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EGF — RAC1
Pathways - manually collected, often from reviews:
Text-mined interactions from Literome
Gay et al., J Biol Chem 1999
:
In the current study, we establish that these inhibitors inhibit hepatocyte growth factor/scatter factor induced A431 and Madin-Darby canine kidney cell motility and various cell motility related events, including
epidermal growth factor induced ruffling of A431 cells and epidermal growth factor induced translocation of the small GTPase
Rac in these cells
Liu et al., Mol Cell Biol 2000
:
Expression of a carboxy-terminal fragment of Vav2 decreased the elevation of
Rac1 activity
induced by
epidermal growth factor , consistent with Vav2 mediating activation of Rac1 downstream from growth factor receptors
Marignani et al., J Cell Biol 2001
:
Studies using this mutant indicate that Vav2 is not necessary for platelet derived growth factor- or
epidermal growth factor dependent
activation of
Rac
Gildea et al., Cancer Res 2002
(Neoplasm Metastasis...) :
Here we show that
EGF stimulates motility in human tumor cell lines, which harbor activating Ha-RasV12 via a novel signal transduction pathway mediated by the small GTP binding proteins RalA and RhoA but
independent of
Rac1 and Cdc42
Masuda-Robens et al., Mol Cell Biol 2003
:
Epidermal growth factor (EGF) induced relocalization of TRE17 to the plasma membrane in a
Cdc42-/Rac1 dependent manner
Menna et al., Cancer Res 2003
(Mammary Neoplasms, Experimental...) :
Reduced
Rac-GTP levels were observed upon
stimulation with
epidermal growth factor in the beta-GAP clones compared with control cells
Maddala et al., Molecular vision 2003
:
Rac GTPase activation, in contrast, was significantly enhanced in
response to only
EGF or b-FGF
Marcoux et al., Oncogene 2003
:
EGF receptor
mediates adhesion dependent activation of the
Rac GTPase : a role for phosphatidylinositol 3-kinase and Vav2
Zahir et al., J Cell Biol 2003
(Breast Neoplasms...) :
Instead, the cytoplasmic tail of beta4 integrin is necessary for basal and
epidermal growth factor induced
RAC activity, and RAC mediates tumor survival
Huang et al., Cell Mol Life Sci 2005
:
In addition, emodin markedly suppressed
EGF induced activation of Cdc42 and
Rac1 and the corresponding cytoskeleton changes
Zuo et al., Arterioscler Thromb Vasc Biol 2005
(Hypertrophy) :
Knockdown of Cav1 protein by small interfering RNA ( siRNA ) inhibits Ang II-stimulated
Rac1 activation and membrane translocation, H2O2 production, ROS dependent
epidermal growth factor receptor (EGF-R) transactivation , and subsequent phosphorylation of Akt without affecting ROS independent extracellular signal regulated kinase 1/2 phosphorylation
Jenei et al., Exp Cell Res 2005
:
In the present study, we found that overexpression of e3B1 in NIH3T3/EGFR cells sensitized
EGF induced
activation of
Rac1 , whereas it had no impact on EGF induced activation of p21Ras
Murai et al., Biochem J 2006
:
These results indicate that
EGF induces ADAM10 mediated CD44 cleavage through
Rac1 and mitogen activated protein kinase activation, and thereby promotes tumour cell migration and invasion
Ray et al., Cell Motil Cytoskeleton 2007
:
In the present study, we demonstrate that MEK activation by
EGF increased
Rac1 activation, dissociation of intercellular contacts, and migration in both control and polyamine depleted cells, while U0126, a specific inhibitor of MEK1, prevented disruption of junctions as well as EGF induced Rac1 activation
Beier et al., Atherosclerosis 2008
:
However, the
effect of
EGF on
Rac/Cdc42 activation is unknown for VSMC ... In the present report, we evaluated
stimulation of
Rac/Cdc42 by
EGF in VSMC performing PAK-PBD binding assay ...
