◀ Back to EPHB2
EFNB2 — EPHB2
Pathways - manually collected, often from reviews:
-
KEGG Axon guidance:
EPHB1/EPHB2/EPHB3/EPHB4/EPHB6
→
EFNB1/EFNB2/EFNB3
(protein-protein, activation)
-
NCI Pathway Database EPHB forward signaling:
EPHB2 (EPHB2)
→
Ephrin B (EFNB3/EFNB2/EFNB1)
(modification, collaborate)
Zisch et al., Oncogene 2000, Himanen et al., Nature 2001, Gale et al., Neuron 1996
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database EPHB forward signaling:
EPHB2 (EPHB2)
→
Ephrin B/EPHB2 complex (EFNB3_EFNB2_EFNB1-EPHB2)
(modification, collaborate)
Zisch et al., Oncogene 2000, Himanen et al., Nature 2001, Gale et al., Neuron 1996
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database EPHB forward signaling:
Ephrin B (EFNB3/EFNB2/EFNB1)
→
Ephrin B/EPHB2 complex (EFNB3_EFNB2_EFNB1-EPHB2)
(modification, collaborate)
Zisch et al., Oncogene 2000, Himanen et al., Nature 2001, Gale et al., Neuron 1996
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database EPHB forward signaling:
RasGAP (RASA1)
→
Ephrin B/EPHB2 complex (EFNB3_EFNB2_EFNB1-EPHB2)
(modification, collaborate)
Elowe et al., Mol Cell Biol 2001, Holland et al., EMBO J 1997
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database EPHB forward signaling:
RasGAP (RASA1)
→
Ephrin B/EPHB2/RasGAP complex (EFNB3_EFNB2_EFNB1-EPHB2-RASA1)
(modification, collaborate)
Elowe et al., Mol Cell Biol 2001, Holland et al., EMBO J 1997
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database EPHB forward signaling:
Ephrin B/EPHB2 complex (EFNB3_EFNB2_EFNB1-EPHB2)
→
Ephrin B/EPHB2/RasGAP complex (EFNB3_EFNB2_EFNB1-EPHB2-RASA1)
(modification, collaborate)
Elowe et al., Mol Cell Biol 2001, Holland et al., EMBO J 1997
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database EPHB forward signaling:
None
→
Ephrin B/EPHB2/Intersectin/N-WASP complex (EFNB3_EFNB2_EFNB1-EPHB2-ITSN1-WASL)
(modification, collaborate)
Irie et al., Nat Neurosci 2002
Evidence: mutant phenotype, assay
-
NCI Pathway Database EPHB forward signaling:
CDC42/GDP complex (CDC42)
→
Ephrin B/EPHB2/Intersectin/N-WASP complex (EFNB3_EFNB2_EFNB1-EPHB2-ITSN1-WASL)
(modification, collaborate)
Irie et al., Nat Neurosci 2002
Evidence: mutant phenotype, assay
-
NCI Pathway Database Ephrin B reverse signaling:
Ephrin B2 (EFNB2)
→
EPHB1-2 (EPHB1/EPHB2)
(modification, collaborate)
Davis et al., Science 1994, Gale et al., Neuron 1996
Evidence: assay, physical interaction
-
NCI Pathway Database Ephrin B reverse signaling:
Ephrin B2 (EFNB2)
→
Ephrin B2/EPHB1-2 complex (EFNB2-EPHB1_EPHB2)
(modification, collaborate)
Davis et al., Science 1994, Gale et al., Neuron 1996
Evidence: assay, physical interaction
-
NCI Pathway Database Ephrin B reverse signaling:
EPHB1-2 (EPHB1/EPHB2)
→
Ephrin B2/EPHB1-2 complex (EFNB2-EPHB1_EPHB2)
(modification, collaborate)
Davis et al., Science 1994, Gale et al., Neuron 1996
Evidence: assay, physical interaction
-
NCI Pathway Database EPHB forward signaling:
Endophilin/SYNJ1 complex (SYNJ1)
→
Ephrin B/EPHB2 complex (EFNB3_EFNB2_EFNB1-EPHB2)
(modification, collaborate)
Irie et al., Nat Cell Biol 2005*
Evidence: mutant phenotype, assay
-
NCI Pathway Database EPHB forward signaling:
Ephrin B2 (EFNB2)
→
EPHB1-2 (EPHB1/EPHB2)
(modification, collaborate)
Davis et al., Science 1994, Gale et al., Neuron 1996
Evidence: assay, physical interaction
-
NCI Pathway Database EPHB forward signaling:
Ephrin B2 (EFNB2)
→
Ephrin B2/EPHB1-2 complex (EFNB2-EPHB1_EPHB2)
(modification, collaborate)
Davis et al., Science 1994, Gale et al., Neuron 1996
Evidence: assay, physical interaction
-
NCI Pathway Database EPHB forward signaling:
EPHB1-2 (EPHB1/EPHB2)
→
Ephrin B2/EPHB1-2 complex (EFNB2-EPHB1_EPHB2)
(modification, collaborate)
Davis et al., Science 1994, Gale et al., Neuron 1996
Evidence: assay, physical interaction
-
NCI Pathway Database EPHB forward signaling:
Ephrin B/EPHB2/KALRN complex (EFNB3_EFNB2_EFNB1-EPHB2-KALRN)
→
KALRN (KALRN)
(modification, collaborate)
Penzes et al., Neuron 2003
Evidence: physical interaction, other species
