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CFTR — PRKAA2
Text-mined interactions from Literome
Hallows et al., J Clin Invest 2000
:
Because AMPK is a metabolic sensor in cells and responds to changes in cellular ATP,
regulation of
CFTR by
AMPK may be important in inhibiting CFTR under conditions of metabolic stress, thereby linking transepithelial transport to cell metabolic state
Hallows et al., J Biol Chem 2003
(Ion Channel Gating) :
Plasma membrane expression of
CFTR , assessed by surface biotinylation, was not
affected by
AMPK activation
King et al., Am J Physiol Cell Physiol 2009
:
To further characterize the mechanism of
AMPK dependent regulation of
CFTR , whole cell patch-clamp measurements were performed with PKA activation in Calu-3 cells expressing either constitutively active or dominant negative AMPK mutants ( AMPK-CA or AMPK-DN ) ... Baseline CFTR conductance in cells expressing AMPK-DN was substantially greater than controls, suggesting that tonic
AMPK activity in these cells
inhibits CFTR under basal conditions ... Phosphorylation studies of CFTR in human embryonic kidney-293 cells using tetracycline-inducible expression of AMPK-DN demonstrated
AMPK dependent phosphorylation of
CFTR in vivo ... Thus,
AMPK dependent
CFTR phosphorylation renders the channel resistant to activation by PKA and PKC without preventing phosphorylation by these kinases ... Ser-to-Ala mutation at this site enhanced baseline CFTR activity and rendered CFTR resistant to inhibition by AMPK, suggesting that
AMPK phosphorylation at Ser768 is
required for its inhibition of
CFTR ... In summary, our findings indicate that
AMPK dependent phosphorylation of
CFTR inhibits CFTR activation by PKA, thereby tuning the PKA-responsiveness of CFTR to metabolic and other stresses in the cell
Murphy et al., Am J Physiol Cell Physiol 2009
:
Finally, inhibition of the cystic fibrosis transmembrane regulator ( CFTR ) Cl ( - ) conductance blocked depolarization of GI neurons to decreased glucose or AMPK activation, whereas decreased glucose,
AMPK activation, and 8-Br-cGMP
increased VMH
CFTR phosphorylation
King et al., J Biol Chem 2012
:
Taken together, these results suggest that NDPK-A exists in a functional cellular complex with AMPK and CFTR in airway epithelia, and NDPK-A catalytic function is required for the
AMPK dependent regulation of
CFTR