◀ Back to PRKDC

PRKDC — XRCC4

Pathways - manually collected, often from reviews:

• NCI Pathway Database DNA-PK pathway in nonhomologous end joining: APLF (APLF) → Ku/Artemis/DNA-PKcs (dimer)/Pol Mu/XRCC4 (dimer)/Ligase IV/APLF complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLM-XRCC4-LIG4-APLF) (modification, collaborate)
  Iles et al., Mol Cell Biol 2007, Kanno et al., EMBO J 2007
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database DNA-PK pathway in nonhomologous end joining: APLF (APLF) → Ku/Artemis/DNA-PKcs (dimer)/Pol Mu/XRCC4 (dimer)/Ligase IV complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLM-XRCC4-LIG4) (modification, collaborate)
  Iles et al., Mol Cell Biol 2007, Kanno et al., EMBO J 2007
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database DNA-PK pathway in nonhomologous end joining: Ku/Artemis/DNA-PKcs (dimer)/Pol Mu/XRCC4 (dimer)/Ligase IV/APLF complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLM-XRCC4-LIG4-APLF) → Ku/Artemis/DNA-PKcs (dimer)/Pol Mu/XRCC4 (dimer)/Ligase IV complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLM-XRCC4-LIG4) (modification, collaborate)
  Iles et al., Mol Cell Biol 2007, Kanno et al., EMBO J 2007
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database DNA-PK pathway in nonhomologous end joining: PNKP (PNKP) → Ku/Artemis/DNA-PKcs (dimer)/Pol Mu/XRCC4 (dimer)/Ligase IV/PNKP complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLM-XRCC4-LIG4-PNKP) (modification, collaborate)
  Koch et al., EMBO J 2004, Williams et al., Biochem Cell Biol 2005
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database DNA-PK pathway in nonhomologous end joining: PNKP (PNKP) → Ku/Artemis/DNA-PKcs (dimer)/Pol Mu/XRCC4 (dimer)/Ligase IV complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLM-XRCC4-LIG4) (modification, collaborate)
  Koch et al., EMBO J 2004, Williams et al., Biochem Cell Biol 2005
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database DNA-PK pathway in nonhomologous end joining: Ku/Artemis/DNA-PKcs (dimer)/Pol Mu/XRCC4 (dimer)/Ligase IV/PNKP complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLM-XRCC4-LIG4-PNKP) → Ku/Artemis/DNA-PKcs (dimer)/Pol Mu/XRCC4 (dimer)/Ligase IV complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLM-XRCC4-LIG4) (modification, collaborate)
  Koch et al., EMBO J 2004, Williams et al., Biochem Cell Biol 2005
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database DNA-PK pathway in nonhomologous end joining: XRCC4 (dimer)/Ligase IV complex (XRCC4-LIG4) → Ku/Artemis/DNA-PKcs (dimer)/Pol Mu/XRCC4 (dimer)/Ligase IV complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLM-XRCC4-LIG4) (modification, collaborate)
  Nick McElhinny et al., Mol Cell Biol 2000, Chen et al., J Biol Chem 2000, Mahajan et al., Mol Cell Biol 2002, Nick McElhinny et al., Mol Cell Biol 2003, Modesti et al., J Mol Biol 2003, Drouet et al., J Biol Chem 2005, Ma et al., Mol Cell 2004, Costantini et al., DNA repair 2007, Gu et al., EMBO J 2007, Li et al., Cell 1995, Grawunder et al., Nature 1997, Leber et al., J Biol Chem 1998
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database DNA-PK pathway in nonhomologous end joining: XRCC4 (dimer)/Ligase IV complex (XRCC4-LIG4) → Ku/Artemis/DNA-PKcs (dimer)/Pol Mu complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLM) (modification, collaborate)
  Nick McElhinny et al., Mol Cell Biol 2000, Chen et al., J Biol Chem 2000, Mahajan et al., Mol Cell Biol 2002, Nick McElhinny et al., Mol Cell Biol 2003, Modesti et al., J Mol Biol 2003, Drouet et al., J Biol Chem 2005, Ma et al., Mol Cell 2004, Costantini et al., DNA repair 2007, Gu et al., EMBO J 2007, Li et al., Cell 1995, Grawunder et al., Nature 1997, Leber et al., J Biol Chem 1998
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database DNA-PK pathway in nonhomologous end joining: Ku/Artemis/DNA-PKcs (dimer)/Pol Mu/XRCC4 (dimer)/Ligase IV complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLM-XRCC4-LIG4) → Ku/Artemis/DNA-PKcs (dimer)/Pol Mu complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLM) (modification, collaborate)
  Nick McElhinny et al., Mol Cell Biol 2000, Chen et al., J Biol Chem 2000, Mahajan et al., Mol Cell Biol 2002, Nick McElhinny et al., Mol Cell Biol 2003, Modesti et al., J Mol Biol 2003, Drouet et al., J Biol Chem 2005, Ma et al., Mol Cell 2004, Costantini et al., DNA repair 2007, Gu et al., EMBO J 2007, Li et al., Cell 1995, Grawunder et al., Nature 1997, Leber et al., J Biol Chem 1998
