J Cell Sci 2007,
PMID: 17502486
Bryant, David M; Kerr, Markus C; Hammond, Luke A; Joseph, Shannon R; Mostov, Keith E; Teasdale, Rohan D; Stow, Jennifer L
In epithelia, junction proteins are endocytosed for modulation of cell-cell adhesion and cell polarity. In response to growth factors, the cell-cell adhesion protein E-cadherin is internalized from the cell surface with degradation or recycling as potential fates. However, the cellular machinery involved in cadherin internalization and recycling remains controversial. Here we investigated EGF-induced E-cadherin internalization. EGF stimulation of MCF-7 cells resulted in Rac1-modulated macropinocytosis of the E-cadherin-catenin complex into endosomal compartments that colocalized with EEA1 and the sorting nexin, SNX1. Depletion of cellular SNX1 levels by siRNA resulted in increased intracellular accumulation and turnover of E-cadherin internalized from the cell surface in response to EGF. Moreover, SNX1 was also required for efficient recycling of internalized E-cadherin and re-establishment of epithelial adhesion. Together, these findings demonstrate a role for SNX1 in retrieval of E-cadherin from a degradative endosomal pathway and in membrane trafficking pathways that regulate E-cadherin recycling.
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Text Mining Data
Dashed line = No text mining data
Manually curated Databases
-
NCI Pathway Database Posttranslational regulation of adherens junction stability and dissassembly:
SNX1 (SNX1)
→
E-cadherin/beta catenin/p120 catenin complex (CDH1-CTNNB1-CTNND1)
(translocation, activates)
Evidence: other species
-
NCI Pathway Database Posttranslational regulation of adherens junction stability and dissassembly:
None
→
alpha catenin (CTNNA1)
(translocation, collaborate)
Evidence: mutant phenotype, other species
-
NCI Pathway Database Posttranslational regulation of adherens junction stability and dissassembly:
None
→
E-cadherin/beta catenin/p120 catenin complex (CDH1-CTNNB1-CTNND1)
(translocation, collaborate)
Evidence: mutant phenotype, other species
-
NCI Pathway Database Posttranslational regulation of adherens junction stability and dissassembly:
None
→
E-cadherin/Ca2+/beta catenin/alpha catenin/p120 catenin complex (CDH1-CTNNB1-CTNNA1-CTNND1)
(translocation, collaborate)
Evidence: mutant phenotype, other species
-
NCI Pathway Database Posttranslational regulation of adherens junction stability and dissassembly:
alpha catenin (CTNNA1)
→
E-cadherin/beta catenin/p120 catenin complex (CDH1-CTNNB1-CTNND1)
(translocation, collaborate)
Evidence: mutant phenotype, other species
-
NCI Pathway Database Posttranslational regulation of adherens junction stability and dissassembly:
alpha catenin (CTNNA1)
→
E-cadherin/Ca2+/beta catenin/alpha catenin/p120 catenin complex (CDH1-CTNNB1-CTNNA1-CTNND1)
(translocation, collaborate)
Evidence: mutant phenotype, other species
-
NCI Pathway Database Posttranslational regulation of adherens junction stability and dissassembly:
E-cadherin/beta catenin/p120 catenin complex (CDH1-CTNNB1-CTNND1)
→
E-cadherin/Ca2+/beta catenin/alpha catenin/p120 catenin complex (CDH1-CTNNB1-CTNNA1-CTNND1)
(translocation, collaborate)
Evidence: mutant phenotype, other species
-
NCI Pathway Database Posttranslational regulation of adherens junction stability and dissassembly:
RAC1/GTP complex (RAC1)
→
E-cadherin/Ca2+/beta catenin/alpha catenin/p120 catenin complex (CDH1-CTNNB1-CTNNA1-CTNND1)
(translocation, activates)
Evidence: mutant phenotype, other species
-
NCI Pathway Database Posttranslational regulation of adherens junction stability and dissassembly:
None
→
RAC1/GDP complex (RAC1)
(modification, collaborate)
Evidence: mutant phenotype, other species
-
NCI Pathway Database Posttranslational regulation of adherens junction stability and dissassembly:
None
→
RAC1/GTP complex (RAC1)
(modification, collaborate)
Evidence: mutant phenotype, other species
-
NCI Pathway Database Posttranslational regulation of adherens junction stability and dissassembly:
None
→
EGFR/EGFR/EGF/EGF complex (EGFR-EGF)
(modification, collaborate)
Evidence: mutant phenotype, other species
-
NCI Pathway Database Posttranslational regulation of adherens junction stability and dissassembly:
None
→
None
(modification, collaborate)
Evidence: mutant phenotype, other species
-
NCI Pathway Database Posttranslational regulation of adherens junction stability and dissassembly:
RAC1/GDP complex (RAC1)
→
RAC1/GTP complex (RAC1)
(modification, collaborate)
Evidence: mutant phenotype, other species
-
NCI Pathway Database Posttranslational regulation of adherens junction stability and dissassembly:
RAC1/GDP complex (RAC1)
→
EGFR/EGFR/EGF/EGF complex (EGFR-EGF)
(modification, collaborate)
Evidence: mutant phenotype, other species
-
NCI Pathway Database Posttranslational regulation of adherens junction stability and dissassembly:
RAC1/GDP complex (RAC1)
→
None
(modification, collaborate)
Evidence: mutant phenotype, other species
-
NCI Pathway Database Posttranslational regulation of adherens junction stability and dissassembly:
RAC1/GTP complex (RAC1)
→
None
(modification, collaborate)
Evidence: mutant phenotype, other species
-
NCI Pathway Database Posttranslational regulation of adherens junction stability and dissassembly:
EGFR/EGFR/EGF/EGF complex (EGFR-EGF)
→
None
(modification, activates)
Evidence: mutant phenotype, other species
In total, 21 gene pairs are associated to this article in curated databases