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EGF — MMP9
Text-mined interactions from Literome
Reddy et al., Int J Cancer 1999
(Breast Neoplasms...) :
Blocking these signalling pathways significantly inhibited of EGF induced cell proliferation but only partially reduced in
EGF induced
MMP-9 secretion ... In contrast, when SKBR-3 cells were exposed to MEK inhibitor ( PD 98059 ) or MAPK inhibitors ( Apigenin or MAPK antisense phosphorothioate oligodeoxynucleotides ),
EGF induced cell proliferation,
MMP-9 induction and invasion through reconstituted basement membrane were significantly reduced
Sundareshan et al., Prostate 1999
(Adenocarcinoma...) :
Induction of the
MMP , matrilysin, by
epidermal growth factor (EGF) was investigated in a human prostate cancer cell line
Huang et al., Br J Pharmacol 1999
(Neoplasm Metastasis) :
8. This study demonstrates that the inhibitory effects of Lu and Qu, and the stimulatory
effects of
EGF , on tumour cell proliferation, cellular protein phosphorylation, and
MMP secretion may be mediated at least partly through EGFR
McCawley et al., Mol Pharmacol 2000
(MAP Kinase Signaling System) :
Concentrations of forskolin that inhibit growth factor induced motility also eliminate
EGF- or scatter factor/hepatocyte growth factor dependent
induction of the
92-kDa gelatinase/MMP-9
Ellerbroek et al., Cancer Res 2001
(MAP Kinase Signaling System...) :
Inhibition of PI3K and MAPK activity impairs
EGF stimulated cell migration, in vitro invasion, and
MMP-9 production ...
EGF promotes
pro-MMP-9 binding to the cell surface through a mechanism that is independent of extracellular enzyme concentration ... Interestingly, inhibition of PI3K activity abolishes
EGF induced cell surface association of
pro-MMP-9 , whereas inhibitors of MAPKs only partially block the response
Jarrousse et al., Int J Dermatol 2001
:
Cytokine- and
EGF induced upregulation of
MMP-9 in the lower epithelial compartment of the human hair bulb is a major mechanism through which hair follicle involution, observed in alopecia, may occur
Huang et al., Int J Cancer 2002
(Carcinoma, Squamous Cell...) :
LHRH reduced both basal and
EGF stimulated secretion of
MMP-9 and MMP-2
Domeij et al., Eur J Oral Sci 2002
(MAP Kinase Signaling System) :
In addition,
MMP-1 and MMP-3 production,
induced by IL-1beta, TNFalpha or
EGF , was strongly reduced by the presence of the glucocorticoid dexamethasone
Price et al., Cell communication & adhesion 2002
(Breast Neoplasms...) :
In
response to
EGF , T4-2 cells showed a 5-fold greater secretion of
matrix metalloproteinase-9 (MMP-9) as compared to S-1 cells, and this increase was largely dependent on the activity of PI 3-kinase
Nutt et al., BJU Int 2003
(Urinary Bladder Neoplasms) :
Matrix metalloproteinases ( MMPs ) in bladder cancer : the
induction of
MMP9 by
epidermal growth factor and its detection in urine ...
MMP9 was
induced by EGF in the RT112 but not the RT4 bladder tumour cell line, whereas MMP2 production was unaffected by
EGF ...
