Description: Homo sapiens integrin-linked kinase (ILK), transcript variant 1, mRNA. RefSeq Summary (NM_004517): This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]. Transcript (Including UTRs) Position: hg19 chr11:6,624,964-6,632,099 Size: 7,136 Total Exon Count: 13 Strand: + Coding Region Position: hg19 chr11:6,625,502-6,631,842 Size: 6,341 Coding Exon Count: 12
ID:ILK_HUMAN DESCRIPTION: RecName: Full=Integrin-linked protein kinase; EC=2.7.11.1; AltName: Full=59 kDa serine/threonine-protein kinase; AltName: Full=ILK-1; AltName: Full=ILK-2; AltName: Full=p59ILK; FUNCTION: Receptor-proximal protein kinase regulating integrin- mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. ENZYME REGULATION: Stimulated rapidly but transiently by both cell fibronectin interactions, as well as by insulin, in a PI3-K- dependent manner, likely via the binding of PtdIns(3,4,5)P3 with a PH-like domain of ILK. SUBUNIT: Interacts with cytoplasmic domain of beta 1 subunit of integrin. Could also interacts with beta 2, beta 3 and/or beta 5 subunit of integrin. Interacts (via ANK repeats) with LIMS1 and LIMS2. Interacts with parvins and probably TGFB1I1. Interacts (via ANK repeats) with EPHA1 (via SAM domain); stimulated by EFNA1 but independent of the kinase activity of EPHA1. INTERACTION: Q14790:CASP8; NbExp=2; IntAct=EBI-747644, EBI-78060; P55211:CASP9; NbExp=2; IntAct=EBI-747644, EBI-516799; Q9H0C8:ILKAP; NbExp=3; IntAct=EBI-747644, EBI-2620298; Q9NVD7:PARVA; NbExp=2; IntAct=EBI-747644, EBI-747655; Q6R327:RICTOR; NbExp=8; IntAct=EBI-747644, EBI-1387196; Q9BWU0:SLC4A1AP; NbExp=8; IntAct=EBI-747644, EBI-1999704; Q9UMS6:SYNPO2; NbExp=6; IntAct=EBI-747644, EBI-3453434; SUBCELLULAR LOCATION: Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. TISSUE SPECIFICITY: Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver. DOMAIN: A PH-like domain is involved in phosphatidylinositol phosphate binding. PTM: Autophosphorylated on serine residues. SIMILARITY: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. SIMILARITY: Contains 5 ANK repeats. SIMILARITY: Contains 1 protein kinase domain. SEQUENCE CAUTION: Sequence=CAB94832.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact. Was originally thought to be distinct gene (ILK2) (PubMed:10871859);
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q13418
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
LF207829 - JP 2014500723-A/15332: Polycomb-Associated Non-Coding RNAs. LF207831 - JP 2014500723-A/15334: Polycomb-Associated Non-Coding RNAs. AK293474 - Homo sapiens cDNA FLJ53825 complete cds, highly similar to Integrin-linked protein kinase 1 (EC 2.7.11.1). AK314843 - Homo sapiens cDNA, FLJ95734. U40282 - Homo sapiens integrin-linked kinase (ILK) mRNA, complete cds. AK296628 - Homo sapiens cDNA FLJ50979 complete cds, moderately similar to Integrin-linked protein kinase (EC 2.7.11.1). BC001554 - Homo sapiens integrin-linked kinase, mRNA (cDNA clone MGC:5051 IMAGE:3457801), complete cds. AB209416 - Homo sapiens mRNA for integrin-linked kinase variant protein. CR749220 - Homo sapiens mRNA; cDNA DKFZp686F1765 (from clone DKFZp686F1765). EU794625 - Homo sapiens epididymis secretory protein Li 28 (HEL-S-28) mRNA, complete cds. JD038744 - Sequence 19768 from Patent EP1572962. JD555098 - Sequence 536122 from Patent EP1572962. KJ897056 - Synthetic construct Homo sapiens clone ccsbBroadEn_06450 ILK gene, encodes complete protein. KJ905216 - Synthetic construct Homo sapiens clone ccsbBroadEn_14673 ILK gene, encodes complete protein. KR709573 - Synthetic construct Homo sapiens clone CCSBHm_00003606 ILK (ILK) mRNA, encodes complete protein. CU674762 - Synthetic construct Homo sapiens gateway clone IMAGE:100019468 5' read ILK mRNA. AB463785 - Synthetic construct DNA, clone: pF1KB8187, Homo sapiens ILK gene for integrin-linked kinase, without stop codon, in Flexi system. CR407673 - Homo sapiens full open reading frame cDNA clone RZPDo834B113D for gene ILK, integrin-linked kinase complete cds, without stopcodon. DQ891172 - Synthetic construct clone IMAGE:100003802; FLH178834.01X; RZPDo839H08128D integrin-linked kinase (ILK) gene, encodes complete protein. DQ894355 - Synthetic construct Homo sapiens clone IMAGE:100008815; FLH178830.01L; RZPDo839H08127D integrin-linked kinase (ILK) gene, encodes complete protein. LF369387 - JP 2014500723-A/176890: Polycomb-Associated Non-Coding RNAs. JD158576 - Sequence 139600 from Patent EP1572962. LF369392 - JP 2014500723-A/176895: Polycomb-Associated Non-Coding RNAs. JD521671 - Sequence 502695 from Patent EP1572962. JD060336 - Sequence 41360 from Patent EP1572962. JD293820 - Sequence 274844 from Patent EP1572962. JD527351 - Sequence 508375 from Patent EP1572962. JD518710 - Sequence 499734 from Patent EP1572962. JD384971 - Sequence 365995 from Patent EP1572962. LF369393 - JP 2014500723-A/176896: Polycomb-Associated Non-Coding RNAs. JD445947 - Sequence 426971 from Patent EP1572962. MA604964 - JP 2018138019-A/176890: Polycomb-Associated Non-Coding RNAs. MA604969 - JP 2018138019-A/176895: Polycomb-Associated Non-Coding RNAs. MA604970 - JP 2018138019-A/176896: Polycomb-Associated Non-Coding RNAs. MA443406 - JP 2018138019-A/15332: Polycomb-Associated Non-Coding RNAs. MA443408 - JP 2018138019-A/15334: Polycomb-Associated Non-Coding RNAs.
Biochemical and Signaling Pathways
KEGG - Kyoto Encyclopedia of Genes and Genomes hsa03320 - PPAR signaling pathway hsa04510 - Focal adhesion hsa05100 - Bacterial invasion of epithelial cells hsa05213 - Endometrial cancer
BioCarta from NCI Cancer Genome Anatomy Project h_ptenPathway - PTEN dependent cell cycle arrest and apoptosis
Reactome (by CSHL, EBI, and GO)
Protein Q13418 (Reactome details) participates in the following event(s):
R-HSA-432897 ILK interacts with beta-1 integrin R-HSA-446391 Formation of the PINCH-ILK-parvin complex R-HSA-446343 Localization of the PINCH-ILK-PARVIN complex to focal adhesions R-HSA-446353 Cell-extracellular matrix interactions R-HSA-446728 Cell junction organization R-HSA-1500931 Cell-Cell communication