Human Gene ENSG00000285625 (ENST00000647707.1) from GENCODE V44
  Description: ENSG00000285625 (from geneSymbol)
RefSeq Summary (NM_000946): The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit. [provided by RefSeq, Jul 2008].
Gencode Transcript: ENST00000647707.1
Gencode Gene: ENSG00000285625.1
Transcript (Including UTRs)
   Position: hg38 chr12:56,714,612-56,741,535 Size: 26,924 Total Exon Count: 7 Strand: -
Coding Region
   Position: hg38 chr12:56,714,612-56,741,535 Size: 26,924 Coding Exon Count: 7 

Page IndexSequence and LinksPrimersMicroarray ExpressionOther SpeciesmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 09:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr12:56,714,612-56,741,535)mRNA (may differ from genome)Protein (191 aa)
Gene SorterGenome BrowserOther Species FASTATable SchemaAlphaFoldBioGPS
EnsemblExonPrimerGencodeGeneCardsMGIPubMed
UniProtKB

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
      
      
      
      
      

-  Descriptions from all associated GenBank mRNAs
  AK223085 - Homo sapiens mRNA for nascent-polypeptide-associated complex alpha polypeptide variant, clone: KAT03301.
AK096699 - Homo sapiens cDNA FLJ39380 fis, clone PERIC2000387, weakly similar to Mus musculus transcriptional activator alpha-NAC (Naca) gene.
AK293209 - Homo sapiens cDNA FLJ53704 complete cds, moderately similar to Nascent polypeptide-associated complex subunit alpha.
AK090650 - Homo sapiens cDNA FLJ33331 fis, clone BRACE2000489, highly similar to Nascent polypeptide-associated complex subunit alpha.
AY034001 - Homo sapiens NAC alpha mRNA, complete cds.
BC106041 - Homo sapiens nascent polypeptide-associated complex alpha subunit, mRNA (cDNA clone IMAGE:2986158), complete cds.
AF054187 - Homo sapiens alpha NAC mRNA, complete cds.
X80909 - H.sapiens alpha NAC mRNA.
AY911673 - Homo sapiens nascent polypeptide-associated complex alpha subunit (NACA) mRNA, complete cds.
BC105120 - Homo sapiens nascent polypeptide-associated complex alpha subunit, mRNA (cDNA clone IMAGE:8144123), complete cds.
BC105122 - Homo sapiens nascent polypeptide-associated complex alpha subunit, mRNA (cDNA clone IMAGE:8144125), complete cds.
AK311904 - Homo sapiens cDNA, FLJ92170, Homo sapiens nascent-polypeptide-associated complex alpha polypeptide (NACA), mRNA.
AB463919 - Synthetic construct DNA, clone: pF1KB8216, Homo sapiens NACA gene for nascent polypeptide-associated complex alpha subunit, without stop codon, in Flexi system.
CR450295 - Homo sapiens full open reading frame cDNA clone RZPDo834C011D for gene NACA, nascent-polypeptide-associated complex alpha polypeptide; complete cds; without stopcodon.
AY605660 - Homo sapiens HSD48 mRNA, partial cds.
AK223391 - Homo sapiens mRNA for DNA primase small subunit, 49kDa variant, clone: FCC107C02.
X74330 - H.sapiens mRNA for DNA primase (subunit p48).
BC005266 - Homo sapiens primase, DNA, polypeptide 1 (49kDa), mRNA (cDNA clone MGC:12308 IMAGE:3686937), complete cds.
AK314866 - Homo sapiens cDNA, FLJ95767, highly similar to Homo sapiens primase, polypeptide 1, 49kDa (PRIM1), mRNA.
KJ891881 - Synthetic construct Homo sapiens clone ccsbBroadEn_01275 PRIM1 gene, encodes complete protein.
EU176421 - Synthetic construct Homo sapiens clone IMAGE:100006499; FLH193115.01X; RZPDo839D06252D primase, DNA, polypeptide 1 (49kDa) (PRIM1) gene, encodes complete protein.
EU176762 - Synthetic construct Homo sapiens clone IMAGE:100011533; FLH193111.01L; RZPDo839A02256D primase, DNA, polypeptide 1 (49kDa) (PRIM1) gene, encodes complete protein.
AB528338 - Synthetic construct DNA, clone: pF1KB0958, Homo sapiens PRIM1 gene for primase, DNA, polypeptide 1, without stop codon, in Flexi system.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa00230 - Purine metabolism
hsa00240 - Pyrimidine metabolism
hsa01100 - Metabolic pathways
hsa03030 - DNA replication

-  Other Names for This Gene
  Alternate Gene Symbols: A0A3B3ITS8, AB528338, uc285kgz.1
UCSC ID: ENST00000647707.1
RefSeq Accession: NM_000946

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.