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IL5 — IL5RA

Pathways - manually collected, often from reviews:

• KEGG Cytokine-cytokine receptor interaction: IL5 → Complex of CSF2RB-IL5RA (protein-protein, activation)
• NCI Pathway Database IL5-mediated signaling events: IL5 (IL5) → IL5RA/Syntenin complex (IL5RA-SDCBP) (modification, collaborate)
  Czabotar et al., Cytokine 2000*, Geijsen et al., Science 2001*, Tavernier et al., Proc Natl Acad Sci U S A 1992, Murata et al., J Exp Med 1992*, Tavernier et al., Cell 1991, Beekman et al., Blood 2009*, Devos et al., EMBO J 1991, Johanson et al., J Biol Chem 1995, Milburn et al., Nature 1993*, Takaki et al., J Exp Med 1993*, Li et al., Proc Natl Acad Sci U S A 1997*
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL5-mediated signaling events: IL5 (IL5) → IL5/IL5RA/Syntenin complex (IL5RA-SDCBP-IL5) (modification, collaborate)
  Czabotar et al., Cytokine 2000*, Geijsen et al., Science 2001*, Tavernier et al., Proc Natl Acad Sci U S A 1992, Murata et al., J Exp Med 1992*, Tavernier et al., Cell 1991, Beekman et al., Blood 2009*, Devos et al., EMBO J 1991, Johanson et al., J Biol Chem 1995, Milburn et al., Nature 1993*, Takaki et al., J Exp Med 1993*, Li et al., Proc Natl Acad Sci U S A 1997*
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL5-mediated signaling events: IL5RA/Syntenin complex (IL5RA-SDCBP) → IL5/IL5RA/Syntenin complex (IL5RA-SDCBP-IL5) (modification, collaborate)
  Czabotar et al., Cytokine 2000*, Geijsen et al., Science 2001*, Tavernier et al., Proc Natl Acad Sci U S A 1992, Murata et al., J Exp Med 1992*, Tavernier et al., Cell 1991, Beekman et al., Blood 2009*, Devos et al., EMBO J 1991, Johanson et al., J Biol Chem 1995, Milburn et al., Nature 1993*, Takaki et al., J Exp Med 1993*, Li et al., Proc Natl Acad Sci U S A 1997*
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL5-mediated signaling events: IL5/IL5RA/CSF2RB (hexamer)/JAK2/JAK2 complex (CSF2RB-IL5-IL5RA-JAK2) → STAT5 (STAT5A/STAT5B) (modification, activates) Mui et al., EMBO J 1995, Kouro et al., Int Immunol 1996
  Evidence: assay
• NCI Pathway Database IL5-mediated signaling events: IL5/IL5RA/CSF2RB (hexamer)/JAK2/JAK2 complex (CSF2RB-IL5-IL5RA-JAK2) → STAT5-active (STAT5A/STAT5B) (modification, activates)
  Mui et al., EMBO J 1995, Kouro et al., Int Immunol 1996
  Evidence: assay
• NCI Pathway Database IL5-mediated signaling events: LYN (LYN) → IL5/IL5RA/CSF2RB (hexamer) complex (CSF2RB-IL5-IL5RA) (modification, collaborate)
  Adachi et al., J Immunol 1999, Stafford et al., J Immunol 2002*, Sato et al., J Exp Med 1994, Adachi et al., J Immunol 1999
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database IL5-mediated signaling events: LYN (LYN) → IL5/IL5RA/CSF2RB (hexamer)/LYN complex (CSF2RB-IL5-IL5RA-LYN) (modification, collaborate)
  Adachi et al., J Immunol 1999, Stafford et al., J Immunol 2002*, Sato et al., J Exp Med 1994, Adachi et al., J Immunol 1999
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database IL5-mediated signaling events: IL5/IL5RA/CSF2RB (hexamer) complex (CSF2RB-IL5-IL5RA) → IL5/IL5RA/CSF2RB (hexamer)/LYN complex (CSF2RB-IL5-IL5RA-LYN) (modification, collaborate)
  Adachi et al., J Immunol 1999, Stafford et al., J Immunol 2002*, Sato et al., J Exp Med 1994, Adachi et al., J Immunol 1999
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database IL5-mediated signaling events: GRB2 (GRB2) → IL5/IL5RA/CSF2RB (hexamer)/JAK2/JAK2/SHP2 complex (CSF2RB-IL5-IL5RA-JAK2-PTPN11) (modification, collaborate)
  Pazdrak et al., J Exp Med 1997
  Evidence: physical interaction
• NCI Pathway Database IL5-mediated signaling events: GRB2 (GRB2) → IL5/IL5RA/CSF2RB (hexamer)/JAK2/JAK2/SHP2/GRB2 complex (CSF2RB-IL5-IL5RA-JAK2-PTPN11-GRB2) (modification, collaborate)
  Pazdrak et al., J Exp Med 1997
