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IFNG — RELA

Pathways - manually collected, often from reviews:

• BioCarta the 41bb-dependent immune response: IFN-gamma (IFNG) → NF-kappa-B complex (RELA) (transcription, collaborate)

Text-mined interactions from Literome

Nomura et al., Toxicol Lett 1998 (Ischemic Attack, Transient) : LPS caused intranuclear translocation of NF-kappaB , and IFNgamma induced phosphorylation of Jak2 and Stat1, followed by the translocation of Stat1 into the nucleus
Novick et al., Immunity 1999 : IL-18BP abolished IL-18 induction of interferon-gamma (IFNgamma) , IL-8, and activation of NF-kappaB in vitro
Ouaaz et al., J Exp Med 1999 : Our results indicate not only that RelA is required for activation of key genes involved in adaptive ( acquired ) immune responses, including major histocompatibility complex class I, CD40, and the Fas death receptor, but also that both NF-kappaB inducing signals and IFN-gamma are necessary for maximal activation
Nakashima et al., J Am Soc Nephrol 1999 : Cells were stimulated with TNF-alpha or LPS plus IFN-gamma in the presence of NF-kappaB inhibitors, herbimycin A (HerA), or the more specific JAK2 inhibitor AG490
Kojima et al., J Immunol 1999 : An essential role for NF-kappa B in IL-18 induced IFN-gamma expression in KG-1 cells ... In addition, stable transformants of a dominant negative form of the I kappa B alpha showed an inhibition of IL-18 dependent I kappa B alpha degradation, NF-kappa B activation , and expression of IFN-gamma
Seppänen et al., Oncol Res 1998 (Adenocarcinoma...) : In the present study, we have investigated the effects of interferons-alpha (IFN-alpha) and -gamma ( IFN-gamma ), interleukin-10 (IL-10) and -13 ( IL-13 ), transforming growth factor-beta1 ( TGF-beta1 ), granulocyte-macrophage colony stimulating factor ( GM-CSF ), and tumor necrosis factor-alpha (TNF-alpha) on cell proliferation and induction of transcription factors AP-1 and NF-kappaB in UM-EC-3 human endometrial adenocarcinoma cells and UT-OC-5 ovarian carcinoma cells in vitro
Tsuji-Takayama et al., Cell Immunol 1999 : Interleukin-18 induces interferon-gamma production through NF-kappaB and NFAT activation in murine T helper type 1 cells
Wang et al., Br J Pharmacol 1999 (Brain Neoplasms...) : Results showed that nepalolide A diminished the LPS/IFN-gamma mediated association of NF-kappaB with consensus oligonucleotide in a concentration dependent manner
Mizutani et al., J Nutr Sci Vitaminol (Tokyo) 1999 : Ascorbate could modulate NF-kappa B DNA binding activity in response to combined LPS and IFN-gamma activation, which increases NO production in activated macrophages
Lee et al., J Immunol 2000 : STAT-1alpha and NF-kappaB activation are involved in IFN-gamma- or TNF-alpha mediated Fas up-regulation in microglia, respectively
Hamada et al., Toxicology 2000 : However, NF-kappaB was increased at 3 h while AP-1 ( Jun B and Jun D ) and CREB were increased at 15 h. Co-stimulation of 12j cells with rMu-IFN-gamma + Be increased the levels of NF-KB in 12j cell nuclei at 3 h, and the levels of AP-1 and CREB at 15 h, however, only Jun B was increased
Smith et al., J Gen Virol 2000 : The ectromelia virus protein was found to block NF-kappaB activation and induction of IFN-gamma in response to IL-18
Sekine et al., J Cell Physiol 2000 : Synergistic activation of NF-kappab and inducible isoform of nitric oxide synthase induction by interferon-gamma and tumor necrosis factor-alpha in INS-1 cells
Gupta et al., Kidney Int 2000 : To test the hypothesis that alpha-MSH inhibits the expression of inducible trans activating factors involved in NOS2 regulation, we used RAW 264.7 macrophage cells to examine the effects of alpha-MSH on the activation of nuclear factor-kappaB (NF-kappaB) and CCAAT/enhancer binding protein-beta ( C/EBPbeta ), trans acting factors known to be involved in LPS + interferon (IFN)-gamma induction of the NOS2 gene
Ryu et al., Biochem Biophys Res Commun 2000 : Acetaminophen inhibited iNOS mRNA expression, promoter activity of iNOS gene and nuclear factor-kappa B (NF-kappaB) binding activity induced by LPS plus IFN-gamma , whereas salicylates did not show any effect on them
