◀ Back to SKP1
CUL1 — SKP1
Pathways - manually collected, often from reviews:
-
OpenBEL Selventa BEL large corpus:
Complex of CUL1-FBXW7-RBX1-SKP1
→
TP53
(increases)
Evidence: p53 might induce Cyclin E degradation via upregulation of Fbw7.
-
OpenBEL Selventa BEL large corpus:
HEY1
→
Complex of CUL1-FBXW7-RBX1-SKP1
(decreases)
Evidence: Notch4 degradation is impaired in Fbw7-/- cells. In contrast, the expression of Notch1, Notch2 and Notch3 is comparable among different genotypes. The finding that Fbw7-/- embryos exhibit severe defects in vascular development is likely due to the upregulation of the transcriptional repressor Hey-1, a downstream effector of Notch signaling.
-
OpenBEL Selventa BEL large corpus:
NOTCH4
→
Complex of CUL1-FBXW7-RBX1-SKP1
(decreases)
Evidence: The involvement of Fbw7 in the control of the Notch pathway is confirmed by the finding that Notch4 accumulates in Fbw7-deficient embryos, which die in utero at embryonic day 10.5 and manifest abnormal vascular development
-
OpenBEL Selventa BEL large corpus:
CDKN1A
→
Complex of CUL1-RBX1-SKP1-SKP2
(decreases, CUL1/RBX1/SKP1/SKP2 Activity)
Evidence: expression of Skp2 in the prostate gland induces hyperplasia, dysplasia and low- grade carcinoma accompanied by increased proliferation, downregulation of p27 and high levels of mitotic markers such as Ki67 and Cyclin B1
-
OpenBEL Selventa BEL large corpus:
CDKN1A
→
Complex of CUL1-RBX1-SKP1-SKP2
(decreases, CUL1/RBX1/SKP1/SKP2 Activity)
Evidence: targeted Skp2 expression to the T-lymphoid lineage markedly cooperates with the activated oncogene N-Ras. Compared to transgenic animals expressing activated N-Ras alone, Skp2 coexpression causes an acceleration in tumor onset, increased penetrance of lymphomagenesis and decreased survival rate. ectopic expression of Skp2 in nonadherent fibroblasts leads to entry into S phase, p27 downregulation and cell division, indicating that anchorage-dependent growth,a characteristic of nontransformed cell...
-
OpenBEL Selventa BEL large corpus:
MYC
→
Complex of CUL1-RBX1-SKP1-SKP2
(increases, CUL1/RBX1/SKP1/SKP2 Activity)
Evidence: It has been reported that during the G1 to S transition of the cell cycle, Skp2 regulates the ubiquitinylation and stability of the transcription factor c-Myc, an oncoprotein that plays a major role in oncogenesis Skp2 enhances c-Myc-induced S phase transition and activates c-Myc transcriptional activity.
-
KEGG Cell cycle:
Complex of CUL1-RBX1-SKP1-SKP2
→
Complex of CCNE1-CCNE2-CDK2
(protein-protein, inhibition)
-
KEGG Cell cycle:
Complex of CUL1-RBX1-SKP1-SKP2
→
CDKN1B/CDKN1C
(protein-protein, inhibition)
-
KEGG Cell cycle:
Complex of CUL1-RBX1-SKP1-SKP2
→
CDKN1A
(protein-protein, inhibition)
-
KEGG Oocyte meiosis:
Complex of BTRC-CUL1-FBXW11-RBX1-SKP1
→
FBXO5
(protein-protein, inhibition)
-
KEGG Oocyte meiosis:
Complex of BTRC-CUL1-FBXW11-RBX1-SKP1
→
FBXO43
(protein-protein, inhibition)
-
KEGG Oocyte meiosis:
Complex of BTRC-CUL1-FBXW11-RBX1-SKP1
→
FBXO43
(protein-protein, inhibition)
-
KEGG TGF-beta signaling pathway:
Complex of CUL1-RBX1-SKP1
→
SMAD2/SMAD3
(protein-protein, inhibition)
-
KEGG Circadian rhythm - mammal:
Complex of BTRC-CUL1-FBXW11-RBX1-SKP1
→
PER1/PER2/PER3
(protein-protein, inhibition)
-
NCI Pathway Database Regulation of nuclear beta catenin signaling and target gene transcription:
TCF4/beta catenin complex (TCF7L2-CTNNB1)
→
SCF complex (BTRC-SKP1-CUL1)
(modification, collaborate)
Finkbeiner et al., Cell cycle (Georgetown, Tex.) 2008
Evidence: physical interaction
-
NCI Pathway Database Regulation of nuclear beta catenin signaling and target gene transcription:
TCF4/beta catenin complex (TCF7L2-CTNNB1)
→
TCF4/beta catenin/TRRAP/SCF complex (TCF7L2-CTNNB1-TRRAP-BTRC-CUL1-SKP1)
(modification, collaborate)
Finkbeiner et al., Cell cycle (Georgetown, Tex.) 2008
Evidence: physical interaction
-
NCI Pathway Database Regulation of nuclear beta catenin signaling and target gene transcription:
TRRAP (TRRAP)
→
SCF complex (BTRC-SKP1-CUL1)
