Mouse Gene Slc25a20 (ENSMUST00000035222.6) from GENCODE VM33
  Description: Mus musculus solute carrier family 25 (mitochondrial carnitine/acylcarnitine translocase), member 20 (Slc25a20), mRNA; nuclear gene for mitochondrial product. (from RefSeq NM_020520)
Gencode Transcript: ENSMUST00000035222.6
Gencode Gene: ENSMUSG00000032602.7
Transcript (Including UTRs)
   Position: mm39 chr9:108,539,297-108,561,840 Size: 22,544 Total Exon Count: 9 Strand: +
Coding Region
   Position: mm39 chr9:108,539,427-108,561,093 Size: 21,667 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Methods
Data last updated at UCSC: 2023-08-16 04:06:27

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr9:108,539,297-108,561,840)mRNA (may differ from genome)Protein (301 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedReactomeSOURCEUniProtKB

-  Comments and Description Text from UniProtKB
  ID: MCAT_MOUSE
DESCRIPTION: RecName: Full=Mitochondrial carnitine/acylcarnitine carrier protein; AltName: Full=Carnitine/acylcarnitine translocase; Short=CAC; Short=mCAC; AltName: Full=Solute carrier family 25 member 20;
FUNCTION: Mediates the transport of acylcarnitines of different length across the mitochondrial inner membrane from the cytosol to the mitochondrial matrix for their oxidation by the mitochondrial fatty acid-oxidation pathway (By similarity).
SUBCELLULAR LOCATION: Mitochondrion inner membrane; Multi-pass membrane protein (By similarity).
SIMILARITY: Belongs to the mitochondrial carrier family.
SIMILARITY: Contains 3 Solcar repeats.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -51.20130-0.394 Picture PostScript Text
3' UTR -224.10747-0.300 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR018108 - Mitochondrial_sb/sol_carrier
IPR023395 - Mt_carrier_dom

Pfam Domains:
PF00153 - Mitochondrial carrier protein

ModBase Predicted Comparative 3D Structure on Q9Z2Z6
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserGenome BrowserNo orthologGenome Browser
Gene Details    Gene Details
Gene Sorter    Gene Sorter
GenBankRGDEnsemblEnsembl SGD
Protein SequenceProtein SequenceProtein SequenceProtein Sequence Protein Sequence
AlignmentAlignmentAlignmentAlignment Alignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005476 carnitine:acyl carnitine antiporter activity
GO:0015226 carnitine transmembrane transporter activity
GO:0015227 acyl carnitine transmembrane transporter activity

Biological Process:
GO:0006839 mitochondrial transport
GO:0015879 carnitine transport
GO:1902603 carnitine transmembrane transport
GO:1902616 acyl carnitine transmembrane transport

Cellular Component:
GO:0005739 mitochondrion
GO:0005743 mitochondrial inner membrane
GO:0005829 cytosol
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  AK161086 - Mus musculus 10 days neonate skin cDNA, RIKEN full-length enriched library, clone:4732409K14 product:solute carrier family 25 (mitochondrial carnitine/acylcarnitine translocase), member 20, full insert sequence.
AK075624 - Mus musculus 18-day embryo whole body cDNA, RIKEN full-length enriched library, clone:1110007P09 product:solute carrier family 25 (mitochondrial carnitine/acylcarnitine translocase), member 20, full insert sequence.
BC029733 - Mus musculus solute carrier family 25 (mitochondrial carnitine/acylcarnitine translocase), member 20, mRNA (cDNA clone MGC:25786 IMAGE:4019406), complete cds.
AK152436 - Mus musculus bone marrow macrophage cDNA, RIKEN full-length enriched library, clone:I830072E12 product:solute carrier family 25 (mitochondrial carnitine/acylcarnitine translocase), member 20, full insert sequence.
AB017112 - Mus musculus mCAC gene, complete cds.
BC052871 - Mus musculus solute carrier family 25 (mitochondrial carnitine/acylcarnitine translocase), member 20, mRNA (cDNA clone MGC:60486 IMAGE:30032324), complete cds.
AK211911 - Mus musculus cDNA, clone:Y2G0123E07, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000035222, based on BLAT search.
AK183069 - Mus musculus cDNA, clone:Y0G0124K02, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000035222, based on BLAT search.
AK177588 - Mus musculus cDNA, clone:Y0G0103E22, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000035222, based on BLAT search.
AK215475 - Mus musculus cDNA, clone:Y2G0135C22, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000035222, based on BLAT search.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9Z2Z6 (Reactome details) participates in the following event(s):

R-MMU-200424 Exchange of palmitoylcarnitine and carnitine across the inner mitochondrial membrane
R-MMU-200425 Import of palmitoyl-CoA into the mitochondrial matrix
R-MMU-8978868 Fatty acid metabolism
R-MMU-556833 Metabolism of lipids
R-MMU-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: Cac, ENSMUST00000035222.1, ENSMUST00000035222.2, ENSMUST00000035222.3, ENSMUST00000035222.4, ENSMUST00000035222.5, MCAT_MOUSE, NM_020520, Q3U801, Q9Z2Z6, uc009rqs.1, uc009rqs.2
UCSC ID: ENSMUST00000035222.6
RefSeq Accession: NM_020520
Protein: Q9Z2Z6 (aka MCAT_MOUSE)

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.