◀ Back to IL3
IL3 — JAK2
Pathways - manually collected, often from reviews:
-
BioCarta il 3 signaling pathway:
IL-3/IL-3R-alpha/IL-3R-beta/JAK2 complex (JAK2-CSF2RB-IL3RA-IL3)
→
STAT5 (STAT5A/STAT5B)
(modification, activates)
-
BioCarta il 3 signaling pathway:
IL-3/IL-3R-alpha/IL-3R-beta/JAK2 complex (JAK2-CSF2RB-IL3RA-IL3)
→
SHC/GRB2/SOS-1 complex (SOS1-GRB2-SHC1)
(modification, activates)
-
BioCarta il 3 signaling pathway:
IL-3/IL-3R-alpha/IL-3R-beta/JAK2 complex (JAK2-CSF2RB-IL3RA-IL3)
→
JAK2
(modification, collaborate)
-
BioCarta il 3 signaling pathway:
IL-3/IL-3R-alpha/IL-3R-beta/JAK2 complex (JAK2-CSF2RB-IL3RA-IL3)
→
IL-3/IL-3R-alpha/IL-3R-beta complex (IL3RA-IL3-CSF2RB)
(modification, collaborate)
-
BioCarta il 3 signaling pathway:
JAK2
→
IL-3/IL-3R-alpha/IL-3R-beta complex (IL3RA-IL3-CSF2RB)
(modification, collaborate)
-
NCI Pathway Database IL3-mediated signaling events:
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2/SHC complex (CSF2RB-JAK2-SHC1-IL3-IL3RA)
→
SHIP (INPP5D)
(modification, collaborate)
Bone et al., Cell Signal 2000, Damen et al., Proc Natl Acad Sci U S A 1996, Lioubin et al., Genes Dev 1996
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database IL3-mediated signaling events:
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2/SHC complex (CSF2RB-JAK2-SHC1-IL3-IL3RA)
→
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2/SHC/SHIP complex (CSF2RB-JAK2-SHC1-INPP5D-IL3-IL3RA)
(modification, collaborate)
Bone et al., Cell Signal 2000, Damen et al., Proc Natl Acad Sci U S A 1996, Lioubin et al., Genes Dev 1996
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database IL3-mediated signaling events:
SHIP (INPP5D)
→
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2/SHC/SHIP complex (CSF2RB-JAK2-SHC1-INPP5D-IL3-IL3RA)
(modification, collaborate)
Bone et al., Cell Signal 2000, Damen et al., Proc Natl Acad Sci U S A 1996, Lioubin et al., Genes Dev 1996
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database IL3-mediated signaling events:
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2/SHP2 complex (CSF2RB-JAK2-PTPN11-IL3-IL3RA)
→
GRB2/GAB2 complex (GRB2-GAB2)
(modification, collaborate)
Edmead et al., Cell Signal 2006, Welham et al., J Biol Chem 1994, Gu et al., J Biol Chem 1997, Craddock et al., J Biol Chem 1997, Gu et al., Mol Cell 1998
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database IL3-mediated signaling events:
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2/SHP2 complex (CSF2RB-JAK2-PTPN11-IL3-IL3RA)
→
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2/SHP2/GRB2/GAB2 complex (CSF2RB-JAK2-PTPN11-IL3-IL3RA-GRB2-GAB2)
(modification, collaborate)
Edmead et al., Cell Signal 2006, Welham et al., J Biol Chem 1994, Gu et al., J Biol Chem 1997, Craddock et al., J Biol Chem 1997, Gu et al., Mol Cell 1998
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database IL3-mediated signaling events:
GRB2/GAB2 complex (GRB2-GAB2)
→
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2/SHP2/GRB2/GAB2 complex (CSF2RB-JAK2-PTPN11-IL3-IL3RA-GRB2-GAB2)
(modification, collaborate)
Edmead et al., Cell Signal 2006, Welham et al., J Biol Chem 1994, Gu et al., J Biol Chem 1997, Craddock et al., J Biol Chem 1997, Gu et al., Mol Cell 1998
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database IL3-mediated signaling events:
JAK2 (JAK2)
→
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2 complex (CSF2RB-JAK2-IL3-IL3RA)
(modification, collaborate)
Quelle et al., Mol Cell Biol 1994
Evidence: assay
-
NCI Pathway Database IL3-mediated signaling events:
JAK2 (JAK2)
→
IL3/IL3RA/CSF2RB (dodecamer) complex (CSF2RB-IL3-IL3RA)
(modification, collaborate)
Quelle et al., Mol Cell Biol 1994
Evidence: assay
-
NCI Pathway Database IL3-mediated signaling events:
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2 complex (CSF2RB-JAK2-IL3-IL3RA)
→
IL3/IL3RA/CSF2RB (dodecamer) complex (CSF2RB-IL3-IL3RA)
(modification, collaborate)
Quelle et al., Mol Cell Biol 1994
Evidence: assay
-
NCI Pathway Database IL3-mediated signaling events:
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2/SHC complex (CSF2RB-JAK2-SHC1-IL3-IL3RA)
→
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2/SHC/GRB2/GAB2 complex (CSF2RB-JAK2-SHC1-GRB2-GAB2-IL3-IL3RA)
(modification, collaborate)