(modification, collaborate)
Finkbeiner et al., Cell cycle (Georgetown, Tex.) 2008
Evidence: physical interaction
-
NCI Pathway Database Regulation of nuclear beta catenin signaling and target gene transcription:
TRRAP (TRRAP)
→
TCF4/beta catenin/TRRAP/SCF complex (TCF7L2-CTNNB1-TRRAP-BTRC-CUL1-SKP1)
(modification, collaborate)
Finkbeiner et al., Cell cycle (Georgetown, Tex.) 2008
Evidence: physical interaction
-
NCI Pathway Database Regulation of nuclear beta catenin signaling and target gene transcription:
SCF complex (BTRC-SKP1-CUL1)
→
TCF4/beta catenin/TRRAP/SCF complex (TCF7L2-CTNNB1-TRRAP-BTRC-CUL1-SKP1)
(modification, collaborate)
Finkbeiner et al., Cell cycle (Georgetown, Tex.) 2008
Evidence: physical interaction
-
NCI Pathway Database Notch signaling pathway:
SCF/SEL10 complex (BTRC-SKP1-CUL1-FBXW7)
→
NICD (NOTCH1)
(proteasomal ubiquitin-dependent protein catabolic process, activates)
Gupta-Rossi et al., J Biol Chem 2001, Wu et al., Mol Cell Biol 2001, Malyukova et al., Cancer Res 2007
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database Degradation of beta catenin:
SCF complex (BTRC-SKP1-CUL1)
→
APC/beta catenin complex (APC-CTNNB1)
(modification, activates)
Hart et al., Curr Biol 1999, Kitagawa et al., EMBO J 1999, Winer et al., J Biol Chem 2006, Seo et al., Cell Signal 2009, Su et al., Mol Cell 2008
Evidence: assay, physical interaction
-
NCI Pathway Database Degradation of beta catenin:
SCF complex (BTRC-SKP1-CUL1)
→
APC (APC)
(modification, activates)
Hart et al., Curr Biol 1999, Kitagawa et al., EMBO J 1999, Winer et al., J Biol Chem 2006, Seo et al., Cell Signal 2009, Su et al., Mol Cell 2008
Evidence: assay, physical interaction
-
Reactome Reaction:
CUL1
→
SKP1
(reaction)
Ganoth et al., Nat Cell Biol 2001, Oberg et al., J Biol Chem 2001, Wu et al., Mol Cell Biol 2001, Tedesco et al., Genes Dev 2002, Cohen et al., Mol Cell Biol 2004, Hansen et al., Mol Biol Cell 2004, Fryer et al., Mol Cell 2004, Welcker et al., Nat Rev Cancer 2008, Seki et al., J Cell Biol 2008
-
Reactome Reaction:
CUL1
→
SKP1
(direct_complex)
Oberg et al., J Biol Chem 2001, Wu et al., Mol Cell Biol 2001, Fryer et al., Mol Cell 2004, Welcker et al., Nat Rev Cancer 2008
Protein-Protein interactions - manually collected from original source literature:
Studies that report less than 10 interactions are marked with *
-
IRef Bind Interaction:
Complex of 11 proteins
Gstaiger et al., Science 2003
-
IRef Bind Interaction:
Complex of 25 proteins
Bouwmeester et al., Nat Cell Biol 2004
-
IRef Bind Interaction:
Complex of 11 proteins
Bouwmeester et al., Nat Cell Biol 2004
-
IRef Bind Interaction:
Complex of CUL1-SKP1-FBXO18-RBX1
Kim et al., J Biol Chem 2002*
-
IRef Bind Interaction:
SKP1
—
CUL1
Zheng et al., Nature 2002*
-
IRef Bind_translation Interaction:
SKP1
—
CUL1
(x-ray crystallography)
Zheng et al., Nature 2002*
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Bennett et al., Cell 2010
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Lyapina et al., Proc Natl Acad Sci U S A 1998*
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(direct interaction, two hybrid)
Lyapina et al., Proc Natl Acad Sci U S A 1998*
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Furukawa et al., Mol Cell Biol 2000*
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(direct interaction, pull down)
Lyapina et al., Proc Natl Acad Sci U S A 1998*
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Liu et al., Mol Cell 2002*
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(direct interaction, pull down)
Liu et al., Mol Cell 2002*
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(direct interaction, pull down)
Yu et al., Proc Natl Acad Sci U S A 1998*
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Yu et al., Proc Natl Acad Sci U S A 1998*
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Postow et al., Cell cycle (Georgetown, Tex.) 2013*
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Cordero-Espinoza et al., Biology open 2013*