Possel et al., Glia 2000 : By contrast, LPS and interferon-gamma led to translocation of NF-kappaB in microglia and in astrocytes, demonstrating that both cell types are responsive to the stimulus
Faure et al., J Immunol 2001 (Inflammation...) : Furthermore, LPS and IFN-gamma act synergistically to induce TLR2 expression in EC, and LPS induced TLR2 expression is NF-kappaB dependent
Mandrika et al., Biochem Pharmacol 2001 : Effects of melanocortin peptides on lipopolysaccharide/interferon-gamma induced NF-kappaB DNA binding and nitric oxide production in macrophage-like RAW 264.7 cells : evidence for dual mechanisms of action
Tsukagoshi et al., Regul Pept 2001 : ANP inhibited the IFN-gamma induced p38 MAP kinase activation, and ANP and SB203580 inhibited NF-kappaB activation
Nomura et al., Life Sci 2001 : Experiments using stable clones of rat C6 glioma cells transfected with dominant negative IkappaB alpha ( serines 32 and 36 replaced by alanine ) suggest that NF-kappaB activation ( phosphorylation of IkappaB alpha ) is involved in LPS/IFNgamma- or IL-1beta/IFNgamma induced iNOS expression
Suk et al., J Biol Chem 2001 (Necrosis...) : IFNgamma inhibits cytoprotective NF-kappa B through STAT1/IRF-1 pathways
Steiner et al., Eur Urol 2001 (Carcinoma, Renal Cell) : NF-kB signaling induced by TNFalpha is suppressed by IFNalpha and IFNgamma in the permanent cell lines and in the primary RCC tumor cell cultures
Deb et al., J Immunol 2001 : RNA dependent protein kinase PKR is required for activation of NF-kappa B by IFN-gamma in a STAT1 independent pathway ... Here we show that IFN-gamma alone can activate NF-kappaB , by a Janus kinase-1 mediated, but Stat1 independent, mechanism ... NF-kappaB activation by IFN-gamma is associated with degradation of IkappaB beta
Suk et al., Immunol Lett 2001 : While IFNgamma enhanced LPS induced NF-kB activation, PGE ( 2 ) suppressed its activation
Crane et al., Cell Immunol 2001 (Calcium Signaling) : The results show that IFN-gamma down-regulation acts either directly on NF-kappaB or its activation or by blockade of a pathway upstream of NF-kappaB
Roth et al., Biochim Biophys Acta 2001 : Neither was NF-kappa B-independent activation of endothelial cells by interferon-gamma influenced by homocysteine
Jana et al., J Biol Chem 2001 : Stimulation of IFN-gamma mediated activation of NF-kappaB but not of C/EBPbeta by CD40 ligation suggests that CD40 ligation stimulates the expression of iNOS in IFN-gamma treated BV-2 microglial cells through the stimulation of NF-kappaB activation
Qin et al., J Invest Dermatol 2001 : These results indicate that interferon-gamma and TNF-alpha synergistically induce keratinocyte apoptosis when concomitant induction of NF-kappaB is blocked
Teng et al., Am J Physiol Cell Physiol 2002 : IL-1 beta, but not IFN-gamma , induced nuclear factor (NF)-kappa B and CCAAT box/enhancer binding protein ( C/EBP ) nuclear binding
Romagnani et al., J Am Soc Nephrol 2002 (Kidney Diseases) : Electrophoretic mobility shift assays demonstrated that NO donors repressed IP-10 gene transcription induced by IFN-gamma plus TNF-alpha through the inhibition of NF-kappaB activation
Yu et al., J Am Soc Nephrol 2002 : TSA inhibited induction of endogenous NO production and iNOS as well as NF-kappa B element promoter activity in response to interleukin-1 beta (IL-1 beta) or lipopolysaccharide (LPS) + interferon-gamma (IFN-gamma) in both cell types without altering NF-kappa B DNA binding activity
Wesemann et al., J Immunol 2002 : IFN-gamma leads to STAT-1alpha activation directly and up-regulation of NF-kappaB activity due to the secretion and subsequent autocrine signaling of TNF-alpha ... Additionally, IFN-gamma induced TNF-alpha secretion, as well as STAT-1alpha and NF-kappaB activation , are inhibited in the presence of SOCS-1
Badgwell et al., Surgery 2002 (Escherichia coli Infections...) : The 2 groups of mice were analyzed for serum levels of interferon-gamma , tumor necrosis factor-alpha, and interleukin-1beta as well as activation of NFkappaB and STAT1, 2 proinflammatory transcription factors
