Mouse Gene Lefty2 (ENSMUST00000085797.5) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus left-right determination factor 2 (Lefty2), mRNA. (from RefSeq NM_177099)
RefSeq Summary (NM_177099): This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in left-right asymmetry determination of organ systems during development. This protein is also important in self-renewal and differentiation of mouse embryonic stem cells. Mice lacking a functional copy of this gene exhibit defects in axial and left-right patterning. [provided by RefSeq, Aug 2016].
Gencode Transcript: ENSMUST00000085797.5
Gencode Gene: ENSMUSG00000066652.5
Transcript (Including UTRs)
   Position: mm10 chr1:180,893,108-180,899,103 Size: 5,996 Total Exon Count: 4 Strand: +
Coding Region
   Position: mm10 chr1:180,893,184-180,897,813 Size: 4,630 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-20

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr1:180,893,108-180,899,103)mRNA (may differ from genome)Protein (368 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Left-right determination factor 2; AltName: Full=Left-right determination factor B; AltName: Full=Protein lefty-2; AltName: Full=Protein lefty-B; Flags: Precursor;
FUNCTION: Required for left-right asymmetry determination of organ systems in mammals.
DEVELOPMENTAL STAGE: At the primitive streak stage (E7.0), expressed in the emerging mesoderm. By E8.0, expressed exclusively on the left side of developing embryos with expression predominantly in the lateral-plate mesoderm (LPM). Weak expression in the prospective floor plate (PFP).
PTM: The processing of the protein may also occur at the second R- X-X-R site located at AA 132-135. Processing appears to be regulated in a cell-type specific manner.
SIMILARITY: Belongs to the TGF-beta family.

-  Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -14.7076-0.193 Picture PostScript Text
3' UTR -351.801290-0.273 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR003942 - LRDF
IPR001839 - TGF-b_C
IPR001111 - TGF-b_N
IPR015615 - TGF-beta-rel
IPR017948 - TGFb_CS

Pfam Domains:
PF00019 - Transforming growth factor beta like domain
PF00688 - TGF-beta propeptide

SCOP Domains:
57501 - Cystine-knot cytokines

ModBase Predicted Comparative 3D Structure on P57785
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005125 cytokine activity
GO:0005160 transforming growth factor beta receptor binding
GO:0008083 growth factor activity

Biological Process:
GO:0007275 multicellular organism development
GO:0010469 regulation of receptor activity
GO:0010862 positive regulation of pathway-restricted SMAD protein phosphorylation
GO:0030509 BMP signaling pathway
GO:0042981 regulation of apoptotic process
GO:0043408 regulation of MAPK cascade
GO:0048468 cell development
GO:0060395 SMAD protein signal transduction

Cellular Component:
GO:0005576 extracellular region
GO:0005615 extracellular space

