◀ Back to TNF

PTGS2 — TNF

Pathways - manually collected, often from reviews:

• OpenBEL Selventa BEL large corpus: PTGS2 → TNF (increases)
  Evidence: In the human lung adenocarcinoma A549 cells, even though these cells possessed significant basal COX-2 levels (52), TNF was found to further induce COX-2 within 2 h, leading to significant PGE2 within a time frame similar to that found in L929 cells (Fig. 7A).
• OpenBEL Selventa BEL large corpus: PTGS2 → TNF (increases) Chen et al., J Immunol 2000*
  Evidence: TNF-a induced COX-2 expression in NCI-H292 cells. When the cells were exposed to 10 ng/ml of IL-1b, TNF-a, or IFN-g, 1 mg/ml of LPS, or 1 mM TPA, only TNF-a and TPA stimulated COX-2 expression (data not shown).
• OpenBEL Selventa BEL large corpus: PTGS2 → TNF (increases) Nakata et al., Cancer Sci 2003*
  Evidence: TNF-alpha significantly induced COX-2 expression in Oka-C-1 and MI-4 cells. The induction seemed to be stronger in Oka-C-1 cells than in MI-4 cells. Cox2 inhibitor, NS-398 suppressed TNF-alpha induced Cox2 expression.

Text-mined interactions from Literome

Yucel-Lindberg et al., J Dent Res 1999 : The study indicates that the synergistic stimulation of prostaglandin production by IL-1beta, and TNFalpha is mediated partly at the level of COX-2 and partly at the level of PLA2 and that PKC is involved in the signal transduction of the synergy between the two cytokines
Chen et al., Life Sci 1999 : However, the activation of NF-kappaB was not related to the expression of COX-2 induced by TNF-alpha since calpain inhibitor I blocked the activation of NF-kappaB but not the expression of COX-2 mRNA or protein
Alaaeddine et al., Arthritis Rheum 1999 (Osteoarthritis) : IL-4, IL-10, and IL-13 reduced the TNFalpha induced COX-2 level, and IL-4 and IL-10 reduced the cPLA2 level
Díaz-Cazorla et al., J Am Soc Nephrol 1999 : Oligonucleotide decoy experiments confirmed the importance of nuclear factor kappaB (NF-kappaB) activation for COX-2 induction by IL-1beta/TNF-alpha
Tsao et al., Prostaglandins Other Lipid Mediat 1999 (Encephalitis...) : TNFalpha induced cyclooxygenase 2 not only increases the vasopermeability of blood-brain barrier but also enhances the neutrophil survival in Escherichia coli induced brain inflammation ... Cyclooxygenase-2 and TNFalpha were co-localized on the arterioles as well as the infiltrating neutrophils by serial-section staining, indicating that cyclooxygenase-2 was induced by TNFalpha ... This suggests that TNFalpha stimulated cyclooxygenase-2 induction play an important role on E. coli induced brain inflammation
Ferreri et al., Am J Physiol 1999 : These data suggest that TNF mediated induction of COX-2 protein expression accounted for the lag-time required for this cytokine to inhibit 86Rb uptake in MTAL cells
Hernández et al., J Neurochem 1999 (Astrocytoma...) : In keeping with these findings, TNF-alpha produced an increase of COX-2 expression, as judged from both RT-PCR studies and immunoblot of COX-2 protein, and a long lasting activation of NF-kappaB
Minghetti et al., J Neuropathol Exp Neurol 1999 (Encephalitis) : Microinjection of IFN-gamma did not alter COX-2-ir, whereas TNF-alpha or IL-1beta injection induced a moderate COX-2-ir in the perivascular cells of a few blood vessels close to the injection site, and in very few of the infiltrating neutrophils
Yamamoto et al., FEBS Lett 2000 (Bone Resorption) : In murine osteoblastic MC3T3-E1 cells which produced prostaglandin E2 as a bone resorption factor, the cyclooxygenase-2 induction by tumor necrosis factor alpha (TNFalpha) was suppressed by dexamethasone with an IC ( 50 ) of 1 nM ... We showed that the compound suppressed the TNFalpha dependent cyclooxygenase-2 induction with an IC ( 50 ) of as low as about 30 nM as demonstrated experimentally by catalytic activity assay, Northern blot analysis and promoter analysis
Potter et al., Prostaglandins Leukot Essent Fatty Acids 1999 : A rapid increase in PGHS-2 ( but not PGHS-1 ) mRNA expression was observed in response to TNF-alpha and IL-1beta
Blumenstein et al., Placenta 2000 : TNF-alpha increased PG output and PGHS-2 expression independent of cell type
Young et al., Circ Res 2000 : Cyclooxygenase-2 is required for tumor necrosis factor-alpha- and angiotensin II-mediated proliferation of vascular smooth muscle cells ... Tumor necrosis factor-alpha (TNF-alpha) and angiotensin II (Ang II) induced a transient increase in vascular smooth muscle cell ( VSMC ) cyclooxygenase-2 (COX-2) mRNA accumulation, without affecting COX-1 mRNA levels
Huang et al., Biochem Pharmacol 2000 : Differential regulation of cyclooxygenase-2 (COX-2) mRNA stability by interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) in human in vitro differentiated macrophages ... In this study, we examined the induction of COX-2 expression by interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) in human primary in vitro differentiated macrophages
Paik et al., J Biol Chem 2000 (Adenocarcinoma...) : An activator of peroxisome proliferator activated receptor gamma, 15-deoxy-Delta ( 12,14 ) -prostaglandin J ( 2 ), also induces COX-2 expression and inhibits TNFalpha induced NFkappaB activation and COX-2 expression
Chen et al., J Immunol 2000 : TNF-alpha induced cyclooxygenase-2 expression in human lung epithelial cells : involvement of the phospholipase C-gamma 2, protein kinase C-alpha, tyrosine kinase, NF-kappa B-inducing kinase, and I-kappa B kinase 1/2 pathway ... Tyrosine kinase inhibitors ( genistein or herbimycin ) or phosphoinositide-specific phospholipase C inhibitor ( U73122 ) blocked TNF-alpha induced COX-2 expression ... NF-kappaB DNA-protein binding and COX-2 promoter activity were enhanced by TNF-alpha , and these effects were inhibited by genistein, U73122, staurosporine, or pyrolidine dithiocarbamate
Kniss et al., Placenta 2001 : Blockade NF-kappaB activation prohibits TNF-alpha induced cyclooxygenase-2 gene expression in ED27 trophoblast-like cells
