Gene interactions and pathways from curated databases and text-mining

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CUL1 — SKP1

Pathways - manually collected, often from reviews:

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

Text-mined interactions from Literome

Hatakeyama et al., Proc Natl Acad Sci U S A 1999 : These results suggest that the substrate-specific degradation of IkappaBalpha is mediated by a Skp1/Cull 1/F-box protein (SCF) FWD1 ubiquitin-ligase complex and that FWD1 serves as an intracellular receptor for phosphorylated IkappaBalpha
Sui et al., Blood 1999 : The CD34 ( + ) IL-6R- cells produced a number of Mks as well as Mk colonies in cultures supplemented with sIL-6R/IL-6 or TPO in the presence of SCF
Huang et al., Blood 1999 : However, interleukin-5 (IL-5) alone sustained survival of TF-1 cells and required costimulation of SCF for optimal proliferation ... However, interleukin-5 (IL-5) alone sustained survival of TF-1 cells and required costimulation of SCF for optimal proliferation
Janowska-Wieczorek et al., Blood 1999 (Breast Neoplasms) : Moreover, the stimulatory effects of G-CSF and SCF on both MMP-2 and MMP-9 secretion were found to be significantly higher in CD34 ( + ) cells isolated from bone marrow than in those from peripheral blood ... Moreover, the stimulatory effects of G-CSF and SCF on both MMP-2 and MMP-9 secretion were found to be significantly higher in CD34 ( + ) cells isolated from bone marrow than in those from peripheral blood
Bao et al., Blood 1999 : We found that erythropoietin (EPO) and stem cell factor (SCF) activated protein kinase B ( PKB/Akt ) in EPO dependent HCD57 erythroid cells
Price et al., Biochem Biophys Res Commun 1999 : Taken together, these findings show that CHK is able to down-regulate SCF/KL stimulated Lyn activity in megakaryocytes
Tsvetkov et al., Curr Biol 1999 : p27 ( Kip1 ) ubiquitination and degradation is regulated by the SCF(Skp2) complex through phosphorylated Thr187 in p27
Mou et al., Biochem J 1999 : These findings demonstrate that SCF activates the Src family member Lyn before the G ( 1 ) /S transition of the cell cycle and suggest that Lyn is involved in SCF induced cell cycle progression
Rouillon et al., EMBO J 2000 : Feedback regulated degradation of the transcriptional activator Met4 is triggered by the SCF ( Met30 ) complex
Jiang et al., Blood 2000 : In contrast, cyclin-E and p27 ( KIP1 ) protein levels and cdk2 activity in cells adherent to FN in the presence of IL3 or SCF were similar to those in PLL-adherent and FN-nonadherent or PLL-nonadherent cells ... In contrast, cyclin-E and p27 ( KIP1 ) protein levels and cdk2 activity in cells adherent to FN in the presence of IL3 or SCF were similar to those in PLL-adherent and FN-nonadherent or PLL-nonadherent cells
Blume-Jensen et al., Nat Genet 2000 : The mutation completely disrupted PI 3'-kinase binding to Kit/SCF-R and reduced SCF induced PI 3'-kinase dependent activation of Akt by 90 %
Meimoun et al., Mol Biol Cell 2000 : Here we report that SCF ( CDC4 ), a recently characterized protein complex that acts in conjunction with the ubiquitin conjugating enzyme Cdc34 to degrade cell cycle regulators, is also necessary for the degradation of the transcription factor Gcn4. Degradation of Gcn4 occurs throughout the cell cycle, whereas degradation of the known cell cycle substrates of Cdc34/SCF(CDC4) is cell cycle regulated
Read et al., Mol Cell Biol 2000 : The site of Nedd8 ligation to Cul-1 is essential, as SCF(beta(TrCP)) containing a K720R mutant of Cul-1 only weakly supported IkappaBalpha ubiquitination compared to SCF(beta(TrCP)) containing WT Cul-1, suggesting that the Nedd8 ligation of Cul-1 affects the ubiquitination activity of SCF(beta(TrCP)) ... The site of Nedd8 ligation to Cul-1 is essential, as SCF(beta(TrCP)) containing a K720R mutant of Cul-1 only weakly supported IkappaBalpha ubiquitination compared to SCF(beta(TrCP)) containing WT Cul-1, suggesting that the Nedd8 ligation of Cul-1 affects the ubiquitination activity of SCF(beta(TrCP))
Feng et al., J Biol Chem 2000 : SCF induced cyclin D3 expression and phosphorylation of the retinoblastoma protein through a pathway that is sensitive to both wortmannin and rapamycin
Bondzi et al., Cell Growth Differ 2000 (Carcinoma, Small Cell...) : In the present study, we have demonstrated that PP1, a pharmacological inhibitor of Src kinases, blocked SCF mediated activation of mitogen activated protein ( MAP ) kinase , but it also inhibited Kit activation
Sui et al., Br J Haematol 2000 : We have recently demonstrated that SCF and EPO synergistically activate mitogen activated protein ( MAP ) kinase , thereby promoting growth of human erythroid colony forming cells ( ECFCs )
Krystal et al., Clin Cancer Res 2000 (Carcinoma, Small Cell...) : STI571 efficiently blocked SCF mediated activation of mitogen activated protein kinase and Akt, but did not affect insulin-like growth factor-1 or serum mediated mitogen activated protein kinase or Akt activation ... STI571 efficiently blocked SCF mediated activation of mitogen activated protein kinase and Akt , but did not affect insulin-like growth factor-1 or serum mediated mitogen activated protein kinase or Akt activation
Erickson-Miller et al., Stem Cells 2000 (Leukemia, Erythroblastic, Acute) : The activation of MAPK ( p42/44 ) by SCF was transient, lasting only 30 min, in contrast to Epo, which stimulated phosphorylation of p42/44 for up to 2 h ... The expression of the early response genes c-fos, egr1, and cytokine-inducible SH2 protein (CIS) in response to SCF or Epo stimulation demonstrated that the transient expression of p42/44 correlated with the transient expression of c-fos and egr1 ... The expression of the early response genes c-fos, egr1 , and cytokine-inducible SH2 protein (CIS) in response to SCF or Epo stimulation demonstrated that the transient expression of p42/44 correlated with the transient expression of c-fos and egr1 ... In addition, CIS was activated by Epo but not SCF
Bone-Larson et al., Am J Pathol 2000 (Acute Disease...) : Immunoneutralization studies revealed that the SCF stimulated release of monocyte chemoattractant protein-1 ( MCP-1 ) into the septic peritoneal cavity contributed to the protective effect of SCF in this model
Smothers et al., Mol Cell Biol 2000 : Here we demonstrate that in vivo SCF ( Met30p ) complex activity can be regulated by the abundance of Met30p
Ema et al., J Exp Med 2000 : In the presence of stem cell factor (SCF) , thrombopoietin (TPO) induced a first cell division of CD34 ( - ) KSL cells more efficiently than did interleukin (IL)-3 or IL-6
Duarte et al., Blood 2000 : However, SCF induces phosphorylation of STAT3 on serine727 ( ser727 ), which is necessary for maximal STAT transcriptional activity, and the combination of SCF and G-CSF leads to complete STAT3 phosphorylation on ser727 ... The pathways by which SCF and G-CSF lead to serine phosphorylation of STAT3 are distinct and are partially dependent on phosphatidylinositol-3 kinase and ERKs, pathways that are also necessary for the synergistic effects of SCF and G-CSF on proliferation and c-fos induction
Nishio et al., Exp Hematol 2001 : The Fas ligand (Fas-L) expressed on mature erythroblasts may induce apoptosis of more immature erythroid cells that express Fas, whereas stem cell factor (SCF) may prevent Fas mediated cell death in hematopoietic progenitor cells ... SCF also inhibited the activation of caspase-3 and caspase-8, without downregulation of the surface expression of Fas, suggesting that SCF prevents apoptosis through uncoupling of Fas ligation from subsequent caspase activation
Matsui et al., Int J Hematol 2000 : We have reported that simultaneous activation of glycoprotein (gp) 130 and c-kit signals by interleukin (IL)-6, soluble IL-6 receptor ( sIL-6R ), and stem cell factor (SCF) promotes proliferation of human hematopoietic progenitor cells and their differentiation into erythroid, myelocytic, and megakaryocytic cells
Tanaka et al., Biochimie 2001 : In this NF-kappaB signalling pathway, it becomes clear that the SCF ( Fbw1 ) activity is enhanced by a ubiquitin-like protein NEDD8 ( equivalent to Rub1 ) that modifies Cul-1 in a manner analogous to ubiquitylation, and consequently, IkappaBalpha proteolysis is induced
