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MYLK — TNF

Text-mined interactions from Literome

Petrache et al., FASEB J 2003 : TNF-alpha induced the caspase dependent cleavage of EC MLCK-1745 in transfected endothelial cells, which was confirmed by mass spectroscopy with in vitro cleavage by caspase 3 at LKKD ( D1703 )
Wadgaonkar et al., J Cell Biochem 2005 : We previously demonstrated that increased EC contractility, rearrangement of the actin cytoskeleton, and increased myosin light chain ( MLC ) phosphorylation occurs as a consequence of TNFalpha induced activation of EC MLC kinase ( EC MLCK ) and is required for bovine lung EC apoptosis
Wang et al., Am J Pathol 2005 : However, at doses below those needed for nuclear factor-kappaB inhibition, sulfasalazine was able to prevent TNF-alpha induced barrier dysfunction, MLCK up-regulation, and MLC phosphorylation
Wadgaonkar et al., J Cell Biochem 2005 : This EC MLCK-MIF interaction was shown biochemically and by immunofluorescent microscopy to be enhanced in TNFalpha- and thrombin stimulated endothelium, both of which induce increased MLCK activity
Ye et al., Am J Physiol Gastrointest Liver Physiol 2006 : The TNF-alpha induced increase in MLCK transcription corresponded to a sequential increase in MLCK protein expression, MLCK activity, and Caco-2 TJ permeability ... The TNF-alpha induced increase in MLCK promoter activity was mediated by NF-kappaB activation, and the inhibition of NF-kappaB activation prevented the TNF-alpha induced increase in promoter activity and the subsequent increase in MLCK protein expression and Caco-2 TJ permeability ... The TNF-alpha induced activation of MLCK promoter was mediated by binding of the activated NF-kappaB p50/p65 dimer to the downstream kappaB binding site ( -84 to -75 ) on the MLCK promoter region ; deletion of the kappaB binding site prevented the TNF-alpha increase in promoter activity
Graham et al., J Biol Chem 2006 : Tumor necrosis factor induced long myosin light chain kinase transcription is regulated by differentiation dependent signaling events ... We found that TNF increases long MLCK mRNA transcription, both in human enterocytes in vitro and murine enterocytes in vivo.5'-RACE identified two novel exons, 1A and 1B, which encode alternative long MLCK transcriptional start sites ... Analysis of differentiating epithelia showed that only well differentiated enterocytes activated the 4-kb long MLCK promoter in response to TNF , and consensus promoter reporters demonstrated that TNF induced NFkappaB activation decreased during differentiation while TNF induced AP-1 activation increased
Wang et al., Gastroenterology 2006 (Intestinal Diseases) : In vitro studies showed that TNF caused similar increases in MLCK expression and MLC phosphorylation, as well as barrier dysfunction, in Caco-2 monolayers only after interferon (IFN)-gamma pretreatment
Boivin et al., Am J Physiol Gastrointest Liver Physiol 2007 : Glucocorticoids inhibited the TNF-alpha induced increase in myosin light chain kinase (MLCK) protein expression, a key process mediating the TNF-alpha increase in intestinal TJ permeability ... The prednisolone protective action was mediated by binding of activated GR complex to the GRE site on the MLCK promoter, suppressing the TNF-alpha induced increase in MLCK gene activity, protein expression, and subsequent opening of the intestinal TJ barrier
Song et al., J Pharmacol Exp Ther 2008 (Chronic Disease...) : KRP-203 obviously inhibited the production of interferon-gamma, IL-12, and tumor necrosis factor-alpha by the colonic lymphocytes, but had no influence on IL-4 production
Ye et al., J Cell Mol Med 2008 : Previous studies have shown that TNF-alpha increase in TJ permeability was regulated by an increase in myosin light chain kinase (MLCK) gene activity and protein expression ... The major aim of this study was to elucidate the cellular and molecular mechanisms that mediate basal and TNF-alpha induced increase in MLCK gene activity ... TNF-alpha induced increase in MLCK promoter activity was mediated by NF-kappaB activation ... In conclusion, we have identified the minimal MLCK promoter region, essential molecular determinants and molecular mechanisms that mediate basal and TNF-alpha induced modulation of MLCK promoter activity in Caco-2 intestinal epithelial cells ... These studies provide novel insight into the cellular and molecular mechanisms that regulate basal and TNF-alpha induced modulation of MLCK gene activity
Costantini et al., J Trauma 2009 (Burns) : Prior studies have shown that tumor necrosis factor (TNF)-alpha activates MLCK , in part through a nuclear factor (NF)-kappa B-dependent pathway
Liu et al., Cell Physiol Biochem 2012 : The mechanism by which oligomycin protects intestinal barrier function may, at least in part, be attributed to block the up-regulated MLCK transcription and protein expression induced by IFN-? and TNF-a
Shen et al., Ann N Y Acad Sci 2012 : Tumor necrosis factor ( TNF-a ) causes a myosin light chain kinase (MLCK) -- mediated barrier regulation by inducing occludin removal from the tight junction through caveolar endocytosis
Adyshev et al., Am J Respir Cell Mol Biol 2013 (Acute Lung Injury...) : We identified increased MYLK gene transcription induced by TNF-a ( 24 h ; 4.7 ± 0.45 fold increase [ FI ] ), LPS ( 4 h ; 2.85 ± 0.15 [ FI ] ), and 18 % cyclic stretch ( 24 h ; 4.6 ± 0.24 FI ) that was attenuated by transfection of human lung ECs with mimics of hsa-miR-374a, hsa-miR-374b, hsa-miR-520c-3p, or hsa-miR-1290 ( 20-80 % reductions by each miRNA )
Potì et al., Arterioscler Thromb Vasc Biol 2013 (Aortic Diseases...) : In vitro studies demonstrated that KRP-203 reduced tumor necrosis factor-a , interleukin-6, and interferon-? induced protein-10 production ; I?B and signal transducer and activator of transcription-1 phosphorylation ; and nuclear factor ?B and signal transducer and activator of transcription-1 activation in poly ( I:C ) -, lipopolysaccharide-, or interferon-? stimulated bone marrow macrophages, respectively
Cao et al., PloS one 2013 : The increase of both MLC phosphorylation and MLCK protein expression induced by IFN-? and TNF-a was significantly inhibited by berberine treatment
Wright et al., J Cell Biochem 1993 (Breast Neoplasms...) : TNF activation of DNA fragmentation was blocked by a potent inhibitor of myosin light chain kinase (MLCK) but was unaffected by inhibitors of cAMP or cGMP dependent PKs