Mouse Gene Wnt3 (ENSMUST00000000127.5) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus wingless-type MMTV integration site family, member 3 (Wnt3), mRNA. (from RefSeq NM_009521)
Gencode Transcript: ENSMUST00000000127.5
Gencode Gene: ENSMUSG00000000125.5
Transcript (Including UTRs)
   Position: mm10 chr11:103,774,150-103,817,957 Size: 43,808 Total Exon Count: 5 Strand: +
Coding Region
   Position: mm10 chr11:103,774,269-103,812,760 Size: 38,492 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-20

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr11:103,774,150-103,817,957)mRNA (may differ from genome)Protein (355 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Proto-oncogene Wnt-3; AltName: Full=Proto-oncogene Int-4; Flags: Precursor;
FUNCTION: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
SUBUNIT: Interacts with PORCN. Interacts with WLS.
INTERACTION: O35082:Kl; NbExp=4; IntAct=EBI-1570853, EBI-1570828;
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.
TISSUE SPECIFICITY: Dorsal portion of the neural tube, dorsal ectoderm, the branchial arches, and the limb buds.
PTM: Palmitoylation at Ser-212 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-80. Palmitoylation is necessary for proper trafficking to cell surface (By similarity).
DISEASE: Note=Some mouse mammary tumors induced by mouse mammary tumor virus (MMTV) contain a provirus integrated into a host cell region which has been named Wnt3.
SIMILARITY: Belongs to the Wnt family.

-  Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -25.70119-0.216 Picture PostScript Text
3' UTR -600.001889-0.318 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR005817 - Wnt
IPR009141 - Wnt3
IPR018161 - Wnt_CS

Pfam Domains:
PF00110 - wnt family

ModBase Predicted Comparative 3D Structure on P17553
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologNo ortholog
 Protein Sequence    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005102 receptor binding
GO:0005109 frizzled binding
GO:0005515 protein binding
GO:0019904 protein domain specific binding
GO:0048018 receptor agonist activity

Biological Process:
GO:0000902 cell morphogenesis
GO:0001707 mesoderm formation
GO:0007165 signal transduction
GO:0007267 cell-cell signaling
GO:0007275 multicellular organism development
GO:0007276 gamete generation
GO:0007411 axon guidance
GO:0009887 animal organ morphogenesis
GO:0009948 anterior/posterior axis specification
GO:0009950 dorsal/ventral axis specification
GO:0009952 anterior/posterior pattern specification
GO:0010469 regulation of receptor activity
GO:0010628 positive regulation of gene expression
GO:0016055 Wnt signaling pathway
GO:0030177 positive regulation of Wnt signaling pathway
GO:0035115 embryonic forelimb morphogenesis
GO:0035116 embryonic hindlimb morphogenesis
GO:0044338 canonical Wnt signaling pathway involved in mesenchymal stem cell differentiation
GO:0044339 canonical Wnt signaling pathway involved in osteoblast differentiation
GO:0045165 cell fate commitment
GO:0048646 anatomical structure formation involved in morphogenesis
GO:0048697 positive regulation of collateral sprouting in absence of injury
GO:0048843 negative regulation of axon extension involved in axon guidance
GO:0050767 regulation of neurogenesis
GO:0060064 Spemann organizer formation at the anterior end of the primitive streak
GO:0060070 canonical Wnt signaling pathway
GO:0060173 limb development
GO:0060174 limb bud formation
GO:0060323 head morphogenesis
GO:0061180 mammary gland epithelium development
GO:0072089 stem cell proliferation
GO:1904954 canonical Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation
GO:1905474 canonical Wnt signaling pathway involved in stem cell proliferation

Cellular Component:
GO:0005576 extracellular region
GO:0005615 extracellular space
GO:0005737 cytoplasm
GO:0005788 endoplasmic reticulum lumen

-  Descriptions from all associated GenBank mRNAs
  AK132516 - Mus musculus 10 days neonate skin cDNA, RIKEN full-length enriched library, clone:4732488C12 product:wingless-related MMTV integration site 3, full insert sequence.
LF204694 - JP 2014500723-A/12197: Polycomb-Associated Non-Coding RNAs.
M32502 - Mouse proto-oncogene Wnt-3 protein mRNA, complete cds.
BC152305 - Mus musculus wingless-related MMTV integration site 3, mRNA (cDNA clone MGC:129153 IMAGE:40039312), complete cds.
BC152537 - Mus musculus wingless-related MMTV integration site 3, mRNA (cDNA clone MGC:173137 IMAGE:40040396), complete cds.
BC152304 - Mus musculus wingless-related MMTV integration site 3, mRNA (cDNA clone MGC:129152 IMAGE:40039305), complete cds.
AK141154 - Mus musculus 0 day neonate cerebellum cDNA, RIKEN full-length enriched library, clone:C230075J09 product:wingless-related MMTV integration site 3, full insert sequence.
BC141271 - Mus musculus wingless-related MMTV integration site 3, mRNA (cDNA clone MGC:176232 IMAGE:9055883), complete cds.
BC141274 - Mus musculus wingless-related MMTV integration site 3, mRNA (cDNA clone MGC:176235 IMAGE:9055886), complete cds.
AK043014 - Mus musculus 7 days neonate cerebellum cDNA, RIKEN full-length enriched library, clone:A730047N19 product:WNT-3 PROTO-ONCOGENE PROTEIN PRECURSOR homolog [Mus musculus], full insert sequence.
AK163878 - Mus musculus 0 day neonate cerebellum cDNA, RIKEN full-length enriched library, clone:C230004J11 product:wingless-related MMTV integration site 3, full insert sequence.
MA440271 - JP 2018138019-A/12197: Polycomb-Associated Non-Coding RNAs.
AK161781 - Mus musculus 13 days embryo forelimb cDNA, RIKEN full-length enriched library, clone:5930403J03 product:wingless-related MMTV integration site 3, full insert sequence.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
mmu04310 - Wnt signaling pathway
mmu04340 - Hedgehog signaling pathway
mmu04916 - Melanogenesis
mmu05200 - Pathways in cancer
mmu05217 - Basal cell carcinoma

Reactome (by CSHL, EBI, and GO)

Protein P17553 (Reactome details) participates in the following event(s):

R-MMU-3247840 WLS binds WNT ligands in the Golgi
R-MMU-3247843 secretion of WNT ligands
R-MMU-3238698 WNT ligand biogenesis and trafficking
R-MMU-195721 Signaling by WNT
R-MMU-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: Int-4, NM_009521, P17553, uc007lvs.1, uc007lvs.2, uc007lvs.3, Wnt-3, WNT3_MOUSE
UCSC ID: uc007lvs.3
RefSeq Accession: NM_009521
Protein: P17553 (aka WNT3_MOUSE)
CCDS: CCDS25523.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.