  Evidence: physical interaction
• NCI Pathway Database IL5-mediated signaling events: IL5/IL5RA/CSF2RB (hexamer)/JAK2/JAK2/SHP2 complex (CSF2RB-IL5-IL5RA-JAK2-PTPN11) → IL5/IL5RA/CSF2RB (hexamer)/JAK2/JAK2/SHP2/GRB2 complex (CSF2RB-IL5-IL5RA-JAK2-PTPN11-GRB2) (modification, collaborate)
  Pazdrak et al., J Exp Med 1997
  Evidence: physical interaction
• NCI Pathway Database IL5-mediated signaling events: IL5 (IL5) → IL5/IL5RA/JAK2 complex (IL5RA-JAK2-IL5) (modification, collaborate)
  Czabotar et al., Cytokine 2000*, Tavernier et al., Proc Natl Acad Sci U S A 1992, Murata et al., J Exp Med 1992*, Tavernier et al., Cell 1991, Devos et al., EMBO J 1991, Johanson et al., J Biol Chem 1995, Milburn et al., Nature 1993*, Takaki et al., J Exp Med 1993*, Li et al., Proc Natl Acad Sci U S A 1997*, Ogata et al., Blood 1998
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL5-mediated signaling events: IL5 (IL5) → IL5RA/JAK2 complex (IL5RA-JAK2) (modification, collaborate)
  Czabotar et al., Cytokine 2000*, Tavernier et al., Proc Natl Acad Sci U S A 1992, Murata et al., J Exp Med 1992*, Tavernier et al., Cell 1991, Devos et al., EMBO J 1991, Johanson et al., J Biol Chem 1995, Milburn et al., Nature 1993*, Takaki et al., J Exp Med 1993*, Li et al., Proc Natl Acad Sci U S A 1997*, Ogata et al., Blood 1998
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL5-mediated signaling events: IL5/IL5RA/JAK2 complex (IL5RA-JAK2-IL5) → IL5RA/JAK2 complex (IL5RA-JAK2) (modification, collaborate)
  Czabotar et al., Cytokine 2000*, Tavernier et al., Proc Natl Acad Sci U S A 1992, Murata et al., J Exp Med 1992*, Tavernier et al., Cell 1991, Devos et al., EMBO J 1991, Johanson et al., J Biol Chem 1995, Milburn et al., Nature 1993*, Takaki et al., J Exp Med 1993*, Li et al., Proc Natl Acad Sci U S A 1997*, Ogata et al., Blood 1998
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL5-mediated signaling events: IL5/IL5RA complex (IL5RA-IL5) → IL5/IL5RA/CSF2RB (hexamer) complex (CSF2RB-IL5-IL5RA) (modification, collaborate)
  Sun et al., Blood 1999, Mulhern et al., J Mol Biol 2000, Rossjohn et al., Blood 2000, Carr et al., Cell 2001, Carr et al., Acta crystallographica. Section F, Structural biology and crystallization communications 2006, Tavernier et al., Cell 1991, Cornelis et al., Eur J Immunol 1995*, Johanson et al., J Biol Chem 1995, Woodcock et al., EMBO J 1994
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL5-mediated signaling events: IL5/IL5RA complex (IL5RA-IL5) → CSF2RB (dimer) complex (CSF2RB) (modification, collaborate)
  Sun et al., Blood 1999, Mulhern et al., J Mol Biol 2000, Rossjohn et al., Blood 2000, Carr et al., Cell 2001, Carr et al., Acta crystallographica. Section F, Structural biology and crystallization communications 2006, Tavernier et al., Cell 1991, Cornelis et al., Eur J Immunol 1995*, Johanson et al., J Biol Chem 1995, Woodcock et al., EMBO J 1994
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL5-mediated signaling events: IL5/IL5RA/CSF2RB (hexamer) complex (CSF2RB-IL5-IL5RA) → CSF2RB (dimer) complex (CSF2RB) (modification, collaborate)
  Sun et al., Blood 1999, Mulhern et al., J Mol Biol 2000, Rossjohn et al., Blood 2000, Carr et al., Cell 2001, Carr et al., Acta crystallographica. Section F, Structural biology and crystallization communications 2006, Tavernier et al., Cell 1991, Cornelis et al., Eur J Immunol 1995*, Johanson et al., J Biol Chem 1995, Woodcock et al., EMBO J 1994
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL5-mediated signaling events: IL5/IL5RA/CSF2RB (hexamer)/JAK2/JAK2 complex (CSF2RB-IL5-IL5RA-JAK2) → PI3K complex (PIK3CA-PIK3R1) (modification, activates)
  Dijkers et al., Oncogene 1999
  Evidence: mutant phenotype, assay