Chen et al., Mol Pharmacol 2001 : Tumor necrosis factor-alpha induced cyclooxygenase-2 expression via sequential activation of ceramide dependent mitogen activated protein kinases, and IkappaB kinase 1/2 in human alveolar epithelial cells ... The role of p44/42 mitogen activated protein kinase ( MAPK ), p38, and c-Jun NH ( 2 ) -terminal kinase ( JNK ) in tumor necrosis factor (TNF)-alpha induced cyclooxygenase (COX)-2 expression was studied in NCI-H292 epithelial cells ... TNF-alpha mediated COX-2 expression and COX-2 promoter activity were inhibited by the MAPK kinase inhibitor PD98059 or the p38 inhibitor SB203580 ... Glutathione, a neutral SMase inhibitor, attenuated TNF-alpha- or SMase induced activation of MAPKs, COX-2 expression, and COX-2 promoter activity
Wang et al., J Physiol Pharmacol 2000 (Hypercalcemia) : As TNF production is calcium-sensitive in some cells types, we postulate that these effects involve the regulation of COX-2 expression via a TNF dependent mechanism
Efsen et al., Hepatology 2001 : Quiescent HSC did not show detectable expression of COX-2, which became evident after activation in culture, and while TNF-alpha and IL-1alpha markedly increased the expression of COX-2 , IFN-gamma did not have any effects
Delerive et al., EMBO Rep 2001 (Inflammation) : We demonstrated that ROR alpha is expressed in human primary smooth-muscle cells and that ectopic expression of ROR alpha1 inhibits TNFalpha induced IL-6, IL-8 and COX-2 expression in these cells
Weaver et al., Gastroenterology 2001 : Regulatory role of phosphatidylinositol 3-kinase on TNF-alpha induced cyclooxygenase 2 expression in colonic epithelial cells ... In HT-29 and Caco-2 colonic epithelial cells, COX-2 expression was induced by either TNF-alpha or interleukin (IL)-1alpha as observed by Northern and Western analyses
Subbarayan et al., Cancer Res 2001 (Prostatic Neoplasms) : Differential expression of cyclooxygenase-2 and its regulation by tumor necrosis factor-alpha in normal and malignant prostate cells ... Because COX-2 levels were low in prostate smooth muscle cells, prostate stromal cells, and tumor cells, we also examined whether COX-1 and COX-2 gene expression was elevated in response to tumor necrosis factor-alpha (TNF-alpha) , a strong inducer of COX-2 expression ... TNF-alpha induction of COX-2 and prostaglandin E2 correlated inversely with induction of apoptosis ... Although it is low in stromal and tumor cells, COX-2 expression is induced by TNF-alpha in these cells, and this responsiveness may play an important role in prostate cancer progression
Cinatl et al., J Immunol 2001 : Cyclooxygenase-2 expression and PGE2 release was increased by TPA and TNF-alpha but not by HCMV infection
Moore et al., J Appl Physiol 2001 : TNF-alpha alone, at concentrations up to 10 ng/ml, had no effect on COX-2 protein expression ; at concentrations as low as 0.1 ng/ml, it significantly enhanced the ability of IL-1 beta ( 0.2 ng/ml ) to induce COX-2 and to increase PGE ( 2 ) release ... IL-1 beta and TNF-alpha in combination also significantly enhanced COX-2 promoter activity, indicating that synergism between the cytokines is mediated at the level of gene transcription
Yoshimatsu et al., Clin Cancer Res 2001 (Carcinoma, Non-Small-Cell Lung...) : Tumor necrosis factor-alpha induced mPGES and COX-2 in NSCLC cell lines but had no effect on the expression of either enzyme in a nontumorigenic bronchial epithelial cell line
Blais et al., J Neuropathol Exp Neurol 2001 : Inhibitory action of nitric oxide on circulating tumor necrosis factor induced NF-kappaB activity and COX-2 transcription in the endothelium of the brain capillaries ... This study investigated the hypothesis that nitric oxide ( NO ) acts as an endogenous modulator of TNF induced NF-kappaB signaling and COX-2 transcription in the endothelium of the cerebral capillaries ... These results indicate that eNOS derived NO acts as an endogenous inhibitor of TNF-alpha induced NF-kappaB activity and COX-2 transcription in the endothelium of the cerebral capillaries
Yoshimatsu et al., Clin Cancer Res 2001 (Adenocarcinoma...) : Tumor necrosis factor-alpha induced both mPGES and COX-2 , but the time course and magnitude of induction differed
Yamada et al., Clin Exp Immunol 2002 (Urinary Bladder Neoplasms) : NS398, a specific COX-2 inhibitor, and indomethacin ( IM ), a COX-1 and COX-2 inhibitor, enhanced viable BCG induced cytotoxic activity and IFN-gamma and TNF-alpha production of PEC
Lin et al., J Endod 2002 (Pulpitis) : In contrast, PGE2 or indomethacin failed to modify the stimulatory effect of IL-1alpha or TNF-alpha on COX-2 gene expression
Nishikawa et al., J Periodontal Res 2002 : TNFalpha also increased COX-2 mRNA levels
Basu et al., Glia 2002 (Brain Injuries...) : These studies demonstrated that IL-1beta, TNFalpha , IL-6, but not IFN-gamma increase the expression of Cox-2 , whereas they all increase the expression of HCK and FAK ... However, TGFbeta1 was ineffective in antagonizing the induction of Cox-2 by either IL-6 or TNFalpha
Banerjee et al., Mol Cell Biochem 2002 : Inhibition of TNFalpha induced cyclooxygenase-2 expression by amentoflavone through suppression of NF-kappaB activation in A549 cells
Nakao et al., Mol Cell Biochem 2002 : In this paper, we investigated the involvement of NFkappaB on TNF-alpha mediated prostaglandin E2 ( PGE2 ) release and COX-2 gene expression in human gingival fibroblasts (HGF) ... TNF-alpha induced PGE2 release and COX-2 mRNA accumulation in a time- and concentration dependent manner in HGF
Chanani et al., Circulation 2002 (Inflammation) : Both LPS and TNF-alpha induced significant NFkappaB activation, cyclooxygenase-2 (COX-2) expression, and inducible NO synthase (iNOS) and cytokine production ; with the exception of COX-2 expression, all were significantly reduced by amrinone, beginning at concentrations of 10 to 50 micro mol/L
Ashikawa et al., J Immunol 2002 : Piceatannol abrogated the expression of TNF induced NF-kappaB dependent reporter gene and of matrix metalloprotease-9, cyclooxygenase-2 , and cyclin D1
Kaltschmidt et al., BMC molecular biology 2002 : In the murine neuroblastoma cell line NB-4, which is characterized by constitutive NF-kappaB activity, COX-2 promoter activity could not be further increased with PMA or TNF