Bone et al., Cell Signal 2000, Gu et al., Mol Cell Biol 2000, Edmead et al., Cell Signal 2006, Gu et al., Mol Cell 1998
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database IL3-mediated signaling events:
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2/SHC complex (CSF2RB-JAK2-SHC1-IL3-IL3RA)
→
GRB2/GAB2 complex (GRB2-GAB2)
(modification, collaborate)
Bone et al., Cell Signal 2000, Gu et al., Mol Cell Biol 2000, Edmead et al., Cell Signal 2006, Gu et al., Mol Cell 1998
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database IL3-mediated signaling events:
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2/SHC/GRB2/GAB2 complex (CSF2RB-JAK2-SHC1-GRB2-GAB2-IL3-IL3RA)
→
GRB2/GAB2 complex (GRB2-GAB2)
(modification, collaborate)
Bone et al., Cell Signal 2000, Gu et al., Mol Cell Biol 2000, Edmead et al., Cell Signal 2006, Gu et al., Mol Cell 1998
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database IL3-mediated signaling events:
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2 complex (CSF2RB-JAK2-IL3-IL3RA)
→
STAT5 (STAT5A/STAT5B)
(modification, activates)
Azam et al., EMBO J 1995, Chin et al., Blood 1996
Evidence: assay, physical interaction
-
NCI Pathway Database IL3-mediated signaling events:
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2 complex (CSF2RB-JAK2-IL3-IL3RA)
→
STAT5-active (STAT5A/STAT5B)
(modification, activates)
Azam et al., EMBO J 1995, Chin et al., Blood 1996
Evidence: assay, physical interaction
-
NCI Pathway Database IL3-mediated signaling events:
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2/SHP2 complex (CSF2RB-JAK2-PTPN11-IL3-IL3RA)
→
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2 complex (CSF2RB-JAK2-IL3-IL3RA)
(modification, collaborate)
Welham et al., J Biol Chem 1994, Bone et al., J Biol Chem 1997
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database IL3-mediated signaling events:
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2/SHP2 complex (CSF2RB-JAK2-PTPN11-IL3-IL3RA)
→
SHP2 (PTPN11)
(modification, collaborate)
Welham et al., J Biol Chem 1994, Bone et al., J Biol Chem 1997
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database IL3-mediated signaling events:
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2 complex (CSF2RB-JAK2-IL3-IL3RA)
→
SHP2 (PTPN11)
(modification, collaborate)
Welham et al., J Biol Chem 1994, Bone et al., J Biol Chem 1997
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database IL3-mediated signaling events:
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2/SHC/GRB2/GAB2 complex (CSF2RB-JAK2-SHC1-GRB2-GAB2-IL3-IL3RA)
→
PI3K complex (PIK3CA-PIK3R1)
(modification, collaborate)
Gu et al., Mol Cell Biol 2000, Gu et al., Mol Cell 1998
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database IL3-mediated signaling events:
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2/SHC/GRB2/GAB2 complex (CSF2RB-JAK2-SHC1-GRB2-GAB2-IL3-IL3RA)
→
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2/SHC/GRB2/GAB2/PI3K complex (CSF2RB-JAK2-SHC1-GRB2-GAB2-IL3-IL3RA-PIK3CA-PIK3R1)
(modification, collaborate)
Gu et al., Mol Cell Biol 2000, Gu et al., Mol Cell 1998
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database IL3-mediated signaling events:
PI3K complex (PIK3CA-PIK3R1)
→
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2/SHC/GRB2/GAB2/PI3K complex (CSF2RB-JAK2-SHC1-GRB2-GAB2-IL3-IL3RA-PIK3CA-PIK3R1)
(modification, collaborate)
Gu et al., Mol Cell Biol 2000, Gu et al., Mol Cell 1998
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database IL3-mediated signaling events:
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2/SHC complex (CSF2RB-JAK2-SHC1-IL3-IL3RA)
→
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2 complex (CSF2RB-JAK2-IL3-IL3RA)
(modification, collaborate)
Bone et al., Cell Signal 2000
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database IL3-mediated signaling events:
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2/SHC complex (CSF2RB-JAK2-SHC1-IL3-IL3RA)
→
SHC (SHC1)
(modification, collaborate)
Bone et al., Cell Signal 2000
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database IL3-mediated signaling events:
IL3/IL3RA/CSF2RB (dodecamer)/JAK2/JAK2 complex (CSF2RB-JAK2-IL3-IL3RA)
→
SHC (SHC1)
(modification, collaborate)
Bone et al., Cell Signal 2000
Evidence: mutant phenotype, physical interaction
