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SMAD3 — SMURF2

Pathways - manually collected, often from reviews:

• NCI Pathway Database TGF-beta receptor signaling: SMAD7/SMURF1-2 complex (SMAD7-SMURF1_SMURF2) → TGFB/TGFBR2/TGFBR1/PML/SARA/SMAD2-3/AP2B2 complex (TGFBR1-PML-ZFYVE9-SMAD2_SMAD3-TGFBR2) (modification, collaborate)
  Wu et al., Science 2000, Yao et al., J Biol Chem 2000, Kavsak et al., Mol Cell 2000, Ebisawa et al., J Biol Chem 2001, Huse et al., Mol Cell 2001, Lu et al., J Biol Chem 2002, Suzuki et al., J Biol Chem 2002, Hayes et al., J Cell Biol 2002, Yao et al., Mol Biol Cell 2002, Di Guglielmo et al., Nat Cell Biol 2003, Lin et al., Nature 2004, Hayashi et al., Cell 1997, Nakao et al., Nature 1997, Abdollah et al., J Biol Chem 1997, Lo et al., EMBO J 1998, Chen et al., Genes Dev 1998, Tsukazaki et al., Cell 1998
  Evidence: mutant phenotype, assay, physical interaction, other species
• NCI Pathway Database TGF-beta receptor signaling: SMAD7/SMURF1-2 complex (SMAD7-SMURF1_SMURF2) → PML/SARA/SMAD2-3 complex (ZFYVE9-SMAD2_SMAD3-PML) (modification, collaborate)
  Wu et al., Science 2000, Yao et al., J Biol Chem 2000, Kavsak et al., Mol Cell 2000, Ebisawa et al., J Biol Chem 2001, Huse et al., Mol Cell 2001, Lu et al., J Biol Chem 2002, Suzuki et al., J Biol Chem 2002, Hayes et al., J Cell Biol 2002, Yao et al., Mol Biol Cell 2002, Di Guglielmo et al., Nat Cell Biol 2003, Lin et al., Nature 2004, Hayashi et al., Cell 1997, Nakao et al., Nature 1997, Abdollah et al., J Biol Chem 1997, Lo et al., EMBO J 1998, Chen et al., Genes Dev 1998, Tsukazaki et al., Cell 1998
  Evidence: mutant phenotype, assay, physical interaction, other species
• NCI Pathway Database TGF-beta receptor signaling: TGFB/TGFBR2/TGFBR1/PML/SARA/SMAD2-3/AP2B2 complex (TGFBR1-PML-ZFYVE9-SMAD2_SMAD3-TGFBR2) → TGFB/TGFBR2/TGFBR1/SMAD7/SMURF1-2/CAV1 complex (TGFBR1-SMAD7-SMURF1_SMURF2-CAV1-TGFBR2) (modification, collaborate)
  Wu et al., Science 2000, Yao et al., J Biol Chem 2000, Kavsak et al., Mol Cell 2000, Ebisawa et al., J Biol Chem 2001, Huse et al., Mol Cell 2001, Lu et al., J Biol Chem 2002, Suzuki et al., J Biol Chem 2002, Hayes et al., J Cell Biol 2002, Yao et al., Mol Biol Cell 2002, Di Guglielmo et al., Nat Cell Biol 2003, Lin et al., Nature 2004, Hayashi et al., Cell 1997, Nakao et al., Nature 1997, Abdollah et al., J Biol Chem 1997, Lo et al., EMBO J 1998, Chen et al., Genes Dev 1998, Tsukazaki et al., Cell 1998
  Evidence: mutant phenotype, assay, physical interaction, other species
• NCI Pathway Database TGF-beta receptor signaling: TGFB/TGFBR2/TGFBR1/SMAD7/SMURF1-2/CAV1 complex (TGFBR1-SMAD7-SMURF1_SMURF2-CAV1-TGFBR2) → PML/SARA/SMAD2-3 complex (ZFYVE9-SMAD2_SMAD3-PML) (modification, collaborate)
  Wu et al., Science 2000, Yao et al., J Biol Chem 2000, Kavsak et al., Mol Cell 2000, Ebisawa et al., J Biol Chem 2001, Huse et al., Mol Cell 2001, Lu et al., J Biol Chem 2002, Suzuki et al., J Biol Chem 2002, Hayes et al., J Cell Biol 2002, Yao et al., Mol Biol Cell 2002, Di Guglielmo et al., Nat Cell Biol 2003, Lin et al., Nature 2004, Hayashi et al., Cell 1997, Nakao et al., Nature 1997, Abdollah et al., J Biol Chem 1997, Lo et al., EMBO J 1998, Chen et al., Genes Dev 1998, Tsukazaki et al., Cell 1998
  Evidence: mutant phenotype, assay, physical interaction, other species
• Reactome Reaction: SMAD3 → SMURF2 (indirect_complex) Tang et al., EMBO J 2011
• Reactome Reaction: SMAD3 → SMURF2 (reaction) Tang et al., EMBO J 2011