Relić et al., Lab Invest 2002 : In primary chondrocytes, TNF-alpha induced expression of the antiapoptotic protein Cox-2 , which persisted in the presence of SNP, and a specific Cox-2 inhibitor significantly blocked the TNF-alpha protective effect
Yan et al., J Lipid Res 2003 : Tumor necrosis factor-alpha (TNFalpha) significantly up-regulated COX-2 expression ( approximately 2-fold ) in differentiating 3T3-L1 cells, whereas similar effect was not observed with COX-1 expression ... Abrogating the induced COX-2 activity reversed the TNFalpha induced inhibition of differentiation by approximately 70 %, implying a role for COX-2 in mediating TNFalpha signaling
Yao et al., Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 2003 (Arthritis, Rheumatoid) : Results showed that neither FK506 nor TP at lower concentration ( 10 microg/L ) alone affected TNF alpha induced COX-2 , iNOS expression and production of PGE2, NO in synovial cells
Chang et al., J Endod 2003 (Pulpitis) : The aim of the present study was to investigate the effects of interleukin (IL)-1alpha and tumor necrosis factor-alpha (TNF-alpha) on the expression of COX-2 mRNA gene and protein in cultured human pulp cells ... In addition, both IL-1alpha and TNF-alpha up-regulated COX-2 protein expression by human pulp cells
Nakata et al., Cancer Sci 2003 (Disease Progression...) : Inflammatory cytokines, tumor necrosis factor-alpha (TNF-alpha) and interleukin (IL)-1beta stimulated the expression of G-CSF, GM-CSF, and cyclooxygenase (COX)-2 in the two cell lines
Gottstein et al., Br J Nutr 2003 (Arteriosclerosis) : In a macrophage cell line ( RAW 264.7 ) activated with interferon gamma plus lipopolysaccharide, both isoflavones were found to inhibit NO production and tumour necrosis factor alpha (TNF-alpha) secretion dose-dependently, but they did not affect mRNA levels for inducible nitric oxide synthase and cyclo-oxygenase-2
Schmeck et al., Inflammation 2003 : Therefore, RhoA/Rac1/Cdc42 contribute to NF-kappaB dependent LPS- and TNFalpha induced expression of PGHS-2 in EC but had no effect on the activity of expressed COX-1 and COX-2
Jiang et al., Mol Cell Biochem 2003 : In this study, the effect of phorbol 12-myristate 13-acetate ( PMA ), tumor necrosis factor alpha (TNFalpha), lipopolysaccharide (LPS) and macrophage-colony stimulating factor ( M-CSF ) on AA release and prostaglandin E2 ( PGE2 ) production and the expression of cPLA2 and COX-1 and -2 were investigated in U937 human pre-monocytic cells and fully differentiated macrophages
D'Hooghe et al., Curr Opin Obstet Gynecol 2003 (Disease Models, Animal...) : Recent research shows a very promising role for new hormonal medication ( aromatase inhibitors, estrogen and progesterone receptor modulators ) and anti-inflammatory drugs ( tumor necrosis factor-alpha inhibitors, matrix metalloproteinase inhibitors, cyclooxygenase-2 inhibitors ) in the management of endometriosis
Zhang et al., J Am Dent Assoc 2003 (Chronic Disease...) : The results showed that lipopolysaccharide and tumor necrosis factor alpha, or TNF-alpha , induced COX-2 in macrophages, while IL-1beta and TNF-alpha induced COX-2 in oral epithelial cells
Kwon et al., Arch Pharm Res 2003 : Salicylate inhibited the IL-1beta and TNF-alpha induced COX-2 expressions, regulated the activation of ERK, IKK and IkappaB degradation, and the subsequent activation of NF-kappaB, in neonatal rat ventricular cardiomyocytes ... The inhibition of the ERK pathway, with a selective inhibitor, PD098059, blocked the expressions of IL-1beta and TNF-alpha induced COX-2 and PGE2 release
Okuda et al., Biol Reprod 2004 : When cultured bovine stromal cells were exposed to TNFalpha ( 0.006-0.6 nM ) for 24 h, the production of PGF2alpha and cyclooxygenase (COX)-2 gene expression were stimulated by TNFalpha ( 0.06-0.6 nM, P < 0.05 ) ... Moreover, the stimulatory effect of TNFalpha ( 0.6 nM ) on COX-2 gene expression was completely blocked by IFNtau ( 30 ng/ml ; P < 0.05 ), although the gene expression of COX-2 was not influenced by IFNtau ... The overall results indicate that the stimulatory effect of TNFalpha on PGF2alpha production is mediated by the up-regulation of COX-2 gene expression and suggest that one of the mechanisms of the inhibitory effect of IFNtau on luteolysis is the inhibition of TNFalpha action in PGF2alpha production in the stromal cells by the down-regulation of COX-2 gene expression stimulated by TNFalpha
Sakurai et al., J Cell Physiol 2004 : The catalytic Sec residue of TrxR1, which is essential for reducing Trx, was required for this NF-kappa B activation, and aurothiomalate, an inhibitor of TrxR, suppressed TNF alpha induced activation of NF-kappa B and the expression of NF-kappa B-targeted proinflammatory genes such as E-selectin and cyclooxygenase-2
Ferreri et al., Thromb Res 2003 : TNFalpha regulates renal COX-2 in the rat thick ascending limb ( TAL )
Konturek et al., Dig Dis Sci 2003 (Helicobacter Infections...) : ( 4 ) COX-2, PPARgamma, Bcl-2, and survivin were overexpressed in gastric tumor, but the inhibition of COX-2 activity by Vioxx resulted in a significant reduction in serum and tumor levels of progastrin and gastrin and serum IL-8 and TNF-alpha levels, suggesting that gastrin and proinflammatory cytokines could mediate the up-regulation of COX-2 in gastric cancerogenesis
Takada et al., J Biol Chem 2004 : Flavopiridol also inhibited the expression of the TNF induced NF-kappaB regulated gene products cyclin D1, cyclooxygenase-2 , and matrix metalloproteinase-9
Premyslova et al., J Clin Endocrinol Metab 2003 (Bacterial Infections...) : However, TNF-alpha increased both mPGES and COX-2 protein expression in these cells ... In CT cells in culture, IL-1beta and TNF-alpha increased both mPGES and COX-2 protein levels ... We have evaluated the effect of chorioamnionitis on the protein expression of microsomal and cytosolic prostaglandin E ( 2 ) synthases ( mPGES and cPGES ) in preterm human placentae ( PL ) and fetal membranes ( FM ), by Western blot and immunohistochemistry, as well as the regulatory effect of IL-1beta and TNF-alpha on mPGES, cPGES, and cyclooxygenase (COX)-2 expression in villous trophoblast ( VT ) and chorion trophoblast ( CT ) cell cultures
