Gene interactions and pathways from curated databases and text-mining
J Biomed Sci 2004, PMID: 15591777

Heme oxygenase-1 attenuates interleukin-1beta-induced nitric oxide synthase expression in vascular smooth muscle cells.

Yang, Nian-Chung; Lu, Liang-Huei; Kao, Yung-Hsi; Chau, Lee-Young

Heme oxygenase-1 (HO-1) has been implicated in antioxidant and anti-inflammatory actions. To characterize the role of HO-1 in the vascular inflammatory response, we examined the effect of HO-1 on the expression of inducible nitric oxide synthase (iNOS) induced by interleukin-1beta (IL-1beta) in rat vascular smooth muscle cells (VSMCs). Western blot analysis demonstrated that IL-1beta-induced iNOS expression was significantly reduced by hemin cotreatment or adenovirus-mediated HO-1 gene transfer. Scavenging carbon monoxide (CO), one of the by-products of heme degradation by HO-1, significantly attenuated HO-1-mediated suppression of iNOS gene induction as revealed by Northern blot analysis. Exposure of cells to CO or a CO donor, the tricarbonyldichlororuthenium(II) dimer, also markedly inhibited IL-1beta-induced iNOS expression. Transient transfection experiments with a reporter gene construct carrying the rat iNOS gene promoter demonstrated that IL-1beta-induced promoter activity was substantially reduced by cotreatment with CO or a CO donor. Furthermore, the effects of CO on iNOS gene promoter activity and protein expression were diminished by cotreatment with the specific guanylate cyclase inhibitor, 1H-[1,2,4]oxadiazolo-(4,3-a)quinoxalin-1-one. These data support the finding that HO-1 attenuates IL-1beta-induced iNOS gene expression in VSMCs. CO appears to mediate the suppressive effect of HO-1, at least in part, through downregulating transcriptional activation of the iNOS gene via a cGMP-dependent pathway.

Document information provided by NCBI PubMed

Text Mining Data

nitric oxide synthase ⊣ Heme oxygenase-1: " Heme oxygenase-1 attenuates interleukin-1beta induced nitric oxide synthase expression in vascular smooth muscle cells "

nitric oxide synthase → interleukin-1beta: " Heme oxygenase-1 attenuates interleukin-1beta induced nitric oxide synthase expression in vascular smooth muscle cells "

inducible nitric oxide synthase (iNOS) → interleukin-1beta (IL-1beta): " To characterize the role of HO-1 in the vascular inflammatory response, we examined the effect of HO-1 on the expression of inducible nitric oxide synthase (iNOS) induced by interleukin-1beta (IL-1beta) in rat vascular smooth muscle cells ( VSMCs ) "

inducible nitric oxide synthase (iNOS) — HO-1: " To characterize the role of HO-1 in the vascular inflammatory response, we examined the effect of HO-1 on the expression of inducible nitric oxide synthase (iNOS) induced by interleukin-1beta (IL-1beta) in rat vascular smooth muscle cells ( VSMCs ) "

iNOS ⊣ HO-1: " Western blot analysis demonstrated that IL-1beta induced iNOS expression was significantly reduced by hemin cotreatment or adenovirus mediated HO-1 gene transfer "

iNOS → IL-1beta: " Western blot analysis demonstrated that IL-1beta induced iNOS expression was significantly reduced by hemin cotreatment or adenovirus mediated HO-1 gene transfer "

iNOS ⊣ HO-1: " Scavenging carbon monoxide ( CO ), one of the by-products of heme degradation by HO-1, significantly attenuated HO-1 mediated suppression of iNOS gene induction as revealed by Northern blot analysis "

iNOS → IL-1beta: " Exposure of cells to CO or a CO donor, the tricarbonyldichlororuthenium ( II ) dimer, also markedly inhibited IL-1beta induced iNOS expression "

iNOS → IL-1beta: " These data support the finding that HO-1 attenuates IL-1beta induced iNOS gene expression in VSMCs. CO appears to mediate the suppressive effect of HO-1, at least in part, through downregulating transcriptional activation of the iNOS gene via a cGMP dependent pathway "

Manually curated Databases

No curated data.