Cell 2011 (Melanoma) : Furthermore, MMP-2 conditioned dendritic cells (DCs) primed naïve CD4 ( + ) T cells to differentiate into an inflammatory T ( H ) 2 phenotype through OX40L expression and inhibition of IL-12p70 production
Nedjai et al., Cell Immunol 2011 (Hereditary Autoinflammatory Diseases) : Interestingly, high p65 activity was associated with elevated IL-8 secretion, whereas high c-Rel activity increased IL-1ß and IL-12 secretion
Lisiero et al., J Immunol 2011 (Disease Models, Animal...) : Control of tumor growth by CD8 ( + ) T cells was dependent on IL-12 mediated upregulation of the high-affinity IL-2R ( CD25 ) and a subsequent increase in the sensitivity to IL-2 stimulation
Schröder et al., Mol Immunol 2011 : To further characterize SphK1 dependent IL-12p70 regulation we exogenously applied S1P, SEW2871 and the new potent S1P1 agonist CYM5442
Nair et al., J Immunol 2011 (Inflammation...) : In this study, we demonstrate that the PPE18 protein of M. tuberculosis inhibits LPS induced IL-12 and TNF-a production by blocking nuclear translocation of p50, p65 NF-?B, and c-rel transcription factors
Rakshit et al., Int J Cancer 2012 (Neoplasms, Experimental) : In fact, several MIP mediated anti-tumor responses are greatly abrogated in Ifn? ( -/- ) mice : increase in serum Interleukin (IL)12p70 amounts, induction of IL2 and lysis of EL4 targets by splenocytes upon stimulation with specific tumor antigens
Hu et al., Mol Med Report 2008 : SOCS1 silencing in DCs could prevent immune tolerance by inhibiting Fas and Fas-L expression, induced by an increase in IL-12p70 and IL-6 production ... SOCS1 silencing in DCs could prevent immune tolerance by inhibiting Fas and Fas-L expression, induced by an increase in IL-12p70 and IL-6 production
Ramer-Tait et al., Cell Immunol 2011 (Leishmaniasis, Cutaneous) : Neutralization of IL-2 enhanced CD4 ( + ) T cell proliferation in response to IL-12
Kim et al., Biomarker insights 2011 : These results suggest that URC modulates DC function in a fashion that favors Th1 polarization via the activation of IL-12p70 dependent on TLR4 signaling, and may be used on DC-based vaccine for cancer immunotherapy
Feng et al., J Immunol 2011 (Colitis) : Th17 cells induce colitis and promote Th1 cell responses through IL-17 induction of innate IL-12 and IL-23 production ... IL-17 promoted dendritic cell production of IL-12 and IL-23 ... Collectively, these data indicate that Th17 cells convert to Th1 cells through IL-17 induction of mucosal innate IL-12 and IL-23 production
Reis et al., Brain Behav Immun 2011 (Sleep Deprivation) : Here, we determined whether the 24-h sleep-wake cycle has an impact on : ( i ) the levels of circulating complement factors ; and ( ii ) TLR4 mediated IL-12 production from human IFN-? primed monocytes in the presence or absence of C5a receptor signaling
Tung et al., Arch Pharm Res 2011 : Inhibitory effect of ginsenosides from steamed ginseng-leaves and flowers on the LPS stimulated IL-12 production in bone marrow derived dendritic cells ... Noticeably, ginsenoside Rg ( 6 ) ( 12 ) and ginsenoside F ( 4 ) ( 13 ) exhibited particularly inhibitory effect on LPS induced IL-12 production with the inhibition values of 80 and 82 % ; and ginsenoside ST(1) (4), ginsenoside SL(2) ( 8 ), ginsenoside SL(3) ( 9 ), ginsenoside Rh ( 3 ) ( 14 ), ginsenoside Rk ( 2 ) ( 15 ), and ginsenoside Rs ( 4 ) ( 18 ) showed moderate effects with inhibition rates of 63, 65, 67, 68, 71, 73, and 67 %, respectively
Kramp et al., Clin Exp Immunol 2011 (Tuberculosis) : TNF-a treatment induced a decrease in TNF-a, IL-12p40 and IL-10 mRNA levels in peritoneal cells following PPD stimulation while live M. tuberculosis caused an increase in TNF-a mRNA and a decrease in the IL-10 mRNA expression ... TNF-a treatment induced a decrease in TNF-a, IL-12p40 and IL-10 mRNA levels in peritoneal cells following PPD stimulation while live M. tuberculosis caused an increase in TNF-a mRNA and a decrease in the IL-10 mRNA expression
Mendes et al., Vaccine 2011 (Toxoplasmosis, Animal) : We conclude that protective parasite specific-CD8 ( + ) T cells are elicited by a mechanism that involves MyD88 dependent induction of IL-12
Brigl et al., J Exp Med 2011 : Constitutive expression of high levels of IL-12 receptor by iNKT cells enabled instant IL-12 induced STAT4 activation, demonstrating that among T cells, iNKT cells are uniquely equipped for immediate, cytokine-driven activation
Tang et al., Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2011 : In the presence of pCP , the level of IL-12 in supernate ( 265.68± 16.21 ) ng/L was significantly higher than the negative control ( 168.76±10.01 ) ng/L ( P < 0.05 ) and blank control ( 87±5.79 ) ng/L ( P < 0.05 ) ; after co-incubated with autologous T lymphocytes for 3 days, the level of IFN-? with pCP ( 773.04±32.73 ) mg/L was also significantly higher than the negative control ( 573.59±26.11 ) mg/L ( P < 0.05 ) ans blank control ( 362.81±24.27 ) mg/L ( P < 0.05 )
Cheekatla et al., Med Microbiol Immunol 2012 (Leishmaniasis, Visceral) : We show for the first time that in THP-1 derived macrophages and human monocytes, mTOR inhibition by rapamycin reversed L. donovani induced IL-12 and IL-10 modulation ... Blocking of TLR2 significantly attenuated IL-10 induced by the parasite, but did not affect IL-12 production
Kim et al., Clin Exp Immunol 2011 (Neoplasms, Experimental) : DCs modified with siRNA targeting against IL-10R or IL-10 ( DC/siIL-10R or DC/siIL-10 ) led to up-regulation of major histocompatibility complex ( MHC) class II, CD40 co-stimulatory molecule, and IL-12 proinflammatory cytokine after lipopolysacharide (LPS) stimulation compared to DC/siGFP
Yamamoto et al., Clin Exp Immunol 2011 (Carcinoma, Hepatocellular...) : Adding AFP inhibited the interleukin (IL)-12 production of DCs after stimulation with lipopolysaccharide (LPS) [ Toll-like receptor (TLR)-4 ligand ], or Poly ( I:C ) ( TLR-3 ligand ), but not IL-18 production
Kitaura et al., Calcif Tissue Int 2011 : We have reported that IL-12 and IL-18 induce apoptosis in bone marrow cells treated with TNF-a in vitro and that osteoclastogenesis is inhibited by the interaction of TNF-a induced Fas and the IL-12 induced Fas ligand (FasL)
Dragicević et al., Vojnosanit Pregl 2011 : Stimulation of CD40 on Poly ( I:C ) -treated MoDCs significantly enhanced the production of IL-12 , IL-23 and IL-10
Iglesias-Gato et al., Phytother Res 2012 (Inflammation) : Additionally, Prostasanr reduced the constitutive secretion of monocyte chemotactic protein-1 (MCP-1), the LPS induced secretion of IL-12 and inhibited MCP-1 and granulocyte-macrophage colony stimulating factor ( GM-CSF ) production in the presence of LPS on PC-3 cells
Shweash et al., Mol Immunol 2011 (Leishmaniasis) : These data demonstrate for the first time the role of TLR-4 in mediating the effects of L. mexicana promastigotes on MAP kinase activation, up-regulation of COX-2, iNOS as well as arginase-1 expression in macrophages and further shows that PGE ( 2 ), NO and arginase activity all contribute substantially to the inhibition of host cell IL-12 production
Slight et al., Cytokine 2011 : Induction of IL-12p40 and resulting chemokine responsiveness in DCs is TLR2 dependent and coincides with the uptake of F. tularensis LVS and activation of DCs
Peters et al., J Immunol 2011 : Incubation of bone marrow derived dendritic cells with THP treated fibroblasts enhanced sterile IL-6 production and LPS induced generation of IL-1ß, IL-12 , IL-23, and TNF-a ... Finally, there is a strong synergy between calcium depletion in the ER and sterile IL-6, as well as LPS dependent IL-1ß, IL-12 , IL-23, and TNF-a innate responses, findings that have implications for understanding inflammatory diseases that originate in the ER
Liu et al., Gastroenterology 2011 (Colitis) : Blocking IL-10 receptors increased production of IFN-? and IL-12/23p40 whereas exogenous IL-10 suppressed these cytokines
Melchers et al., Retrovirology 2011 : Dendritic cells secreted IL-6, IL-10 and IL-12 in response to stimulation by SOSIP.R6-IZ-CD40L and were able to activate naïve T cells
Das et al., J Biol Chem 2011 (Substance Withdrawal Syndrome) : This study investigated for the first time the role of glucocorticoids in MW modulation of LPS induced IL-12p40 , a key cytokine playing a pivotal role in immunoprotection ... ERK inhibitors reversed MW and MW plus corticosterone inhibition of LPS induced IL-12p40
Marquis et al., PLoS Genet 2011 (Tuberculosis, Pulmonary) : It is required for ontogeny and maturation of macrophages and dendritic cells, for activation of anti-microbial defenses, and for production of the Th1 polarizing cytokine interleukin-12 (IL-12) in response to interferon gamma ( IFN? ) and protection against infection with Mycobacterium tuberculosis
Seregin et al., PloS one 2011 : For example, IL12 production and the induction of co-stimulatory molecule expression by DCs and IFN? production by NK cells in vivo were significantly increased in rEA treated TRIF-KO mice ... For example, IL12 production and the induction of co-stimulatory molecule expression by DCs and IFN? production by NK cells in vivo were significantly increased in rEA treated TRIF-KO mice
Ganesh et al., PloS one 2011 : Here, we examined the differential effects of IL-12 and IL-1ß on Foxp3 expression in T cells when activated in the presence and absence of DCs ... Exogenous IL-12 abolished, but IL-1ß enhanced , the ability of GM-CSF exposed tolerogenic DCs to promote Foxp3 expression
Turtle et al., Blood 2011 : Costimulation with IL-1ß during TCR ligation markedly increased proinflammatory IL-17 production, while IL-12 induced Tc1-like function and restored the response to TCR ligation without costimulation
Donini et al., Prostate 2012 (Prostatic Neoplasms) : The NADPH oxidase inhibitor diphenyliodonium chloride ( DPI ) and the superoxide anion scavenger superoxide dismutase ( SOD ) reproduced this effect, but did not affect IL-12 secretion ... LNCaP enhance IL-1ß, IL-23, IL-6, and TNF-a secretion by decreasing glucan dependent NADPH oxidase activity, whereas glucan increases IL-12 production through NADPH oxidase unrelated mechanisms ... LNCaP enhance IL-1ß, IL-23 , IL-6, and TNF-a secretion by decreasing glucan dependent NADPH oxidase activity, whereas glucan increases IL-12 production through NADPH oxidase unrelated mechanisms ... LNCaP enhance IL-1ß , IL-23, IL-6, and TNF-a secretion by decreasing glucan dependent NADPH oxidase activity, whereas glucan increases IL-12 production through NADPH oxidase unrelated mechanisms ... LNCaP enhance IL-1ß, IL-23, IL-6 , and TNF-a secretion by decreasing glucan dependent NADPH oxidase activity, whereas glucan increases IL-12 production through NADPH oxidase unrelated mechanisms
Karpuzoglu et al., Immunobiology 2011 (Inflammation) : Exposure of splenocytes to AEBSF for 3h noticeably inhibited the induction of IFN?, IL-12, and IL-12 induced STAT4ß , mRNA expression of T-bet and IL-12Rß2
Zhang et al., J Leukoc Biol 2012 : Blocking Tim-3 signaling or silencing Tim-3 expression led to a significant increase in TLR mediated IL-12 production, as well as a decrease in activation induced up-regulation of the immunoinhibitor, PD-1 ; TNF-a production was not altered significantly, but IL-10 production was increased ... Blocking Tim-3 signaling or silencing Tim-3 expression led to a significant increase in TLR mediated IL-12 production, as well as a decrease in activation induced up-regulation of the immunoinhibitor, PD-1 ; TNF-a production was not altered significantly, but IL-10 production was increased
Ishikawa et al., Nihon Arukoru Yakubutsu Igakkai Zasshi 2011 : In contrast, the induction of IL-10 by LPS is enhanced by ethanol treatment, suggesting that elevated IL-10 may play a role in ethanol induced suppression of IL-12 ... However, ethanol inhibited IL-12 production in LPS stimulated DCs devoid of IL-10 ( IL-10/DC ), suggesting that down-regulation of IL-12 by ethanol is independent of the IL-10 levels ... Alternatively, cytokine signaling studies indicate that IL-12 production by DCs is negatively regulated by PI3K and GSK-3, but positively regulated by p38 MAPK, mTOR, and NF-kappa B
Mentink-Kane et al., Gastroenterology 2011 (Disease Models, Animal...) : IL-10, IL-12p40 , and IL-13Ra2 reduced the production and activity of the profibrotic cytokine IL-13
Kuroda et al., Proc Natl Acad Sci U S A 2011 : At the late effector stage, BATF was induced by IL-12 and required for IL-12 mediated histone acetylation and survival of effector T cells
Fujimoto et al., J Vet Med Sci 2012 : In addition, adenosine and ATP prevented the production of LPS induced IL-6, TNF-a and IL-12 in DH82 cells
Yang et al., Eur J Immunol 2011 : The synergistic effect of IL-33 and IL-12 is partly mediated by Gadd45b
Prochazkova et al., Immunobiology 2012 : On the molecular level, IL-12 inhibited the expression of the lineage specific transcription factors Foxp3 and ROR?t in developing Tregs and Th17 cells, respectively ... On the molecular level, IL-12 inhibited the expression of the lineage specific transcription factors Foxp3 and ROR?t in developing Tregs and Th17 cells, respectively
Díaz-Montero et al., American journal of cancer research 2011 : Here we report a simplified combinatorial approach that involves short activation of CD8 ( + ) T cells in the presence of IL-12 followed by adoptive transfer into tumor bearing animals after a single injection of cyclophosphamide
Butcher et al., PLoS Pathog 2011 : High level IL-12p40 production in dROP16 infection was dependent on the host cell adaptor molecule MyD88 , but surprisingly was independent of any previously recognized T. gondii triggered pathway linking to MyD88 ( TLR2, TLR4, TLR9, TLR11, IL-1ß and IL-18 )
Court et al., PloS one 2011 (Inflammation) : Synthetic PIM ( 1 ) isomer and PIM ( 2 ) mimetic potently inhibit TNF and IL-12 p40 expression induced by TLR2 or TLR4 pathways, but not by TLR9 , in murine macrophages ... PIM ( 1 ) and PIM ( 2 ) analogues inhibited LPS induced TNF release by a CD14 dependent pathway, while IL-12 p40 inhibition was CD14 independent, suggesting that PIMs have multifold inhibitory effects on the TLR4 signalling pathway
Cavalcanti et al., PloS one 2011 (Ileitis...) : Lack of MIF caused a reduction of TNF-a, IL-12 , IFN-? and IL-23 and an increased expression of IL-22 in ileal mucosa
Song et al., BMC complementary and alternative medicine 2011 (Colitis...) : LPS induced IL-12p40 , IL-23p19, IL-6 and IL-1ß mRNA expressions were inhibited by BTE
Lupov et al., Blood 2011 (Lymphoma...) : Signal Transducer and Activator of Transcription 4 ( STAT4 ) is a transcription factor that is activated by IL-12 signaling and promotes Th1-cell differentiation and IFN-? production
Goodyear et al., Infect Immun 2012 (Disease Models, Animal...) : Thus, we conclude that the MyD88 dependent recruitment of inflammatory monocytes and DCs to the lungs and the local production of IL-12 , followed by NK cell production of IFN-?, are the key initial cellular responses required for early protection from B. mallei infection
Inada et al., J Immunotoxicol 2011 : Endogenous COX inhibition with propofol, as well as with the selective COX-2 inhibitor, NS-398, did not affect IL-12 and IL-10 production from DC
Rudnicka et al., Microbiol Immunol 2012 (Helicobacter Infections) : IL-12 significantly enhanced the natural as well as H.p LPS and H.p GE driven cytotoxic capacity of lymphocytes
Wu et al., DNA Cell Biol 2012 : Exposure to VPA significantly repressed the production of interleukin 12 (IL-12) , and tumor necrosis factor a by lipopolysaccharide (LPS) induced macrophage activation, in contrast, promoted IL-10 expression
Xue et al., J Immunol 2011 (Inflammation) : The ectopic expression of the miR-10a precursor inhibited, whereas the miR-10a inhibitor promoted , the expression of IL-12/IL-23p40 in dendritic cells ... Collectively, our data demonstrated that microbiota negatively regulates host miR-10a expression, which may contribute to the maintenance of intestinal homeostasis by targeting IL-12/IL-23p40 expression
Packiriswamy et al., Cell Immunol 2012 (Systemic Inflammatory Response Syndrome) : Our results demonstrate that GRK5 regulates IL-12p40 and G-CSF via a mechanism that is common to both MyD88 and TRIF
Yi et al., J Mol Med (Berl) 2012 : Additionally, absence of SRA/CD204 resulted in significantly elevated IL12p35 expression in DCs upon CD40 ligation plus interferon gamma ( IFN-? ) stimulation
Gopal et al., Eur J Immunol 2012 (Disease Models, Animal...) : The ability of IL-17 to downregulate IL-10 and induce IL-12 production allows the generation of subsequent Th1-cell responses ... The ability of IL-17 to downregulate IL-10 and induce IL-12 production allows the generation of subsequent Th1-cell responses
Belderbos et al., Pediatr Allergy Immunol 2012 (Hypersensitivity) : Of innate immune variables, TLR3 mediated IL-12p70 production was highly associated with environmental exposures ( pets, breastfeeding and mode of delivery ), whereas TLR9 mediated cytokine responses were not associated with any environmental factor
Terrazas et al., International journal of biological sciences 2011 : These findings demonstrate that the MIF induced early DC maturation and IL-12 production mediates resistance to T. cruzi infection, probably by activating the p38 pathway
Kimura et al., Exp Eye Res 2012 : LPS induced the release of interleukin (IL)-6, IL-8, MCP-1, RANTES, IP-10, eotaxin, and IL-12 from corneal fibroblasts
Michielsen et al., PloS one 2011 (Colorectal Neoplasms) : Pre-treatment of immature dendritic cells with VEGF inhibited LPS induced upregulation of CD80 and CD54, while CXCL1 inhibited HLA-DR. Interestingly, treatment of dendritic cells with CCL2, CXCL1, CXCL5 or VEGF significantly suppressed their ability to secrete IL-12p70 in response to LPS
Tamburini et al., J Immunother 2012 : IL-6 inducing whole yeast based immunotherapy directly controls IL-12 dependent CD8 T-cell responses
Mutamba et al., Eur J Immunol 2012 : Furthermore, IL-1F8 stimulated increased CD40 and CD80 expression and IL-18 and IL-12 p70 production by MDDCs, which induced proliferation of IFN-? producing CD3 ( + ) lymphocytes ( indicative of inflammatory Th1 subsets ) ... Furthermore, IL-1F8 stimulated increased CD40 and CD80 expression and IL-18 and IL-12 p70 production by MDDCs, which induced proliferation of IFN-? producing CD3 ( + ) lymphocytes ( indicative of inflammatory Th1 subsets )
Reay et al., Cancer Gene Ther 2012 (Fibrosarcoma) : These results demonstrate that although significant antitumor effects are achieved by intratumoral injection of adenovirus expressing either scIL-23 or IL-12 alone and that IL-23 requires endogenous IL-12 for maximum antitumor benefit, the combined use of these cytokines provides no additive or synergistic effect
Sanchez et al., Vaccine 2012 : We have established a vaccine model system in which the primary CD8 ( + ) T cell response is independent of either IL-12 or type I IFN receptors, but dependent on CD27/CD70 interactions
Qureshi et al., Lipids in health and disease 2011 (Inflammation...) : The microarray DNA analyses, followed by pathway analyses indicated that quercetin or d-tocotrienol inhibit several LPS induced expression of several ageing and pro-inflammatory genes ( IL-1ß, IL-1a, IL-6, TNF-a, IL-12 , iNOS, VCAM1, ICAM1, COX2, IL-1RA, TRAF1 and CD40 )
Mora et al., Cell cycle (Georgetown, Tex.) 2012 : For this purpose, we investigated the effect of bindarit on the LPS induced production of inflammatory cytokines ( MCP-1 and MCPs, IL-12ß/p40 , IL-6 and IL-8/KC ) in both a mouse leukaemic monocyte-macrophage cell line and bone marrow derived macrophages ( BMDM ) ... Bindarit inhibits the LPS induced MCP-1 and IL-12ß/p40 expression without affecting other analyzed cytokines
Smeekens et al., PloS one 2011 (Candidiasis, Chronic Mucocutaneous) : Cloning and transfection experiments demonstrate that mutated STAT1 inhibits IL12R/IL-23R signaling, with hyperphosphorylation of STAT1 as the likely underlying molecular mechanism
Fagundes et al., PLoS Negl Trop Dis 2011 (Dengue...) : Therefore, IL-12/IL-18 induced IFN-? production and consequent NOS2 induction are of major importance to host resistance against DENV infection
Qu et al., Mol Ther 2012 (Neoplasms) : Furthermore, DC.Tbet/IL12 pulsed with tumor antigen derived peptides and injected as a therapy distal to the tumor site prevented tumor growth and activated robust antigen-specific Tc1 responses
Chiumiento et al., Int J Immunopathol Pharmacol 2011 (Disease Models, Animal...) : The neutrophil Activating protein of Helicobacter pylori ( HP-NAP ), that induces IL-12 , and IL-23 expression and shifts to Th1 allergen-specific Th2 cells in vitro was used as an anti-Th2 agent in BALB/c mice infected with T. spiralis
Bustamante et al., Ann N Y Acad Sci 2011 (Genetic Predisposition to Disease...) : The MSMD causing mutations in NEMO selectively affect the CD40 dependent induction of IL-12 in mononuclear cells
Rodríguez-Lecompte et al., J Anim Sci 2012 : Expression of IL-12p35 in the ileum at d 11 was down regulated in T2 and T3 compared with T1 ( P = 0.030 and 0.012, respectively )
Qiu et al., Int Immunopharmacol 2012 : The RelB shRNA-DC produced lower IL-12 and higher IL-10 than mature dendritic cells ( mDCs ) and silencing control DCs
Ji et al., Liver Transpl 2012 (Disease Models, Animal...) : In vitro, cAMP-PKA activation diminished macrophage tumor necrosis factor a, IL-6, and IL-12 in an IL-10 dependent manner and prevented necrosis/apoptosis in primary mouse hepatocyte cultures ... In vitro, cAMP-PKA activation diminished macrophage tumor necrosis factor a, IL-6, and IL-12 in an IL-10 dependent manner and prevented necrosis/apoptosis in primary mouse hepatocyte cultures
Huang et al., Mol Biol Rep 2012 : STAT4 and STAT6 were activated by interleukin (IL)-12 and IL-4 stimulation, which were important for the generation of Th1 and Th2 cells ... STAT4 and STAT6 were activated by interleukin (IL)-12 and IL-4 stimulation, which were important for the generation of Th1 and Th2 cells
Brake et al., Parasites & vectors 2012 (Cattle Diseases...) : SGE also inhibited LPS induced upregulation of TNFa, IFN? and IL-12 cytokines, but did not alter IL-4 or CD40 mRNA expression
Kono et al., Vaccine 2012 (Listeriosis) : However, it has been reported that standard fully mature DCs show reduced ability to produce IL-12p70 upon subsequent interaction with antigen ( Ag ) -specific T cells, limiting their in vivo performance for vaccines
Yin et al., Cancer Lett 2012 (Colonic Neoplasms...) : Mechanistic studies revealed that over-expression of IL-12 reduced the expression of IL-4 and STAT6 in CSCs ... Mechanistic studies revealed that over-expression of IL-12 reduced the expression of IL-4 and STAT6 in CSCs
Ma et al., Blood 2012 : We previously found that IL-12 mediates the differentiation of human CD4 ( + ) T cells to the Tfh lineage, because IL-12 induces naive human CD4 ( + ) T cells to acquire expression of IL-21, BCL6, ICOS, and CXCR5, which typify Tfh cells ... Although STAT1 was dispensable, mutations in IL12RB1, TYK2, or STAT3 compromised IL-12 induced expression of IL-21 by human CD4 ( + ) T cells
Khan et al., Hum Immunol 2012 (Leishmaniasis, Visceral) : Peptide-7 induces IL-10 and increases Leishmania donovani infection in macrophages, whereas peptide-19 induces IL-12 and reduces L. donovani infection
Dong et al., J Immunol 2012 : CD27 stimulation promotes the frequency of IL-7 receptor expressing memory precursors and prevents IL-12 mediated loss of CD8 ( + ) T cell memory in the absence of CD4 ( + ) T cell help
Yang et al., J Clin Invest 2012 (Lymphoma, Follicular) : IL-12 upregulates TIM-3 expression and induces T cell exhaustion in patients with follicular B cell non-Hodgkin lymphoma
Zhao et al., PloS one 2012 : Nfkb1 inhibits LPS induced IFN-ß and IL-12 p40 production in macrophages by distinct mechanisms ... Nfkb1 inhibits LPS induced IFN-ß and IL-12 p40 production in macrophages by distinct mechanisms
Małaczewska et al., Pol J Vet Sci 2011 (Endotoxemia) : Regardless of the period of administration, silver nanoparticles enhanced the production of proinflammatory cytokines and anti-inflammatory cytokine IL-10 , and they inhibited IL-12 p70 levels in response to LPS challenge
Panda et al., J Reprod Immunol 2012 (Pre-Eclampsia) : The expression of TLR induced production of TNF-a, IFN-a, IL-6, and IL-12 were measured by multicolor flow cytometry
Belderbos et al., PloS one 2012 : Suppression by neonatal plasma of TLR4 mediated IL-12p70 production, but not induction of TLR4 mediated IL-10 production, was maintained up to the age of 1 month ... The factor causing inhibition of TLR4 mediated IL-12p70 production by cord blood plasma was resistant to heat inactivation or protein depletion and was independent of IL-10, vitamin D and prostaglandin E2 ... In conclusion, human neonatal plasma contains at least two distinct factors that suppress TLR4 mediated IL-12p70 production or induce IL-10 or production ... In conclusion, human neonatal plasma contains at least two distinct factors that suppress TLR4 mediated IL-12p70 production or induce IL-10 or production
Boonyatecha et al., Mol Immunol 2012 (MAP Kinase Signaling System) : In this study, we investigated the role of Notch signaling in regulating expression of the IL-12/IL-23 subunit, IL-12p40
Józefowski et al., Postepy Hig Med Dosw 2012 (Bacterial Infections) : Using specific receptor ligation with antibodies, we showed that SR-A and MARCO regulate in an opposite manner production of IL-12 in macrophages, the cytokine playing a crucial role in Th1/Th2 polarization of adaptive immune responses
Elloumi et al., PloS one 2012 (Colitis...) : Experiments in BMDMs from IL-10 deficient ( Il10 ( -/- ) ) mice demonstrate that inhibition of IL-12 expression by 11R-VIVIT was independent of IL-10 expression
Visekruna et al., Clin Dev Immunol 2012 : Additionally, c-Rel dependent IL-12 and IL-23 transcription by macrophages and dendritic cells is crucial for T-cell differentiation and effector functions
Kumar et al., Parasite Immunol 2012 : Here, we show that parasite GPIs efficiently activate DCs through TLR2 mediated signalling mechanism and induce the production of TNF-a and IL-12
Chentoufi et al., Viral Immunol 2012 (Herpes Simplex) : Here we show for the first time that this is associated with LAT expression, since compared to LAT? virus : ( 1 ) LAT? virus interfered with expression of MHC class I and the co-stimulatory molecules CD80 and CD86 on the surface of DCs ; ( 2 ) LAT? virus impaired DC production of the proinflammatory cytokines IL-6, IL-12 , and TNF-a ; and ( 3 ) DCs infected in vitro with LAT? virus had significantly reduced the ability to stimulate HSV-specific CD8? T cells
Slavica et al., Scand J Immunol 2012 : CD40L stimulation of CT-pulsed DC induced a modest IL-12p40 production, but not IL-12p70 or IL-23 secretion
Chiu et al., J Biomed Sci 2012 (Disease Models, Animal...) : The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2 mediated IL-12 in a syngeneic rat model
Ji et al., Hepatology 2013 (Liver Diseases...) : In vitro, PACAP treatment not only diminished macrophage tumor necrosis factor alpha/IL-6/IL-12 levels in a PKA dependent manner, but also prevented necrosis and apoptosis in primary mouse hepatocyte cultures
Hadidi et al., Mucosal Immunol 2012 (Intestinal Diseases, Parasitic...) : Myeloid cell-specific expression of Ship1 regulates IL-12 production and immunity to helminth infection ... Here, we identify a critical role for Ship1 in the negative regulation of interleukin (IL)-12/23p40 production by macrophages during infection with the intestinal helminth parasite Trichuris muris
Ichikawa et al., PloS one 2012 : Here, we examine the role of toll-like receptors ( TLR 's ) and reactive oxygen species ( ROS ) in IL-12 production by LAB stimulated peritoneal macrophages ... Our results indicate that a TLR is not necessary for IL-12 induction by LAB, whilst the universal adaptor protein, MyD88, is essential
Jin et al., Immunol Lett 2012 (Hepatitis B) : Then, DCs dependent HBV-specific T cell proliferation and IL-12 secretion were secondly determined by BrdU cell proliferation assay and ELISA, respectively
Huang et al., J Cardiovasc Pharmacol 2012 : OxLDL- and Ang II-induced upregulation of CD83 and secretion of IL-12 were all attenuated by LOX-1 neutralizing antibody
Zaal et al., Immunobiology 2013 : While C5a indeed enhanced cytokine production of immature DCs, the addition of C5a inhibited production of IL-12 , IL-23 and TNFa induced by various TLR ligands such as LPS, R848 and Pam ( 3 ) CSK ( 4 )