Ito et al., Fertil Steril 2001 : HGF increased the expression of SCF gene in granulosa cells, and SCF reciprocally increased the expression of HGF gene in theca cells ... HGF increased the expression of SCF gene in granulosa cells, and SCF reciprocally increased the expression of HGF gene in theca cells
Oliveira et al., Inflamm Res 2001 : In the present study we investigated the effect of SCF and/or IgE on histamine, TNF-alpha and chemokines released from bone marrow derived mast cells ( BMMC ) as well as chemokine receptor expression
Yamamoto et al., J Dermatol Sci 2001 (Fibrosis) : Here, we examined the effect of stem cell factor (SCF) , a mast cell growth factor, on MCP-1 gene expression in a human mast cell line, HMC-1, and as well as the effect of MCP-1 on alpha1 ( I ) collagen gene expression in human skin fibroblasts ... Stimulation with SCF further upregulated MCP-1 mRNA expression in a time- and dose dependent manner, and stimulation with 100 ng/ml SCF for 24 h induced a 3-fold increase of MCP-1 mRNA expression in HMC-1 cells as compared with unstimulated cells
Plo et al., Oncogene 2001 : In Ba/F3-wt and in Ba/F3-Kit cells, daunorubicin stimulated within 8-15 min neutral sphingomyelinase and ceramide production but not in SCF stimulated Ba/F3-Kit or in Ba/F3-KitDelta27 whereas all Ba/F3 cells were equally sensitive to exogenous cell-permeant C6-ceramide
Angchaisuksiri et al., Blood 2002 : However, medium conditioned by IL-2 stimulated, but not resting, NK cells ( NKCM ) contained a trypsin-sensitive factor capable of increasing 2- to 5-fold the number of polyploid megakaryocytes generated in vitro from IL-3 and SCF stimulated CD34 ( + ) cells
Bovolenta et al., Blood 2002 (Leukemia, Erythroblastic, Acute) : Neutralization experiments indicated that GM-CSF and IFN-gamma, but not SCF , were responsible for HTLV-2 induced STAT activation, whereas anti-GM-CSF antibodies greatly inhibited TF-1 cell proliferation
Prignano et al., J Eur Acad Dermatol Venereol 2001 : Moreover, TNF-alpha induced expression of CD54 by cells in the medium, but not by those retained in the sheets, whereas human SCF induced , dose dependently, expression of CD54 by cells in the sheets, but not from those in the medium ... Moreover, TNF-alpha induced expression of CD54 by cells in the medium, but not by those retained in the sheets, whereas human SCF induced, dose dependently, expression of CD54 by cells in the sheets, but not from those in the medium
Gyotoku et al., Arch Dermatol Res 2001 : Some IL-6 family cytokines enhanced the production of stem cell factor (SCF) , a potent mast cell growth factor, from NIH/3T3 fibroblasts, but the amount of SCF produced by NIH/3T3 fibroblasts was not paralleled by the mast cell growth-enhancement induced by the IL-6 family cytokines
Sitry et al., J Biol Chem 2002 : Previous studies have shown that the cyclin dependent kinase (Cdk) inhibitor p27 ( Kip1 ) is targeted for degradation by an SCF ( Skp2 ) ubiquitin ligase complex and that this process requires Cks1 , a member of the highly conserved Suc1/Cks family of cell cycle regulatory proteins
Maddens et al., Blood 2002 : Moreover, SCF stimulation inhibited IR-induced neutral sphingomyelinase (N-SMase) stimulation and ceramide production
Fujiki et al., Exp Hematol 2002 : Moreover, the endogenous production of TPO by cultured CD34 ( + ) IL-6R ( - ) cells in the presence of Epo+SCF+IL-9 was ruled out by reverse transcriptase polymerase chain reaction for TPO mRNA
Mendoza et al., J Biol Chem 2003 : The ubiquitin-like protein NEDD8 is essential for activity of SCF-like ubiquitin ligase complexes
Pintard et al., Curr Biol 2003 : Conjugation of the ubiquitin-like protein Nedd8 to the cullin subunit ( neddylation ) positively regulates activity of SCF complexes, most likely by increasing their affinity for the E2 conjugated to ubiquitin
Boer et al., Exp Hematol 2003 : STAT5 activation was examined in erythroid cell lines by analyzing the effects of EPO and SCF on STAT5 tyrosine phosphorylation, serine phosphorylation, DNA binding, and STAT5 mediated gene transactivation ... SCF pretreatment followed by EPO stimulation resulted in a 9.0-fold+/-0.9-fold increase in STAT5 transactivation, while SCF alone did not increase STAT5 transactivation ... SCF enhances the EPO mediated STAT5 transactivation by triggering a PKA/CREB dependent pathway
Yuan et al., Zhonghua Nan Ke Xue 2003 : It was reported that SCF/c-kit , Bcl-2 and Bcl-xl inhibited the apoptosis while caspase-3, Fas , Bax and clusterine stimulated it ... It was reported that SCF/c-kit , Bcl-2 and Bcl-xl inhibited the apoptosis while caspase-3, Fas, Bax and clusterine stimulated it
Kamura et al., Proc Natl Acad Sci U S A 2003 : Finally, the purified recombinant SCFSkp2 complex mediated p57Kip2 ubiquitylation in vitro in a manner dependent on the presence of the cyclin E-CDK2 complex
Jeong et al., Endocrinology 2003 : Our results show that dexamethasone potently regulates SCF induced migration, p38 MAPK activation, and inflammatory cytokine production through the expression of MKP-1 protein in RPMCs ... Our results show that dexamethasone potently regulates SCF induced migration, p38 MAPK activation, and inflammatory cytokine production through the expression of MKP-1 protein in RPMCs
Cope et al., Cell 2003 : Here, we summarize what is known about CSN, and discuss hypotheses for how CSN promotes the activity of SCF ubiquitin ligases
Kumar et al., EMBO J 2003 : SCF ( HOS ) ubiquitin ligase mediates the ligand induced down-regulation of the interferon-alpha receptor
Yu et al., Science 2003 : Induction of APOBEC3G ubiquitination and degradation by an HIV-1 Vif-Cul5-SCF complex
Ren et al., J Clin Invest 2003 : In vitro studies using primary mouse hepatocytes demonstrate that SCF causes hepatocyte proliferation and is induced by IL-6 and that treatment with anti-SCF antibodies inhibits IL-6 induced hepatocyte proliferation
Bayle et al., J Biol Chem 2004 : Ectopic expression of SOCS6 in Ba/F3-KIT cell line decreased cell proliferation in response to SCF but not SCF induced chemotaxis ... SOCS6 reduced SCF induced activation of ERK1/2 and p38 but not activation of AKT or STATs in Ba/F3, murine embryonic fibroblast (MEF), or COS-7 cells ... SOCS6 reduced SCF induced activation of ERK1/2 and p38 but not activation of AKT or STATs in Ba/F3, murine embryonic fibroblast (MEF), or COS-7 cells ... SOCS6 reduced SCF induced activation of ERK1/2 and p38 but not activation of AKT or STATs in Ba/F3, murine embryonic fibroblast (MEF), or COS-7 cells
Yamano et al., J Biol Chem 2004 : Here we show that fission yeast S phase cyclin Cig2 is ubiquitylated and degraded via both the SCF and the APC/C. Cig2 instability during G ( 2 ) and M phase is dependent upon the SCF complex , whereas the APC/C is responsible for Cig2 destruction during anaphase and G ( 1 ), thereby ensuring a spike pattern of Cig2 levels, peaking only at S phase ... SCF ( Pop1/Pop2 ) therefore regulates Cig2 levels in a dual manner, transcriptionally and post-translationally
Wan et al., J Biol Chem 2004 : Smad4 protein stability is regulated by ubiquitin ligase SCF beta-TrCP1
Kurland et al., Cancer Cell 2004 : Crashing waves of destruction : the cell cycle and APC ( Cdh1 ) regulation of SCF ( Skp2 )
Kondo et al., J Biol Chem 2004 : Rapid degradation of Cdt1 upon UV-induced DNA damage is mediated by SCFSkp2 complex
Carrabino et al., Brain Res Gene Expr Patterns 2004 : The CSN controls the function of the SCF ubiquitin-ligase complex through an enzymatic activity that removes the small ubiquitin-like molecule NEDD8 from the cullin component of the SCF and that requires subunit 5 of the CSN ( JAB1/CSN5 )
Zeng et al., Blood 2005 : We propose a negative feedback loop controlling the SCF-KIT signaling pathway, in which SCF activates KIT
Scholl et al., J Cancer Res Clin Oncol 2004 (Leukemia, Myeloid) : Stimulation with SCF leads to a significant increase in interaction between Kit and p85 as well as in receptor associated PI 3-kinase activity ... Stimulation with SCF leads to a significant increase in interaction between Kit and p85 as well as in receptor associated PI 3-kinase activity
