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NPY6R — RAF1

Text-mined interactions from Literome

Lee et al., J Biol Chem 2004 : Unlike PMA/H2O2 treatment, which showed transient activation, PP2 mediated Raf-1 activation was sustained and continued to increase through 4 h of treatment ... Transient transfection studies with a dominant negative mutant of Ras ( N19Ras ) indicated that this PP2 induced activation of Raf-1 was Ras independent ... Interestingly, mutation of the reported Raf-1 Src family tyrosine kinase phosphorylation site by conversion of tyrosines 340 and 341 to phenylalanine ( YY340/341FF Raf ) had limited effect on the ability of PP2 to induce significant stimulation of Raf-1 kinase activity ... This PP2 effect resulted in significant and sustained Ras independent activation of Raf-1 by PMA and H2O2
Lee et al., Biochem Biophys Res Commun 2006 (MAP Kinase Signaling System) : Recently we demonstrated that PP2 ( 4-amino-5- ( 4-chloro-phenyl ) -7- ( t-butyl ) pyrazolo [ 3,4-d ] pyrimidine ), a potent and selective inhibitor of the Src-family tyrosine kinase, markedly enhanced Ras independent activation of Raf-1 by the combination of phorbol myristate acetate ( PMA ) and hydrogen peroxide ( H ( 2 ) O ( 2 ) ) ... PP2 had limited effect on the ability of PMA/H ( 2 ) O ( 2 ) to induce significant phosphorylation of MEK/ERK proteins in both Spry2 knockdown and control cells, indicating that PP2 mediated activation of Raf-1 did not potentiate signaling through the downstream MEK/ERK pathway