-  Descriptions from all associated GenBank mRNAs
  LF193053 - JP 2014500723-A/556: Polycomb-Associated Non-Coding RNAs.
AK031621 - Mus musculus 13 days embryo male testis cDNA, RIKEN full-length enriched library, clone:6030463A22 product:LEFT-RIGHT DETERMINATION FACTOR B PRECURSOR (LEFTY-2 PROTEIN), full insert sequence.
AK131960 - Mus musculus ES cells cDNA, RIKEN full-length enriched library, clone:2410142I20 product:endometrial bleeding associated factor, full insert sequence.
BC066224 - Mus musculus left-right determination factor 2, mRNA (cDNA clone MGC:76786 IMAGE:30008028), complete cds.
LF220402 - JP 2014500723-A/27905: Polycomb-Associated Non-Coding RNAs.
LF220404 - JP 2014500723-A/27907: Polycomb-Associated Non-Coding RNAs.
LF220408 - JP 2014500723-A/27911: Polycomb-Associated Non-Coding RNAs.
LF220451 - JP 2014500723-A/27954: Polycomb-Associated Non-Coding RNAs.
LF220411 - JP 2014500723-A/27914: Polycomb-Associated Non-Coding RNAs.
LF220454 - JP 2014500723-A/27957: Polycomb-Associated Non-Coding RNAs.
LF220413 - JP 2014500723-A/27916: Polycomb-Associated Non-Coding RNAs.
LF220420 - JP 2014500723-A/27923: Polycomb-Associated Non-Coding RNAs.
LF220422 - JP 2014500723-A/27925: Polycomb-Associated Non-Coding RNAs.
LF220424 - JP 2014500723-A/27927: Polycomb-Associated Non-Coding RNAs.
LF220426 - JP 2014500723-A/27929: Polycomb-Associated Non-Coding RNAs.
LF220427 - JP 2014500723-A/27930: Polycomb-Associated Non-Coding RNAs.
LF220429 - JP 2014500723-A/27932: Polycomb-Associated Non-Coding RNAs.
LF220430 - JP 2014500723-A/27933: Polycomb-Associated Non-Coding RNAs.
LF233408 - JP 2014500723-A/40911: Polycomb-Associated Non-Coding RNAs.
LF220431 - JP 2014500723-A/27934: Polycomb-Associated Non-Coding RNAs.
LF220433 - JP 2014500723-A/27936: Polycomb-Associated Non-Coding RNAs.
LF220386 - JP 2014500723-A/27889: Polycomb-Associated Non-Coding RNAs.
LF220435 - JP 2014500723-A/27938: Polycomb-Associated Non-Coding RNAs.
MA428630 - JP 2018138019-A/556: Polycomb-Associated Non-Coding RNAs.
MA455979 - JP 2018138019-A/27905: Polycomb-Associated Non-Coding RNAs.
MA455981 - JP 2018138019-A/27907: Polycomb-Associated Non-Coding RNAs.
MA455985 - JP 2018138019-A/27911: Polycomb-Associated Non-Coding RNAs.
MA456028 - JP 2018138019-A/27954: Polycomb-Associated Non-Coding RNAs.
MA455988 - JP 2018138019-A/27914: Polycomb-Associated Non-Coding RNAs.
MA456031 - JP 2018138019-A/27957: Polycomb-Associated Non-Coding RNAs.
MA455990 - JP 2018138019-A/27916: Polycomb-Associated Non-Coding RNAs.
MA455997 - JP 2018138019-A/27923: Polycomb-Associated Non-Coding RNAs.
MA455999 - JP 2018138019-A/27925: Polycomb-Associated Non-Coding RNAs.
MA456001 - JP 2018138019-A/27927: Polycomb-Associated Non-Coding RNAs.
MA456003 - JP 2018138019-A/27929: Polycomb-Associated Non-Coding RNAs.
MA456004 - JP 2018138019-A/27930: Polycomb-Associated Non-Coding RNAs.
MA456006 - JP 2018138019-A/27932: Polycomb-Associated Non-Coding RNAs.
MA456007 - JP 2018138019-A/27933: Polycomb-Associated Non-Coding RNAs.
MA456008 - JP 2018138019-A/27934: Polycomb-Associated Non-Coding RNAs.
MA456010 - JP 2018138019-A/27936: Polycomb-Associated Non-Coding RNAs.
MA455963 - JP 2018138019-A/27889: Polycomb-Associated Non-Coding RNAs.
MA456012 - JP 2018138019-A/27938: Polycomb-Associated Non-Coding RNAs.
MA468985 - JP 2018138019-A/40911: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
mmu04350 - TGF-beta signaling pathway

Reactome (by CSHL, EBI, and GO)

Protein P57785 (Reactome details) participates in the following event(s):

R-MMU-1181347 Lefty binds Nodal
R-MMU-1181357 Lefty binds EGF-CFC coreceptor
R-MMU-481007 Exocytosis of platelet alpha granule contents
R-MMU-114608 Platelet degranulation
R-MMU-76005 Response to elevated platelet cytosolic Ca2+
R-MMU-76002 Platelet activation, signaling and aggregation
R-MMU-109582 Hemostasis

-  Other Names for This Gene
  Alternate Gene Symbols: Leftb, LFTY2_MOUSE, NM_177099, P57785, Q8BMF7, uc007dwu.1, uc007dwu.2, uc007dwu.3
UCSC ID: uc007dwu.3
RefSeq Accession: NM_177099
Protein: P57785 (aka LFTY2_MOUSE)
CCDS: CCDS15575.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.