Science 2007,
PMID: 17289999
Willis, Simon N; Fletcher, Jamie I; Kaufmann, Thomas; van Delft, Mark F; Chen, Lin; Czabotar, Peter E; Ierino, Helen; Lee, Erinna F; Fairlie, W Douglas; Bouillet, Philippe; Strasser, Andreas; Kluck, Ruth M; Adams, Jerry M; Huang, David C S
A central issue in the regulation of apoptosis by the Bcl-2 family is whether its BH3-only members initiate apoptosis by directly binding to the essential cell-death mediators Bax and Bak, or whether they can act indirectly, by engaging their pro-survival Bcl-2-like relatives. Contrary to the direct-activation model, we show that Bax and Bak can mediate apoptosis without discernable association with the putative BH3-only activators (Bim, Bid, and Puma), even in cells with no Bim or Bid and reduced Puma. Our results indicate that BH3-only proteins induce apoptosis at least primarily by engaging the multiple pro-survival relatives guarding Bax and Bak.
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Text Mining Data
Dashed line = No text mining data
Manually curated Databases
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IRef Intact Interaction:
BCL2
—
BCL2L11
(physical association, anti tag coimmunoprecipitation)
-
IRef Intact Interaction:
BCL2
—
BBC3
(physical association, anti tag coimmunoprecipitation)
-
IRef Intact Interaction:
Complex of BAX-BCL2L11
(physical association, surface plasmon resonance)
-
IRef Intact Interaction:
Complex of BID-BID-BAX
(physical association, surface plasmon resonance)
-
IRef Intact Interaction:
Complex of BCL2L1-BCL2-BCL2L2-BCL2L2-PABPN1-BAX-BID
(association, anti tag coimmunoprecipitation)
-
IRef Intact Interaction:
Complex of BAX-BAX-BID-BID
(physical association, surface plasmon resonance)
-
IRef Intact Interaction:
Complex of BID-BAX-BID-BCL2-BAX-BAX-BCL2-BCL2-BID
(association, anti tag coimmunoprecipitation)
-
IRef Intact Interaction:
Complex of BID-BAX-BID-BAX
(physical association, surface plasmon resonance)
-
IRef Intact Interaction:
Complex of BAX-BCL2L2-BCL2L2-PABPN1-BAX-BID-BID-BCL2L2-BCL2L2-PABPN1
(physical association, surface plasmon resonance)
-
IRef Intact Interaction:
Complex of BAK1-BAX-BAX
(association, anti tag coimmunoprecipitation)
-
IRef Intact Interaction:
Complex of BCL2L11-BAX
(physical association, surface plasmon resonance)
-
IRef Intact Interaction:
Complex of BAX-BAK1-BAX-BCL2
(association, anti tag coimmunoprecipitation)
-
IRef Intact Interaction:
Complex of BAX-BID-BCL2L1-BCL2L1-BAX-BID
(physical association, surface plasmon resonance)
-
IRef Intact Interaction:
Complex of BCL2L1-BAX-BCL2-BCL2L11-BCL2L2-BCL2L2-PABPN1
(association, anti tag coimmunoprecipitation)
-
IRef Intact Interaction:
BAX
—
BCL2L11
(direct interaction, surface plasmon resonance)
-
IRef Intact Interaction:
BMF
—
BCL2
(physical association, anti tag coimmunoprecipitation)
-
IRef Intact Interaction:
BAX
—
BID
(direct interaction, surface plasmon resonance)
-
IRef Intact Interaction:
BAX
—
BCL2L1
(physical association, anti tag coimmunoprecipitation)
-
IRef Intact Interaction:
BAX
—
BCL2
(physical association, anti tag coimmunoprecipitation)
-
IRef Intact Interaction:
BID
—
BCL2
(physical association, anti tag coimmunoprecipitation)
-
IRef Intact Interaction:
BAX
—
BCL2L2, BCL2L2-PABPN1
(physical association, anti tag coimmunoprecipitation)
-
IRef Intact Interaction:
BIK
—
BCL2
(physical association, anti tag coimmunoprecipitation)
-
IRef Intact Interaction:
Complex of BCL2-BCL2L11-BAX
(association, anti tag coimmunoprecipitation)
-
IRef Intact Interaction:
Complex of BCL2L11-BCL2-BAX
(association, anti tag coimmunoprecipitation)
-
IRef Intact Interaction:
Complex of BCL2L11-BCL2-BAX
(association, anti tag coimmunoprecipitation)
-
IRef Intact Interaction:
Complex of BAX-BCL2L11-BCL2
(association, anti tag coimmunoprecipitation)
In total, 25 gene pairs are associated to this article in curated databases