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database DNA-PK pathway in nonhomologous end joining: XRCC4 (dimer)/Ligase IV complex (XRCC4-LIG4) → Ku/Artemis/DNA-PKcs (dimer)/DNTT complex (XRCC6-XRCC5-DCLRE1C-PRKDC-DNTT) (modification, collaborate)
  Nick McElhinny et al., Mol Cell Biol 2000, Chen et al., J Biol Chem 2000, Mahajan et al., Mol Cell Biol 2002, Modesti et al., J Mol Biol 2003, Drouet et al., J Biol Chem 2005, Costantini et al., DNA repair 2007, Li et al., Cell 1995, Grawunder et al., Nature 1997, Leber et al., J Biol Chem 1998
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database DNA-PK pathway in nonhomologous end joining: XRCC4 (dimer)/Ligase IV complex (XRCC4-LIG4) → Ku/Artemis/DNA-PKcs (dimer)/DNTT/XRCC4 (dimer)/Ligase IV complex (XRCC6-XRCC5-DCLRE1C-PRKDC-DNTT-XRCC4-LIG4) (modification, collaborate)
  Nick McElhinny et al., Mol Cell Biol 2000, Chen et al., J Biol Chem 2000, Mahajan et al., Mol Cell Biol 2002, Modesti et al., J Mol Biol 2003, Drouet et al., J Biol Chem 2005, Costantini et al., DNA repair 2007, Li et al., Cell 1995, Grawunder et al., Nature 1997, Leber et al., J Biol Chem 1998
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database DNA-PK pathway in nonhomologous end joining: Ku/Artemis/DNA-PKcs (dimer)/DNTT complex (XRCC6-XRCC5-DCLRE1C-PRKDC-DNTT) → Ku/Artemis/DNA-PKcs (dimer)/DNTT/XRCC4 (dimer)/Ligase IV complex (XRCC6-XRCC5-DCLRE1C-PRKDC-DNTT-XRCC4-LIG4) (modification, collaborate)
  Nick McElhinny et al., Mol Cell Biol 2000, Chen et al., J Biol Chem 2000, Mahajan et al., Mol Cell Biol 2002, Modesti et al., J Mol Biol 2003, Drouet et al., J Biol Chem 2005, Costantini et al., DNA repair 2007, Li et al., Cell 1995, Grawunder et al., Nature 1997, Leber et al., J Biol Chem 1998
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database DNA-PK pathway in nonhomologous end joining: Aprataxin (APTX) → Ku/Artemis/DNA-PKcs (dimer)/Pol Mu/XRCC4 (dimer)/Ligase IV/Aprataxin complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLM-XRCC4-LIG4-APTX) (modification, collaborate)
  Clements et al., DNA repair 2004, Ahel et al., Nature 2006
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database DNA-PK pathway in nonhomologous end joining: Aprataxin (APTX) → Ku/Artemis/DNA-PKcs (dimer)/Pol Mu/XRCC4 (dimer)/Ligase IV complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLM-XRCC4-LIG4) (modification, collaborate)
  Clements et al., DNA repair 2004, Ahel et al., Nature 2006
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database DNA-PK pathway in nonhomologous end joining: Ku/Artemis/DNA-PKcs (dimer)/Pol Mu/XRCC4 (dimer)/Ligase IV/Aprataxin complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLM-XRCC4-LIG4-APTX) → Ku/Artemis/DNA-PKcs (dimer)/Pol Mu/XRCC4 (dimer)/Ligase IV complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLM-XRCC4-LIG4) (modification, collaborate)
  Clements et al., DNA repair 2004, Ahel et al., Nature 2006
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database DNA-PK pathway in nonhomologous end joining: 0005956 () → Ku/Artemis/DNA-PKcs (dimer)/Pol Mu/XRCC4 (dimer)/Ligase IV complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLM-XRCC4-LIG4) (modification, activates)
  Clements et al., DNA repair 2004, Koch et al., EMBO J 2004, Iles et al., Mol Cell Biol 2007, Kanno et al., EMBO J 2007
  Evidence: mutant phenotype, assay
• NCI Pathway Database DNA-PK pathway in nonhomologous end joining: Ku/Artemis/DNA-PKcs (dimer)/Pol Mu/XRCC4 (dimer)/Ligase IV/XLF (dimer) complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLM-XRCC4-LIG4-NHEJ1) → XLF (dimer) complex (NHEJ1) (modification, collaborate)
  Ahnesorg et al., Cell 2006, Callebaut et al., J Biol Chem 2006, Tsai et al., Proc Natl Acad Sci U S A 2007, Gu et al., Nucleic Acids Res 2007, Li et al., EMBO J 2008, Andres et al., Mol Cell 2007
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database DNA-PK pathway in nonhomologous end joining: Ku/Artemis/DNA-PKcs (dimer)/Pol Mu/XRCC4 (dimer)/Ligase IV/XLF (dimer) complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLM-XRCC4-LIG4-NHEJ1) → Ku/Artemis/DNA-PKcs (dimer)/Pol Mu/XRCC4 (dimer)/Ligase IV complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLM-XRCC4-LIG4) (modification, collaborate)
  Ahnesorg et al., Cell 2006, Callebaut et al., J Biol Chem 2006, Tsai et al., Proc Natl Acad Sci U S A 2007, Gu et al., Nucleic Acids Res 2007, Li et al., EMBO J 2008, Andres et al., Mol Cell 2007