Lin et al., Cell Signal 2004 : Tumor necrosis factor-alpha induced cyclooxygenase-2 expression in human tracheal smooth muscle cells : involvement of p42/p44 and p38 mitogen activated protein kinases and nuclear factor-kappaB ... TNF-alpha markedly increased COX-2 expression and PGE2 synthesis in a time- and concentration dependent manner, whereas COX-1 remained unaltered ... Furthermore, TNF-alpha induced activation of nuclear factor-kappaB (NF-kappaB) reversely correlated with the degradation of IkappaB-alpha in HTSMCs. TNF-alpha induced COX-2 expression and PGE2 synthesis was also inhibited by NF-kappaB inhibitor pyrrolidinedithiocarbamate ( PDTC ) ... Tyrosine kinase inhibitor ( genistein ), phosphatidylcholine-specific phospholipase C ( PC-PLC ) inhibitor ( D-609 ) and PKC inhibitor ( GF109203X ) attenuated TNF-alpha induced COX-2 expression and PGE2 synthesis in HTSMCs. TNF-alpha induced COX-2 expression and PGE2 synthesis were also inhibited by PD98059 ( an inhibitor of MEK1/2 ) and SB203580 and SB202190 ( inhibitors of p38 MAPK ), respectively, suggesting the involvement of p42/p44 and p38 MAPKs in these responses
Schmitz et al., Domest Anim Endocrinol 2004 (Mastitis, Bovine) : In LPS treated quarters, tumor necrosis factor-alpha and cyclooxygenase-2-mRNA expression increased ( P < 0.05 ) to highest values at 3h after LPS challenge
Nanji et al., J Pharmacol Exp Ther 2004 (Fatty Liver...) : Also, nuclear factor kappaB activation and up-regulation of tumor necrosis factor-alpha and cyclooxygenase-2 was also abolished in clofibrate treated rats
Tong et al., Biochem Biophys Res Commun 2004 (Colonic Neoplasms) : Studying the HT-29 colon cancer cell line as a model, we found that Cox-2 expression and activity is increased approximately 25-fold by TNF-alpha
Chae et al., Immunopharmacol Immunotoxicol 2004 : Salicylate treatment inhibited COX-2 expression induced by TNF-alpha/IFN-gamma and regulated the activation of ERK, IKK and IkappaB degradation and subsequent NF-kappaB activation in MC3T3E1 osteoblasts
Min et al., J Rheumatol 2004 (Arthritis, Rheumatoid) : Both IL-1beta and TNF-a upregulated COX-2 mRNA comparably to IL-15, but neither IL-2 nor interferon-g had any effect on the COX-2 mRNA level
Kitatani et al., Cell Signal 2004 : Pretreatment with exogenous sphingomyelinase ( SMase ) dose-dependently enhanced TNF-alpha stimulated increases in COX-2 protein and sPLA ) activity
Huang et al., Carcinogenesis 2004 (Neoplasm Invasiveness) : In Matrigel assays, ICAM-1 and COX-2 expressions induced by TNF-alpha elicited A549 and NCI-H292 cell invasion, respectively, and these effects were inhibited by both compounds
Shishodia et al., J Immunol 2004 (Carcinoma, Non-Small-Cell Lung...) : The COX-2 promoter, which is regulated by NF-kappa B, was also inhibited by celecoxib, and this inhibition correlated with suppression of TNF induced COX-2 expression
Gray et al., Mol Pharmacol 2004 : We also observed that tumor necrosis factor (TNF)-alpha , platelet activating factor (PAF), and lipopolysaccharide (LPS) induced COX-2 and increased MUC5AC production that was blocked by celecoxib, suggesting a common signaling pathway of inflammatory mediator induced MUC5AC production in NHTBE cells
Park et al., Int J Biochem Cell Biol 2004 (Bone Resorption) : In contrast, the presence of TGF-beta reduced COX-2 mRNA level, PGE ( 2 ) biosynthesis and bone resorption induced by IL-1beta, TNF-alpha , IL-6 or a combination thereof
Nakayama et al., Cell Biol Int 2004 (Osteosarcoma) : We used RT-PCR to examine the effects of TNF-alpha on bone sialoprotein ( BSP ), core binding factor a1 (Cbfa1), osterix, alpha 1 ( I ) collagen, cyclooxygenase-2 (COX-2) , interleukin-6 (IL-6), cathepsin B, cathepsin L and tissue inhibitors of metalloproteinase-1 ( TIMP-1 ) ... TNF-alpha ( 10ng/ml ) increased BSP, IL-6 and COX-2 mRNA levels after 3h, reaching maximal levels at 12 h. Cbfa1 mRNA levels increased after 3 h, but decreased by 24 h. Osterix, cathepsin B, cathepsin L and TIMP-1 mRNA levels did not change after stimulation with TNF-alpha
Fehrenbacher et al., Pain 2005 : The addition of IL-1 receptor antagonist blocked the induction of COX-2 expression by IL-1beta, but did not alter TNFalpha stimulated increases in COX-2 , indicating that the mechanism of TNFalpha is not limited to increasing the expression of IL-1beta ... The basal and TNFalpha induced expression of COX-2 was not dependent on the presence of NGF in the growth media ... These results indicate that TNFalpha and IL-1beta induce the functional expression of COX-2 but not EP receptors in DRG cells in culture and suggest that cytokine induced sensitization of sensory neurons is secondary to prostaglandin production and not alterations in EP receptors
Mustafa et al., J Clin Periodontol 2005 : The cytokine TNFalpha enhanced the expression of mRNA as well as the protein levels of both COX-2 and mPGES-1 and subsequently the production of PGE2 in gingival fibroblasts
Cavin et al., Biochem Biophys Res Commun 2005 : The inhibition of TNF-alpha dependent induction of COX-2 expression was mediated by an inhibition of NF-kappaB activation
Eligini et al., Cardiovasc Res 2005 : Diversity and similarity in signaling events leading to rapid Cox-2 induction by tumor necrosis factor-alpha and phorbol ester in human endothelial cells ... Conversely, MAPK p38 is critical for Cox-2 induction by TNF alpha
Tong et al., J Biol Chem 2005 (Colonic Neoplasms) : A nuclear run-on assay showed that TNF-alpha increased Cox-2 transcription, whereas butyrate was suppressive
Vila-del Sol et al., J Immunol 2005 : Involvement of TNF and NF-kappa B in the transcriptional control of cyclooxygenase-2 expression by IFN-gamma in macrophages
Brueckmann et al., Thromb Haemost 2005 : RhAPC further increased the stimulating effect of tumor necrosis factor-alpha (TNF-alpha) and thrombin on COX-2-mRNA-levels
Park et al., Biochemistry 2005 : Inhibition of NF-kappaB by cobrotoxin resulted in reductions in the LPS induced expressions of COX-2, iNOS, cPLA(2), IL-4, and TNF-alpha in astrocytes and in COX-2 expression induced by SNP, LPS, and TNF-alpha in astrocytes
