◀ Back to PLCG1

LAT — PLCG1

Pathways - manually collected, often from reviews:

• KEGG Natural killer cell mediated cytotoxicity: LAT → PLCG1/PLCG2 (protein-protein, activation)
• KEGG T cell receptor signaling pathway: PLCG1 → LAT (protein-protein, binding/association)
• NCI Pathway Database TCR signaling in naïve CD8+ T cells: None → LAT/GRAP2/SLP76/VAV1/PLCgamma1 complex (LAT-GRAP2-LCP2-VAV1-PLCG1) (modification, collaborate)
  
• NCI Pathway Database TCR signaling in naïve CD8+ T cells: LAT/GRAP2/SLP76/VAV1/PLCgamma1 complex (LAT-GRAP2-LCP2-VAV1-PLCG1) → None (modification, activates)
• NCI Pathway Database Fc-epsilon receptor I signaling in mast cells: None → LAT/PLCgamma1/GRB2/SLP76/GADs complex (LAT-PLCG1-GRB2-LCP2) (modification, collaborate)
  Kettner et al., Mol Cell Biol 2003
  Evidence: assay, other species
• NCI Pathway Database Fc-epsilon receptor I signaling in mast cells: NTAL/PLCgamma1/GRB2/SLP76/GADs complex (LAT2-PLCG1-GRB2-LCP2) → LAT/PLCgamma1/GRB2/SLP76/GADs complex (LAT-PLCG1-GRB2-LCP2) (modification, activates)
  Kettner et al., Mol Cell Biol 2003
  Evidence: assay, other species
• NCI Pathway Database Fc-epsilon receptor I signaling in mast cells: LAT/PLCgamma1/GRB2/SLP76/GADs complex (LAT-PLCG1-GRB2-LCP2) → PLCgamma1 (PLCG1) (modification, activates) Kettner et al., Mol Cell Biol 2003
  Evidence: assay, other species
• NCI Pathway Database Fc-epsilon receptor I signaling in mast cells: LAT/PLCgamma1/GRB2/SLP76/GADs complex (LAT-PLCG1-GRB2-LCP2) (modification, activates) Kettner et al., Mol Cell Biol 2003
  Evidence: assay, other species
• NCI Pathway Database TCR signaling in naïve CD4+ T cells: CBL (CBL) → LAT/GRAP2/SLP76/VAV1/ITK/PLCgamma1/CBL complex (LAT-GRAP2-LCP2-VAV1-ITK-PLCG1) (modification, collaborate)
  Rellahan et al., Exp Cell Res 2003
  Evidence: physical interaction, other species
• NCI Pathway Database TCR signaling in naïve CD4+ T cells: CBL (CBL) → LAT/GRAP2/SLP76/VAV1/ITK/PLCgamma1 complex (LAT-GRAP2-LCP2-VAV1-ITK-PLCG1) (modification, collaborate)
  Rellahan et al., Exp Cell Res 2003
  Evidence: physical interaction, other species
• NCI Pathway Database TCR signaling in naïve CD4+ T cells: LAT/GRAP2/SLP76/VAV1/ITK/PLCgamma1/CBL complex (LAT-GRAP2-LCP2-VAV1-ITK-PLCG1) → LAT/GRAP2/SLP76/VAV1/ITK/PLCgamma1 complex (LAT-GRAP2-LCP2-VAV1-ITK-PLCG1) (modification, collaborate)
  Rellahan et al., Exp Cell Res 2003
  Evidence: physical interaction, other species
• NCI Pathway Database TCR signaling in naïve CD4+ T cells: PLCgamma1 (PLCG1) → LAT/GRAP2/SLP76/VAV1/ITK complex (LAT-GRAP2-LCP2-VAV1-ITK) (modification, collaborate)
  Zhang et al., J Biol Chem 2000, Yablonski et al., Mol Cell Biol 2001, Serrano et al., J Immunol 2005, Zhang et al., Cell 1998, Yablonski et al., Science 1998
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database TCR signaling in naïve CD4+ T cells: PLCgamma1 (PLCG1) → LAT/GRAP2/SLP76/VAV1/ITK/PLCgamma1 complex (LAT-GRAP2-LCP2-VAV1-ITK-PLCG1) (modification, collaborate)
  Zhang et al., J Biol Chem 2000, Yablonski et al., Mol Cell Biol 2001, Serrano et al., J Immunol 2005, Zhang et al., Cell 1998, Yablonski et al., Science 1998
