Mol Endocrinol 2009,
PMID: 19264842
Li, Siwei; Wang, Qian; Wang, Yi; Chen, Xinmei; Wang, Zhixiang
It is well established that epidermal growth factor (EGF) induces the cytoskeleton reorganization and cell migration through two major signaling cascades: phospholipase C-gamma1 (PLC-gamma1) and Rho GTPases. However, little is known about the cross talk between PLC-gamma1 and Rho GTPases. Here we showed that PLC-gamma1 forms a complex with Rac1 in response to EGF. This interaction is direct and mediated by PLC-gamma1 Src homology 3 (SH3) domain and Rac1 (106)PNTP(109) motif. This interaction is critical for EGF-induced Rac1 activation in vivo, and PLC-gamma1 SH3 domain is actually a potent and specific Rac1 guanine nucleotide exchange factor in vitro. We have also demonstrated that the interaction between PLC-gamma1 SH3 domain and Rac1 play a significant role in EGF-induced F-actin formation and cell migration. We conclude that PLC-gamma1 and Rac1 coregulate EGF-induced cell cytoskeleton remodeling and cell migration by a direct functional interaction.
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Text Mining Data
PLC-gamma1 → EGF: "
Here we showed that
PLC-gamma1 forms a complex with Rac1 in
response to
EGF
"
Rac1 → EGF: "
Here we showed that PLC-gamma1 forms a complex with Rac1 in response to EGF
"
Rac1 → EGF: "
This interaction is critical for EGF induced Rac1 activation in vivo, and PLC-gamma1 SH3 domain is actually a potent and specific Rac1 guanine nucleotide exchange factor in vitro
"
Manually curated Databases
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IRef Intact Interaction:
RAC1
—
PAK2
(physical association, pull down)
-
IRef Intact Interaction:
RAC1
—
PLCG1
(physical association, anti bait coimmunoprecipitation)
-
IRef Intact Interaction:
RAC1
—
PLCG1
(physical association, anti tag coimmunoprecipitation)
-
IRef Intact Interaction:
RAC1
—
PLCG1
(physical association, pull down)
-
IRef Intact Interaction:
RAC1
—
PLCG1
(direct interaction, pull down)
In total, 2 gene pairs are associated to this article in curated databases