Yang et al., Oncogene 2005 (Ovarian Neoplasms) : Loss of TNF-alpha regulated COX-2 expression in ovarian cancer cells ... Tumor necrosis factor alpha (TNF-alpha) is a potent inducer of COX-2 expression in the ovarian surface epithelium and this regulation is a critical step in ovulation ... We observed that TNF-alpha stimulated COX-2 expression in human primary and immortalized epithelial ( HIO ) cell lines ... In NIH : OVCAR-5, the only ovarian cancer cell line expressing COX-2, signal pathway inhibitors no longer affected TNF-alpha induced COX-2 expression
Aggarwal et al., Mol Pharmacol 2006 (Neoplasm Metastasis) : Such TNF induced NF-kappaB regulated gene products involved in cellular proliferation [ cyclooxygenase-2 (COX-2), cyclin D1, and c-myc ], antiapoptosis [ inhibitor of apoptosis protein (IAP)1, IAP2, X-chromosome linked IAP, Bcl-2, Bcl-x ( L ), Bfl-1/A1, TNF receptor associated factor 1, and cellular Fas associated death domain protein-like interleukin-1beta converting enzyme inhibitory protein-like inhibitory protein ], and metastasis ( vascular endothelial growth factor, matrix metalloproteinase-9, and intercellular adhesion molecule-1 ) were also down-regulated by curcumin ... COX-2 promoter activity induced by TNF was abrogated by curcumin
Shun et al., Ann Otol Rhinol Laryngol 2005 (Nasal Polyps) : The Northern blot analysis showed that TNF-alpha stimulated the expression of CCL2 and COX-2 genes, and the synthesis of CCL2 messenger RNA was COX-2 dependent
Lianxu et al., Osteoarthritis Cartilage 2006 (Osteoarthritis, Knee) : The NF-kappaBp65-specific siRNA inhibited the expression of NF-kappaBp65 and activation of NF-kappaB, reducing significantly the expression of COX-2 , NOS-2 and MMP-9 induced by interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) in cultured chondrocytes
Adams et al., J Agric Food Chem 2006 (Inflammation) : At a concentration of 50 mg/L PJ significantly suppressed TNFalpha induced COX-2 protein expression by 79 % ( SE = 0.042 ), total pomegranate tannin extract ( TPT ) 55 % ( SE = 0.049 ), and punicalagin 48 % ( SE = 0.022 )
Kim et al., FEBS Lett 2006 (Disease Models, Animal...) : Western blot analysis and luciferase activity assay showed that anthocyanins inhibited TNF-alpha induced vascular cell adhesion molecule-1, intracellular adhesion molecule-1, and cyclooxygenase-2 levels, which is through NF-kappaB dependent pathway
Jin et al., J Ethnopharmacol 2006 (Bone Resorption...) : TGF-beta, YJ ( 100microg/ml ) and their combinations of YJ+TGF-beta reduced the COX-2 mRNA level, PGE2 biosynthesis and bone resorption induced by IL-1beta, TNF-alpha , IL-6 or their combination
Yu et al., Hepatology 2006 (Choline Deficiency...) : In conclusion, induction of COX-2 occurs in association with NF-kappaB activation and upregulation of TNF-alpha , IL-6, and ICAM-1 in MCD diet induced steatohepatitis
Tong et al., Carcinogenesis 2006 (Adenocarcinoma...) : Interleukin (IL) 1beta, tumor necrosis factor-alpha (TNF-alpha) or phorbol ester [ phorbol 12-myristate 13-acetate ( PMA ) ] induced the expression of COX-2 , as revealed by western blot analysis
Yan et al., J Cell Sci 2006 (Cell Transformation, Neoplastic) : The induction of COX-2 by TNF-alpha was abolished by knockdown of NFAT3 with its siRNA, while the induction of iNOS was not effected
Brenneis et al., FASEB J 2006 : TNFalpha induced COX-2 and mPGES-1 expression in neurons, followed by formation of PGE2, which was blocked by a selective COX-2 inhibitor ... SC-560 treatment neither altered TNFalpha induced COX-2 or mPGES-1 expression nor did the addition of the calcium ionophore A23187 or arachidonic acid reverse the inhibition by SC-560
Nishimura et al., Biosci Biotechnol Biochem 2006 : TNF-alpha preferentially promoted gene expression of cyclooxygenase (COX)-2 without affecting that of COX-1
Ke et al., J Dent Res 2007 (Temporomandibular Joint Disorders) : Role of NF-kappaB in TNF-alpha induced COX-2 expression in synovial fibroblasts from human TMJ ... By reverse-transcriptase/polymerase chain-reaction ( RT-PCR ) and Western blotting analysis, TNF-alpha induced a dose- and time dependent increase in COX-2 expression ... Pre-treatment with pyrolidine dithiocarbamate ( PDTC ), one of the NF-kappaB inhibitors, prevented binding to the COX-2 promoter and expression of COX-2 protein in response to TNF-alpha ... These findings indicate that activation of NF-kappaB is responsible for TNF-alpha induced COX-2 expression in synovial fibroblasts from the TMJ
Wu et al., Prostaglandins Other Lipid Mediat 2007 : In this review, we provide evidence for reduced COX-2 transcriptional expression in response to phorbol esters ( PMA ), lipopolysaccharide (LPS), interleukin-1beta (IL-1beta) and tumor necrosis factor alpha (TNFalpha)
Karrasch et al., PloS one 2007 (Colitis) : In vitro, luteolin sensitized colonic epithelial HT29 cells to TNFalpha induced apoptosis, caspase 3 activation, DNA fragmentation and reduced TNFalpha induced C-IAP1, C-IAP2 and COX-2 gene expression
Candelario-Jalil et al., J Pharmacol Exp Ther 2007 : TNF-alpha induced a significant up-regulation of gelatinase B ( MMP-9 ), stromelysin-1 ( MMP-3 ), and COX-2
L'Eplattenier et al., J Vet Intern Med 2007 (Dog Diseases...) : In addition, a neoplastic and a nonneoplastic canine prostatic cell line were used to investigate the effects of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha) , phorbol 12-myristate 13-acetate ( PMA ), epithelial growth factor (EGF), and specific signal transduction pathway inhibitors on COX-2 expression ... In vitro, TNF-alpha , IL-6, and EGF increased COX-2 expression in nonneoplastic cells but not in PCA cells, where baseline expression was high
Wu et al., Int Immunopharmacol 2007 (Inflammation) : After different kinds of cyclooxygenase (COX) inhibitors were administrated, it showed that the effects of DHAP on TNF-alpha and apoptosis were COX-2 dependent
Konturek et al., Dig Dis Sci 2008 (Adenocarcinoma...) : Ghrelin caused a decrease in TNFalpha induced COX-2 and IL-1beta expression in OE-19 cells
Ahn et al., Cancer Res 2007 (Skin Neoplasms) : The induction of various antiapoptotic gene products ( MMP-9, cyclin D1, COX-2 , IAP1, IAP2, Bcl-2, cFLIP, and XIAP ) by TNF was also abolished in NQO2-/- cells
