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GRB10 — INSR

Pathways - manually collected, often from reviews:

• OpenBEL Selventa BEL large corpus: GRB10 → INSR (directlyIncreases, GRB10 Activity)
  Evidence: It also contains an additional binding site located in the phosphorylated kinase activation loop of the insulin receptor but is only very slightly phosphorylated by insulin receptor.
• NCI Pathway Database Insulin Pathway: GRB10 (GRB10) → Insulin Receptor/Insulin/SHC complex (INSR-INS-SHC1) (modification, collaborate)
  O'Neill et al., J Biol Chem 1996, Dong et al., Mol Endocrinol 1997*
  Evidence: physical interaction
• NCI Pathway Database Insulin Pathway: GRB10 (GRB10) → Insulin Receptor/Insulin/SHC/GRB10 complex (INSR-INS-SHC1-GRB10) (modification, collaborate)
  O'Neill et al., J Biol Chem 1996, Dong et al., Mol Endocrinol 1997*
  Evidence: physical interaction
• NCI Pathway Database Insulin Pathway: Insulin Receptor/Insulin/SHC complex (INSR-INS-SHC1) → Insulin Receptor/Insulin/SHC/GRB10 complex (INSR-INS-SHC1-GRB10) (modification, collaborate)
  O'Neill et al., J Biol Chem 1996, Dong et al., Mol Endocrinol 1997*
  Evidence: physical interaction
• NCI Pathway Database Insulin Pathway: Insulin Receptor/Insulin/SHC/GRB10 complex (INSR-INS-SHC1-GRB10) → p62DOK (DOK1) (modification, inhibits) Wick et al., J Biol Chem 2001
  Evidence: assay
• NCI Pathway Database Insulin Pathway: Insulin Receptor/Insulin/SHC/GRB10 complex (INSR-INS-SHC1-GRB10) → Insulin Receptor/Insulin/SHC/GRB2/Sos1 complex (INSR-INS-SHC1-GRB2-SOS1) (modification, inhibits)
  Wick et al., J Biol Chem 2001
  Evidence: assay
• Reactome Reaction: GRB10 → INSR (indirect_complex) Wick et al., J Biol Chem 2003*, Langlais et al., Mol Endocrinol 2004*
• Reactome Reaction: GRB10 → INSR (reaction) Wick et al., J Biol Chem 2003*, Langlais et al., Mol Endocrinol 2004*

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

• IRef Bind Interaction: GRB10 — INSR He et al., J Biol Chem 1998
• IRef Bind_translation Interaction: GRB10 — INSR (affinity chromatography technology) He et al., J Biol Chem 1998
• IRef Bind_translation Interaction: GRB10 — INSR (two hybrid) He et al., J Biol Chem 1998
• IRef Biogrid Interaction: GRB10 — INSR (direct interaction, two hybrid) Hansen et al., J Biol Chem 1996*
• IRef Biogrid Interaction: GRB10 — INSR (direct interaction, pull down) Hansen et al., J Biol Chem 1996*
• IRef Biogrid Interaction: GRB10 — INSR (physical association, affinity chromatography technology) Hansen et al., J Biol Chem 1996*
• IRef Biogrid Interaction: GRB10 — INSR (direct interaction, two hybrid) Liu et al., Proc Natl Acad Sci U S A 1995*
• IRef Biogrid Interaction: GRB10 — INSR (physical association, affinity chromatography technology) Liu et al., Proc Natl Acad Sci U S A 1995*
• IRef Biogrid Interaction: GRB10 — INSR (direct interaction, pull down) Liu et al., Proc Natl Acad Sci U S A 1995*
• IRef Biogrid Interaction: GRB10 — INSR (direct interaction, two hybrid) He et al., J Biol Chem 1998
• IRef Biogrid Interaction: GRB10 — INSR (direct interaction, pull down) He et al., J Biol Chem 1998
• IRef Biogrid Interaction: GRB10 — INSR (physical association, affinity chromatography technology) Frantz et al., J Biol Chem 1997*
• IRef Biogrid Interaction: GRB10 — INSR (physical association, affinity chromatography technology) Mori et al., Mol Cell Endocrinol 2005*
• IRef Biogrid Interaction: GRB10 — INSR (physical association, affinity chromatography technology) Langlais et al., Oncogene 2000*
• IRef Dip Interaction: GRB10 — INSR (physical association, anti bait coimmunoprecipitation) Depetris et al., Nature structural & molecular biology 2009*
• IRef Hprd Interaction: GRB10 — INSR (in vitro) Wick et al., J Biol Chem 2003*, Liu et al., Proc Natl Acad Sci U S A 1995*, Hansen et al., J Biol Chem 1996*, He et al., J Biol Chem 1998
• IRef Hprd Interaction: GRB10 — INSR (two hybrid) Wick et al., J Biol Chem 2003*, Liu et al., Proc Natl Acad Sci U S A 1995*, Hansen et al., J Biol Chem 1996*, He et al., J Biol Chem 1998
• IRef Hprd Interaction: GRB10 — INSR (in vivo) Wick et al., J Biol Chem 2003*, Liu et al., Proc Natl Acad Sci U S A 1995*, Hansen et al., J Biol Chem 1996*, He et al., J Biol Chem 1998
• IRef Innatedb Interaction: GRB10 — INSR (unknown, -) Wick et al., J Biol Chem 2003*
• IRef Intact Interaction: GRB10 — INSR (direct interaction, pull down) He et al., J Biol Chem 1998
• IRef Intact Interaction: GRB10 — INSR (physical association, protein three hybrid) He et al., J Biol Chem 1998
• IRef Intact Interaction: GRB10 — INSR (physical association, two hybrid) He et al., J Biol Chem 1998
• IRef Ophid Interaction: GRB10 — INSR (aggregation, interologs mapping) Brown et al., Bioinformatics 2005
• IRef Ophid Interaction: GRB10 — INSR (aggregation, confirmational text mining) Hansen et al., J Biol Chem 1996*

Text-mined interactions from Literome

Holt et al., Biochem J 2005 (Genetic Predisposition to Disease) : Grb10 and Grb14, in particular, have been implicated in the regulation of insulin receptor signalling, whereas Grb7 appears predominantly to be involved in focal adhesion kinase mediated cell migration
Huang et al., J Biol Chem 2010 : In addition to inhibiting insulin receptor and IGF1R kinase activity by directly binding to the receptors, GRB10 can also negatively regulate insulin and IGF1 signaling by mediating insulin receptor and IGF1R degradation through ubiquitination