Dai et al., Exp Hematol 2004 : The activations of caspase 3 and caspase 8 were almost completely blocked by SCF while CDK6 and the FLICE-inhibitory protein (FLIP) were increased
Sangrar et al., Exp Hematol 2004 (Anemia) : Fps/Fes was also activated in response to erythropoietin (EPO) and stem cell factor (SCF) , two critical factors in erythroid development ... Fps/Fes was also activated in response to erythropoietin (EPO) and stem cell factor (SCF) , two critical factors in erythroid development
Jin et al., Mol Reprod Dev 2005 : While SCF up-regulated the expression of the anti-apoptotic proteins Bcl-2 and Bcl-xL , it did the opposite to the pro-apoptotic factor Bax ... While SCF up-regulated the expression of the anti-apoptotic proteins Bcl-2 and Bcl-xL, it did the opposite to the pro-apoptotic factor Bax
Hue et al., Immunol Lett 2005 (Melanoma, Experimental) : IL-18 enhances SCF production of melanoma cells by regulating ROI and p38 MAPK activity ... Furthermore, inhibitors of p38 mitogen activated protein kinase ( MAPK ), such as SB203580, blocked enhanced SCF expression, indicating that p38 MAPK activity is required for IL-18 enhanced SCF production
Kohl et al., Blood 2005 (Acute Disease...) : All KIT exon 8 mutants induced receptor hyperactivation in response to stem cell factor (SCF) stimulation in terms of proliferation and resistance toward apoptotic cell death
Barbey et al., EMBO J 2005 : Cd2+ inhibits SCF ( Met30 ) activity through rapid dissociation of the F-box protein Met30 from the holocomplex
Drayer et al., Stem Cells 2005 : TPO stimulation of MO7e cells resulted in STAT5 transactivation, which could be enhanced 1.6-fold by costimulation with SCF, whereas SCF alone did not induce STAT5 transcriptional activity ... SCF induced enhancement of JAK2 phosphorylation was not affected by inhibition of Src kinase, suggesting that both JAK2 and Src kinase mediate STAT5 tyrosine phosphorylation
Tsang et al., J Leukoc Biol 2005 (Inflammation...) : Synergistic effect of SCF and TNF-alpha on the up-regulation of cell-surface expression of ICAM-1 on human leukemic mast cell line ( HMC ) -1 cells ... A synergistic effect of SCF and TNF-alpha on ICAM-1 expression was demonstrated ... Results indicated that SCF activated ERK, and TNF-alpha activated the p38 MAPK and NF-kappaB pathway ... Results indicated that SCF activated ERK , and TNF-alpha activated the p38 MAPK and NF-kappaB pathway ... Therefore, SCF and TNF-alpha acted through ERK and the NF-kappaB pathway to regulate the ICAM-1 expression and elicited the synergistic effect ... Therefore, SCF and TNF-alpha acted through ERK and the NF-kappaB pathway to regulate the ICAM-1 expression and elicited the synergistic effect
Dreier et al., Neuron 2005 : Instead, SCF ( LIN-23 ) regulates GLR-1 through the beta-catenin homolog BAR-1 and the TCF/Lef transcription factor homolog POP-1
Möller et al., Blood 2005 : SCF induced a rapid and transient phosphorylation of Akt ( protein kinase B ) and FOXO3a ... SCF induced a rapid and transient phosphorylation of Akt ( protein kinase B ) and FOXO3a
Wan et al., Am J Pathol 2005 (Adenocarcinoma...) : SCF(beta-TrCP1) controls Smad4 protein stability in pancreatic cancer cells
Zhang et al., Zhongguo Shi Yan Xue Ye Xue Za Zhi 2005 : This study was aimed to evaluate the effect of FL, SCF and TPO on CD34 ( + ) expansion
Benmaamar et al., Cell cycle (Georgetown, Tex.) 2005 : Involvement of the SCF complex in the control of Cdh1 degradation in S-phase
Hassel et al., J Cell Physiol 2006 : Abrogation of the c-kit/SCF dependent phosphorylation of BMPR-II at the Ser757 interfered with the cooperative effect of BMP2 and SCF
Blázquez-Domingo et al., Mol Cell Biol 2005 (Cell Transformation, Neoplastic) : SCF induced phosphoinositide-3-kinase (PI3K) appeared to control polysome recruitment of specific mRNAs associated with neoplastic transformation
Khanzada et al., Oncogene 2006 (Carcinoma, Small Cell...) : These responses correlated with the inhibition of stem cell factor (SCF) stimulated activation of extracellular signal regulated kinase ( Erk ), protein kinase B (PKB) and ribosomal S6 kinase by simvastatin ... These responses correlated with the inhibition of stem cell factor (SCF) stimulated activation of extracellular signal regulated kinase ( Erk ), protein kinase B (PKB) and ribosomal S6 kinase by simvastatin
Dang et al., J Neurosci Res 2005 (Neurofibromatoses) : Although the c-Kit ligand stem cell factor (SCF) induces the phosphorylation of protein kinase B ( or Akt ) and prevents apoptosis in Schwann cells, SCF has no effect on the proliferation or differentiation of Schwann cells
Kuhlmann et al., J Exp Med 2006 (Arrhythmias, Cardiac...) : G-CSF/SCF increased cardiomyocyte diameter, arteriogenesis, and expression of connexin43 in the border zone of the infarction
Bouchelouche et al., J Urol 2006 : Furthermore, IL-1beta and tumor necrosis factor-alpha alone induced significant release of MCP-1, IL-6 and SCF but in combination with IL-4 or IL-13 it induced greater secretion of MCP-1 , IL-6 and SCF ... Furthermore, IL-1beta and tumor necrosis factor-alpha alone induced significant release of MCP-1, IL-6 and SCF but in combination with IL-4 or IL-13 it induced greater secretion of MCP-1, IL-6 and SCF ... Furthermore, IL-1beta and tumor necrosis factor-alpha alone induced significant release of MCP-1, IL-6 and SCF but in combination with IL-4 or IL-13 it induced greater secretion of MCP-1, IL-6 and SCF ... Furthermore, IL-1beta and tumor necrosis factor-alpha alone induced significant release of MCP-1, IL-6 and SCF but in combination with IL-4 or IL-13 it induced greater secretion of MCP-1, IL-6 and SCF ... Furthermore, IL-1beta and tumor necrosis factor-alpha alone induced significant release of MCP-1, IL-6 and SCF but in combination with IL-4 or IL-13 it induced greater secretion of MCP-1, IL-6 and SCF ... Furthermore, IL-1beta and tumor necrosis factor-alpha alone induced significant release of MCP-1, IL-6 and SCF but in combination with IL-4 or IL-13 it induced greater secretion of MCP-1, IL-6 and SCF
Lin et al., Cell cycle (Georgetown, Tex.) 2006 : Importantly, our studies demonstrate that SCF ( xSkp2 ) supports the ubiquitination of Xic1 in a reconstituted in vitro ubiquitination assay and that this Xic1 ubiquitination does not require either CDK2-cyclins or Cks1 ... Importantly, our studies demonstrate that SCF ( xSkp2 ) supports the ubiquitination of Xic1 in a reconstituted in vitro ubiquitination assay and that this Xic1 ubiquitination does not require either CDK2-cyclins or Cks1
Wong et al., Allergy 2006 : Either SCF or TNF-alpha could induce release from HMC-1 cells of interleukin (IL)-8, monocyte chemoattractant protein (MCP)-1, regulated upon activation normal T-cell expressed and secreted ( RANTES ), and I-309, while SCF and TNF-alpha induced release of macrophage inflammatory protein (MIP)-1beta and interferon-gamma-inducible protein-10 (IP-10) , respectively ... Either SCF or TNF-alpha could induce release from HMC-1 cells of interleukin (IL)-8, monocyte chemoattractant protein (MCP)-1, regulated upon activation normal T-cell expressed and secreted ( RANTES ), and I-309, while SCF and TNF-alpha induced release of macrophage inflammatory protein (MIP)-1beta and interferon-gamma-inducible protein-10 (IP-10), respectively ... Either SCF or TNF-alpha could induce release from HMC-1 cells of interleukin (IL)-8, monocyte chemoattractant protein (MCP)-1, regulated upon activation normal T-cell expressed and secreted ( RANTES ), and I-309, while SCF and TNF-alpha induced release of macrophage inflammatory protein (MIP)-1beta and interferon-gamma-inducible protein-10 (IP-10), respectively ... Either SCF or TNF-alpha could induce release from HMC-1 cells of interleukin (IL)-8, monocyte chemoattractant protein (MCP)-1, regulated upon activation normal T-cell expressed and secreted ( RANTES ), and I-309, while SCF and TNF-alpha induced release of macrophage inflammatory protein (MIP)-1beta and interferon-gamma-inducible protein-10 (IP-10), respectively ... Either SCF or TNF-alpha could induce release from HMC-1 cells of interleukin (IL)-8, monocyte chemoattractant protein (MCP)-1, regulated upon activation normal T-cell expressed and secreted ( RANTES ), and I-309, while SCF and TNF-alpha induced release of macrophage inflammatory protein (MIP)-1beta and interferon-gamma-inducible protein-10 (IP-10), respectively ... Either SCF or TNF-alpha could induce release from HMC-1 cells of interleukin (IL)-8, monocyte chemoattractant protein (MCP)-1, regulated upon activation normal T-cell expressed and secreted ( RANTES ), and I-309, while SCF and TNF-alpha induced release of macrophage inflammatory protein (MIP)-1beta and interferon-gamma-inducible protein-10 (IP-10), respectively ... The induction of RANTES by SCF or TNF-alpha was mediated by ERK and NF-kappaB, respectively, and SCF induced MIP-1beta release was mediated by ERK ... The induction of RANTES by SCF or TNF-alpha was mediated by ERK and NF-kappaB, respectively, and SCF induced MIP-1beta release was mediated by ERK ... The induction of RANTES by SCF or TNF-alpha was mediated by ERK and NF-kappaB, respectively, and SCF induced MIP-1beta release was mediated by ERK ... The induction of RANTES by SCF or TNF-alpha was mediated by ERK and NF-kappaB , respectively, and SCF induced MIP-1beta release was mediated by ERK
