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NCF2 — RAC1

Pathways - manually collected, often from reviews:

• OpenBEL Selventa BEL large corpus: None → Complex of CYBA-CYBB-NCF1-NCF2-NCF4-RAC1 (increases)
  Rinckel et al., Biochem Biophys Res Commun 1999*
  Evidence: Upon stimulation, a functional NADPH oxidase is assembled when the cytosolic proteins, Rac, p67phox, p47phox, and possibly p40phox, associate with the gp91phox and p22phox transmembrane proteins.
• OpenBEL Selventa BEL large corpus: None → Complex of CYBA-CYBB-NCF1-NCF2-NCF4-RAC1 (increases, Activity)
  
  Evidence: PKC is known to induce cellular reactive oxygen species (ROS) and PKC-dependent activation of the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH oxidase has recently been shown to be responsible, in part, for increased oxidative stress in diabetes.
• BioCarta fmlp induced chemokine gene expression in hmc-1 cells: RAC1 → p67phox (NCF2) (modification, activates)
• KEGG Osteoclast differentiation: RAC1 → CYBA/CYBB/NCF1/NCF2/NCF4/NOX1/NOX3 (protein-protein, activation)
  
• KEGG Leukocyte transendothelial migration: RAC1 → Complex of CYBA-CYBB-NCF1-NCF2-NCF4-NOX1-NOX3 (protein-protein, activation)
  
• NCI Pathway Database RAC1 signaling pathway: p47 Phox/p67 Phox/cytochrome b-245 complex (NCF1-NCF2-CYBB-CYBA) → p47 Phox/p67 Phox/cytochrome b-245/RAC1/GTP complex (NCF1-NCF2-RAC1-CYBB-CYBA) (modification, collaborate)
  Mizrahi et al., J Biol Chem 2005, Heyworth et al., Mol Biol Cell 1993
  Evidence: physical interaction
• NCI Pathway Database RAC1 signaling pathway: p47 Phox/p67 Phox/cytochrome b-245 complex (NCF1-NCF2-CYBB-CYBA) → RAC1/GTP complex (RAC1) (modification, collaborate) Mizrahi et al., J Biol Chem 2005, Heyworth et al., Mol Biol Cell 1993
  Evidence: physical interaction
• NCI Pathway Database RAC1 signaling pathway: p47 Phox/p67 Phox/cytochrome b-245/RAC1/GTP complex (NCF1-NCF2-RAC1-CYBB-CYBA) → RAC1/GTP complex (RAC1) (modification, collaborate) Mizrahi et al., J Biol Chem 2005, Heyworth et al., Mol Biol Cell 1993
  Evidence: physical interaction
• Reactome Reaction: NCF2 → RAC1 (reaction) Ushio-Fukai et al., Circ Res 2002*
• Reactome Reaction: NCF2 → RAC1 (indirect_complex) Ushio-Fukai et al., Circ Res 2002*
• WikiPathways Microglia Pathogen Phagocytosis Pathway: NCF4/NCF2/NCF1 → NCF1/NCF4/NCF2/RAC1/RAC2/RAC3/CYBB/CYBA (mim-conversion)
  
• WikiPathways Microglia Pathogen Phagocytosis Pathway: RAC1/RAC2/RAC3 → NCF1/NCF4/NCF2/RAC1/RAC2/RAC3/CYBB/CYBA (activation)
  

