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CXCL12 — RAC1
Pathways - manually collected, often from reviews:
Text-mined interactions from Literome
Wright et al., J Immunol 2002
:
SDF-1alpha also
activated the small GTPases RhoA and
Rac1 , and inhibition of RhoA activation reduced the up-regulation of alpha ( 4 ) beta ( 7 ) -mediated lymphocyte adhesion in response to SDF-1alpha, suggesting that activation of RhoA could play an important role in the enhanced adhesion
Bartolomé et al., Cancer Res 2004
(Lymphatic Metastasis...) :
Moreover,
SDF-1alpha triggered the activation of the GTPases RhoA, Rac1, and Cdc42 on BLM cells, and expression of dominant negative forms of RhoA and
Rac1 , but not Cdc42, substantially
impaired the invasion of transfectants in response to SDF-1alpha, as well as the increase in MT1-MMP expression
Takesono et al., Curr Biol 2004
:
Expression of a dominant negative Itk impaired
SDF-1alpha induced migration, cell polarization, and activation of
Rac and Cdc42
García-Bernal et al., Mol Biol Cell 2005
:
CXCL12 activates the GTPase
Rac , as well as Vav1, a guanine-nucleotide exchange factor for Rac, concomitant with up-regulation of alpha4beta1 dependent adhesion ... Importantly, inhibition of Vav1 expression by RNA interference resulted in a blockade of
Rac activation in
response to
CXCL12 ... Adhesions in flow chambers and soluble binding assays using these transfectants indicated that initial ligand binding and adhesion strengthening mediated by alpha4beta1 were dependent on Vav1 and
Rac activation by
CXCL12
Friedrich et al., J Mol Cell Cardiol 2006
:
As determined by pull-down assays,
Rac1 is rapidly
activated by the chemokine
stromal derived factor-1 (SDF-1) in human monocytes ( 100 nM, 30 s )
Bartolomé et al., Cancer Res 2006
(Melanoma...) :
Blocking Vav expression by RNA interference results in impaired
activation of
Rac and Rho by
CXCL12 and in a remarkable inhibition of CXCL12 promoted invasion
Tan et al., J Biol Chem 2006
(Neoplasm Metastasis) :
We observed that whereas G ( i ) and Gbetagamma subunits are involved in
SDF-1 induced
Rac activation and cell migration, CXCR4 can also stimulate Rho potently leading to the phosphorylation of myosin light chain through the Rho effector, Rho kinase, but independently of G ( i )
Fuhler et al., Blood 2008
(MAP Kinase Signaling System...) :
Furthermore,
SDF-1 stimulated activation of
Rac and the PI3K target protein kinase B is impaired in CD34 ( + ) cells from MDS patients ... In addition, expression of constitutive active
Rac increases the motility of MDS CD34 ( + ) cells in the absence of
SDF-1 , although the directional migration of cells toward this chemoattractant is not affected
Lin et al., J Biol Chem 2011
:
In this study, we further investigated the
roles of
Rac1 , phosphoinositide 3-kinase (PI3K), and Akt in thrombin induced NF-?B activation and
IL-8/CXCL8 expression
Ghosh et al., Am J Physiol Lung Cell Mol Physiol 2012
(Acute Lung Injury) :
Treatment with AMD-3100 decreased matrix metalloproteinase-14 expression, increased tissue inhibitor of metalloproteinase-3 expression, decreased matrix metalloproteinase-2 activity, and prevented
CXCL12 induced
Rac1 activation