Joo et al., Gut and liver 2012 : EGCG inhibited accumulation of LPS induced IL-12p40 , IL-6, MCP-1, ICAM-1, and VCAM-1 mRNA in BMMs. EGCG blocked LPS induced I?Ba degradation and RelA nuclear translocation ... U0126 ( an inhibitor of MEK-1/2 ) suppressed the LPS induced IL-12p40 , IL-6, MCP-1, ICAM-1, and VCAM-1 mRNA accumulation in BMMs
Spear et al., J Immunol 2012 (Neoplasms, Experimental) : T cell IFN-? and GM-CSF activated local, tumor associated macrophages, decreased expression of regulatory factors, increased IL-12p40 production, and augmented Ag processing and presentation by host macrophages to Ag-specific T cells
Zeitvogel et al., Exp Dermatol 2012 : IL-27 acts as a priming signal for IL-23 but not IL-12 production on human antigen presenting cells
Paul et al., Eur J Immunol 2012 (Leishmaniasis, Visceral) : This was associated with the toll-like receptor (TLR)-4 mediated IL-12 induction and inhibition of TLR2 mediated IL-10 production ... This was associated with the toll-like receptor (TLR)-4 mediated IL-12 induction and inhibition of TLR2 mediated IL-10 production
Awoniyi et al., PloS one 2012 (Salmonella Infections) : IL-12 and IL-23 regulate innate and adaptive immunity to microbial pathogens through influencing the expression of IFN-?, IL-17, and IL-22 ... IL-12 and IL-23 regulate innate and adaptive immunity to microbial pathogens through influencing the expression of IFN-?, IL-17 , and IL-22
Zhang et al., Cell Mol Immunol 2012 : Brucella abortus is a Gram negative intracellular bacterium that induces MyD88 dependent IL-12 production in dentritic cells (DCs) and a subsequent protective Th1 immune response ... Previous studies have shown that the Toll-like receptor 2 (TLR2) is required for tumor-necrosis factor (TNF) production, whereas TLR9 is responsible for IL-12 induction in DCs after exposure to heat killed Brucella abortus ( HKBA ) ... Interestingly, although p38 was required for HKBA phagocytosis and phagosome maturation, ERK1/2 did not affect these processes but negatively regulated IL-12 production ... Interestingly, although p38 was required for HKBA phagocytosis and phagosome maturation, ERK1/2 did not affect these processes but negatively regulated IL-12 production ... Furthermore, Ras-guanine nucleotide releasing protein 1 ( RasGRP1 ) mediated the TLR2 induced ERK1/2 activation and inhibition of IL-12p40 production ... Furthermore, Ras-guanine nucleotide releasing protein 1 ( RasGRP1 ) mediated the TLR2 induced ERK1/2 activation and inhibition of IL-12p40 production ... Furthermore, Ras-guanine nucleotide releasing protein 1 ( RasGRP1 ) mediated the TLR2 induced ERK1/2 activation and inhibition of IL-12p40 production
Cook et al., Proc Natl Acad Sci U S A 2012 : We further demonstrate a central role for DC IL-4Ra expression in the optimal induction of IFN? responses in vivo in both Th1 and Th2 settings, through a feedback loop in which IL-4 promotes DC secretion of IL-12
Yokoyama et al., Infect Immun 2012 : The production levels of interleukin 12 (IL-12) from mouse macrophages treated with heat killed Staphylococcus aureus and of tumor necrosis factor alpha ( TNF-a ) from RAW 264.7 cells induced by peptidoglycan or lipopeptide TLR2 ligands were strongly suppressed in the presence of SSL3
Chen et al., J Biol Chem 2012 : In addition, production of proinflammatory cytokines, such as interleukin (IL)-1, IL-12 , IL-6, and interferon ( IFN ) -?, induced by both bacterial infection and lipopolysaccharide (LPS) and monodansylcadaverine treatment, is also decreased in the GRIM19 ( +/- ) macrophages
Michielsen et al., Mol Cancer Ther 2012 (Colorectal Neoplasms) : TCM and BCM inhibited lipopolysaccharide (LPS) induced dendritic cell maturation and IL-12p70 secretion ( P < 0.0001 ), while increasing IL-10 secretion ( P = 0.0033 and 0.0220, respectively )
Koizumi et al., Cell Immunol 2012 (Neoplasms) : ELL induced production of Type-1 cytokines such as IL-12 and TNF-a from bone marrow derived dendritic cells ( BMDCs ) in TLR2- and TLR4 dependent manner and remarkably up-regulated the expression of MHC and co-stimulatory molecules
Liu et al., Eur J Immunol 2012 : Type I and III interferons enhance IL-10R expression on human monocytes and macrophages, resulting in IL-10 mediated suppression of TLR induced IL-12 ... Type I and III interferons enhance IL-10R expression on human monocytes and macrophages, resulting in IL-10 mediated suppression of TLR induced IL-12 ... This indicates that IFN-a increased the sensitivity of monocytes to IL-10, and as a result, TLR induced IL-12p70 by IFN pretreated cells was suppressed ... In summary, we demonstrate that one of the consequences of priming human APCs with IFN is to promote the cells ' sensitivity to IL-10, which leads to the inhibition of TLR induced IL-12p70 production
Movita et al., J Leukoc Biol 2012 : Interestingly, despite expression of TLR mRNA on Kupffer cells, ligation of TLR4, TLR7/8 , and TLR9 resulted in a weak induction of IL-10, low or undetectable levels of IL-12p40 and TNF, and up-regulation of CD40 on the surface ... Interestingly, despite expression of TLR mRNA on Kupffer cells, ligation of TLR4, TLR7/8 , and TLR9 resulted in a weak induction of IL-10, low or undetectable levels of IL-12p40 and TNF, and up-regulation of CD40 on the surface
Villacres et al., J Infect Dis 2012 (HIV Infections...) : Peripheral blood mononuclear cells ( PBMCs ) obtained at baseline were used to measure interleukin 1ß (IL-1ß), interleukin 6 (IL-6), interleukin 10 (IL-10), interleukin 12 (IL-12) , and tumor necrosis factor a (TNF-a) responses to Toll-like receptor (TLR) 3 and TLR4 stimulation ... Peripheral blood mononuclear cells ( PBMCs ) obtained at baseline were used to measure interleukin 1ß (IL-1ß), interleukin 6 (IL-6), interleukin 10 (IL-10), interleukin 12 (IL-12) , and tumor necrosis factor a (TNF-a) responses to Toll-like receptor (TLR) 3 and TLR4 stimulation ... Peripheral blood mononuclear cells ( PBMCs ) obtained at baseline were used to measure interleukin 1ß (IL-1ß), interleukin 6 (IL-6), interleukin 10 (IL-10), interleukin 12 (IL-12) , and tumor necrosis factor a (TNF-a) responses to Toll-like receptor (TLR) 3 and TLR4 stimulation
Kuan et al., Int Immunopharmacol 2012 : IPAF induced gene expression of IL-12 and Th1 assosiated cytokines/chemokines
Gentle et al., J Immunol 2012 : We demonstrate that concurrent Notch and TLR stimulation results in a unique cytokine profile in mouse bone-marrow derived DCs characterized by enhanced IL-10 and IL-2, and reduced IL-12 expression compared with TLR ligation alone ... We demonstrate that concurrent Notch and TLR stimulation results in a unique cytokine profile in mouse bone-marrow derived DCs characterized by enhanced IL-10 and IL-2, and reduced IL-12 expression compared with TLR ligation alone
Giovannini et al., Microb Pathog 2012 (Tuberculosis) : It induced significantly less IFN-?, IL-12 and IL-27 transcription than wt in infected MDM, while it increased the transcription of inducible NOS
Biondo et al., Eur J Immunol 2012 (Candidiasis...) : We found here that TLR7 was partially required for the induction of IL-12 ( IL-12p70 ) by Candida albicans or Saccharomyces cerevisiae
Mori et al., Eur J Immunol 2012 : The vaccine adjuvant alum inhibits IL-12 by promoting PI3 kinase signaling while chitosan does not inhibit IL-12 and enhances Th1 and Th17 responses
da Silva et al., Immunobiology 2013 : Neither IL-18 nor PGE2 alone stimulated IL-12 or IFN-? secretion ... However, PGE2 alone stimulated the secretion of 208±89pgIL-10/ml whereas IL-18 alone did not stimulate the secretion of IL-10, IL-12 , TNF-a or INF-? ... CD40L did not stimulate the secretion of IL-10, IL-12 , TNF-a or IFN-? and did not stimulate migration toward CCL19 or CCL21
Kim et al., J Immunol 2012 (Disease Models, Animal...) : PLC activation enhanced the bactericidal activity and hydrogen peroxide production of mouse neutrophils, and it also enhanced the production of IFN-? and IL-12 while inhibiting proseptic TNF-a and IL-1ß production in cecal ligation and puncture mice ... PLC activation enhanced the bactericidal activity and hydrogen peroxide production of mouse neutrophils, and it also enhanced the production of IFN-? and IL-12 while inhibiting proseptic TNF-a and IL-1ß production in cecal ligation and puncture mice ... PLC activation enhanced the bactericidal activity and hydrogen peroxide production of mouse neutrophils, and it also enhanced the production of IFN-? and IL-12 while inhibiting proseptic TNF-a and IL-1ß production in cecal ligation and puncture mice
Sarma et al., PloS one 2012 : The bi-modular architecture of the signaling pathways endows the system with an inherent plasticity which is further expressed in the skewing of the CD40 induced productions of IL-10 and IL-12 , the respective anti-inflammatory and pro-inflammatory cytokines
Bebien et al., PLoS Pathog 2012 (Streptococcal Infections) : ßh/c mediated activation of p38 MAPK drives expression of the anti-inflammatory and immunosuppressive cytokine IL-10 , and inhibits both IL-12 and NOS2 expression in GBS infected macrophages, which are critical factors in host defense ... ßh/c mediated activation of p38 MAPK drives expression of the anti-inflammatory and immunosuppressive cytokine IL-10, and inhibits both IL-12 and NOS2 expression in GBS infected macrophages, which are critical factors in host defense
Klaas et al., J Immunol 2012 : This was accompanied by a strong reduction in MyD88 dependent secretion of TNF-a, IL-6, IL-12 , and IL-10
Matsuse et al., Allergy & rhinology (Providence, R.I.) 2012 : Dex treatment significantly inhibited lipopolysaccharide (LPS) induced IL-10 and IL-12 production as well as baseline IL-12 production in AS mice ... The Prl did not significantly inhibit LPS induced IL-10 and IL-12 production in AS mice ... The Prl did not significantly inhibit LPS induced IL-10 and IL-12 production in AS mice ... More importantly, Prl significantly increased IL-10 and IL-12 in AS mice after RSV infection
Hildebrand et al., Cell communication and signaling : CCS 2012 : Here we show that the activation of heterotrimeric G proteins through PMT suppresses LPS stimulated IL-12p40 production and eventually impairs the T cell activating ability of LPS treated monocytes ... This inhibition of TLR4 induced IL-12p40 expression is mediated by Gai triggered signalling as well as by Gß? dependent activation of PI3kinase and JNK.Taken together we propose the following model : LPS stimulates TLR4 mediated activation of the NF?B-pathway and thereby the production of TNF-a, IL-6 and IL-12p40 ... This inhibition of TLR4 induced IL-12p40 expression is mediated by Gai triggered signalling as well as by Gß? dependent activation of PI3kinase and JNK.Taken together we propose the following model : LPS stimulates TLR4 mediated activation of the NF?B-pathway and thereby the production of TNF-a, IL-6 and IL-12p40
Kim et al., Biol Pharm Bull 2012 (Dermatitis, Atopic...) : IgE secretion was significantly down-regulated, and this was accompanied by decreased levels of interleukin (IL)-4 and IgG1 and increased serum levels of IL-12p40 and IgG2a in FPG treated animals
McBerry et al., J Immunol 2012 (Disease Models, Animal...) : p38 MAPK activation and IL-12p70 production was more robust from TFF2 ( -/- ) CD8+ DC compared with WT CD8+ DC and treatment of WT DC with rTFF2 suppressed TLR induced IL-12/23p40 production ... p38 MAPK activation and IL-12p70 production was more robust from TFF2 ( -/- ) CD8+ DC compared with WT CD8+ DC and treatment of WT DC with rTFF2 suppressed TLR induced IL-12/23p40 production ... p38 MAPK activation and IL-12p70 production was more robust from TFF2 ( -/- ) CD8+ DC compared with WT CD8+ DC and treatment of WT DC with rTFF2 suppressed TLR induced IL-12/23p40 production ... p38 MAPK activation and IL-12p70 production was more robust from TFF2 ( -/- ) CD8+ DC compared with WT CD8+ DC and treatment of WT DC with rTFF2 suppressed TLR induced IL-12/23p40 production ... p38 MAPK activation and IL-12p70 production was more robust from TFF2 ( -/- ) CD8+ DC compared with WT CD8+ DC and treatment of WT DC with rTFF2 suppressed TLR induced IL-12/23p40 production ... p38 MAPK activation and IL-12p70 production was more robust from TFF2 ( -/- ) CD8+ DC compared with WT CD8+ DC and treatment of WT DC with rTFF2 suppressed TLR induced IL-12/23p40 production
Wang et al., J Immunol 2012 : ESAT-6 inhibited LPS/CD40L induced DC production of IL-12 and enhanced that of IL-23 and IL-1ß, without affecting secretion of TNF-a, IL-6, or IL-8 through specific interaction with immature DCs ... ESAT-6 inhibited LPS/CD40L induced DC production of IL-12 and enhanced that of IL-23 and IL-1ß, without affecting secretion of TNF-a, IL-6, or IL-8 through specific interaction with immature DCs ... ESAT-6 reduced LPS/CD40L stimulated transcription of IL-12p35 and enhanced that of IL-23p19 through inhibition of IFN regulatory factor-1 and upregulation of activating transcription factor-2 and c-Jun, transcriptional regulators of IL-12p35 and IL-23p19, respectively ... ESAT-6 reduced LPS/CD40L stimulated transcription of IL-12p35 and enhanced that of IL-23p19 through inhibition of IFN regulatory factor-1 and upregulation of activating transcription factor-2 and c-Jun , transcriptional regulators of IL-12p35 and IL-23p19, respectively ... ESAT-6 reduced LPS/CD40L stimulated transcription of IL-12p35 and enhanced that of IL-23p19 through inhibition of IFN regulatory factor-1 and upregulation of activating transcription factor-2 and c-Jun, transcriptional regulators of IL-12p35 and IL-23p19, respectively
Huang et al., Sheng Li Xue Bao 2012 : [ Amino acids 395-416 in DNA binding domain of STAT4 is involved in IL-12 induced nuclear import of STAT4 ]
Zhong et al., Blood 2012 (Purpura, Thrombocytopenic, Idiopathic) : Th-1 polarizing cytokine IL-12 , secreted after direct contact of patient T-cell and CD16 ( + ) monocytes, was responsible for the inhibitory effect on Treg and IL-17 ( + ) CD4 ( + ) cell proliferation ... Th-1 polarizing cytokine IL-12 , secreted after direct contact of patient T-cell and CD16 ( + ) monocytes, was responsible for the inhibitory effect on Treg and IL-17 ( + ) CD4 ( + ) cell proliferation
Holl et al., PloS one 2012 : However, the absence of either Plexin-B2 or Plexin-D1 on DCs leads to constitutive expression of IL-12/IL-23p40 ... However, the absence of either Plexin-B2 or Plexin-D1 on DCs leads to constitutive expression of IL-12/IL-23p40
Perez-Aso et al., Inflammation 2013 (Arthritis, Rheumatoid...) : Interestingly, TNF-a , but not CGS21680, dramatically inhibited IL-12 mRNA expression
Chang et al., J Immunol 2012 : In transfection analysis, SENP1 removed SUMO3 from IRF8 and enhanced expression of IL12p40 and other target genes ... In transfection analysis, SENP1 removed SUMO3 from IRF8 and enhanced expression of IL12p40 and other target genes
Lee et al., Mol Cell Biochem 2013 (Carcinoma, Hepatocellular...) : The results revealed that the SJSZ glycoprotein ( 10 mg/kg, BW ) inhibits the phosphorylation of MAPKs and expression of NF-?B, pSTAT6, IL-10, and IL-4 ; and normalizes production of ROS and NO, and expression of iNOS, IL-12 , and IFN-?
Kishida et al., Clin Dev Immunol 2012 (Hepatitis C, Chronic) : IL-15 was increased at the end of induction therapy in both early virologic responders ( EAVRs ) and late virologic responders ( LAVRs ) ; CXCL-8, CXCL-10, and CCL-4 levels were significantly decreased ( P < 0.05 ) in EAVR but not in LAVR during NCT, and IL-12 increased significantly ( P < 0.05 ) and CXCL-8 decreased significantly ( P < 0.05 ) after the end of NCT in EAVR but not in LAVR ... IL-15 was increased at the end of induction therapy in both early virologic responders ( EAVRs ) and late virologic responders ( LAVRs ) ; CXCL-8, CXCL-10 , and CCL-4 levels were significantly decreased ( P < 0.05 ) in EAVR but not in LAVR during NCT, and IL-12 increased significantly ( P < 0.05 ) and CXCL-8 decreased significantly ( P < 0.05 ) after the end of NCT in EAVR but not in LAVR ... IL-15 was increased at the end of induction therapy in both early virologic responders ( EAVRs ) and late virologic responders ( LAVRs ) ; CXCL-8, CXCL-10, and CCL-4 levels were significantly decreased ( P < 0.05 ) in EAVR but not in LAVR during NCT, and IL-12 increased significantly ( P < 0.05 ) and CXCL-8 decreased significantly ( P < 0.05 ) after the end of NCT in EAVR but not in LAVR
Hedl et al., Gastroenterology 2012 : In mouse macrophages, which do not produce IL-1 in response to PRR stimulation alone, addition of exogenous IL-1 reversed the ERK mediated inhibition of IL-12p40
Lin et al., J Immunol 2012 : We found that 3-MA could enhance LPS induced NF-?B activation and production of TNF-a, inducible NO synthase (iNOS), cyclooxygenase-2, IL-1ß, and IL-12
Fu et al., Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2012 : [IL-12 induced expression of TRAIL enhances the cytotoxicity of NK cells against Jurkat cells ] ... IL-12 induced expression of TRAIL on NK cells mediated the cytotoxicity to Jurkat cells
Kim et al., Microb Pathog 2013 : In addition, TLR4 was required for the optimal production of IL-6, TNF-a, and IL-12 in BMDCs in response to A. baumannii
Palamaro et al., Italian journal of pediatrics 2012 (Bronchopneumonia...) : ABSTRACT : Interleukin-12 (IL-12) is involved in cellular immune responses against intracellular pathogens by promoting the generation of T naive in T helper 1 ( Th1 ) cells and by increasing interferon-gamma (IFN-gamma) production from T and natural killer ( NK ) cells
Chen et al., Immunol Lett 2012 (Liver Neoplasms...) : We found that Tregs derived from the tumor mice down-regulated the expression of costimulatory molecules CD80/CD86 on DCs and inhibited the production of TNF-a and IL-12 from DCs ... We found that Tregs derived from the tumor mice down-regulated the expression of costimulatory molecules CD80/CD86 on DCs and inhibited the production of TNF-a and IL-12 from DCs
Chu et al., J Allergy Clin Immunol 2013 (Hypersensitivity) : We showed that experimental HDM induced allergic asthma and food allergy and anaphylaxis to peanut were associated with TSLP production but developed independently of TSLP, likely because these allergens functionally mimicked TSLP inhibition of IL-12 production and induction of OX40 ligand (OX40L) on DCs
Suzuki et al., Int Arch Allergy Immunol 2013 : The secretion of tumor necrosis factor (TNF)-a, interleukin (IL)-1ß, IL-6, IL-8, IL-12 and macrophage inflammatory protein-1ß increased 3- to 10-fold in response to P-selectin compared with unstimulated monocytes
Zhao et al., PloS one 2012 : We further showed that IL-12 increased IL-2R expression on Tconv and CD8 T cells, diminished its expression on Tregs and decreased IL-2 production by Tconv and CD8 T cells
Nelius et al., Prostate 2013 (Prostatic Neoplasms...) : Finally, PEDF stimulated the expression of iNOS, IL12 , and TNFa ; and inhibited IL10 and arginase 1 in mouse and human macrophages confirming a M1-type differentiation ... Finally, PEDF stimulated the expression of iNOS, IL12 , and TNFa ; and inhibited IL10 and arginase 1 in mouse and human macrophages confirming a M1-type differentiation ... Finally, PEDF stimulated the expression of iNOS, IL12 , and TNFa ; and inhibited IL10 and arginase 1 in mouse and human macrophages confirming a M1-type differentiation
Taylor-Fishwick et al., Diabetologia 2013 (Diabetes Mellitus, Type 2) : Functionally, IL-12 impaired glucose stimulated insulin secretion ( GSIS ) in INS-1 cells and human donor islets
Agrawal et al., Aging 2012 (Cytomegalovirus Infections) : Investigation into the signaling mechanism suggested that phosphorylation of STAT-4 in response to IL-12 was sustained for a longer duration in aged CD4+ T cells as compared to CD4+ T cells from young subjects
Zhang et al., Eur J Immunol 2013 : Klf10 inhibits IL-12p40 production in macrophage colony stimulating factor induced mouse bone marrow derived macrophages ... Klf11 , another member of the Krüppel-like factor family, can also repress the production of IL-12p40
Rossato et al., Proc Natl Acad Sci U S A 2012 (Sepsis) : IL-10 induced microRNA-187 negatively regulates TNF-a, IL-6, and IL-12p40 production in TLR4 stimulated monocytes ... Ectopic expression of miR-187 consistently and selectively reduces TNFa, IL-6, and IL-12p40 produced by LPS activated monocytes ... Conversely, the production of LPS induced TNF-a, IL-6, and IL-12p40 is increased significantly when miR-187 expression is silenced
La Flamme et al., PloS one 2012 : Additionally, we discovered that type II macrophages from both strains were capable of producing IL-4 ; however, this IL-4 was not responsible for the reduced IL-12p40 and IL-10 levels produced by WT mice ... Instead, we found that derivation culture conditions ( GM-CSF plus IL-3 versus M-CSF ) could explain the different responses of BALB/c and IL-4Ra-/- macrophages, and these cytokines shaped the ensuing macrophage such that GM-CSF plus IL-3 promoted more IL-12 and IL-4 while M-CSF led to higher IL-10 production ... Instead, we found that derivation culture conditions ( GM-CSF plus IL-3 versus M-CSF ) could explain the different responses of BALB/c and IL-4Ra-/- macrophages, and these cytokines shaped the ensuing macrophage such that GM-CSF plus IL-3 promoted more IL-12 and IL-4 while M-CSF led to higher IL-10 production
Miyazawa et al., J Biochem 2012 : However, Okazaki et al. ( C5a controls TLR induced IL-10 and IL-12 production independent of phosphoinositide 3-kinase ... 2011 ; 149 : 265-274 ) recently indicated that PI3K activated downstream of C5a anaphylatoxin does not affect the induction of IL-12 by TLR , although C5a efficiently inhibits the induction, in macrophages ... 2011 ; 149 : 265-274 ) recently indicated that PI3K activated downstream of C5a anaphylatoxin does not affect the induction of IL-12 by TLR, although C5a efficiently inhibits the induction, in macrophages ... PI3K is thus a context dependent regulator of TLR mediated IL-12 production
Gharavi et al., Iranian journal of public health 2011 : In addition, AGE increased the level of IL-12 in Leishmania infected cell lines significantly ... AGE treated cell is effective against parasitic pathogens, and AGE induced IL-12 differentially affected the immune response to invading Leishmania parasites
Poudel et al., Cell Immunol 2012 (Melanoma, Experimental) : Collagen I enhanced the release of IL-12p40 , TNF-a and IFN-? by hDCs
Rasouli et al., Cytokine 2013 (Brucellosis...) : It is also clarified that IL-17 is required for the induction of IFN-? and IL-12 in macrophages and dendritic cells
Lewkowich et al., Mucosal Immunol 2013 : Decreased AHR was associated with enhanced serum interleukin (IL)-12 p40, and in vitro stimulation of DCs with allergen and PD-L2-Fc reduced IL-12 p70 production, suggesting that PD-L2 inhibits allergen-driven IL-12 production ... Decreased AHR was associated with enhanced serum interleukin (IL)-12 p40, and in vitro stimulation of DCs with allergen and PD-L2-Fc reduced IL-12 p70 production, suggesting that PD-L2 inhibits allergen-driven IL-12 production ... In our model, IL-12 did not diminish T helper type 2 responses but rather directly antagonized IL-13-inducible gene expression, highlighting a novel role for IL-12 in regulation of IL-13 signaling
Matsui et al., Microbiol Immunol 2013 : These results suggest that PEG can induce Th2 development through down-regulation of IL-12 p70 production by LCs in an IL-10 production dependent manner and would explain the role of S. aureus colonization in patients with AD
Ren et al., Zhonghua Gan Zang Bing Za Zhi 2012 : In the mouse model, GSK3b activity was found to promote the gene expression of anti-inflammatory cytokine IL-10 ( control : 0.21+/-0.08, inflammation : 0.83+/-0.21, intervention : 1.76+/-0.67 ; F = 3.16, P = 0.027 ) but to significantly inhibit the gene expression of pro-inflammatory cytokines IL-12 ( control : 0.11+/-0.05, inflammation : 0.85+/-0.11, intervention : 0.43+/-0.10 ; F = 2.67, P = 0.038 ), TNF-a, ( control : 0.052+/-0.012, inflammation : 8.11+/-0.98, intervention : 3.9+/-0.82 ; F = 4.13, P = 0.016 ), IL-6 ( control : 0.22+/-0.08, inflammation : 6.37+/-0.81, intervention : 2.11+/-0.63 ; F = 3.21, P = 0.024 ), and IL-1b ( control : 0.12+/-0.07, inflammation : 2.51+/-0.62, and intervention : 1.28+/- 0.33 ; F = 2.22, P = 0.030 )
Cargnelutti et al., Immunol Cell Biol 2013 : In addition, we found that CD4 ( + ) T cells from TNFRp55 ( -/- ) mice controlled antigen-specific IL-12/23 ( p40 ) production in recipient WT mice
Negrini et al., Immunol Invest 2013 (Sporotrichosis) : The absence of TLR-2 led to lower production of the cytokines TNF-a, IL-1ß, IL-12 and IL-10 compared to WT animals
Koblansky et al., Immunity 2013 (Genetic Predisposition to Disease...) : TLR12 dependent induction of IL-12 and IFN-a in pDCs led to production of IFN-? by NK cells
Hotho et al., Antiviral Res 2013 (Hepatitis C, Chronic...) : Also, IL-12/18 induced IFN-gamma production by NK cells was comparable between all groups, whereas the cytotoxic ability of NK cells ( granzyme and CD107a levels ) was more potent in HCV infected patients as compared to healthy controls, and highest in non-substance users
Hwang et al., PloS one 2012 (Influenza, Human) : Analysis of cytokine receptor deficient mice demonstrated that type I interferons ( IFNs ), but not IL-12 or IL-18, were critical for the NK cell expression of both IFN-? and granzyme B in response to flu infection
Katholnig et al., J Immunol 2013 : p38a signaling in myeloid immune cells promoted IL-10 but inhibited IL-12 expression via mTOR and blocked the differentiation of proinflammatory CD4 ( + ) Th1 cells ... p38a signaling in myeloid immune cells promoted IL-10 but inhibited IL-12 expression via mTOR and blocked the differentiation of proinflammatory CD4 ( + ) Th1 cells
Hémont et al., J Leukoc Biol 2013 : In contrast, with the use of a whole blood assay, we found that CD141 ( + ) DCs produce IL-12 in response to TLR1/2 , -3, and more surprisingly, -9
Lecocq et al., J Immunol 2013 : These data indicate that FcaRI ligation on human monocytes and dendritic cells inhibits IL-12 expression and type 1 activation by interfering with STAT1 activation
Feriotti et al., PloS one 2013 (Disease Susceptibility...) : Importantly, both mannan and P. brasiliensis induced the production of IL-12 by B10.A macrophages, whereas TGF-ß, TNF-a and IL-6 were produced by A/J cells ... Importantly, both mannan and P. brasiliensis induced the production of IL-12 by B10.A macrophages, whereas TGF-ß, TNF-a and IL-6 were produced by A/J cells
Wang et al., J Virol 2013 (Hepatitis C, Chronic) : In this report, we studied T cell immunoglobulin mucin domain protein 3 ( Tim-3 ) expression on monocytes and its regulatory effect on interleukin-12 (IL-12)/IL-23 production by CD14 ( + ) monocytes, as well as IL-17 production by CD4 ( + ) T cells in individuals with chronic hepatitis C virus ( HCV ) infection ... We found that Tim-3 and IL-23p19 are highly expressed and that IL-12p35 is inhibited in human CD14 ( + ) monocytes, while IL-17 expression is upregulated in CD4 ( + ) T cells, in chronically HCV infected individuals compared to healthy subjects ... We found that Tim-3 and IL-23p19 are highly expressed and that IL-12p35 is inhibited in human CD14 ( + ) monocytes, while IL-17 expression is upregulated in CD4 ( + ) T cells, in chronically HCV infected individuals compared to healthy subjects ... Our findings suggest that Tim-3 mediated differential regulation of IL-12/IL-23 drives TH17 cell development, a milieu favoring viral persistence and autoimmune phenomenon during HCV infection
Thakur et al., Journal of translational medicine 2013 (Pancreatic Neoplasms) : Reduced accumulation of MDSC was accompanied by significantly lower levels of COX2 ( p < 0.0048 ), PGE2 ( p < 0.03 ), and their downstream effector molecule Arginase-1 ( p < 0.01 ), and significantly higher levels of TNF-a, IL-12 and chemokines CCL3, CCL4, CCL5, CXCL9 and CXCL10 under aATC induced Th1 cytokine enriched microenvironment ... Reduced accumulation of MDSC was accompanied by significantly lower levels of COX2 ( p < 0.0048 ), PGE2 ( p < 0.03 ), and their downstream effector molecule Arginase-1 ( p < 0.01 ), and significantly higher levels of TNF-a, IL-12 and chemokines CCL3, CCL4, CCL5, CXCL9 and CXCL10 under aATC induced Th1 cytokine enriched microenvironment
Jamontt et al., J Immunol 2013 (Chronic Disease...) : However, loss of NOD2 in IL-10-deficient macrophages reduced IL-6, TNF-a, and IL-12p40 production in response to bacterial stimulation
Sato et al., Inflammation 2013 : When human PBMCs were stimulated in vitro with penicillin treated Streptococcus pyogenes, bacterial DNA motifs and lipopolysaccharide with or without synthetic CRP, CRP suppressed the production of TNF-a and IL-12 , but not that of IFN-?