De Schepper et al., Arch Dermatol Res 2006 (Cafe-au-Lait Spots...) : In this study we compared epidermal melanocyte and dermal mast cell numbers between four groups : control normal and control CALM skin, and NF1 normal and NF1 CALM skin and elaborated a possible role for stem cell factor (SCF) in CALM formation
Yoshikubo et al., Exp Hematol 2006 : Human UCB CD34 ( + ) cells were stably expanded on MS-5 stroma cells in the presence of SCF , FL, and TPO
Angers et al., Nat Cell Biol 2006 : Considered with evidence that the distinct Cullin-1 based SCF(beta-TrCP)complex regulates beta-catenin stability, our data on the stability of Dishevelled demonstrates that two distinct ubiquitin ligase complexes regulate the Wnt-beta-catenin pathway
Mao et al., Zhongguo Shi Yan Xue Ye Xue Za Zhi 2006 : SCF + FL + TPO combination not only promotes progenitor cells expansion but also upregulates the expression of CD49d and CXCR4 on CD34+ cells from cord blood ... SCF + FL + TPO combination not only promotes progenitor cells expansion but also upregulates the expression of CD49d and CXCR4 on CD34+ cells from cord blood
Liu et al., Biochem Biophys Res Commun 2006 : We hypothesize that CENP-E degradation is essential for faithful mitotic exit and the proteolysis of CENP-E is mediated by SCF via a direct Skp1 link
Noubissi et al., Nature 2006 : Overexpression of CRD-BP stabilizes betaTrCP1 mRNA and elevates betaTrCP1 levels ( both in cells and in vivo ), resulting in the activation of the Skp1-Cullin1-F-box protein (SCF) ( betaTrCP ) E3 ubiquitin ligase and in accelerated turnover of its substrates including IkappaB and beta-catenin ... Overexpression of CRD-BP stabilizes betaTrCP1 mRNA and elevates betaTrCP1 levels ( both in cells and in vivo ), resulting in the activation of the Skp1-Cullin1-F-box protein (SCF) ( betaTrCP ) E3 ubiquitin ligase and in accelerated turnover of its substrates including IkappaB and beta-catenin
van Drogen et al., Mol Cell 2006 : SCF ( hCdc4alpha ) binds a complex containing cyclin E, Cdk2, and the prolyl cis/trans isomerase Pin1 and promotes the activity of Pin1 without directly ubiquitylating cyclin E
Litz et al., Mol Cancer Ther 2006 (Carcinoma, Small Cell...) : In addition to hypoxia, peptide growth factors are known to regulate VEGF expression but the effect of stem cell factor (SCF) , the ligand for c-Kit, on VEGF expression has not been characterized ... SCF increased nuclear levels of the HIF-1alpha transcription factor , which correlated well with increased HIF-1alpha binding to a consensus hypoxia-responsive element ... These data indicate that activation of c-Kit by SCF leads to a predominantly HIF-1alpha mediated enhancement of VEGF expression and that inhibition of c-Kit signaling with imatinib could result in inhibition of tumor angiogenesis
Halfter et al., Cancer Res 2006 (Glioblastoma) : Degradation of p27 in late G ( 1 ) and S phase is initiated by the ubiquitin ligase complex SCF-Skp2/Cks1
Peschiaroli et al., Mol Cell 2006 : SCFbetaTrCP mediated degradation of Claspin regulates recovery from the DNA replication checkpoint response ... Thus, the SCFbetaTrCP dependent degradation of Claspin is necessary for the efficient and timely termination of the DNA replication checkpoint
Yao et al., J Mol Biol 2006 : Activation of ubiquitin ligase SCF ( Skp2 ) by Cks1 : insights from hydrogen exchange mass spectrometry
Megy et al., FEBS Lett 2006 (Neoplasms) : Phosphorylation of beta-Catenin at sites Ser33 and Ser37 on the DSGXXS motif is required for the interaction of beta-Catenin with the ubiquitin ligase SCF(beta-TrCP)
Zielke et al., EMBO Rep 2006 : We propose that Rca1 acts as an F-box protein in an as yet uncharacterized SCF complex , which promotes S-phase entry
Katiyar et al., Mol Cell Biol 2007 : Mutation of the c-Jun binding site abolished c-Jun mediated induction of the SCF promoter
Dolgachev et al., J Leukoc Biol 2007 : The data in the present study outline the signal transduction events that are induced by SCF in eosinophils and further demonstrate that MEK mediated signaling pathways are crucial for SCF induced CCL6 chemokine activation and eosinophil survival ... Subsequent analysis of CCL6 gene activation and production induced by SCF in the presence or absence of rather specific inhibitors for certain pathways demonstrated that the MEK/MAPK pathway but not the PI-3K pathway was crucial for the SCF induced CCL6 gene activation
Arcaro et al., Cell Signal 2007 (Carcinoma, Small Cell) : Lipid raft integrity was essential for Src activation in response to stem cell factor (SCF) and raft disruption selectively inhibited activation of protein kinase B (PKB)/Akt in response to SCF stimulation
Kim et al., Toxicol Appl Pharmacol 2007 : We also showed that SC-236 suppresses the SCF induced p38 MAPK activation in RPMCs ... We also showed that SC-236 suppresses the SCF induced p38 MAPK activation in RPMCs
Yasuda et al., Dig Dis Sci 2007 (Colorectal Neoplasms...) : In KIT positive lines, KIT was activated by stem cell factor (SCF)
Kondo et al., Blood 2007 : Moreover, KIT D816V and stem cell factor (SCF) activated wild-type KIT were found to induce Hsp32 promoter activity, expression of Hsp32 mRNA, and expression of the Hsp32 protein in Ba/F3 cells
van Kerkhof et al., J Biol Chem 2007 : The ubiquitin ligase SCF(betaTrCP) regulates the degradation of the growth hormone receptor
Zeuner et al., Cancer Res 2007 (Thrombocytopenia) : In chemotherapy treated megakaryocytes, SCF activated Akt, neutralized the mitochondrial apoptotic machinery, and inhibited caspase activity ... In chemotherapy treated megakaryocytes, SCF activated Akt , neutralized the mitochondrial apoptotic machinery, and inhibited caspase activity
Navarro et al., Anticancer Res 2007 (Bone Neoplasms...) : High expression of c-kit/SCF ( 2+, 3+ ) was detected in 28/45 cases of EFT ( 62.2 % ), whereas FAS-FAS-L and IGF-IR were observed in 16/45 ( 37.7 % ) and 9/45 ( 20 % ), respectively ... High expression of c-kit/SCF ( 2+, 3+ ) was detected in 28/45 cases of EFT ( 62.2 % ), whereas FAS-FAS-L and IGF-IR were observed in 16/45 ( 37.7 % ) and 9/45 ( 20 % ), respectively
Djouder et al., Mol Cell 2007 : Growth factor stimulation induces disassembly of URI/PP1gamma complexes through S6K1 mediated phosphorylation of URI at serine 371
Setoyama et al., Proc Natl Acad Sci U S A 2007 : We propose that Cdc2 and Plx1 sequentially phosphorylate CPEB and target it for SCF(beta-TrCP) dependent degradation in Xenopus oocytes
Murphy et al., J Immunol 2007 : Activation of PKD1 was inhibited by the combined PKD1 and protein kinase C ( PKC ) inhibitor Gö 6976 but not by broad-spectrum PKC inhibitors, including bisindolylmaleimide ( Bim ) I. Pam ( 3 ) CSK ( 4 ) and SCF also induced phosphorylation of heat shock protein 27, a known substrate of PKD1, which was also inhibited by Gö 6976 but not Bim I in BMMC
Kasamatsu et al., J Invest Dermatol 2008 : It has been demonstrated in other systems that a soluble form of m-KIT released from the cell surface ( s-KIT ) regulates SCF signaling, although there have been no reports pertaining to the existence and the biological role of s-KIT in melanocytes