Pajonk et al., Cancer Res 2002 (Prostatic Neoplasms) : The effect of saquinavir on LPS/IFN-gamma induced activation of NF-kappaB was assessed by gel-shift assays and by Western analysis of corresponding IkappaBalpha-levels ... Saquinavir treatment prevented LPS/IFN-gamma induced activation of NF-kappaB in RAW cells and stabilized expression of IkappaBalpha
Maruyama et al., J Immunol 2003 : The induction of the IL-12 p40 gene is regulated by NF-kappaB in the presence of IFN-gamma
Artis et al., J Immunol 2003 (Leishmaniasis, Cutaneous) : Critically, the frequency of proliferating KO CD4 ( + ) T cells secreting IFN-gamma matched that of wild-type cells, suggesting that NF-kappaB1 was not required for efficient transcription of the IFN-gamma gene
Takao et al., Br J Dermatol 2003 : Phorbol myristate acetate ( PMA ), tumour necrosis factor alpha, and interferon gamma each activated NF-kappaB and inhibited keratinocyte proliferation
Chae et al., Res Commun Mol Pathol Pharmacol 2001 : However, a specific inhibitor of p38 MAPK, SB203580, had no effect on TNF-alpha/IFN-gamma or LPS/IFN-gamma induced NF-kappaB activation
Fu et al., Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 2003 : Our results showed that the two mutants induced significantly less amount of IFN-gamma from PBMC ( 13 %, 48 % of wild type rhIL-18 for Delta NC, S, respectively ), and the activation of NF-kappa B also lower than wild type rhIL-18 ( 69.7 %, 89.8 % of wild type rhIL-18 respectively ), indicating that the deleted or mutated amino acids might be important for IL-18 function
Beppu et al., Biochem Biophys Res Commun 2003 (Carcinoma...) : These findings suggest the existence of IFN-gamma induced anti-apoptotic pathway and we evaluated the effect of IFN-gamma and CsA on calcium-sensitive nuclear factor-kappa B (NF-kappa B) activation ... Activation of NF-kappa B occurred in response to IFN-gamma , and which was markedly inhibited by either CsA or FK506 ... These results suggest that IFN-gamma may simultaneously induce the STAT1 mediated apoptotic pathway and the anti-apoptotic pathway through calcium activated NF-kappa B and that inhibition of the latter by CsA may result in dominance of the apoptosis inducing pathway
Huang et al., J Biomed Sci 2003 : We found that LPS induced I kappa B kinase and nuclear factor-kappa B (NF-kappa B) activation , as well as IFN-gamma induced signal transducer and activator of transcription 1 ( STAT1 ) phosphorylation, were reduced by lovastatin
Janicke et al., Res Vet Sci 2003 : LPS and IFNgamma caused translocation of the transcription factor nuclear factor kappa B (NfkappaB) to the nucleus in equine cells suggesting the limited iNOS production seen in our cells is not due to deficits in this signal transduction pathway
Corn et al., J Immunol 2003 : The role of NF-kappaB in IFN-gamma gene expression includes a modest decrease in Stat4 activation, T box expressed in T cell levels, and differentiation efficiency along with a more prominent postdifferentiation step ... Together, these findings indicate that NF-kappaB induction in T cells regulates efficient clonal expansion, Th1 differentiation, and IFN-gamma production by Th1 lymphocytes at a control point downstream from differentiation
Han et al., J Neurosci Res 2003 : PMA and IFN-gamma synergistically enhanced activity of NF-kappaB , but not ERK
Hardaker et al., FASEB J 2004 (Inflammation...) : In contrast, IFN-gamma stimulation resulted in transient NFkappaB activation, and the inhibitor had little effect on CXCL10 expression
Kuroki et al., Cancer Immunol Immunother 2003 : Both secretion of IL-12 p70 and IFN-gamma and activation of NF-kappaB induced by OK-432 were suppressed when imMo-DCs were pretreated with cytochalasin B
Ackermann et al., Immunology 2004 : Finally, reporter gene studies with NF-kappaB enhancer elements indicated that IFN-gamma alone and IL-4 in combination with other cytokines activated NF-kappaB in HT29 cells
Arai et al., Biochem Pharmacol 2004 : In the T cells stimulated with mitogenic lectin phytohemagglutinin ( PHA ) in conjunction with phorbol myristate acetate ( PMA ), 6-formylpterin suppressed the NF-kappaB dependent transcription, the production of cytokines ( IFN-gamma and IL-2 ) and the cell proliferation