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Latres et al., Oncogene 1999
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Min et al., J Biol Chem 2003
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Staropoli et al., Neuron 2003*
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Lisztwan et al., EMBO J 1998*
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(direct interaction, two hybrid)
Ohta et al., Mol Cell 1999
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(association, x-ray crystallography)
Zheng et al., Nature 2002*
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(direct interaction, pull down)
Wang et al., J Biol Chem 2004*
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Bloom et al., Cell division 2006
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Kumar et al., Cancer Res 2005*
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Megumi et al., Genes Cells 2005*
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(colocalization, biochemical)
Tan et al., Mol Cell 1999
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Arai et al., Proc Natl Acad Sci U S A 2003
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Wu et al., Mol Cell Biol 2000*
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Read et al., Mol Cell Biol 2000
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(direct interaction, pull down)
Mao et al., J Biol Chem 2011
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Lin et al., Nat Cell Biol 2009*
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Gao et al., Mol Cell 2010*
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Yano et al., Mol Biol Cell 2008*
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Banerjee et al., J Biol Chem 2010*
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Chua et al., PloS one 2011*
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(direct interaction, pull down)
Zhang et al., EMBO J 2008*
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Winston et al., Genes Dev 1999
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Oshikawa et al., Biochem Biophys Res Commun 2003*
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Lee et al., Mol Cell Proteomics 2011*
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Emberley et al., J Biol Chem 2012
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Michel et al., Cell Growth Differ 1998*
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(physical association, affinity chromatography technology)
Gastaldello et al., Journal of molecular cell biology 2012
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(direct interaction, two hybrid)
Michel et al., Cell Growth Differ 1998*
-
MIPS CORUM Ubiquitin E3 ligase (SKP1A, SKP2, CUL1, RBX1):
Ubiquitin E3 ligase (SKP1A, SKP2, CUL1, RBX1) complex (CUL1-RBX1-SKP1-SKP2)
Tan et al., Mol Cell 1999
-
MIPS CORUM Ubiquitin E3 ligase (FBXW11, SKP1A, CUL1, RBX1):
Ubiquitin E3 ligase (FBXW11, SKP1A, CUL1, RBX1) complex (CUL1-FBXW11-RBX1-SKP1)
Tan et al., Mol Cell 1999
-
MIPS CORUM Ubiquitin E3 ligase (FBXW7, CUL1, SKP1A, RBX1):
Ubiquitin E3 ligase (FBXW7, CUL1, SKP1A, RBX1) complex (CUL1-FBXW7-RBX1-SKP1)
Wu et al., Mol Cell Biol 2001
-
MIPS CORUM Ubiquitin E3 ligase (FBXW7, CUL1, SKP1A, RBX1):
Ubiquitin E3 ligase (FBXW7, CUL1, SKP1A, RBX1) complex (CUL1-FBXW7-RBX1-SKP1)
Strohmaier et al., Nature 2001*
-
MIPS CORUM Neddylin ligase (FBXO11, SKP1, CUL1, RBX1):
Neddylin ligase (FBXO11, SKP1, CUL1, RBX1) complex (CUL1-FBXO11-RBX1-SKP1)
Abida et al., J Biol Chem 2007
-
MIPS CORUM Ubiquitin E3 ligase (NFKBIA, FBXW11, BTRC, CUL1, SKP1A):
Ubiquitin E3 ligase (NFKBIA, FBXW11, BTRC, CUL1, SKP1A) complex (BTRC-CUL1-FBXW11-NFKBIA-SKP1)
Suzuki et al., J Biol Chem 2000
-
MIPS CORUM Ubiquitin E3 ligase (CDC34, NEDD8, BTRC, CUL1, SKP1A, RBX1):
Ubiquitin E3 ligase (CDC34, NEDD8, BTRC, CUL1, SKP1A, RBX1) complex (BTRC-CUL1-NEDD8-RBX1-SKP1)
Read et al., Mol Cell Biol 2000
-
MIPS CORUM Ubiquitin E3 ligase (SMAD3, BTRC, CUL1, SKP1A, RBX1):