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database TCR signaling in naïve CD4+ T cells: LAT/GRAP2/SLP76/VAV1/ITK complex (LAT-GRAP2-LCP2-VAV1-ITK) → LAT/GRAP2/SLP76/VAV1/ITK/PLCgamma1 complex (LAT-GRAP2-LCP2-VAV1-ITK-PLCG1) (modification, collaborate)
  Zhang et al., J Biol Chem 2000, Yablonski et al., Mol Cell Biol 2001, Serrano et al., J Immunol 2005, Zhang et al., Cell 1998, Yablonski et al., Science 1998
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database TCR signaling in naïve CD8+ T cells: PLCgamma1 (PLCG1) → LAT/GRAP2/SLP76/VAV1 complex (LAT-GRAP2-LCP2-VAV1) (modification, collaborate)
  Reynolds et al., J Exp Med 2002
  Evidence: mutant phenotype, other species
• NCI Pathway Database TCR signaling in naïve CD8+ T cells: PLCgamma1 (PLCG1) → LAT/GRAP2/SLP76/VAV1/PLCgamma1 complex (LAT-GRAP2-LCP2-VAV1-PLCG1) (modification, collaborate)
  Reynolds et al., J Exp Med 2002
  Evidence: mutant phenotype, other species
• NCI Pathway Database TCR signaling in naïve CD8+ T cells: LAT/GRAP2/SLP76/VAV1 complex (LAT-GRAP2-LCP2-VAV1) → LAT/GRAP2/SLP76/VAV1/PLCgamma1 complex (LAT-GRAP2-LCP2-VAV1-PLCG1) (modification, collaborate)
  Reynolds et al., J Exp Med 2002
  Evidence: mutant phenotype, other species
• NCI Pathway Database Class I PI3K signaling events: None → Src family/SYK family/BLNK-LAT/BTK-ITK/PLC-gamma complex (FGR_LYN_LCK_YES1_BLK_HCK_SRC_FYN-ZAP70_SYK-BLNK_LAT-ITK_BTK-PLCG1_PLCG2) (modification, collaborate)
  Rodriguez et al., Biochem J 2003
  Evidence: mutant phenotype, other species
• NCI Pathway Database Class I PI3K signaling events: Src family/SYK family/BLNK-LAT/BTK-ITK/PLC-gamma complex (FGR_LYN_LCK_YES1_BLK_HCK_SRC_FYN-ZAP70_SYK-BLNK_LAT-ITK_BTK-PLCG1_PLCG2) (modification, activates) Rodriguez et al., Biochem J 2003
  Evidence: mutant phenotype, other species
• NCI Pathway Database Class I PI3K signaling events: Src family/SYK family/BLNK-LAT/BTK-ITK/PLC-gamma complex (FGR_LYN_LCK_YES1_BLK_HCK_SRC_FYN-ZAP70_SYK-BLNK_LAT-ITK_BTK-PLCG1_PLCG2) → None (modification, activates) Rodriguez et al., Biochem J 2003
  Evidence: mutant phenotype, other species
• NCI Pathway Database Class I PI3K signaling events: PLC-gamma family (PLCG1/PLCG2) → Src family/SYK family/BLNK-LAT/BTK-ITK/PLC-gamma complex (FGR_LYN_LCK_YES1_BLK_HCK_SRC_FYN-ZAP70_SYK-BLNK_LAT-ITK_BTK-PLCG1_PLCG2) (modification, collaborate)
  Perez-Villar et al., J Immunol 1999, Rodriguez et al., Biochem J 2003, Serrano et al., J Immunol 2005
  Evidence: mutant phenotype, physical interaction, other species
• NCI Pathway Database Class I PI3K signaling events: PLC-gamma family (PLCG1/PLCG2) → Src family/SYK family/BLNK-LAT/BTK-ITK complex (FGR_LYN_LCK_YES1_BLK_HCK_SRC_FYN-ZAP70_SYK-BLNK_LAT-ITK_BTK) (modification, collaborate)
  Perez-Villar et al., J Immunol 1999, Rodriguez et al., Biochem J 2003, Serrano et al., J Immunol 2005
  Evidence: mutant phenotype, physical interaction, other species
• NCI Pathway Database Class I PI3K signaling events: Src family/SYK family/BLNK-LAT/BTK-ITK/PLC-gamma complex (FGR_LYN_LCK_YES1_BLK_HCK_SRC_FYN-ZAP70_SYK-BLNK_LAT-ITK_BTK-PLCG1_PLCG2) → Src family/SYK family/BLNK-LAT/BTK-ITK complex (FGR_LYN_LCK_YES1_BLK_HCK_SRC_FYN-ZAP70_SYK-BLNK_LAT-ITK_BTK) (modification, collaborate)