Mohanram et al., J Immunol 2013 : Conversely, low quantities of IFN-a failed to upregulate costimulatory molecules, did not induce IL-12p40 or migration, but significantly induced A3G, A3A , and A3F mRNA expression and restricted viral replication in MDDCs ... Conversely, low quantities of IFN-a failed to upregulate costimulatory molecules, did not induce IL-12p40 or migration, but significantly induced A3G, A3A, and A3F mRNA expression and restricted viral replication in MDDCs ... Conversely, low quantities of IFN-a failed to upregulate costimulatory molecules, did not induce IL-12p40 or migration, but significantly induced A3G , A3A, and A3F mRNA expression and restricted viral replication in MDDCs
Jeoung et al., BMC complementary and alternative medicine 2013 (Arthritis, Rheumatoid) : GHJTY inhibited IL-1ß mediated NF-?B activation in HIG-82 cells and reduced the LPS mediated production of proinflammatory cytokines, TNF-a, IL-12 , and NO in RAW264.7 cells
Masui et al., Rheumatology (Oxford) 2013 (Disease Progression...) : In in vitro experiments, visfatin reversed the pro-fibrotic phenotype of SSc dermal fibroblasts and induced the expression of IL-12p70 in THP-1 cells treated with IFN-? plus lipopolysaccharide
Souza-Fonseca-Guimaraes et al., J Biol Chem 2013 (MAP Kinase Signaling System) : Using knock-out-mice, we show that UNC93b1 and IL-12 ( produced by NK cells themselves ) are also necessary for IFN-? and GM-CSF production
de Almeida et al., Infect Immun 2013 (Brucellosis...) : Additionally, B. abortus infected dendritic cells require TLR6 to induce tumor necrosis factor alpha ( TNF-a ) and interleukin-12 (IL-12)
Ma et al., J Surg Res 2013 : T cells pre sensitized by LPS stimulated imDCs showed remarkably elevated proliferation in response to T cells from either BALB/c or C57BL/6 mice ( P < 0.01 ). We observed a significant decrease in the proliferation of T cells pre sensitized by stimulated imDCs after CD1d blockade. Lipopolysaccharide stimulation caused elevated the production of IL-12 and tumor necrosis factor-a (TNF-a) ( P < 0.01 ) and decreased the secretion of IL-10 ( P < 0.05 ). The addition of CD1d neutralization antibody did not significantly change the concentrations of IL-12, TNF-a, or IL-10 produced by imDCs cultured in the presence of LPS ( P > 0.05 )
Rahim et al., J Immunol 2013 (Melanoma, Experimental) : Furthermore, Ly49Q expression on pDC, but not myeloid dendritic cells, is necessary for optimal IL-12 secretion, MHC-II expression, activation of CD4 ( + ) T cell proliferation, and nuclear translocation of the master IFN-a regulator IFN regulatory factor 7 in response to TLR9 agonists ... Furthermore, Ly49Q expression on pDC, but not myeloid dendritic cells, is necessary for optimal IL-12 secretion, MHC-II expression, activation of CD4 ( + ) T cell proliferation, and nuclear translocation of the master IFN-a regulator IFN regulatory factor 7 in response to TLR9 agonists
Kobayashi et al., Proc Natl Acad Sci U S A 2013 (Shock, Septic) : LPS induced TNF-a and IL-12 production in DCs and Ms was significantly enhanced in BTLA ( -/- ) mice
Ferrante et al., Inflammation 2013 : Adenosine signaling suppresses the TLR dependent expression of TNF-a, IL-12 , IFN-?, and several other inflammatory cytokines by macrophages and induces the expression of vascular endothelial growth factor ( VEGF ) and IL-10 ... Adenosine signaling suppresses the TLR dependent expression of TNF-a, IL-12 , IFN-?, and several other inflammatory cytokines by macrophages and induces the expression of vascular endothelial growth factor ( VEGF ) and IL-10 ... Adenosine signaling suppresses the TLR dependent expression of TNF-a, IL-12 , IFN-?, and several other inflammatory cytokines by macrophages and induces the expression of vascular endothelial growth factor ( VEGF ) and IL-10
Liu et al., Scand J Immunol 2013 : Our results demonstrate that not only TLR2 but also TLR4 and RP105 are involved in the response of macrophages to S. aureus, that TLR2, TLR4 and RP105 physically interact with each other during S. aureus infection, and that TLR2 , TLR4 and RP105 both cooperate and play unique roles in the production of inflammatory cytokines ( TNF-a, IL-12p40 and IL-10 ) and chemokine ( RANTES ) by macrophages after S. aureus infection ... Our results demonstrate that not only TLR2 but also TLR4 and RP105 are involved in the response of macrophages to S. aureus, that TLR2, TLR4 and RP105 physically interact with each other during S. aureus infection, and that TLR2, TLR4 and RP105 both cooperate and play unique roles in the production of inflammatory cytokines ( TNF-a, IL-12p40 and IL-10 ) and chemokine ( RANTES ) by macrophages after S. aureus infection
Ma et al., PloS one 2013 : Involvement of DNA-PKcs in the IL-6 and IL-12 Response to CpG-ODN Is Mediated by Its Interaction with TRAF6 in Dendritic Cells
Elcombe et al., PloS one 2013 : We further show that the reduction in IL-10 production in the MSK1/2 macrophages results in increased secretion of IL-12p40 in response to zymosan relative to wild type controls
Kaiko et al., J Allergy Clin Immunol 2013 (Asthma...) : TLR7 deficiency increased viral load in the airway epithelium, which became sloughed and necrotic, and promoted an IFN-a/ß ( low ), IL-12p70 ( low ), IL-1ß ( high ), IL-25 ( high ), and IL-33 ( high ) cytokine microenvironment that was associated with the recruitment of type 2 innate lymphoid cells/nuocytes and increased TH2-type cytokine production
Kerkar et al., Mol Ther 2013 (Melanoma, Experimental) : Collapse of the Tumor Stroma is Triggered by IL-12 Induction of Fas ... Taken together, these results describe the likely requirement for costimulatory reverse signaling through Fasl on T cells that successfully infiltrate tumors, a mechanism triggered by the induction of Fas expression on myeloid derived cells by IL-12 and the subsequent collapse of the tumor stroma
Takashima et al., Blood 2013 : In this issue of Blood, Janelsins et al report that substance P ( SP ) -treated dendritic cells (DCs) efficiently home to lymph nodes, where they induce inflammatory DCs to produce interleukin-12 (IL-12) , thereby promoting type 1 polarized immunity
You et al., Immunobiology 2013 : In the MLR, DCs from older subjects induced significantly restricted proliferation of young T cells, activation of CD8 ( + ) T cells and expression of IL-12 and IFN-? in T cells compared with young DCs
Poudel et al., Biochem Biophys Res Commun 2013 : We investigated the mechanism involving discoidin domain receptor 2 (DDR2) mediated production of interleukin 12 (IL-12) ... In summary, DDR2 is involved in the collagen I-induced IL-12 production via NF-?B and JNK pathway
Wang et al., J Immunol 2013 (Hepatitis B, Chronic) : Moreover, the inhibition of JNK phosphorylation and IL-12 production in response to Pam3csk was observed in HBsAg treated monocytes/macrophages ( M/MFs ) from the healthy donors and the PBMCs and CD14 positive M/MFs from chronic hepatitis B patients
Kang et al., Life Sci 2013 (Colitis...) : GG-52 significantly inhibited LPS induced IL-12p40 and TNF-a gene expression, I?Ba degradation, and NF-?B DNA binding activity in BMDCs
Patel et al., Biometals 2013 : Neocuproine, but not ATP7A deficiency, reduced the production of FGF-9, IL-1a, IL-12p70 , IL-2, IL-3, IL-4, IL-6, MIP-1ß, MIP-2, RANTES, and TNFa
Burnham et al., Am J Physiol Lung Cell Mol Physiol 2013 (Alcoholism) : Cigarette smoking duration was associated with higher IL-1ß ( P = 0.0009 ) but lower VEGF ( P = 0.0007 ) ; cigarette smoking intensity was characterized by higher IL-1ß and lower VEGF and diminished IL-12 ( P = 0.0004 ) ... Cigarette smoking duration was associated with higher IL-1ß ( P = 0.0009 ) but lower VEGF ( P = 0.0007 ) ; cigarette smoking intensity was characterized by higher IL-1ß and lower VEGF and diminished IL-12 ( P = 0.0004 )
Wei et al., Neurobiol Dis 2013 : We observed that the absence of apoE resulted in the increased proportion of Th1 and Th17 cells in the spleens and brains, as well as up-regulated expressions of proinflammatory cytokines ( IL-17, IFN-?, TNF-a, IL-12 , IL-1ß and IL-6 ) and transcription factors ( ROR?t and T-bet ) in the CNS
Kasraie et al., Allergy 2013 : IL-12p40 secretion via ERK 1/2 phosphorylation in human macrophages following up-regulation of IL-31 receptor with staphylococcal exotoxins
Hoffmann et al., PloS one 2013 (Colitis...) : While RA impeded the induction of fucosyltransferase-VII it did not affect IL-12 dependent C2GlcNAcT-I induction suggesting that C2GlcNAcT-I can support P-lig expression even if fucosyltransferase-VII mRNA upregulation is dampened
Gasteiger et al., J Exp Med 2013 : Here, we report that T reg cells restrained the expansion of immature CD127 ( + ) NK cells, which had the unique ability to up-regulate the IL2Ra ( CD25 ) in response to the proinflammatory cytokine IL-12
Nolte et al., Eur J Immunol 2013 : With ( out ) a little help from my friends : an IL-12/CD40L mediated feed-forward loop between CD8+ T cells and DCs ... 2013. 43 : 1511-1517 ] show that CD40L expression on these cells can be part of a self sustaining feed-forward loop, in which expression of CD40L is induced by IL-12 and TCR signaling
Orr et al., PLoS Pathog 2013 (Candidiasis...) : LAB/NTAL Facilitates Fungal/PAMP induced IL-12 and IFN-? Production by Repressing ß-Catenin Activation in Dendritic Cells ... C. albicans- and LPS induced IL-12 and IL-23 production was blunted in Lat2 ( -/- ) DCs
Rasmussen et al., J Med Microbiol 2013 (Campylobacter Infections) : Murine dendritic cells exposed to UV-killed bacteria showed a reduced IL-12 production, but the same IL-10 response when encountering C. jejuni dcstA compared to the WT strain
Mussá et al., Vet Immunol Immunopathol 2013 : All viruses induced the secretion of IL-12 mostly at 24hpi whereas IL-18 was detected at all tested times
Brosbøl-Ravnborg et al., Clin Dev Immunol 2013 : Vitamin D3 acted in synergy with the TLR agonists LPS and peptidoglycan ( PGN ) in inducing IL-6, IL-8, and IL-10, whereas vitamin D3 completely inhibited LPS induced secretion of IL-12
Zheng et al., PloS one 2013 : In this study, we demonstrate that heat killed C. albicans ( HKC ) strongly suppressed LPS induced IL-12p70 production in M2 macrophages ... The over-expression of EBI3 in the IL-12p70 producing cell line effectively suppressed IL-12p70 production ... Our results have indicated that C. albicans can suppress host inflammatory responses in mucosal skin by suppressing LPS induced IL-12p70 production
Wei et al., J Immunol 2013 (Melanoma, Experimental) : DC-derived IL-27 is required for shaping tumor microenvironment by inducing CXCL-10 expression in myeloid derived suppressor cells and regulating IL-12 production from DCs, which lead to the recruitment and activation of NK and NKT cells resulting in immunological control of tumors
Koopman et al., Clin Exp Immunol 2013 : In contrast to humans, pDC in rhesus macaques expressed the interleukin (IL)-12p40 subunit in response to TLR-7/8 as well as TLR-9 stimulation ... In contrast to humans, pDC in rhesus macaques expressed the interleukin (IL)-12p40 subunit in response to TLR-7/8 as well as TLR-9 stimulation ... In contrast to humans, pDC in rhesus macaques expressed the interleukin (IL)-12p40 subunit in response to TLR-7/8 as well as TLR-9 stimulation ... Both in humans and rhesus macaques, TLR-4 stimulation induced IL-12p40 expression in mDC and monocytes, but not in pDC ... Both in humans and rhesus macaques, TLR-4 stimulation induced IL-12p40 expression in mDC and monocytes, but not in pDC
Compté et al., PloS one 2013 : The aim of our study was to define the relative contribution of age and clinical status on TLR induced interleukin (IL)-12p70 and IL-23 production as these cytokines play an important role in the protection against intracellular and extracellular pathogens, respectively
Brancato et al., Wound Repair Regen 2013 (Disease Progression...) : The accumulation of CCL2, CX3CL1, tumor necrosis factor-a, interleukin (IL)-6, IL-10, IL-12 , and interferon-? in wound fluids was not TLR4 dependent
Kitaura et al., Clin Dev Immunol 2013 (Arthritis, Rheumatoid...) : IL-12, IL-18, and IFN- ? induce apoptosis in bone marrow cells treated with TNF- a ??in vitro, and osteoclastogenesis is inhibited by the interactions of TNF- a -induced Fas and Fas ligand induced by IL-12 , IL-18, and IFN- ? ... IL-12, IL-18, and IFN- ? induce apoptosis in bone marrow cells treated with TNF- a ??in vitro, and osteoclastogenesis is inhibited by the interactions of TNF- a -induced Fas and Fas ligand induced by IL-12 , IL-18, and IFN- ?
Stark et al., Eur J Immunol 2013 : IL-12 mediated STAT4 signaling and TCR signal strength cooperate in the induction of CD40L in human and mouse CD8 ( + ) T cells ... The induction of CD40L in CD8 ( + ) T cells regulated by IL-12 and TCR signaling may enable CD8 ( + ) T cells to respond autonomously of CD4 ( + ) T cells
Fowler et al., Immunol Cell Biol 2013 : We found that Vd2 cells are indirectly activated by BCG in an interleukin (IL)-12p70 dependent manner, and that DC production of the IL-12p70 responsible for Vd2 cell activation requires Toll-like receptor 2/4 ligands from BCG and interferon ( IFN ) -? from memory CD4 T cells ... We found that Vd2 cells are indirectly activated by BCG in an interleukin (IL)-12p70 dependent manner, and that DC production of the IL-12p70 responsible for Vd2 cell activation requires Toll-like receptor 2/4 ligands from BCG and interferon ( IFN ) -? from memory CD4 T cells ... We found that Vd2 cells are indirectly activated by BCG in an interleukin (IL)-12p70 dependent manner, and that DC production of the IL-12p70 responsible for Vd2 cell activation requires Toll-like receptor 2/4 ligands from BCG and interferon ( IFN ) -? from memory CD4 T cells
Wölfl et al., Blood 2013 : Src-kinase inhibitors, and not conventional tyrosine kinase inhibitors, increased IL-12 production in several cell types of myeloid origin, such as monocytes and classical or nonclassical DCs
Carow et al., PLoS Pathog 2013 : Instead, SOCS3 expression in infected macrophages and DCs prevented the IL-6 mediated inhibition of TNF and IL-12 secretion and contributed to a timely CD4+ cell dependent IFN-? expression in vivo ... Instead, SOCS3 expression in infected macrophages and DCs prevented the IL-6 mediated inhibition of TNF and IL-12 secretion and contributed to a timely CD4+ cell dependent IFN-? expression in vivo ... Instead, SOCS3 expression in infected macrophages and DCs prevented the IL-6 mediated inhibition of TNF and IL-12 secretion and contributed to a timely CD4+ cell dependent IFN-? expression in vivo ... Instead, SOCS3 expression in infected macrophages and DCs prevented the IL-6 mediated inhibition of TNF and IL-12 secretion and contributed to a timely CD4+ cell dependent IFN-? expression in vivo
Xue et al., Int Immunopharmacol 2013 : We elucidated that IFN-? up-regulated the expression of MHC II, CD40 , CD80, CD83 and CD86 molecules on BMDCs, down-regulated the activity of pinocytosis and phagocytosis by BMDCs, and induced higher levels of IL-12 and TNF-a secreted by BMDCs ... We elucidated that IFN-? up-regulated the expression of MHC II, CD40, CD80 , CD83 and CD86 molecules on BMDCs, down-regulated the activity of pinocytosis and phagocytosis by BMDCs, and induced higher levels of IL-12 and TNF-a secreted by BMDCs ... We elucidated that IFN-? up-regulated the expression of MHC II, CD40, CD80, CD83 and CD86 molecules on BMDCs, down-regulated the activity of pinocytosis and phagocytosis by BMDCs, and induced higher levels of IL-12 and TNF-a secreted by BMDCs ... We elucidated that IFN-? up-regulated the expression of MHC II, CD40, CD80, CD83 and CD86 molecules on BMDCs, down-regulated the activity of pinocytosis and phagocytosis by BMDCs, and induced higher levels of IL-12 and TNF-a secreted by BMDCs
Sung et al., Biochem Biophys Res Commun 2013 : Lipopolysaccharide (LPS) induced expression of cell surface molecules ( CD80, CD86, and MHC class I/II ) and production of pro-inflammatory cytokines ( tumor necrosis factor-a, IL-1ß, IL-6, and IL-12p70 ) were inhibited by Pro B2, and this action was prevented by IRAK-M silencing
Watanabe et al., PloS one 2013 : LPS induces the production of inflammatory cytokines such as TNF-a, IL-6, and IL-12 from dendritic cells (DCs) and macrophages
Carrión et al., Neuroimmunomodulation 2013 : TNFa, LPS and poly ( I:C ) increased IL-12 and IL-23 levels in cells derived from patients presenting both pathologies ... VIP decreased IL-12p35 mRNA upregulation by poly ( I:C ) and IL-23p19 transcripts in LPS treated FLS
Aldhamen et al., PloS one 2013 : Furthermore, during pathogen recognition, ERAP1 regulates IL12 production by CD11c ( + ) DCs specifically, with increases in IL12 production positively correlated with an increased phagocytic activity of splenic DCs and macrophages ... Furthermore, during pathogen recognition, ERAP1 regulates IL12 production by CD11c ( + ) DCs specifically, with increases in IL12 production positively correlated with an increased phagocytic activity of splenic DCs and macrophages
Sabado et al., Journal of visualized experiments : JoVE 2013 : Our previous study established that Poly-ICLC is the most potent individual maturation stimulus for human DCs as assessed by an upregulation of CD83 and CD86, induction of interleukin-12 (IL-12) , tumor necrosis factor (TNF), interferon gamma induced protein 10 (IP-10), interleukmin 1 (IL-1), and type I interferons ( IFN ), and minimal interleukin 10 (IL-10) production
Römmler et al., J Immunol 2013 : Whereas TLR9 induced TNF-a secretion of bone marrow derived macrophages and conventional dendritic cells was equally inhibited by INH-ODN 2088 and G-modified INH-ODNs such as INH-ODN 24888, TLR7 induced TNF-a release and TLR7- and TLR9 induced IL-12p40 release were significantly more impaired by G-modified INH-ODNs
Ma et al., PloS one 2013 : Current models imply that Tim-3 expression on M/MO can deliver negative signaling to TLR mediated IL-12 expression through trans association with its ligand Galectin-9 (Gal-9) presented by other cells ... In this study, our data suggest that Tim-3 and IL-12/IL-23 gene transcriptions are regulated by enhanced or silenced Gal-9 expression within monocytes through synergizing with TLR signaling ... In this study, our data suggest that Tim-3 and IL-12/IL-23 gene transcriptions are regulated by enhanced or silenced Gal-9 expression within monocytes through synergizing with TLR signaling
Kubin et al., J Exp Med 1994 : The proliferative effect of anti-CD28 and IL-12 is resistant to moderate doses of cyclosporin A and is largely independent of endogenous IL-2, IL-12, in synergy with anti-CD28 or B7-transfected cells, is most effective in inducing interferon gamma (IFN-gamma) production, but production of tumor necrosis factor alpha and granulocyte/macrophage colony stimulating factor is also observed ... IL-12 induced IFN-gamma production in peripheral blood mononuclear cells is inhibited by the chimeric molecule CTLA-4 immunoglobulin, which prevents binding of CD28 to B7, suggesting that endogenous B7 on the mononuclear cells and IL-12 cooperate in inducing IFN-gamma production ... IL-10 inhibits both IL-12 production and B7 expression on monocytes
Ma et al., Infect Immun 1994 : As tumor necrosis factor alpha, IFN-beta, and IL-12 are potent activators of IFN-gamma production by T and NK cells, their presence in these cocultures could be responsible for the IFN-gamma production
Bacon et al., J Exp Med 1995 : Interleukin 12 (IL-12) induces tyrosine phosphorylation of JAK2 and TYK2 : differential use of Janus family tyrosine kinases by IL-2 and IL-12 ... Interleukin 12 (IL-12) induces tyrosine phosphorylation of JAK2 and TYK2 : differential use of Janus family tyrosine kinases by IL-2 and IL-12 ... IL-12 , but not IL-2, stimulates the tyrosine phosphorylation of TYK2 and JAK2, whereas JAK1 and JAK3, which are phosphorylated in response to IL-2, are not phosphorylated after IL-12 treatment ... IL-12 , but not IL-2, stimulates the tyrosine phosphorylation of TYK2 and JAK2 , whereas JAK1 and JAK3, which are phosphorylated in response to IL-2, are not phosphorylated after IL-12 treatment
Kricek et al., Eur J Immunol 1995 : After GAMD injection, two groups of induction profiles were observed in spleen, mesenteric lymph nodes and Peyer 's patches : while IL-4 and IL-12p35 gene transcription was strongly enhanced, IFN-gamma, IL-12p40 and IL-13 mRNA were only moderately induced ... After GAMD injection, two groups of induction profiles were observed in spleen, mesenteric lymph nodes and Peyer 's patches : while IL-4 and IL-12p35 gene transcription was strongly enhanced, IFN-gamma , IL-12p40 and IL-13 mRNA were only moderately induced ... After GAMD injection, two groups of induction profiles were observed in spleen, mesenteric lymph nodes and Peyer 's patches : while IL-4 and IL-12p35 gene transcription was strongly enhanced, IFN-gamma, IL-12p40 and IL-13 mRNA were only moderately induced ... After GAMD injection, two groups of induction profiles were observed in spleen, mesenteric lymph nodes and Peyer 's patches : while IL-4 and IL-12p35 gene transcription was strongly enhanced, IFN-gamma, IL-12p40 and IL-13 mRNA were only moderately induced
Skeen et al., J Immunol 1995 (Listeriosis) : This cytokine synergy pattern differs from that reported for NK cells, in which IL-12 in combination with either IL-2 or TNF-alpha induces NK cells to produce IFN-gamma
Tripp et al., J Immunol 1995 (Listeriosis) : During a secondary immune response to Listeria monocytogenes ( LM ), the production of IFN-gamma was still required for resistance, but it was considerably less dependent on IL-12 production ... However, anti-CD3-epsilon Ab-stimulated IFN-gamma production remained independent of IL-12 production
O'Sullivan et al., Ann Surg 1995 (Burns...) : Serious injury induced diminished production of IL-1 2 and a shift to the Th-2 phenotype with increased production of IL-4 and IL-10 , cytokines known to inhibit Th-1 function ... Serious injury induced diminished production of IL-1 2 and a shift to the Th-2 phenotype with increased production of IL-4 and IL-10, cytokines known to inhibit Th-1 function
Armant et al., J Immunol 1995 : Next, we show that, in the presence of monocytes, sCD23 is a potent costimulator of IL-2 or IL-12 induced IFN-gamma production by resting T cells in the absence of exogenous Ag and that this effect is partially reduced by anti-TNF-alpha mAb
Sandborg et al., J Immunol 1995 : IL-12 had an inhibitory effect on PBMC IL-4 production as previously described, but neither IL-6 nor TNF-alpha inhibited IL-4 production ... IL-12 did not inhibit IL-4 production in stimulated purified T cells
Prechel et al., Cancer Lett 1995 (Carcinoma, Lewis Lung...) : IL-12 slightly increased splenic IFN-gamma secretion, and increased cytotoxicity of lymph node ( but not spleen ) cells toward autologous tumor
Marshall et al., J Immunol 1995 (Hypersensitivity...) : IL-12 inhibits the production of IL-4 and IL-10 in allergen-specific human CD4+ T lymphocytes ... IL-12 inhibits the production of IL-4 and IL-10 in allergen-specific human CD4+ T lymphocytes ... The inhibitory effect of IL-12 on IL-4 synthesis was not dependent on the presence of IFN-gamma, was greatest when IL-12 was added at the initiation of culture, and was minimal when added late, indicating that resting memory CD4+ T cells were more sensitive than activated CD4+ T cells to the effects of IL-12 ... The effect of IL-12 on IL-4 and IL-10 synthesis was not dependent on the APC type, because IL-12 decreased IL-4 synthesis when either B cells or monocytes served as APCs ... The effect of IL-12 on IL-4 and IL-10 synthesis was not dependent on the APC type, because IL-12 decreased IL-4 synthesis when either B cells or monocytes served as APCs
Hayes et al., Blood 1995 : In this study, we examined the regulation of IL-12 expression by human monocytes in response to bacterial lipopolysaccharide (LPS) ... These results indicated that both p40 and p35 are LPS-inducible in monocytes following IFN-gamma pretreatment, and that the regulated expression of p35 controls the level of active IL-12 protein in purified human monocytes ... These results indicated that both p40 and p35 are LPS-inducible in monocytes following IFN-gamma pretreatment, and that the regulated expression of p35 controls the level of active IL-12 protein in purified human monocytes ... These results indicated that both p40 and p35 are LPS-inducible in monocytes following IFN-gamma pretreatment, and that the regulated expression of p35 controls the level of active IL-12 protein in purified human monocytes
Liu et al., Cell Immunol 1995 (Listeriosis) : Although we could detect no obvious lag between the time of onset of IL-12 and IFN-gamma accumulation in vivo, infusions of anti-IL-12 antibodies markedly reduced IFN-gamma expression implying that IL-12 production precedes and directs IFN-gamma production
Bradley et al., J Immunol 1995 : Our results suggest that memory and naive CD4 cells undergo parallel development following Ag stimulation, initially secreting predominantly IL-2 and differentiating in response to IL-4, IFN-gamma, and IL-12 into polarized effector subsets
Ye et al., J Leukoc Biol 1995 : Cellular and molecular mechanisms of IFN-gamma production induced by IL-2 and IL-12 in a human NK cell line ... Although it has been reported that there are some T cell lines that produce IFN-gamma in response to IL-2 and IL-12 stimulation, there has as yet been no report of a natural killer (NK) cell line that responds in a similar manner ... Furthermore, both transforming growth factor-beta and genistein, a tyrosine kinase inhibitor, could suppress IL-2 and IL-12 signaling but CsA was generally inactive
Brunda et al., J Immunother Emphasis Tumor Immunol 1995 (Neoplasms, Experimental) : Since one of the properties of IL-12 is its ability to stimulate production of IFN-gamma, the role of IFN-gamma in mediating the antitumor activity of IL-12 was evaluated ... In addition, the antitumor effect of IL-12 was reduced in nude mice compared with euthymic mice, but an approximately 10-fold higher level of serum IFN-gamma was induced in nude than in euthymic mice
Schijns et al., J Immunol 1995 : In wild-type mice administration of recombinant IFN-gamma but not IL-2 mimicked the immune stimulating activity of IL-12 ; it is therefore likely that the IL-12 adjuvant activity is largely mediated by physiologic IFN-gamma
Paganin et al., J Clin Invest 1995 (HIV Infections) : IL-12 enhances the patient 's depressed natural killer cell cytotoxic activity, peripheral blood lymphocyte production of interferon-gamma (IFN-gamma) , and proliferative T cell response in vitro to recall antigens, HIV antigens, alloantigens, and mitogens
Jeannin et al., Eur J Immunol 1995 : We found that IL-12 (i) enhances , as previously reported, IFN-gamma production by Th0 TCC and, to a smaller extent, by Th2-like TCC, ( ii ) increases the proliferation of Th0 and Th2-like TCC and ( iii ) unexpectedly, synergizes with T cell receptor associated or nonspecific stimuli in increasing IL-4 production by these TCC
Grewe et al., J Invest Dermatol 1995 (Dermatitis, Atopic) : IFN-gamma production by T cells may be induced by the cytokine IL-12
Salkowski et al., J Immunol 1995 : In the spleen, IL-6 and TNF-alpha were induced by 30 min after LPS challenge, while increases in IL-10 and IL-12 ( p40 ) were delayed in onset ... In the spleen, IL-6 and TNF-alpha were induced by 30 min after LPS challenge, while increases in IL-10 and IL-12 ( p40 ) were delayed in onset ... The results from Cl2MBP-liposome treated mice also suggested that splenic macrophages were the primary producers of LPS induced IL-1 beta, IL-6, IL-12 ( p40 ), and IL-1 receptor antagonist (IL-1ra) mRNA, but not IL-10 and TNF-alpha mRNA
Ploemacher et al., Leukemia 1993 : IL-12 did not support colony formation as a single factor, nor in the presence of IL-11 or SF ... In IL-3 + SF-containing cultures, IL-12 further increased colony numbers and a higher percentage of colonies expressed neutrophilic, megakaryocytic, erythroid, monocytic, blast cell, and/or mast cell lineages
Ploemacher et al., Leukemia 1993 : Previous data have shown that IL-12 may act as a potent synergistic factor stimulating multilineage expression of hemopoietic stem cells in the presence of IL-3 in combination with Steel factor ( SF ) or IL-11
Skeiky et al., J Exp Med 1995 (Leishmaniasis, Cutaneous...) : The production of IFN-gamma by patient PBMC stimulated with either rLeIF or parasite lysate was IL-12 dependent , whereas anti-IFN-gamma monoclonal antibody only partially blocked the LeIF induced production of IL-12
Wysocka et al., Eur J Immunol 1995 (Shock, Septic) : The priming effect of BCG on IFN-gamma production appears to be mostly due to its ability to increase TNF-alpha production, which acts as cofactor with LPS induced IL-12 in inducing IFN-gamma production, as shown by the ability of injection of TNF-alpha and LPS ( 1 microgram/mouse ), but not LPS alone, to induce IFN-gamma production ... Thus, IL-12 is required for IFN-gamma production and lethality in an endotoxic shock model in mice
Mocci et al., J Immunol 1995 (Leishmaniasis, Cutaneous) : In contrast, the presence of IFN-gamma and IL-12 during the Th2 cell stimulation enhanced IFN-gamma production but was not sufficient to induce a complete conversion of a Th2 into a Th1-like population
Scharton-Kersten et al., J Leukoc Biol 1995 (Leishmaniasis, Cutaneous) : Together, these observations support a paradigm of in vivo Th1 cell development that involves IL-12 dependent stimulation of IFN-gamma production by NK cells
Jacobson et al., J Exp Med 1995 : IL-12 selectively induced nuclear DNA binding complexes that contained Stat3 and Stat4, recently cloned members of the family of signal transducers and activators of transcription ( STATs ) ... IL-12 selectively induced nuclear DNA binding complexes that contained Stat3 and Stat4 , recently cloned members of the family of signal transducers and activators of transcription ( STATs )
Eng et al., J Exp Med 1995 : The stimulatory effects of interleukin (IL)-12 on hematopoiesis are antagonized by IL-12 induced interferon gamma in vivo ... Here, we used interferon (IFN) gamma receptor-deficient ( IFN gamma R-/- ) mice to investigate whether the in vivo inhibition of hematopoiesis by IL-12 is indirectly mediated by IL-12 induced IFN-gamma ... Thus, alterations mediated by IL-12 induced IFN-gamma include reduction in bone marrow cellularity and hematopoietic progenitors, as well as pronounced splenomegaly, largely caused by NK cell infiltration
Cesano et al., Cancer Immunol Immunother 1995 : CsA did not impair the ability of TALL-104 and LAK cells to produce interferon (IFN) gamma, tumor necrosis factor (TNF) alpha, and granulocyte/macrophage-colony-stimulatory factor ( GM-CSF ) in response to IL-2, IL-12 , or tumor targets ... CsA did not impair the ability of TALL-104 and LAK cells to produce interferon (IFN) gamma, tumor necrosis factor (TNF) alpha, and granulocyte/macrophage-colony-stimulatory factor ( GM-CSF ) in response to IL-2, IL-12 , or tumor targets ... CsA did not impair the ability of TALL-104 and LAK cells to produce interferon (IFN) gamma , tumor necrosis factor (TNF) alpha, and granulocyte/macrophage-colony-stimulatory factor ( GM-CSF ) in response to IL-2, IL-12 , or tumor targets