Ubiquitin E3 ligase (SMAD3, BTRC, CUL1, SKP1A, RBX1) complex (BTRC-CUL1-RBX1-SKP1-SMAD3)
Fukuchi et al., Mol Biol Cell 2001
-
MIPS CORUM Ubiquitin E3 ligase (FBXO18, SKP1A, CUL1, RBX1):
Ubiquitin E3 ligase (FBXO18, SKP1A, CUL1, RBX1) complex (CUL1-FBXO18-RBX1-SKP1)
Kim et al., J Biol Chem 2002*
-
MIPS CORUM Ubiquitin E3 ligase (NIPA, SKP1A, CUL1, RBX1):
Ubiquitin E3 ligase (NIPA, SKP1A, CUL1, RBX1) complex (CUL1-RBX1-SKP1-ZC3HC1)
Bassermann et al., Cell 2005*
-
MIPS CORUM Ubiquitin E3 ligase (SKP1A, SKP2, CUL1):
Ubiquitin E3 ligase (SKP1A, SKP2, CUL1) complex (CUL1-SKP1-SKP2)
Min et al., J Biol Chem 2003
-
MIPS CORUM Ubiquitin E3 ligase (SKP1A, BTRC, CUL1):
Ubiquitin E3 ligase (SKP1A, BTRC, CUL1) complex (BTRC-CUL1-SKP1)
Winston et al., Genes Dev 1999
-
MIPS CORUM Ubiquitin E3 ligase (CSN1, CSN8, HRT1, SKP1, SKP2, CUL1, CUL2, CUL3):
Ubiquitin E3 ligase (CSN1, CSN8, HRT1, SKP1, SKP2, CUL1, CUL2, CUL3) complex (COPS8-CUL1-CUL2-CUL3-GPS1-RBX1-SKP1-SKP2)
Lyapina et al., Science 2001
-
MIPS CORUM Ubiquitin E3 ligase (NFKBIA, BTRC, CUL1, SKP1A):
Ubiquitin E3 ligase (NFKBIA, BTRC, CUL1, SKP1A) complex (BTRC-CUL1-NFKBIA-SKP1)
Suzuki et al., J Biol Chem 2000
-
MIPS CORUM Ubiquitin E3 ligase (NFKBIA, FBXW11, CUL1, SKP1A):
Ubiquitin E3 ligase (NFKBIA, FBXW11, CUL1, SKP1A) complex (CUL1-FBXW11-NFKBIA-SKP1)
Suzuki et al., J Biol Chem 2000
-
MIPS CORUM Ubiquitin E3 ligase (CRY1, SKP1A, CUL1, FBXL3):
Ubiquitin E3 ligase (CRY1, SKP1A, CUL1, FBXL3) complex (CRY1-CUL1-FBXL3-SKP1)
Busino et al., Science 2007
-
MIPS CORUM Ubiquitin E3 ligase (CRY2, SKP1A, CUL1, FBXL3):
Ubiquitin E3 ligase (CRY2, SKP1A, CUL1, FBXL3) complex (CRY2-CUL1-FBXL3-SKP1)
Busino et al., Science 2007
-
MIPS CORUM Ubiquitin E3 ligase (CUL1, RBX1, SKP1A):
Ubiquitin E3 ligase (CUL1, RBX1, SKP1A) complex (CUL1-RBX1-SKP1)
Watanabe et al., Proc Natl Acad Sci U S A 2004
-
MIPS CORUM p27-cyclinE-Cdk2 - Ubiquitin E3 ligase (SKP1A, SKP2, CUL1, CKS1B, RBX1) complex:
p27-cyclinE-Cdk2 - Ubiquitin E3 ligase (SKP1A, SKP2, CUL1, CKS1B, RBX1) complex complex (CCNE1-CDK2-CDKN1B-CKS1B-CUL1-RBX1-SKP1-SKP2)
Xu et al., J Biol Chem 2007
-
MIPS CORUM Ubiquitin E3 ligase (SKP1A, SKP2, CUL1, CKS1B, RBX1):
Ubiquitin E3 ligase (SKP1A, SKP2, CUL1, CKS1B, RBX1) complex (CKS1B-CUL1-RBX1-SKP1-SKP2)
Xu et al., J Biol Chem 2007
-
MIPS CORUM Ubiquitin E3 ligase (SKP1A, FBXW2, CUL1):
Ubiquitin E3 ligase (SKP1A, FBXW2, CUL1) complex (CUL1-FBXW2-SKP1)
Yang et al., J Biol Chem 2005*
-
MIPS CORUM Heterotrimeric SKP1-CUL1-ROC1 complex:
Heterotrimeric SKP1-CUL1-ROC1 complex complex (CUL1-RBX1-SKP1)
Kim et al., Nucleic Acids Res 2004*
-
MIPS CORUM TNF-alpha/NF-kappa B signaling complex (CHUK, BTRC, NFKB2, PPP6C, REL, CUL1, IKBKE, SAPS2, SAPS1, ANKRD28, RELA, SKP1):
TNF-alpha/NF-kappa B signaling complex (CHUK, BTRC, NFKB2, PPP6C, REL, CUL1, IKBKE, SAPS2, SAPS1, ANKRD28, RELA, SKP1) complex (ANKRD28-BTRC-CHUK-CUL1-IKBKE-NFKB2-PPP6C-REL-RELA-PPP6R1-PPP6R2-SKP1)
Bouwmeester et al., Nat Cell Biol 2004
-
MIPS CORUM TNF-alpha/NF-kappa B signaling complex 5:
TNF-alpha/NF-kappa B signaling complex 5 complex (CD3EAP-CHUK-CUL1-FBXW11-IKBKB-IKBKG-IQGAP2-KPNA2-LRPPRC-MCC-MTIF2-NFKB1-NFKB2-NFKBIB-PDCD2-POLR1A-POLR1B-POLR1D-POLR1E-POLR2H-POLR2L-RASAL2-REL-RELA-SKP1)
Bouwmeester et al., Nat Cell Biol 2004
-
MIPS CORUM Ubiquitin E3 ligase (SKP1A, SKP2, CUL1):
Ubiquitin E3 ligase (SKP1A, SKP2, CUL1) complex (CUL1-SKP1-SKP2)
Yu et al., Proc Natl Acad Sci U S A 1998*
-
MIPS CORUM Ubiquitin E3 ligase (FBXO31, SKP1A, CUL1, RBX1):
Ubiquitin E3 ligase (FBXO31, SKP1A, CUL1, RBX1) complex (CUL1-FBXO31-RBX1-SKP1)
Kumar et al., Cancer Res 2005*
-
MIPS CORUM URI complex (Unconventional prefoldin RPB5 Interactor):
URI complex (Unconventional prefoldin RPB5 Interactor) complex (CUL1-PFDN2-POLR2E-URI1-RUVBL1-RUVBL2-SKP1-SKP2-STAP1)
Gstaiger et al., Science 2003
-
MIPS CORUM Ubiquitin E3 ligase (Skp1A, Skp2, Cul1, Rbx1):
Ubiquitin E3 ligase (Skp1A, Skp2, Cul1, Rbx1) complex (CUL1-RBX1-SKP1-SKP2)
Zheng et al., Nature 2002*
-
IRef Corum Interaction:
Complex of 19 proteins