EGF treatment of VSMC
induced time and concentration dependent binding of GTP bound
Rac1 to PAK-PBD peaking at 1 min and showing sustained activation up to 15 min ... In VSMC, EGF sequentially stimulated PAK, peaking at 5 min, and JNK, peaking at 15 min. Pretreatment of VSMC by EGF receptor specific tyrosine kinase inhibitor AG1478 and non-specific tyrosine kinase inhibitor genistein inhibited
EGF induced activation of
Rac1 , PAK and JNK, whereas tyrosine kinase inhibitors specific for Src ( PP1 ) and specific for platelet derived growth factor ( AG1296 ) had no effect ... Our data provide evidence for
EGF induced
Rac1 activation and implicate PAK and JNK as downstream targets of Rac1 in EGF signal transduction in VSMC
Wu et al., Mol Med 2007
(Neoplasm Recurrence, Local...) :
Up-regulation of
Rac1 by
epidermal growth factor mediates COX-2 expression in recurrent respiratory papillomas
Dise et al., Am J Physiol Gastrointest Liver Physiol 2008
:
Whereas EGF failed to activate Rac in colon cells from EGF receptor (EGFR) knockout mice, stable expression of wild-type EGFR restored
EGF stimulated
Rac activation and migration ... Pharmacological inhibition of either phosphatidylinositol 3-kinase (PI3K) or Src family kinases reduced
EGF stimulated
Rac activation ... Cotreatment of cells with both inhibitors completely blocked
EGF stimulated
Rac activation and localization to the leading edge of cells and lamellipodial extension
Itoh et al., J Cell Sci 2008
:
Because knockdown of these GEFs suppressed
EGF induced
Rac1 activation only partially, we looked for another Rac1 GEF downstream of the EGF receptor and found that Asef, a Rac1-Cdc42 GEF bound to the tumor suppressor APC, also contributed to EGF induced Rac1 activation ... The suppression of
EGF induced
Rac1 activation in siRNA treated cells was restored by wild-type Asef, but not by the Tyr94Phe mutant of Asef
Li et al., Mol Endocrinol 2009
:
Here we showed that PLC-gamma1 forms a complex with
Rac1 in
response to
EGF ... This interaction is critical for
EGF induced
Rac1 activation in vivo, and PLC-gamma1 SH3 domain is actually a potent and specific Rac1 guanine nucleotide exchange factor in vitro
Everett et al., J Biol Chem 2009
:
Analysis of PLC gamma 1 and PLC gamma 2 with point mutations of this residue showed that removal of the negative charge enhanced PLC activity in response to
EGF stimulation or
activation by
Rac
Wang et al., Nan Fang Yi Ke Da Xue Xue Bao 2010
:
The packaged lentiviral titer was 1x10 ( 6 ) TU/ml. Analysis of Rac1 biological activity showed that Rac1N17 lentivirus particles infection significantly inhibited
epidermal growth factor stimulated
Rac1 activity in the PFCs, while Rac1L61 lentivirus particles enhanced the Rac1 activity
Kobayashi et al., J Biol Chem 2011
:
Here, we report that
EGF induces biphasic
Rac1 activation in the process of cell migration, and UTKO1, a cell migration inhibitor, inhibits the second EGF induced wave of Rac1 activation but not the first wave
Caino et al., PloS one 2012
(Carcinoma...) :
Here we show that RNAi mediated knockdown of PKCe in H358, H1299, H322, and A549 NSCLC impairs activation of the small GTPase
Rac1 in
response to phorbol 12-myristate 13-acetate ( PMA ), serum, or
epidermal growth factor (EGF)
Hu et al., PloS one 2012
(Carcinoma, Hepatocellular...) :
Ectopic expression GEP100 siRNA, GEP100-?PH, or Arf6-T27N suppressed
EGF induced ERK and
Rac1 activity
Xu et al., PloS one 2012
:
Thrombin or
EGF resulted in activation of
Rac1 in CHRF-288-11 or HeLa cells respectively and W7 inhibited this activation
Schwarz et al., PloS one 2012
:
We previously reported about
EGF induced Ser-71 phosphorylation of
Rac1/Cdc42
Kyriakakis et al., Cell Signal 2013
:
Rac1 activation by
EGF was inhibited by gefitinib, lapatinib or SB203580 but amplified by PD98059
Long et al., Arthritis Rheum 2013
(Osteoarthritis) :
EGF and TGFa, but not FN fragments,
increased Rac1 activity and promoted the increase in MMP-13 above that achieved by stimulation with FN fragments alone
Yang et al., Journal of biomedical research 2011
:
Interestingly,
EGF could
induce a significant production of ROS, and N-acetyl-L-cysteine, a scavenger of ROS which abolished the EGF induced ROS generation, cell migration, as well as activation of PI3K/Akt and PAK, but not
Rac1
Hess et al., J Biol Chem 1997
:
In summary, these data indicate a major
role for
Rac1 in the activation of PLD by
EGF , but not PDGF or protein kinase C
Kim et al., FEBS Lett 1997
:
By reporter gene analysis following transient or stable transfections with pEXV-RacN17 encoding a dominant negative mutant of Rac, EGF induced activation of c-fos SRE-luciferase gene was shown to be selectively inhibited, suggesting that
Rac activity is
necessary for the full activation of SRE by
EGF
Malliri et al., J Cell Biol 1998
:
However,
EGF induced translocation of
Rac to the cell membrane, which is associated with its activation, is defective in TA cells