Gene interactions and pathways from curated databases and text-mining
Biomed Environ Sci 2011, PMID: 22108332

Identification of ubiquitinated proteins from human multiple myeloma U266 cells by proteomics.

Jia, HongLing; Liu, ChaoWu; Ge, Feng; Xiao, ChuanLe; Lu, ChunHua; Wang, Tong; He, QingYu

OBJECTIVE

To identify ubiquitinated proteins from complex human multiple myeloma (MM) U266 cells, a malignant disorder of differentiated human B cells.

METHODS

Employing a globally proteomic strategy combining of immunoprecipitation, LC-MS/MS and SCX-LC-MS analysis to identified ubiquitination sites, which were identified by detecting signature peptides containing a GG-tag (114.1 Da) and an LRGG-tag (383.2 Da).

RESULTS

In total, 52 ubiquitinated proteins containing 73 ubiquitination sites of which 14 and 59 sites contained LRGG-tag and GG-tag were identified, respectively.

CONCLUSIONS

Classification analysis by of the proteins identified in the study based on the PANTHER showed that they were associated with multiple functional groups. This suggested the involvement of many endogenous proteins in the ubiquitination in MM.

Diseases/Pathways annotated by Medline MESH: Multiple Myeloma
Document information provided by NCBI PubMed

Text Mining Data

Dashed line = No text mining data

Manually curated Databases

  • IRef Biogrid Interaction: RPS27A — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UBC — HNRNPH1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UBC — MCM7 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MSH6 — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UBC — CLTC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HNRNPA3 — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UBC — RPLP0 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TUBB4B — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UBC — NONO (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UBC — ALDOA (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UBC — PABPC4 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UBC — POLR2A (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UBC — RRBP1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PDCD11 — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: YLPM1 — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SNRNP200 — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UBC — FLNC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DDX21 — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: BOD1L1 — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UBC — EIF4G1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TUBA1B — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UBC — FXR1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DIEXF — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UBC — NUP210 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PRRC2C — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UTP20 — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UBC — DYNC1H1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UBC — MYL4 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EIF5B — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EIF3A — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PSMD3 — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: XRCC6 — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HSPA2 — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: VCP — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DHX9 — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TUBA1C — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UBC — EIF3H (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MDC1 — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UBC — SPTBN1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HNRNPD — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HUWE1 — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: GCN1L1 — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EEF1A1 — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TUBB2A — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PSMC6 — UBC (physical association, affinity chromatography technology)
In total, 45 gene pairs are associated to this article in curated databases