Warren et al., J Immunol 1995 : Production of IL-5 by human NK cells and regulation of IL-5 secretion by IL-4, IL-10, and IL-12 ... IL-5 secretion, particularly in primary cultures, is augmented by IL-4 and is inhibited by IL-12 and IL-10
Hunter et al., Eur J Immunol 1995 (Toxoplasmosis, Animal) : Stimulation of splenocytes from SCID mice with IL-12 in combination with either TNF-alpha or IL-1 beta resulted in production of high levels of IFN-gamma
Haku et al., Jpn J Cancer Res 1995 : Induction by IL-12 of IFN gamma production and cytotoxic activity of CD8+ cells was also augmented by co-cultivation with monocytes or AM
Hegde et al., Cell Immunol 1995 (Neoplasms, Experimental) : AK-5 tumor induced expression of interleukin-12 : role of IL-12 in NK-mediated AK-5 regression
Cooper et al., Immunology 1995 (Tuberculosis) : IL-12 also increased the production of IFN-gamma by both splenocytes derived from infected animals treated in vivo and by antigen stimulated CD4 cells from untreated infected animals, with maximal effects at times associated with the expansion of antigen-specific CD4 T cells in vivo
Lester et al., J Immunol 1995 (Dermatitis, Atopic) : Because AD is associated with increased production of IL-4 and IL-10, we examined the effect of IL-4 on IL-12- or TSST-1 induced IFN-gamma production in normal donors ... IL-4 inhibited IL-12 induced IFN-gamma production ... Our data suggest that despite normal levels of IL-12 production and IL-12R expression, PBMC from AD patients are unable to generate normal IL-12 induced IFN-gamma responses
Santambrogio et al., J Neuroimmunol 1995 (Encephalomyelitis, Autoimmune, Experimental) : IL-10 ( 1 microgram/day ) was not protective under these conditions and IL-12 ( 50 ng/day ) significantly increased the severity and mortality of EAE when given on days 0-4 after MSCH + CFA
Quelle et al., Mol Cell Biol 1995 : In contrast, IL-2, IL-3, and erythropoietin induce the tyrosine phosphorylation of Stat5 while IL-12 uniquely induces the tyrosine phosphorylation of Stat4 ... In contrast, IL-2, IL-3, and erythropoietin induce the tyrosine phosphorylation of Stat5 while IL-12 uniquely induces the tyrosine phosphorylation of Stat4 ... In contrast, IL-2, IL-3, and erythropoietin induce the tyrosine phosphorylation of Stat5 while IL-12 uniquely induces the tyrosine phosphorylation of Stat4 ... In contrast, IL-2 , IL-3, and erythropoietin induce the tyrosine phosphorylation of Stat5 while IL-12 uniquely induces the tyrosine phosphorylation of Stat4 ... In contrast, IL-2, IL-3 , and erythropoietin induce the tyrosine phosphorylation of Stat5 while IL-12 uniquely induces the tyrosine phosphorylation of Stat4
Ogata et al., Br J Haematol 1995 (Myelodysplastic Syndromes) : The effects of interleukin 12 (IL-12) on natural killer ( NK ) cell cytotoxicity and on the production of interferon-gamma (IFN-gamma) and tumour necrosis factor-alpha (TNF-alpha) were examined in 15 patients with myelodysplastic syndromes (MDS), which are well known to have immunologic defects, and in 11 normal subjects ... The effects of interleukin 12 (IL-12) on natural killer ( NK ) cell cytotoxicity and on the production of interferon-gamma (IFN-gamma) and tumour necrosis factor-alpha (TNF-alpha) were examined in 15 patients with myelodysplastic syndromes (MDS), which are well known to have immunologic defects, and in 11 normal subjects
Chouaib et al., Proc Natl Acad Sci U S A 1994 : IL-12 increased granzyme B and perforin mRNA accumulation in major histocompatibility complex class I-primed lymphocytes, suggesting that this cytokine activates these two genes in CTL precursors
Wang et al., Proc Natl Acad Sci U S A 1994 (Leishmaniasis, Cutaneous) : Given with the inoculation of parasites, IL-12 induced IFN-gamma and IL-10 and markedly suppressed IL-4 ... Given with the inoculation of parasites, IL-12 induced IFN-gamma and IL-10 and markedly suppressed IL-4 ... Given with the inoculation of parasites, IL-12 induced IFN-gamma and IL-10 and markedly suppressed IL-4 ... Induction of IFN-gamma and IL-10 mRNA by IL-12 also occurred in infected SCID mice ... Given after day 14 of infection, however, IL-12 not only induced IFN-gamma and IL-10 but also induced IL-4 in normal and IFN-gamma 0/0 mice ... Given after day 14 of infection, however, IL-12 not only induced IFN-gamma and IL-10 but also induced IL-4 in normal and IFN-gamma 0/0 mice ... Given after day 14 of infection, however, IL-12 not only induced IFN-gamma and IL-10 but also induced IL-4 in normal and IFN-gamma 0/0 mice
König et al., Int Arch Allergy Immunol 1995 (Dermatitis, Atopic) : In contrast to normal donors T cells from donors with AD predominantly express mRNA for IL-4, IL-5, and only diminished levels for IFN-gamma and IL-12 upon stimulation with SEB and SEA ... In contrast to normal donors T cells from donors with AD predominantly express mRNA for IL-4, IL-5, and only diminished levels for IFN-gamma and IL-12 upon stimulation with SEB and SEA
Rook et al., J Immunol 1995 (Sezary Syndrome...) : Because IL-12 is a potent inducer of IFN-gamma production, and causes the activation of cytotoxic lymphocytes, we examined the production of IL-12 by PBMC from SzS patients and whether IL-12 could alter the unfavorable cytokine balance typical of SzS and thus lead to correction of immune defects
D'Andrea et al., J Exp Med 1995 : Addition of IL-4 and IL-13 to peripheral blood mononuclear cell ( PBMC ) cultures inhibited production of IL-12 , tumor necrosis factor (TNF)-alpha, IL-10, and IL-1 beta induced by lipopolysaccharide (LPS) or Staphylococcus aureus added simultaneously with the cytokines ... However, pretreatment of PBMC with IL-4 or IL-13 for > or = 20 h enhanced the production of IL-12 and TNF-alpha in response to LPS or S. aureus several fold in these cells ; this IL-4 induced priming for the two cytokines was inhibited by anti-IL-4 neutralizing antibodies ... IL-4 priming also enhanced the accumulation of IL-12 and TNF-alpha mRNA induced by LPS and S. aureus
Leung et al., J Exp Med 1995 : More importantly, induction of toxin induced CLA expression was blocked by anti-IL-12 , and the addition of IL-12 to PHA stimulated T cells induced CLA, but not alpha e beta 7-integrin , expression
van der Pouw Kraan et al., J Exp Med 1995 : Recombinant human IL-10 also inhibited IL-12 and IL-6 production
Cassatella et al., Eur J Immunol 1995 : IL-10 , an inhibitor of the production of various cytokines in PMN, also suppressed IL-12 mRNA accumulation and secretion by PMN
Gillessen et al., Eur J Immunol 1995 : The ( p40 ) 2 was 25- to 50-fold more active than the p40 monomer in causing specific, dose dependent inhibition of IL-12 induced mouse concanavalin A ( Con A ) blast proliferation and could also inhibit IL-12 induced interferon-gamma (IFN-gamma) secretion by mouse splenocytes and IL-12 dependent activation of mouse NK cells
Orange et al., J Exp Med 1995 (Lymphocytic Choriomeningitis) : Northern blot and in situ hybridization analyses demonstrated that IL-12 induced TNF-alpha expression and that LCMV infection synergized with IL-12 for induction of this factor
Rabinowich et al., Cell Immunol 1993 (Neoplasms) : Among the beta 2 integrins, IL12 selectively increased expression of CD11a on NK cells, although to a significantly lower level than that induced by IL2
Morris et al., J Immunol 1994 : In untreated mice IL-12 enhanced IFN-gamma and IL-10 gene expression and protein secretion, reduced base line IL-3 and IL-4 gene expression, and increased serum IgG2a concentration ... In untreated mice IL-12 enhanced IFN-gamma and IL-10 gene expression and protein secretion, reduced base line IL-3 and IL-4 gene expression, and increased serum IgG2a concentration ... In untreated mice IL-12 enhanced IFN-gamma and IL-10 gene expression and protein secretion, reduced base line IL-3 and IL-4 gene expression, and increased serum IgG2a concentration ... In untreated mice IL-12 enhanced IFN-gamma and IL-10 gene expression and protein secretion, reduced base line IL-3 and IL-4 gene expression, and increased serum IgG2a concentration ... These observations demonstrate that : 1 ) IL-12 may limit its own effects by inducing the production of a cytokine ( IL-10 ) that down-regulates both IL-12 production and IL-12 induced IFN-gamma production ; 2 ) IL-12 inhibits the production of at least one cytokine, IL-3, that is not generally regarded to be strictly Th1- or Th2 associated ; 3 ) IL-12 inhibits switching to IgE secretion to a greater extent than it inhibits switching to other Ig isotypes ; and 4 ) the in vivo effects of IL-12 are, to a large extent, IFN-gamma dependent
Yoshida et al., Biochem Biophys Res Commun 1994 : IFN-gamma induces IL-12 mRNA expression by a murine macrophage cell line, J774 ... The IFN-gamma-inducible IL-12 mRNA was blocked by two protein tyrosine kinase ( PTK ) inhibitors, genistein and herbimycin A, suggesting that PTK is involved in the IFN-gamma-inducible IL-12 mRNA expression
Tripp et al., J Immunol 1994 (Listeriosis) : Interleukin-12 (IL-12) is necessary for the production of IFN-gamma by NK cells during the generation of innate immunity and by T cells for the development of the Th1 response during specific cell mediated immunity ... Thus, IL-12 is critical for resistance to infection with Listeria monocytogenes, and this resistance is mediated through stimulation by IL-12 of IFN-gamma production
Jewett et al., Cell Immunol 1994 : The 55-kDa TNF receptor ( TNF-R p55 ), however, was not affected by IL-12 , but in the presence of anti-IFN-gamma antibody, its expression was enhanced
Chehimi et al., J Exp Med 1994 (HIV Infections) : The defect in IL-12 production is not due to hyperproduction of IL-10 , a cytokine exerting an autocrine negative feedback on IL-12 production, but was directly related to HIV infection, as suggested by the reduced ability of monocytes infected in vitro with HIV to produce IL-12
Gately et al., Int Immunol 1994 : Liver and splenic lymphoid cells from IL-12 treated mice, unlike liver and splenic lymphoid cells from control mice, spontaneously secreted IFN-gamma in vitro, suggesting that they had been induced by IL-12 to produce IFN-gamma in vivo ... Thus, these studies demonstrate that IL-12 can enhance NK and CTL activity and induce IFN-gamma production in vivo, as well as in vitro, and suggest possible mechanisms by which IL-12 may exert therapeutic effects in the treatment of some tumors and infectious diseases
Romani et al., Eur J Immunol 1994 (Candidiasis) : Overall, our results showed that ( i ) antigen-specific secretion of IFN-gamma protein in vitro by CD4+ cells occurred only in healing infection ; ( ii ) IL-4- and IL-10 producing CD4+ cells would expand in nonhealer mice in the face of high levels of circulating IFN-gamma, likely released by CD4- CD8- lymphocytes ; ( iii ) a finely regulated IFN-gamma production correlated in the healer mice with IL-12 mRNA detection, and IL-12 was required in vitro for yeast induced development of IFN-gamma producing CD4+ cells
Bloom et al., J Immunol 1994 : The augmentation of CTL by IL-12 was not associated with increased incorporation of [ 3H ] TdR. Increased IFN-gamma production was not required for IL-12 to augment CTL activity because when three different Abs against mouse IFN-gamma were each included in the MLC at concentrations abolishing recoverable IFN-gamma protein, they did not inhibit the IL-12 effect but, under some conditions, further increased CTL activity
Finkelman et al., J Exp Med 1994 (Intestinal Diseases, Parasitic...) : The cytokine interleukin (IL) 12 stimulates T cell and natural killer cell production of interferon (IFN) gamma and inhibits T cell production of IL-4 ... IL-12 stimulated IFN-gamma and IL-10 gene expression during primary and secondary N. brasiliensis infections and inhibited IL-3 , IL-4, IL-5, and IL-9 gene expression during primary infections but had little inhibitory effect during secondary infections ... IL-12 stimulated IFN-gamma and IL-10 gene expression during primary and secondary N. brasiliensis infections and inhibited IL-3, IL-4 , IL-5, and IL-9 gene expression during primary infections but had little inhibitory effect during secondary infections ... IL-12 stimulated IFN-gamma and IL-10 gene expression during primary and secondary N. brasiliensis infections and inhibited IL-3, IL-4, IL-5 , and IL-9 gene expression during primary infections but had little inhibitory effect during secondary infections ... IL-12 stimulated IFN-gamma and IL-10 gene expression during primary and secondary N. brasiliensis infections and inhibited IL-3, IL-4, IL-5, and IL-9 gene expression during primary infections but had little inhibitory effect during secondary infections ... IL-12 stimulated IFN-gamma and IL-10 gene expression during primary and secondary N. brasiliensis infections and inhibited IL-3, IL-4, IL-5, and IL-9 gene expression during primary infections but had little inhibitory effect during secondary infections ... IL-12 stimulated IFN-gamma and IL-10 gene expression during primary and secondary N. brasiliensis infections and inhibited IL-3, IL-4, IL-5, and IL-9 gene expression during primary infections but had little inhibitory effect during secondary infections
Yanagida et al., J Immunol 1994 : In these experiments, effects of IL-12 on the proliferation and IL-2R expression of Th1 and Th2 clones were studied ... Effects of IL-12 on these T cell functions were indicated to be exerted in concert with IL-2, although IL-12 did not enhance IL-2 production of Th1 clones ... IL-12 also induced proliferation and IL-2R alpha-chain expression of Th1 clone stimulated with anti-CD3 in the absence of APC, indicating that IL-12 exerted the effect on Th1 cells directly and other costimulator signal from APC is not required for the function of IL-12 ... These results suggest that IL-12 plays an important role in IL-2R alpha-chain expression and proliferation of Th1 clones, but not Th2 clones, as a second signal
Hunter et al., Infect Immun 1994 (Toxoplasmosis, Animal) : Further analysis revealed that interleukin 10 (IL-10) inhibited, whereas tumor necrosis factor alpha (TNF-alpha) and IL-12 enhanced , IFN-gamma production by spleen cells from infected or uninfected mice
Aste-Amezaga et al., Cell Immunol 1994 : It was found that IL-12 induces mRNA accumulation and production of GM-CSF and TNF-alpha from both T and NK cells and, as tested on NK cells only, induces M-CSF mRNA accumulation ... It was found that IL-12 induces mRNA accumulation and production of GM-CSF and TNF-alpha from both T and NK cells and, as tested on NK cells only, induces M-CSF mRNA accumulation ... It was found that IL-12 induces mRNA accumulation and production of GM-CSF and TNF-alpha from both T and NK cells and, as tested on NK cells only, induces M-CSF mRNA accumulation ... IL-12 induction of perforin mRNA accumulation was not synergistic with either IL-2 or anti-CD16 stimulation, whereas granzyme B mRNA accumulation, induced by IL-2 or anti-CD16 stimulation, was slightly potentiated by IL-12
Nastala et al., J Immunol 1994 (Adenocarcinoma...) : These results indicate that IL-12 is an effective and minimally toxic antitumor agent in murine tumor models and leads to an immune mediated rejection involving, at least in part, IFN-gamma, CD4+ , and CD8+ cells ... These results indicate that IL-12 is an effective and minimally toxic antitumor agent in murine tumor models and leads to an immune mediated rejection involving, at least in part, IFN-gamma , CD4+, and CD8+ cells
Oswald et al., J Immunol 1994 : Anti-IFN-gamma also inhibited the enhancement of IFN-gamma responses induced by IL-12 but only if the mAb was administered systemically plus locally
Ozmen et al., J Exp Med 1994 (Shwartzman Phenomenon) : These results strongly suggest that LPS induces the release of IL-12, that IL-12 induces the production of IFN-gamma , and that IFN-gamma is the cytokine that primes macrophages and other cell types ... These results strongly suggest that LPS induces the release of IL-12 , that IL-12 induces the production of IFN-gamma, and that IFN-gamma is the cytokine that primes macrophages and other cell types
Heinzel et al., Infect Immun 1994 : Because interleukin 12 (IL-12) is a known inducer of IFN-gamma synthesis by cultured T cells and NK cells, we examined whether IL-12 might be involved in IFN-gamma release during endotoxemia
Bertagnolli et al., Transplantation 1994 : Freshly isolated CD3+ cells did not proliferate in response to IL-12 , nor did culture of these cells in IL-12 lead to upregulation of IL-2 receptor
Nagy et al., Cell Immunol 1994 : We investigated the role of IL-12 in regulating IL-2 and IFN-gamma production in primary culture of human T cells ... We investigated the role of IL-12 in regulating IL-2 and IFN-gamma production in primary culture of human T cells
Alzona et al., J Immunol 1995 : IL-12 activates IFN-gamma production through the preferential activation of CD30+ T cells ... Inasmuch as the production of IFN-gamma is regulated by IL-2 and IL-12 , we have examined the effects of these cytokines on the proliferation, induction, and function of CD30+ and CD30- T cell subsets ... Thus, CD30+ T cells are preferentially regulated by IL-12 , and the effects of IL-12 on T cell IFN-gamma production are mediated largely through its effects on the CD30+ subset
Tripp et al., Proc Natl Acad Sci U S A 1993 (Listeriosis) : We demonstrate that IFN-gamma production from SCID splenocytes is stimulated by interleukin (IL) 12, tumor necrosis factor alpha (TNF-alpha), and IL-2 but is inhibited by IL-10, IL-10, IL-12 , and TNF are induced by heat killed Listeria monocytogenes ( hk-LM ) from SCID splenocytes and peritoneal macrophages ... IL-12 production is necessary for hk-LM to stimulate IFN-gamma production by SCID splenocytes since neutralization of IL-12 totally blocks IFN-gamma production in this system ... In contrast, IL-10 inhibits hk-LM induced IFN-gamma production at two levels : ( i ) by inhibiting TNF and IL-12 production from these cultures ( presumably from the macrophage ) and ( ii ) by inhibiting the stimulatory effects of IL-12 and TNF-alpha on NK-cell IFN-gamma production
Naume et al., Cytokine 1993 : Furthermore, IL-4 induced a slight increase in the IL-12 stimulated TNF production ... IL-12 enhanced the IL-12 receptor (R) expression and IL-4R expression in the CD56+ NK cells ... IL-12 enhanced the IL-12 receptor (R) expression and IL-4R expression in the CD56+ NK cells ... The increased IL-4R expression induced by IL-12 and the increased IL-12R expression induced by IL-4 may explain the synergistic proliferative activity detected in response to IL-12 and IL-4 ... The increased IL-4R expression induced by IL-12 and the increased IL-12R expression induced by IL-4 may explain the synergistic proliferative activity detected in response to IL-12 and IL-4
Germann et al., Eur J Immunol 1993 : Synthesis of interferon (IFN)-gamma by Th1 cells can be triggered by IL-2 plus IL-12 ... In contrast to the IFN-gamma production observed after T cell receptor (TcR) CD3 stimulation of Th1 cells with lectin Concanavalin A the IFN-gamma production induced by IL-12 + IL-2 is insensitive to the immunosuppressive drug cyclosporin A ... Furthermore, IL-12 enhances the TcR/CD3 induced synthesis of IFN-gamma of several Th1 clones
Wu et al., J Immunol 1993 : IL-12 induces the production of IFN-gamma by neonatal human CD4 T cells ... IL-12 also induces neonatal CD4 T cells to produce lymphotoxin but not IL-2, TNF-alpha , or IL-4 ... IL-12 also induces neonatal CD4 T cells to produce lymphotoxin but not IL-2 , TNF-alpha, or IL-4 ... IL-12 also induces neonatal CD4 T cells to produce lymphotoxin but not IL-2, TNF-alpha, or IL-4
D'Andrea et al., J Exp Med 1993 : Although IL-10 is able to block the production of NKSF/IL-12, a powerful inducer of IFN-gamma both in vitro and in vivo, the mechanism of inhibition of IFN-gamma by IL-10 can not be explained only on the basis of inhibition of NKSF/IL-12 because IL-10 can partially inhibit IFN-gamma production induced by NKSF/IL-12 , and also, the IFN-gamma production in response to various stimuli in the presence of neutralizing antibodies to NKSF/IL-12
Mehrotra et al., J Immunol 1993 : IL-12 augmentation of proliferation and cytotoxicity of CD8+ T cells was not inhibited by a mAb to the p55 subunit of the IL-2 receptor ( alpha-Tac ) at a concentration sufficient to block the activity of exogenously added IL-2, indicating that the activity of IL-12 did not require IL-2
Mattner et al., Eur J Immunol 1993 : Furthermore, the synthesis of interferon (IFN)-gamma by mouse splenocytes activated with IL-2 and IL-12 was suppressed by IL-12p40 ... IFN-gamma production by Th1 cells induced in a T cell receptor independent way by macrophages and IL-2 or macrophages and IL-12 was greatly reduced by IL-12p40 providing evidence for the endogenous synthesis of IL-12 in the Th1 cell, macrophage and IL-2 co-cultures ... Only IL-2 + IL-12 but not IL-2 + tumor necrosis factor induced aggregation was inhibited in a dose dependent manner by IL-12p40 ... Only IL-2 + IL-12 but not IL-2 + tumor necrosis factor induced aggregation was inhibited in a dose dependent manner by IL-12p40
Halpern et al., Cell Immunol 1996 : Interleukin-12 (IL-12) was detectable in supernatants from DNA stimulated splenocytes before IFN-gamma, and neutralizing antibodies directed against IL-12 markedly inhibited the induction of IFN-gamma ... Taken together, these results indicate that the stimulation of IFN-gamma production by bacterial DNA is mediated by IL-12 and TNF-alpha and point to macrophages/monocytes as targets of action of this macromolecule
Ma et al., J Exp Med 1996 : IFN-gamma enhances the ability of the phagocytic cells to produce IL-12 and other proinflammatory cytokines ... Thus, IL-12 induced IFN-gamma acts in a positive feedback loop that represents an important amplifying mechanism in the inflammatory response to infections ... We show here that IFN-gamma enhances IL-12 production mostly by priming phagocytic cells for lipopolysaccharide (LPS) induced transcription of the IL-12 p40 gene, which encodes the heavy chain of the IL-12 heterodimer ; furthermore, IFN-gamma directly induces transcription of the IL-12 p35 gene, which encodes the light chain of IL-12, and has at least an additive effect with LPS stimulation in inducing its transcription ... We show here that IFN-gamma enhances IL-12 production mostly by priming phagocytic cells for lipopolysaccharide (LPS) induced transcription of the IL-12 p40 gene, which encodes the heavy chain of the IL-12 heterodimer ; furthermore, IFN-gamma directly induces transcription of the IL-12 p35 gene, which encodes the light chain of IL-12, and has at least an additive effect with LPS stimulation in inducing its transcription ... Because the RAW264.7 cell line is easily transfectable and regulates the endogenous IL-12 p40 gene in response to IFN-gamma or LPS similarly to human monocytes, it was used for analysis of the regulation of the cloned human IL-12 p40 promoter ... Because the RAW264.7 cell line is easily transfectable and regulates the endogenous IL-12 p40 gene in response to IFN-gamma or LPS similarly to human monocytes, it was used for analysis of the regulation of the cloned human IL-12 p40 promoter
Fallarino et al., J Immunol 1996 (Mast-Cell Sarcoma) : Conversely, exogenous IL-12 delivered early during challenge with P1.HTR cells induced high IFN-gamma production and resulted in tumor rejection in most mice
Orange et al., J Immunol 1996 (Herpesviridae Infections...) : An absolute and restricted requirement for IL-12 in natural killer cell IFN-gamma production and antiviral defense ... The MCMV induced IL-12 was responsible for early NK cell IFN-gamma production and viral control, as in vivo IL-12 neutralization by Ab treatment blocked both of these
Snijders et al., J Immunol 1996 : IL-12 enhances IFN-gamma production by NK cells and T lymphocytes and plays a pivotal role in the development of Th1 cells
Bohn et al., J Immunol 1996 (Yersinia Infections) : Spleen cells from both strains of mice produced Yersinia triggered IFN-gamma in an IL-12 dependent manner, although those from BALB/c mice produced 10-fold lesser quantities ... In summary, our data suggest that IL-12 is rapidly induced by Y. enterocolitica infection and required for IFN-gamma production by NK cells as well by CD4+ T cells ... Although BALB/c and C57BL/6 mice produced comparable quantities of IL-12, IFN-gamma production, and thus resistance to yersiniae, can be increased by exogenous IL-12 only in BALB/c, not in C57BL/6, mice
Benjamin et al., J Immunol 1996 (Acquired Immunodeficiency Syndrome...) : The CL produced IL-10 , a known inhibitor of IL-12 , but anti-IL-10 Abs did not neutralize IL-12 ... Similarly, neutralizing anti-IFN gamma Abs or IFN gamma did not affect B cell IL-12 ... Our data suggest that : 1 ) AABCL constitutively secrete large amounts of IL-12, contrasting with low IL-12 production by HIV-1 infected PBMC ; 2 ) lack of IL-12 expression in EBV- AABCL suggests that in vivo exposure of B cells to HIV-1 only does not induce IL-12 secretion and that both HIV-1 and EBV are required ; 3 ) the autocrine negative effect of IL-10 on IL-12 in monocytes and the enhancing effect of IFN gamma on IL-12 secretion do not apply to B cells derived from AIDS patients ... Our data suggest that : 1 ) AABCL constitutively secrete large amounts of IL-12, contrasting with low IL-12 production by HIV-1 infected PBMC ; 2 ) lack of IL-12 expression in EBV- AABCL suggests that in vivo exposure of B cells to HIV-1 only does not induce IL-12 secretion and that both HIV-1 and EBV are required ; 3 ) the autocrine negative effect of IL-10 on IL-12 in monocytes and the enhancing effect of IFN gamma on IL-12 secretion do not apply to B cells derived from AIDS patients
Hilkens et al., J Immunol 1996 : IL- 12 enhances IFN-gamma production by activated CD4+ T cells, whereas PGE2 inhibits the secretion of this cytokine
Urban et al., J Immunol 1996 (Cryptosporidiosis...) : Because studies in mice have implicated IFN-gamma in protection against this parasite, and IL-12 can induce IFN-gamma production, we examined the ability of IL-12 to prevent or cure Cp infection in neonatal BALB/c and SCID mice
Jung et al., Eur J Immunol 1996 : Under these conditions, however, IL-12 inhibited IL-13 production by CD45RA+ T cells and to a lesser extent by CD45RO+ T cells in a dose dependent fashion ... These inhibiting effects were not related to enhanced IFN-gamma production induced by IL-12 , since IFN-gamma by itself did not affect IL-13 production
Hino et al., Eur J Immunol 1996 : In our experiments, p40 mRNA accumulation was shown to be affected by IL-10 more severely than the accumulation of p35 mRNA, indicating that IL-10 regulates IL-12 production by APC mainly by affecting p40 mRNA accumulation ... In our experiments, p40 mRNA accumulation was shown to be affected by IL-10 more severely than the accumulation of p35 mRNA, indicating that IL-10 regulates IL-12 production by APC mainly by affecting p40 mRNA accumulation
Heufler et al., Eur J Immunol 1996 : DC exhibited, however, features that had not been seen with other antigen presenting cells : they produced bioactive IL-12 upon antigen-specific interaction with T cells without any other stimuli ; in an allogeneic mixed leukocyte reaction model, neutralizing anti-IL-12 antibodies showed that DC-derived IL-12 was critical for optimal proliferation and IFN-gamma production by activated Th1 blasts ; and finally, the priming of resting, naive allogeneic T cells by DC, followed by restimulation of primed T blasts by DC, skewed the response to Th1 without the need for any exogenous cytokines or stimuli such as microorganisms
Chong et al., Infect Immun 1996 : By 4 h postexposure, viable organisms had induced a 27-fold increase in IL-12 p40 mRNA levels while killed organisms had induced a 9-fold increase in IL-12 p40 mRNA levels ... By 4 h postexposure, viable organisms had induced a 27-fold increase in IL-12 p40 mRNA levels while killed organisms had induced a 9-fold increase in IL-12 p40 mRNA levels
Meyaard et al., J Immunol 1996 : IL-12 induced IL-10 production by human T cells as a negative feedback for IL-12 induced immune responses
Sgadari et al., Blood 1996 : Interleukin 12 (IL-12) , a multifunctional cytokine produced by macrophages and B-cell lines, induces interferon-gamma (IFN-gamma) production, stimulates growth of both T and natural killer cells, promotes Th1-type helper T-cell responses, and inhibits neovascularization ... Because the human interferon-inducible protein 10 (IP-10) can also inhibit neovascularization, we tested whether IP-10 , induced by IL-12 through the intermediate IFN-gamma, might be a mediator of IL-12 angiogenesis inhibition ... Because the human interferon-inducible protein 10 (IP-10) can also inhibit neovascularization, we tested whether IP-10, induced by IL-12 through the intermediate IFN-gamma , might be a mediator of IL-12 angiogenesis inhibition ... Murine IL-12 induced murine IP-10 expression in mouse splenocytes, and human IFN-gamma induced human IP-10 expression in purified human endothelial cells, suggesting that IL-12 can induce IP-10 expression in certain cells ... Murine IL-12 induced murine IP-10 expression in mouse splenocytes, and human IFN-gamma induced human IP-10 expression in purified human endothelial cells, suggesting that IL-12 can induce IP-10 expression in certain cells
Trinchieri et al., J Leukoc Biol 1996 : This IFN-gamma potentiates antigen presenting cell functions important in clearing infections agents ( phagocytosis, oxidative burst, and production of nitrous oxide ) and also increases further production of IL-12
Igarashi et al., Eur J Immunol 1996 : In the present experiments, IL-12 induced enhancement of IL-2R alpha expression of Th1 clones stimulated with B cells as antigen presenting cells (APC) is suppressed by the addition of anti-B7-1
Wu et al., Eur J Immunol 1996 : We now report that a pair of monoclonal antibodies ( mAb ), 2B10 and 2.4E6, directed against different epitopes on the IL-12R beta chain, when used in combination, strongly inhibited IL-12 induced proliferation of activated T cells, IL-12 induced secretion of interferon-gamma by resting peripheral blood mononuclear cells ( PBMC ), and IL-12 mediated lymphokine activated killer cell activation
Kennedy et al., Eur J Immunol 1996 : Here, we show that the expression of CD40L by activated T cells is critical for T cell dependent IL-12 production by mouse macrophages ... Finally, macrophages cultured in the absence of T cells produced IL-12 upon stimulation with soluble recombinant CD40L in combination with either supernatants from activated Th1 clones or with interferon-gamma and granulocyte/macrophage colony stimulating factor ... Thus, both CD40L dependent and cytokine mediated signals from activated T cells are required to induce the production of IL-12 by macrophages