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database DNA-PK pathway in nonhomologous end joining: XLF (dimer) complex (NHEJ1) → Ku/Artemis/DNA-PKcs (dimer)/Pol Mu/XRCC4 (dimer)/Ligase IV complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLM-XRCC4-LIG4) (modification, collaborate)
  Ahnesorg et al., Cell 2006, Callebaut et al., J Biol Chem 2006, Tsai et al., Proc Natl Acad Sci U S A 2007, Gu et al., Nucleic Acids Res 2007, Li et al., EMBO J 2008, Andres et al., Mol Cell 2007
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database DNA-PK pathway in nonhomologous end joining: Ku/Artemis/DNA-PKcs (dimer)/POLL complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLL) → XRCC4 (dimer)/Ligase IV complex (XRCC4-LIG4) (modification, collaborate)
  Nick McElhinny et al., Mol Cell Biol 2000, Chen et al., J Biol Chem 2000, Lee et al., J Biol Chem 2004, Modesti et al., J Mol Biol 2003, Drouet et al., J Biol Chem 2005, Ma et al., Mol Cell 2004, Costantini et al., DNA repair 2007, Gu et al., EMBO J 2007, Li et al., Cell 1995, Grawunder et al., Nature 1997, Leber et al., J Biol Chem 1998
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database DNA-PK pathway in nonhomologous end joining: Ku/Artemis/DNA-PKcs (dimer)/POLL complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLL) → Ku/Artemis/DNA-PKcs (dimer)/POLL/XRCC4 (dimer)/Ligase IV complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLL-XRCC4-LIG4) (modification, collaborate)
  Nick McElhinny et al., Mol Cell Biol 2000, Chen et al., J Biol Chem 2000, Lee et al., J Biol Chem 2004, Modesti et al., J Mol Biol 2003, Drouet et al., J Biol Chem 2005, Ma et al., Mol Cell 2004, Costantini et al., DNA repair 2007, Gu et al., EMBO J 2007, Li et al., Cell 1995, Grawunder et al., Nature 1997, Leber et al., J Biol Chem 1998
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database DNA-PK pathway in nonhomologous end joining: XRCC4 (dimer)/Ligase IV complex (XRCC4-LIG4) → Ku/Artemis/DNA-PKcs (dimer)/POLL/XRCC4 (dimer)/Ligase IV complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLL-XRCC4-LIG4) (modification, collaborate)
  Nick McElhinny et al., Mol Cell Biol 2000, Chen et al., J Biol Chem 2000, Lee et al., J Biol Chem 2004, Modesti et al., J Mol Biol 2003, Drouet et al., J Biol Chem 2005, Ma et al., Mol Cell 2004, Costantini et al., DNA repair 2007, Gu et al., EMBO J 2007, Li et al., Cell 1995, Grawunder et al., Nature 1997, Leber et al., J Biol Chem 1998
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database DNA-PK pathway in nonhomologous end joining: XLF (dimer) complex (NHEJ1) → Ku/Artemis/DNA-PKcs (dimer)/POLL/XRCC4 (dimer)/Ligase IV/XLF (dimer) complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLL-XRCC4-LIG4-NHEJ1) (modification, collaborate)
  Ahnesorg et al., Cell 2006, Callebaut et al., J Biol Chem 2006, Tsai et al., Proc Natl Acad Sci U S A 2007, Gu et al., Nucleic Acids Res 2007, Li et al., EMBO J 2008, Andres et al., Mol Cell 2007
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database DNA-PK pathway in nonhomologous end joining: XLF (dimer) complex (NHEJ1) → Ku/Artemis/DNA-PKcs (dimer)/POLL/XRCC4 (dimer)/Ligase IV complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLL-XRCC4-LIG4) (modification, collaborate)
  Ahnesorg et al., Cell 2006, Callebaut et al., J Biol Chem 2006, Tsai et al., Proc Natl Acad Sci U S A 2007, Gu et al., Nucleic Acids Res 2007, Li et al., EMBO J 2008, Andres et al., Mol Cell 2007
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database DNA-PK pathway in nonhomologous end joining: Ku/Artemis/DNA-PKcs (dimer)/POLL/XRCC4 (dimer)/Ligase IV/XLF (dimer) complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLL-XRCC4-LIG4-NHEJ1) → Ku/Artemis/DNA-PKcs (dimer)/POLL/XRCC4 (dimer)/Ligase IV complex (XRCC6-XRCC5-DCLRE1C-PRKDC-POLL-XRCC4-LIG4) (modification, collaborate)
  Ahnesorg et al., Cell 2006, Callebaut et al., J Biol Chem 2006, Tsai et al., Proc Natl Acad Sci U S A 2007, Gu et al., Nucleic Acids Res 2007, Li et al., EMBO J 2008, Andres et al., Mol Cell 2007
  Evidence: mutant phenotype, assay, physical interaction
• Reactome Reaction: PRKDC → XRCC4 (reaction) Kurimasa et al., Mol Cell Biol 1999, Kienker et al., Nucleic Acids Res 2000, Douglas et al., J Biol Chem 2001, Douglas et al., Biochem J 2002, Chan et al., Genes Dev 2002, Merkle et al., Biochemistry 2002, Ding et al., Mol Cell Biol 2003, Chan et al., J Biol Chem 1996, Critchlow et al., Curr Biol 1997*, Leber et al., J Biol Chem 1998, Baumann et al., Proc Natl Acad Sci U S A 1998
• Reactome Reaction: PRKDC → XRCC4 (direct_complex) Critchlow et al., Curr Biol 1997*, Leber et al., J Biol Chem 1998