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

• IRef Bind Interaction: RAC1 — NCF2 Lapouge et al., Mol Cell 2000*
• IRef Bind_translation Interaction: RAC1 — NCF2 (x-ray crystallography) Lapouge et al., Mol Cell 2000*
• IRef Biogrid Interaction: NCF2 — RAC1 (association, x-ray crystallography) Lapouge et al., Mol Cell 2000*
• IRef Biogrid Interaction: NCF2 — RAC1 (direct interaction, pull down) Ahmed et al., J Biol Chem 1998*
• IRef Biogrid Interaction: NCF2 — RAC1 (direct interaction, pull down) Faris et al., Biochem Biophys Res Commun 1998*
• IRef Hprd Interaction: NCF2 — RAC1 (two hybrid) Lapouge et al., Mol Cell 2000*, Gorzalczany et al., J Biol Chem 2002*, Prigmore et al., J Biol Chem 1995*, Diekmann et al., Science 1994*, Dorseuil et al., C R Seances Soc Biol Fil 1997*, Ahmed et al., J Biol Chem 1998*
• IRef Hprd Interaction: NCF2 — RAC1 (in vivo) Lapouge et al., Mol Cell 2000*, Gorzalczany et al., J Biol Chem 2002*, Prigmore et al., J Biol Chem 1995*, Diekmann et al., Science 1994*, Dorseuil et al., C R Seances Soc Biol Fil 1997*, Ahmed et al., J Biol Chem 1998*
• IRef Hprd Interaction: NCF2 — RAC1 (in vitro) Lapouge et al., Mol Cell 2000*, Gorzalczany et al., J Biol Chem 2002*, Prigmore et al., J Biol Chem 1995*, Diekmann et al., Science 1994*, Dorseuil et al., C R Seances Soc Biol Fil 1997*, Ahmed et al., J Biol Chem 1998*
• IRef Intact Interaction: RAC1 — NCF2 (physical association, isothermal titration calorimetry) Lapouge et al., Mol Cell 2000*
• IRef Intact Interaction: RAC1 — NCF2 (direct interaction, x-ray crystallography) Lapouge et al., Mol Cell 2000*
• IRef Ophid Interaction: RAC1 — NCF2 (aggregation, confirmational text mining) Dorseuil et al., C R Seances Soc Biol Fil 1997*
• IRef Ophid Interaction: RAC1 — NCF2 (aggregation, interologs mapping) Brown et al., Bioinformatics 2005

Text-mined interactions from Literome

Rinckel et al., Biochem Biophys Res Commun 1999 : Rac1 disrupts p67phox/p40phox binding : a novel role for Rac in NADPH oxidase activation
Nisimoto et al., Biochemistry 2004 : In the presence of Rac , p67N or p67phox bound to gp91C with a molar ratio of approximately 1 : 1 but neither p67N nor Rac alone showed significant binding
Robinson et al., Histochem Cell Biol 2004 : X-ray crystallography and NMR spectroscopy have provided detailed structural data on these domains and how p47-phox and p67-phox interact with p22-phox and activated Rac , respectively
Kim et al., J Leukoc Biol 2006 : Membrane translocation of p47phox and p67phox as well as Rac1 activation was not increased further by combined PMA and AA stimulation
Choi et al., Int Immunopharmacol 2006 : Translocation of p47(phox), p67(phox) and Rac was increased in response to PMA, and Tau-Cl inhibited the PMA stimulated translocation of p47(phox) and p67(phox) to plasma membrane without affecting the translocation of Rac
Miyano et al., Biochimie 2007 : We also demonstrate that, in the Nox3 based oxidase containing solely p67(phox) as supportive protein, expression of Rac1 ( Q61L ) enhances not only superoxide production but also membrane translocation of p67(phox)
Zhou et al., Free Radic Biol Med 2012 : Rac1 , a Rho-like small GTPase, enhanced p67(phox)-gp91(phox) interaction ; Rac1 inhibition decreased rotenone elicited superoxide release
Prigmore et al., J Biol Chem 1995 : A 68-kDa kinase and NADPH oxidase component p67phox are targets for Cdc42Hs and Rac1 in neutrophils ... These results suggest that the p68 kinase and p67phox are targets for Cdc42Hs and Rac1 in neutrophils
Dorseuil et al., J Biol Chem 1996 : These results indicate that activated Rac can regulate NADPH oxidase by interacting with p67phox and that Rac2 is the main p67phox interacting GTPase in human cells
Dusi et al., Biochem J 1996 : These findings indicate that, on neutrophil stimulation, p67phox mediates the translocation of p40phox and Rac1 from the cytosol to cell membranes and that Rac2 associates with the membranes independently of p47phox and p67phox