Revai et al., Pediatr Infect Dis J 2008 (Hearing Disorders...) : Upper respiratory tract infections (URI) likely lead to acute otitis media (AOM) by causing Eustachian tube dysfunction which creates negative middle ear pressure
Han et al., Carcinogenesis 2008 (Neovascularization, Pathologic) : Hypoxia and epidermal growth factor receptor signal coexisted in the tumor microenvironment and might promote angiogenesis through HIF-1 alpha mediated upregulation of SCF and other angiogenic factors
Whitehurst et al., Mol Cell Biol 2008 : Surprisingly, we found that RASSF1A is also required to restrict SCF(betaTrCP) activity to allow G/S phase transition
Seki et al., J Cell Biol 2008 : Proteolysis of Bora requires the Plk1 kinase activity and is mediated by SCF-beta-TrCP
Hu et al., Am J Physiol Gastrointest Liver Physiol 2008 (Drug-Induced Liver Injury) : SCF inhibited APAP induced hepatocyte apoptosis and increased Bcl-2 and Bcl-xL expression, suggesting that this decrease in hepatocyte apoptosis is mediated through Bcl-2 and Bcl-xL ... SCF inhibited APAP induced hepatocyte apoptosis and increased Bcl-2 and Bcl-xL expression, suggesting that this decrease in hepatocyte apoptosis is mediated through Bcl-2 and Bcl-xL
Zhang et al., Proc Natl Acad Sci U S A 2008 : The fact that both decreased and increased CAND1-CUL1 interactions result in reduced SCF ( TIR1 ) activity in vivo strongly supports the hypothesis that CAND1 mediated cycling is required for optimal SCF function ... The fact that both decreased and increased CAND1-CUL1 interactions result in reduced SCF ( TIR1 ) activity in vivo strongly supports the hypothesis that CAND1 mediated cycling is required for optimal SCF function
Li et al., J Immunotoxicol 2008 : To examine if Egr-1 is required for SCF induced IL-13 expression, Egr-1-deficient BMMC were used
Moon et al., Free Radic Biol Med 2008 : Exogenous NO augmented COX-2 protein expression and increased COX-2 dependent PGD ( 2 ) generation in response to SCF , IL-10, and IL-1beta, or antigen activation in combination with IL-10 and IL-1beta after sensitization with IgE
Ang et al., J Biol Chem 2008 : In the presence of Plk2 , SPAR physically associated with the SCF(beta-TRCP) complex through a canonical phosphodegron
Pedersen et al., Biochem Biophys Res Commun 2008 (Neoplasms...) : In addition, SCF induced HIF-1alpha was transcriptionally active, and transcribed HIF-1 target genes such as VEGF, BNIP3, GLUT1 and DEC1, an effect that could be reversed by siRNA against HIF-1alpha
Jeon et al., Pigment Cell Melanoma Res 2009 : Here, we examined whether SCF induces ezrin/radixin/moesin (ERM) proteins phosphorylation as a downstream target of PI3K in melanocytes
Cen et al., J Biol Chem 2009 : Taken together, our findings indicate that HINT1 up-regulates cellular levels of p27 ( KIP1 ) by two mechanisms : 1 ) it inhibits its ubiquitylation by targeting the SCF ( SKP2 ) ubiquitin ligase complex, and 2 ) it inhibits the phosphorylation of p27 ( KIP1 ) by Src via inhibiting Src expression
Dolgachev et al., Am J Pathol 2009 (Lung Diseases) : Interestingly, SCF stimulated interleukin-31 receptor expression in bone marrow cells, whereas interleukin-31 strongly induced telomerase reverse transcriptase expression in fibroblasts
Reber et al., PloS one 2009 (Inflammation) : Ser276 phosphorylation of NF-kB p65 by MSK1 controls SCF expression in inflammation ... Finally, we demonstrate that both transfections of MSK1-KD and MSK1 siRNA -- but not the WT MSK1 or control siRNA -- downregulate the expression of SCF induced by IL-1ss
Lin et al., Nat Cell Biol 2009 (Neoplasm Metastasis...) : We find that Skp2 phosphorylation by Akt triggers SCF complex formation and E3 ligase activity
Liu et al., Biochem Biophys Res Commun 2009 (Ovarian Neoplasms) : MEK1 independent activation of MAPK and MEK1 dependent activation of p70 S6 kinase by stem cell factor (SCF) in ovarian cancer cells
Shirani et al., Cardiology journal 2009 (Aortic Diseases...) : Studies have suggested that flow mediated dilation (FMD) is impaired in SCF
Aerbajinai et al., Blood 2009 (MAP Kinase Signaling System) : We found that COUP-TFII expression is suppressed by SCF through phosphorylation of serine/threonine phosphatase ( PP2A ) and correlated well with fetal hemoglobin induction ... Our data show that SCF stimulates Erk1/2 MAPK signaling pathway, which regulates the downstream repressor COUP-TFII by inhibiting serine/threonine phosphatase 2A activity, and that decreased COUP-TFII expression resulted in gamma-globin reactivation in adult erythropoiesis
Bordeaux-Rego et al., Stem Cells Dev 2010 : The presence of SCF , Flt3-L, and TPO are essential for CD133+ and/or CD34+ cells ex vivo expansion ; however, IL-3 and IL-6 influence has not yet been clearly established
Li et al., Braz J Med Biol Res 2009 (Diabetes Mellitus, Experimental) : SCF expression was lower in the diabetic cortex than in the normal cortex ; however, insulin increased the expression of SCF in the diabetic cortex
Suyama et al., Oncol Rep 2010 (Lung Neoplasms...) : Using c-kit positive SCLC cells ( H209 and H69 cells ) and SCF as a model of the autocrine mechanisms, the effects of SCF , LY294002, PD98059 or STI571 on Akt and Erk were assessed by Western blot analysis ... Using c-kit positive SCLC cells ( H209 and H69 cells ) and SCF as a model of the autocrine mechanisms, the effects of SCF , LY294002, PD98059 or STI571 on Akt and Erk were assessed by Western blot analysis
Sánchez et al., J Med Food 2010 (Disease Models, Animal...) : In addition, SCF reduced plasma glucose and insulin , and as a consequence the insulin resistance was also decreased
Zeuner et al., Cell Death Differ 2011 : In cultures of primary human differentiating erythroblasts, we found that SCF induces an increase in the expression of Notch2 , a member of the Notch family implicated in the control of cell growth and differentiation ... SCF also induced the expression of Hes-1 and GATA-2 , which may contribute to transduce Notch2 signals in response to SCF ... SCF also induced the expression of Hes-1 and GATA-2, which may contribute to transduce Notch2 signals in response to SCF
Feuser et al., Mol Immunol 2011 : The combined challenge of mast cells with IL-4, SCF and Fc?RI cross linking substantially up-regulated activation of Akt , whereas blocking of Akt inhibited the pronounced production and release of IL-8 in response to the three mast cell agonists
Enomoto et al., Am J Pathol 2011 : In addition, we showed that MIF stimulated stem cell factor (SCF) release in keratinocytes ; however, MIF had no effect on the release of endothelin-1 or prostaglandin E2 in keratinocytes
Zhang et al., Cancer Lett 2011 (Anoxia...) : Furthermore, the expression of HIF-1a induced by SCF is not dependent on the oxygen level, but rather on both the PI3K/Akt and Ras/MEK/ERK signaling pathways ... In conclusion, under normoxic conditions, SCF/c-kit binding increases expression of HIF-1a through the PI3K/Akt and Ras/MEK/ERK pathways, and the accumulation of HIF-1a up-regulates expression of invasion related genes that augment the invasiveness of pancreatic cancer, a fatal cancer
Chen et al., Mol Cell Biol 2011 (Pseudomonas Infections) : Here we show that calmodulin inhibits a new SCF ( Skp1-Cullin-F-box ) E3 ligase component, FBXL2
Jeong et al., PloS one 2011 (Hypersensitivity...) : KMP6 and hesperidin inhibited SCF induced p38 MAPK activation
Dong et al., Pharmazie 2011 : Regulation of AMH and SCF expression in human granulosa cells by GnRH agonist and antagonist
Mita et al., Mol Cell Biol 2011 (Prostatic Neoplasms) : Repression of AR-mediated transcription is achieved, in part, by URI binding and regulation of androgen receptor trapped clone 27 ( Art-27 ), a previously characterized AR corepressor
Vaites et al., Mol Cell Biol 2011 (Cell Transformation, Neoplastic...) : Collectively, these data reveal a requisite role for the SCF ( Fbx4 ) E3 ubiquitin ligase in regulating cyclin D1 accumulation, consistent with tumor suppressive function in vivo