(association, molecular sieving)
Gstaiger et al., Science 2003
-
IRef Corum Interaction:
Complex of NFKBIA-SKP1-CUL1-FBXW11
(association, anti tag coimmunoprecipitation)
Suzuki et al., J Biol Chem 2000
-
IRef Corum Interaction:
Complex of RBX1-SKP1-SMAD3-CUL1-BTRC
(association, coimmunoprecipitation)
Fukuchi et al., Mol Biol Cell 2001
-
IRef Corum Interaction:
Complex of CUL1-SKP1-RBX1-NEDD8-BTRC
(association, anti tag coimmunoprecipitation)
Read et al., Mol Cell Biol 2000
-
IRef Corum Interaction:
Complex of RBX1-FBXO31-CUL1-SKP1
(association, anti tag coimmunoprecipitation)
Kumar et al., Cancer Res 2005*
-
IRef Corum Interaction:
Complex of NFKBIA-SKP1-CUL1-FBXW11-BTRC
(association, anti tag coimmunoprecipitation)
Suzuki et al., J Biol Chem 2000
-
IRef Corum Interaction:
Complex of SKP1-ZC3HC1-RBX1-CUL1
(association, anti tag coimmunoprecipitation)
Bassermann et al., Cell 2005*
-
IRef Corum Interaction:
Complex of FBXO18-SKP1-CUL1-RBX1
(association, anti tag coimmunoprecipitation)
Kim et al., J Biol Chem 2002*
-
IRef Corum Interaction:
Complex of 13 proteins
(association, tandem affinity purification)
Bouwmeester et al., Nat Cell Biol 2004
-
IRef Corum Interaction:
Complex of RBX1-SKP1-CKS1B-CDKN1B-CDK2-CCNE1-SKP2-CUL1
(association, anti tag coimmunoprecipitation)
Xu et al., J Biol Chem 2007
-
IRef Corum Interaction:
Complex of 26 proteins
(association, tandem affinity purification)
Bouwmeester et al., Nat Cell Biol 2004
-
IRef Corum Interaction:
Complex of SKP1-CUL1-CRY1-FBXL3
(association, anti tag coimmunoprecipitation)
Busino et al., Science 2007
-
IRef Corum Interaction:
Complex of CRY2-CUL1-SKP1-FBXL3
(association, anti tag coimmunoprecipitation)
Busino et al., Science 2007
-
IRef Corum Interaction:
Complex of RBX1-SKP1-CUL1-RBX1-SKP1-CUL1
(association, coimmunoprecipitation)
Kim et al., Nucleic Acids Res 2004*
-
IRef Corum Interaction:
Complex of RBX1-SKP1-GPS1-SKP2-CUL1-CUL2-CUL3-COPS8
(association, far western blotting)
Lyapina et al., Science 2001
-
IRef Corum Interaction:
Complex of RBX1-SKP1-CUL1-FBXW7-RBX1-SKP1-CUL1-FBXW7
(association, coimmunoprecipitation)
Wu et al., Mol Cell Biol 2001
-
IRef Corum Interaction:
Complex of FBXW2-CUL1-SKP1
(association, anti tag coimmunoprecipitation)
Yang et al., J Biol Chem 2005*
-
IRef Corum Interaction:
Complex of NFKBIA-SKP1-CUL1-BTRC
(association, anti tag coimmunoprecipitation)
Suzuki et al., J Biol Chem 2000
-
IRef Corum Interaction:
Complex of RBX1-SKP1-CUL1-FBXW11
(association, affinity chromatography technology)
Tan et al., Mol Cell 1999
-
IRef Dip Interaction:
Complex of SKP1-CUL1-RBX1
(anti tag coimmunoprecipitation)
Sonnberg et al., Proc Natl Acad Sci U S A 2008*
-
IRef Dip Interaction:
Complex of CUL1-SKP1
(anti tag coimmunoprecipitation)
Sonnberg et al., Proc Natl Acad Sci U S A 2008*
-
IRef Dip Interaction:
Complex of BCL6-BCL6-SKP1-SKP1-CUL1-CUL1-FBXO11-FBXO11
(anti tag coimmunoprecipitation)
Duan et al., Nature 2012
-
IRef Dip Interaction:
Complex of SKP1-CUL1-FBXO11
(anti tag coimmunoprecipitation)
Duan et al., Nature 2012
-
IRef Dip Interaction:
Complex of BCL6-SKP1-FBXO11-CUL1-RBX1
(anti bait coimmunoprecipitation)
Duan et al., Nature 2012
-
IRef Dip Interaction:
Complex of CCND1-CUL1-SKP1-FBXO31
(anti tag coimmunoprecipitation)
Santra et al., Nature 2009
-
IRef Dip Interaction:
Complex of CUL1-CCP110-CCP110-CCNF-SKP1-CCNF-CUL1-SKP1
(anti tag coimmunoprecipitation)
D'Angiolella et al., Nature 2010
-
IRef Dip Interaction:
Complex of 18 proteins
(anti tag coimmunoprecipitation)
Litterman et al., PLoS Biol 2011
-
IRef Dip Interaction:
Complex of 17 proteins
(anti tag coimmunoprecipitation)
Sonnberg et al., Proc Natl Acad Sci U S A 2008*
-
IRef Dip Interaction:
Complex of CUL1-SKP1
(anti tag coimmunoprecipitation)
Sonnberg et al., Proc Natl Acad Sci U S A 2008*
-
IRef Dip Interaction:
Complex of CUL1-SKP1
(anti tag coimmunoprecipitation)
Sonnberg et al., Proc Natl Acad Sci U S A 2008*
-
IRef Dip Interaction:
Complex of SKP1-SKP1-CUL1-CUL1
