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EGF — PLD3
Text-mined interactions from Literome
Hornia et al., Mol Cell Biol 1999
(Cell Transformation, Neoplastic) :
Interestingly, both rottlerin and
EGF induced substantial
increases in
phospholipase D ( PLD ) activity, which is commonly elevated in response to mitogenic stimuli
Lu et al., Mol Cell Biol 2000
(Cell Transformation, Neoplastic) :
The
activation of
PLD by
EGF in these cells was dependent upon both Ras and RalA
Chen et al., IUBMB Life 2001
:
These results indicated that PKC, at least PKC-delta and Ca2+ dependent PKC-alpha or -betaI, plays an important role in BK-induced but not
EGF induced
PLD activation in A-431 cells
Min et al., Mol Cells 2001
(Carcinoma, Squamous Cell) :
Activation of
PLD by H2O2 and
EGF nearly correlated with tyrosine phosphorylation of the protein in PLD1 immune complexes
Andresen et al., Hypertension 2003
(MAP Kinase Signaling System) :
However,
EGF stimulation does not
activate phospholipase D or generate PA
Lee et al., J Biol Chem 2004
:
EGF treatment triggered the dissociation of Munc-18-1 from PLD when
PLD was
activated by
EGF ... The dissociation of the endogenous interaction between Munc-18-1 and PLD, and the
activation of
PLD by
EGF were also observed in primary cultured chromaffin cells
Cook et al., Biochem J 1992
:
Pre-treatment of Swiss 3T3 cells with the EGF-receptor tyrosine kinase inhibitor AG18 selectively inhibited
EGF stimulated
PLD activity ; bombesin stimulated PLD activity was unaffected
Wright et al., Biochem J 1992
:
Activation of
phospholipase D by alpha-thrombin or
epidermal growth factor contributes to the formation of phosphatidic acid, but not to observed increases in 1,2-diacylglycerol ... We investigated the
effect of alpha-thrombin and
epidermal growth factor (EGF) on cellular
PLD activity in order to determine the role of this enzyme in mitogen induced increases in phosphatidic acid and sn-1,2-diacylglycerol ... These data indicate that in quiescent IIC9 cells : ( a ) alpha-thrombin or
EGF rapidly and transiently
activates a
PLD ; ( b ) although this activation is responsible for part of the mitogen induced increases in phosphatidic acid, it does not contribute to induced increases in sn-1,2-diacylglycerol ; and ( c ) activation of this enzyme appears to be involved in the formation of a novel lipid generated in response to alpha-thrombin, but not EGF, in IIC9 fibroblasts
Fisher et al., J Cell Physiol 1991
:
To identify the presence of
EGF induced
phospholipase D activity, cells were labelled with exogenous [ 3H ] 1-0-hexadecyl, 2-acyl phosphatidylcholine and its conversion to phosphatidic acid in response to EGF determined ... Consistent with the presence of
EGF induced
phospholipase D activity, treatment of cells with EGF, in the presence of [ 14C ] ethanol, resulted in the rapid formation of [ 14C ] phosphatidylethanol, the product of phospholipase D-catalyzed transphosphatidylation
Morrison et al., J Cell Physiol 1995
:
Neither basic Fibroblast Growth Factor (bFGF) or
Epidermal Growth Factor (EGF) increased
PLD activity
Yeo et al., J Biol Chem 1995
:
Stimulation of
phospholipase D by
epidermal growth factor requires protein kinase C activation in Swiss 3T3 cells ...
EGF , basic fibroblast growth factor (bFGF), bombesin, and platelet derived growth factor ( PDGF )
activated PLD as measured by transphosphatidylation of butanol to phosphatidylbutanol ...
Activation of
PLD by
EGF was inhibited dose dependently by the PKC inhibitors bis-indolylmaleimide and Ro-31-8220, which also inhibited the effects of bFGF, bombesin, and PDGF ... In summary, all of the results of the study indicate that
PLD activation by
EGF is downstream of PtdIns ( 4,5 ) P2-hydrolyzing phospholipase and is dependent upon subsequent PKC activation
Dunlop et al., Biochim Biophys Acta 1993
:
We investigated the
activation of
phospholipase D ( PLD ) by a PKC activator, phorbol myristate acetate ( PMA ) and by
EGF in CHO cells transfected with the full-length EGF receptor
Budnik et al., FEBS Lett 1993
:
We propose, therefore, that phosphatidic acid may act as an intracellular second messenger linking
EGF mediated activation of
phospholipase D with the sensitization of LH receptor coupled adenylate cyclase signalling system
Mizunuma et al., Biochim Biophys Acta 1993
:
The
PLD was shown to be
activated by phorbol, 12-myristate, 13-acetate ( PMA ), calcium ionophore A23187, oxytocin, bombesin and bradykinin, but not by platelet activating factor (PAF) and
epidermal growth factor (EGF)
Kaszkin et al., Biochem J 1996
(Carcinoma, Squamous Cell) :
In the case of
EGF induced activation of a
phospholipase D the amount of phosphatidic acid is only slightly decreased in the presence of a primary alcohol
Hess et al., J Biol Chem 1997
:
In
response to
epidermal growth factor (EGF) ,
PLD activity was greatly increased in Rat1 fibroblasts expressing wild type Rac1 ( wtRac1 ), and completely abrogated in cells expressing dominant negative N17Rac1, consistent with Rac1 mediating the action of this growth factor ... In summary, these data indicate a major role for Rac1 in the
activation of
PLD by
EGF , but not PDGF or protein kinase C
Zhang et al., Curr Eye Res 1998
:
The aim of the present study was to investigate the
effect of
epidermal growth factor (EGF) on
PLD activity in rabbit corneal epithelial cells ( RCEC ) ... Under the same experimental conditions, the inhibitor had no effect on
EGF stimulated
PLD activity ... Down-regulation of PKC or treatment of the cells with RO31-8220, a PKC inhibitor, inhibited the PMA- but not
EGF stimulated
PLD activity ... Incubation of the cells with wortmannin, a PI 3-kinase inhibitor, abolished the EGF stimulated PI 3-kinase activity, but potentiated the
EGF stimulated
PLD activity ... These results indicate that
EGF stimulates
PLD activity in RCEC by a mechanism that involves tyrosine phosphorylation of a protein ( s ) in the cascade of biochemical reactions initiated by EGF-receptor interaction, and it is not dependent on concurrent activation of PKC, PLC, or PI 3-kinase in these cells
Carpio et al., Prostaglandins Leukot Essent Fatty Acids 1998
(Calcium Signaling) :
In summary, PA stimulates rat calvarial osteoblastic cell proliferation and mobilization of [ Ca2+ ] i using extracellular pools, and
EGF 's mitogenic effect on these cells
requires activation of
PLD
Carpio et al., J Bone Miner Res 1998
:
This study determined the
effects of
epidermal growth factor (EGF) on
PLD activity in normal rat osteoblastic cells