• NCI Pathway Database IL5-mediated signaling events: IL5/IL5RA/JAK2 complex (IL5RA-JAK2-IL5) → IL5/IL5RA/CSF2RB (hexamer)/JAK2/JAK2 complex (CSF2RB-IL5-IL5RA-JAK2) (modification, collaborate)
  Sun et al., Blood 1999, Mulhern et al., J Mol Biol 2000, Rossjohn et al., Blood 2000, Carr et al., Cell 2001, Carr et al., Acta crystallographica. Section F, Structural biology and crystallization communications 2006, Martinez-Moczygemba et al., J Leukoc Biol 2007*, Tavernier et al., Cell 1991, Lei et al., J Leukoc Biol 2008*, Sato et al., J Exp Med 1994, Cornelis et al., Eur J Immunol 1995*, Johanson et al., J Biol Chem 1995, Woodcock et al., EMBO J 1994, Kouro et al., Int Immunol 1996, Ogata et al., Blood 1998
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL5-mediated signaling events: IL5/IL5RA/JAK2 complex (IL5RA-JAK2-IL5) → CSF2RB (dimer) complex (CSF2RB) (modification, collaborate)
  Sun et al., Blood 1999, Mulhern et al., J Mol Biol 2000, Rossjohn et al., Blood 2000, Carr et al., Cell 2001, Carr et al., Acta crystallographica. Section F, Structural biology and crystallization communications 2006, Martinez-Moczygemba et al., J Leukoc Biol 2007*, Tavernier et al., Cell 1991, Lei et al., J Leukoc Biol 2008*, Sato et al., J Exp Med 1994, Cornelis et al., Eur J Immunol 1995*, Johanson et al., J Biol Chem 1995, Woodcock et al., EMBO J 1994, Kouro et al., Int Immunol 1996, Ogata et al., Blood 1998
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL5-mediated signaling events: IL5/IL5RA/CSF2RB (hexamer)/JAK2/JAK2 complex (CSF2RB-IL5-IL5RA-JAK2) → CSF2RB (dimer) complex (CSF2RB) (modification, collaborate)
  Sun et al., Blood 1999, Mulhern et al., J Mol Biol 2000, Rossjohn et al., Blood 2000, Carr et al., Cell 2001, Carr et al., Acta crystallographica. Section F, Structural biology and crystallization communications 2006, Martinez-Moczygemba et al., J Leukoc Biol 2007*, Tavernier et al., Cell 1991, Lei et al., J Leukoc Biol 2008*, Sato et al., J Exp Med 1994, Cornelis et al., Eur J Immunol 1995*, Johanson et al., J Biol Chem 1995, Woodcock et al., EMBO J 1994, Kouro et al., Int Immunol 1996, Ogata et al., Blood 1998
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL5-mediated signaling events: IL5/IL5RA/CSF2RB (hexamer)/JAK2/JAK2 complex (CSF2RB-IL5-IL5RA-JAK2) → SHP2 (PTPN11) (modification, collaborate) Pazdrak et al., J Exp Med 1997
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL5-mediated signaling events: IL5/IL5RA/CSF2RB (hexamer)/JAK2/JAK2 complex (CSF2RB-IL5-IL5RA-JAK2) → IL5/IL5RA/CSF2RB (hexamer)/JAK2/JAK2/SHP2 complex (CSF2RB-IL5-IL5RA-JAK2-PTPN11) (modification, collaborate)
  Pazdrak et al., J Exp Med 1997
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL5-mediated signaling events: SHP2 (PTPN11) → IL5/IL5RA/CSF2RB (hexamer)/JAK2/JAK2/SHP2 complex (CSF2RB-IL5-IL5RA-JAK2-PTPN11) (modification, collaborate)
  Pazdrak et al., J Exp Med 1997
  Evidence: mutant phenotype, assay, physical interaction
• Reactome Reaction: IL5RA → IL5 (reaction) Sakamaki et al., EMBO J 1992, Duronio et al., J Biol Chem 1992, Tavernier et al., Cell 1991, Sorensen et al., J Biol Chem 1989, Quelle et al., Mol Cell Biol 1994, Feng et al., Mol Cell Biol 1997, Bone et al., J Biol Chem 1997, Flores-Morales et al., Mol Cell Endocrinol 1998
• Reactome Reaction: IL5RA → IL5 (indirect_complex) Tavernier et al., Cell 1991

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

• IRef Bind_translation Interaction: IL5 — IL5RA (electrophoretic mobility shift assay) Tavernier et al., Proc Natl Acad Sci U S A 1992
• IRef Biogrid Interaction: IL5 — IL5RA (direct interaction, pull down) Woodcock et al., EMBO J 1994
• IRef Biogrid Interaction: IL5 — IL5RA (colocalization, biochemical) Johanson et al., J Biol Chem 1995
• IRef Biogrid Interaction: IL5 — IL5RA (direct interaction, pull down) Johanson et al., J Biol Chem 1995
