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CA2 — UTP3

Text-mined interactions from Literome

Gutierrez et al., Eur J Pharmacol 1999 : The aim of this investigation was to characterise the effects of ATP analogues and UTP on the single cell intracellular Ca2+ concentration ([Ca2+]i) in cultured rat mesangial cells
Hou et al., Biochem Biophys Res Commun 1999 : This was confirmed at the functional level by examining UTP stimulated release of intracellular Ca2+
Zünkler et al., Pharmacol Toxicol 1999 (Cardiomyopathy, Dilated) : Maximal [Ca2+ ] i responses of confluent human microvascular cardiac endothelial cell monolayers to UTP were lower when compared to 2MeSATP
Zhang et al., Respir Physiol 1999 : Our results indicate that both release of calcium from the intracellular store and Ca2+ influx across the cell membrane contribute to the mobilization of [Ca2+ ] i upon stimulation with nucleotides, that ATP and UTP regulate intracellular Ca2+ predominantly via the G protein-phospholipase C-IP3 pathway, and that ATP and UTP may act via distinct subtypes of P2Y receptors
Szárics et al., Acta Pharm Hung 1999 : Also, the activation of the fast-phase Ca2+ ion response by ATP, UDP and alpha, beta-methylene-ATP whereas that of the slow-phase by UTP and UDP were observed with 3 microM concentration of these P2 receptor ligands
Arslan et al., Neuropharmacology 2000 : UTP caused an increase in intracellular Ca2+ , and InsP3 formation could be stimulated by ATP and UTP
Liu et al., J Physiol 2000 : ATP- and UTP induced rises in [Ca2+ ] i were completely and reversibly blocked by 10 microM PPADS ( a P2 purinoceptor antagonist ) and partially inhibited by 100 microM suramin ( a relatively non-specific purinoceptor antagonist )
Sabala et al., Br J Pharmacol 2001 (Brain Neoplasms...) : 4. Suramin, a non-specific nucleotide receptors inhibitor, antagonized ATP-, UTP- and ADP evoked Ca2+ responses
Moccia et al., Microvasc Res 2001 (Calcium Signaling) : UTP increased the intracellular [Ca2+ ] in 94 % of the cells, whereas 2MeSATP was active in 32 %
Welsh et al., Am J Physiol Heart Circ Physiol 2001 : UTP also depolarized and increased cytosolic Ca2+ in coronary smooth muscle cells
Marrelli et al., Stroke 2002 (Brain Ischemia...) : I/R resulted in significantly potentiated UTP mediated dilations ( through a P2Y2 purinoceptor ) and endothelial Ca2+ responses in the presence of N ( G ) -nitro-L-arginine methyl ester ( L-NAME ) and indomethacin
White et al., Mol Pharmacol 2003 : Unexpectedly, however, both UTP and ATP stimulated increases in cytosolic Ca2+ concentration ([Ca2+]c) , with both nucleotides achieving similar EC50 and maximal responses ... The [ Ca2+ ] c increase in response to UTP was less dependent on extracellular Ca2+ than was the response to ATP
Juul et al., J Vasc Res 1992 : Effects of ATP and UTP on [Ca2+ ] i, membrane potential and force in isolated rat small arteries ... We have investigated excitation-contraction coupling mechanisms associated with the activation of purinoceptors and putative pyrimidinoceptors by assessing the effects of ATP and UTP on cytoplasmic Ca2+ activity ([Ca2+]i) , membrane potential ( Em ) and force in rat mesenteric small arteries
Marsault et al., Biochem Biophys Res Commun 1992 : Finally, ATP, UTP and ADP increases [Ca2+ ] i in aortic fibroblasts via a nucleotide receptor that has a higher affinity for ATP and UTP ( 3 microM ) than for ADP ( 50 microM ) and that is distinct from P2x and P2y purinoceptors
Choi et al., Acta Otolaryngol 2003 : UTP and ATPgammaS increased [ Ca2+ ] i via caffeine-sensitive pathways, and these two agonists stimulated mucin secretion to a similar magnitude without their gene enhancement