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

• IRef Biogrid Interaction: PRKDC — XRCC4 (colocalization, biochemical) Havugimana et al., Cell 2012
• IRef Biogrid Interaction: PRKDC — XRCC4 (physical association, affinity chromatography technology) Wang et al., J Biol Chem 2004*
• IRef Biogrid Interaction: PRKDC — XRCC4 (physical association, affinity chromatography technology) Drouet et al., J Biol Chem 2005
• Gene Ontology Complexes nonhomologous end joining complex: nonhomologous end joining complex complex (LIG4-PRKDC-XRCC5-XRCC4-XRCC6) Roberts et al., Nature 2010*
• Gene Ontology Complexes DNA-dependent protein kinase-DNA ligase 4 complex: DNA-dependent protein kinase-DNA ligase 4 complex complex (LIG4-XRCC4-XRCC6BP1-PRKDC) Yang et al., Nucleic Acids Res 1999*, Wang et al., J Biol Chem 2004*
• IRef Hprd Interaction: XRCC4 — PRKDC (in vitro) Leber et al., J Biol Chem 1998

Text-mined interactions from Literome

Lee et al., DNA repair 2004 : Results demonstrate that DNA-PKcs mediated phosphorylation of XRCC4 serine 260 and serine 318 does not directly control end joining under the conditions tested
Yano et al., Cell cycle (Georgetown, Tex.) 2008 : XRCC4 can be recruited to DSBs without DNA-PKcs, but DNA-PKcs stabilizes the recruited XRCC4
Leber et al., J Biol Chem 1998 : Both DNA-PK dependent and independent phosphorylation of XRCC4 in vitro occurs only on serine and threonine residues within the COOH-terminal 130 amino acids, a region of the molecule that is not absolutely required for XRCC4 's DSBR function