Human Gene NACA (ENST00000678416.1) from GENCODE V44
  Description: The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data. (from UniProt F8VZJ2)
RefSeq Summary (NM_005594): This gene encodes a protein that associates with basic transcription factor 3 (BTF3) to form the nascent polypeptide-associated complex (NAC). This complex binds to nascent proteins that lack a signal peptide motif as they emerge from the ribosome, blocking interaction with the signal recognition particle (SRP) and preventing mistranslocation to the endoplasmic reticulum. This protein is an IgE autoantigen in atopic dermatitis patients. Alternative splicing results in multiple transcript variants, but the full length nature of some of these variants, including those encoding very large proteins, has not been determined. There are multiple pseudogenes of this gene on different chromosomes. [provided by RefSeq, Feb 2016].
Gencode Transcript: ENST00000678416.1
Gencode Gene: ENSG00000196531.14
Transcript (Including UTRs)
   Position: hg38 chr12:56,712,317-56,725,269 Size: 12,953 Total Exon Count: 7 Strand: -
Coding Region
   Position: hg38 chr12:56,712,538-56,713,680 Size: 1,143 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesMethods
Data last updated at UCSC: 2023-08-18 09:09:47

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr12:56,712,317-56,725,269)mRNA (may differ from genome)Protein (136 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold

-  Comments and Description Text from UniProtKB
DESCRIPTION: SubName: Full=Nascent polypeptide-associated complex subunit alpha;
CAUTION: The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.

-  Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

-  MalaCards Disease Associations
  MalaCards Gene Search: NACA
Diseases sorted by gene-association score: atopic dermatitis (6), dermatitis (6), skeletal muscle cancer (2)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 126.67 RPKM in Ovary
Total median expression: 3053.87 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -166.00475-0.349 Picture PostScript Text
3' UTR -42.60221-0.193 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002715 - Nas_poly-pep-assoc_cplx

Pfam Domains:
PF01849 - NAC domain

ModBase Predicted Comparative 3D Structure on F8VZJ2
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
Protein SequenceProtein Sequence    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Cellular Component:
GO:0005854 nascent polypeptide-associated complex

-  Descriptions from all associated GenBank mRNAs
  AK223085 - Homo sapiens mRNA for nascent-polypeptide-associated complex alpha polypeptide variant, clone: KAT03301.
AK096699 - Homo sapiens cDNA FLJ39380 fis, clone PERIC2000387, weakly similar to Mus musculus transcriptional activator alpha-NAC (Naca) gene.
AK293209 - Homo sapiens cDNA FLJ53704 complete cds, moderately similar to Nascent polypeptide-associated complex subunit alpha.
AK090650 - Homo sapiens cDNA FLJ33331 fis, clone BRACE2000489, highly similar to Nascent polypeptide-associated complex subunit alpha.
AY034001 - Homo sapiens NAC alpha mRNA, complete cds.
BC106041 - Homo sapiens nascent polypeptide-associated complex alpha subunit, mRNA (cDNA clone IMAGE:2986158), complete cds.
AF054187 - Homo sapiens alpha NAC mRNA, complete cds.
X80909 - H.sapiens alpha NAC mRNA.
AY911673 - Homo sapiens nascent polypeptide-associated complex alpha subunit (NACA) mRNA, complete cds.
BC105120 - Homo sapiens nascent polypeptide-associated complex alpha subunit, mRNA (cDNA clone IMAGE:8144123), complete cds.
BC105122 - Homo sapiens nascent polypeptide-associated complex alpha subunit, mRNA (cDNA clone IMAGE:8144125), complete cds.
AK311904 - Homo sapiens cDNA, FLJ92170, Homo sapiens nascent-polypeptide-associated complex alpha polypeptide (NACA), mRNA.
AB463919 - Synthetic construct DNA, clone: pF1KB8216, Homo sapiens NACA gene for nascent polypeptide-associated complex alpha subunit, without stop codon, in Flexi system.
CR450295 - Homo sapiens full open reading frame cDNA clone RZPDo834C011D for gene NACA, nascent-polypeptide-associated complex alpha polypeptide; complete cds; without stopcodon.
AY605660 - Homo sapiens HSD48 mRNA, partial cds.
JD020595 - Sequence 1619 from Patent EP1572962.
JD020596 - Sequence 1620 from Patent EP1572962.
JD030251 - Sequence 11275 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: AF054187, F8VZJ2, F8VZJ2_HUMAN, uc288tdx.1
UCSC ID: ENST00000678416.1
RefSeq Accession: NM_005594
Protein: F8VZJ2

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.