Pitt et al., Mol Pharmacol 2005 (Calcium Signaling) : Elevated K+ also amplified endogenous UTP dependent Ca2+ responses in human embryonic kidney 293 cells, suggesting that other P2Y receptors are K ( + ) -dependent
Collison et al., Exp Eye Res 2005 : Both ATP and UTP ( 10 microM ) increased [Ca2+ ] i in human RPE cells
Sugama et al., Mol Pharmacol 2005 : Mastoparan inhibited UTP induced increase in [Ca2+ ] i and phosphoinositide hydrolysis in a concentration dependent manner
del Rey et al., J Biol Chem 2006 (Calcium Signaling) : In Ca2+-free buffer, the potent ( K0.5 < 1 microm ) stimulatory effect of UTP ( or ATP ) on endoplasmic reticulum ( ER ) Ca2+ release was abolished in cells isolated from P2Y2/P2Y4 double knock-out mice
Ross et al., Br J Dermatol 2008 : Both ATP and UTP induced Ca2+ release in primary human keratinocytes
Wang et al., Cell Calcium 2009 (Calcium Signaling) : Unlike triadin knockdown myotubes, junctin knockdown and junctin/triadin double knockdown myotubes also had reduced Ca2+ release induced by 400 microM 4-chloro-m-cresol, 10mM caffeine, 400 microM UTP , or 1 microM thapsigargin
Ohnishi et al., Molecular pain 2009 (Pain) : ATP, 2-MeSATP, and UTP increased the intracellular Ca2+ concentration ([Ca2+]i) in PC12 cells
Agutter et al., Biochem J 1979 : All three preparations hydrolyse ATP, 2'-dATP, 3'-dATP, GTP, CTP and UTP in the presence of Mg2+, Ca2+ , Mn2+ and Co2+ with a pH optimum of 8.0, are sensitive to inhibition by mercurials, arsenicals, quercetin, proflavin and adenosine 5'- [ gamma-thio ] triphosphate and are partially inactivated by exposure to high ionic strength
Häussinger et al., Eur J Biochem 1987 : With 1.25 mM Ca2+ , UTP induced Ca2+ movements only near the detection level ( i.e. below 10-20 nmol/g liver ) ; however, with 0.3 mM Ca2+ in influent perfusate, there was a slow Ca2+ release ( not completed within 5-6 min )
Wiley et al., Biochem J 1995 (Leukemia, Lymphocytic, Chronic, B-Cell) : In contrast, ATP or UTP ( 1-10 microM ) had no effect on the cytosolic Ca2+ of lymphocytes attached to glass
Schöfl et al., Biochem Biophys Res Commun 1995 : In single human gastric mucous cells extracellular ATP, ATP-gamma-S, ADP and UTP at micromolar concentrations caused a biphasic increase in [Ca2+ ] i. Consistent with a P2u-receptor the rank-order of potency was ATP > or = UTP > ATP-gamma-S > ADP, and cross-desensitization of the Ca2+ responses occurred between ATP and UTP
Schöfl et al., Biochem Biophys Res Commun 1995 : In single human thyrocytes extracellular ATP, ATP-gamma-S, ADP and UTP caused a concentration dependent biphasic increase in [Ca2+ ] i. Consistent with a P2u-receptor the rank-order of potency was ATP = UTP > ATP-gamma-S > ADP, and cross-desensitization of the Ca2+ responses occurred between ATP and UTP
Alonso-Torre et al., Pflugers Arch 1995 : Both uridine 5'-triphosphate ( UTP ) and platelet activating factor (PAF) triggered the release of Ca2+ followed by a sustained influx, during which intracellular stores remained totally empty
Matsuoka et al., Mol Pharmacol 1995 (Glioma...) : In contrast, beta, gamma-methylene-ATP selectively elicited cAMP accumulation, whereas 2-methylthio-ATP and UTP induced only Ca2+ mobilization, without affecting cAMP levels
Petit et al., J Clin Endocrinol Metab 1995 (Ion Channel Gating) : The pharmacological activation profile suggests the presence of purinergic P2u receptors ( nucleotide receptors ), because uridine 5'-triphosphate ( UTP ) also stimulated [ Ca2+ ] i ( 4.0-fold increase, with an EC50 of 10 mumol/L )
Sipma et al., Br J Pharmacol 1995 : Regulation of histamine- and UTP induced increases in Ins ( 1,4,5 ) P3, Ins ( 1,3,4,5 ) P4 and Ca2+ by cyclic AMP in DDT1 MF-2 cells ... 5. Forskolin and isoprenaline reduced the histamine induced increase in cytoplasmic Ca2+, as measured in Ca2+ containing medium and in nominally Ca ( 2+ ) -free medium but did not change the UTP induced increase in cytoplasmic Ca2+
