Human Gene NACA (ENST00000546392.6) from GENCODE V39
Description: Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites). May act as a specific coactivator for JUN, binding to DNA and stabilizing the interaction of JUN homodimers with target gene promoters. (from UniProt Q13765) RefSeq Summary (NM_001320193): This gene encodes a protein that associates with basic transcription factor 3 (BTF3) to form the nascent polypeptide-associated complex (NAC). This complex binds to nascent proteins that lack a signal peptide motif as they emerge from the ribosome, blocking interaction with the signal recognition particle (SRP) and preventing mistranslocation to the endoplasmic reticulum. This protein is an IgE autoantigen in atopic dermatitis patients. Alternative splicing results in multiple transcript variants, but the full length nature of some of these variants, including those encoding very large proteins, has not been determined. There are multiple pseudogenes of this gene on different chromosomes. [provided by RefSeq, Feb 2016]. Gencode Transcript: ENST00000546392.6 Gencode Gene: ENSG00000196531.14 Transcript (Including UTRs) Position: hg38 chr12:56,712,504-56,725,516 Size: 13,013 Total Exon Count: 8 Strand: - Coding Region Position: hg38 chr12:56,712,538-56,724,521 Size: 11,984 Coding Exon Count: 7
ID:NACA_HUMAN DESCRIPTION: RecName: Full=Nascent polypeptide-associated complex subunit alpha; Short=NAC-alpha; AltName: Full=Alpha-NAC; AltName: Allergen=Hom s 2; FUNCTION: Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites). May act as a specific coactivator for JUN, binding to DNA and stabilizing the interaction of JUN homodimers with target gene promoters. SUBUNIT: Interacts with TBP and JUN (By similarity). Part of the nascent polypeptide-associated complex (NAC), consisting of NACA and BTF3. NAC associates with ribosomes through the BTF3 subunit. Both subunits can contact nascent polypeptide chains. Interacts with ASFV protein H339R. INTERACTION: P40222:TXLNA; NbExp=2; IntAct=EBI-712216, EBI-359793; SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. Also found in nucleus. TISSUE SPECIFICITY: Ubiquitously expressed. PTM: Phosphorylation of Ser-43 by ILK during cell adhesion may promote nuclear localization. Phosphorylation of Thr-159 by GSK3B may promote proteasome mediated degradation (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR. ALLERGEN: Causes an allergic reaction in human. Binds to IgE from atopic dermatitis (AD) patients. Identified as an IgE autoantigen in atopic dermatitis (AD) patients with severe skin manifestations. SIMILARITY: Belongs to the NAC-alpha family. SIMILARITY: Contains 1 NAC-A/B (NAC-alpha/beta) domain. SIMILARITY: Contains 1 UBA domain. SEQUENCE CAUTION: Sequence=AAV83778.1; Type=Erroneous initiation;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q13765
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0003231 cardiac ventricle development GO:0006351 transcription, DNA-templated GO:0006412 translation GO:0010664 negative regulation of striated muscle cell apoptotic process GO:0015031 protein transport GO:0016032 viral process GO:0043403 skeletal muscle tissue regeneration GO:0048633 positive regulation of skeletal muscle tissue growth GO:0048742 regulation of skeletal muscle fiber development GO:0061384 heart trabecula morphogenesis GO:1901227 negative regulation of transcription from RNA polymerase II promoter involved in heart development GO:1901228 positive regulation of transcription from RNA polymerase II promoter involved in heart development GO:2000138 positive regulation of cell proliferation involved in heart morphogenesis