Jaramillo et al., J Immunol 2004 (MAP Kinase Signaling System) : MSU crystals exerted their up-regulating effect by increasing extracellular signal regulated kinase ( ERK ) 1/2 phosphorylation and NF-kappa B nuclear translocation in response to IFN-gamma
Chae et al., Immunopharmacol Immunotoxicol 2004 : TNF-alpha plus IFN-gamma can induce extracellular signal regulated kinase ( ERK ), IKK, IkappaB degradation and NF-kappaB activation
Hwang et al., Biochem Biophys Res Commun 2004 : The inhibitory effect of LY294002 on NO generation may be mediated through specific inhibition of signal transducer and activator-1 ( STAT1 ) and NF-kappaB , which are activated by IFN-gamma
Kim et al., J Neuroimmunol 2004 : Sodium butyrate significantly inhibited NF-kappa B binding and NF-kappa B-mediated transcription induced by IFN-gamma , suggesting that the anti-inflammatory effect of sodium butyrate is mediated via specific inhibition of the NF-kappa B pathway
Casal et al., Neurosci Res 2004 (Translocation, Genetic) : The Abeta 1/42 peptide induced the activation of NF-kappaB in all studied cell cultures and its effect was potentiated by interferon-gamma (IFN-gamma) ... Abeta 25/35 alone did not induce NF-kappaB activation, but Abeta 25/35 plus IFN-gamma induced the activation of the transcription factor in the mixed and pure microglial cultures, although not in pure astroglia
Harigai et al., Arthritis Rheum 2004 (Arthritis, Rheumatoid...) : Stimulation with rsCD154 plus IFNgamma induced the activation of ERK-1/2, p38 MAPK, and NF-kappaB
Shinohara et al., Int J Mol Med 2004 : SEB and IFN-gamma activated NF-kappaB , which was inhibited by PDTC
Chang et al., J Cell Biochem 2004 : Although LIGHT could not affect IFN-gamma induced STAT1 phosphorylation and transactivation activity, which was required for the sensitization of cell death, survival NF-kappaB signaling of LIGHT was inhibited by IFN-gamma
Jia et al., Zhonghua Wai Ke Za Zhi 2004 : Low NF-kappaB activity was only detected at day 5 and day 7 in Wistar-to-Wistar group after transplantation, meanwhile low IFN-gamma mRNA expression was detected at any time in this group ... CsA down-regulates NF-kappaB activity and further inhibit IFN-gamma gene transcription
Shiao et al., Br J Pharmacol 2005 : Treatment with 50 microM of falcarindiol for 30 min decreased LPS/IFN-gamma induced nuclear factor-kappaB (NF-kappaB) activation by 32 %
Jana et al., Free Radic Biol Med 2005 : All three cytokines alone induced the activation of AP-1 while IL-1beta and TNF-alpha but not IFN-gamma induced the activation of NF-kappaB
Li et al., Cancer Res 2005 (Fibrosarcoma) : Furthermore, a specific NF-kappaB inhibitor significantly suppressed IL-12/IL-18 induced IFNgamma secretion, thus confirming the requirement for NF-kappaB activation in IL-12/IL-18 signaling
Rodriguez-Galán et al., J Immunol 2005 : The IL-2-plus-IL-18 or IL-12-plus-IL-18 effect on IFN-gamma and IL-13 expression is dependent on Stat4 and NF-kappaB but independent of Stat6, T-bet, or NFAT
Sae-Ung et al., Eur J Haematol 2005 : In contrast, NF-kappaB was undetectable in the nucleus in the presence of IFN-gamma
Park et al., Gastroenterology 2005 (Carcinoma, Hepatocellular...) : Interferon-gamma inhibits hepatitis B virus induced NF-kappaB activation through nuclear localization of NF-kappaB inducing kinase ... However, interferon (IFN)-gamma induced NIK nuclear localization and inhibited NF-kappaB activation in HepG2.2.15 cells and in HepG2 cells transfected with pHBV1.2x ... When NIK nuclear localization was inhibited by deletion of nuclear localization signal on NIK, IFN-gamma did not induce the NIK nuclear localization and did not inhibit NF-kappaB activation ... IFN-gamma selectively inhibits HBV mediated NF-kappaB activation
Wu et al., Transplant Proc 2005 : On posttransplant day 7, the serum albumin level significantly improved, the serum total bile acid and alanine aminotransferase levels were significantly lower, the serum interleukin-2 and interferon-gamma levels were significantly lower, and the activation of transcription factor NF-kappaB in peripheral blood T lymphocytes was significantly suppressed in the combination group in comparison with those in the CsA group ( all P < .05 ) or control group ( all P < .01 ), and a histological examination revealed apparent difference in the severity of rejection between the combination group and CsA group ( P < .05 ) or control group ( P < .01 )