(anti tag coimmunoprecipitation)
Sonnberg et al., Proc Natl Acad Sci U S A 2008*
-
IRef Dip Interaction:
Complex of SKP1-CAND1-CUL1
(anti tag coimmunoprecipitation)
Sonnberg et al., Proc Natl Acad Sci U S A 2008*
-
IRef Dip Interaction:
SKP1
—
CUL1
(physical association, x-ray crystallography)
Zheng et al., Nature 2002*
-
IRef Dip Interaction:
Complex of CUL1-SKP1
(anti tag coimmunoprecipitation)
Sonnberg et al., Proc Natl Acad Sci U S A 2008*
-
IRef Hprd Interaction:
Complex of 26 proteins
(in vivo)
Fukuchi et al., Mol Biol Cell 2001
-
IRef Hprd Interaction:
Complex of 17 proteins
(in vivo)
Gomes et al., Proc Natl Acad Sci U S A 2001*
-
IRef Hprd Interaction:
Complex of CUL1-FBXL3-SKP1-SKP1-FBXL3-CUL1-SKP1-FBXL3-CUL1
(in vivo)
Cenciarelli et al., Curr Biol 1999
-
IRef Hprd Interaction:
Complex of SKP1-CUL1-FBXO15-CUL1-SKP1-FBXO15-FBXO15-SKP1-CUL1
(in vivo)
Tokuzawa et al., Mol Cell Biol 2003*
-
IRef Hprd Interaction:
Complex of 17 proteins
(in vivo)
Yoshida et al., Nature 2002*
-
IRef Hprd Interaction:
Complex of 17 proteins
(in vivo)
Lutz et al., FEBS Lett 2006
-
IRef Hprd Interaction:
Complex of 37 proteins
(in vivo)
Read et al., Mol Cell Biol 2000
-
IRef Hprd Interaction:
Complex of 170 proteins
(in vivo)
Bouwmeester et al., Nat Cell Biol 2004
-
IRef Hprd Interaction:
Complex of 17 proteins
(in vivo)
Lutz et al., FEBS Lett 2006
-
IRef Hprd Interaction:
Complex of 26 proteins
(in vivo)
Suzuki et al., J Biol Chem 2000
-
IRef Hprd Interaction:
Complex of 626 proteins
(in vivo)
Bouwmeester et al., Nat Cell Biol 2004
-
IRef Hprd Interaction:
Complex of CUL1-SKP1-FBXO4-FBXO4-SKP1-CUL1-CUL1-FBXO4-SKP1
(in vivo)
Cenciarelli et al., Curr Biol 1999
-
IRef Hprd Interaction:
SKP1
—
CUL1
(in vitro)
Nemajerova et al., EMBO J 2007*, Ng et al., FEBS Lett 1998*
-
IRef Hprd Interaction:
SKP1
—
CUL1
(in vivo)
Nemajerova et al., EMBO J 2007*, Ng et al., FEBS Lett 1998*
-
IRef Hprd Interaction:
Complex of 19 proteins
(in vivo)
Cenciarelli et al., Curr Biol 1999, Winston et al., Genes Dev 1999
-
IRef Hprd Interaction:
Complex of 33 proteins
(in vivo)
Abida et al., J Biol Chem 2007
-
IRef Hprd Interaction:
Complex of SKP1-CUL1-FBXO7-CUL1-SKP1-FBXO7-FBXO7-SKP1-CUL1
(in vivo)
Cenciarelli et al., Curr Biol 1999
-
IRef Hprd Interaction:
Complex of SKP1-CUL1-CUL1-FBXW2-CUL1-SKP1-SKP1-FBXW2-FBXW2
(in vivo)
Cenciarelli et al., Curr Biol 1999
-
IRef Intact Interaction:
Complex of 11 proteins
(association, anti tag coimmunoprecipitation)
Rodier et al., EMBO J 2008
-
IRef Intact Interaction:
Complex of RRM2-CCNF-CUL1-SKP1
(association, anti tag coimmunoprecipitation)
D'Angiolella et al., Cell 2012
-
IRef Intact Interaction:
Complex of CUL1-CCNF-SKP1
(association, anti tag coimmunoprecipitation)
D'Angiolella et al., Cell 2012
-
IRef Intact Interaction:
Complex of CAND1-FBXO25-SKP1-CUL1
(association, pull down)
Teixeira et al., Proteomics 2010
-
IRef Intact Interaction:
Complex of 35 proteins
(association, anti tag coimmunoprecipitation)
Jäger et al., Nature 2012
-
IRef Intact Interaction:
Complex of 32 proteins
(association, pull down)
Jäger et al., Nature 2012
-
IRef Intact Interaction:
Complex of 35 proteins
(association, anti tag coimmunoprecipitation)
Jäger et al., Nature 2012
-
IRef Intact Interaction:
Complex of FBXL15-CUL1-RBX1-SKP1
(association, anti tag coimmunoprecipitation)
Cui et al., EMBO J 2011
-
IRef Intact Interaction:
Complex of 22 proteins
(physical association, anti tag coimmunoprecipitation)
Sowa et al., Cell 2009
-
IRef Intact Interaction:
Complex of CAND1-CUL1-SKP1-FBXO25-ACTB
(association, pull down)
Teixeira et al., Proteomics 2010
-
IRef Intact Interaction:
Complex of CCDC6-CUL1-SKP1
(association, anti bait coimmunoprecipitation)
Zhao et al., FEBS Lett 2012*
-
IRef Intact Interaction:
Complex of CDK2-RBX1-CDKN1B-SKP1-SKP2-CKS1B-CCNA2-CUL1
(association, molecular sieving)
Hao et al., Mol Cell 2005
-
IRef Intact Interaction:
Complex of SKP1-CUL1-FBXO4
(association, anti tag coimmunoprecipitation)
Hagens et al., Biochim Biophys Acta 2006*
-