  Perez-Villar et al., J Immunol 1999, Rodriguez et al., Biochem J 2003, Serrano et al., J Immunol 2005
  Evidence: mutant phenotype, physical interaction, other species
• NCI Pathway Database Fc-epsilon receptor I signaling in mast cells: Antigen/IgE/Fc epsilon R1 complex (IGHE-MS4A2-FCER1G-FCER1A) → LAT/PLCgamma1/GRB2/SLP76/GADs complex (LAT-PLCG1-GRB2-LCP2) (modification, activates)
  Zhang et al., J Biol Chem 2000, Saitoh et al., J Exp Med 2003
• NCI Pathway Database Fc-epsilon receptor I signaling in mast cells: GRB2 (GRB2) → LAT/PLCgamma1/GRB2/SLP76/GADs complex (LAT-PLCG1-GRB2-LCP2) (modification, collaborate)
  Zhang et al., J Biol Chem 2000, Saitoh et al., J Exp Med 2003
• NCI Pathway Database Fc-epsilon receptor I signaling in mast cells: SLP76 (LCP2) → LAT/PLCgamma1/GRB2/SLP76/GADs complex (LAT-PLCG1-GRB2-LCP2) (modification, collaborate)
  Zhang et al., J Biol Chem 2000, Saitoh et al., J Exp Med 2003
• NCI Pathway Database Fc-epsilon receptor I signaling in mast cells: LAT/PLCgamma1/GRB2/SLP76/GADs complex (LAT-PLCG1-GRB2-LCP2) → LAT (LAT) (modification, collaborate) Zhang et al., J Biol Chem 2000, Saitoh et al., J Exp Med 2003
• NCI Pathway Database Fc-epsilon receptor I signaling in mast cells: LAT/PLCgamma1/GRB2/SLP76/GADs complex (LAT-PLCG1-GRB2-LCP2) → PLCgamma1 (PLCG1) (modification, collaborate) Zhang et al., J Biol Chem 2000, Saitoh et al., J Exp Med 2003
• NCI Pathway Database Fc-epsilon receptor I signaling in mast cells: LAT (LAT) → PLCgamma1 (PLCG1) (modification, collaborate) Zhang et al., J Biol Chem 2000, Saitoh et al., J Exp Med 2003
• NCI Pathway Database TCR signaling in naïve CD4+ T cells: None → LAT/GRAP2/SLP76/VAV1/ITK/PLCgamma1/CBL complex (LAT-GRAP2-LCP2-VAV1-ITK-PLCG1) (modification, collaborate)
  Graham et al., FEBS Lett 2000
  Evidence: mutant phenotype, assay
• NCI Pathway Database TCR signaling in naïve CD4+ T cells: None → LAT/GRAP2/SLP76/VAV1/ITK/PLCgamma1 complex (LAT-GRAP2-LCP2-VAV1-ITK-PLCG1) (modification, collaborate)
  Graham et al., FEBS Lett 2000
  Evidence: mutant phenotype, assay
• NCI Pathway Database TCR signaling in naïve CD4+ T cells: LAT/GRAP2/SLP76/VAV1/ITK/PLCgamma1/CBL complex (LAT-GRAP2-LCP2-VAV1-ITK-PLCG1) (modification, inhibits) Graham et al., FEBS Lett 2000
  Evidence: mutant phenotype, assay
• NCI Pathway Database TCR signaling in naïve CD4+ T cells: LAT/GRAP2/SLP76/VAV1/ITK/PLCgamma1/CBL complex (LAT-GRAP2-LCP2-VAV1-ITK-PLCG1) → LAT/GRAP2/SLP76/VAV1/ITK/PLCgamma1 complex (LAT-GRAP2-LCP2-VAV1-ITK-PLCG1) (modification, inhibits)
  Graham et al., FEBS Lett 2000
  Evidence: mutant phenotype, assay
• Reactome Reaction: LAT → PLCG1 (direct_complex) Kim et al., Exp Mol Med 2000
• Reactome Reaction: LAT → PLCG1 (reaction) Kim et al., Exp Mol Med 2000, Yablonski et al., Mol Cell Biol 2001