-
Reactome Reaction:
IL3
→
JAK2
(indirect_complex)
Orban et al., Blood 1999*, Kitamura et al., Cell 1991
-
Reactome Reaction:
IL3
→
JAK2
(reaction)
Orban et al., Blood 1999*, Sakamaki et al., EMBO J 1992, Duronio et al., J Biol Chem 1992, Kitamura et al., Cell 1991, Sorensen et al., J Biol Chem 1989, Quelle et al., Mol Cell Biol 1994, Feng et al., Mol Cell Biol 1997, Bone et al., J Biol Chem 1997, Flores-Morales et al., Mol Cell Endocrinol 1998
Text-mined interactions from Literome
Le et al., J Biol Chem 2000
(MAP Kinase Signaling System) :
Moreover, the mutations abolished
IL-3 induced
JAK2 , STAT, and AKT activation in the unselected cells, whereas activation of these molecules in IL-3 selected cells was normal
Yokoyama et al., J Interferon Cytokine Res 2001
:
The association between Jak2 and PP2A transiently increases following
interleukin-3 (IL-3) stimulation and
activation of
Jak2
Miura et al., J Immunol 2001
(MAP Kinase Signaling System) :
However, only
IL-3 and IL-5
induced Janus kinase 2 and STAT5 phosphorylation
Ungureanu et al., Mol Cell Biol 2002
:
In unstimulated cells
Jak2 was monoubiquitinated, and
interleukin-3 or gamma interferon stimulation
induced polyubiquitination of Jak2
Yu et al., Oncogene 2003
:
Further biochemical analyses revealed that
IL-3 induced
Jak/Stat , Erk, and PI3 kinase pathways in SHP-2 ( -/- ) cells were impaired and reintroduction of WT SHP-2 into mutant cells partially restored IL-3 signaling
Natarajan et al., Glia 2004
(Encephalitis...) :
In this study, we found that in vitro treatment of EOC-20 microglial cells with tyrphostin AG490 blocked
IL-3 induced tyrosine phosphorylation of
JAK2 , STAT5A, and STAT5B signaling proteins ... Stable transfection of EOC-20 cells with a dominant negative JAK2 mutant also blocked
IL-3 induced tyrosine phosphorylation of
JAK2 , STAT5A, and STAT5B in microglia
To et al., Br J Cancer 2004
(Cell Transformation, Neoplastic...) :
The
interleukin mediated
Janus kinase ( JAK ) /STAT pathway plays a crucial role in carcinogenesis
Huang et al., J Cell Biochem 2005
:
IL-3 induces tyrosine phosphorylation of both JAK1 and
JAK2
Pecaric-Petkovic et al., Blood 2009
(Hypersensitivity) :
These effects are similar to that of IL-3, but the signaling pathways engaged are distinct because IL-33 strongly activates NF-kappaB and shows a preference for p38 MAP-kinase, while
IL-3 acts through
Jak/Stat and preferentially activates ERK
Kim et al., Molecular cancer 2010
:
In addition, treatment of 32D/IL-2Rbeta cells with the compound led to a block in IL-2 dependent activation of JAK3/STAT5 but not
IL-3 dependent
activation of
JAK2/STAT5
Pradhan et al., J Biol Chem 2010
:
In addition, expression of components of the interleukin-3 receptor,
IL3Ra and the common beta chain,
activated JAK2-V617F as well as STAT5 and ERK
Jatiani et al., Genes & cancer 2010
:
ON044580 inhibits mutant JAK2 kinase and the proliferation of JAK2 ( V617F ) -positive leukemic cells and blocks the
IL-3 mediated phosphorylation of
JAK2 and STAT5
Kandala et al., Drug Discov Ther 2012
(Ovarian Neoplasms) :
IL-3 induced phosphorylation of
JAK2 and STAT3 was significantly blocked by DIM
Tang et al., Mol Cell Biol 1994
:
Conversely, the cytoplasmic kinase
JAK2 is
activated by
IL-3 but not by SCF stimulation
Mui et al., J Leukoc Biol 1995
:
Because
IL-3 activates
JAK2 , we searched for the STAT(s) that might transduce IL-3 signals
Silvennoinen et al., J Immunol 1996
:
Finally, CD38 ligation did not inhibit
IL-3 induced tyrosine phosphorylation of
Jak2
O'Farrell et al., Blood 1996
:
IL-3 , but not SCF,
leads to activation of
Jak 2 and Stat 5 and induces pim-1 expression
Nagata et al., Biochem Biophys Res Commun 1996
:
Interleukin 3 activates not only
JAK2 and STAT5, but also Tyk2, STAT1, and STAT3 ... It has been described that
interleukin 3 (IL3) activates
JAK2 , which in turn stimulates STAT5 activation
Kouro et al., Int Immunol 1996
:
In the studies described here, we demonstrate that IL-5,
IL-3 or GM-CSF stimulation
induces the tyrosine phosphorylation of
JAK2 , and to a lesser extent JAK1, and of STAT5
Watanabe et al., J Allergy Clin Immunol 1996
:
It is well documented that
IL-3 or GM-CSF
activate JAK2 in BA/F3 cells
Chin et al., Blood 1997
:
It was also shown that
IL-3 induces rapid and dose dependent association of
Jak2 with betaIL3