Swift Primers Track Settings
 
Swift BioSciences sequencing primers   (All Mapping and Sequencing tracks)

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Data last updated at UCSC: 2021-03-11 00:17:31

Description

This track shows the primers for the Swift Amplicon ® SARS-CoV-2 Panel single-tube NGS assay:

This kit leverages patented multiplex PCR technology, enabling library construction from 1st-strand or 2nd-strand cDNA using tiled primer pairs to target the entire 29.9 kb viral genome with a single pool of multiplexed primer pairs. Primers were designed against the NCBI Reference Sequence NC_045512.2 (Severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1, complete genome). In silico analysis predicted zero off-target products from human host genome sequences.

Display Conventions and Configuration

Genomic locations of primers are highlighted. A click on them shows the primer sequence. This is one of the few tracks that may be best displayed in "full" mode.

Methods

Primer sequences, names and genomic locations were downloaded from Swift and converted to bigBed. More details are available from Swift Biosciences.

Data Access

The raw data can be explored interactively with the Table Browser or combined with other datasets in the Data Integrator tool. For automated analysis, the genome annotation is stored in a bigBed file that can be downloaded from the download server.

Annotations can be converted from binary to ASCII text by our command-line tool bigBedToBed. Instructions for downloading this command can be found on our utilities page. The tool can also be used to obtain features within a given range without downloading the file, for example:

bigBedToBed http://hgdownload.soe.ucsc.edu/gbdb/wuhCor1/bbi/swift.bb -chrom=NC_045512v2 -start=0 -end=29902 stdout

Please refer to our mailing list archives for questions, or our Data Access FAQ for more information.