Chida et al., Biochem Biophys Res Commun 2007 (Fever) : Only IL-1alpha, but not TNFalpha , induced IL-6 in the IL-1alpha/beta/TNFalpha KO mouse brain, while both cytokines induced cyclooxygenase (Cox)-2
Sareila et al., Int Immunopharmacol 2008 : The JAK3 inhibitor had no effect on COX-2 expression, and TNF-alpha production was slightly inhibited only at higher drug concentrations ( 30 microM )
Schell et al., Endocrinology 2008 : TNF-alpha furthermore strongly enhanced expression and/or synthesis of other inflammatory molecules, namely IL-6 and cyclooxygenase-2
Lee et al., J Immunol 2008 : Specifically in response to TNF-alpha , TGF-beta, and LPS, protein expression of cyclooxygenase (COX)-2 and downstream PGE synthase was up-regulated with coordinate kinetics, whereas COX-1 and PGIS were constitutively expressed
Bianchi et al., Neurobiol Aging 2010 (Encephalitis...) : We show here that : ( 1 ) S100B also stimulates AP-1 transcriptional activity in microglia via RAGE dependent activation of JNK ; ( 2 ) S100B upregulates IL-1beta and TNF-alpha expression in microglia via RAGE engagement ; and ( 3 ) S100B/RAGE induced upregulation of COX-2 , IL-1beta and TNF-alpha expression requires the concurrent activation of NF-kappaB and AP-1
Wang et al., Metabolism 2008 : The p38 MAPK inhibitor SB203580 blocked TNF-alpha mediated induction of COX-2 protein expression, suggesting a regulatory mechanism through p38 MAPK signaling ... Furthermore, silencing of the caveolin-1 gene completely blocked TNF-alpha induced production of COX-2 and PGE ( 2 ) and significantly reduced the amplified response of linoleic acid plus TNF-alpha
Snider et al., FASEB J 2009 (Body Weight...) : Unlike WT mice, SK1 ( -/- ) mice failed to show colonic COX-2 induction despite an exaggerated TNF-alpha response ; thus implicating for the first time SK1 in TNF-alpha mediated COX-2 induction in vivo
Matsuzuka et al., Mol Cell Biochem 2009 (Lung Neoplasms) : In addition, Ang II synergistically stimulated the induction of COX-2 by pro-inflammatory cytokines, IL-1beta, or TNF-alpha
Jiang et al., Zhongguo Zhong Xi Yi Jie He Za Zhi 2008 (Disease Models, Animal...) : PrG ( 40 mg x kg ( -1 ) d ( -1 ) ) could decrease the serum level of inflammatory factor TNF-alpha , and slightly inhibit COX-2 and ICAM-1 protein expression in ischemic myocardium of AMI rats
Bansal et al., Scand J Immunol 2009 : Inhibition of TNF-alpha induced cyclooxygenase-2 expression by Mycobacterium bovis BCG in human alveolar epithelial A549 cells ... Our results demonstrates that Mycobacterium bovis BCG ( M. bovis BCG ) downregulates tumour necrosis factor-alpha (TNF-alpha) induced COX-2 gene expression in alveolar epithelial cells by inhibiting the phosphorylations of Raf-1 and p38 kinases
Martinet et al., Eur J Immunol 2009 : COX-2 expression and PGE ( 2 ) production by MSC were not constitutive, but were induced by IFN-gamma and TNF-alpha secreted by activated Vgamma9Vdelta2 T cells
Itatsu et al., Am J Pathol 2009 (Bile Duct Neoplasms...) : The TNF-alpha/TNF-receptor 1 (TNF-R1) interaction induced MMP-9 production and activation, as well as COX-2 overexpression and PGE2 production, and increased the migration of CC cells ... In conclusion, we propose a novel signaling pathway of MMP-9 up-regulation in CC cells such that TNF-alpha induces the activation of COX-2 and PGE2 via TNF-R1 followed by the up-regulation of MMP-9 via the PGE2 ( EP2/4 ) receptor
Barakat et al., Naunyn Schmiedebergs Arch Pharmacol 2009 (Brain Ischemia...) : Furthermore, OGD and the NF-kappaB activator tumor necrosis factor stimulated the expression of cPLA-2, cyclooxygenase-2 (COX-2), and mPGES-1 and increased the release of PGE ( 2 ) from neurons
Fu et al., J Vet Med Sci 2009 (Inflammation) : Furthermore, vaspin did not decrease the TNF-alpha ( 24 hr ) induction of vascular cell adhesion molecule-1, intercellular adhesion molecule-1, endothelial selectin, and cyclooxygenase-2 protein expression as well as monocyte chemotactic protein-1, tissue factor, and plasmogen activator inhibitor-1 mRNA expression
Kunze et al., Prostaglandins Other Lipid Mediat 2010 (Inflammation) : IL1beta, TNF-alpha and LPS enhanced the expression of COX-2 and mPGES1 whereas phorbol ester enhanced COX-2 expression only
Kim et al., J Endod 2010 (Bone Resorption) : Stimulation with TNF-alpha and IL-1alpha synergistically increased levels of COX-2 as well as RANKL mRNA and protein expression
Båge et al., BMC genomics 2010 : Inhibitors of JNK and NF-kappaB also decreased the TNFalpha stimulated up-regulation of mPGES-1 and COX-2 as well as PGE2 production
Shah et al., Nat Prod Res 2010 : PBMCs were treated with various concentrations of curcumin, resveratrol and parthenolide and tested for the abilities of these natural products to protect against the LPS induced expression, secretion of the pro-inflammatory cytokines TNFalpha , IL-1beta and IL-6 and activation of the pro-inflammatory COX-2
Frey et al., Lab Invest 2010 (Colonic Neoplasms) : In cultured mouse colon epithelial cells, ErbB4 overexpression resulted in increased levels of cyclooxygenase-2 (COX-2) mRNA and protein ; in contrast, ErbB4 knockdown with siRNA blocked COX-2 accumulation in response to tumor necrosis factor
Waclawik et al., Reproduction 2010 : TNF stimulated PTGS2 and mPGES-1 mRNA, as well as mPGES-1 protein expression and PGE ( 2 ) release on days 11-12 of pregnancy and the estrous cycle
Rajanbabu et al., J Biol Chem 2010 : Studies with the COX-2-specific inhibitor, melaxicam, and with COX-2 overexpressing cells demonstrated the positive regulation of TNF-a and negative regulation of cAMP degradation-specific phosphodiesterase (PDE) 4D by COX-2
Kim et al., J Reprod Med 2009 (Endometriosis) : Tumor necrosis factor-alpha induced cyclooxygenase-2 overexpression in eutopic endometrium of women with endometriosis by stromal cell culture through nuclear factor-kappaB activation ... To investigate the role of NF-kappaB during the induction of COX-2 by TNF-alpha in the eutopic endometrium of women with and without endometriosis using stromal cell culture