Rådinger et al., J Immunol 2011 : The decrease in stem cell factor (SCF) mediated survival in the GSK3ß knockdown HuMCs was reflected by enhancement of SCF withdrawal induced apoptosis, as determined by Annexin V staining and caspase cleavage, and this was associated with a pronounced reduction in SCF mediated phosphorylation of Src homology 2 domain containing phosphatase 2 and ERK1/2 and reduced expression of the antiapoptotic proteins Bcl-xl and Bcl-2 ... The decrease in stem cell factor (SCF) mediated survival in the GSK3ß knockdown HuMCs was reflected by enhancement of SCF withdrawal induced apoptosis, as determined by Annexin V staining and caspase cleavage, and this was associated with a pronounced reduction in SCF mediated phosphorylation of Src homology 2 domain containing phosphatase 2 and ERK1/2 and reduced expression of the antiapoptotic proteins Bcl-xl and Bcl-2 ... The decrease in stem cell factor (SCF) mediated survival in the GSK3ß knockdown HuMCs was reflected by enhancement of SCF withdrawal induced apoptosis, as determined by Annexin V staining and caspase cleavage, and this was associated with a pronounced reduction in SCF mediated phosphorylation of Src homology 2 domain containing phosphatase 2 and ERK1/2 and reduced expression of the antiapoptotic proteins Bcl-xl and Bcl-2
Nakajima et al., J Nat Med 2012 : Signaling analysis with immunoblots revealed that in human melanocytes or human melanoma cells treated with WSE, there was a marked deficiency in SCF stimulated phosphorylation of ERK, MITF and CREB , but not of Raf-1 and MEK ... Signaling analysis with immunoblots revealed that in human melanocytes or human melanoma cells treated with WSE, there was a marked deficiency in SCF stimulated phosphorylation of ERK , MITF and CREB, but not of Raf-1 and MEK ... Signaling analysis with immunoblots revealed that in human melanocytes or human melanoma cells treated with WSE, there was a marked deficiency in SCF stimulated phosphorylation of ERK, MITF and CREB, but not of Raf-1 and MEK ... Signaling analysis with immunoblots revealed that in human melanocytes or human melanoma cells treated with WSE, there was a marked deficiency in SCF stimulated phosphorylation of ERK, MITF and CREB, but not of Raf-1 and MEK
Piao et al., Differentiation 2012 : We also observe that SCF and G-CSF promote neuronal differentiation and inhibit astroglial differentiation at the early stage of differentiation
Hoellein et al., Mol Cell Biol 2012 : Cks1 is an activator of the SCF ( Skp2 ) ubiquitin ligase complex that targets the cell cycle inhibitor p27 ( Kip1 ) for degradation
Oliveira et al., J Endod 2012 (MAP Kinase Signaling System) : LPS induced SCF mRNA expression and production in OD-like cells occur via leukotriene production or cytokine and/or chemokine formation, activating the p42/44, p38, and PI3K pathways
Broxmeyer et al., Blood 2012 : In support of this, CD1d was required for the SCF and Flt3 ligand synergistic enhancement of CSF induction of HPC colony formation and for HPC response to myelosuppressive chemokines ... In support of this, CD1d was required for the SCF and Flt3 ligand synergistic enhancement of CSF induction of HPC colony formation and for HPC response to myelosuppressive chemokines
Nakajima et al., Arch Dermatol Res 2012 (Hyperpigmentation) : Real-time RT-PCR and western blotting of Ax-treated HEEs revealed that the SCF stimulated expression of tyrosinase (TYR), TYR related protein-1 (TYRP1), and Pmel17 , as well as microphthalmia associated transcription factor ( MITF ), is significantly suppressed by Ax at the transcriptional and translational levels ... Real-time RT-PCR and western blotting of Ax-treated HEEs revealed that the SCF stimulated expression of tyrosinase (TYR), TYR related protein-1 (TYRP1), and Pmel17, as well as microphthalmia associated transcription factor ( MITF ), is significantly suppressed by Ax at the transcriptional and translational levels ... Real-time RT-PCR and western blotting of Ax-treated HEEs revealed that the SCF stimulated expression of tyrosinase (TYR) , TYR related protein-1 (TYRP1), and Pmel17, as well as microphthalmia associated transcription factor ( MITF ), is significantly suppressed by Ax at the transcriptional and translational levels ... Real-time RT-PCR and western blotting of Ax-treated HEEs revealed that the SCF stimulated expression of tyrosinase (TYR), TYR related protein-1 (TYRP1) , and Pmel17, as well as microphthalmia associated transcription factor ( MITF ), is significantly suppressed by Ax at the transcriptional and translational levels ... Real-time RT-PCR and western blotting of Ax-treated HEEs revealed that the SCF stimulated expression of tyrosinase (TYR), TYR related protein-1 (TYRP1), and Pmel17, as well as microphthalmia associated transcription factor ( MITF ), is significantly suppressed by Ax at the transcriptional and translational levels
Jelly et al., Cancer cell international 2012 : Bcl-2 expression was significantly increased in the presence of adriamycin and SCF ( p? < ?0.038 ) and decreased in the presence of adriamycin and anti-SCF ... Bcl-2 expression was significantly increased in the presence of adriamycin and SCF ( p? < ?0.038 ) and decreased in the presence of adriamycin and anti-SCF ... However, in the presence of both adriamycin and SCF the expression of annexin V was decreased, but was significantly increased in the presence of adriamycin and anti-SCF ( p? < ?0.001 ), adriamycin, anti-SCF and SCF combined and adriamycin alone ... However, in the presence of both adriamycin and SCF the expression of annexin V was decreased, but was significantly increased in the presence of adriamycin and anti-SCF ( p? < ?0.001 ), adriamycin, anti-SCF and SCF combined and adriamycin alone ... Our results demonstrate that anti-SCF with low dose of adriamycin reduces Bcl-2 expression in MCF-7/AdrRes cells and increases annexin V expression in both MCF7/AdrRes and MCF-7 cells ... Our results demonstrate that anti-SCF with low dose of adriamycin reduces Bcl-2 expression in MCF-7/AdrRes cells and increases annexin V expression in both MCF7/AdrRes and MCF-7 cells
Bai et al., World J Gastroenterol 2012 (Gastrointestinal Neoplasms...) : To clarify the biological role of stem cell factor (SCF) mediated wild-type KIT receptor activation in gastrointestinal stromal tumor ( GIST ) growth
Wang et al., Neoplasia (New York, N.Y.) 2012 (Cell Transformation, Neoplastic...) : Furthermore, three independent laboratories including our own have demonstrated that the stability of DEPTOR is controlled by the SCF ( ß-TrCP ) E3 ubiquitin ligase and deregulated DEPTOR destruction might contribute to hyperactivation of mTOR in pathologic conditions including cancer
Enchev et al., Cell reports 2012 : Taken together, the results provide a model for how reciprocal regulation sensitizes CSN to the SCF assembly state and inhibits a catalytically competent SCF until a ubiquitination substrate drives its own degradation by displacing CSN, thereby promoting cullin neddylation and substrate ubiquitination
Bhaskaran et al., Mol Cell Biol 2013 : In vitro reconstitution showed that the role of SCF ( Fbw7a ) in cyclin E degradation, rather than ubiquitylation, is to serve as a cofactor of the prolyl cis-trans isomerase Pin1 in the isomerization of a noncanonical proline-proline bond in the cyclin E phosphodegron
Tanaka et al., Curr Pharm Des 2013 : The ubiquitin ligase activity of the SCF complex requires NEDD8 to covalently bind to cullins