Musgrave et al., Biochem Pharmacol 1994 : No effect of alpha 2-adrenoceptor agonists was seen on either fMLP induced O2- production or UTP induced increases in [Ca2+ ] i. alpha 2-Adrenoceptor agonists by themselves had no effect on either O2- production or [Ca2+ ] i ... While the alpha 2-adrenoceptor agonists 5-bromo-6- ( 2-imidazolin-2-ylamino ) -quinoxaline ( UK 14304 ) and 5-allyl-2-amino-5,6,7,8-tetrahydro-4H-thiazolo- [ 4,5-d ] azepin- dihydrochloride increased the [Ca2+ ] i in HEL cells, they had no effect by themselves on either [Ca2+ ] i or UTP induced increases in [Ca2+ ] i in differentiated HL-60 cells
Wilson et al., Exp Physiol 1994 : UTP and ADP also increased [ Ca2+ ] i, whereas adenosine, AMP and alpha, beta-methylene ATP were without effect
Henning et al., Br J Pharmacol 1993 : 6. Stimulation of the cells with ATP or UTP induced a rapid increase in intracellular Ca2+ , followed by a slow decline to basal levels
Majid et al., Biochem Biophys Res Commun 1993 : ATP, its derivatives ( 2-methylthioadenosine 5'-triphosphate ( MeSATP ) and 2'- and 3'-O- ( 4-benzoylbenzoyl ) adenosine 5'-triphosphate ( BzATP ) ) and UTP increased intracellular Ca2+ in the presence of 2 mM extracellular Ca2+ with the potency order of ATP > MeSATP > BzATP = UTP
Lin et al., J Neurochem 1993 (Glioma...) : Similar to ATP, UTP also induced polyphosphoinositide breakdown, inositol phosphate formation, and an increase in [ Ca2+ ] i. ADP, ITP, TTP, GTP, ATP gamma S, 2-methylthio ATP, beta, gamma-imidoATP or 3'-O- ( 4-benzoyl ) benzoylATP, but not CTP, AMP, beta, gamma-methylene ATP, or adenosine, also caused an increase in [ Ca2+ ] i
Frelin et al., J Biol Chem 1993 : ATP, UTP , and adenosine 5'-O- ( 3-thiotrisphosphate ) activated phospholipase C and induced large increases in [Ca2+ ] i. ADP had a different action
Enomoto et al., Cell Biochem Funct 1995 (Mammary Neoplasms, Experimental) : Bradykinin, ATP, UTP and UDP induced an increase of intracellular Ca2+ and the release of arachidonic acid from phospholipids into the extracellular fluid
Miyagi et al., Biochem Biophys Res Commun 1996 : UTP induced a rise in the cytosolic Ca2+ concentration ([Ca2+]i) of VSMCs, which was desensitized by pretreatment with ATP, but not with 2-methylthioATP nor alpha, beta-methyleneATP
Ho et al., Br J Pharmacol 1995 : These findings indicate that UTP stimulates the release of Ca2+ from a thapsigargin-sensitive intracellular pool ... 4. The Ca2+ response to UTP was unaffected by treatment with pertussis toxin, suggesting that UTP responses may be mediated via a pertussis toxin-insensitive G protein
Filippov et al., Eur J Neurosci 1996 (Neuroblastoma) : We now report that UTP also inhibits Ca2+-currents in differentiated NG108-15 cells, but probably through a different nucleotide receptor
Miyagi et al., Br J Pharmacol 1996 : In the absence of extracellular Ca2+, UTP induced only the initial transient increases in [ Ca2+ ] i and force, while the sustained increases in [ Ca2+ ] i and force were abolished
Strøbaek et al., Br J Pharmacol 1996 : alpha, beta-MeATP was without effect, whereas 2MeSATP and UTP induced release of Ca2+ from internal stores with 2MeSATP being the most potent agonist ( EC50 = 0.17 microM ), and UTP having a potency similar to ATP ... Since a release of Ca2+ was induced by 2MeSATP as well as by UTP , the data indicate that P2y- as well as P2U-purinoceptors are expressed by the HCASMC
Chen et al., J Cardiovasc Pharmacol 1996 : Stimulation of the cells with either 2MeSATP or UTP induced a rapid increase in intracellular Ca2+ level, followed by a slow decrease to basal levels, followed by Ca2+ level oscillation