Ko et al., Planta Med 2005 : Similarly, a time related NF-kappaB/p65 nuclear translocation induced by LPS/IFN-gamma was diminished in the presence of laxifolone A ... In summary, these results suggest that inhibition of NF-kappaB p105 degradation in cytoplasm may participate in the abrogation of LPS/IFN-gamma induced NF-kappaB translocation and subsequent NO synthesis by laxifolone A
Yamada et al., J Urol 2005 (Urinary Bladder Neoplasms) : Although LPS and IFN-gamma alone marginally activated nuclear factor (NF)-kappaB but not AP-1, LPS plus IFN-gamma augmented NF-kappaB and AP-1
Hasel et al., Lab Invest 2005 (Fibrosis...) : RelA transcriptional activity increased upon stimulation with IFNgamma and IFNgamma/TRAIL
Chen et al., Eur J Pharmacol 2005 : LPS induced IkappaB kinase (IKK), nuclear factor-kappaB (NF-kappaB) and activating protein-1 (AP-1) activation, and IFN-gamma induced NF-kappaB , signal transducer and activator of transcription-1 ( STAT1 ) and interferon regulatory factor-1 (IRF-1) activation were reduced by quercetin
Sheu et al., Food Chem Toxicol 2006 (Glomerulonephritis) : Electrophoretic mobility shift assay experiments demonstrated that CLA ( 100 microM ) dramatically reduced activation of nuclear factor-kappaB (NF-kappaB) , activator protein-1 (AP-1) and cAMP response element binding protein ( CREB ) induced by LPS/IFN-gamma
Jensen et al., J Endocrinol 2005 : Gel retardation experiments showed that hGH affects neither IFN-gamma+TNF-alpha induced STAT1 DNA binding nor IL-1beta and IFN-gamma+TNF-alpha induced NFkappaB DNA binding
Narumi et al., J Immunol 1992 : IFN-gamma and IL-2 cooperatively activate NF kappa B in murine peritoneal macrophages ... Although the effects of LPS and IFN-gamma/IL-2 on NF kappa B activity were both transient, response to LPS was detected earlier and declined more rapidly than that seen with IFN-gamma/IL-2 treatment ... The activation of NF kappa B by IFN-gamma/IL-2 did not depend upon protein synthesis
Ganster et al., J Interferon Cytokine Res 2005 (Inflammation) : In contrast, IFN-gamma inhibits TNF-alpha induced transcription of an NF-kappaB reporter gene in a Stat1 dependent mechanism in 2fTGH fibroblasts
Maksimow et al., J Leukoc Biol 2006 : Inhibition of NF-kappaB simultaneously with Fas ligation inhibited the increased IFN-gamma production and caused uniform death of all responding T cells
Ishiguro et al., Toxicol Appl Pharmacol 2006 (Colitis) : Furthermore, Paeonol reduced TNFalpha induced NF-kappaB transactivation and IFNgamma induced STAT1 transactivation in CW-2 cells and also in Jurkat cells
D'Alimonte et al., J Immunol 2007 : Moreover, guanosine abrogated IFN-gamma induced phosphorylation on Ser ( 727 ) and translocation of STAT-1alpha to the nucleus as well as TNF-alpha-/Abeta induced IkappaBalpha and NF-kappaB p65/RelA subunit phosphorylation, thus inhibiting NF-kappaB induced nuclear translocation ... Moreover, guanosine abrogated IFN-gamma induced phosphorylation on Ser ( 727 ) and translocation of STAT-1alpha to the nucleus as well as TNF-alpha-/Abeta induced IkappaBalpha and NF-kappaB p65/RelA subunit phosphorylation, thus inhibiting NF-kappaB induced nuclear translocation ... Guanosine effects were mediated by an increased phosphorylation of Akt, a PI3K downstream effector, as well as of ERK1/2 and p38 in the MAPK system, because culture pretreatment with selective ERK1/2, p38 MAPK, and PI3K antagonists ( U0126, SB203580, or LY294002, respectively ) counteracted guanosine inhibition on IFN-gamma/TNF-alpha induced CD40 expression and function as well as on STAT-1alpha or NF-kappaB nuclear translocation
Lee et al., J Mol Biol 2007 : In STAT1-deficient mice, nuclear factor kappaB (NF-kappaB) activation by TNF-alpha/IFN-gamma was attenuated and strongly inhibited by both NAC and L-NMMA
Hayashi et al., J Immunol 2007 (Acute Disease...) : Indeed, IFN-gamma acetylated Stat1 and reciprocally reduced NF-kappaB activation and COX-2 expression in neutrophils
