Mouse Gene Adad1 (ENSMUST00000144629.7) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus adenosine deaminase domain containing 1 (testis specific) (Adad1), mRNA. (from RefSeq NM_009350)
Gencode Transcript: ENSMUST00000144629.7
Gencode Gene: ENSMUSG00000027719.15
Transcript (Including UTRs)
   Position: mm10 chr3:37,063,527-37,112,512 Size: 48,986 Total Exon Count: 12 Strand: +
Coding Region
   Position: mm10 chr3:37,063,723-37,111,467 Size: 47,745 Coding Exon Count: 12 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-20

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr3:37,063,527-37,112,512)mRNA (may differ from genome)Protein (619 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Adenosine deaminase domain-containing protein 1; AltName: Full=Testis nuclear RNA-binding protein;
FUNCTION: Plays a role in spermatogenesis. Binds to RNA but not to DNA.
TISSUE SPECIFICITY: Testis-specific. Detected in round spermatid cells from stage II-XI (at protein level). Expressed in germ cells from mid-pachytene spermatocytes to mid-round spermatids.
DISRUPTION PHENOTYPE: Mice exhibit spermatozoa retention in stage IX tubules and a reduction in the number of sperm in the epididymis, leading to a reduction in fertility.
SIMILARITY: Belongs to the ADAD family.
SIMILARITY: Contains 1 A to I editase domain.
SIMILARITY: Contains 1 DRBM (double-stranded RNA-binding) domain.
SEQUENCE CAUTION: Sequence=CAM18414.1; Type=Erroneous gene model prediction; Sequence=CAM28072.1; Type=Erroneous gene model prediction; Sequence=CAM28078.1; Type=Erroneous gene model prediction;

-  Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -86.30196-0.440 Picture PostScript Text
3' UTR -206.901045-0.198 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002466 - A_deamin
IPR001159 - Ds-RNA-bd
IPR014720 - dsRNA-bd-like

Pfam Domains:
PF00035 - Double-stranded RNA binding motif
PF02137 - Adenosine-deaminase (editase) domain

SCOP Domains:
54768 - dsRNA-binding domain-like

ModBase Predicted Comparative 3D Structure on Q5SUE7
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
Protein SequenceProtein Sequence    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003723 RNA binding
GO:0004000 adenosine deaminase activity

Biological Process:
GO:0006396 RNA processing
GO:0007275 multicellular organism development
GO:0007283 spermatogenesis
GO:0007286 spermatid development
GO:0030154 cell differentiation

Cellular Component:
GO:0005634 nucleus

-  Descriptions from all associated GenBank mRNAs
  X84693 - M.musculus Tenr mRNA for RNA binding protein.
BC165984 - Synthetic construct Mus musculus clone IMAGE:100066284, MGC:195421 adenosine deaminase domain containing 1 (testis specific) (Adad1) mRNA, encodes complete protein.
AK133008 - Mus musculus adult male testis cDNA, RIKEN full-length enriched library, clone:4930551A18 product:testis nuclear RNA binding protein, full insert sequence.
BC107346 - Mus musculus adenosine deaminase domain containing 1 (testis specific), mRNA (cDNA clone IMAGE:40055356), complete cds.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
PWY-6353 - purine nucleotides degradation II (aerobic)
PWY-6609 - adenine and adenosine salvage III
PWY0-1296 - purine ribonucleosides degradation to ribose-1-phosphate
SALVADEHYPOX-PWY - adenosine nucleotides degradation II

-  Other Names for This Gene
  Alternate Gene Symbols: A2AAD7, A2AAD8, A2AAV1, ADAD1_MOUSE, NM_009350, Q08EL2, Q3V0N7, Q5SUE7, Q62309, Q9JLB6, Tenr, uc008pah.1, uc008pah.2
UCSC ID: uc008pah.2
RefSeq Accession: NM_009350
Protein: Q5SUE7 (aka ADAD1_MOUSE)
CCDS: CCDS17315.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.