IRef Intact Interaction:
Complex of CAND1-SKP1-RBX1-CUL1-BTRC
(association, coimmunoprecipitation)
Min et al., J Biol Chem 2003
-
IRef Intact Interaction:
Complex of RBX1-BTRC-CUL1-UBE2D2-CTNNB1-UBA1-SKP1
(ubiquitination reaction, enzymatic study)
Duda et al., Cell 2008
-
IRef Intact Interaction:
SKP1
—
CUL1
(physical association, anti bait coimmunoprecipitation)
Nemajerova et al., EMBO J 2007*
-
IRef Intact Interaction:
SKP1
—
CUL1
(direct interaction, pull down)
Zhang et al., EMBO J 2008*
-
IRef Intact Interaction:
SKP1
—
CUL1
(direct interaction, isothermal titration calorimetry)
Zhang et al., EMBO J 2008*
-
IRef Intact Interaction:
SKP1
—
CUL1
(physical association, coimmunoprecipitation)
Min et al., J Biol Chem 2003
-
IRef Intact Interaction:
SKP1
—
CUL1
(physical association, anti bait coimmunoprecipitation)
Ganesh et al., Nature 2003
-
IRef Intact Interaction:
Complex of CUL1-SKP2-CUL1-RBX1-SKP1
(physical association, x-ray crystallography)
Zheng et al., Nature 2002*
-
IRef Intact Interaction:
Complex of SKP1-CUL1-BTRC-BTRC-SKP1-CUL1
(association, coimmunoprecipitation)
Min et al., J Biol Chem 2003
-
IRef Intact Interaction:
Complex of SKP1-CAND1-CUL1
(association, anti bait coimmunoprecipitation)
Zheng et al., Mol Cell 2002*
-
IRef Intact Interaction:
Complex of 12 proteins
(association, anti tag coimmunoprecipitation)
Alexandru et al., Cell 2008
-
IRef Intact Interaction:
Complex of SKP1-SKP2-CUL1-SKP1-SKP2-CUL1-SKP1-SKP2-CUL1
(association, coimmunoprecipitation)
Min et al., J Biol Chem 2003
-
IRef Intact Interaction:
Complex of SKP1-RBX1-FBXO11-CUL1-CUL1-SKP1-RBX1-FBXO11
(association, anti tag coimmunoprecipitation)
Abida et al., J Biol Chem 2007
-
IRef Intact Interaction:
Complex of SKP1-CUL1-FBXO7
(association, anti tag coimmunoprecipitation)
Hagens et al., Biochim Biophys Acta 2006*
-
IRef Intact Interaction:
Complex of 12 proteins
(association, anti tag coimmunoprecipitation)
Alexandru et al., Cell 2008
-
IRef Intact Interaction:
Complex of RBX1-CUL1-FBXL12-SKP1
(association, pull down)
Kim et al., EMBO Rep 2008*
-
IRef Intact Interaction:
Complex of CUL1-CDC34-BTRC-SKP1
(physical association, fluorescent resonance energy transfer)
Kleiger et al., Cell 2009*
-
IRef Intact Interaction:
Complex of 23 proteins
(association, anti tag coimmunoprecipitation)
Alexandru et al., Cell 2008
-
IRef Intact Interaction:
Complex of FBXO25-SKP1-CUL1
(association, anti tag coimmunoprecipitation)
Hagens et al., Biochim Biophys Acta 2006*
-
IRef Ophid Interaction:
SKP1
—
CUL1
(aggregation, interologs mapping)
Brown et al., Bioinformatics 2005
Text-mined interactions from Literome
Hatakeyama et al., Proc Natl Acad Sci U S A 1999
:
These results suggest that the substrate-specific degradation of
IkappaBalpha is
mediated by a
Skp1/Cull 1/F-box protein (SCF) FWD1 ubiquitin-ligase complex and that FWD1 serves as an intracellular receptor for phosphorylated IkappaBalpha
Sui et al., Blood 1999
:
The CD34 ( + ) IL-6R- cells produced a number of
Mks as well as Mk colonies in cultures supplemented with sIL-6R/IL-6 or TPO in the
presence of
SCF
Huang et al., Blood 1999
:
However,
interleukin-5 (IL-5) alone sustained survival of TF-1 cells and
required costimulation of
SCF for optimal proliferation ... However,
interleukin-5 (IL-5) alone sustained survival of TF-1 cells and
required costimulation of
SCF for optimal proliferation
Janowska-Wieczorek et al., Blood 1999
(Breast Neoplasms) :
Moreover, the stimulatory
effects of G-CSF and
SCF on both
MMP-2 and MMP-9 secretion were found to be significantly higher in CD34 ( + ) cells isolated from bone marrow than in those from peripheral blood ... Moreover, the stimulatory
effects of G-CSF and
SCF on both MMP-2 and
MMP-9 secretion were found to be significantly higher in CD34 ( + ) cells isolated from bone marrow than in those from peripheral blood
Bao et al., Blood 1999
:
We found that erythropoietin (EPO) and
stem cell factor (SCF) activated
protein kinase B ( PKB/Akt ) in EPO dependent HCD57 erythroid cells