Suh et al., J Virol 2007 (Cytomegalovirus Infections...) : PIC induction of IDO was mediated in part by IFN-beta but not IFN-gamma , and both NF-kappaB and interferon regulatory factor 3 (IRF3) were required
Tamassia et al., Eur J Immunol 2007 : The IFN-gamma induced STAT1 activation and the lipopolysaccharide (LPS) induced NF-kappaB activation were not enhanced if neutrophils were exposed to both stimuli, whereas both transcription factors were activated by IFN-gamma or LPS in monocytes
Jana et al., J Immunol 2007 : Involvement of NF-kappaB activation in LPS-, Abeta-, PrP-, poly IC-, Tat-, and MPP+-, but not IFN-gamma- , induced microglial expression of iNOS and stimulation of IkappaBalpha expression and inhibition of NF-kappaB activation by gemfibrozil via the PI3K pathway suggests that gemfibrozil inhibits the activation of NF-kappaB and the expression of proinflammatory molecules in microglia via PI3K mediated up-regulation of IkappaBalpha
Girart et al., J Immunol 2007 (Melanoma) : Also, secretion of IFN-gamma was dependent on MEK1/ERK, p38 MAPK, p70 ( S6 ) kinase, and NF-kappaB , but not on calcineurin
Liu et al., J Immunol 2007 : Activation of NF-kappaB , STAT6, and STAT1 was induced in eosinophils by TNF-alpha, IL-4, and IFN-gamma , respectively
Kanda et al., Am J Physiol Cell Physiol 2007 : Histamine alone enhanced transcriptional activities of NF-kappaB and activator protein-1 (AP-1) and potentiated TNF-alpha induced NF-kappaB and AP-1 activities or IFN-gamma induced NF-kappaB and STAT1 activities
Shultz et al., J Interferon Cytokine Res 2007 : Although the IFN-gamma induced expression of the IKKbeta dependent gene ip-10 is sensitive to the superrepressor ( SR ) of IkappaBalpha and requires the p65 subunit of NF-kappaB, IFN-gamma does not activate NF-kappaB ... In summary, a distinct subset of IFN-gamma dependent genes requires some components of the normal pathway of NF-kappaB activation without activation of NF-kappaB signaling by IFN-gamma
Petersson et al., BMC cancer 2007 (Breast Neoplasms) : We showed that IFN-gamma treatment and the inhibition of the transcription factor NFkappaB had a synergistic effect on psoriasin levels
Vakkila et al., Scand J Immunol 2008 : By contrast, PGE2 supplemented DC could be characterized by negligible responses to LPS and IFN-gamma and a remarkable NF-kappaB response to CD40L
Souza et al., J Endocrinol 2008 : IL-1beta, tumour necrosis factor alpha (TNFalpha) and a combination of all three pro-inflammatory cytokines increased while IFNgamma alone did not affect NFkappaB activity and iNOS gene and protein expression
Wu et al., J Cell Physiol 2008 (Sepsis) : In conclusion, LPS + IFNgamma induced NFkappaB activation is necessary for iNOS induction but is not dependent on ROS signaling
Mir et al., J Neuroimmunol 2008 : Importantly, IFN-gamma induced NF-kappa B binding to DNA was totally dependent on the endogenous TNF-alpha released via MEK/ERK signalling pathway ... Thus, exposure of BV-2 cells to IFN-gamma in the presence of the selective MEK inhibitor U0126 or a neutralizing anti-TNF-alpha antibody significantly reduced IFN-gamma dependent NF-kappa B activation and iNOs expression ... In addition, by activating the Jak/STAT pathway IFN-gamma potentiated TNF-alpha induced NF-kappa B binding to DNA and activated additional transcription factors ( i.e. IRF-1 ) known to be essential for iNOs gene expression
Seppänen et al., Acta Obstet Gynecol Scand 2008 (Carcinoma, Endometrioid...) : Activation of AP-1 seems to be involved in the growth regulating processes induced by IFN-gamma, TGF-beta(1) and TNF-alpha ; IFN-gamma is also able to increase the binding activity of NF-kappaB and inhibited apoptosis in ovarian cancer cells
Boivin et al., J Interferon Cytokine Res 2009 : IFN-gamma also caused a delayed but more prolonged activation of nuclear factor-kappaB (NF-kappaB) ; inhibition of NF-kappaB also prevented the increase in T84 TJ permeability and alteration in occludin expression ... The IFN-gamma induced activation of NF-kappaB was mediated by a cross-talk with PI3-K pathway
