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MAP2K1 — PAK1
Pathways - manually collected, often from reviews:
Protein-Protein interactions - manually collected from original source literature:
Studies that report less than 10 interactions are marked with *
Text-mined interactions from Literome
Slack-Davis et al., J Cell Biol 2003
:
We find that adhesion to fibronectin induces
PAK1 dependent phosphorylation of
MEK1 on S298 and that this phosphorylation is necessary for efficient activation of MEK1 and subsequent MAPK activation ... We propose that FAK/Src dependent,
PAK1 mediated phosphorylation of
MEK1 on S298 is central to the organization and localization of active Raf-MEK1-MAPK signaling complexes, and that formation of such complexes contributes to the adhesion dependence of growth factor signaling to MAPK ... We propose that FAK/Src dependent,
PAK1 mediated phosphorylation of
MEK1 on S298 is central to the organization and localization of active Raf-MEK1-MAPK signaling complexes, and that formation of such complexes contributes to the adhesion dependence of growth factor signaling to MAPK
Jung et al., Eur J Biochem 2004
(Melanoma) :
However, Rho associated kinase,
MAP kinase kinase 1/2 or phospholipase C might not be
involved in LPA induced
PAK1 activation or cell motility stimulation
Beeser et al., J Biol Chem 2005
:
Inhibition of
Pak kinase activity dramatically decreased phosphorylation of Mek1 at Ser ( 298 ) in response to either PDGF or EGF, but this inhibition did not
prevent Mek1 activation by EGF, suggesting that although Pak can phosphorylate Mek1 at Ser ( 298 ), this event is not required for Mek1 activation by growth factors
Park et al., Cell Signal 2007
:
MEK1 activation by
PAK : a novel mechanism ... Here we provide evidence that
PAK can directly
activate MEK1 by a mechanism distinct from conventional Ras/Raf mediated activation ... We find that
PAK phosphorylation of MEK1 serine 298
stimulates MEK1 autophosphorylation on the activation loop, and activation of
MEK1 activity towards ERK in in vitro reconstitution experiments ... Serines 218 and/or 222 in the MEK1 activation loop are required for
PAK stimulated
MEK1 activity towards ERK
Lim et al., Endocrinology 2009
:
Knockdown or inhibition of Cdc42 and
PAK1 activities also
prevented activation of
MAPK/ERK (MEK)-1/2-ERK1/2 by insulin, which was previously identified as a critical pathway for insulin regulated GLP-1 release
Lin et al., Front Biosci (Elite Ed) 2010
:
In addition, inhibition of
PAK1 resulted in abnormal localization of
mitogen activated protein kinase kinase ( MEK )
Wang et al., J Biol Chem 2013
(MAP Kinase Signaling System) :
We observed how that overexpression of a kinase-dead mutant form of
PAK1 increased phosphorylation of
MEK1/2 ( Ser-217/Ser-221 ) and ERK ( Thr-202/Tyr-204 ), although phosphorylation of B-RAF ( Ser-445 ) and C-RAF ( Ser-338 ) remained unchanged
Frost et al., EMBO J 1997
:
Expression of interfering
PAK1 also
reduces stimulation of TCF function by serum growth factors, while expression of active PAK1 enhances EGF stimulated
MEK1 activity