-
NCI Pathway Database EPHB forward signaling:
Ephrin B/EPHB2/KALRN complex (EFNB3_EFNB2_EFNB1-EPHB2-KALRN)
→
Ephrin B/EPHB2 complex (EFNB3_EFNB2_EFNB1-EPHB2)
(modification, collaborate)
Penzes et al., Neuron 2003
Evidence: physical interaction, other species
-
NCI Pathway Database EPHB forward signaling:
KALRN (KALRN)
→
Ephrin B/EPHB2 complex (EFNB3_EFNB2_EFNB1-EPHB2)
(modification, collaborate)
Penzes et al., Neuron 2003
Evidence: physical interaction, other species
-
NCI Pathway Database EPHB forward signaling:
Ephrin B/EPHB2/RasGAP complex (EFNB3_EFNB2_EFNB1-EPHB2-RASA1)
→
RAS family/GDP complex (HRAS_KRAS_HRAS_KRAS_NRAS)
(modification, activates)
Elowe et al., Mol Cell Biol 2001
Evidence: genetic interaction
-
NCI Pathway Database EPHB forward signaling:
Ephrin B/EPHB2/RasGAP complex (EFNB3_EFNB2_EFNB1-EPHB2-RASA1)
→
None
(modification, activates)
Elowe et al., Mol Cell Biol 2001
Evidence: genetic interaction
-
NCI Pathway Database EPHB forward signaling:
Ephrin B/EPHB2/RasGAP complex (EFNB3_EFNB2_EFNB1-EPHB2-RASA1)
→
RAS family/GTP complex (HRAS_KRAS_HRAS_KRAS_NRAS)
(modification, activates)
Elowe et al., Mol Cell Biol 2001
Evidence: genetic interaction
-
NCI Pathway Database EPHB forward signaling:
Ephrin B/EPHB2/RasGAP complex (EFNB3_EFNB2_EFNB1-EPHB2-RASA1)
→
None
(modification, activates)
Elowe et al., Mol Cell Biol 2001
Evidence: genetic interaction
-
NCI Pathway Database EPHB forward signaling:
Ephrin B/EPHB2/KALRN complex (EFNB3_EFNB2_EFNB1-EPHB2-KALRN)
→
PAK1 (PAK1)
(modification, activates)
Penzes et al., Neuron 2003
Evidence: mutant phenotype, other species
-
NCI Pathway Database EPHB forward signaling:
Ephrin B/EPHB2/Intersectin/N-WASP complex (EFNB3_EFNB2_EFNB1-EPHB2-ITSN1-WASL)
→
Ephrin B/EPHB2 complex (EFNB3_EFNB2_EFNB1-EPHB2)
(modification, collaborate)
Irie et al., Nat Neurosci 2002
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database EPHB forward signaling:
Ephrin B/EPHB2/Intersectin/N-WASP complex (EFNB3_EFNB2_EFNB1-EPHB2-ITSN1-WASL)
→
Intersectin/N-WASP complex (ITSN1-WASL)
(modification, collaborate)
Irie et al., Nat Neurosci 2002
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database EPHB forward signaling:
Ephrin B/EPHB2 complex (EFNB3_EFNB2_EFNB1-EPHB2)
→
Intersectin/N-WASP complex (ITSN1-WASL)
(modification, collaborate)
Irie et al., Nat Neurosci 2002
Evidence: mutant phenotype, physical interaction
-
Reactome Reaction:
EFNB2
→
EPHB2
(reaction)
van Horck et al., J Biol Chem 2001, Marignani et al., J Cell Biol 2001, Himanen et al., Nature 2001, Himanen et al., Trends Neurosci 2003, Bokoch et al., Annu Rev Biochem 2003, Zhang et al., J Cell Biol 2003, Marston et al., Nat Cell Biol 2003, Zhang et al., J Neurosci 2005, Cowan et al., Neuron 2005, Tolias et al., Proc Natl Acad Sci U S A 2007, Himanen et al., Curr Opin Cell Biol 2007, Zhang et al., Nature 1990, Yao et al., Oncogene 1995
-
Reactome Reaction:
EFNB2
→
EPHB2
(direct_complex)
Himanen et al., Nature 2001, Himanen et al., Trends Neurosci 2003, Himanen et al., Curr Opin Cell Biol 2007
-
Reactome Reaction:
EFNB2
→
EPHB2
(indirect_complex)
Penzes et al., Neuron 2003
Protein-Protein interactions - manually collected from original source literature:
Studies that report less than 10 interactions are marked with *
-
IRef Bind Interaction:
EFNB2
—
EPHB2
Gale et al., Neuron 1996
-
IRef Bind_translation Interaction:
EFNB2
—
EPHB2
(experimental interaction detection)
Gale et al., Neuron 1996
-
IRef Biogrid Interaction:
EFNB2
—
EPHB2
(direct interaction, pull down)
Himanen et al., Nature 2001
-
IRef Hprd Interaction:
EFNB2
—
EPHB2
(in vitro)
Himanen et al., Nature 2001
-
IRef Hprd Interaction:
EFNB2
—
EPHB2
(in vivo)
Himanen et al., Nature 2001
-
IRef Ophid Interaction:
EFNB2
—
EPHB2
(aggregation, confirmational text mining)
Himanen et al., Nature 2001
-
IRef Ophid Interaction:
EFNB2
—
EPHB2
(aggregation, interologs mapping)
Brown et al., Bioinformatics 2005
Text-mined interactions from Literome
Takasu et al., Science 2002
:
EphrinB2 activation of