EGF induces
MMP9 but not MMP2 in bladder cells
Bredin et al., Anticancer Res 2003
(Carcinoma, Non-Small-Cell Lung...) :
IGF I, IGF II, HGF and
EGF stimulated an enhanced expression and activity of the latent form of MMP-2 and
MMP-9
Qiu et al., Reproduction 2004
(MAP Kinase Signaling System) :
EGF induced trophoblast secretion of
MMP-9 and TIMP-1 involves activation of both PI3K and MAPK signalling pathways ... Whereas EGF up-regulates MMP-9 activity in a variety of cell types, there is no direct evidence for the
stimulation of
MMP-9 and tissue inhibitor of metalloproteinase-1 ( TIMP-1 ) secretion by
EGF in extravillous trophoblasts ... We showed for the first time that
EGF activated both PI3K/Akt and MAPK/extracellular-signal regulated kinase ( ERK ) signalling in HTR8/SVneo, and
increased both
MMP-9 and TIMP-1 mRNAs and protein concentrations ... Our results suggest that the activation of both PI3K and MAPK pathways in extravillous trophoblasts is necessary for the
up-regulation of
MMP-9 and TIMP-1 expression by
EGF
Vesey et al., Kidney Int 2005
(Calcium Signaling...) :
These responses which are dependent on its protease activity appear not to be mediated by PAR-1 activation, the autocrine action of thrombin induced TGF-beta1 secretion,
MMP activation, or
EGF receptor
transactivation
Yeudall et al., Oral Oncol 2005
(Carcinoma, Squamous Cell...) :
However,
EGF stimulated
MMP-9 activity in invasive cells
Zhang et al., Pancreas 2006
(Neoplasm Invasiveness...) :
Subsequently, the
EGF induced
MMP-9 and uPA expression and MMP-9 activity, as well as cell invasiveness, were also inhibited by these NF-kappaB inhibitors
Ahmed et al., Am J Physiol Cell Physiol 2006
(Cell Transformation, Neoplastic) :
EGF increased cell motility,
enhanced the activities of secreted
pro-matrix metalloproteinase (MMP)-2 and -9 , and enhanced expression and activation of Erk and integrin linked kinase (ILK) ... EGF withdrawal resulted in a more epithelial morphology and reversal of the
EGF induced activation of signaling pathways and
pro-MMP activity ... The inhibitors caused suppression of
EGF induced migration and
pro-MMP-2/-9 activities but did not lead to any change in EGF induced mesenchymal morphology
Rothhut et al., Biochimie 2007
(Adenoma...) :
Transient transfection and electrophoretic mobility shift assays ( EMSA ) were also performed to measure MMP-9 promoter activity, to identify multiple signaling pathways and to determine a proximal AP-1 binding site located between -79 to -73 base pairs upstream of the transcriptional start site that is involved in
activation of
MMP-9 by
EGF ... Expression of FAK related non kinase (FRNK), a potent dominant negative inhibitor of FAK, reduced FAK auto-phosphorylation and inhibited
EGF induced
MMP-9 transcription and secretion leading to decreased cell invasion through Matrigel in in vitro Transwell assays
Cowden Dahl et al., Mol Cancer Res 2007
(Neoplasm Invasiveness...) :
Additionally, inhibition of PEA3 expression via short interfering RNA reduced the
EGF induction of
MMP-9 and MMP-14 gene expression by 92 % and 50 %, respectively, and impaired EGF stimulated tumor cell invasion
Kuo et al., J Agric Food Chem 2009
(Bone Neoplasms...) :
Penta-O-galloyl-beta-D-glucose suppresses prostate cancer bone metastasis by transcriptionally repressing
EGF induced
MMP-9 expression ... 5GG inhibited the
EGF induced cell invasiveness and
MMP-9 expression in a dose- and time dependent manner by reducing the MMP-9 transcriptional activity
Kim et al., Cell Signal 2009
(Breast Neoplasms) :
EGF induced
MMP-9 expression is mediated by the JAK3/ERK pathway, but not by the JAK3/STAT-3 pathway in a SKBR3 breast cancer cell line ... In this study, we investigated the novel function of the JAK3 inhibitor, WHI-P131, on
EGF induced
MMP-9 expression and the regulatory mechanism of EGF induced MMP-9 expression in SKBR3 cells ... EGF also induced the phosphorylation of EGFR, ERK, and STAT-3, and these effects were inhibited by the EGFR inhibitor, AG1478.To investigate the involvement of the STAT-3 pathway on
EGF induced
MMP-9 expression, we pretreatedSKBR3 cells with JAK1, JAK2, and JAK3 inhibitors prior to EGF treatment ... The results showed that the JAK3 inhibitor, WHI-P131, as well as JAK3 siRNA transfection, but not the JAK1 and JAK2 inhibitors, significantly decreased
EGF induced
MMP-9 expression ... Interestingly,
EGF induced
MMP-9 expression was decreased by Ad-STAT-3 overexpression in a dose dependent manner, while it was significantly increased by STAT-3 siRNA transfection ... On the other hand,
EGF induced
MMP-9 expression was decreased by the MEK1/2 inhibitor, UO126
Yu et al., Cytotechnology 2009
:
Matrine significantly reduced the invasion,
MMP-9/MMP-2 activation , Akt phosphorylation, nuclear factor kappaB p-65 expression and DNA binding activity, and mRNA levels of MMP-9, MMP-2,
EGF and VEGFR1 in MDA-MB-231 cells
Zhao et al., Am J Pathol 2010
(Brain Neoplasms...) :
An
EGF receptor (EGFR) kinase inhibitor, a RasN17 dominant negative construct, MEK and PI3K inhibitors significantly
blocked EGF/EGFR stimulated
MMP-9 , cell invasion, and colony formation in U1242 MG cells, suggesting that MMP-9 is involved in EGFR/Ras/MEK and PI3K/AKT signaling pathway mediated cell invasion and anchorage independent growth in U1242 MG cells
Dilly et al., Mol Reprod Dev 2010
:
Epidermal growth factor (EGF) induces motility and
upregulates MMP-9 and TIMP-1 in bovine trophoblast cells ... The aim of this in vitro study was to examine
EGF mediated effects on cell motility, proliferation, and
MMP-9 and TIMP-1 expression in cultured bovine trophoblast cells
Pan et al., Invest Ophthalmol Vis Sci 2011
:
Hypertonicity induced
EGF receptor (EGFR) transactivation was
suppressed by preincubating HCECs with capsazepine, matrix metalloproteinase 1 (MMP1) inhibitor TIMP-1, broad-spectrum
MMP inhibitor GM 6001, heparin bound (HB)-EGF inhibitor CRM 197, or EGFR inhibitor AG 1478
Sen et al., Eur J Nutr 2011
(Breast Neoplasms) :
Epigallocatechin-3-gallate ( EGCG ) downregulates
EGF induced
MMP-9 in breast cancer cells : involvement of integrin receptor a5ß1 in the process ...
