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CDC42 — PARD6A
Pathways - manually collected, often from reviews:
-
KEGG Tight junction:
CDC42
→
PARD6A/PARD6B/PARD6G
(protein-protein, activation)
-
NCI Pathway Database CDC42 signaling events:
CDC42/GTP complex (CDC42)
→
Par6/PKC zeta complex (PARD6A-PRKCZ)
(modification, activates)
Etienne-Manneville et al., Cell 2001*, Etienne-Manneville et al., Nature 2003*, Etienne-Manneville et al., J Cell Biol 2005*, Osmani et al., Curr Biol 2006*
Evidence: assay, physical interaction
-
NCI Pathway Database RhoA signaling pathway:
PKC zeta/Par6/CDC42/GTP complex (PRKCZ-PARD6A-CDC42)
→
Par3 (F2RL2)
(modification, collaborate)
Nakayama et al., Dev Cell 2008
Evidence: mutant phenotype, assay
-
NCI Pathway Database RhoA signaling pathway:
PKC zeta/Par6/CDC42/GTP complex (PRKCZ-PARD6A-CDC42)
→
PKC zeta/Par3/Par6/CDC42/GTP complex (PRKCZ-F2RL2-PARD6A-CDC42)
(modification, collaborate)
Nakayama et al., Dev Cell 2008
Evidence: mutant phenotype, assay
-
NCI Pathway Database RhoA signaling pathway:
ROCK1-ROCK2 (ROCK2/ROCK1)
→
PKC zeta/Par3/Par6/CDC42/GTP complex (PRKCZ-F2RL2-PARD6A-CDC42)
(modification, activates)
Nakayama et al., Dev Cell 2008
Evidence: mutant phenotype, assay
-
NCI Pathway Database RhoA signaling pathway:
Par3 (F2RL2)
→
PKC zeta/Par3/Par6/CDC42/GTP complex (PRKCZ-F2RL2-PARD6A-CDC42)
(modification, collaborate)
Nakayama et al., Dev Cell 2008
Evidence: mutant phenotype, assay
-
NCI Pathway Database CDC42 signaling events:
CDC42/GTP complex (CDC42)
→
PKC zeta/Par3/Par6/CDC42/GTP complex (PRKCZ-F2RL2-PARD6A-CDC42)
(modification, collaborate)
Joberty et al., Nat Cell Biol 2000, Lin et al., Nat Cell Biol 2000
Evidence: assay, physical interaction
-
NCI Pathway Database CDC42 signaling events:
CDC42/GTP complex (CDC42)
→
PKC zeta/Par3/Par6 complex (PRKCZ-F2RL2-PARD6A)
(modification, collaborate)
Joberty et al., Nat Cell Biol 2000, Lin et al., Nat Cell Biol 2000
Evidence: assay, physical interaction
-
NCI Pathway Database CDC42 signaling events:
PKC zeta/Par3/Par6/CDC42/GTP complex (PRKCZ-F2RL2-PARD6A-CDC42)
→
PKC zeta/Par3/Par6 complex (PRKCZ-F2RL2-PARD6A)
(modification, collaborate)
Joberty et al., Nat Cell Biol 2000, Lin et al., Nat Cell Biol 2000
Evidence: assay, physical interaction
-
NCI Pathway Database CDC42 signaling events:
CDC42/GTP complex (CDC42)
→
PKC epsilon/Par3/Par6/CDC42/GTP complex (PRKCE-F2RL2-PARD6A-CDC42)
(modification, collaborate)
Koh et al., J Cell Sci 2008, Koh et al., J Cell Sci 2009
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database CDC42 signaling events:
CDC42/GTP complex (CDC42)
→
PKC epsilon/Par3/Par6 complex (PRKCE-F2RL2-PARD6A)
(modification, collaborate)
Koh et al., J Cell Sci 2008, Koh et al., J Cell Sci 2009
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database CDC42 signaling events:
PKC epsilon/Par3/Par6/CDC42/GTP complex (PRKCE-F2RL2-PARD6A-CDC42)
→
PKC epsilon/Par3/Par6 complex (PRKCE-F2RL2-PARD6A)
(modification, collaborate)
Koh et al., J Cell Sci 2008, Koh et al., J Cell Sci 2009
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database CDC42 signaling events:
PKC epsilon/Par3/Par6/CDC42/GTP complex (PRKCE-F2RL2-PARD6A-CDC42)
→
PAK2-PAK4 (PAK4/PAK2)
(modification, activates)
Koh et al., J Cell Sci 2008, Koh et al., J Cell Sci 2009
Evidence: assay
-
NCI Pathway Database CDC42 signaling events:
PKC epsilon/Par3/Par6/CDC42/GTP complex (PRKCE-F2RL2-PARD6A-CDC42)
→
Src-Yes (YES1/SRC)
(modification, activates)
Koh et al., J Cell Sci 2008, Koh et al., J Cell Sci 2009
Evidence: assay
-
WikiPathways Regulation of Microtubule Cytoskeleton:
CDC42
→
PARD6A
(activation)
Protein-Protein interactions - manually collected from original source literature:
Studies that report less than 10 interactions are marked with *
-
IRef Bind Interaction:
Complex of 12 proteins
Brajenovic et al., J Biol Chem 2004
-
IRef Bind Interaction:
Complex of DMBT1-PRKCI-PRKCZ-PARD6B-CDC42-PARD6A-PARD3
Brajenovic et al., J Biol Chem 2004
-
IRef Bind_translation Interaction:
