Biomed Environ Sci 2011,
PMID: 22108332
Jia, HongLing; Liu, ChaoWu; Ge, Feng; Xiao, ChuanLe; Lu, ChunHua; Wang, Tong; He, QingYu
OBJECTIVE
To identify ubiquitinated proteins from complex human multiple myeloma (MM) U266 cells, a malignant disorder of differentiated human B cells.
METHODS
Employing a globally proteomic strategy combining of immunoprecipitation, LC-MS/MS and SCX-LC-MS analysis to identified ubiquitination sites, which were identified by detecting signature peptides containing a GG-tag (114.1 Da) and an LRGG-tag (383.2 Da).
RESULTS
In total, 52 ubiquitinated proteins containing 73 ubiquitination sites of which 14 and 59 sites contained LRGG-tag and GG-tag were identified, respectively.
CONCLUSIONS
Classification analysis by of the proteins identified in the study based on the PANTHER showed that they were associated with multiple functional groups. This suggested the involvement of many endogenous proteins in the ubiquitination in MM.
Diseases/Pathways annotated by Medline MESH: Multiple Myeloma
Document information provided by NCBI PubMed
Text Mining Data
Dashed line = No text mining data
Manually curated Databases
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IRef Biogrid Interaction:
RPS27A
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
UBC
—
HNRNPH1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
UBC
—
MCM7
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MSH6
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
UBC
—
CLTC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HNRNPA3
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
UBC
—
RPLP0
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
TUBB4B
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
UBC
—
NONO
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
UBC
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ALDOA
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
UBC
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PABPC4
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
UBC
—
POLR2A
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
UBC
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RRBP1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
PDCD11
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
YLPM1
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
SNRNP200
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
UBC
—
FLNC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
DDX21
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
BOD1L1
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
UBC
—
EIF4G1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
TUBA1B
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
UBC
—
FXR1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
DIEXF
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
UBC
—
NUP210
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
PRRC2C
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
UTP20
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
UBC
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DYNC1H1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
UBC
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MYL4
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
EIF5B
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
EIF3A
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
PSMD3
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
XRCC6
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HSPA2
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
VCP
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
DHX9
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
TUBA1C
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
UBC
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EIF3H
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MDC1
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
UBC
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SPTBN1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HNRNPD
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HUWE1
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
GCN1L1
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
EEF1A1
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
TUBB2A
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
PSMC6
—
UBC
(physical association, affinity chromatography technology)
In total, 45 gene pairs are associated to this article in curated databases