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

• IRef Bind_translation Interaction: SMAD3 — SMURF2 (coimmunoprecipitation) Barrios-Rodiles et al., Science 2005
• IRef Biogrid Interaction: SMURF2 — SMAD3 (physical association, affinity chromatography technology) Gao et al., Mol Cell 2009
• IRef Biogrid Interaction: SMURF2 — SMAD3 (physical association, affinity chromatography technology) Tang et al., EMBO J 2011
• IRef Biogrid Interaction: SMURF2 — SMAD3 (physical association, affinity chromatography technology) Brown et al., J Cell Biochem 2008
• IRef Biogrid Interaction: SMURF2 — SMAD3 (direct interaction, two hybrid) Lin et al., J Biol Chem 2000
• IRef Biogrid Interaction: SMURF2 — SMAD3 (direct interaction, pull down) Lin et al., J Biol Chem 2000
• IRef Biogrid Interaction: SMURF2 — SMAD3 (physical association, affinity chromatography technology) Bonni et al., Nat Cell Biol 2001
• IRef Biogrid Interaction: SMURF2 — SMAD3 (physical association, affinity chromatography technology) Nakano et al., J Biol Chem 2009*
• IRef Biogrid Interaction: SMURF2 — SMAD3 (physical association, affinity chromatography technology) Komuro et al., Oncogene 2004
• IRef Biogrid Interaction: SMURF2 — SMAD3 (physical association, affinity chromatography technology) Zhang et al., Proc Natl Acad Sci U S A 2001
• IRef Biogrid Interaction: SMURF2 — SMAD3 (direct interaction, two hybrid) Zhang et al., Proc Natl Acad Sci U S A 2001
• MIPS CORUM SMURF2-SMAD3-SnoN complex, TGF(beta)-dependent: SMURF2-SMAD3-SnoN complex, TGF(beta)-dependent complex (SKIL-SMAD3-SMURF2) Bonni et al., Nat Cell Biol 2001
• IRef Corum Interaction: Complex of SMAD3-SKIL-SMURF2 (association, anti bait coimmunoprecipitation) Bonni et al., Nat Cell Biol 2001
• IRef Corum Interaction: SMAD3 — SMURF2 (association, anti bait coimmunoprecipitation) Bonni et al., Nat Cell Biol 2001
• IRef Hprd Interaction: SMURF2 — SMAD3 (in vitro) Lin et al., J Biol Chem 2000
• IRef Hprd Interaction: SMURF2 — SMAD3 (two hybrid) Lin et al., J Biol Chem 2000
• IRef Intact Interaction: Complex of 48 proteins (physical association, tandem affinity purification) Brown et al., J Cell Biochem 2008
• IRef Intact Interaction: Complex of 43 proteins (physical association, tandem affinity purification) Brown et al., J Cell Biochem 2008
• IRef Intact Interaction: SMAD3 — SMURF2 (physical association, anti tag coimmunoprecipitation) Tang et al., EMBO J 2011
• IRef Intact Interaction: SMAD3 — SMURF2 (direct interaction, isothermal titration calorimetry) Tang et al., EMBO J 2011
• IRef Intact Interaction: SMAD3 — SMURF2 (physical association, peptide array) Tang et al., EMBO J 2011
• IRef Ophid Interaction: SMAD3 — SMURF2 (aggregation, interologs mapping) Brown et al., Bioinformatics 2005

Text-mined interactions from Literome

Lin et al., J Biol Chem 2000 : Ectopic expression of Smurf2 was sufficient to reduce the steady-state levels of Smad1 and Smad2 but not Smad3 or Smad4
Ohashi et al., FEBS Lett 2005 : TGF-beta mediated Smurf2 promoter activation was inhibited by Smad7 or an activin receptor-like kinase 5 inhibitor but not by dominant negative Smad or disruption of Smad binding elements in the promoter
Tan et al., American journal of physiology. Renal physiology 2008 (Chronic Disease...) : Ectopic expression of Smurf2 was sufficient to reduce the steady-state levels of Smad2, but not Smad1, Smad3 , Smad4, and Smad7, in HKC-8 cells
Zhang et al., Burns Incl Therm Inj 2012 (Burns...) : Smad proteins are important intracellular mediators of TGF-ß1 signaling, and Smad ubiquitination regulatory factor 2 ( Smurf2 ), an ubiquitin ligase for Smads, plays critical roles in the regulation of TGF-ß1/Smad signaling
Tang et al., EMBO J 2011 : Thus, our data support a model in which Smurf2 negatively regulates TGF-ß signalling by attenuating the activity of Smad3 rather than promoting its degradation