Immunity 1997,
PMID: 9133424
Takeshita, T; Arita, T; Higuchi, M; Asao, H; Endo, K; Kuroda, H; Tanaka, N; Murata, K; Ishii, N; Sugamura, K
We previously identified a putative signal transducing adaptor molecule, named STAM, that contains an Src homology 3 (SH3) domain and immunoreceptor tyrosine-based activation motif (ITAM). In this report, we demonstrate the functional significance of STAM in cytokine-mediated signal transduction. STAM is associated with Jak3 and Jak2 tyrosine kinases via its ITAM region and phosphorylated by Jak3 and Jak2 upon stimulation with IL-2 and GM-CSF, respectively. An SH3 deletion mutant of STAM confers a dominant-negative effect on DNA synthesis mediated by IL-2 and GM-CSF. Furthermore, the wild-type STAM, but not STAM mutants deleted of SH3 and ITAM, significantly enhances c-myc induction mediated by IL-2 and GM-CSF. These results strongly implicate STAM in the signaling pathways for cell growth and c-myc induction immediately downstream of the Jaks associated with the cytokine receptors.
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Text Mining Data
STAM → GM-CSF: "
STAM is associated with Jak3 and Jak2 tyrosine kinases via its ITAM region and phosphorylated by Jak3 and Jak2 upon
stimulation with IL-2 and
GM-CSF , respectively
"
STAM → IL-2: "
STAM is associated with Jak3 and Jak2 tyrosine kinases via its ITAM region and phosphorylated by Jak3 and Jak2 upon stimulation with IL-2 and GM-CSF, respectively
"
Jak2 → GM-CSF: "
STAM is associated with Jak3 and Jak2 tyrosine kinases via its ITAM region and phosphorylated by Jak3 and Jak2 upon stimulation with IL-2 and GM-CSF , respectively
"
Jak2 → IL-2: "
STAM is associated with Jak3 and Jak2 tyrosine kinases via its ITAM region and phosphorylated by Jak3 and Jak2 upon stimulation with IL-2 and GM-CSF, respectively
"
Jak3 → GM-CSF: "
STAM is associated with Jak3 and Jak2 tyrosine kinases via its ITAM region and phosphorylated by Jak3 and Jak2 upon stimulation with IL-2 and GM-CSF , respectively
"
Jak3 → IL-2: "
STAM is associated with Jak3 and Jak2 tyrosine kinases via its ITAM region and phosphorylated by Jak3 and Jak2 upon stimulation with IL-2 and GM-CSF, respectively
"
Manually curated Databases
-
IRef Biogrid Interaction:
STAM
—
JAK3
(physical association, affinity chromatography technology)
-
IRef Biogrid Interaction:
STAM
—
JAK2
(direct interaction, enzymatic study)
-
IRef Biogrid Interaction:
STAM
—
JAK2
(physical association, affinity chromatography technology)
-
IRef Hprd Interaction:
STAM
—
JAK2
(in vivo)
-
IRef Hprd Interaction:
STAM
—
JAK1
(in vivo)
-
IRef Ophid Interaction:
STAM
—
JAK2
(aggregation, confirmational text mining)
-
NCI Pathway Database IL2-mediated signaling events:
IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/STAM1-2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-STAM2_STAM)
→
IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3)
(modification, collaborate)
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database IL2-mediated signaling events:
IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/STAM1-2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-STAM2_STAM)
→
STAM1-2 (STAM2/STAM)
(modification, collaborate)
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database IL2-mediated signaling events:
IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3)
→
STAM1-2 (STAM2/STAM)
(modification, collaborate)
Evidence: mutant phenotype, physical interaction
In total, 46 gene pairs are associated to this article in curated databases