EGF induces
MMP-9 activity and expression ; FAK, PI3 K, and ERK are mainly involved in the process ... EGCG downregulates
EGF induced
MMP-9 expression by inhibiting the involved regulatory kinases
Blair et al., Otol Neurotol 2011
(Neoplasm Invasiveness...) :
EGF treatment moderately
induced MMP9 , but is unlikely to account for the observed invasion
Hwang et al., Mol Nutr Food Res 2011
(Fibrosarcoma...) :
Capsaicin inhibited the
epidermal growth factor (EGF) induced
activation of
matrix metalloproteinase (MMP)-9 and MMP-2, and further inhibited cell invasion and migration ... Capsaicin decreased the
EGF induced expression of MMP-9,
MMP-2 , and MT1-MMP, but did not alter TIMP-1 and TIMP-2 levels ... Furthermore, the EGFR inhibitor inhibited
EGF induced
MMP-9 expression, as well as AP-1 activity and cell migration
Arima et al., Int J Oncol 1997
:
Particularly, both the production of
MMP-9 and DNA synthesis in TE10 were
stimulated significantly by
EGF
Zhang et al., Inflammation 2012
(Arthritis, Rheumatoid...) :
IL-1ß or TNF-a increased expression of MMP-9 and MMP-2 in rheumatoid synoviocytes ;
EGF stimulated expression of
MMP-9 but not of MMP-2 ; genistein suppressed production of MMP-9 more than MMP-2 induced by IL-1ß or TNF-a ; rMMP-9, rMMP-2, or their inhibitors had no effect on the ( 3 ) H-TdR incorporation of synovial cells ... Genistein inhibited IL-1ß, TNF-a or
EGF induced proliferation and
MMP-9 expression in fibroblast-like synoviocytes of rheumatoid arthritis ; the proliferation of RA-FLS was mediated by Erk1/2 but not JNK activation, while JNK activation was involved in the signal transduction pathway leading to MMP-9 expression in rheumatoid synoviocytes
Kim et al., Cancer Lett 2012
(Breast Neoplasms) :
We determined the effect of smad7 on
EGF induced
MMP-9 expression in SKBR3 breast cancer cells ... The expression of smad7 and
MMP-9 was
increased by
EGF or TGF-ß1, respectively, and further increased by EGF and TGF-ß1 co-treatment ... In addition,
EGF induced
MMP-9 expression was inhibited by UO126 ( a MEK1/2 inhibitor ) or SIS3 ( a smad3 inhibitor ), but not by SP600125 ( a JNK inhibitor ) ... We also investigated the role of smad3 on
EGF induced
MMP-9 expression and showed that EGF induced MMP-9 expression was decreased by smad3 siRNA transfection, whereas EGF induced MMP-9 expression was further increased by smad3 over-expression, as expected
Tsai et al., Toxicon 2012
(MAP Kinase Signaling System...) :
CTX III inhibited the
EGF induced activation of
matrix metalloproteinase-9 (MMP-9) , and further suppressed cell invasion and migration without obvious cellular cytotoxicity
Park et al., Br J Nutr 2013
(Neoplasm Invasiveness...) :
Maslinic acid significantly ( P < 0·05 ) down-regulated both basal and
EGF stimulated secretion of matrix metalloproteinase
(MMP)-9 ( 25-67 % ), MMP-2 ( 50-86 % ), urokinase-type plasminogen activator ( uPA, about 100 % ), vascular endothelial growth factor ( VEGF, 98-100 % ) and tissue inhibitors of metalloproteinases ( TIMP ) -1, as well as expression of uPA receptor (uPAR), intercellular adhesion molecules ( 22-33 % ), vascular cell adhesion molecules ( 23-46 % ) and E-cadherin, whereas it increased TIMP-2 secretion
Hsieh et al., Toxicol In Vitro 2013
(Breast Neoplasms...) :
Furthermore, the EGFR inhibitor AG1478 inhibited
EGF induced
MMP-9 expression, cell migration and invasion, as well as the activation of PI3K/Akt, suggesting that PI3K/Akt activation occur downstream of EGFR activation