• IRef Biogrid Interaction: IL5 — IL5RA (direct interaction, pull down) Murata et al., J Exp Med 1992*
• IRef Dip Interaction: IL5 — IL5RA (direct interaction, molecular sieving) Patino et al., Structure 2011*
• IRef Dip Interaction: IL5 — IL5RA (direct interaction, cross-linking study) Tavernier et al., Proc Natl Acad Sci U S A 1992
• IRef Dip Interaction: IL5 — IL5RA (direct interaction, x-ray crystallography) Patino et al., Structure 2011*
• IRef Dip Interaction: IL5 — IL5RA (direct interaction, surface plasmon resonance) Patino et al., Structure 2011*
• IRef Hprd Interaction: Complex of 19 proteins (in vivo) Plugariu et al., Biochemistry 2000*, Scibek et al., Anal Biochem 2002*
• IRef Hprd Interaction: IL5 — IL5RA (in vivo) Czabotar et al., Cytokine 2000*, Murata et al., J Exp Med 1992*, Johanson et al., J Biol Chem 1995
• IRef Hprd Interaction: IL5 — IL5RA (in vitro) Czabotar et al., Cytokine 2000*, Murata et al., J Exp Med 1992*, Johanson et al., J Biol Chem 1995
• IRef Innatedb Interaction: IL5 — IL5RA (unknown, -) Murata et al., J Exp Med 1992*
• IRef Innatedb Interaction: IL5 — IL5RA (unknown, -) Johanson et al., J Biol Chem 1995
• IRef Intact Interaction: Complex of IL5RA-CSF2RB-IL5-CSF2RB-IL5-IL5RA (physical association, saturation binding) Tavernier et al., Proc Natl Acad Sci U S A 1992
• IRef Intact Interaction: IL5 — IL5RA (physical association, saturation binding) Tavernier et al., Proc Natl Acad Sci U S A 1992
• IRef Intact Interaction: IL5 — IL5RA (physical association, cross-linking study) Tavernier et al., Proc Natl Acad Sci U S A 1992
• IRef Ophid Interaction: IL5 — IL5RA (aggregation, interologs mapping) Brown et al., Bioinformatics 2005
• IRef Ophid Interaction: IL5 — IL5RA (aggregation, confirmational text mining) Czabotar et al., Cytokine 2000*

Text-mined interactions from Literome

Denburg et al., Int Arch Allergy Immunol 2001 (Asthma...) : Little is currently known concerning regulation of IL-5Ralpha gene transcription in the context of commitment of hemopoietic progenitor cells to the eosinophil and basophil lineages ; recent studies have indicated that IL-5 itself can regulate IL-5Ralpha expression on mature eosinophils
Stafford et al., J Immunol 2002 : We have demonstrated that IL-5 induces tyrosine phosphorylation of IL-5Ralpha in eosinophils
Hellman et al., Clin Exp Immunol 2003 (Eosinophilia) : The IL-5R alpha was down-regulated , in a dose dependent manner, by recombinant IL-5 and GM-CSF, with IL-5 being most potent
Sergejeva et al., Blood 2004 : We further demonstrate in vitro that ability to multiply persists in BALf CD34 ( + ) cells but not CD34 ( - ) cells, likely via autocrine IL-5 release and IL-5 induced up-regulation of IL-5R alpha
Hamaguchi-Tsuru et al., Br J Haematol 2004 : In contrast, C/EBPepsilon, IL-5Ralpha , and eosinophil peroxidase mRNAs were induced in response to IL-3 and IL-5 after transfer onto OP9 cells
Verma et al., Blood 2009 : Those from hosts given IL-4 cultured cells had enhanced IL-5Ralpha mRNA expression and IL-5 enhanced their proliferation to donor but not third-party allo-Ag
Takagi et al., J Exp Med 1995 : These results strongly suggest that the lineage specificity of IL-5 is mainly due to the restricted expression of IL-5R alpha
Mita et al., J Immunol 1993 : To characterize the roles of IL-5R alpha and IL-5R beta on the association, dissociation, and internalization of IL-5 , we compared the binding kinetics and the internalization of 35S labeled IL-5 by high affinity IL-5R ( dissociation constant approximately 150 pM ) bearing T88-M with those by low affinity IL-5R ( dissociation constant approximately 30 nM ) bearing MOPC104E
Shiiba et al., Int Arch Allergy Immunol 1996 (Leukemia, B-Cell) : These results support the notion that the lineage specificity of IL-5 is mainly due to the restricted expression of IL-5R alpha