Blauvelt et al., J Clin Invest 1996 (Diseases in Twins) : IL-12 enhanced Ag-specific IL-2 production by TC from two patients with CD4+ counts > 600
Jeannin et al., J Immunol 1996 : Since IL-10 is a lymphokine associated with Th2 responses which decreases both IL-12 induced IFN-gamma production and IL-12 production by macrophages, we have analyzed here, in an APC-free system, the ability of IL-12 to modulate the production of human IL-10 by established Th0, Th1, and Th2 T cell clones (TCC), T cell lines, and purified peripheral blood T cells ... Moreover, using neutralizing anti-IL-2 Ab, we report that endogenous IL-2 produced by stimulated Th0 TCC could in part contribute to the effect of IL-12 on IL-10 and IL-4 production ... Moreover, using neutralizing anti-IL-2 Ab, we report that endogenous IL-2 produced by stimulated Th0 TCC could in part contribute to the effect of IL-12 on IL-10 and IL-4 production
Campbell et al., Immunity 1996 (Disease Susceptibility...) : Thus, a protective cell mediated immune response to L. major appears to be dependent upon CD40L induced IL-12 secretion by antigen presenting cells
Akridge et al., J Infect Dis 1996 (HIV Infections) : Surprisingly, exogenous IL-12 added to HIV infected macrophage cultures without autologous lymphocytes resulted in a 50 % -60 % reduction in RT activity and no detectable increase in IFN-gamma
Stuber et al., J Exp Med 1996 (Colitis...) : The recent finding that CD40L on activated T cells induces interleukin (IL) 12 secretion in human peripheral blood monocytes in vitro suggests that the CD40L-CD40 interaction may be of importance in the priming of T helper ( Th ) 1-type T cells ... In conclusion, we demonstrated that the CD40L-CD40 interaction is crucial for the in vivo priming of Th1 T cells via the stimulation of IL-12 secretion by antigen presenting cells (APC)
Rieckmann et al., J Neuroimmunol 1996 (Autoimmune Diseases...) : Increasing doses of PTX significantly reduced TNF-alpha and IL-12 mRNA expression of blood mononuclear cells, but increased IL-4 and IL-10 expression in eight patients with relapsing remitting multiple sclerosis
Annunziato et al., FASEB J 1996 : Surface LAG-3 expression correlated with IFN-gamma, but not IL-4, production in antigen stimulated T cells and it was up-regulated by IL-12
Li et al., Cell Immunol 1996 : This conclusion is clearly supported by the results that IL-12 indeed induced IFN-gamma production by activated B cells ... These results suggest that the B cell proliferative effect of IL-12 may be mediated by autocrine IFN-gamma
Kapsenberg et al., Int Arch Allergy Immunol 1996 (Hypersensitivity, Immediate) : The production of IL-12 and prostaglandin E2 is induced by the interaction between CD40 and CD40 ligand expressed by Th cells, and by biologically active agents such as micro-organisms or their products ... The production of IL-12 and prostaglandin E2 is induced by the interaction between CD40 and CD40 ligand expressed by Th cells, and by biologically active agents such as micro-organisms or their products
Gracie et al., Eur J Immunol 1996 : The observed alterations in alloantibody response were dependent, in large part, on the stimulatory effect of IL-12 on interferon (IFN)-gamma production by T lymphocytes and natural killer cells, since they were abrogated by co-administration of neutralizing anti-IFN-gamma monoclonal antibody following alloantigen immunization
Orange et al., J Immunol 1996 (Cytomegalovirus Infections...) : MCMV induces IFN-alphabeta dependent NK cell cytotoxicity and IL-12 dependent NK cell IFN-gamma production ... Examination of NK cell cytotoxicity, blastogenesis, and IFN-gamma production demonstrated that : IL-12 was required for NK cell IFN-gamma production but not blastogenesis and cytotoxicity ; IFN-alphabeta was necessary for NK cell blastogenesis and cytotoxicity but not IFN-gamma production ; and TNF facilitated IFN-gamma production but inhibited NK cell cytotoxicity
Fantuzzi et al., J Virol 1996 : Induction of interleukin-12 (IL-12) by recombinant glycoprotein gp120 of human immunodeficiency virus type 1 in human monocytes/macrophages : requirement of gamma interferon for IL-12 secretion ... We studied the effects of the gp120 glycoprotein of human immunodeficiency virus type 1 on the expression of interleukin-12 (IL-12) in human monocytes and in monocyte derived macrophages ... These data indicate that gp120 is an inducer of IL-12 expression in monocytes/macrophages and that IFN-gamma is an essential cofactor for IL-12 secretion, especially in differentiated macrophages
Jansen et al., Blood 1996 (Bacteremia...) : Lower IL-12 concentrations in the lethal group may have resulted in part from the enhanced production of IL-10 , a known inhibitor of IL-12 synthesis in vitro, as peak levels of this cytokine 3 hours postchallenge inversely correlated with peak levels of IL-12, in particular p40 ( r = -0.802 ; P < .01 ) ... Furthermore, this study indicates that the systemic release of IL-12 might be essential, but is not likely sufficient , to promote lethal production of IFN-gamma in sepsis
Karp et al., Science 1996 : Cross linking of CD46 , a complement regulatory protein that is the cellular receptor for MV, with antibody or with the complement activation product C3b similarly inhibited monocyte IL-12 production, providing a plausible mechanism for MV-induced immunosuppression
Klein-Franke et al., Anticancer Res 1995 : Activation of NK cell cytotoxicity and monocyte cytotoxicity by IL-12 were independent of exogenous IL-2 and IFN gamma ... Activation of NK cell cytotoxicity and monocyte cytotoxicity by IL-12 were independent of exogenous IL-2 and IFN gamma
Horvath-Arcidiacono et al., Int Immunol 1996 : Thus, effector T cells in IL-12 treated mice were not impaired, but exhibited augmented responsiveness, suggesting that IL-12 induced complex interactions among spleen cell populations and that these effects, in part, are mediated by IFN-gamma
Cleveland et al., Infect Immun 1996 : While the lipopolysaccharide (LPS) of gram negative bacteria induces monocyte production of IL-12 , the relevant gram positive components which induce IL-12 production are uncharacterized ... In contrast, lipoteichoic acid (LTA) , a predominant surface glycolipid of gram positive bacteria, potently induced IL-12 p40 gene expression ... A competitive LPS antagonist, Rhodobacter sphaeroides LPS , inhibited LTA induced IL-12 production, suggesting a common pathway for LPS and LTA in IL-12 activation ... A competitive LPS antagonist, Rhodobacter sphaeroides LPS, inhibited LTA induced IL-12 production, suggesting a common pathway for LPS and LTA in IL-12 activation
Daftarian et al., J Immunol 1996 : IL-10 production is enhanced in human T cells by IL-12 and IL-6 and in monocytes by tumor necrosis factor-alpha ... Similarly, IL-12 and IL-6 induced IL-10 production by purified CD4+ and CD8+ T cells ... IL-12 and/or IL-6 can induce the expression of IL-10 by PHA stimulated T cells, whereas TNF-alpha induces IL-10 production by monocytes ... IL-12 and/or IL-6 can induce the expression of IL-10 by PHA stimulated T cells, whereas TNF-alpha induces IL-10 production by monocytes ... Since IL-10 inhibits the production of IL-6, IL-12 , and TNF-alpha, these results may indicate a potential mechanism of negative feedback regulation of the immune response
Yu et al., J Immunol 1996 : In contrast to IL-2 and IFN-alpha, IL-12 induced strong tyrosine phosphorylation of STAT4 and variable weak phosphorylation of STAT3 ... In contrast to IL-2 and IFN-alpha, IL-12 induced strong tyrosine phosphorylation of STAT4 and variable weak phosphorylation of STAT3 ... However, supershift analyses using the c-fos promoter sis-inducible element probe showed that IL-12 activated STAT4, STAT1 alpha, and STAT3 , and induced complexes containing STAT4 only, STAT4 with STAT1 alpha, STAT3 with STAT1 alpha, or STAT1 alpha only in preactivated primary NK cells ... However, supershift analyses using the c-fos promoter sis-inducible element probe showed that IL-12 activated STAT4 , STAT1 alpha, and STAT3, and induced complexes containing STAT4 only, STAT4 with STAT1 alpha, STAT3 with STAT1 alpha, or STAT1 alpha only in preactivated primary NK cells ... STAT1 alpha activation by IL-12 correlated with increased phosphorylation of serine, but not tyrosine ... Finally, IL-2 induced tyrosine phosphorylation of JAK1 and JAK3, while IL-12 induced phosphorylation of JAK2 and TYK2 in both preactivated primary NK and NK3.3 cells ... Finally, IL-2 induced tyrosine phosphorylation of JAK1 and JAK3, while IL-12 induced phosphorylation of JAK2 and TYK2 in both preactivated primary NK and NK3.3 cells
Kincy-Cain et al., J Immunol 1996 (Disease Susceptibility...) : Successful resolution of salmonellosis in naive mice depends in large part upon IL-12 induced IFN-gamma production to eliminate this intracellular pathogen of macrophages
Fulton et al., Infect Immun 1996 (Tuberculosis) : Functionally, IL-12 readily enhanced PPD stimulated IFN-gamma production and CD4+ T-cell mediated cytotoxicity by peripheral blood mononuclear cells from healthy tuberculin positive donors but induced less enhancement when live M. tuberculosis was the antigen
Bhardwaj et al., J Clin Invest 1996 : IL-12 augmented interferon-gamma production during CTL generation
Thierfelder et al., Nature 1996 : IL-12 is required for the T-cell independent induction of the cytokine interferon (IFN)-gamma , a key step in the initial suppression of bacterial and parasitic infections ... To determine the function of Stat4 and its role in IL-12 signalling, we have produced mice that lack Stat4 by gene targeting
Sanchez-Bueno et al., Biochem Biophys Res Commun 1996 (Fibrosarcoma) : The iNOS, IL-12 , and IGIF expression was also impaired in bacteria activated Mos from IFN-gamma-/-mice, indicating that IFN-gamma, but not IGIF, would be the initial signal that leads to the activation of Mos in vivo
Lee et al., Blood 1996 : Decreased interleukin-12 (IL-12) from activated cord versus adult peripheral blood mononuclear cells and upregulation of interferon-gamma , natural killer, and lymphokine activated killer activity by IL-12 in cord blood mononuclear cells ... We compared the expression and production of IL-12 from activated cord versus adult mononuclear cells ( MNC ), regulatory mechanisms associated with IL-12 expression in CB MNC, and the effects of IL-12 on induction of CB interferon (IFN)-gamma production, NK, and lymphokine activated killer ( LAK ) cytotoxicity ... IL-12 mRNA expression was induced within 6 hours with LPS ( 10 micrograms/ml ) and reached peak levels at 12 hours in both CB and APB MNC ... Exogenous IL-12 ( 10 U/mL ) induced a significant increase of IFN-gamma from both CB and APB MNC ( 24 hours, 72 hours, P < .05, n = 3 ) ... The impaired, ability of CB MNC to produce IL-12 in response to stimulation may contribute to the decrease in IFN-gamma production and NK cytotoxicity ... However, IL-12 enhanced IFN-gamma and NK activity in CB MNC up to the comparable levels of APB MNC
Xiao et al., J Hematother 1996 : To understand the possible mechanisms for this suppression, ELISA assays were used to detect the release of interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha , which could potentially have been induced by IL-12 from CD34 cells ... To understand the possible mechanisms for this suppression, ELISA assays were used to detect the release of interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha, which could potentially have been induced by IL-12 from CD34 cells ... The results suggest that the suppressive effects of IL-12 on retroviral gene transduction are, at least in part, mediated by IL-12 induction of the release of TNF-alpha
Trinchieri et al., Ciba Found Symp 1995 : Early in infection, IL-12 acts as a proinflammatory cytokine and induces IFN-gamma production by NK and T cells ... IFN-gamma activates the phagocytes and increases their ability to produce IL-12
Zídek et al., J Interferon Cytokine Res 1996 : Antibody neutralization studies indicated that the effect of IL-12 was mediated by IFN-gamma without apparent participation of TNF-alpha
Goedert et al., J Immunol 1996 : On the contrary, the secondary IL-4 production of HD-CD4+ T cells as an indicator of Th2 differentiation, which was promoted by anti-CD4 mAb, was enhanced even in the presence of IL-12
Gupta et al., J Immunol 1996 : IL-12 is a potent inducer of IFN-gamma production by T cells and NK cells, and is thought to play a critical role in determining the nature of the local immune response to infection ... We here report that highly purified peritoneal mast cells from Brown Norway rats will produce IFN-gamma in response to IL-12 without significant histamine release ... We conclude that mast cells are capable of contributing to the IFN-gamma response to IL-12 , but substantial mast cell IFN-gamma production does not occur as a result of IgE mediated activation
Windhagen et al., J Immunol 1996 : Moreover, IL-12 enhanced IL-10 and IFN-gamma production in a myelin basic protein-reactive T cell clone, demonstrating that a differentiated T cell clone could be induced to secrete both cytokines ... Moreover, IL-12 enhanced IL-10 and IFN-gamma production in a myelin basic protein-reactive T cell clone, demonstrating that a differentiated T cell clone could be induced to secrete both cytokines ... Thus, in human T cells, IL-12 induces concomitant secretion of IL-10 and IFN-gamma ... Thus, in human T cells, IL-12 induces concomitant secretion of IL-10 and IFN-gamma
Yoo et al., J Immunol 1996 : HIV-1 infection induced IL-10 production in the macrophage hybridoma cell line with loss of IL-12 1 wk after infection
Bianchi et al., J Immunol 1996 (Hypersensitivity, Delayed...) : In vitro and in vivo cell depletion experiments suggested the following : 1 ) the exogenous IL-12 required both CD4+ and CD8+ cells for activity ; 2 ) the immune response initiated by IL-12 relied on later production of IL-12 by the host ; and 3 ) the early adjuvanticity of the exogenous IL-12 involved improved recognition of class II-restricted epitopes of this otherwise poorly immunogenic tumor peptide
Cella et al., J Exp Med 1996 : We found that ligation of CD40 by CD40L triggers the production of extremely high levels of bioactive IL-12
Armant et al., Eur J Immunol 1996 : Our present data demonstrate that the IL-2 induced IFN-gamma secretion, in the presence but also in the absence of sCD23, is strictly IL-12 dependent , inasmuch as anti-IL-12 antibody abrogated both responses ... Most interestingly, anti-CD40 ligand (CD40L) monoclonal antibody significantly inhibited IL-2 induced IL-12 as well as IFN-gamma production ... It is proposed that low levels of CD40L expression on non-antigen stimulated T cells are sufficient to signal through CD40 molecules on accessory cells and to induce IL-12 secretion, which in turn can synergize with IL-2 for the induction of IFN-gamma production, thus contributing to the inflammatory process
Micallef et al., Eur J Immunol 1996 : IGIF, but not IL-12 , significantly enhanced IL-2 and GM-CSF production in T cell cultures, as determined by CTLL-2 bioassay and ELISA, respectively ; however, both IGIF and IL-12 enhanced IFN-gamma production by the T cells ... IGIF, but not IL-12 , significantly enhanced IL-2 and GM-CSF production in T cell cultures, as determined by CTLL-2 bioassay and ELISA, respectively ; however, both IGIF and IL-12 enhanced IFN-gamma production by the T cells
Giese et al., Immunology 1996 (Murine Acquired Immunodeficiency Syndrome) : Induction of IFN-gamma by IL-12 was potentiated by TNF-alpha following stimulation of intact spleen cells and purified CD4+ or CD8+ T cells, as well as negatively selected CD4-8- cells from infected B6 mice
Seder et al., J Immunol 1996 : Similarly, addition of a relatively high dose of IL-2 also produced a significant enhancement of IFN-gamma production ; however, as previously reported, the presence of IL-12 in priming cultures induced the greatest increase in IFN-gamma production, leaving it as the predominant controller of Th1 differentiation in physiologic situations
Seitz et al., Eur J Immunol 1996 : In contrast, IL-12 stimulated the production of IL-1 beta and MCP-1 in TNF-alpha stimulated MNC and inhibited IL-1ra synthesis in cytokine stimulated cells ... In contrast, IL-12 stimulated the production of IL-1 beta and MCP-1 in TNF-alpha stimulated MNC and inhibited IL-1ra synthesis in cytokine stimulated cells ... In contrast, IL-12 stimulated the production of IL-1 beta and MCP-1 in TNF-alpha stimulated MNC and inhibited IL-1ra synthesis in cytokine stimulated cells
Kichian et al., J Immunol 1996 (Acute Disease...) : We have investigated whether IL-12 mediates the continued production of IFN-gamma during the state of T cell immunosuppression that accompanies aGVHD ... These results provide evidence for localized production of IFN-gamma within aGVHD target organs and suggest that it is mediated by LPS induced production of IL-12 by M phi
Mori et al., Int J Cancer 1996 (Body Weight...) : IL-12 reduced the serum levels of IL-6 , a cachexia mediator in this model, and alleviated the body weight loss and other abnormalities associated with cachexia, such as adipose tissue wasting and hypoglycemia ... IL-12 greatly increased levels of IFN-gamma in the tumor tissue and, to a lesser extent, in the circulation ... In athymic mice bearing the same colon 26 tumor, IL-12 was no longer anticachectic and did not induce IFN-gamma ... These results indicate that the anticachectic activity of IL-12 is T-cell dependent and results from at least 2 mechanisms, the down-regulation of IL-6 and the up-regulation of IFN-gamma ... These results indicate that the anticachectic activity of IL-12 is T-cell dependent and results from at least 2 mechanisms, the down-regulation of IL-6 and the up-regulation of IFN-gamma
Becher et al., J Clin Invest 1996 : IL-12 production is preceded by TNF-alpha production and is inhibited by recombinant soluble human TNF receptor ( II ) -IgG1 fusion protein ( shu-TNF-R )
Cantorna et al., J Infect Dis 1996 (Arthritis, Infectious...) : Earlier and stronger interleukin (IL)-12 , interferon-gamma (IFN)-gamma, and tumor necrosis factor responses were found in Borrelia burgdorferi infected, vitamin A-deficient mice compared with controls
Azzoni et al., J Immunol 1996 : Analysis of the expression of genes reported to regulate cytokine induced proliferation demonstrated that both IL-2 and IL-12 induce c-myc mRNA accumulation in NK and T cells, whereas only IL-2 induces bcl-2 expression
Noguchi et al., Proc Natl Acad Sci U S A 1996 (Skin Neoplasms) : Interferon gamma, IL-10, and tumor necrosis factor could be induced throughout the treatment period by IL-12 , indicating that repeated injections of IL-12 do not induce a state of tachyphylaxis ... Interferon gamma , IL-10, and tumor necrosis factor could be induced throughout the treatment period by IL-12 , indicating that repeated injections of IL-12 do not induce a state of tachyphylaxis ... Interferon gamma, IL-10 , and tumor necrosis factor could be induced throughout the treatment period by IL-12 , indicating that repeated injections of IL-12 do not induce a state of tachyphylaxis
Goldstein et al., Semin Immunol 1996 (Disease Models, Animal...) : The peripheral blood mononuclear cells were responsive to in-vivo cytokine regulation as evidenced by induction of human IFN-gamma gene expression by human IL-12 and inhibition by human IL-10
Scharton-Kersten et al., J Immunol 1996 (Peritonitis...) : Together, these data identify distinct functions for IL-12 and IFN-gamma in host resistance to T. gondii : IL-12 precedes and initiates synthesis of IFN-gamma , while the latter lymphokine directly controls parasite growth and diminishes the contribution of IL-4- and IL-5 producing T cell subsets
Venkataraman et al., Cryobiology 1996 : In view of the potent inhibitory properties of IL-10 on IL-12 secretion and the central role played by IL-12 in the immune system, the influences of freezing and radiation on LPS induced IL-12 production by PBMCs were studied ... These results suggest that the endogenously hyperscreted IL-10 is primarily responsible for the observed decrease in IL-12 released by the frozen cells
Guillaume et al., Bone Marrow Transplant 1996 : Culture of both control and transplant derived PBMC in PHA + IL-12 ( 20 U/ml ) or IL-2 ( 100 U/ml ) + IL-12 ( 20 U/ml ) combinations yielded comparable production of IFN-gamma, one of the major biological effects of IL-12 in vivo, as well as equal production of TNF-alpha, a costimulatory factor of IL-12 mediated induction of IFN-gamma by NK cells
Elenkov et al., Proc Assoc Am Physicians 1996 : To examine the potential effects of stress on TH1/TH2 balance, we studied the ability of three prototype stress hormones-dexamethasone ( a synthetic glucocorticoid ) and the catecholamines norepinephrine and epinephrine-to alter the production of IL-12 ( p70 ) and IL-10 induced by bacterial lipopolysaccharide (LPS) in human whole blood ... Dexamethasone inhibited LPS induced bioactive IL-12 production in a dose dependent fashion and at physiologically relevant concentrations ; it had no effect on IL-10 secretion
Hilkens et al., J Immunol 1996 : The majority of these TCC exhibited a strongly polarized Th2 cytokine profile, and the production of IFN-gamma could not be induced by exogenous IL-12 ... The IL-12 nonresponsiveness of the Th2 clones was further evident by the total lack of IL-12 induced phosphorylation of STAT4 ( signal transducer and activator of transcription-4 ), a transcription factor that is typically involved in IL-12 signaling
Heinzel et al., J Immunol 1996 (Endotoxemia) : Pretreatment of IFN-gamma 0/0 mice with recombinant mouse IFN-gamma ( rIFN-gamma ) enhanced circulating TNF-alpha by as much as sixfold, but serum IL-12 p40 and IL-12 p70 responses increased by only twofold or less ... Pretreatment of IFN-gamma 0/0 mice with recombinant mouse IFN-gamma ( rIFN-gamma ) enhanced circulating TNF-alpha by as much as sixfold, but serum IL-12 p40 and IL-12 p70 responses increased by only twofold or less ... The addition of rIFN-gamma to IFN-gamma 0/0 splenocyte culture restored normal levels of LPS stimulated IL-12 p40 production ... These data suggest that the aggregate IL-12 p40 and p70 response to endotoxemia in vivo is IFN-gamma independent and distinct from IFN-gamma dependent serum TNF-alpha and splenic IL-12 responses
Chensue et al., J Immunol 1996 (Granuloma) : In addition, MCP-1 inhibited IL-12 production by inflammatory macrophages ... In addition, MCP-1 inhibited IL-12 production by inflammatory macrophages
Okuno et al., Hepatogastroenterology 1996 (Liver Neoplasms) : These results demonstrate that IL-12 augments the cytolytic activity of HSL and suggests this cytokine as an attractive choice for liver metastases therapy
Iwamoto et al., Am J Respir Crit Care Med 1996 : Therefore, to determine whether IL-12 regulates antigen induced eosinophil recruitment into the airways, we studied the effect of recombinant murine IL-12 on antigen induced eosinophil infiltration into the tracheas of sensitized mice, and also the effect of IL-12 on IL-5 and interferon-gamma (IFN-gamma) levels in bronchoalveolar lavage fluid ( BALF ) from the mice ... Therefore, to determine whether IL-12 regulates antigen induced eosinophil recruitment into the airways, we studied the effect of recombinant murine IL-12 on antigen induced eosinophil infiltration into the tracheas of sensitized mice, and also the effect of IL-12 on IL-5 and interferon-gamma (IFN-gamma) levels in bronchoalveolar lavage fluid ( BALF ) from the mice
Gieni et al., Int Immunol 1996 : Differential production of IL-12 in BALB/c and DBA/2 mice controls IL-4 versus IFN-gamma synthesis in primed CD4 lymphocytes
Salvucci et al., Eur J Immunol 1996 : The differential effect of IL-4 on NK cell activation by IL-12 and IL-15 correlates with its ability to increase or to down-regulate the level of tumor necrosis factor-alpha and interferon-gamma release by NK cells, respectively
Scharton-Kersten et al., Exp Parasitol 1996 (Toxoplasmosis, Animal) : Together, our results suggest that ts-4 induces IL-12 dependent and -independent IFN-gamma production in normal mice, but ME49 induces the latter cytokine only in dependence upon IL-12
Abrahamsohn et al., Exp Parasitol 1996 (Chagas Disease...) : Spleen cells from anti-IL 12-treated WT mice had diminished production of IFN-gamma and NO, suggesting that early IFN-gamma synthesis is most dependent on IL-12 stimulation
Hedges et al., Cytokine 1996 : IL-4, IL-13, IFN-gamma, and TGF-beta 1, but not IL-2, IL-5, IL-10 or IL-12 , stimulated IL-6 secretion ... IL-5, IL-12 , IL-13 and TGF-beta 1 additively enhanced the bacterially induced IL-6 secretion, but they did not affect IL-8 secretion ... IL-5, IL-12 , IL-13 and TGF-beta 1 additively enhanced the bacterially induced IL-6 secretion, but they did not affect IL-8 secretion
Rabinovitch et al., J Autoimmun 1996 (Diabetes Mellitus, Type 1) : Because interleukin 12 (IL-12) is a potent inducer of IFN-gamma production, we sought evidence implicating IL-12 in IDDM development
Cho et al., J Immunol 1996 : Activation of STAT4 by IL-12 and IFN-alpha : evidence for the involvement of ligand induced tyrosine and serine phosphorylation ... In this study, we demonstrated that STAT4 activation by IL-12 is not unique ; IL-12 and IFN-alpha, but not IFN-gamma, induced tyrosine phosphorylation and DNA binding of STAT4 ... Thus, STAT4 activation was not IL-12 specific, and IL-12 and IFN-alpha activated STAT4 through both tyrosine and serine phosphorylation ... Thus, STAT4 activation was not IL-12 specific, and IL-12 and IFN-alpha activated STAT4 through both tyrosine and serine phosphorylation
Matsuoka et al., J Immunol 1996 : On the contrary, co-incubation with neutralizing Abs to IFN-gamma and IFN-gamma receptor did not affect IL-12 production, indicating that increased production of IL-12 stimulated by the analogue peptide was not due to an effect of IFN-gamma from T cells
Hanagiri et al., Cancer Immunol Immunother 1996 (Lung Neoplasms) : Furthermore, in the presence of hydrocortisone, IL-12 synergistically enhanced the cytotoxic activity of RLNL cultured with a high dose of IL-2
Metzger et al., Ann N Y Acad Sci 1996 : Initially, IL-12 induces production of IFN-gamma from Th1 and NK cells ... This step is not mediated by IFN-gamma but might be due to direct IL-12 binding to activated B lymphocytes
Kelsall et al., Ann N Y Acad Sci 1996 (Colitis) : In addition, stimulation of CD40 on human monocytes results in IL-12 production, suggesting that activated T cells expressing CD40L may directly induce the production of IL-12 by antigen presenting cells, thus allowing for the generation of a Th1 T-cell response in the absence of intracellular pathogens ... Finally, we show that IL-10 has a potent capacity to shut down CD40 induced IL-12 p40 secretion ; and, in addition, IL-4 partially inhibits CD40 induced IL-12 p40 secretion and enhances IL-10 mediated inhibition in an additive fashion ... Finally, we show that IL-10 has a potent capacity to shut down CD40 induced IL-12 p40 secretion ; and, in addition, IL-4 partially inhibits CD40 induced IL-12 p40 secretion and enhances IL-10 mediated inhibition in an additive fashion ... In conclusion, these findings suggest that DCs produce IL-12 in response to CD40 signaling, that a mechanism by which IL-4 may induce Th2 development is by acting with IL-10 to inhibit IL-12 production by DCs, and that the CD40L-CD40 interaction is crucial for the IL-12 dependent priming of Th1 T cells in vivo
Angiolillo et al., Ann N Y Acad Sci 1996 (Burkitt Lymphoma...) : IL-12 is an inducer of IFN-gamma and indirectly of IP-10 , an IFN-gamma-inducible protein, raising the possibility that the antiangiogenic effect of IL-12 is mediated by IP-10 ... IL-12 is an inducer of IFN-gamma and indirectly of IP-10, an IFN-gamma-inducible protein, raising the possibility that the antiangiogenic effect of IL-12 is mediated by IP-10
Quesniaux et al., Ann N Y Acad Sci 1996 : We investigated whether the in vivo inhibition of hematopoiesis by IL-12 is indirectly mediated by IL-12 induced IFN-gamma ... Thus, the reduction in total bone marrow cells and hematopoietic progenitor numbers following IL-12 treatment are largely indirect, mediated by IL-12 induced IFN gamma
Sher et al., Ann N Y Acad Sci 1996 (Granuloma...) : These effects appears to rely on the induction of IFN-gamma by the injected IL-12
Rook et al., Ann N Y Acad Sci 1996 (Lymphoma, T-Cell, Cutaneous...) : Because IL-12 is a potent inducer of IFN-gamma production and causes the activation of cytotoxic lymphocytes, we assessed the production of IL-12 by PBMCs from SzS patients, and whether IL-12 could alter the unfavorable cytokine balance typical of SzS and, thus, possibly lead to correction of immune defects
Presky et al., Ann N Y Acad Sci 1996 : Mouse IL-12 p40 subunit homodimer ( mo ( p40 ) 2 ) blocked 125I-huIL-12 binding to human IL-12R beta 1 , but did not inhibit binding to human IL-12R beta 2 ... Mouse IL-12 p40 subunit homodimer ( mo ( p40 ) 2 ) blocked 125I-huIL-12 binding to human IL-12R beta 1, but did not inhibit binding to human IL-12R beta 2
Maruo et al., J Immunol 1997 : B cells regulate CD40 ligand induced IL-12 production in antigen presenting cells (APC) during T cell/APC interactions ... IL-12 production was dependent on the presence of CD4+ , but not of CD8+, T cells, and inhibited by addition of anti-CD40 ligand (CD40L) mAb ... IL-12 production was dependent on the presence of CD4+, but not of CD8+ , T cells, and inhibited by addition of anti-CD40 ligand (CD40L) mAb ... IL-12 production was also induced by interactions between CD40L transfected Chinese hamster ovary cells and splenocytes depleted of T and B cells, but not of APC, indicating CD40L induced IL-12 production by APC ... These results indicate that CD40L expressed on activated T cells induces APC to produce IL-12 through CD40/CD40L interaction, but this pathway is competitively inhibited by CD40+ B cells incapable of producing IL-12 upon stimulation with CD40L ... These results indicate that CD40L expressed on activated T cells induces APC to produce IL-12 through CD40/CD40L interaction, but this pathway is competitively inhibited by CD40+ B cells incapable of producing IL-12 upon stimulation with CD40L
Handel-Fernandez et al., J Immunol 1997 (Mammary Neoplasms, Experimental) : Down-regulation of IL-12 , not a shift from a T helper-1 to a T helper-2 phenotype, is responsible for impaired IFN-gamma production in mammary tumor bearing mice
DeKruyff et al., J Immunol 1997 : IL-12 production in this T cell dependent system increased in direct proportion to Ag concentration and required TCR ligation but not CD28 costimulation ... In this second pathway, IL-12 production was completely independent of CD40 triggering ... In both pathways, the presence of IFN-gamma was not required for induction of IL-12 synthesis when splenic adherent cells ( SAC ) from normal mice were used