Brenneis et al., J Biol Chem 2011 (Disease Models, Animal...) : Interestingly, we found that activation of E-prostanoid ( EP ) 2 and EP4 receptors, but not EP1, EP3, PGI(2) receptor ( IP ), thromboxane A(2) receptor ( TP ), PGD(2) receptor ( DP ), and PGF ( 2 ) receptor ( FP ), efficiently blocked LPS induced tumor necrosis factor a (TNFa) synthesis and COX-2 and mPGES-1 induction as well as prostaglandin synthesis in spinal cultures
Trefiletti et al., Br J Nutr 2011 (Inflammation) : The results showed that L137 significantly inhibited both prostanoid and TNF-a production in lipopolysaccharide primed human monocytes in a dose dependent manner, by inhibiting the COX activity of COX-2
Båge et al., Am J Pathol 2011 (Inflammation...) : In response to tumor necrosis factor ( TNF-a ), IL-1ß, and cocultured lymphocytes, however, mPGES-1 and COX-2 protein expression increased in fibroblasts and smooth muscle cells, accompanied by increased PGE ( 2 ), whereas mPGES-2 and cPGES were unaffected
Hobbs et al., Am J Physiol Gastrointest Liver Physiol 2011 : TNF transactivation of EGFR stimulates cytoprotective COX-2 expression in gastrointestinal epithelial cells ... We hypothesized that COX-2 induction and cell survival signaling downstream of TNF are mediated by EGFR transactivation ... TNF also induced COX-2 protein and mRNA expression in YAMC cells, but blockade of EGFR kinase activity or expression inhibited COX-2 upregulation ... TNF induced COX-2 expression was reduced and absent in EGFR ( -/- ) and TNF receptor-1 (TNFR1) knockout MCE cells, respectively, but was restored upon expression of the WT receptors
Kim et al., J Pharmacol Exp Ther 2011 : Luteolin, a novel natural inhibitor of tumor progression locus 2 serine/threonine kinase, inhibits tumor necrosis factor-alpha induced cyclooxygenase-2 expression in JB6 mouse epidermis cells ... Targeting tumor necrosis factor (TNF)-a mediated signal pathways may be a promising strategy for developing chemopreventive agents, because TNF-a mediated cyclooxygenase (COX)-2 expression plays a key role in inflammation and carcinogenesis ... In the present study, we found that luteolin inhibited TNF-a induced COX-2 expression by down regulating the transactivation of nuclear factor-?B and activator protein-1 ... These effects of luteolin on TNF-a mediated signaling pathways and COX-2 expression are similar to those achieved by blocking tumor progression locus 2 serine/threonine kinase ( TPL2 ) using pharmacologic inhibitors and small interfering RNAs
Park et al., Biochem Pharmacol 2011 (Inflammation...) : Triptolide also abrogated TNF induced expression of cell survival proteins ( XIAP, Bcl-x ( L ), Bcl-2, survivin, cIAP-1 and cIAP-2 ), cell proliferative proteins ( cyclin D1, c-myc and cyclooxygenase-2 ), and metastasis proteins ( ICAM-1 and MMP-9 )
Ferreri et al., Prostaglandins Other Lipid Mediat 2012 : The colocalization of calcium sensing receptor (CaR) and COX-2 in the TAL supports the notion that activation of CaR induces TNF dependent COX-2 expression and PGE2 synthesis in mTAL cells
Seo et al., Immunopharmacol Immunotoxicol 2012 (Inflammation) : SPEE treatment significantly inhibited the LPS induced production of NO, prostaglandin E ( 2 ), interleukin-6, and tumor necrosis factor-a and inhibited the expression of iNOS and COX-2 via attenuation of NF-?B p65 expression
Ma et al., J Pharmacol Sci 2012 : We also demonstrated that pretreatment of cells with this compound prevented TNF-a induced expression of NF-?B target genes, such as interleukin 6, interleukin 8, monocyte chemotactic protein 1, cyclooxygenase-2 and inducible nitric oxide
Piotrowska-Tomala et al., Domest Anim Endocrinol 2012 (Mastitis, Bovine) : Lipopolysaccharides, TNF , IL-1a, and the reagents combination increased PTGS2 , PTGES, and PGFS mRNA transcription ( P < 0.01 ), whereas ALOX5 mRNA transcription was increased only by TNF ( P < 0.001 )
Wang et al., Zhong Xi Yi Jie He Xue Bao 2012 (Adenocarcinoma...) : FYN significantly inhibited TNF-a induced expression of iNOS and COX-2 compared with the control group in A549 cells ( P < 0.01, P < 0.01 ) ... These results suggest that FYN inhibits iNOS and COX-2 activation induced by TNF-a , therefore, it is expected to develop a new strategy to treat lung cancer
Kim et al., Cell Physiol Biochem 2012 (MAP Kinase Signaling System) : The downregulation of IRAK1 inhibited TNF-a induced NF-?B activation and COX-2 expression
Battula et al., Prostaglandins Other Lipid Mediat 2012 (Hypercalcemia...) : Tumor necrosis factor-alpha induces renal cyclooxygenase-2 expression in response to hypercalcemia ... This study demonstrates that COX-2 expression in the OM, secondary to hypercalemia, is TNF dependent
Kim et al., Microsurgery 2012 (Reperfusion Injury) : According to Western blot analysis and a luciferase activity assay, anthocyanins inhibited TNF-a induced intercellular adhesion molecule-1 and cyclooxygenase-2 (COX-2) levels through the NF-?B dependent pathway
Moon et al., Osteoarthritis Cartilage 2013 (Osteoarthritis, Knee) : Resveratrol dose-dependently inhibited TNF-a induced cyclooxygenase-2 (COX-2) , MMP-1, MMP-3, MMP-13 and PGE ( 2 ) production in human chondrocytes
Song et al., Journal of ginseng research 2012 : Air dried PG leaf extract inhibited TNF-a induced NF-?B transcription activity and NF-?B dependent cyclooxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) gene expression more efficiently than the steamed extract ... Furthermore, the ginsenosides Rd and Km inhibited the TNF-a induced expression levels of the COX-2 and iNOS gene in HepG2 cells
Park et al., J Physiol Pharmacol 2013 (Inflammation...) : TNF-a increased the expression of COX-2, iNOS and inflammatory cytokines, but GS-HCl significantly attenuated TNF-a induced COX-2 expression
Akarasereenont et al., Br J Pharmacol 1995 : This study investigates whether endogenous IL-1 beta, TNF-alpha , EGF or PDGF contribute to the induction of COX-2 elicited by LPS in BAEC and if their action is due to activation of tyrosine kinase ... These results suggest that ( i ) the induction of COX activity and COX-2 protein elicited by LPS in BAEC is mediated by TNF-alpha with lesser contributions from PDGF, EGF or IL-1 beta ; ( ii ) exogenous IL-1 beta, TNF-alpha or EGF alone induce COX-2 activity and protein in BAEC ; ( iii ) PDGF synergizes with IL-1 beta, but not TNF-alpha, to cause expression of COX-2 ; and ( iv ) the induction of COX-2 protein and activity caused by these cytokines involves the activation of tyrosine kinase