• Reactome Reaction: PLCG1 → LAT (reaction) Zhang et al., J Biol Chem 2000, Kim et al., Exp Mol Med 2000, Chong et al., J Biol Chem 2001, Yablonski et al., Mol Cell Biol 2001, Parrini et al., Mol Cell 2002, Wen et al., J Immunol 2002, Nore et al., Biochim Biophys Acta 2003, Tybulewicz et al., Immunol Rev 2003, Wang et al., Traffic 2003, Humphries et al., J Biol Chem 2004, Bos et al., Cell 2007, Peng et al., Science signaling 2010, Ormsby et al., Blood 2011, Todderud et al., Science 1990, Kawakami et al., Mol Cell Biol 1994, Rawlings et al., Science 1996, Park et al., Immunity 1996
• Reactome Reaction: PLCG1 → LAT (indirect_complex) Gross et al., J Biol Chem 1999, Zhang et al., J Biol Chem 2000, Yablonski et al., Mol Cell Biol 2001, Wen et al., J Immunol 2002, Reeve et al., J Immunol 2009, Peng et al., Science signaling 2010, Bubeck Wardenburg et al., J Biol Chem 1996, Hendricks-Taylor et al., J Biol Chem 1997
• Reactome Reaction: LAT → PLCG1 (indirect_complex) Kim et al., Exp Mol Med 2000, Yablonski et al., Mol Cell Biol 2001
• WikiPathways T-Cell antigen Receptor (TCR) pathway during Staphylococcus aureus infection: LAT → PLCG1 (activation)

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

• IRef Bind Interaction: PLCG1 — LAT Gilliland et al., J Biol Chem 1992*, Sieh et al., Mol Cell Biol 1994*, Zhang et al., Cell 1998
• IRef Bind_translation Interaction: PLCG1 — LAT (coimmunoprecipitation) Gilliland et al., J Biol Chem 1992*, Zhang et al., Cell 1998
• IRef Bind_translation Interaction: PLCG1 — LAT (experimental interaction detection) Gilliland et al., J Biol Chem 1992*
• IRef Bind_translation Interaction: PLCG1 — LAT (affinity chromatography technology) Gilliland et al., J Biol Chem 1992*
• IRef Bind_translation Interaction: PLCG1 — LAT (coimmunoprecipitation) Gilliland et al., J Biol Chem 1992*
• IRef Bind_translation Interaction: LAT — PLCG1 (coimmunoprecipitation) Gomez et al., Nat Immunol 2005
• IRef Biogrid Interaction: LAT — PLCG1 (colocalization, biochemical) Zhang et al., Immunity 1998*
• IRef Biogrid Interaction: LAT — PLCG1 (physical association, affinity chromatography technology) Paz et al., Biochem J 2001
• IRef Biogrid Interaction: LAT — PLCG1 (direct interaction, pull down) Paz et al., Biochem J 2001
• IRef Biogrid Interaction: LAT — PLCG1 (physical association, affinity chromatography technology) Zhang et al., Cell 1998
• IRef Biogrid Interaction: LAT — PLCG1 (physical association, affinity chromatography technology) Jiang et al., Leuk Res 2007
• IRef Biogrid Interaction: LAT — PLCG1 (physical association, affinity chromatography technology) Braiman et al., EMBO J 2006*
• IRef Biogrid Interaction: LAT — PLCG1 (direct interaction, fluorescent resonance energy transfer) Braiman et al., EMBO J 2006*
• MIPS CORUM LAT-PLC-gamma-1-p85-GRB2-CBL-VAV-SLP-76 signaling complex, C305 activated: LAT-PLC-gamma-1-p85-GRB2-CBL-VAV-SLP-76 signaling complex, C305 activated complex (CBL-GRB2-LAT-LCP2-PIK3R1-PLCG1-VAV1) Zhang et al., Cell 1998
• MIPS CORUM PLC-gamma-1-SLP-76-SOS1-LAT complex: PLC-gamma-1-SLP-76-SOS1-LAT complex complex (LAT-LCP2-PLCG1-SOS1) Gomez et al., Nat Immunol 2005
• MIPS CORUM LAT-PLC-gamma-1-p85-GRB2-SOS signaling complex, C305 activated: LAT-PLC-gamma-1-p85-GRB2-SOS signaling complex, C305 activated complex (GRB2-LAT-PIK3R1-PLCG1-SOS1) Zhang et al., Cell 1998