EphB in primary cortical neurons potentiates NMDA receptor dependent influx of calcium
Kim et al., FASEB J 2002
(MAP Kinase Signaling System) :
EphrinB2 not only
increased the phosphorylation of
EphB2 and EphB4 in a time dependent manner but also increased recruitment of p120-Ras-GTPase activating protein ( p120-RasGAP ) to EphB2 and EphB4
Mann et al., J Neurobiol 2003
:
Forward and reverse signaling
mediated by
EphB tyrosine kinase receptors and their transmembrane
ephrin-B ligands play important roles in axon pathfinding, yet little is known about the intracellular pathways involved
Henkemeyer et al., J Cell Biol 2003
:
We show further that a kinase-defective, truncating mutation in EphB2 also results in abnormal spine development and that
ephrin-B2 mediated activation of the
EphB receptors accelerates dendritic spine development
Georgakopoulos et al., EMBO J 2006
:
Here we show that
EphB stimulates a metalloproteinase cleavage of
ephrinB2 , producing a carboxy-terminal fragment that is further processed by PS1/gamma-secretase to produce intracellular peptide ephrinB2/CTF2 ... PS1 FAD and gamma-secretase dominant negative mutants inhibited the
EphB induced cleavage of
ephrinB2 and Src autophosphorylation, raising the possibility that FAD mutants interfere with the functions of Src and ephrinB2 in the CNS
Prestoz et al., Neuron Glia Biol 2004
:
In addition, we demonstrate that coated EphB2-Fc receptors, which are specific for ephrinB2/B3 ligands, induce dramatic changes in the contact and migratory properties of OPCs, indicating that axonal
EphB receptors
activate ephrinB signaling in OPCs.Based on these findings, we propose that OPCs are characterized by an ephrin code, and that Eph-ephrin interactions between axons and OPCs control the distribution of OPCs in the optic axonal tracts, and the progress and arrest of their migration
Lin et al., J Biol Chem 2008
:
We found that
EphB2 is cleaved by MMPs both in vitro and in vivo, and that this cleavage is
induced by interaction with its ligand
ephrin-B2
Bush et al., Genes Dev 2009
:
In addition to the ability of ephrin-B1 to forward signal through its cognate
EphB tyrosine kinase receptors,
ephrin-B1 can also
act as a receptor and transduce a reverse signal by either PDZ dependent or phosphorylation dependent mechanisms
Li et al., J Neurosci 2011
:
These data provide evidence for
ephrin-B induced
EphB2 forward signaling in presynaptic structural plasticity during classical conditioning
Georgakopoulos et al., FASEB J 2011
:
Presenilin1/gamma-secretase promotes the
EphB2 induced phosphorylation of
ephrinB2 by regulating phosphoprotein associated with glycosphingolipid enriched microdomains/Csk binding protein ...
EphB2 induced tyrosine phosphorylation of
ephrinB2 depends on PAG/Cbp because EphB2 can not increase ephrinB2 phosphorylation in cells treated with anti-PAG siRNA or in PAG/Cbp-knockout ( KO ) cells ... Furthermore, in contrast to WT PS1, familial Alzheimer disease ( FAD ) PS1 mutants expressed in PS1-KO mouse embryonic fibroblasts inhibited both the
EphB2 induced dephosphorylation of PAG/Cbp and the phosphorylation of
ephrinB2
Muñoz et al., Neuroimmunomodulation 2011
:
In addition,
EphB2 and EphB3 play a cell-autonomous role in regulating the transitions of double negative to double positive cells and of double positive to single positive thymocytes and the lack of these molecules or their ligands ephrin B1 and
ephrin B2 induces profound alterations of the TEC maturation and in the arrangement of epithelial network
Barthet et al., Neurobiol Aging 2013
:
Activation of
EphB receptors by
ephrinB ( efnB ) ligands on neuronal cell surface regulates important functions, including neurite outgrowth, axonal guidance, and synaptic plasticity
Srivastava et al., Mol Cell Neurosci 2013
:
Taken together, our findings suggest that
ephrinB activated
EphB2 receptors recruit a novel Nck/Pak signaling complex to mediate repulsive cortical growth cone guidance, which may be relevant for EphB forward signaling dependent axon guidance in vivo