Wakabayashi et al., J Dermatol Sci 2013 (Lentigo) : Real-time RT-PCR and Western blotting of these NCM treated HEEs unexpectedly revealed that the EDN1+SCF stimulated steady-state levels of tyrosinase (TYR), TYR related protein-1, dopachrome tautomerase and PMEL17 as well as microphthalmia associated transcription factor ( MITF ) were significantly attenuated at the transcriptional and translational levels without any cytotoxic effects on keratinocytes and melanocytes in the HEEs ... Real-time RT-PCR and Western blotting of these NCM treated HEEs unexpectedly revealed that the EDN1+SCF stimulated steady-state levels of tyrosinase (TYR), TYR related protein-1, dopachrome tautomerase and PMEL17 as well as microphthalmia associated transcription factor ( MITF ) were significantly attenuated at the transcriptional and translational levels without any cytotoxic effects on keratinocytes and melanocytes in the HEEs ... Real-time RT-PCR and Western blotting of these NCM treated HEEs unexpectedly revealed that the EDN1+SCF stimulated steady-state levels of tyrosinase (TYR) , TYR related protein-1, dopachrome tautomerase and PMEL17 as well as microphthalmia associated transcription factor ( MITF ) were significantly attenuated at the transcriptional and translational levels without any cytotoxic effects on keratinocytes and melanocytes in the HEEs ... Real-time RT-PCR and Western blotting of these NCM treated HEEs unexpectedly revealed that the EDN1+SCF stimulated steady-state levels of tyrosinase (TYR), TYR related protein-1, dopachrome tautomerase and PMEL17 as well as microphthalmia associated transcription factor ( MITF ) were significantly attenuated at the transcriptional and translational levels without any cytotoxic effects on keratinocytes and melanocytes in the HEEs ... Pre-treatment of cultured normal human melanocytes with the NCMs interrupted the EDN1+SCF induced stimulation of steady-state levels of MITF at the transcriptional and translational levels and TYR activity without any direct inhibitory effect on the catalytic activity of TYR in vitro ... Pre-treatment of cultured normal human melanocytes with the NCMs interrupted the EDN1+SCF induced stimulation of steady-state levels of MITF at the transcriptional and translational levels and TYR activity without any direct inhibitory effect on the catalytic activity of TYR in vitro
Lewis et al., Clin Exp Allergy 2013 : Mediator release induced by SCF was accompanied by the up-regulation of the activation marker, CD63
Shin et al., Exp Mol Med 2013 (Melanoma, Experimental) : Glyceollins inhibited melanin synthesis, as well as the expression and activity of tyrosinase induced by SCF , in a dose dependent manner in B16F10 melanoma cells
Lu et al., Food Chem Toxicol 2013 : HEE suppressed SCF/IL-10/LPS induced PGD2 generation, and concomitantly reduced COX-2 protein expression dose-dependently
Patel et al., Biometals 2013 : In addition, both ATP7A deficiency and neocuproine treatment had no effect on GM-CSF, IL-10, IL-11, IL-7, OSM, and SCF
Wiseman et al., J Biol Chem 2013 (Second Messenger Systems) : Proteasomal Degradation of Eukaryotic Elongation Factor-2 Kinase ( EF2K ) Is Regulated by cAMP-PKA Signaling and the SCFßTRCP Ubiquitin E3 Ligase ... Proteasomal Degradation of Eukaryotic Elongation Factor-2 Kinase ( EF2K ) Is Regulated by cAMP-PKA Signaling and the SCFßTRCP Ubiquitin E3 Ligase
Hurst et al., J Biol Chem 2013 : Here, we show that the Skp1/Cullin/F-box ubiquitin ligase SCF ( Cdc4 ) and the ubiquitin protease Ubp3 regulate Ste7 ubiquitination and signal specificity
Huang et al., PloS one 2013 : Unexpectedly however, csn3-3 plants are not defective in either the CSN mediated deneddylation of CUL1 or in SCF ( TIR1 ) -mediated degradation of Aux/IAA proteins
Cepeda et al., EMBO Mol Med 2013 (Breast Neoplasms) : SCF ( FBXO28 ) activity and stability are regulated during the cell cycle by CDK1/2 mediated phosphorylation of FBXO28 , which is required for its efficient ubiquitylation of MYC and downsteam enhancement of the MYC pathway
Fukushima et al., Cell reports 2013 : Here, we continue to report that SCF ( ß-TRCP ) reciprocally controls APC/Cdh1 activity by governing Cdh1 ubiquitination and subsequent degradation ... Here, we continue to report that SCF ( ß-TRCP ) reciprocally controls APC/Cdh1 activity by governing Cdh1 ubiquitination and subsequent degradation
Hirata et al., Development 1993 : We investigated the in vitro effect of stem cell factor (SCF) , the ligand for c-kit receptor, on DRGs. Recombinant murine SCF ( rmSCF ) induced the outgrowth of c-kit positive neurites from DRGs of normal ( +/+ ) embryos
Whetton et al., J Cell Biol 1994 (Second Messenger Systems) : Furthermore, M-CSF, but not SCF , can increase the levels of PKC alpha ( PKC alpha ) expression and stimulate the translocation of PKC alpha to the nucleus
Neta et al., J Immunol 1994 : SCF , unlike IL-1 does not induce hemopoietic CSFs and IL-6 or gene expression of a scavenging mitochondrial enzyme manganese superoxide dismutase in the bone marrow, suggesting that SCF and IL-1 radioprotect by distinct pathways
Blume-Jensen et al., J Biol Chem 1994 : In contrast, the SCF stimulated Kit/SCF-R associated efficiently with, and induced tyrosine phosphorylation of, the p85 alpha regulatory subunit of phosphatidyl inositide-3'-kinase ( PI-3'-kinase )
Tang et al., Mol Cell Biol 1994 : Conversely, the cytoplasmic kinase JAK2 is activated by IL-3 but not by SCF stimulation
Jhun et al., J Biol Chem 1995 : MATK-SH2, but not MATK-SH3, precipitated the tyrosine phosphorylated c-Kit ( molecular mass of 145 kDa ) in KL/SCF stimulated CMK cells
Shiohara et al., Blood 1993 : Primitive progenitors responding to SCF + IFN-gamma were suggested to be supported by SCF in the early stage of development and require IFN-gamma for subsequent growth
Koike et al., J Immunol 1993 (Second Messenger Systems) : Taken together, SCF induces the activation of PLD through the protein tyrosine kinase pathway without activation of phosphoinositide-specific phospholipase C
Sperr et al., Int Arch Allergy Immunol 1993 : A monoclonal antibody to SCF was found to inhibit the effects of SCF on MCs
Erickson-Miller et al., Stem Cells 1993 : We describe studies in which SCF stimulated murine CFU-Meg alone and in the presence of IL-3
Pallard et al., EMBO J 1995 (Leukemia, Megakaryoblastic, Acute) : In contrast, neither interferon (IFN)-gamma nor the mitogenic stem cell factor (SCF) activated STAT5 , although IFN-gamma did activate STAT1 in those cells
Reddy et al., Blood 1996 (Leukemia, Myeloid) : At low concentrations SCF caused a synergistic increase in IL-3 dependent induction of both CaM-BP68 and thymidine kinase activity ... At low concentrations SCF caused a synergistic increase in IL-3 dependent induction of both CaM-BP68 and thymidine kinase activity
O'Farrell et al., Blood 1996 : In B6M cells, both IL-3 and SCF stimulate phosphorylation of Shc and activation of the Ras, Raf-1, MAPK pathway ... Interestingly, IL-3 plus SCF synergistically activate MAPK
Weiler et al., Blood 1996 : These data demonstrate that SCF induces tyrosine phosphorylation of JAK2 and suggest that JAK2 is a component of the SCF signal transduction pathway
Langtimm-Sedlak et al., Dev Biol 1996 : Our data suggest that SCF affects neural crest cell development at multiple levels and that survival of melanogenic cells is mediated by a combination of SCF and a neurotropin, rather than by SCF alone
Pyatt et al., Mol Pharmacol 1996 : Support for this conclusion came from experiments demonstrating that 1 ) mutant mice strains constitutively lacking a SCF-responsive population of HPC [ White spotted ( W/WV ) and Steel ( SI/SId ) ] are unresponsive to xanthine/XO- and SCF induced enhancement of GM-CSF and 2 ) 3,4-epoxybutene, which selectively abrogates SCF synergy with GM-CSF, inhibits xanthine/XO induced enhancement
Nakahata et al., Cancer Chemother Pharmacol 1996 : The sIL-6R-IL-6 complex, but not sIL-6R or IL-6 alone, in the presence of stem-cell factor (SCF) produced dramatic increases in the populations of various cell lineages, including erythroid cells and various hematopoietic progenitors, in suspension culture
Thomas et al., Clin Exp Allergy 1996 : Changes in the level of mastocytoma mRNA for TNF alpha in response to stimulation with SCF or fibroblast conditioned media for up to 12 weeks were studied using Northern analysis and changes in the level of TNF alpha protein expression on immunoblot and immunocytochemistry
Imamura et al., Exp Hematol 1996 : Furthermore, LIF+SCF+IL-1 beta induced increased IL-3 and GM-CSF mRNA expression in hematopoietic cells but induced decreased macrophage inflammatory protein 1 alpha ( MIP1 alpha ) mRNA expression as compared with SCF+IL-1 beta +IL-3 ... Furthermore, LIF+SCF+IL-1 beta induced increased IL-3 and GM-CSF mRNA expression in hematopoietic cells but induced decreased macrophage inflammatory protein 1 alpha ( MIP1 alpha ) mRNA expression as compared with SCF+IL-1 beta +IL-3
Huss et al., Exp Mol Pathol 1995 : While differentiating, these cells start to express CD34 and DR. Differentiation is induced by stem cell factor (SCF) , while interleukin-6 (IL-6) inhibits differentiation, but promotes proliferation ... SCF induces a transient expression of p27kip-1, while no p27kip-1 is detectable in the presence of IL-6 or an anti-SCF monoclonal antibody ... SCF induces a transient expression of p27kip-1, while no p27kip-1 is detectable in the presence of IL-6 or an anti-SCF monoclonal antibody