PARD6A
—
CDC42
(coimmunoprecipitation)
Brajenovic et al., J Biol Chem 2004
-
IRef Bind_translation Interaction:
PARD6A
—
CDC42
(affinity chromatography technology)
Brajenovic et al., J Biol Chem 2004
-
IRef Biogrid Interaction:
PARD6A
—
CDC42
(direct interaction, pull down)
Qiu et al., Curr Biol 2000*
-
IRef Biogrid Interaction:
PARD6A
—
CDC42
(physical association, affinity chromatography technology)
Qiu et al., Curr Biol 2000*
-
IRef Biogrid Interaction:
PARD6A
—
CDC42
(direct interaction, two hybrid)
Noda et al., Genes Cells 2001
-
IRef Biogrid Interaction:
PARD6A
—
CDC42
(direct interaction, pull down)
Noda et al., Genes Cells 2001
-
IRef Biogrid Interaction:
PARD6A
—
CDC42
(physical association, affinity chromatography technology)
Brajenovic et al., J Biol Chem 2004
-
IRef Biogrid Interaction:
PARD6A
—
CDC42
(direct interaction, two hybrid)
Joberty et al., Nat Cell Biol 2000
-
IRef Hprd Interaction:
PARD6A
—
CDC42
(in vitro)
Qiu et al., Curr Biol 2000*, Garrard et al., EMBO J 2003*
-
IRef Hprd Interaction:
PARD6A
—
CDC42
(two hybrid)
Qiu et al., Curr Biol 2000*, Garrard et al., EMBO J 2003*
-
IRef Hprd Interaction:
PARD6A
—
CDC42
(in vivo)
Qiu et al., Curr Biol 2000*, Garrard et al., EMBO J 2003*
-
IRef Intact Interaction:
Complex of 14 proteins
(association, anti tag coimmunoprecipitation)
Brajenovic et al., J Biol Chem 2004
-
IRef Intact Interaction:
PARD6A
—
CDC42
(physical association, two hybrid)
Joberty et al., Nat Cell Biol 2000
-
IRef Intact Interaction:
PARD6A
—
CDC42
(physical association, two hybrid)
Noda et al., Genes Cells 2001
-
IRef Intact Interaction:
PARD6A
—
CDC42
(physical association, pull down)
Noda et al., Genes Cells 2001
-
IRef Intact Interaction:
PARD6A
—
CDC42
(physical association, anti tag coimmunoprecipitation)
Brajenovic et al., J Biol Chem 2004
-
IRef Intact Interaction:
Complex of LLGL1-PRKCI-LLGL2-DMBT1-PRKCZ-CDC42-PARD3-PARD6A-PARD6B
(association, anti tag coimmunoprecipitation)
Brajenovic et al., J Biol Chem 2004
-
IRef Ophid Interaction:
PARD6A
—
CDC42
(aggregation, confirmational text mining)
Qiu et al., Curr Biol 2000*
-
IRef Ophid Interaction:
PARD6A
—
CDC42
(aggregation, interologs mapping)
Brown et al., Bioinformatics 2005
Text-mined interactions from Literome
Hurd et al., Nat Cell Biol 2003
:
The interaction between
Par6 and PALS1 is direct, requires the amino terminus of PALS1 and the PDZ domain of Par6, and is
regulated by
Cdc42-GTP
Garrard et al., EMBO J 2003
:
Cdc42 induces a conformational change in
Par6 , detectable by fluorescence resonance energy transfer spectroscopy
Tzima et al., J Biol Chem 2003
:
Thus, shear stimulated integrin dynamics induce polarized
Cdc42 activity, which
induces MTOC localization through the
Par6-protein kinase Czeta complex
Hutterer et al., Dev Cell 2004
:
Apical localization of
Par-6 requires its interaction with activated
Cdc42 and dominant-active or dominant negative Cdc42 disrupt epithelial polarity, suggesting that activation of this GTPase is crucial for the establishment of epithelial polarity
Liu et al., Mol Cell Biol 2004
:
Coexpression of
Par6 and ECT2 efficiently
activated Cdc42 in vivo
Gao et al., J Biol Chem 2004
:
These functional differences correlate with differences in Pals1 binding ; Par6B interacts strongly with Pals1, whereas
Par6A binds weakly to Pals1 even in the
presence of active
Cdc42
Etienne-Manneville et al., J Cell Biol 2005
:
Activation of the
Par6-PKCzeta complex by
Cdc42 at the leading edge of migrating cells promotes both the localized association of APC with microtubule plus ends and the assembly of Dlg containing puncta in the plasma membrane
Aceto et al., Dev Biol 2006
:
We conclude that the
CDC-42 interaction with PAR-6 is not required for the initial establishment of asymmetry but is
required for maximal cortical accumulation of
PAR-6 and to maintain its asymmetry
Atwood et al., J Cell Sci 2007
:
Conversely, expression of constitutively active
Cdc42 leads to ectopic
Par-6-aPKC localization and corresponding cell polarity defects