Harvey et al., Development 1995
:
Both LIF and
EGF stimulated uPA and
MMP-9 activity in blastocyst outgrowths after 3 days of culture ( day 7 )
Harvey et al., Hum Reprod 1995
:
Recent experiments show that both LIF and
EGF stimulate secretion of urokinase-type plasminogen activator ( uPA ) and
gelatinase B/matrix metalloproteinase-9 (MMP-9) in day 7 mouse blastocyst outgrowths
Chen et al., Biochem Biophys Res Commun 1993
:
Our results showed that
EGF induced 92 kDa
MMP-9 secretion was doubled in the ras transformed keratinocytes in comparison to the parent cells
Shimizu et al., Cancer Res 1996
(Carcinoma...) :
Secretion of
gelatinase B by LuM1 cells was
augmented by tumor necrosis factor alpha, transforming growth factor beta1 ( TGF-beta1 ), interleukin 1beta, or
epidermal growth factor , and specific neutralizing antibodies abolished the induced increases
Ueda et al., Int J Cancer 1997
(Carcinoma, Squamous Cell...) :
We investigated the
effects of
epidermal growth factor (EGF) and transforming growth factor (TGF)-alpha on migration, invasion and
matrix metalloproteinase ( MMP ) expression of uterine cervical-carcinoma SKG-IIIb cells, and whether these growth factors affect pyrimidine-nucleoside-phosphorylase ( PyNPase ) activity and 5'-deoxy-5-fluorouridine ( 5'-dFUrd ) sensitivity of tumor cells
Festuccia et al., Int J Cancer 1998
(Neoplasm Invasiveness...) :
Similarly to
EGF , bombesin stimulates secretion and
activation of
MMP-9 and TIMP-1 production
Watabe et al., Int J Cancer 1998
(Breast Neoplasms...) :
Here, we have identified transcriptional requirements for the
induction of uPA and
92 kDa type IV collagenase by
epidermal growth factor (EGF) ... Reporter transfection assays revealed the
activation of uPA and
MMP-9 collagenase promoters by
EGF and the requirement of each of the composite Ets and AP-1 transcription factor binding sites for an EGF response ... Most notably, transfections with the Ets-1 and Ets-2 expression vectors potentiated uPA and
MMP-9 promoter activation in
response to
EGF
Charvat et al., Exp Dermatol 1998
:
Cell migration and
MMP-9 secretion are
increased by
epidermal growth factor in HaCaT-ras transfected cells
Ellerbroek et al., Int J Cancer 1998
(Neoplasm Invasiveness...) :
EGF dependent stimulation of migration and induction of
matrix metalloproteinase (MMP)-9 ( gelatinase B ) was observed in OVEA6 and OVCA 429 cells only ... Together, our findings indicate that multiple proteinases are important in ovarian cell invasion and implicate
EGF induction of
MMP-9 and migration as key components of more aggressive ligand induced invasion
Piedagnel et al., J Biol Chem 1999
:
Matrix metalloproteinase 2 (MMP2) and
MMP9 are produced by kidney collecting duct principal cells but are differentially
regulated by SV40 large-T, arginine vasopressin, and
epidermal growth factor ... Arginine vasopressin and
epidermal growth factor , which exert opposite hydroosmotic effects in the collecting duct, also exhibited contrasted
effects on
MMP9 synthesis
McCawley et al., J Biol Chem 1999
:
We have shown previously that although many receptor tyrosine kinase ligands are mitogens for keratinocytes, cell migration and induction of the 92-kilodalton
gelatinase/matrix metalloproteinase (MMP)-9 are selectively
regulated by the
epidermal growth factor and scatter factor/hepatocyte growth factor receptors