Gene interactions and pathways from curated databases and text-mining
J Biol Chem 1999, PMID: 9890938

Identification of a STAT4 binding site in the interleukin-12 receptor required for signaling.

Naeger, L K; McKinney, J; Salvekar, A; Hoey, T

The specificity of the various STAT SH2 domains for different tyrosine-containing peptides enables cytokines to activate different signaling pathways and to induce distinct patterns of gene expression. We show that STAT4 has a unique peptide specificity and binds to the peptide sequence pYLPSNID (where pY represents phosphotyrosine). This motif is found at tyrosine residue 800 in the beta2 subunit of the interleukin-12 receptor and is required for DNA binding and transcriptional activity of STAT4. Our data demonstrate that transfection of interleukin-12 receptor beta1 and beta2 subunits is sufficient for STAT4 activation but not for STAT1 or STAT3 activation.

Document information provided by NCBI PubMed

Text Mining Data

Dashed line = No text mining data

Manually curated Databases

  • IRef Intact Interaction: IL12RB2 — STAT4 (association, fluorescence polarization spectroscopy)
  • NCI Pathway Database IL12-mediated signaling events: STAT4 (STAT4) → STAT4 (dimer) complex (STAT4) (modification, collaborate)
    Evidence: mutant phenotype, assay, other species
  • NCI Pathway Database IL12-mediated signaling events: STAT4 (STAT4) → IL12/IL12R/TYK2/JAK2 complex (IL12A-IL12B-IL12RB1-IL12RB2-TYK2-JAK2) (modification, collaborate)
    Evidence: mutant phenotype, assay, other species
  • NCI Pathway Database IL12-mediated signaling events: STAT4 (STAT4) → RIP2 (RIPK2) (modification, collaborate)
    Evidence: mutant phenotype, assay, other species
  • NCI Pathway Database IL12-mediated signaling events: IL12/IL12R/TYK2/JAK2 complex (IL12A-IL12B-IL12RB1-IL12RB2-TYK2-JAK2) → RIP2 (RIPK2) (modification, activates)
    Evidence: mutant phenotype, assay, other species
In total, 29 gene pairs are associated to this article in curated databases