Harrison et al., J Immunol 1997 (Acquired Immunodeficiency Syndrome...) : Because of recent studies demonstrating that IFN-gamma priming increases the production of IL-12 in normal PBMC, we examined the role of IFN-gamma in the production of IL-12 in PBMC from HIV-seropositive donors
McDyer et al., J Immunol 1997 (Tuberculosis, Multidrug-Resistant) : IL-12 also caused a striking increase in the amount of IFN-gamma produced from PBMCs of both PPD positive and -negative controls
Ueta et al., Eur J Immunol 1996 : Recombinant human IL-12 stimulated gammadelta T cells, but not alphabeta T cells in peripheral blood mononuclear cells, to express CD25 on their surfaces, and to expand in number in vitro ... Anti-TNF-alpha monoclonal antibody inhibited IL-12 induced up-regulation of CD25 on gammadelta T cells, suggesting that endogenous TNF-alpha may play a role in IL-12 induced activation of gammadelta T cells ... Furthermore, IL-12 up-regulated TNF receptors on gammadelta T cells in vitro : TNF-alpha binding to its receptor induced CD25 expression on the gammadelta T cells in an autocrine or paracrine fashion, or perhaps both ... Furthermore, IL-12 up-regulated TNF receptors on gammadelta T cells in vitro : TNF-alpha binding to its receptor induced CD25 expression on the gammadelta T cells in an autocrine or paracrine fashion, or perhaps both
Vezzio et al., Int Immunol 1996 : We found that : ( I ) DC but not monocytes released IL-12 and promoted IL-12 dependent IFN-gamma production upon interaction with anti-CD3- or IL-2 stimulated Th2C and (II) ligation of CD3 was required for the production of IL-4 but not of IL-5 or IFN-gamma
Urban et al., Vet Immunol Immunopathol 1996 (Cryptosporidiosis...) : At the molecular level, exogenous IL-12 stimulates IFN-gamma production which elevates a protective type 1 response to C. parvum but converts the normally anti-worm type 2 response to a type 1 response that inappropriately regulates the infection
Sartori et al., Methods 1997 (HIV Infections) : IL-12 induces production of cytokines, especially interferon-gamma , from both T and NK cells, enhances the cytotoxic activity of NK cells and the generation of cytotoxic T cells, and has a proliferative activity on T and NK cells
Enk et al., Photochem Photobiol 1996 : Because IL-12 induces production of TNF-alpha by certain cells of the immune system, we sought to determine whether UVB is an inducer of IL-12 gene expression in epidermal cells
Ohshima et al., J Immunol 1997 : IL-12 production was suppressed by exogenous IL-4 and increased by anti-IL-4 blocking mAbs, indicating that endogenous IL-4 down-regulated IL-12 production by DC
Miller et al., Ann N Y Acad Sci 1996 (Listeriosis) : In light of what is known about the ability of IL-12 to induce IFN-gamma production by NK cells and gamma delta T cells, these results suggest that the exogenous addition of IL-12 may help initiate a cytokine cascade which enables the immune system to interact productively with an antigen that is typically nonimmunogenic when administered alone
Meyaard et al., Blood 1997 (HIV Infections) : IL-12 production by human monocytes is enhanced by interferon-gamma (IFN-gamma) and inhibited by IL-10 and prostaglandin E2 ( PGE2 ) ... In conclusion, IL-12 production by cells from HIV infected individuals is impaired under certain conditions in vitro and this decrease is independent of IL-10 or PGE2 production
Libraty et al., J Clin Invest 1997 (Leprosy) : In response to Mycobacterium leprae stimulation, IFN-gamma differentially regulated IL-12 and IL-10 production resulting in upregulation of IL-12 release and downregulation of IL-10 release
Frosch et al., Med Microbiol Immunol 1996 : IFN-gamma production by natural killer cells and T cells is induced by interleukin-12 (IL-12)
Balashov et al., Proc Natl Acad Sci U S A 1997 (Multiple Sclerosis) : We found that interleukin 12 (IL-12) was responsible for raised IFN-gamma secretion in MS as anti-IL-12 antibodies reversed raised anti-CD3 induced IFN-gamma in MS patients to normal levels
Cousens et al., Proc Natl Acad Sci U S A 1997 (Cytomegalovirus Infections...) : Both IFN-alpha and IFN-beta inhibited fixed Staphylococcus aureus Cowan strain induction of IL-12 and IFN-gamma production by mouse splenic leukocytes in culture
Wu et al., Eur J Immunol 1997 : Among 16 cytokines tested, IL-2, IL-7 and IL-15 markedly induced IL-12Rbeta1 expression and IL-12 binding on resting PBMC, whereas IL-1alpha and tumor necrosis factor-alpha had a minimal enhancing effect ... Among 16 cytokines tested, IL-2 , IL-7 and IL-15 markedly induced IL-12Rbeta1 expression and IL-12 binding on resting PBMC, whereas IL-1alpha and tumor necrosis factor-alpha had a minimal enhancing effect ... Among 16 cytokines tested, IL-2, IL-7 and IL-15 markedly induced IL-12Rbeta1 expression and IL-12 binding on resting PBMC, whereas IL-1alpha and tumor necrosis factor-alpha had a minimal enhancing effect
Ross et al., Blood 1997 (Lymphoma, Non-Hodgkin) : In summary, costimulation of human NK cells via the IL-2 receptor and the IL-12 receptor induces significant IFN-gamma production, followed by NK cell apoptosis and a decline in IFN-gamma production
Nakamura et al., J Immunol 1997 : The transient expression of CD30 on differentiating Th1 lineage cells was mainly the result of endogenously produced IL-4 induced by IL-12
Hunter et al., J Immunol 1997 (Mast-Cell Sarcoma...) : Since IL-12 induced production of IFN-gamma by NK cells is associated with resistance to certain infections, we examined whether the CD28/B7 interaction regulated NK cell responses during infection
Kincy-Cain et al., J Immunol 1997 : The costimulatory effects of substance P agonist plus LPS on IL-12 mRNA expression were additive ; however, this combination resulted in synergistic secretion of IL-12p70 by macrophages
Kornelisse et al., Infect Immun 1997 (Meningitis, Bacterial) : The production of IFN-gamma is induced by IL-12 with tumor necrosis factor alpha (TNF-alpha) as a costimulator and inhibited by IL-10 ... The production of IFN-gamma is induced by IL-12 with tumor necrosis factor alpha (TNF-alpha) as a costimulator and inhibited by IL-10
Trinchieri et al., Curr Opin Immunol 1997 (Infection) : If IL-12 and IL-12 induced IFN-gamma are present during early T cell expansion in response to antigen, Th1 cell generation is favored and the generation of Th2 cells is inhibited
Biron et al., Curr Opin Immunol 1997 (Virus Diseases) : Recent work has shown that some, but not all, viral infections induce IL-12 , the expression of which results in NK cell IFN-gamma production, and that NK cell IFN-gamma production contributes to an antiviral state ... IL-12 expression can be regulated by IFN-alpha/beta , and endogenous IFN-alpha/beta is responsible for the lack of IL-12 during viral infections that fail to elicit detectable production of this factor
Heike et al., Scand J Immunol 1997 (Sarcoma) : Contrary roles of IL-4 and IL-12 on IL-10 production and proliferation of human tumour reactive T cells ... In contrast, IL-12 substantially increased the production of IL-10 and IFN-gamma ... In contrast, IL-12 substantially increased the production of IL-10 and IFN-gamma
Marinaro et al., J Exp Med 1997 (Hypersensitivity, Delayed) : Further, serum IgE and S-IgA Ab responses were markedly reduced by parenteral IL-12
Nakamura et al., J Immunol 1997 : It has previously been reported that Th1 CD4 T cell populations can be converted to IL-4 producers, whereas Th2 populations are refractory to IL-12 mediated IFN-gamma production
Verhasselt et al., J Immunol 1997 : Experiments using neutralizing anti-IL-12 mAb indicated that LPS induced IL-12 is responsible for the increased production of IFN-gamma but not for the increased proliferation during MLR ... Experiments using neutralizing anti-IL-12 mAb indicated that LPS induced IL-12 is responsible for the increased production of IFN-gamma but not for the increased proliferation during MLR
Cavanaugh et al., J Virol 1997 : Importantly, we demonstrated that although IFN-gamma, tumor necrosis factor alpha, and IFN-alpha/beta mRNA are induced in the liver and kidney after IL-12 administration, the antiviral effect of IL-12 is mediated principally by its ability to induce IFN-gamma production in this model
Kato et al., Cell Immunol 1997 : IL-2 production of all T cell populations was not affected by IL-12 even in the presence of B7-2CHO
Ryffel et al., Clin Immunol Immunopathol 1997 (Anemia...) : Interleukin-12 : role of interferon-gamma in IL-12 adverse effects ... Investigations in mutant mice deficient for the IFN-gamma receptor ( IFN-gamma R-/- ) revealed that both the biologic and toxic effects are indirect and largely due to IL-12 induced IFN-gamma
Gollob et al., Eur J Immunol 1997 : One feature of Th2 differentiation in mice is the loss of IL-12 induced Jak2 and Stat4 activation, which is accompanied by the inability to produce IFN-gamma in response to IL-12 ... One feature of Th2 differentiation in mice is the loss of IL-12 induced Jak2 and Stat4 activation, which is accompanied by the inability to produce IFN-gamma in response to IL-12 ... In contrast, the enhancement of IL-12 responsiveness by IFN-gamma is associated with the upregulation of high-affinity IL-12 receptor expression ... Through the use of a newly synthesized neutralizing antibody to the low-affinity IL-12 receptor beta subunit ( IL-12Rbeta ), we show that neither IL-4 nor IFN-gamma affect the expression of IL-12Rbeta , which we determine to be one of at least two low-affinity subunits required for high-affinity IL-12 binding ... Through the use of a newly synthesized neutralizing antibody to the low-affinity IL-12 receptor beta subunit ( IL-12Rbeta ), we show that neither IL-4 nor IFN-gamma affect the expression of IL-12Rbeta , which we determine to be one of at least two low-affinity subunits required for high-affinity IL-12 binding ... These findings suggest that IL-4 and IFN-gamma exert opposite effects on T cell IL-12 responsiveness by differentially modulating the expression of low-affinity IL-12 receptor subunits that are distinct from IL-12Rbeta and required , together with IL-12Rbeta, for high-affinity IL-12 binding and IL-12 responsiveness ... These findings suggest that IL-4 and IFN-gamma exert opposite effects on T cell IL-12 responsiveness by differentially modulating the expression of low-affinity IL-12 receptor subunits that are distinct from IL-12Rbeta and required , together with IL-12Rbeta, for high-affinity IL-12 binding and IL-12 responsiveness
Hu-Li et al., Proc Natl Acad Sci U S A 1997 : CD4+ T cells from T cell receptor transgenic mice that have been vigorously primed to be interleukin (IL)-4 producers ( T ( H2 ) cells ) are capable of producing IL-4 even if restimulated in the absence of IL-4 and in the presence of IL-12 ... Cells primed in the absence of IL-4 become highly responsive to IL-12 if IFN-gamma is included in the priming culture, and these cells are excellent IFN-gamma producers upon challenge ; IL-12 considerably enhances their production of IFN-gamma ... Thereafter, they produce large amounts of IFN-gamma upon challenge with antigen in the presence of IL-12
Kakumu et al., Clin Exp Immunol 1997 (Chronic Disease...) : Addition of IL-10 to the cultures equally suppressed IFN-gamma production for both controls and patient groups, but the enhancing effect of IL-12 on IFN-gamma production was significantly less in LC than in controls and other patient groups
Monteleone et al., Gastroenterology 1997 (Colitis, Ulcerative...) : The heterodimer interleukin (IL)-12 , produced by phagocytes, induces T-cell cytokines, primarily interferon (IFN)-gamma
Chensue et al., Inflamm Res 1997 (Granuloma...) : Exogenous IL-12 profoundly augmented IFN-gamma and abrogated type 2 cytokines while inhibiting intrinsic IL-12 production in lymph nodes
Scott et al., Pediatr Res 1997 : IFN-gamma was also produced in the S. aureus stimulated cultures, in an IL-12 dependent manner, but cord blood MNC produced 5-fold lower levels of IFN-gamma compared with adult MNC ( p < 0.05 )
Ruzek et al., J Exp Med 1997 (Herpesviridae Infections) : The IL-12 p40, IFN-gamma, TNF, and IL-6 responses were dramatic with peak levels reaching > 150-10,000 pg/ml at 32-40 h after infection and rapidly declining thereafter
Yoshimoto et al., Proc Natl Acad Sci U S A 1997 : The major activity associated with this molecule is the induction of IFN-gamma production from anti-CD3 activated T helper 1 cells in the presence of IL-12 ... Furthermore, we show that, like splenic T cells stimulated with anti-CD3, IL-12, and IL-18, B cells produced high level of IFN-gamma in response to anti-CD40, IL-12 , and IL-18
Taoufik et al., Blood 1997 : We showed that in human monocyte cultures, HIV-gp120 induces a significant IL-10 synthesis, which in turn inhibits IL-12 subunits mRNA accumulation and protein secretion after SAC-activation ... We showed that in human monocyte cultures, HIV-gp120 induces a significant IL-10 synthesis, which in turn inhibits IL-12 subunits mRNA accumulation and protein secretion after SAC-activation
Chu et al., J Immunol 1997 (Lymphoma, B-Cell) : CD28+ and CD28- T cells differed, however, with respect to the effect of IL-12 on IL-4 production
Perry et al., J Immunol 1997 (Chlamydia Infections...) : To dissociate the protective role of IL-12 from that of IL-12 induced IFN-gamma , resistance to C. trachomatis was compared in IL-12 depleted and IFN-gamma-deficient animals
Tsung et al., J Immunol 1997 (Fibrosarcoma) : Examination of tumor associated T cells indicates that IL-12 induces production of the Th1 cytokine IFN-gamma and suppresses production of IL-2, IL-4, and IL-10 at the tumor site, where these are found to be the predominant cytokines produced by tumor associated T cells before IL-12 treatment ... Examination of tumor associated T cells indicates that IL-12 induces production of the Th1 cytokine IFN-gamma and suppresses production of IL-2 , IL-4, and IL-10 at the tumor site, where these are found to be the predominant cytokines produced by tumor associated T cells before IL-12 treatment ... Examination of tumor associated T cells indicates that IL-12 induces production of the Th1 cytokine IFN-gamma and suppresses production of IL-2, IL-4 , and IL-10 at the tumor site, where these are found to be the predominant cytokines produced by tumor associated T cells before IL-12 treatment ... Examination of tumor associated T cells indicates that IL-12 induces production of the Th1 cytokine IFN-gamma and suppresses production of IL-2, IL-4, and IL-10 at the tumor site, where these are found to be the predominant cytokines produced by tumor associated T cells before IL-12 treatment
Mahanty et al., Infect Immun 1997 (Elephantiasis, Filarial) : To assess whether IL-12 could reverse the type 1 downregulation observed, the effect of recombinant human IL-12 ( rhIL-12 ) on BMA-driven IL-5 and IFN-gamma production was also evaluated ... To assess whether IL-12 could reverse the type 1 downregulation observed, the effect of recombinant human IL-12 ( rhIL-12 ) on BMA-driven IL-5 and IFN-gamma production was also evaluated
Macaulay et al., J Immunol 1997 : These results suggest that B cells activated by their cognate Ag preferentially induce IL-4 synthesis as a result of the interaction of CD40L on T cells with CD40, whereas SAC preferentially induce IFN-gamma synthesis by T cells as a result of their greater production of IL-12 and their limited capacity to trigger CD40L on T cells ... These results suggest that B cells activated by their cognate Ag preferentially induce IL-4 synthesis as a result of the interaction of CD40L on T cells with CD40, whereas SAC preferentially induce IFN-gamma synthesis by T cells as a result of their greater production of IL-12 and their limited capacity to trigger CD40L on T cells ... These results suggest that B cells activated by their cognate Ag preferentially induce IL-4 synthesis as a result of the interaction of CD40L on T cells with CD40, whereas SAC preferentially induce IFN-gamma synthesis by T cells as a result of their greater production of IL-12 and their limited capacity to trigger CD40L on T cells
Heinzel et al., J Immunol 1997 (Endotoxemia) : To determine whether IL-12 p40 homodimer was capable of antagonizing IL-12 dependent IFN-gamma responses in vivo, we pretreated C57BL/6 mice with purified rIL-12 p40 homodimer before i.p. challenge with endotoxin
Lissoni et al., J Biol Regul Homeost Agents 1995 (Neoplasms) : On the contrary, IL-12 significantly diminished IL-10 release in response to IL-2, and this inhibitory effect was more pronounced when IL-2 was added in association with both IL-12 and MLT
Flesch et al., Int Immunol 1997 (Granuloma...) : Although direct activation of host macrophages by IL-13 is possible, we consider it more likely that IL-13 acted indirectly through stimulation of IL-12 production and inhibition of IL-4 release early after infection
van der Pouw Kraan et al., J Immunol 1997 (Asthma) : Reduced production of IL-12 and IL-12 dependent IFN-gamma release in patients with allergic asthma ... In line with the reduced levels of IL-12 secretion, the patient group showed a 3-fold reduction of IL-12 dependent IFN-gamma production ( p < 0.005 )
Sutterwala et al., J Exp Med 1997 : In this study, we examined the effects that ligating specific macrophage receptors had on the induction of IL-12 by lipopolysaccharide (LPS) ... Second, exposure of macrophages to the calcium ionophores, ionomycin or A23187, mimicked receptor ligation and inhibited IL-12 ( p40 ) mRNA induction by LPS ... These results indicate that the calcium influxes that occur as a result of receptor ligation are responsible for inhibiting the induction of IL-12 by LPS
Osugi et al., Blood 1997 (Histiocytosis, Non-Langerhans-Cell) : IL-12 was detected in plasma in all patients ( mean peak value, 30.0 +/- 5.0 pg/mL ), while IFN-gamma was massively produced in nine patients ( mean peak value, 79.2 +/- 112.0 U/mL )
Xiong et al., Clin Exp Immunol 1997 (Listeriosis) : Taking all facts into consideration, it may be concluded that persistent production of IFN-gamma induced by persistent production of IL-12 during immunizing infection is essential for the generation of protective immunity against L. monocytogenes
Spencer et al., Int Immunol 1997 : IL-12 directly stimulates expression of IL-10 by CD5+ B cells and IL-6 by both CD5+ and CD5- B cells : possible involvement in age associated cytokine dysregulation ... A role for IL-12 in the aging process was suggested when it was found that recombinant IL-12 ( rIL-12 ) directly stimulated splenic CD5+ B cells to secrete IL-10, and both CD5+ and CD5- B cells could be directly induced to produce IL-6 in response to rIL-12 ... A role for IL-12 in the aging process was suggested when it was found that recombinant IL-12 ( rIL-12 ) directly stimulated splenic CD5+ B cells to secrete IL-10, and both CD5+ and CD5- B cells could be directly induced to produce IL-6 in response to rIL-12 ... Based on these observations it was concluded that IL-12 might be responsible for the dysregulated production of IL-10 and IFN-gamma known to occur in aged animals ... Based on these observations it was concluded that IL-12 might be responsible for the dysregulated production of IL-10 and IFN-gamma known to occur in aged animals
Marzusch et al., Hum Reprod 1997 : Neither IL-12 nor IL-2 stimulated the secretion of IFN-gamma by decidual macrophages ... IL-12 alone, but not IL-2 alone, stimulated the release of IFN-gamma by LGL
Medvedev et al., Cytokine 1997 : IL-2 and IL-7 equally enhanced the Fas expression in the CD56+CD3+ population, while IL-12 had a less pronounced effect
Rudy et al., Int Immunol 1997 (Melanoma...) : Experimental evidence indicates that this inhibitory effect was mediated by IFN-gamma since ( I ) IFN-gamma secretion of stimulated T cells was augmented by IL-12 , ( II ) exogenous IFN-gamma also inhibited T cell proliferation induced by CD80- but not CD86 transfected SkMel63 cells and ( III ) the inhibitory effect of IL-12 was blocked by an anti-IFN-gamma mAb
Scott et al., J Immunol 1997 : Initial treatment with anti-IL-12 prevented BA-induced early IFN-gamma production and secondary IgG2a responses, but did not abrogate IgE suppression
Yamabe et al., Immunology 1997 : Induction of the 2B9 antigen/dipeptidyl peptidase IV/CD26 on human natural killer cells by IL-2, IL-12 or IL-15 ... These results showed that the 2B9 Ag is identical to CD26, and demonstrated that CD26 is an activation antigen on CD16+ CD56+ NK cells inducible by IL-2, IL-12 or IL-15
Fujiwara et al., Leukemia 1997 (Ovarian Neoplasms) : The anti-tumor effects of IL-12 involve enhanced IFN-gamma production by anti-tumor T cells, their accumulation to tumor sites and in situ IFN-gamma production
Keefe et al., Vet Immunol Immunopathol 1997 (Cattle Diseases...) : Consistently elevated levels of IL-2 and IL-12 in AL and PL cattle in pokeweed mitogen stimulated cells was detected , while IFN-gamma was elevated in the AL but not the PL cattle
Cassone et al., J Leukoc Biol 1997 (Acquired Immunodeficiency Syndrome...) : IL-12 was also produced by MP-F2 stimulated PMN in the presence of interferon-gamma (IFN-gamma)
Peng et al., Cytokine 1997 : In contrast, IL-4 and IL-5 production were slightly inhibited by IL-12 ... In contrast, IL-4 and IL-5 production were slightly inhibited by IL-12 ... The effect of IL-12 on the secretion of IL-10 was confirmed by stimulating T cells in the absence of accessory cells with immobilized anti-CD3 mAb and soluble anti-CD28 mAb ... On the other hand, IL-12 did not affect IL-2 production
Chace et al., Clin Immunol Immunopathol 1997 : Bacterial DNA induced NK cell IFN-gamma production is dependent on macrophage secretion of IL-12
Guéry et al., Eur J Immunol 1997 : B cells also fail to secrete IL-12 in response to stimulation with LPS and IFN-gamma ... B cells also fail to secrete IL-12 in response to stimulation with LPS and IFN-gamma
Fukumoto et al., Jpn J Cancer Res 1997 (Carcinoma, Lewis Lung) : The culture medium of the transfectant cells stimulated interferon-gamma (IFN-gamma) production by spleen cells in vitro in the presence of anti-CD3 antibody and markedly potentiated the effect of interleukin-12 (IL-12) on IFN-gamma production by spleen cells
Aloisi et al., J Immunol 1997 : Combined stimulation with IFN-gamma/LPS enhances IL-12 p40 secretion and induces IL-12 p75 secretion by microglia ... Combined stimulation with IFN-gamma/LPS enhances IL-12 p40 secretion and induces IL-12 p75 secretion by microglia ... Combined stimulation with IFN-gamma/LPS enhances IL-12 p40 secretion and induces IL-12 p75 secretion by microglia ... Combined stimulation with IFN-gamma/LPS enhances IL-12 p40 secretion and induces IL-12 p75 secretion by microglia ... IL-12 production by activated microglia is inhibited by IL-10 , PGE2, and cAMP elevating agents
Wu et al., J Immunol 1997 : To determine the role of IL-12Rbeta1 in mediating the biologic functions of IL-12 in mice, we have generated IL-12Rbeta1-deficient ( IL-12Rbeta1 ( -/- ) ) mice by targeted mutation in ES cells ... Unlike those from IL-12Rbeta1 ( +/+ ) mice, Con A-activated splenocytes from IL-12Rbeta1 ( -/- ) mice failed to proliferate or produce IFN-gamma in response to IL-12 , even at very high concentrations ( 67 nM )
Güler et al., J Immunol 1997 : The pattern of IL-12 responsiveness correlated with expression of the IL-12R signaling subunit, IL-12R beta2, and with IL-12 induced STAT4 phosphorylation
Minty et al., Eur Cytokine Netw 1997 : Indeed, IL-13 actually increases SAC induced IL-12 production ... Production of IL-12 , MCP-1, TNF-alpha and to a lesser extent IL-6 induced by LPS is now `` primed '', whereas that of IL-1, IL-8, and IL-10 is still inhibited ... The priming effect of IL-13 on IL-12 production is additive with that of IFN-gamma, and is partly independent of IFN-gamma
Deshpande et al., Am J Reprod Immunol 1997 : Our results show that 17 beta-estradiol decreases LPS induced IL-1 alpha, IL-6, and TNF-alpha production but not IL-10, IL-12 , and macrophage inflammatory protein (MIP) production by splenic macrophages
Pulendran et al., J Immunol 1997 : These DC subpopulations showed different capacities to phagocytose FITC-zymosan and to secrete IL-12 upon stimulation with Staphylococcus aureus cowan I strain + IFN-gamma + granulocyte-macrophage-CSF
Shibata et al., J Immunol 1997 : The treatments with soluble mannan or with cytochalasin D, in sharp contrast, did not inhibit LPS induced IL-12/IFN-gamma production or exogenous IL-12 induced IFN-gamma production ... The treatments with soluble mannan or with cytochalasin D, in sharp contrast, did not inhibit LPS induced IL-12/IFN-gamma production or exogenous IL-12 induced IFN-gamma production
Hilkens et al., Blood 1997 : Bacteria or bacterial compounds such as Staphylococcus aureus Cowan strain I ( SAC ) or lipopolysaccharide (LPS) induced substantial IL-12 levels in DC, which could be further upregulated by interferon-gamma (IFN-gamma), whereas induction of IL-12 production via CD40 ligation required IFN-gamma as an obligatory, complementary signal
Panina-Bordignon et al., J Clin Invest 1997 : In this report we present evidence that beta2-agonists inhibit IL-12 production by both human monocytes in response to lipopolysaccharide (LPS) and dendritic cells stimulated via CD40 ... IL-12 inhibition is dependent on beta2-adrenoceptor stimulation and correlates with increased levels of intracellular cAMP
Gurunathan et al., J Exp Med 1997 (Leishmaniasis, Cutaneous) : Moreover, both the enhancement of IFN-gamma production and the protective immune response induced by LACK DNA vaccination was IL-12 dependent
Komatsu et al., J Immunol 1997 (Rhabdoviridae Infections) : IFN-gamma is not required in the IL-12 response to vesicular stomatitis virus infection of the olfactory bulb
Puddu et al., J Immunol 1997 : IL-12 induces IFN-gamma expression and secretion in mouse peritoneal macrophages ... In this study, we investigated the effects of IL-12 on the replication of vesicular stomatitis virus and on IFN-gamma gene expression in mouse PM ... Moreover, IL-12 induced a consistent secretion of IFN-gamma , especially in cultured PM ... We suggest that IL-12 induced IFN-gamma production by macrophages can play some role in the generation of the antiviral and immunoregulatory effects of IL-12
Leonard et al., Blood 1997 (Colitis...) : We determined that a single injection of rhIL-12 2 weeks before consecutive dosing included in the Phase 1 study, but not in the schedule of administration in the Phase 2 study, has a profound abrogating effect on IL-12 induced interferon-gamma (IFN-gamma) production and toxicity
Li et al., Clin Exp Immunol 1997 (Herpesviridae Infections) : Interferon-gamma (IFN-gamma) regulates production of IL-10 and IL-12 in human herpesvirus-6 ( HHV-6 ) -infected monocyte/macrophage lineage ... Expression and production of IL-12 were markedly increased when anti-human IL-10 MoAbs were added to the cultures, implying that endogenous IL-10 induced by HHV-6 inhibited IL-12 production ... Determination of protein levels of IL-10 and IL-12 by ELISA also showed that IFN-gamma increased IL-12 and decreased IL-10 production ... These results suggest that IFN-gamma regulates the production of IL-10 and IL-12 at transcriptional level mainly through inhibiting endogenous IL-10 production in HHV-6 infected monocyte/macrophage lineage ... These results suggest that IFN-gamma regulates the production of IL-10 and IL-12 at transcriptional level mainly through inhibiting endogenous IL-10 production in HHV-6 infected monocyte/macrophage lineage
Yin et al., Arthritis Rheum 1997 (Arthritis, Reactive...) : Exogenous IL-12 clearly enhanced IFN gamma and TNF alpha secretion ... Exogenous IL-12 clearly enhanced IFN gamma and TNF alpha secretion
Kato et al., Cell Immunol 1997 : Differential effects of LPS and CD40 ligand stimulations on the induction of IL-12 production by dendritic cells and macrophages ... Differential effects of LPS and CD40 ligand stimulations on the induction of IL-12 production by dendritic cells and macrophages ... In this experiment, we have examined the IL-12 production of murine macrophages ( Mphi ) and dendritic cells ( DC ) in response to LPS and CD40 ligand (CD40L) stimulations ... In this experiment, we have examined the IL-12 production of murine macrophages ( Mphi ) and dendritic cells ( DC ) in response to LPS and CD40 ligand (CD40L) stimulations ... The results showed that the ligation of CD40 induced the enhancement of IL-12 p40 mRNA accumulation and IL-12 production in DC as well as in Mphi ; however, neither the accumulation of IL-12 p40 mRNA nor the production of bioactive IL-12 was detected in DC stimulated with various concentrations of LPS, although Mphi produced IL-12 on LPS stimulation
Peng et al., Cell Immunol 1997 : In the presence of IL-12 , the cytokine profile was shifted to high IFN-gamma and IL-10 production and IL-4 and IL-5 production were slightly inhibited ... In the presence of IL-12 , the cytokine profile was shifted to high IFN-gamma and IL-10 production and IL-4 and IL-5 production were slightly inhibited
Robinson et al., Immunity 1997 : IGIF does not drive Th1 development but synergizes with IL-12 for interferon-gamma production and activates IRAK and NFkappaB ... IGIF and IL-12 synergized for IFN gamma production from Th1 cells
Alexander et al., Philos Trans R Soc Lond B Biol Sci 1997 (Inflammation...) : Studies with gene-deficient mice have demonstrated the interleukin-12 (IL-12) dependent production of interferon gamma (IFN-gamma) to be of paramount importance in controlling early parasite growth ... Once initiated, IL-12 dependent IFN-gamma production in synergy with other proinflammatory cytokines can positively feed back on itself to induce ` cytokine shock '
Zhao et al., Antisense Nucleic Acid Drug Dev 1997 : One oligonucleotide ( sequence 5'-GAG AAC GCT CGA CCT TCG AT-3 ' ) induced a high level of IL-12 secretion even at 5 mg/kg, whereas another oligonucleotide ( sequence 5'-CTC TGC CAC CCA TCT CTC TCC TTC T-3 ' ) did not induce significant IL-12 secretion even at 50 mg/kg
Koh et al., J Immunol 1997 (Lymphoma, B-Cell) : On the other hand, PECs from tumor treated mice produce IL-12 in response to LPS , suggesting that they are primed in vivo
Probst et al., Eur J Immunol 1997 : IL-12 production did not require CD40 triggering, confirming that the ability of LeIF to induce IL-12 was not mediated through an effect on T cells
McRae et al., Eur J Immunol 1997 : IFN-beta inhibited production of Th2 cytokines ( IL-5 and IL-13 ) and augmented IL-12 mediated IL-10 secretion
Di Renzo et al., Immunology 1997 (Lymphoma, T-Cell, Cutaneous...) : In particular we evaluated in vitro phytohaemagglutinin ( PHA ) -stimulated proliferation and production of interleukin-4 (IL-4) and interferon-gamma (IFN-gamma) as well as lipopolysaccharide (LPS) induced production of IL-12