• MIPS CORUM LCK-SLP76-PLC-gamma-1-LAT complex, pervanadate-activated: LCK-SLP76-PLC-gamma-1-LAT complex, pervanadate-activated complex (LAT-LCK-LCP2-PLCG1) Jiang et al., Leuk Res 2007
• MIPS CORUM PLC-gamma-1-LAT-c-CBL complex, OKT3 stimulated: PLC-gamma-1-LAT-c-CBL complex, OKT3 stimulated complex (CBL-LAT-PLCG1) Jiang et al., Leuk Res 2007
• IRef Corum Interaction: Complex of LCK-LAT-PLCG1-LCP2 (association, coimmunoprecipitation) Jiang et al., Leuk Res 2007
• IRef Corum Interaction: Complex of LAT-PLCG1-PIK3R1-GRB2-SOS1 (association, coimmunoprecipitation) Zhang et al., Cell 1998
• IRef Corum Interaction: Complex of LAT-PLCG1-SOS1-LCP2 (association, coimmunoprecipitation) Gomez et al., Nat Immunol 2005
• IRef Corum Interaction: Complex of LCP2-GRB2-PIK3R1-CBL-LAT-PLCG1-VAV1 (association, coimmunoprecipitation) Zhang et al., Cell 1998
• IRef Corum Interaction: Complex of LAT-PLCG1-CBL (association, coimmunoprecipitation) Jiang et al., Leuk Res 2007
• IRef Hprd Interaction: LAT — PLCG1 (in vivo) Paz et al., Biochem J 2001, Braiman et al., EMBO J 2006*, Zhang et al., Immunity 1998*
• IRef Hprd Interaction: LAT — PLCG1 (in vitro) Paz et al., Biochem J 2001, Braiman et al., EMBO J 2006*, Zhang et al., Immunity 1998*
• IRef Hprd Interaction: Complex of 26 proteins (in vivo) Zhang et al., Cell 1998
• IRef Intact Interaction: LAT — PLCG1 (association, coimmunoprecipitation) Zhang et al., J Biol Chem 2000
• IRef Intact Interaction: LAT — PLCG1 (association, pull down) Zhang et al., J Biol Chem 2000
• IRef Intact Interaction: LAT — PLCG1 (physical association, anti bait coimmunoprecipitation) Jiang et al., Leuk Res 2007
• IRef Intact Interaction: LAT — PLCG1 (association, coimmunoprecipitation) Zhang et al., Cell 1998
• IRef Intact Interaction: Complex of LCK-PLCG1-LAT (association, anti bait coimmunoprecipitation) Jiang et al., Leuk Res 2007
• IRef Ophid Interaction: LAT — PLCG1 (aggregation, confirmational text mining) Paz et al., Biochem J 2001
• IRef Ophid Interaction: LAT — PLCG1 (aggregation, interologs mapping) Brown et al., Bioinformatics 2005

Text-mined interactions from Literome

Jevremovic et al., J Immunol 1999 : In addition to the biochemical evidence showing LAT involvement in NK cell activation, a genetic model shows that LAT is required for FcR dependent phosphorylation of phospholipase C-gamma
Zhang et al., Int Immunol 1999 : Using this cell line, we demonstrate that LAT is required for TCR mediated Ca2+ mobilization and optimal tyrosine phosphorylation of phospholipase C-gamma1 , Vav and SLP-76
Martelli et al., Blood 2000 : Using Jurkat cell lines deficient in ZAP70/Syk ( P116 ) or LAT ( ANJ3 ) expression, CD2 dependent PLCgamma-1 and SLP-76 tyrosine phosphorylation required expression both of ZAP70 or Syk and of LAT
Graham et al., Oncogene 2003 : 70Z/3 Cbl induced PLC gamma 1 phosphorylation required Zap-70, as for the TCR, and the tyrosine kinase binding domain of 70Z/3 Cbl, which binds Zap-70, but did not require PLC gamma 1 binding to Lat , a crucial interaction in TCR induced PLC gamma 1 phosphorylation
Finco et al., Immunity 1998 : LAT is required for TCR mediated activation of PLCgamma1 and the Ras pathway