Pérez-Rodríguez et al., J Neurochem 2009 : IFNgamma and LPS induce nuclear factor kappaB (NFkappaB) translocation to the nucleus, a process implicated in activation of iNOS expression, as inhibition of NFkappaB translocation, by SN50, decreased iNOS expression
Sponza et al., Cell Immunol 2009 (Inflammation) : We have previously shown that IFI16 is required for proinflammatory gene stimulation by IFN-gamma through the NF-kappaB complex
Hong et al., J Surg Res 2010 : Cyclic AMP inhibits IL-1beta plus IFNgamma induced NF-kappaB translocation in hepatocytes by a PKA independent mechanism ... We previously showed that cAMP inhibits IL-1beta plus IFNgamma induced NF-kappaB binding in primary hepatocytes but the signaling mechanisms responsible for this effect are not understood ... Overexpression of PKA increased IL-1beta plus IFNgamma induced NF-kappaB binding ... We demonstrated that cAMP inhibits IL-1beta plus IFNgamma induced NF-kappaB binding due to its blockade of the upstream signal ( s ) leading to IkappaB phosphorylation and degradation, and is mediated by PKA independent signaling pathways
Ju et al., Biochem Biophys Res Commun 2009 : PGG significantly inhibited TNF-alpha/IFN-gamma induced NF-kappaB activation as well as STAT1 activation ... These results suggest that PGG may exert anti-inflammatory responses by suppressing TNF-alpha and/or IFN-gamma induced activation of NF-kappaB and STAT1 in the keratinocytes and might be a useful tool in therapy of skin inflammatory diseases
Qi et al., Br J Pharmacol 2009 : Further, inhibition of p38 mitogen activated protein kinases ( MAPKs ) significantly suppressed IFN-gamma induced MDC expression and NF-kappaB activation ... Interestingly, fluvastatin suppressed IFN-gamma induced NF-kappaB activation in parallel with p38 MAPK phosphorylation
Zhang et al., Journal of translational medicine 2009 (Diabetes Mellitus, Experimental...) : Inhibition of nuclear factor kappa B (NF-kappaB) activation induced by IL-1beta and IFN-gamma was investigated
Raices et al., PloS one 2009 : NFkappaB inhibition resulted in decreased IL-1beta/IL-18/TNFalpha stimulated IFNgamma release ... Overall, our data suggests that IkappaBzeta positively regulates NFkappaB mediated IFNgamma production in KG-1 cells
Shultz et al., J Interferon Cytokine Res 2009 : In response to IFN-gamma , inhibitor of NF-kappaB (IkappaB) kinase beta ( IKK-beta ) is required to activate both newly synthesized IRF1 and the p65 subunit of NF-kappaB, which contributes to ip-10 expression by binding to kappaB sites in the ip-10 promoter, with little or no activation of classical NF-kappaB ... IFN-gamma does not affect the transient activation of classical NF-kappaB by IL-1beta and synergistic induction of ip-10 expression by IFN-gamma and IL-1beta occurs even after the activation of classical NF-kappaB has returned to basal levels
Jung et al., Int Immunopharmacol 2010 : Inhibition of these inflammatory mediators appears to be at the transcriptional level, since diosgenin decreased LPS/IFN-gamma induced NF-kappaB and AP-1 activity
Balasubramani et al., Immunity 2010 : We find that RelA is critical for optimal Ifng expression and is differentially recruited to multiple elements contingent upon T cell receptor ( TCR ) or interleukin-12 (IL-12) plus IL-18 signaling ... STAT4 and NF-kappaB therefore cooperate at multiple cis elements to enable NF-kappaB dependent enhancement of Ifng expression
Briskin et al., Science 1988 : TGF-beta had no effect on IFN-gamma induced kappa transcription, and NF-kappa B was not activated by IFN-gamma
Kone et al., Am J Physiol 1995 : LPS + interferon-gamma (IF-gamma) promoted a time dependent increase in nitrite ( a NO metabolite ) and iNOS mRNA and the appearance of NF-kappa B p50 and p65 in nuclear protein extracts
de Wit et al., Exp Hematol 1996 : We conclude that the effects of IFN-gamma on the expression of the transcription factors AP-1 and NF-kappa B may be important for the modulatory effects of IFN-gamma on the cytokine expression in activated human monocytes
Drew et al., J Interferon Cytokine Res 1995 : We demonstrated here that TNF-alpha induced binding of NF kappa B p50 and p65 to the NF kappa B-like element of the MHC class I promoter termed region I and IFN-gamma induced binding of IRF-1 to the adjacent interferon consensus sequence ( ICS )
