Lung Travaglini Lung Detail FACS Track Settings
 
Lung cells FACS method binned by detailed cell type from Travaglini et al 2020

Track collection: Lung cells from from Travaglini et al 2020

+  Description
+  All tracks in this collection (19)

Display mode:   

Label: Name or ID of item    Alternative name for item   

Log10(x+1) transform:    View limits maximum: count/cell (range 0-10000)

Categories:  
 Adventitial Fibroblast P1
 Adventitial Fibroblast P2
 Adventitial Fibroblast P3
 Airway Smooth Muscle P1
 Airway Smooth Muscle P2
 Airway Smooth Muscle P3
 Alveolar Epithelial Type 1 P1
 Alveolar Epithelial Type 1 P2
 Alveolar Epithelial Type 1 P3
 Alveolar Epithelial Type 2 P1
 Alveolar Epithelial Type 2 P2
 Alveolar Epithelial Type 2 P3
 Alveolar Fibroblast P1
 Alveolar Fibroblast P2
 Alveolar Fibroblast P3
 Artery P1
 Artery P2
 Artery P3
 B P1
 B P2
 B P3
 Basal P1
 Basal P2
 Basal P3
 Basophil/Mast 1 P1
 Basophil/Mast 1 P2
 Basophil/Mast 1 P3
 Bronchial Vessel 1 P1
 CD4+ Memory/Effector T P1
 CD4+ Naive T P1
 CD4+ Naive T P2
 CD8+ Memory/Effector T P1
 CD8+ Naive T P1
 CD8+ Naive T P2
 Capillary Aerocyte P1
 Capillary Aerocyte P2
 Capillary Aerocyte P3
 Capillary Intermediate 1 P2
 Capillary P1
 Capillary P2
 Capillary P3
 Ciliated P1
 Ciliated P2
 Ciliated P3
 Classical Monocyte P1
 Club P1
 Club P2
 Club P3
 Dendritic P1
 Differentiating Basal P3
 Fibromyocyte P3
 Goblet P1
 Goblet P2
 Goblet P3
 IGSF21+ Dendritic P2
 IGSF21+ Dendritic P3
 Intermediate Monocyte P2
 Intermediate Monocyte P3
 Ionocyte P3
 Lipofibroblast P1
 Lymphatic P1
 Lymphatic P2
 Lymphatic P3
 Macrophage P2
 Macrophage P3
 Myeloid Dendritic Type 2 P3
 Myofibroblast P2
 Myofibroblast P3
 Natural Killer T P2
 Natural Killer T P3
 Natural Killer P1
 Natural Killer P2
 Natural Killer P3
 Neuroendocrine P1
 Neuroendocrine P3
 Neutrophil P1
 Neutrophil P2
 Neutrophil P3
 Nonclassical Monocyte P1
 Nonclassical Monocyte P2
 Pericyte P1
 Pericyte P2
 Pericyte P3
 Plasma P3
 Plasmacytoid Dendritic P1
 Plasmacytoid Dendritic P2
 Plasmacytoid Dendritic P3
 Proliferating NK/T P2
 Proliferating NK/T P3
 Signaling Alveolar Epithelial Type 2 P1
 Signaling Alveolar Epithelial Type 2 P3
 Vascular Smooth Muscle P1
 Vascular Smooth Muscle P2
 Vascular Smooth Muscle P3
 Vein P2
View table schema
Data last updated at UCSC: 2022-05-12 17:26:50

Description

This track displays data from A molecular cell atlas of the human lung from single-cell RNA sequencing. Using droplet-based and plate-based single-cell RNA sequencing (scRNA-seq), 58 lung cell type populations were identified: 15 epithelial, 9 endothelial, 9 stromal, and 25 immune. This dataset covers ~75,000 human cells across all lung tissue compartments and circulating blood.

This track collection contains 19 bar chart tracks of RNA expression in the human lung where cells are grouped such as by cell type (Lung Cells, Lung Cells FACS), tissue compartments (Lung Compart, Lung Compart FACS), detailed cell type (Lung Detail, Lung Detail FACS), organ donor (Lung Donor, Lung Donor FACS), halfway detailed cell type (Lung Half Det, Lung Half Det FACS), sample location (Lung Locat, Lung Locat FACS), or organ (Lung Organ, Lung Organ FACS). The default track displayed is Lung Cells.

Display Conventions

The cell types are colored by which class they belong to according to the following table.

Color Cell classification
fibroblast
immune
muscle
secretory
ciliated
epithelial
endothelial

Cells that fall into multiple classes will be colored by blending the colors associated with those classes. The colors will be purest in the Lung Cells subtrack, where the bars represent relatively pure cell types. They can give an overview of the cell composition within other categories in other subtracks as well.

Data Access

The raw bar chart data can be explored interactively with the Table Browser, or the Data Integrator. For automated analysis, the data may be queried from our REST API. Please refer to our mailing list archives for questions, or our Data Access FAQ for more information.

Method

Healthy lung tissue and peripheral blood was surgically removed from 2 male patients (ages 46 and 75) and 1 female patient (age 51) undergoing lobectomy for focal lung tumors. Lung tissue was sampled from the bronchi (proximal), bronchiole (medial), and alveolar (distal) regions. Lung samples were dissociated and enriched with magnetic columns before being sorted into epithelial, endothelial/immune, and stromal cell suspensions. Lung and peripheral blood libraries were prepared using the 10x Genomics 3' v2 kit. In parallel, Smart-Seq2 (SS2) cDNA libraries were prepared using the Nextera XT library kit. Both 10x and SS2 libraries were sequenced on a NovaSeq 6000.

The cell/gene matrix and cell-level metadata was downloaded from the UCSC Cell Browser. The UCSC command line utility matrixClusterColumns, matrixToBarChart, and bedToBigBed were used to transform these into a bar chart format bigBed file that can be visualized. The coloring was done by defining colors for the broad level cell classes and then using another UCSC utility, hcaColorCells, to interpolate the colors across all cell types. The UCSC utilities can be found on our download server.

Data Access

The raw bar chart data can be explored interactively with the Table Browser or the Data Integrator. For automated analysis, the data may be queried from our REST API. Please refer to our mailing list archives for questions, or our Data Access FAQ for more information.

Credit

Thanks to Kyle J. Travaglini, Ahmad N. Nabhan, and to the many authors who worked on producing and publishing this data set. The data were integrated into the UCSC Genome Browser by Jim Kent and Brittney Wick then reviewed by Gerardo Perez. The UCSC work was paid for by the Chan Zuckerberg Initiative.

References

Travaglini KJ, Nabhan AN, Penland L, Sinha R, Gillich A, Sit RV, Chang S, Conley SD, Mori Y, Seita J et al. A molecular cell atlas of the human lung from single-cell RNA sequencing. Nature. 2020 Nov;587(7835):619-625. PMID: 33208946; PMC: PMC7704697