King et al., Neuroscience 1996 : Both UTP and ATP increased intracellular Ca2+ ( as measured by fura-2/AM luminescence ) which, in cross-desensitization experiments, indicated the involvement of two subtypes of P2 purinoceptors
Shahidullah et al., Curr Eye Res 1997 : In the presence of extracellular Ca2+ , ATP, UTP or ADP induced a transient dose dependent increase in intracellular calcium ( maximum approx. 400 % ), which declined rapidly
Fukushi et al., Eur J Pharmacol 1997 : UTP , acting through P2U purinoceptors, causes Ca2+ release independent of external Na+
Murthy et al., J Biol Chem 1998 : UTP and ATP stimulated inositol 1,4,5-triphosphate formation, Ca2+ release, and contraction that were abolished by U-73122 and guanosine 5'-O- ( 3-thio ) diphosphate, and partly inhibited ( 50-60 % ) by pertussis toxin ( PTX )
Wilkinson et al., Eur J Pharmacol 1997 : SRIF ( pEC50 7.10 ) and UTP ( pEC50 ) 5.14 ) caused concentration dependent increases in [ Ca2+ ] i but the SRIF maximum was about 40 % of that to UTP
Jan et al., Chin J Physiol 1998 : Both the peak height and under-curve area of 10 microM UTP induced Ca2+ signal was reduced by approximately 40 % by extracellular Ca2+ removal, suggesting that UTP induced capacitative Ca2+ entry ... La3+ inhibited the UTP induced Ca2+ signal dose-dependently when added before or after UTP
Nguyen et al., Am J Physiol 1998 : We observed that 1 ) ATP, UTP , adenosine 5'-O- ( 3-thiotriphosphate ), and, to a lesser extent, beta, gamma-methylene-ATP, but not adenosine, stimulated 125I- efflux from PDEC, suggesting a primary role for P2Y2 receptors, 2 ) ATP stimulated 125I- efflux was inhibited by 5-nitro-2- ( 3-phenylpropylamino ) benzoic acid, diphenylamine-2-carboxylate, and DIDS, suggesting mediation through Ca2+ activated Cl- channels, 3 ) ATP stimulated an 86Rb+ efflux sensitive to BaCl2 and charybdotoxin, thus likely occurring through Ca2+ activated K+ channels, 4 ) serosal or luminal addition of UTP activated apical Cl- conductance and basolateral K+ conductance when nystatin permeabilized PDEC were studied in an Ussing chamber, suggesting the expression of P2Y2 receptors on both sides of the cell, 5 ) ATP stimulated mucin secretion, and 6 ) ATP increases intracellular Ca2+ concentration ([Ca2+]i)
Toescu et al., Neuroscience 1998 : The ATP induced Ca2+ response involved both Ca2+ influx through ionotropic receptors and Ca2+ release from intracellular pools, whereas UTP selectively stimulated intracellular Ca2+ release
Ohkubo et al., Naunyn Schmiedebergs Arch Pharmacol 1998 : The pretreatment with FSBA did not affect the increase in [Ca2+ ] i induced by ATP, UTP or benzoylbenzoic ATP
vom Dahl et al., Hepatology 1999 : Single-cell fluorescence studies with isolated liver PC, Kupffer cells ( KC ), sinusoidal endothelial cells ( SEC ), and hepatic stellate cells (HSC) revealed that phenylephrine induced an increase in cytosolic free Ca2+ only in PC and HSC, but not in KC and SEC, whereas extracellular uridine triphosphate ( UTP ) produced Ca2+ transients/oscillations in all liver cell types studied
Catinot et al., Eur J Pharmacol 1998 : The presence of ryanodine ( 200 microM ), ruthenium red ( 10 microM ) or heparin ( 2.5 mg/ml ) did not affect the tension elicited in the presence of UTP, demonstrating that the UTP induced Ca2+ release involved neither ryanodine nor inositol triphosphate-sensitive channels
Inscho et al., Hypertension 1999 : The effect of ATP and UTP on [Ca2+ ] i was studied on single cells with standard microscope based fluorescence photometry techniques ... In contrast, exposure to 100 micromol/L UTP under calcium-free conditions resulted in no detectable change in the UTP mediated increase in [Ca2+ ] i ... These data demonstrate that both ATP and UTP directly stimulate a biphasic increase in [ Ca2+ ] i in renal microvascular smooth muscle cells