Denisova cave entrance in the Altai Mountains
of Siberia, Russia where the bones were found from which
DNA was sequenced
(Copyright (C) 2010, Johannes Krause)
The Denisova Sequence track shows
high-coverage sequence reads from an archaic Denisovan individual
mapped to the human genome reference assembly.
The Denisova DNA was extracted from a phalanx bone excavated from
Denisova Cave in the Altai Mountains in southern Siberia.
A novel single-stranded DNA library preparation method
supplementary online materials of Meyer, 2012)
was applied to DNA previously extracted from 40mg of bone (Reich, 2010).
Using single-stranded DNA greatly increased the genomic coverage to
30X compared to an earlier 1.9X sequence (Reich, 2010).
Sequence reads were aligned to human sequence Feb. 2009 (GRCh37/hg19)
(downloaded from the
1000 Genomes Project) using the
Thanks to the
Max Planck Institute for Evolutionary Anthropology
for providing the
BAM files used for this track.
Meyer M, Kircher M, Gansauge MT, Li H, Racimo F, Mallick S, Schraiber JG, Jay F, Prüfer K, de
Filippo C et al.
A high-coverage genome sequence from an archaic Denisovan individual.
Science. 2012 Oct 12;338(6104):222-6.
PMID: 22936568; PMC: PMC3617501;
supplementary online materials, Note 2
Reich D, Green RE, Kircher M, Krause J, Patterson N, Durand EY, Viola B, Briggs AW, Stenzel U,
Johnson PL et al.
Genetic history of an archaic hominin group from Denisova Cave in Siberia.
Nature. 2010 Dec 23;468(7327):1053-60.