◀ Back to SMAD3
JUN — SMAD3
Pathways - manually collected, often from reviews:
-
FastForward regulation:
SMAD3
→
JUN
(transcriptional regulation, unknown)
Wong et al., Mol Cell Biol 1999*
Evidence: DNABINDING
-
NCI Pathway Database Regulation of nuclear SMAD2/3 signaling:
AP1 complex (FOS-JUN)
→
SMAD2-3/SMAD4/SP1 complex (SMAD2_SMAD3-SMAD4-SP1)
(transcription, activates)
Brodin et al., J Biol Chem 2000
Evidence: mutant phenotype, reporter gene, physical interaction, other species
-
NCI Pathway Database Regulation of nuclear SMAD2/3 signaling:
SMAD3/SMAD4/JUN/FOS complex (SMAD3-SMAD4-FOS-JUN)
→
JUN (JUN)
(transcription, activates)
Wong et al., Mol Cell Biol 1999*, Inman et al., J Biol Chem 2002*
Evidence: mutant phenotype, reporter gene, physical interaction
-
NCI Pathway Database Regulation of nuclear SMAD2/3 signaling:
AP1 complex (FOS-JUN)
→
SMAD3/SMAD4 complex (SMAD3-SMAD4)
(modification, collaborate)
Zhang et al., Nature 1998*
Evidence: physical interaction
-
NCI Pathway Database Regulation of nuclear SMAD2/3 signaling:
AP1 complex (FOS-JUN)
→
SMAD3/SMAD4/JUN/FOS complex (SMAD3-SMAD4-FOS-JUN)
(modification, collaborate)
Zhang et al., Nature 1998*
Evidence: physical interaction
-
NCI Pathway Database Regulation of nuclear SMAD2/3 signaling:
SMAD3/SMAD4 complex (SMAD3-SMAD4)
→
SMAD3/SMAD4/JUN/FOS complex (SMAD3-SMAD4-FOS-JUN)
(modification, collaborate)
Zhang et al., Nature 1998*
Evidence: physical interaction
Protein-Protein interactions - manually collected from original source literature:
Studies that report less than 10 interactions are marked with *
-
IRef Biogrid Interaction:
SMAD3
—
JUN
(physical association, affinity chromatography technology)
Lee et al., PloS one 2011*
-
IRef Biogrid Interaction:
SMAD3
—
JUN
(direct interaction, pull down)
Liberati et al., J Biol Chem 2001*
-
IRef Biogrid Interaction:
SMAD3
—
JUN
(direct interaction, two hybrid)
Zhang et al., Nature 1998*
-
IRef Biogrid Interaction:
SMAD3
—
JUN
(physical association, affinity chromatography technology)
Verrecchia et al., J Biol Chem 2000*
-
IRef Biogrid Interaction:
SMAD3
—
JUN
(physical association, affinity chromatography technology)
Zhang et al., Nature 1998*
-
IRef Biogrid Interaction:
SMAD3
—
JUN
(direct interaction, pull down)
Zhang et al., Nature 1998*
-
IRef Biogrid Interaction:
SMAD3
—
JUN
(direct interaction, pull down)
Liberati et al., Proc Natl Acad Sci U S A 1999
-
MIPS CORUM SMAD3-SMAD4-cJun-cFos complex:
SMAD3-SMAD4-cJun-cFos complex complex (FOS-JUN-SMAD3-SMAD4)
Zhang et al., Nature 1998*
-
MIPS CORUM SMAD3-SMAD4-cJUN complex:
SMAD3-SMAD4-cJUN complex complex (JUN-SMAD3-SMAD4)
Liberati et al., Proc Natl Acad Sci U S A 1999
-
IRef Corum Interaction:
Complex of SMAD4-SMAD3-JUN
(association, pull down)
Liberati et al., Proc Natl Acad Sci U S A 1999
-
IRef Corum Interaction:
Complex of SMAD4-FOS-SMAD3-JUN
(association, electrophoretic mobility supershift assay)
Zhang et al., Nature 1998*
-
IRef Hprd Interaction:
SMAD3
—
JUN
(in vitro)
Zhang et al., Nature 1998*
-
IRef Hprd Interaction:
SMAD3
—
JUN
(in vivo)
Zhang et al., Nature 1998*
-
IRef Hprd Interaction:
Complex of 17 proteins
(in vivo)
Zhang et al., Nature 1998*
-
IRef Ophid Interaction:
SMAD3
—
JUN
(aggregation, confirmational text mining)
Zhang et al., Nature 1998*
-
IRef Ophid Interaction:
SMAD3
—
JUN
(aggregation, interologs mapping)
Brown et al., Bioinformatics 2005
Text-mined interactions from Literome
Dennler et al., J Biol Chem 2000
:
However,
c-Jun does not
inhibit Smad3 binding to DNA in vitro
Qing et al., J Biol Chem 2000
:
Mutational analyses of the c-Jun protein and the AP-1 binding site in the promoter revealed that the interaction of
c-Jun with DNA is
necessary for transcriptional activation by TGF-beta and
Smad3
Verrecchia et al., Oncogene 2001
:
Finally, we have determined that off-DNA interactions between Smad3 and both
c-Jun and JunB
result in the reduction of
Smad3/DNA interactions
Feinberg et al., Circ Res 2004
(Hyperplasia...) :
We demonstrate that the inhibitory effect of Smad3 occurs via a novel antagonistic
effect of
Smad3 on
AP-1 DNA-protein binding and activity
Zhang et al., Am J Pathol 2006
:
Furthermore,
Smad3 blockade specifically
inhibited activation of the transcription factor
AP-1 by TGF-beta1, whereas PD98059 prevented TGF-beta1 dependent nuclear factor-kappaB activation
Sundqvist et al., Oncogene 2013
(Breast Neoplasms...) :
Finally, chromatin immunoprecipitations showed that
c-Jun , JunB and Fra1, but not c-Fos, are
required for TGFß induced binding of
Smad2/3 to the mmp-10 and pai-1 promoters
Mucsi et al., Biochem Biophys Res Commun 1997
:
Activation of the PAI-1 promoter by TGF-beta or prostaglandin F2 alpha, and transactivation by
c-Jun or JunB were not
inhibited by dominant negative
Smad3 , supporting the specificity of this mutant