Sacco et al., Blood 1997 : The IL-12 predose also inhibited IL-12 induced IFN-gamma levels, but did not modify IL-12 induced CS, TNF or sTNFR-P75 ... The IL-12 predose also inhibited IL-12 induced IFN-gamma levels, but did not modify IL-12 induced CS, TNF or sTNFR-P75 ... These data indicate that IL-12 can induce tolerance to its own toxicity, probably through a downregulation of IL-12 induced IFN-gamma but independently of endogenous glucocorticoids
Yang et al., Blood 1997 (Graft vs Host Disease) : This study demonstrates that IL-12 induced IFN-gamma production plays a role in the protective effect of IL-12 against GVHD
Krakowski et al., Eur J Immunol 1997 (Encephalomyelitis, Autoimmune, Experimental) : We have thus shown that a Th1 cytokine bias within the CNS can be induced by CNS APC , and that IL-12 is up-regulated in microglial cells within the CNS of mice with EAE
Berger et al., Eur J Immunol 1997 : IC formed of tetanus toxoid and polyclonal anti-tetanus toxoid antiserum as well as heat aggregated human serum IgG almost completely inhibited IL-12 ( p70 and p40 ) secretion induced by interferon-gamma and lipopolysaccharide in human blood derived monocytes ... Neutralizing anti-IL-10 antibodies plus indomethacin restored IL-12 secretion in the presence of IC to a high extent, indicating that IL-10 and prostaglandin ( PG ) partially mediate the IC-induced inhibition of IL-12 secretion ... We found that exogenously added TNF-alpha caused a profound inhibition of monocytic IL-12 secretion in the absence of IC, again mediated via the induction of IL-10 and PG
Diab et al., Clin Immunol Immunopathol 1997 (Meningitis, Haemophilus) : At this time point, strong expression of IL-6 and TGF-beta was detected in the brain, and also of IL-10 at 48 hr while IFN-gamma and IL-12 were expressed at very low levels throughout the observation time
Rabinovitch et al., Transplantation 1997 : Polymerase chain reaction analysis of cytokine mRNA expression in islet grafts at 8-12 days after transplantation revealed that CsA decreased mRNA levels of type 1 cytokines ( IFN-gamma and IL-12p40 ), whereas CsA plus IL-10 did not, and CsA plus IL-4 increased mRNA levels of IFN-gamma, IL-12p40 , and TGF-beta
Leonard et al., Crit Rev Immunol 1997 (Disease Models, Animal...) : Although IL-12 induced IFN-gamma does not appear to be required for exacerbation of disease, neutralizing antibodies against murine IL-12 delay the onset and reduce the severity of adoptively transferred EAE, indicating a role for endogenous IL-12 as regulator of disease
Melby et al., Infect Immun 1998 (Leishmaniasis, Cutaneous...) : Progressive splenic parasitism was associated with an early IL-4 response, markedly increased IL-10 but minimal IL-12 expression, and delayed expression of iNOS ... Progressive splenic parasitism was associated with an early IL-4 response, markedly increased IL-10 but minimal IL-12 expression, and delayed expression of iNOS
Okano et al., Int Arch Allergy Immunol 1998 (Asthma) : We examined the effects of IL-12 on the productions of IL-5 and granulocyte/macrophage colony stimulating factor ( GM-CSF ) by antigen ( Dermatophagoides farinae, Df ) -stimulated mononuclear cells ( MNCs ) from asthmatic patients in vitro ... We examined the effects of IL-12 on the productions of IL-5 and granulocyte/macrophage colony stimulating factor ( GM-CSF ) by antigen ( Dermatophagoides farinae, Df ) -stimulated mononuclear cells ( MNCs ) from asthmatic patients in vitro ... IL-12 enhanced IFN-gamma production and inhibited the production of IL-5 but not of GM-CSF by Df-stimulated MNCs from asthmatic patients ... IL-12 enhanced IFN-gamma production and inhibited the production of IL-5 but not of GM-CSF by Df-stimulated MNCs from asthmatic patients ... Exogenous IFN-gamma directly inhibited IL-5 production by Df-stimulated MNCs and the inhibition of IL-5 production by IL-12 was partially blocked by anti-IFN-gamma antibody in culture, indicating that inhibitory effect of IL-12 on IL-5 production by antigen stimulated MNCs is partially dependent on IFN-gamma production
Gong et al., J Clin Invest 1998 (HIV Infections) : IFN-gamma production was augmented in a nonantigen-specific manner by IL-12 in 4/8 lines
Kishikawa et al., Toxicol Appl Pharmacol 1997 (Listeriosis) : Support for the former hypothesis is based on the observation that Pb induced elevated levels of p40 splenic messenger RNA since increased p40 expression would result from lack of IL-12 formation
Anitescu et al., J Interferon Cytokine Res 1997 : Additionally, we found that IL-10 inhibited bDNA induced IL-12 secretion by the macrophage cell lines J774 and RAW 264
Gollob et al., Blood 1998 : To understand how the activation of these STATs contributes to T-cell IL-12 responsiveness, we analyzed the IL-12 induced activation of STAT5 and STAT1 in T cells stimulated to undergo Th1 or Th2 differentiation ... To understand how the activation of these STATs contributes to T-cell IL-12 responsiveness, we analyzed the IL-12 induced activation of STAT5 and STAT1 in T cells stimulated to undergo Th1 or Th2 differentiation ... The IL-12 induced tyrosine phosphorylation of STAT5 and STAT1 , but not STAT4, is augmented in T cells activated into Th1 cells with PHA + interferon-gamma (IFN-gamma) compared with T cells activated with PHA alone ... The IL-12 induced tyrosine phosphorylation of STAT5 and STAT1, but not STAT4 , is augmented in T cells activated into Th1 cells with PHA + interferon-gamma (IFN-gamma) compared with T cells activated with PHA alone ... The IL-12 induced tyrosine phosphorylation of STAT5 and STAT1, but not STAT4, is augmented in T cells activated into Th1 cells with PHA + interferon-gamma (IFN-gamma) compared with T cells activated with PHA alone ... STAT5 DNA binding induced by IL-12 is also augmented in T cells activated with PHA + IFN-gamma compared with T cells activated with PHA alone, whereas STAT4 DNA binding is not increased ... The enhancement of IL-12 signaling by IFN-gamma is not a direct effect of IFN-gamma on T cells, but rather is mediated by IL-12 that is produced by antigen presenting cells in response to IFN-gamma ... This positive autoregulatory effect of IL-12 on the activation of select STATs correlates with an increase in T-cell IFN-gamma production in response to IL-12
Osna et al., Scand J Clin Lab Invest 1997 (Hepatitis C, Chronic...) : Recombinant IL-12 ( rIL-12 ) also enhanced IFN gamma of CHC patients, but IL-12 production was decreased in CHC
Myers et al., Am J Pathol 1998 : Although constitutive expression of E- and P-selectin was negligible, Il-12 induced a moderate rise in E-selectin levels
Takeuchi et al., J Immunol 1998 (Hypersensitivity, Delayed) : We have found that : 1 ) TGF-beta2 impaired the capacity of PEC to produce IL-12 and to express CD40 ; 2 ) reduced CD40 expression on TGF-beta2 treated PEC impaired IL-12 production when the cells were cocultured with DO11.10 T cells ; 3 ) the failure of TGF-beta2 treated PEC to stimulate DO11.10 T cells to secrete IFN-gamma was due to their impaired IL-12 production
Bagenstose et al., J Immunol 1998 (Autoimmune Diseases) : This observation may be related to our unexpected finding that IL-12 further potentiated the HgCl2 triggered IL-4 induction in this model
McDyer et al., J Immunol 1998 : The presence of CD40T in cultures increased the production of IL-12 and IFN-gamma from PBMCs stimulated with varying amounts of PHA ... The enhancement of IFN-gamma production induced by direct CD28 stimulation was primarily dependent on endogenous IL-12 or CD40L/CD40 stimulation
Karpus et al., J Exp Med 1998 (Encephalomyelitis, Autoimmune, Experimental) : Increase in MCP-1 expression resulted in downregulation of mucosal interleukin (IL)-12 expression with concomitant increase in mucosal IL-4 expression
Vizler et al., Br J Cancer 1998 (Hemangiosarcoma...) : These results strongly suggest that the anti-tumour effect of IL-12 is principally mediated by IFN-gamma release that in turn blocks H5V cell proliferation and induces the release of factors that suppress angiogenesis
Fantuzzi et al., Blood 1998 : The reduced levels of IFNgamma in ICE-deficient mice were not due to a lack of IL-12 , because zymosan induced IL-12 equally in WT and in ICE-deficient mice
Gri et al., J Biol Chem 1998 (Cell Transformation, Viral) : Transient expression of Ets-2 and c-Rel in B, T, and monocytic cell lines synergistically activated the IL-12 p40 promoter, apparently overcoming the requirement for cell type- or stimulant-specific transcription factors ... Transient expression of Ets-2 and c-Rel in B, T, and monocytic cell lines synergistically activated the IL-12 p40 promoter, apparently overcoming the requirement for cell type- or stimulant-specific transcription factors
DeKruyff et al., J Immunol 1998 : The reduction in IL-12 production by corticosteroid treated macrophages decreased their ability to induce IFN-gamma and increased their ability to induce IL-4 synthesis in Ag-primed CD4+ T cells
Scharton-Kersten et al., J Immunol 1998 (Acute Disease...) : Because resistance of SCID mice to Toxoplasma gondii requires IL-12 dependent IFN-gamma production by NK cells, we expected that gamma(c)-deficient mice would succumb rapidly to the parasite
Mehrotra et al., J Immunol 1998 : However, IL-12 alone induced neither substantial proliferation nor detectable IL-10 production by fresh NK cells, but it synergized with IL-2 in inducing IL-10 mRNA expression and protein synthesis
Liu et al., Cytokine 1998 (Lupus Erythematosus, Systemic) : Exogenous recombinant ( r ) IL-10 strongly inhibited IL-12 production by both SLE and normal PBMC and anti-IL-10 neutralizing antibody significantly reversed the IL-12 deficiency of SLE PBMC and SLE monocytes, while not affecting normal PBMC ... Recombinant interferon gamma ( rIFN-gamma ) considerably enhanced IL-12 production in both SLE and normal PBMC, but it did not significantly reverse the inhibitory effect of rIL-10 on IL-12 production
Liu et al., Cytokine 1998 (Lupus Erythematosus, Systemic) : Our previous studies demonstrated that its production was significantly impaired in systemic lupus erythematosus ( SLE ) patients and this deficient IL-12 production was mainly mediated by excessive endogenous IL-10
Girouard et al., Alcohol Clin Exp Res 1998 : Moreover, acute ethanol treatment augmented IL-12 production in IFN-gamma treated monocytes in response to SEB stimulation ( 25 mM ethanol, p < 0.01 ; 100 mM ethanol, p < 0.01 )
Gasperini et al., AIDS 1998 (HIV Infections) : Furthermore, the release of IL-12 induced by LPS or LPS plus IFNgamma did not significantly differ between PMN from HIV positive patients and healthy donors ... Furthermore, the release of IL-12 induced by LPS or LPS plus IFNgamma did not significantly differ between PMN from HIV positive patients and healthy donors ... Concerning PBMC, the production of TNF-alpha and IL-12 in response to LPS, or LPS plus IFNgamma , was found to be significantly higher in cells isolated from HIV positive patients, whereas the release of IL-1beta was significantly lower ... Concerning PBMC, the production of TNF-alpha and IL-12 in response to LPS , or LPS plus IFNgamma, was found to be significantly higher in cells isolated from HIV positive patients, whereas the release of IL-1beta was significantly lower
Hiramatsu et al., Cancer Immunol Immunother 1998 (Lung Neoplasms...) : Moreover, pleural MNC produced a signficant amount of IFNgamma in response to IL- 12
Chougnet et al., Eur J Immunol 1998 (HIV Infections...) : CD40 ligand and IFN-gamma synergistically restore IL-12 production in HIV infected patients ... CD40 ligand and IFN-gamma synergistically restore IL-12 production in HIV infected patients ... IL-12 production was not altered by CD40L alone, was increased by IFN-gamma , and was synergistically restored to normal values by IFN-gamma + CD40L
Snijders et al., Clin Exp Immunol 1998 (Dermatitis, Atopic) : IL-12 selectively enhances IFN-gamma production and favours the development of IFN-gamma producing Th cells, whereas PGE2 selectively inhibits IFN-gamma production by Th cells
Moriggl et al., J Immunol 1998 : In Th1 cells, IL-12 activated both STAT6 and STAT4 , and IL-4 activated STAT6, but in both cases the Th1 phenotype remained ... In Th1 cells, IL-12 activated both STAT6 and STAT4, and IL-4 activated STAT6 , but in both cases the Th1 phenotype remained ... In Th1 cells, IL-12 activated both STAT6 and STAT4, and IL-4 activated STAT6, but in both cases the Th1 phenotype remained
Ogasawara et al., J Immunol 1998 (Shwartzman Phenomenon) : IFN-gamma induced by IL-12 is a crucial cytokine in the priming phase ... In vivo depletion of both NK cells and NK1+ alphabeta T cells of mice by anti-NK1.1 Ab greatly reduced the elevation of serum IFN-gamma induced by IL-12 and significantly reduced mortality after subsequent injection of LPS, whereas depletion of NK cells alone by anti-asialo GM1 Ab only partially decreased serum IFN-gamma, and lethality was not changed
Yoshimoto et al., J Immunol 1998 (Disease Susceptibility...) : To investigate the antagonistic effect of IL-12 p40 on IL-12 activity in vivo, we generated transgenic ( Tg ) mice in which p40 gene was regulated by a liver-specific promoter
Kawamura et al., J Immunol 1998 : Moreover, while IL-12 induces high levels of IFN-gamma and cytotoxic activity of hepatic or splenic mononuclear cells against tumor cells, this effect of IL-12 is significantly reduced in CD1-deficient mice with impaired NK1+ T cells development ... These results indicate that NK1+ T cells play a critical role in IL-12 induced production of IFN-gamma to initiate Th1 immune responses and as IL-12 induced cytotoxic effector cells to initiate antitumor immunity
Lavigne et al., J Immunol 1998 (Candidiasis) : These results suggested that IFN-gamma induced by IL-12 treatment contributed to lethality
Bohn et al., J Immunol 1998 (Yersinia Infections) : IL-12 induced IFN-gamma production is essential for clearance of Yersinia enterocolitica infection ... Heat killed yersinia triggered IL-18 promoted IFN-gamma production of splenocytes was predominantly dependent on endogenous IL-12 production, whereas IL-12 promoted IFN-gamma production was not IL-18 dependent ... Analysis of the effect of IL-18 on IL-12 production of LPS stimulated peritoneal macrophages revealed that IL-18 decreased LPS induced IL-12 production, indicating that IL-18 might be involved in negative regulation of IL-12 production ... Analysis of the effect of IL-18 on IL-12 production of LPS stimulated peritoneal macrophages revealed that IL-18 decreased LPS induced IL-12 production, indicating that IL-18 might be involved in negative regulation of IL-12 production
Morein et al., Dev Biol Stand 1998 : Further, iscoms induce APC to produce IL-1, IL-6 and IL-12 and a TH1 type of response with enhanced IL-2 and IFN-gamma production
Barbulescu et al., J Immunol 1998 : IL-12 and IL-18 differentially regulate the transcriptional activity of the human IFN-gamma promoter in primary CD4+ T lymphocytes ... We analyzed the molecular mechanisms by which IL-12 and IL-18 induce transcriptional activity of the IFN-gamma promoter in primary human CD4+ T cells ... These data suggest that both AP-1 and STAT4 are required for IL-12 dependent IFN-gamma promoter activity, whereas IL-18 causes direct activation via AP-1
Tomura et al., J Immunol 1998 : After stimulation with anti-CD3 plus anti-CD28 mAbs, CD8+, CD4+, and CD4-CD8- T cells expressed IL-12R, but not IL-18R, and produced IFN-gamma in response to IL-12 ... These results indicate that the induction of IL-18R and IL-18 responsiveness by IL-12 represents a mechanism underlying enhanced IFN-gamma production by resting T cells, but the operation of this mechanism differs depending on the T cell subset stimulated
Vingerhoets et al., Immunol Lett 1998 (HIV Infections) : IL-10 was only induced upon stimulation with IL-2 and IL-12 and tended to be produced more in patients
Belkaid et al., Eur J Immunol 1998 (Inflammation...) : Infection of inflammatory macrophages in vitro using metacyclic promastigotes produced identical effects on cytokine responses regardless of whether cells from genetically resistant or susceptible mouse strains were used : IL-12 was not produced in response to infection itself, virtually every infected cell lost its ability to produce IL-12 in response to IFN-gamma/LPS , and the IL-6 response was partially inhibited, while the TNF-alpha response of infected cells was unimpaired ... Infection of inflammatory macrophages in vitro using metacyclic promastigotes produced identical effects on cytokine responses regardless of whether cells from genetically resistant or susceptible mouse strains were used : IL-12 was not produced in response to infection itself, virtually every infected cell lost its ability to produce IL-12 in response to IFN-gamma/LPS, and the IL-6 response was partially inhibited , while the TNF-alpha response of infected cells was unimpaired ... Infection of inflammatory macrophages in vitro using metacyclic promastigotes produced identical effects on cytokine responses regardless of whether cells from genetically resistant or susceptible mouse strains were used : IL-12 was not produced in response to infection itself, virtually every infected cell lost its ability to produce IL-12 in response to IFN-gamma/LPS , and the IL-6 response was partially inhibited, while the TNF-alpha response of infected cells was unimpaired
Sweet et al., J Interferon Cytokine Res 1998 : Bacterial DNA has been shown to induce IFN-gamma production in vivo as an indirect consequence of interleukin-12 (IL-12) and TNF-alpha production from macrophages
Tsung et al., J Immunol 1998 (Disease Models, Animal...) : IL-12 is apparently the only cytokine capable of mediating tumor eradication, and the effect is dependent on IFN-gamma
Bromuro et al., Infect Immun 1998 (Candidiasis) : Inoculation of CaHsp70 preparations into immunized mice induced rapid production of interleukin-6 (IL-6) and tumor necrosis factor alpha, peaking at 2 to 5 h and declining within 24 h. CaHsp70 and CaHsp70-Cter also induced gamma interferon ( IFN-gamma ), IL-12 , and IL-10 but not IL-4 production by CD4+ lymphocytes cocultured with splenic accessory cells from nonimmunized mice
McRae et al., J Immunol 1998 : Suppression of p40 IL-12 and IFN-gamma was not due to increased levels of IL-10 in these cultures, and production of IFN-gamma could be restored by exogenous IL-12 ... Suppression of p40 IL-12 and IFN-gamma was not due to increased levels of IL-10 in these cultures, and production of IFN-gamma could be restored by exogenous IL-12
García et al., J Immunol 1998 : Moreover, we showed that this increase in IFN-gamma could be explained by the dual activation of STAT1 and STAT4 by IL-15 and IL-12 , respectively ... Moreover, we showed that this increase in IFN-gamma could be explained by the dual activation of STAT1 and STAT4 by IL-15 and IL-12 , respectively
Matsumoto et al., Nephron 1998 (Glomerulonephritis, IGA...) : Prior studies have shown that interleukin (IL) 12 promotes T helper type 1 differentiation and enhances production of T helper type 1 cytokines such as gamma interferon and IL-2 ... We, therefore, investigated the effects of recombinant human IL-12 on the release of VPF by peripheral blood mononuclear cells ( PBMC ) from LN patients ... Recombinant human IL-12 was found to enhance VPF release in a dose dependent manner
Cordero et al., Immunol Lett 1998 : Recently, we reported that IL-12 increased expression and function of CD26/DPPIV , this may be a new cellular pathway of the Th1-like immune responses ... Here, we looked for a specific subset which would respond to CD26 upregulation by IL-12 ... By using dual fluorescence analysis, IL-12 dependent CD26 expression was found in both CD4 and CD8 ( previously CD26+ or CD26- ) activated T cells and, moreover, the CD45RO percentage was unaffected
Braley-Mullen et al., Am J Pathol 1998 (Granuloma...) : Induction of severe granulomatous EAT by IL-12 required MTg in vitro and was unrelated to the IL-12 induced increase in IFN-gamma production ... Induction of severe granulomatous EAT by IL-12 required MTg in vitro and was unrelated to the IL-12 induced increase in IFN-gamma production ... IL-12 markedly increased IFN-gamma production but did not induce a shift to a Th1-dominant phenotype, as other Th1 and Th2 cytokines were generally unaffected and both Th1 and Th2 cytokines were expressed in recipient thyroids
Yeaman et al., J Immunol 1998 : We further found that polymorphonuclear neutrophils isolated from normal donors produce IFN-gamma in response to stimulation with LPS, IL-12 , and TNF-alpha
Allison et al., Arch Immunol Ther Exp (Warsz) 1997 : Adjuvants also up-regulate a secondary cascade of cytokines in lymphoid tissues responding to antigenic stimulation : IL-12 augments the production of IFN-gamma , which favors the production of antibodies of protective isotypes ( IgG2a in the mouse )
Contreras et al., Transplantation 1998 : DSG was shown to inhibit IL-12-driven IFN-gamma production by a mechanism associated with inhibition of nuclear factor kappa-B
Leite-De-Moraes et al., Eur J Immunol 1998 : A similar effect of IL-12 on IFN-gamma production was observed when NK T lymphocytes were obtained after expansion with IL-7 ... It is noteworthy that whatever cytokines are used for their expansion, IL-12 stimulation, in the absence of TCR/CD3 cross linking, promotes consistent IFN-gamma secretion by NK T cells without detectable IL-4 production
D'Orazio et al., Eur J Immunol 1998 : In addition, the cytokine profiles of ocular APC pulsed with OVA or OVA-latex showed that soluble OVA induced the production of IL-10 , whereas OVA-latex induced the production of IL-12 ... Moreover, they suggest a mechanism in which soluble antigens preferentially induce the release of ACAID inducing IL-10 whereas particulate antigens preferentially induce the release of Th1 inducing IL-12 by responding APC
Poggi et al., Eur J Immunol 1998 : IL-12 induced up-regulation of NKRP1A expression in human NK cells and consequent NKRP1A mediated down-regulation of NK cell activation ... IL-12 , in contrast to IL-2, strongly up-regulated the expression of the NKRP1A lectin molecule on human NK cells ... The IL-12 induced NKRP1A expression was time and dose dependent, reaching a maximum by 7 days of culture in the presence of 2 ng/ml IL-12 and it was inhibited by the addition of anti-IL-12 monoclonal antibody ... The IL-12 dependent NKRP1A up-regulation was abrogated by the incubation of NK cells with actinomycin D, thus suggesting that IL-12 induces de novo transcription of NKRP1A mRNA
Altare et al., Science 1998 (Granuloma...) : Thus, IL-12 dependent IFN-gamma secretion in humans seems essential in the control of mycobacterial infections, despite the formation of mature granulomas due to IL-12 independent IFN-gamma secretion
Büttner et al., Cytokine 1998 : Supernatants of canine PBMC stimulated for IL-12 release also induced interferon gamma (IFN-gamma) mRNA as detectable by RT-PCR ; however, it was not clear whether IFN-gamma mRNA synthesis was due to an IL-12 specific effect or other stimuli ... As to the stimulating effect of IL-12 on canine IFN-gamma mRNA synthesis, recombinant human IL-12 was found to be a good inducer
Petrovsky et al., Cytokine 1998 : To investigate the latter, the authors performed measurements of lipopolysaccharide (LPS) stimulated whole blood, interferon gamma (IFN-gamma), tumour necrosis factor alpha (TNF-alpha), interleukin 1 (IL-1) and IL-12 production in 13 healthy volunteers over 24 h
Benyoucef et al., J Med Virol 1998 (Disease Progression...) : The results indicate a decreased production of type 1 cytokines ( IL12 , IFN gamma ) in the presence of a relatively preserved production of type 2 cytokines ( IL4 , IL10 ) in HIV-1 infected patients
Munder et al., J Exp Med 1998 : Murine macrophages secrete interferon gamma upon combined stimulation with interleukin (IL)-12 and IL-18 : A novel pathway of autocrine macrophage activation ... Although interleukin (IL)-12 and IL-18 alone induce low levels of IFN-gamma mRNA transcripts, the combined stimulation of BMMPhi with both cytokines leads to the efficient production of IFN-gamma protein
Heinzel et al., Cell Immunol 1998 (Leishmaniasis, Cutaneous) : We conclude that CD40 dependent responses are continually required for healing of leishmaniasis and that progressive disease is associated with decreased CD40 stimulated IL-12 synthesis as a consequence of either altered cytokine environment or inadequate accessory cell number
Okamura et al., Curr Opin Immunol 1998 : Regulation of interferon-gamma production by IL-12 and IL-18
Lee et al., Leukemia & lymphoma 1998 : Our laboratory has investigated the regulation of IL-12 gene expression in both cord blood and adult PBMC, and the effects of IL-12 on induction of IFN-gamma production, NK, and lymphokine activated killer ( LAK ) cytotoxicity ... Exogenous IL-12 induced a significant increase of IFN-gamma from both cord and adult PBMC
Ria et al., Eur J Immunol 1998 : Among Th2 derived cytokines, IL-10 , but not IL-4 inhibit Th1-driven IL-12 secretion ... Among Th2 derived cytokines, IL-10, but not IL-4 inhibit Th1-driven IL-12 secretion
Lauwerys et al., Eur J Immunol 1998 (Chronic Disease...) : Although IL-12 down-regulated IL-4, IL-5, IL-9 and IL-10 production, its inhibitory activity on Ig production could not be ascribed to down-regulation of these cytokines, as addition of saturating doses of IL-4, IL-5 and/or IL-9 did not reverse the inhibitory activity of IL-12 ... Although IL-12 down-regulated IL-4 , IL-5, IL-9 and IL-10 production, its inhibitory activity on Ig production could not be ascribed to down-regulation of these cytokines, as addition of saturating doses of IL-4, IL-5 and/or IL-9 did not reverse the inhibitory activity of IL-12 ... Although IL-12 down-regulated IL-4, IL-5 , IL-9 and IL-10 production, its inhibitory activity on Ig production could not be ascribed to down-regulation of these cytokines, as addition of saturating doses of IL-4, IL-5 and/or IL-9 did not reverse the inhibitory activity of IL-12 ... Although IL-12 down-regulated IL-4, IL-5, IL-9 and IL-10 production, its inhibitory activity on Ig production could not be ascribed to down-regulation of these cytokines, as addition of saturating doses of IL-4, IL-5 and/or IL-9 did not reverse the inhibitory activity of IL-12
Frey et al., J Immunol 1998 : NK activation by PMA, IL-2, IL-15, or TGF-beta down-regulated L-selectin on the CD56bright subset, while increased L-selectin levels were observed in both the CD56bright and CD56dim NK subsets in response to IL-12 , IL-10, or IFN-alpha
Grewe et al., J Immunol 1998 : The expression of both IL-12 protein and mRNAs for the p35 and p40 IL-12 subunits was strongly induced in all donors by the Th2-like cytokines IL-4 and granulocyte-macrophage CSF and was also moderately induced by TNF-alpha and IL-1alpha ... The expression of both IL-12 protein and mRNAs for the p35 and p40 IL-12 subunits was strongly induced in all donors by the Th2-like cytokines IL-4 and granulocyte-macrophage CSF and was also moderately induced by TNF-alpha and IL-1alpha ... The expression of both IL-12 protein and mRNAs for the p35 and p40 IL-12 subunits was strongly induced in all donors by the Th2-like cytokines IL-4 and granulocyte-macrophage CSF and was also moderately induced by TNF-alpha and IL-1alpha ... IL-5 treatment resulted in IL-12 synthesis in four atopic donors and one nonatopic donor, whereas IFN-gamma induced IL-12 synthesis in only two atopic donors
Sutterwala et al., J Exp Med 1998 (Inflammation) : In this report, we demonstrate that ligation of macrophage Fcgamma receptors ( FcgammaR ) can lead to a reversal of macrophage proinflammatory responses by inducing an upregulation of interleukin (IL)-10 , with a reciprocal inhibition of IL-12 production ... In all of the models tested, the ligation of FcgammaR promoted the production of IL-10 and inhibited the secretion of IL-12
Okuno et al., J Surg Res 1998 (Adenocarcinoma...) : In addition, we examined whether activation of HSL by interleukin-12 (IL-12) can prevent liver metastasis of highly metastatic clone RCN-H4
Collins et al., Vet Immunol Immunopathol 1998 : The presence of IL-12 markedly influenced the level of IFNg secreted by these cells, and although IL-12 induced IFNg production in the absence of antigenic stimulation, IFNg production was accelerated and augmented in response to IL-12 and antigen
Yahata et al., Immunol Lett 1998 (Lymphatic Diseases...) : It was also confirmed that IL-12 activated IFN-gamma producing Th1 cells were accumulated in tumor local site
Pyeon et al., J Virol 1998 (Enzootic Bovine Leukosis...) : The present study combined with previous findings suggest that IL-12 in bovine leukemia virus infected animals may regulate production of other cytokines such as gamma interferon and IL-10 and the progression of bovine leukosis in animals that develop more advanced disease such as a persistent lymphocytosis of B cells or B-cell lymphosarcoma
Du et al., J Leukoc Biol 1998 : Mechanism of inhibition of LPS induced IL-12p40 production by IL-10 and TGF-beta in ANA-1 cells
Newton et al., Adv Exp Med Biol 1998 : It was found that THC suppressed IL-12 , IL-15 and IL-6 and increased IL-1 alpha, IL-1 beta, and TNF alpha in all of the stimulated cultures
Lissoni et al., Br J Cancer 1998 (Carcinoma, Renal Cell...) : This study was performed to evaluate the effects of subcutaneous ( s.c. ) low-dose IL-2 on IL-12 secretion in metastatic cancer patients
Németh et al., Brain Res Bull 1998 (Endotoxemia) : Intraperitoneal ( i.p. ) pretreatment with dantrolene ( 20 mg/kg ), but not verapamil ( 10 mg/kg, i.p. ) or diltiazem ( 20 mg/kg, i.p. ) suppressed the LPS induced ( 80 mg/kg, i.p. ) plasma levels of IL-12 and IFN-gamma in BALB/c mice
Flynn et al., J Exp Med 1998 : Furthermore, T cell stimulation through OX40 inhibits IFN-gamma expression in both CD8 T cells and IL-12 stimulated CD4 T cells ... Furthermore, T cell stimulation through OX40 inhibits IFN-gamma expression in both CD8 T cells and IL-12 stimulated CD4 T cells
Ito et al., AIDS Res Hum Retroviruses 1998 : We examined the possibility that Tat suppresses IL-12 production by PBMCs from healthy donors ... Tat significantly inhibited IL-12 production by human PBMCs stimulated with Staphylococcus aureus Cowan 1 strain ( SAC ) at concentrations between 5 and 40 ng/ml. Immunoabsorption by using polyclonal antibody to Tat abolished the suppression of the IL-12 production by Tat ... IL-12 production by monocytes, separated from PBMCs by the adhesion method, was also inhibited by Tat
Constantinescu et al., Immunol Lett 1998 : We show that both ACE inhibitors significantly inhibit production of IL-12 by PBMC stimulated with bacterial lipopolysaccharide (LPS) or Staphylococcus aureus Cowan ( SAC )
Jones et al., Infect Immun 1998 (Disease Models, Animal...) : Neutralizing IL-4 and the administration of exogenous IL-12 upregulate IL-12R expression in BALB/c mice, while the neutralization of IL-12 in C3H mice blocks increased IL-12 receptor expression
Kelleher et al., Int Immunol 1998 : IL-12 increases CD80 expression and the stimulatory capacity of bone marrow derived dendritic cells
