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EPHB2 — PTH

Text-mined interactions from Literome

Sneddon et al., Endocrinology 2000 : We examined PTH activation of the mitogen activated protein kinase ( MAPK ) cascade raf-MEK-ERK in PCT and DCT cells and its effects on calcium transport and signaling ... In DCT cells, PTH stimulates phosphorylation of ERK2 and activation of ERK2 kinase and is blocked by the MEK inhibitor PD98059 ... In DCT cells, stimulation of calcium entry with ionomycin did not activate ERK2 or augment PTH stimulated ERK2 activity, indicating that MAPK activation lies upstream of calcium entry ... ERK2 activation by PTH was blocked by the protein kinase C inhibitor calphostin-C but was unaffected by the protein kinase A inhibitor Rp-cAMPs ... PTH also induced ERK2 phosphorylation in PCT cells
Ahmed et al., Mol Endocrinol 2003 : In TMOb cells, stimulation of endogenous Gs/Gq coupled PTH receptors, Gq-coupled PGF2 alpha receptors, and Gi/Gq coupled lysophosphatidic acid receptors, but not Gs-coupled PGE2 receptors, caused a rapid 5- to 10-fold increase in ERK1/2 phosphorylation ... When PTH receptor expressing COS-7 cells were placed in a mixed culture with cells lacking the PTH receptor but expressing a green fluorescent protein tagged ERK2, stimulation with PTH ( 1-34 ) induced phosphorylation of green fluorescent protein-ERK2 that was abolished by either batimastat or tyrphostin AG1478
Sakwe et al., Exp Cell Res 2004 : Activation of ERK1/2 by high [ Ca ( 2+ ) ] ( o ) was not necessary for the [ Ca ( 2+ ) ] ( o ) -regulated secretion of parathyroid hormone (PTH) in dispersed bovine parathyroid cells
Gentili et al., J Cell Biochem 2004 : Collectively, these data suggest that PTH activates in duodenal cells, a Ca2+ dependent cytosolic PLA2 and attendant AA release and that this activation requires prior stimulation of intracellular ERK1/2 , PKA, and PKC
Syme et al., J Biol Chem 2005 : PTH- ( 1-34 ) induced rapid and robust activation of ERK1/2
Khundmiri et al., J Biol Chem 2005 (MAP Kinase Signaling System) : In contrast, PTH stimulated ERK activation was not inhibited by pretreatment with the PKCalpha inhibitory peptide ... Inhibition of PKCalpha expression by siRNA did not inhibit PTH mediated ERK activation but significantly reduced PTH mediated phosphorylation of the Na+-K+-ATPase alpha1 subunit ... Pharmacologic inhibition of phosphoinositide 3-kinase blocked PTH stimulated ERK activation, translocation of PKCalpha, and phosphorylation of the Na+-K+-ATPase alpha1 subunit
Saito et al., Biochem J 2005 : PTH- ( 1-34 ) caused phosphorylation of ERK ( extracellular-signal regulated kinase ) 1/2 in PTHR expressed cells mainly mediated through EGF ( epidermal growth factor) receptor
Kim et al., Biochem Biophys Res Commun 2006 : This PTH stimulated ERK1/2 activation was inhibited by cyclosporine A and FK506
Gesty-Palmer et al., J Biol Chem 2006 (MAP Kinase Signaling System) : The purpose of the present study was to identify the contributions of distinct signaling mechanisms to PTH stimulated activation of the mitogen activated protein kinases ( MAPK) ERK1/2 ... However, these inhibitors of second messenger dependent kinases had little effect on the later phase of PTH stimulated ERK1/2 phosphorylation ... The time course of ERK1/2 activation was biphasic with an early peak at 10 min and a later sustained ERK1/2 activation persisting for greater than 60 min. Pretreatment of HEK293 cells with the PKA inhibitor H89 or the PKC inhibitor GF109203X, individually or in combination reduced the early component of PTH stimulated ERK activity ... It is concluded that PTH stimulates ERK1/2 through several distinct signal transduction pathways : an early G protein dependent pathway meditated by PKA and PKC and a late pathway independent of G proteins mediated through beta-arrestins
Sneddon et al., American journal of physiology. Renal physiology 2007 : The role of epidermal growth factor receptor (EGFR) transactivation in ERK1/2 activation by PTH in distal kidney cells, a primary site of PTH action, was characterized ... ERK1/2 phosphorylation was stimulated by PTH and blocked by the EGFR inhibitor, AG1478 ... PTH stimulated ERK by wild-type, mutated and truncated PTH1Rs 21-, 27- and 57-fold, respectively ... Pertussis toxin inhibited PTH stimulated ERK1/2 by mutated and truncated PTH1Rs and abolished ERK1/2 activation by wild-type PTH1R
Sneddon et al., Endocrinology 2007 : We characterized PTH stimulated ERK activation and the involvement of receptor internalization and betaAr dependence ... In DCT cells, PTH transiently activated ERK maximally at 5 min and then returned to baseline ... In MEFs null for betaAr1 and -2, PTH stimulated ERK activation peaked by 5 min and returned to baseline ... PTH stimulated ERK activation and receptor endocytosis were not inhibited by the clathrin binding domain of betaAr1 [ Ar ( 319-418 ) ] ... We conclude that PTH stimulated ERK activation in DCT cells proceeds with a rapid but transient phase that may involve betaAr1
Somjen et al., Bone 2008 : Finally, PTH- induced ERK1/2 phosphorylation, was blocked by a MEK inhibitor
Khundmiri et al., American journal of physiology. Renal physiology 2008 : PTH mediated regulation of Na+-K+-ATPase requires Src kinase dependent ERK phosphorylation ... In the present work utilizing opossum kidney cells, a model of renal proximal tubule, PTH stimulated ERK phosphorylation and membrane translocation of PKCalpha were prevented by inhibition of Src kinase, PLC, and calcium entry ... Pharmacological inhibition of PLA2 did not prevent PTH stimulated ERK phosphorylation but completely prevented PKCalpha translocation
Lai et al., Cell Biochem Funct 2009 : We investigated the mechanisms by which PTH inhibits ERK1/2 activity in osteoblastic UMR 106-01 cells ... Our results suggest that rapid PTH inhibition of ERK1/2 activity is mediated by PKA dependent inhibition of c-Raf activity and that stimulation of MKP-1 may contribute to maintaining pERK1/2 inhibition over prolonged time
Pallais et al., J Clin Endocrinol Metab 2011 (Autoimmune Diseases...) : The anti-CaSR antibody mediated inhibition of CaSR stimulated ERK1/2 activity, but not of inositol-1-phosphate accumulation, suggests that ERK1/2 may mediate , at least in part, the regulation of PTH secretion and urinary calcium excretion by the CaSR
Yuan et al., J Bone Miner Res 2011 (Hypophosphatemia, Familial) : PTH treatment similarly induced cAMP accumulation and phosphorylation of ERK1/2 and CREB in the osteoblasts from each genotype
Tawfeek et al., Am J Physiol Endocrinol Metab 2012 (MAP Kinase Signaling System) : Importantly, expression of a receptor phosphorylation independent ß-arrestin2 ( R169E ) in the PD-PPR cells restored the biphasic effect of PTH on ERK1/2 as in the WT-PPR cells
Mahalingam et al., J Endocrinol 2013 : We conclude that PTH regulation of osteoblast mineralization in female mice is maturation stage specific and involves MKP1 modulation of P-ERK and P-p38 MAPKs