Feng et al., J Clin Invest 1995 : In addition, NADPH stimulated COX-2 expression, and an inhibitor of NADPH oxidase blocked COX-2 expression induced by TNF alpha
Yamamoto et al., J Biol Chem 1995 : Transcriptional roles of nuclear factor kappa B and nuclear factor-interleukin-6 in the tumor necrosis factor alpha dependent induction of cyclooxygenase-2 in MC3T3-E1 cells ... These data suggested a potential role of both NF-IL6 and NF kappa B in the induction of cyclooxygenase-2 by TNF alpha
Berenbaum et al., Exp Cell Res 1996 : In contrast, TNF increased the effect of IL1 on COX-2 activity and mRNA level
Kawaguchi et al., J Bone Miner Res 1996 (Bone Resorption) : IL-1 and tumor necrosis factor-alpha (TNF-alpha) , both at 1-100 ng/ml, and PTH at 0.1-10 nM increased PGHS-2 and cPLA2 mRNA and medium PGE2 levels dose-dependently after 4 h of treatment ... We conclude that ( 1 ) IL-1, TNF-alpha , and PTH, but not IL-6 nor IL-11, can increase the expression of PGHS-2 , cPLA2, and PGE2 production in cultured mouse calvariae ; ( 2 ) IL-4 inhibits PGE2 production in both control and stimulated calvarial cultures by inhibiting PGHS-2 and cPLA2 ; and ( 3 ) IL-4 has an inhibitory effect on bone resorption which is independent of PG production
Xu et al., J Cell Biochem 1997 : Both TGF beta and TNF could independently increase COX-2 mRNA levels and PG biosynthesis
Belvisi et al., Br J Pharmacol 1997 (Inflammation) : IL-1 beta and TNF alpha increased COX-2 activity and an additional small increase was produced by the three cytokines in combination
Pouliot et al., J Immunol 1997 : However, suppression of PGHS-2 expression is not the result of suppressed cytokine production, because SK & F 86002 suppressed PGHS-2 expression initiated by IL-1beta and TNF-alpha , in addition to other stimuli
Alaaeddine et al., J Rheumatol 1997 (Osteoarthritis) : The TNF-R55 was found to be the major receptor species responsible for ligand binding activity, such as COX-2 induction and PGE2 synthesis, since a specific antireceptor TNF-R55 blocking antibody inhibited about 76 % of TNF-alpha binding and TNF-alpha stimulated COX-2 induction and PGE2 production
Pérez-Sala et al., Biochem Biophys Res Commun 1997 : Supplementing HMC with the BH4 donor sepiapterin potentiated IL-1beta/TNF-alpha induced COX-2 expression by approximately 2-fold
Perkins et al., Prostaglandins 1997 : Tumor necrosis factor-alpha promotes sustained cyclooxygenase-2 expression : attenuation by dexamethasone and NSAIDs ... TNF-alpha stimulated COX-2 expression and the subsequent formation of PGE2 were inhibited by dexamethasone ( 0.1 microM )
Maloney et al., J Immunol 1998 : COX-2 was induced in a time- and concentration dependent fashion when isolated human PMNs were exposed to LPS ; COX-2 was also induced , or its expression was increased, by TNF-alpha , IL-1, and IL-8
Mahboubi et al., Cytokine 1998 : Tumour necrosis factor alpha (TNF-alpha) mediated regulation of prostaglandin endoperoxide synthase-2 ( PGHS-2 ) mRNA levels was examined in murine fibrosarcoma MCA-101 cells ... TNF-alpha induced PGE2 production was evident after 12 h and was associated with a significant TNF-alpha mediated increase in PGHS-2 immunoreactive protein ... TNF-alpha mediated PGHS-2 mRNA accumulation was observed at 1 h, remained elevated for 24 h, and was blocked by actinomycin D, indicating that TNF-alpha increases PGHS-2 gene transcription ... Inhibition of protein tyrosine phosphatases (PTPs) and protein tyrosine kinases ( PTKs ) inhibited the TNF-alpha mediated increase in PGHS-2 mRNA levels ... We suggest that PTPs and PTKs play a role in the transcriptional and/or post-transcriptional mechanisms that contribute to the regulation of the PGHS-2 gene by TNF-alpha
Mahboubi et al., J Pharmacol Exp Ther 1998 : These data demonstrate that tyrosine kinase pathways are not required for TNF-alpha induced NF-kappa B activation in MCA-101 cells and suggest that signaling via these pathways mediates TNF-alpha induced PGHS-2 mRNA accumulation via an NF-kappa B-independent mechanism
Pruzanski et al., Biochim Biophys Acta 1998 : Cox-2 mRNA expression was markedly enhanced by IL-1/TNF and suppressed by all growth factors tested
Morisset et al., J Rheumatol 1998 : Regulation of cyclooxygenase-2 expression in bovine chondrocytes in culture by interleukin 1alpha, tumor necrosis factor-alpha , glucocorticoids, and 17beta-estradiol ... IL-1alpha and TNF-alpha increased the expression of COX-2
Diaz et al., Exp Cell Res 1998 : Analysis of fibroblast proteins showed the induction of cyclooxygenase-1 (Cox-1) by TGF-beta 1 and the induction of Cox-2 by IL-1 beta and TNF-alpha ... In vitro transcription assays showed that IL-1 beta and TNF-alpha increased the transcription of the Cox-2 gene, whereas TGF-beta 1 had no effect ... In summary, we demonstrate that the potentiation of PGE2 production by TGF-beta 1 in IL-1 beta and TNF-alpha treated fibroblasts is the result of transcriptional stimulation of the Cox-2 gene by IL-1 beta and TNF-alpha and the stabilization of the resulting transcripts by TGF-beta 1
Gallois et al., J Biol Chem 1998 : Induction of COX-2 and stimulation of COX activity by ET-1 and TNF-alpha were prevented by sodium salicylate and MG-132, suggesting that activation of NF-kappaB by either factor is needed for stimulation of COX-2
Takahashi et al., Am J Physiol 1998 (Stomach Ulcer) : These results indicate that COX-2 protein is highly localized in the base of gastric ulcers in rats and that COX-2 mRNA expression might be regulated positively by IL-1beta and TNF-alpha and negatively by TGF-beta1
Jobin et al., Immunology 1998 : We used an adenoviral vector containing an NF-kappaB super-repressor ( Ad5IkappaB ) to investigate the role of NF-kappaB in tumour necrosis factor-alpha (TNF-alpha) mediated COX-2 gene expression in a colonic epithelial cell line