Phytochemistry 1996,
PMID: 8722085
Biswas, T K; Promo, M; Biswas, B
Acid phosphatase I (AP-I) is the major isoform of Vigna acid phosphatase. It is constitutively expressed in seed cotyledons during germination. AP-I was separated from other isoforms and purified to homogeneity by three simple purification steps; (NH4)2SO4 precipitation, and phosphocellulose and DEAE-cellulose column chromatography. The activity of AP-I was not affected by 1 mM Mg2+, Mn2+, Ca2+, Co2+ or Pb2+, but severely inhibited by 1 mM Cu2+, Fe3+, Hg2+, Mo6+ or Zn2+. AP-I has both phosphatase and pyrophosphatase activities, and is highly stable even at 50 degrees.
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Text Mining Data
AP-I — Mg2+: "
The activity of
AP-I was not
affected by 1 mM
Mg2+ , Mn2+, Ca2+, Co2+ or Pb2+, but severely inhibited by 1 mM Cu2+, Fe3+, Hg2+, Mo6+ or Zn2+
"
AP-I — Ca2+: "
The activity of AP-I was not affected by 1 mM Mg2+, Mn2+, Ca2+ , Co2+ or Pb2+, but severely inhibited by 1 mM Cu2+, Fe3+, Hg2+, Mo6+ or Zn2+
"
Manually curated Databases
No curated data.