Harada et al., Cancer Res 1998 (Melanoma, Experimental) : The in vivo depletion of either CD4+ or CD8+ T cells abrogated the antitumor activity of IL-12 and diminished the apparent autocrine stimulation of IL-12 release seen after IL-12 treatment
Silva et al., Braz J Med Biol Res 1998 (Disease Models, Animal...) : On the other hand, TGF-beta and IL-10 produced by infected macrophages inhibited the induction and effects of IL-12
Ribeiro-de-Jesus et al., Braz J Med Biol Res 1998 (Leishmaniasis) : Moreover, IL-12 restores IFN-gamma production and enhances the cytotoxic response
Bussolati et al., J Immunol 1998 : The results obtained indicate that IL-12 induces a dose- and time dependent synthesis of platelet activating factor (PAF) from PMN and NK cells and of reactive oxygen radicals ( ROS ) from PMN ... In conclusion, the results of the present study indicate that IL-12 induced PAF synthesis plays a critical role in triggering the events involved in the motogenic response of PMN and NK to IL-12
Constantinescu et al., Eur J Immunol 1998 (Leishmaniasis) : However, when a short-term T cell line was established in vitro from lymph node cells, the production of IFN-gamma again became IL-12 dependent
Coughlin et al., Immunity 1998 (Mammary Neoplasms, Experimental...) : IL-12 induced angiogenesis inhibitory activity in the responsive cells, which may be attributable to production of the chemokine IP-10
Weinheber et al., Eur J Immunol 1998 (Leishmaniasis, Cutaneous) : IL-12 enhances IFN-gamma production by Th1 cells and is crucial for cure ... Suppression is independent of engagement of CR3 or FcgammaR during phagocytosis, is not mediated by IL-10 and does not alter steady state IL-12p40 mRNA levels
Suen et al., Vaccine 1998 : Furthermore, we have studied the effects of IL-12 and IL-15 on induction of IFN-gamma and TNF-alpha production, NK, and LAK activities in CB and APB ... Furthermore, we have studied the effects of IL-12 and IL-15 on induction of IFN-gamma and TNF-alpha production, NK, and LAK activities in CB and APB ... The impaired ability of CB to produce IL-12 and IL-15 in response to stimulation may contribute to the decrease in IFN-gamma, TNF-alpha production, NK and LAK activities ... The impaired ability of CB to produce IL-12 and IL-15 in response to stimulation may contribute to the decrease in IFN-gamma , TNF-alpha production, NK and LAK activities
Bright et al., J Immunol 1998 : TGF-beta inhibits IL-12 induced activation of Jak-STAT pathway in T lymphocytes ... TGF-beta inhibits IL-12 induced activation of Jak-STAT pathway in T lymphocytes
Sternesjö et al., J Endocrinol 1998 : IL-2 + IL-12 addition induced a small decrease in glucose stimulated insulin release in islets from 12-week-old animals
Liu et al., Int Immunol 1998 : Furthermore, the ability of IL-10 DC to prime for IL-4 secreting T cells was closely related to the down-regulation of CD40 ligand mediated IL-12 p70 production by DC in the primary cultures and was markedly reduced by adding exogenous IL-12 to the priming cultures
Wijesuriya et al., Int Immunol 1998 (Neoplasms, Experimental) : IFN-gamma-selective up-regulation was due to an enhancement of IL-12 production because IL-12 was detected in B cell depleted cultures and enhanced IFN-gamma production was prevented by addition of anti-IL-12 mAb or anti-CD40 ligand (CD40L) mAb capable of inhibiting CD40L induced IL-12 production ... IFN-gamma-selective up-regulation was due to an enhancement of IL-12 production because IL-12 was detected in B cell depleted cultures and enhanced IFN-gamma production was prevented by addition of anti-IL-12 mAb or anti-CD40 ligand (CD40L) mAb capable of inhibiting CD40L induced IL-12 production ... These results indicate that B cells interfere with IFN-gamma production induced through interactions between anti-tumor T cells and APC, and this suppressive effect is based on the capacity of CD40+ B cells to down-regulate the CD40L induced IL-12 production by APC
Xin et al., J Neuroimmunol 1998 : In this study, we investigated the effect of VIP on IL-12 , TNF alpha and nitric oxide ( NO ) production in macrophages following activation with lipopolysaccharide (LPS) or superantigens ... In vitro studies show that at physiologic concentrations, VIP inhibited IL-12 and NO but not TNF alpha production in macrophages which were stimulated with LPS or superantigens ... The inhibitory effect of VIP on IL-12 production appeared to be cAMP mediated since other cAMP inducing agents were also potent in inhibiting IL-12 production
Kaliński et al., J Immunol 1998 : The ability of DC to produce IL-12 is also suppressed by IL-10 , which in contrast to PGE2, inhibits their maturation
Assenmacher et al., J Immunol 1998 : Commitment of individual Th1-like lymphocytes to expression of IFN-gamma versus IL-4 and IL-10 : selective induction of IL-10 by sequential stimulation of naive Th cells with IL-12 and IL-4
Davidson et al., J Immunol 1998 (Chronic Disease...) : Because IL-12 can promote Th1 development and IFN-gamma production, the ability of neutralizing anti-IL-12 mAb to modulate colitis in IL-10 ( -/- ) mice was investigated
Schlaak et al., J Med Virol 1998 (Hepatitis C) : Lower baseline IL-12- and LPS induced IFN gamma, TNF alpha, and IL-12 production was observed in group 2 due to a possible dysfunction of accessory cells
Lawrence et al., Infect Immun 1998 : The ability of most bacterial PGs to induce IL-12 production suggests that they play an important role in triggering host defense mechanisms against bacterial infections
Méndez-Samperio et al., Cell Immunol 1998 : We also observed that the ability of BCG to stimulate release of IL-12 from human cells was significantly inhibited by IL-10 ... The inhibitory effect of IL-10 on the secretion of IL-12 was specific, as it was significantly abolished in the presence of anti-IL-10 neutralizing monoclonal antibody ... These results were further confirmed as anti-IL-10 antibodies markedly increased the levels of IL-12, suggesting that BCG induced IL-10 , as well as exogenous IL-10, can regulate IL-12 production by human cells stimulated with M. bovis BCG
Avice et al., J Immunol 1998 : IL-15 promotes IL-12 production by human monocytes via T cell dependent contact and may contribute to IL-12 mediated IFN-gamma secretion by CD4+ T cells in the absence of TCR ligation ... IL-15 promotes IL-12 production by human monocytes via T cell dependent contact and may contribute to IL-12 mediated IFN-gamma secretion by CD4+ T cells in the absence of TCR ligation ... IFN-gamma production requires endogenous IL-12 , the production of which in turn is dependent upon CD40/CD154 interactions between CD4+ T cells and monocytes
Azzoni et al., J Immunol 1998 : Differential transcriptional regulation of CD161 and a novel gene, 197/15a, by IL-2, IL-15, and IL-12 in NK and T cells ... We previously demonstrated that expression of transcription factors ( e.g., Egr-1 , JunB, and c-Fos ) is differentially regulated by IL-2 and IL-12 ... We previously demonstrated that expression of transcription factors ( e.g., Egr-1, JunB, and c-Fos ) is differentially regulated by IL-2 and IL-12 ... We previously demonstrated that expression of transcription factors ( e.g., Egr-1, JunB , and c-Fos ) is differentially regulated by IL-2 and IL-12 ... CD161 transcription, mRNA accumulation, and surface expression are increased by IL-12
Cenci et al., J Immunol 1998 (Candidiasis...) : IFN-gamma is required for IL-12 responsiveness in mice with Candida albicans infection
Dias et al., Int J Cancer 1998 (Mammary Neoplasms, Experimental...) : IL-12 regulates VEGF and MMPs in a murine breast cancer model ... IFN-gamma reduced tumor cell production of VEGF over a 24-hr period in vitro, suggesting that IL-12 induced IFN-gamma may be responsible for the decline in VEGF levels in vivo
Grohmann et al., Immunity 1998 : The unique properties of the IL-12 receptor on DC, characterized by a single class of binding sites with a Kd of about 325 pM, may underlie rather unique effects, such as IFNgamma independent augmentation of class II antigen expression and priming for LPS induced production of IL-12
Zhong et al., Zhongguo Yao Li Xue Bao 1996 : Met-enk promoted NK activity at 1 x 10 ( -8 ) - 1 x 10 ( -5 ) mol.L-1, increased TNF-alpha production both in vitro and in vivo, and enhanced IL-12 p35 gene transcription after ip 0.1 mg.kg-1 for 6 d
Sakurai et al., Int J Immunopharmacol 1998 : NK 1.1+ cells purified from rhM-CSF injected mouse spleen cells exhibited ( a ) higher cytotoxic activity against Yac-1 cells, ( b ) higher proliferative responsiveness to interleukin (IL)-2 and ( c ) a greater production of interferon (IFN)-gamma in response to IL-2 and IL-12 compared to cells purified from vehicle injected mouse spleen cells in vitro
Redford et al., J Immunol 1998 : IL-10 has anti-inflammatory effects and can reduce the production of IL-12 ... To determine the possible role of CsA modulated IL-10 production in mediating the increased IL-12 levels, splenocytes from IL-10 gene disrupted mice (IL-10 -/-) and splenocytes cultured in anti-IL-10 Ab were studied
Shibata et al., J Immunol 1998 : Exogenous IL-10 also inhibited IL-12 and TNF-alpha production by chitin stimulated Mphi
Jiang et al., J Interferon Cytokine Res 1998 : Differential induction of IL-12 by IFN-beta and IFN-gamma in human macrophages ... Differential induction of IL-12 by IFN-beta and IFN-gamma in human macrophages ... IL-12 induces interferon-gamma (IFN-gamma) production and enhances cellular immune responses ... Conversely, IFN-gamma does not induce IL-12 but can prime its production by phagocytic cells in response to antigenic stimuli ... In this study, we examined the effect of IFN-beta on IL-12 production in human macrophages, as IFN-beta is a natural protein produced by virus infected cells ... However, IFN-gamma can enhance IFN-beta induced IL-12 in these cells ... However, IFN-gamma can enhance IFN-beta induced IL-12 in these cells
von Stebut et al., J Exp Med 1998 (Leishmaniasis, Cutaneous) : Parasite internalization was associated with activation manifested by upregulation of major histocompatibility complex ( MHC ) class I and II surface antigens, increased expression of costimulatory molecules ( CD40, CD54, CD80, and CD86 ), and interleukin (IL)-12 p40 release within 18 h. L. major induced IL-12 p70 release by DC required interferon gamma and prolonged ( 72 h ) incubation ... Parasite internalization was associated with activation manifested by upregulation of major histocompatibility complex ( MHC ) class I and II surface antigens, increased expression of costimulatory molecules ( CD40, CD54, CD80, and CD86 ), and interleukin (IL)-12 p40 release within 18 h. L. major induced IL-12 p70 release by DC required interferon gamma and prolonged ( 72 h ) incubation
Lin et al., J Clin Immunol 1998 (HIV Infections) : Effect of interleukin (IL)-12 and IL-15 on activated natural killer (ANK) and antibody dependent cellular cytotoxicity ( ADCC ) in HIV infection
Gurunathan et al., J Immunol 1998 (Colonic Neoplasms...) : Moreover, in a mouse model requiring IL-12 dependent production of IFN-gamma , mice vaccinated with soluble Leishmania Ag and CD40LT DNA were able to control infection with Leishmania major
Constantinescu et al., J Immunol 1998 (Autoimmune Diseases...) : Here, we show that SEA and SEB induce IL-12 in splenocytes from ( PL/J x SJL/J ) F1 mice in vitro and increase the level of IL-12 in the sera of mice treated with these superantigens ... Here, we show that SEA and SEB induce IL-12 in splenocytes from ( PL/J x SJL/J ) F1 mice in vitro and increase the level of IL-12 in the sera of mice treated with these superantigens
Higuchi et al., Kekkaku 1998 : Although production levels of IL-12 from PBMC stimulated with M. tuberculosis were below the detectable level, addition of interferon-gamma (IFN-gamma) or neutralizing antibody against IL-10 augmented the production of IL-12 from PBMC, suggesting that IFN-gamma and IL-10 regulate the production of IL-12 from M phi positively and negatively, respectively ... Although production levels of IL-12 from PBMC stimulated with M. tuberculosis were below the detectable level, addition of interferon-gamma (IFN-gamma) or neutralizing antibody against IL-10 augmented the production of IL-12 from PBMC, suggesting that IFN-gamma and IL-10 regulate the production of IL-12 from M phi positively and negatively, respectively
Uno et al., Clin Cancer Res 1998 (Neoplasms) : Whereas enhanced tumor necrosis factor alpha production was also observed in some patients after IL-12 stimulation, the elevation of tumor necrosis factor alpha was induced only in blood samples that showed IL-12 stimulated IFN-gamma production
Holland et al., J Infect Dis 1998 : These patients demonstrate the critical role that the IFN-gamma receptor plays in the regulation of IFN-gamma, IL-12 , and TNF-alpha
Fulton et al., J Infect Dis 1998 : Regulation of interleukin (IL)-12 production by coexpression of tumor necrosis factor (TNF)-alpha, IL-10, and transforming growth factor ( TGF ) -beta in human monocytes infected with Mycobacterium tuberculosis H37Ra was analyzed ... Regulation of interleukin (IL)-12 production by coexpression of tumor necrosis factor (TNF)-alpha , IL-10, and transforming growth factor ( TGF ) -beta in human monocytes infected with Mycobacterium tuberculosis H37Ra was analyzed ... Regulation of interleukin (IL)-12 production by coexpression of tumor necrosis factor (TNF)-alpha, IL-10 , and transforming growth factor ( TGF ) -beta in human monocytes infected with Mycobacterium tuberculosis H37Ra was analyzed ... Also, since IL-12 induces interferon (IFN)-gamma , the effect of IFN-gamma on IL-12 expression was examined ... TNF-alpha protein expression occurred earlier than IL-12 p40 protein but was not required for IL-12 induction ... In contrast, recombinant IL-10 reduced IL-12 and neutralization of IL-10 minimally enhanced IL-12
Mazzeo et al., Eur J Immunol 1998 : Decreased IL-12 production and Th1 cell development by acetyl salicylic acid mediated inhibition of NF-kappaB ... ASA was found to inhibit secretion of the IL-12 heterodimer as well as p40 monomer by human monocytic cells ... These results suggest that the inhibition of NF-kappaB activation by ASA leads to down-regulation of IL-12 production and inhibition of Th1 cell development
Galli et al., Eur J Immunol 1998 : Similarly, in naive CD4+ T cells activated in the presence of IL-4 or IL-12 and infected with the same T-tropic strain, IL-4 up-regulated whereas IL-12 down-regulated both CXCR4 and HIV-1 expression ... The down-regulatory effect of IL-12 on CXCR4 expression was found to be dependent on its capacity to induce IFN-gamma production
Byl et al., J Interferon Cytokine Res 1998 (Acquired Immunodeficiency Syndrome) : We found that ( 1 ) OM85-BV stimulates IFN-gamma secretion by PBMC from normal individuals and human immunodeficiency virus ( HIV ) -infected patients, ( 2 ) CD4+ cells represent the major source of IFN-gamma produced in response to OM85-BV, and ( 3 ) this effect of OM85-BV involves the induction of interleukin-12 (IL-12) secretion by accessory cells ... We conclude that bacterial extracts might enhance antimicrobial defenses by eliciting IL-12 dependent IFN-gamma synthesis by CD4+ T cells
Hodge-Dufour et al., Proc Natl Acad Sci U S A 1998 (Inflammation) : Interleukin (IL) 12 produced by macrophages in response to various stimuli is a potent inducer of interferon (IFN) gamma production ... IFN-gamma , in turn, markedly enhances IL-12 production ... Inhibition of IL-12 by TNF can be mediated by both IL-10 dependent and IL-10 independent mechanisms ... To determine whether TNF inhibition of IFN-gamma induced IL-12 production contributed to the resolution of an inflammatory response in vivo, the response of TNF+/+ and TNF-/- mice injected with Corynebacterium parvum were compared ... To determine whether TNF inhibition of IFN-gamma induced IL-12 production contributed to the resolution of an inflammatory response in vivo, the response of TNF+/+ and TNF-/- mice injected with Corynebacterium parvum were compared ... IFN-gamma inhibition of chemokine production and inhibition of IFN-gamma induced IL-12 production by TNF provide potential mechanisms by which these cytokines can exert anti-inflammatory/repair function ( s )
Fujimiya et al., Clin Cancer Res 1997 (Brain Neoplasms...) : Thus, the increased expression of the IL-2Rbeta is critical for the synergistic activation of gammadelta T cells by IL-12 plus IL-2 ; it is also probable that at least the low-affinity IL-12R contributes to the activation of gammadelta T cells mediated by either IL-12 alone or IL-12 plus IL-2 ... Thus, the increased expression of the IL-2Rbeta is critical for the synergistic activation of gammadelta T cells by IL-12 plus IL-2 ; it is also probable that at least the low-affinity IL-12R contributes to the activation of gammadelta T cells mediated by either IL-12 alone or IL-12 plus IL-2
Yasuda et al., J Clin Invest 1998 (Diabetes Mellitus, Type 1) : Reverse transcription-PCR analysis revealed that local production of IL-12p40 led to the decrease of interferon-gamma and the augmentation of transforming growth factor-beta at the graft site ... Reverse transcription-PCR analysis revealed that local production of IL-12p40 led to the decrease of interferon-gamma and the augmentation of transforming growth factor-beta at the graft site
van der Pouw Kraan et al., J Clin Invest 1998 : The inhibition of IL-12 production is independent of IL-10 and IFN-gamma ... The inhibition of IL-12 production is independent of IL-10 and IFN-gamma
Toossi et al., J Clin Lab Immunol 1997 (Tuberculosis, Pulmonary) : However, in mononuclear cells from patients with tuberculosis the main effect of TGF-beta on IL-12 appeared to be counter action to IL-12 induced IFN gamma production in response to M. tuberculosis
Vieira et al., J Immunol 1998 : However, as a result of their defective production of bioactive IL-12, GC-pretreated DC have a reduced ability to promote the production of IFN-gamma in CD4+ Th lymphocytes, as shown by the observation that IFN-gamma production could be restored by exogenous IL-12
Takata et al., Clin Exp Immunol 1998 (Mucocutaneous Lymph Node Syndrome) : PHIG greatly reduced the production of IL-12, interferon-gamma (IFN-gamma) and other cytokines from SPE-A stimulated PBMC, while exogenous IL-12 , but neither IL-1 nor tumour necrosis factor-alpha (TNF-alpha), restored IFN-gamma production inhibited by PHIG
Monteleone et al., Gut 1998 (Colitis...) : IFN-gamma mRNA was induced in T-LPLs by IL-12 and IL-15 but not IL-7, whereas IFN-gamma was measured only in IL-12 stimulated T-LPL cultures ... In preactivated T-LPLs, IL-7 enhanced IFN-gamma release induced by both IL-12 and anti-CD2, whereas IL-15 potentiated only IL-12 induced IFN-gamma synthesis ... IL-12 did not induce proliferation of either unstimulated or preactivated T-LPLs and it did not enhance the CD2/CD28 stimulated T-LPL proliferative response ... IL-12 did not induce proliferation of either unstimulated or preactivated T-LPLs and it did not enhance the CD2/CD28 stimulated T-LPL proliferative response
Suemoto et al., J Dermatol Sci 1998 : The expression of MHC class II, CD80 and CD86 on EC was not affected by IL-12 ... The expression of MHC class II, CD80 and CD86 on EC was not affected by IL-12 ... The expression of MHC class II , CD80 and CD86 on EC was not affected by IL-12
Ahn et al., J Immunol 1998 : 2D6 ( IL-12 ) and 2D6 ( IL-2 ) similarly proliferating in response to IL-12 induced comparable levels of JAK2 activation and STAT5 phosphorylation ... 2D6 ( IL-12 ) and 2D6 ( IL-2 ) similarly proliferating in response to IL-12 induced comparable levels of JAK2 activation and STAT5 phosphorylation ... JAK2 was associated with STAT5, and IL-12 induced STAT5 phosphorylation was elicited in the absence of JAK3 activation
Müller et al., Springer Semin Immunopathol 1998 (Arthritis...) : Guided by the principles that genetic polymorphism is likely to identify relevant genes, that any cytokine gene picked up by a virus must matter and that natural immunosuppressive activity at mucosal surfaces should be worth exploiting, we identify as particularly worthy of attention : ( i ) IL-10, ( ii ) inhibitors of IL-12 production, ( iii ) inhibitors of CD40 ligand expression and ( iv ) oral and nasal tolerance
Yoshimoto et al., Biochem Biophys Res Commun 1998 (Burkitt Lymphoma...) : These results suggest that transformation of primary B cells with EBV induces IL-12 expression potentially through LMP1 expression
Revy et al., Eur J Immunol 1998 (Immunologic Deficiency Syndromes) : We show that the CD40 induced production of IL-6, IL-8 and TNF-alpha by monocytes, and IL-12 by dendritic cells, and expression of the activation markers CD83, the costimulatory molecules CD86 and CD80, and HLA-DR antigens were all similar in patient and control cells
Ouyang et al., Immunity 1998 : Here, we show that loss of GATA-3 expression by developing Th1 cells requires IL-12 signaling through Stat4 and does not simply result from an absence of IL-4
Snijders et al., Int Immunol 1998 : IL-12 production by antigen presenting cells (APC) can be induced by the interaction between CD40 on the APC and CD40 ligand (CD40L) expressed on T cells after activation
Jander et al., J Neuroimmunol 1998 (Encephalomyelitis, Autoimmune, Experimental) : Interleukin (IL)-12 in its heterodimeric p35/p40 isoform and the recently described cytokine IL-18 potently induce T cell production of IFN-gamma
Suzuki et al., J Virol 1999 : Among them, interleukin-12 (IL-12) and IL-4 induce naive CD4 ( + ) T cells to become T-helper 1 ( Th1 ) or Th2 cells, respectively
Pai et al., Oncol Res 1998 : We investigated the regulatory effect of interleukin (IL) 10 on IL-12-inducible killer activity of blood mononuclear cells ( MNC ) of healthy subjects
Hayakawa et al., Biol Pharm Bull 1998 (Liver Neoplasms, Experimental) : Experiments using macrophage- or NK cell-deficient mice revealed that CAE induced IL-12 in serum was not mediated by macrophages and that IFN-gamma production was mainly dependent on natural killer ( NK ) cells
Noguchi et al., Eur J Immunol 1998 : CD4+ and CD8+ T cells from LPS injected GM-CSF-/- mice showed a deficiency in IFN-gamma production and proliferative activity in response to IL-2 and IL-12 , whereas IFN-gamma production by NK cells was not compromised
Ding et al., J Immunol 1998 : Enhancement of costimulation by engagement of CD28 only resulted in augmentation of the capacity of the weak agonist APL to induce proliferation and IL-2/IL-3 production, but not CD40L or IL-12Rbeta2 chain expression on T cells, CD80/CD86 expression on APC, IL-12 secretion, or IFN-gamma production ... Exogenous IL-12 promoted IFN-gamma production in the presence of the weak agonists ... The provision of excess costimulation can overcome some of the defects in T cell activation by weak agonists, but is insufficient to induce a sufficient level of CD40L expression needed for engagement of CD40 on APC with subsequent IL-12 production and induction of IL-12Rbeta2 chain expression ... The provision of excess costimulation can overcome some of the defects in T cell activation by weak agonists, but is insufficient to induce a sufficient level of CD40L expression needed for engagement of CD40 on APC with subsequent IL-12 production and induction of IL-12Rbeta2 chain expression
Schultze et al., J Exp Med 1999 : Although IL-12 was cloned from human Epstein-Barr virus ( EBV ) -transformed B cell lines, surprisingly, CD40 ligation on murine B cells did not lead to IL-12 production, suggesting that murine B cells do not produce IL-12 ... CD40 ligation and interferon (IFN)-gamma exhibited synergistic effects on IL-12 production, whereas IL-10 abrogated and IL-4 significantly inhibited IL-12 production by these B cells ... CD40 ligation and interferon (IFN)-gamma exhibited synergistic effects on IL-12 production, whereas IL-10 abrogated and IL-4 significantly inhibited IL-12 production by these B cells ... CD40 ligation and interferon (IFN)-gamma exhibited synergistic effects on IL-12 production, whereas IL-10 abrogated and IL-4 significantly inhibited IL-12 production by these B cells ... CD40 ligation and interferon (IFN)-gamma exhibited synergistic effects on IL-12 production, whereas IL-10 abrogated and IL-4 significantly inhibited IL-12 production by these B cells
Sasama et al., Int Arch Allergy Immunol 1998 : IL-12 and IFN-gamma had no effect on the amount of IL-4 secreted by PMA and ionomycin stimulated CD45RO CD4 T cells, but culture with IL-4 enhanced IL-4 production 7-fold
Séguin et al., J Infect Dis 1999 : During Toxoplasma gondii infection, host immunity is mediated by interferon-gamma , which is induced by interleukin-12 (IL-12) ... Ligation of CD40 : CD154 was partially essential for IL-12 secretion
Marinaro et al., J Immunol 1999 : CT inhibited IL-12 induced IFN-gamma secretion both in vivo and in vitro ... Mucosal secretory IgA responses to oral or nasal vaccines were not affected by intranasal IL-12
Boyaka et al., J Immunol 1999 (Tetanus) : In summary, both intranasal IL-6 and IL-12 induced serum Abs that protected mice from systemic challenge with TT, whereas only IL-12 induced mucosal S-IgA Ab responses
Wang et al., J Immunol 1999 : This activation of STAT4 in response to IL-2 is not due to the autocrine production of IL-12 or IFN-alpha
Filion et al., Br J Cancer 1999 (Urinary Bladder Neoplasms) : We have found that, although MCC is a potent inducer of IL-12 and IL-6 synthesis in monocytes and macrophages either in vitro or in vivo, it is unable to induce the synthesis of either IL-12, IL-6 or granulocyte-macrophage colony stimulating factor ( GM-CSF ) by the human transitional bladder cancer cell lines HT-1197 and HT-1376
Becher et al., J Immunol 1999 : Based on previous work that suggests that the production of IL-12 by activated human central nervous system derived microglia is regulated by autocrine TNF-alpha , we wanted to determine whether inhibition of TNF could induce a reduction of Th1 responses by its impact on systemic APCs
Wu et al., J Immunol 1999 : One transcriptional factor of the IFN regulatory factor family found to be critical in regulating IL-12 dependent IFN-gamma production in vivo following infectious challenge has been designated IFN consensus sequence binding protein ( ICSBP )
Isler et al., Am J Respir Cell Mol Biol 1999 : The autocrine regulation of the heterodimeric, biologically active form of IL-12 ( IL-12 p70 ) by IL-10 was studied, as well as the expression of its subunits of 35 kD ( p35 ) and 40 kD ( p40 ) ... However, IL-12 p70 was released when the autocrine production of IL-10 was neutralized by IL-10 blocking antibody, and IL-12 p40 production increased
Ulchaker et al., J Immunother 1999 (Carcinoma, Renal Cell...) : The induction of interferon (IFN)-gamma by IL-12 in tumor bearing mice plays an important role in its antitumor activity ... We therefore examined the effects of IL-12 on IFN-gamma production by T cells derived from patients with renal cell carcinoma (RCC), including freshly isolated tumor infiltrating lymphocytes ( T-TIL ), matched peripheral blood T cells ( T-PBL ), and RCC-specific TIL lines ... IL-12 alone induced IFN-gamma secretion by T cells from normal individuals and appeared to act synergistically with either IL-2 or anti-CD3 antibody ... A selective loss of IL-12 dependent production of IFN-gamma was also consistently observed in two of three established RCC-specific TIL lines ... The observed decreases in IFN-gamma production in response to IL-12 may have a negative effect on the development of T-cell immunity
Hirohata et al., Clin Exp Immunol 1999 : The IL-12 induced enhancement of CD40L expression on anti-CD3 activated CD4+ T cells was not influenced in the presence of a metalloproteinase inhibitor KB8301, which up-regulated CD40L expression by preventing the processing of membrane bound CD40L, or B cells, which down-regulated CD40L expression by receptor mediated endocytosis ... These results indicate that IL-12 enhances the CD40L expression of activated CD4+ T cells independently of the IFN-gamma production
Aloisi et al., J Immunol 1999 : CD40-CD154 interaction and IFN-gamma are required for IL-12 but not prostaglandin E2 secretion by microglia during antigen presentation to Th1 cells ... CD40-CD154 interaction and IFN-gamma are required for IL-12 but not prostaglandin E2 secretion by microglia during antigen presentation to Th1 cells ... CD40-CD154 interaction and IFN-gamma are required for IL-12 but not prostaglandin E2 secretion by microglia during antigen presentation to Th1 cells
Retini et al., J Immunol 1999 : The data of this study showed that : 1 ) appreciable levels of IL-12 were observed when freshly isolated monocytes were exposed to acapsular C. neoformans or Candida albicans and secretion occurred within 24-48 h of incubation ; 2 ) monocytes alone were poor producers of IL-12 when stimulated with encapsulated C. neoformans ; 3 ) the presence of specific anti-glucuronoxylomannan mAb favored IL-12 secretion and Fc cross linking could play a role ; 4 ) monocytes were able to secrete consistent levels of IL-12 when cultured with activated T cells responding to C. neoformans ; 5 ) the maximum secretion of IL-12 was observed at 5-7 days of culture and was strongly regulated by the presence of endogenous IFN-gamma ; and 6 ) the interaction between CD40 on monocytes and CD40 ligand on activated T lymphocytes responding to C. neoformans played a critical role in IL-12 secretion ... The data of this study showed that : 1 ) appreciable levels of IL-12 were observed when freshly isolated monocytes were exposed to acapsular C. neoformans or Candida albicans and secretion occurred within 24-48 h of incubation ; 2 ) monocytes alone were poor producers of IL-12 when stimulated with encapsulated C. neoformans ; 3 ) the presence of specific anti-glucuronoxylomannan mAb favored IL-12 secretion and Fc cross linking could play a role ; 4 ) monocytes were able to secrete consistent levels of IL-12 when cultured with activated T cells responding to C. neoformans ; 5 ) the maximum secretion of IL-12 was observed at 5-7 days of culture and was strongly regulated by the presence of endogenous IFN-gamma ; and 6 ) the interaction between CD40 on monocytes and CD40 ligand on activated T lymphocytes responding to C. neoformans played a critical role in IL-12 secretion
Hyodo et al., J Immunol 1999 : These data suggested that NK cells develop and express IL-12R and IL-18R in the absence of IL-12 or IL-18, and that both IL-18 and IL-12 directly and independently augment perforin mediated cytotoxic activity of NK cells ... These data suggested that NK cells develop and express IL-12R and IL-18R in the absence of IL-12 or IL-18, and that both IL-18 and IL-12 directly and independently augment perforin mediated cytotoxic activity of NK cells
Yssel et al., Clin Exp Allergy 1998 (Asthma...) : Both IL-4 and IL-13 are inducing IgE synthesis whereas IFN-gamma and IL-12 are blocking IgE synthesis ... Both IL-4 and IL-13 are inducing IgE synthesis whereas IFN-gamma and IL-12 are blocking IgE synthesis