Abril et al., Tissue Antigens 1996 (Adenocarcinoma...) : Comparison of kappa-B binding activity showed that IFN-alpha and IFN-gamma induced opposite changes in NF-kappa B binding activity in AGS cells, indicating that the absence of MHC class I response in AGS appears to be independent of kappa-B activity
Farivar et al., J Biol Chem 1996 : Tumor necrosis factor-alpha and interferon-gamma induced NF-kappaB ( p50/p65 ) and STAT-1, respectively, as assessed by gel shift assays
Brown et al., J Interferon Cytokine Res 1997 : Interferon-gamma (IFN-gamma) , an important regulator of macrophage gene expression, significantly enhanced NF-kappa B activity and altered the apparent molecular weight of the NF-kappa B1-like proteins in LPS stimulated and IL-2 stimulated murine macrophages ... In addition, regulation of the two pools of NF-kappa B1/p50 by IFN-gamma was confirmed by Western immunoblot analysis of cytosolic and nuclear extracts
O'Brien et al., Mol Cell Biol 1997 : To address the functional role of NF-kappaB in LPS and IFN-gamma induction of these events, we blocked the nuclear translocation of NF-kappaB by overexpression of a dominant negative mutant of IkappaB-alpha ( IkappaB deltaN ) ... These results also suggest that NF-kappaB is not required for IFN-gamma activation of transcription or recombination
Cheshire et al., Mol Cell Biol 1997 : However, since activation of NF-kappaB by TNF-alpha is often transient and would not activate long-term kappaB dependent transcription effectively, we explored the effects of IFN-gamma on TNF-alpha induced NF-kappaB activity ... IFN-gamma , which typically does not activate NF-kappaB, synergistically enhanced TNF-alpha induced NF-kappaB nuclear translocation via a mechanism that involves the induced degradation of I kappaBbeta and that apparently requires tyrosine kinase activity in preneuronal cells but not in endothelial cells
Sica et al., J Biol Chem 1997 : Based on this observation, we have analyzed the potential role of NF-kappaB and NFAT family members in regulating IFN-gamma transcription
McDonald et al., Biochemistry 1998 : To circumvent this problem, we developed an alternative procedure which allowed us to show that in neutrophils, LPS and TNFalpha induce a NF-kappaB DNA binding activity which essentially consists of p50/RelA dimers, and that IFNgamma promotes the binding of STAT1 homodimers to the IFNgamma response region of the FcgammaRI promoter
Agresti et al., Eur J Neurosci 1998 : The existence of a synergism between IFN-gamma and TNF-alpha in stimulating IRF-1 expression at the transcriptional level was supported by IRF-1 promoter analysis : IFN-gamma directly induced the binding of STAT-1 homodimers to the GAS element, while NF-kappaB binding to the kappaB sequence was activated by TNF-alpha only after IFN-gamma treatment
Paludan et al., J Gen Virol 1998 (Herpes Genitalis) : HSV-2 infection and IFN-gamma stimulation of macrophages synergistically induced TNF-alpha secretion and nuclear translocation of NF-kappaB , which bound to a sequence corresponding to a kappaB site in the iNOS promoter
Held et al., Infect Immun 1999 : Consistent with the synergistic induction of inducible nitric oxide synthase mRNA and nitric oxide production by a combination of LPS and IFN-gamma, IFN-gamma strongly augmented LPS induced NF-kappaB activation and accelerated the binding of NF-kappaB to DNA to as early as 5 min
Tsubota et al., Invest Ophthalmol Vis Sci 1999 (Sjogren's Syndrome) : IFN-gamma induced the nuclear translocation of NF-kappaB , but did not activate STAT1 or STAT3 in ChWK
Wang et al., Invest Ophthalmol Vis Sci 1999 : TNF-alpha, IL-1beta, and TII but not IFN-gamma alone caused degradation of I kappaB, Rel A nuclear translocation, and increased NF-kappaB DNA binding activity, effects that were blocked by pretreatment with MG-132
Cheshire et al., J Biol Chem 1999 : The PKR inhibitor 2-aminopurine ( 2-AP ) inhibited TNF-alpha/IFN-gamma induced NF-kappaB nuclear translocation in neuronal derived cells but not in endothelial cells