Ramsfjell et al., Blood 1996 : SCF potently enhanced Tpo stimulated production of high-ploidy Mks from Lin- Sca-1+ progenitors, whereas the increased growth response obtained when combining Tpo with FL did not translate into increased Mk production
Dolzhanskiy et al., Blood 1997 : SCF alone had no effect on MK proliferation in the absence of TPO, but increased both MK number and CFU-MK by 1.5- to 2.0-fold in the presence of TPO
Jacobs-Helber et al., J Biol Chem 1997 (Leukemia, Erythroblastic, Acute) : A recent report ( Wu, H., Klingmuller, U., Besmer, P., and Lodish, H. F. ( 1995 ) Nature 377, 242-246 ) documents the interaction of the erythropoietin (EPO) receptor ( EPOR ) with the stem cell factor (SCF) receptor ( c-KIT ) and suggests that SCF acts through the EPOR ... However, SCF dependent phosphorylation of the EPOR did not initiate an EPO-like intracellular signal ... SCF induced proliferation, SHC phosphorylation, and activation of ERK-1 but did not activate the JAK/STAT pathway ... SCF induced proliferation, SHC phosphorylation, and activation of ERK-1 but did not activate the JAK/STAT pathway ... SCF stimulated SHC phosphorylation and ERK-1 activation independent of the EPOR in cells where the EPOR was down-regulated ; the presence of the EPOR appeared to facilitate SCF activation of SHC and ERK-1 ... SCF stimulated SHC phosphorylation and ERK-1 activation independent of the EPOR in cells where the EPOR was down-regulated ; the presence of the EPOR appeared to facilitate SCF activation of SHC and ERK-1
Ryan et al., Exp Hematol 1997 : These data indicate that SCF stimulation of Kit leads to activation of STAT-5 DNA binding with kinetics distinct from Epo mediated stimulation
Cardier et al., Stem Cells 1997 : Moreover, GM-CSF in the presence of SCF and IL-3 potentiates the HPP-CFC formation ( i.e., an increase of 3.1-fold compared to the effect induced by SCF+IL-3 ) with strong inhibitory effects on the number and size of megakaryocyte colonies
Grgurevich et al., Growth Factors 1997 : Previous reports demonstrating expression of Hyl and Matk in hematopoietic lineages led us to investigate the regulation of Lsk expression in response to stem cell factor (SCF) and granulocyte-macrophage colony stimulating factor ( GM-CSF ) in M07e, a human leukemic cell line ... These data demonstrate differential regulation of Csk family members by cytokines and suggest a role for Lsk/ Hyl/Matk in responses mediated by SCF and PMA
Kozawa et al., Eur J Biochem 1997 (Second Messenger Systems) : In contrast, phospholipase D ( PLD ) is activated in response to SCF in a dose dependent manner
Lorgeot et al., Cytokine 1997 : Interleukin 1alpha (IL-1alpha), IL-1beta, IL-3, IL-6, IL-8, tumour necrosis factor ( TNF-alpha ) and SCF ( both at 10 ng/ml ) stimulate LIF production
Joneja et al., Blood 1997 : In addition and in contrast to signaling in Mo7e and BM6 cell lines, in FDC2-ER cells SCF and Epo each were shown to rapidly activate Pim 1 gene expression ... These studies of SCF and Epo signaling in FDC2-wt-ER cells serve to functionally map the ERB1 region as a c-kit-interactive domain, suggest that Pim1 might contribute to SCF and Epo mitogenic synergy and support the notion that SCF and Epo may act in opposing ways during red cell differentiation
Deberry et al., Biochem J 1997 : We recently found that the Janus kinase Jak2 associates with c-kit and that SCF induces rapid and transient phosphorylation of Jak2 ... Here we present evidence that SCF activates the transcription factor Stat1 ... These results demonstrate that Stat1 is activated in response to SCF , and suggest that Stat1 is a component of the SCF signal-transduction pathway
Valent et al., Int Arch Allergy Immunol 1998 (Edema...) : The c-kit ligand SCF upregulates uPAR expression, and the release of tPA from MC ... The c-kit ligand SCF upregulates uPAR expression, and the release of tPA from MC
Qu et al., Int Arch Allergy Immunol 1998 : Expression of FGF-2 may be a ubiquitous feature of MC in other species in addition to humans, and can be selectively regulated by SCF , TGF-beta and TNF-alpha
Imokawa et al., Biochem J 1998 : HGF and SCF , but not bFGF, were markedly increased in culture medium in the presence of IL-1alpha , and this stimulatory effect was confined to young human fibroblasts
Harigae et al., Genes Cells 1998 : In contrast, GATA-1 protein was expressed in PMCs and BMMCs after culture in the presence of IL3 and SCF ... In contrast, GATA-1 protein was expressed in PMCs and BMMCs after culture in the presence of IL3 and SCF
Sato et al., J Biol Chem 1998 (Leukemia, Megakaryoblastic, Acute) : SCF activated the human EPO receptor promoter and induced EPO receptor gene expression ... SCF activated the human EPO receptor promoter and induced EPO receptor gene expression ... Given these results, we speculate that HML/SE cells acquired responsiveness to EPO via the EPO receptor induced by SCF
Muraguchi et al., Leukemia & lymphoma 1998 : Among the cytokines, IL-3, IL-6, and IL-7, but not IL-2, IL-4, SCF , GM-CSF induces RAG activation
Tisdale et al., Blood 1998 : Stem cell factor (SCF)/granulocyte colony stimulating factor ( G-CSF ) -mobilized and CD34 enriched PB cells were divided into two equal aliquots and transduced with one of two retroviral vectors carrying the neomycin-resistance gene ( neo ) for 4 days in the presence of interleukin-3 (IL-3) , IL-6, and SCF in the first 5 animals, IL-3/IL-6/SCF/Flt-3 ligand (FLT) in 2 subsequent animals, or IL-3/IL-6/SCF/FLT plus an autologous stromal monolayer ( STR ) in the final 2 ... Stem cell factor (SCF)/granulocyte colony stimulating factor ( G-CSF ) -mobilized and CD34 enriched PB cells were divided into two equal aliquots and transduced with one of two retroviral vectors carrying the neomycin-resistance gene ( neo ) for 4 days in the presence of interleukin-3 (IL-3), IL-6 , and SCF in the first 5 animals, IL-3/IL-6/SCF/Flt-3 ligand (FLT) in 2 subsequent animals, or IL-3/IL-6/SCF/FLT plus an autologous stromal monolayer ( STR ) in the final 2 ... Stem cell factor (SCF)/granulocyte colony stimulating factor ( G-CSF ) -mobilized and CD34 enriched PB cells were divided into two equal aliquots and transduced with one of two retroviral vectors carrying the neomycin-resistance gene ( neo ) for 4 days in the presence of interleukin-3 (IL-3), IL-6, and SCF in the first 5 animals, IL-3/IL-6/SCF/Flt-3 ligand (FLT) in 2 subsequent animals, or IL-3/IL-6/SCF/FLT plus an autologous stromal monolayer ( STR ) in the final 2
Sui et al., Blood 1998 : Together, the data suggest that synergistic activation of MAPK by SCF and EPO is essential for expanded erythropoiesis
Ishizuka et al., J Immunol 1998 : Coligation of SCFR augmented Fc epsilonRI mediated activation of MAP kinases, especially JNK activation, and SCF augmented Fc epsilonRI mediated TNF-alpha production in MC/9 cells, although SCF alone did not induce TNF-alpha production
Krystal et al., Cancer Res 1998 (Carcinoma, Small Cell...) : Stimulation of Kit with recombinant SCF resulted in a rapid 3-6-fold increase in the specific activity of Lck , which was similar in magnitude to the activation of Lck resulting from the cross linking of the T-cell receptor complex of Jurkat cells
Umekage et al., J Reprod Immunol 1998 : Results suggest that SCF produced in the decidua increases the expression of the IL-2Ralpha which is usually present in smaller amounts than other two IL-2R chains on decidual CD16- CD56bright NK cells, and thereby promotes the proliferation of these cells in response to low concentrations of IL-2, resulting in an increase of the high affinity IL-2Rs
Spyridonidis et al., Blood 1999 (Ascites...) : Expansion of clonogenic tumor cells was seen in the presence of SCF + IL-1beta + IL-3 + IL-6 + EPO, but was suppressed by TGF-beta1
Zhang et al., Blood 1999 : In the absence of TNFalpha, TGF-beta1 markedly suppressed the expression of CIITA and CCR7 mRNAs in GM-CSF + SCF stimulated Lin-c-kit+ HPCs at either day 6 or day 12 and induced the differentiation solely into monocytes/macrophages as evident in morphology, active phagocytic, and endocytic activities