Price et al., Biochem Biophys Res Commun 1999
:
Taken together, these findings show that CHK is able to down-regulate
SCF/KL stimulated
Lyn activity in megakaryocytes
Tsvetkov et al., Curr Biol 1999
:
p27 ( Kip1 ) ubiquitination and degradation is
regulated by the
SCF(Skp2) complex through phosphorylated Thr187 in p27
Mou et al., Biochem J 1999
:
These findings demonstrate that
SCF activates the Src family member
Lyn before the G ( 1 ) /S transition of the cell cycle and suggest that Lyn is involved in SCF induced cell cycle progression
Rouillon et al., EMBO J 2000
:
Feedback regulated degradation of the transcriptional activator
Met4 is
triggered by the
SCF ( Met30 ) complex
Jiang et al., Blood 2000
:
In contrast, cyclin-E and
p27 ( KIP1 ) protein levels and cdk2 activity in cells adherent to FN in the
presence of IL3 or
SCF were similar to those in PLL-adherent and FN-nonadherent or PLL-nonadherent cells ... In contrast,
cyclin-E and p27 ( KIP1 ) protein levels and cdk2 activity in cells adherent to FN in the
presence of IL3 or
SCF were similar to those in PLL-adherent and FN-nonadherent or PLL-nonadherent cells
Blume-Jensen et al., Nat Genet 2000
:
The mutation completely disrupted PI 3'-kinase binding to Kit/SCF-R and reduced
SCF induced PI 3'-kinase dependent activation of
Akt by 90 %
Meimoun et al., Mol Biol Cell 2000
:
Here we report that
SCF ( CDC4 ), a recently characterized protein complex that acts in conjunction with the ubiquitin conjugating enzyme Cdc34 to degrade cell cycle regulators, is also
necessary for the degradation of the
transcription factor Gcn4. Degradation of Gcn4 occurs throughout the cell cycle, whereas degradation of the known cell cycle substrates of Cdc34/SCF(CDC4) is cell cycle regulated
Read et al., Mol Cell Biol 2000
:
The site of Nedd8 ligation to Cul-1 is essential, as SCF(beta(TrCP)) containing a K720R mutant of Cul-1 only weakly supported IkappaBalpha ubiquitination compared to SCF(beta(TrCP)) containing WT Cul-1, suggesting that the Nedd8 ligation of
Cul-1 affects the ubiquitination activity of
SCF(beta(TrCP)) ... The site of Nedd8 ligation to Cul-1 is essential, as SCF(beta(TrCP)) containing a K720R mutant of Cul-1 only weakly supported IkappaBalpha ubiquitination compared to SCF(beta(TrCP)) containing WT Cul-1, suggesting that the
Nedd8 ligation of Cul-1
affects the ubiquitination activity of
SCF(beta(TrCP))
Feng et al., J Biol Chem 2000
:
SCF induced
cyclin D3 expression and phosphorylation of the retinoblastoma protein through a pathway that is sensitive to both wortmannin and rapamycin
Bondzi et al., Cell Growth Differ 2000
(Carcinoma, Small Cell...) :
In the present study, we have demonstrated that PP1, a pharmacological inhibitor of Src kinases, blocked
SCF mediated
activation of
mitogen activated protein ( MAP ) kinase , but it also inhibited Kit activation
Sui et al., Br J Haematol 2000
:
We have recently demonstrated that
SCF and EPO synergistically
activate mitogen activated protein ( MAP ) kinase , thereby promoting growth of human erythroid colony forming cells ( ECFCs )
Krystal et al., Clin Cancer Res 2000
(Carcinoma, Small Cell...) :
STI571 efficiently blocked
SCF mediated activation of
mitogen activated protein kinase and Akt, but did not affect insulin-like growth factor-1 or serum mediated mitogen activated protein kinase or Akt activation ... STI571 efficiently blocked
SCF mediated activation of mitogen activated protein kinase and
Akt , but did not affect insulin-like growth factor-1 or serum mediated mitogen activated protein kinase or Akt activation
Erickson-Miller et al., Stem Cells 2000
(Leukemia, Erythroblastic, Acute) :
The
activation of
MAPK ( p42/44 ) by
SCF was transient, lasting only 30 min, in contrast to Epo, which stimulated phosphorylation of p42/44 for up to 2 h ... The expression of the early response genes c-fos, egr1, and
cytokine-inducible SH2 protein (CIS) in
response to
SCF or Epo stimulation demonstrated that the transient expression of p42/44 correlated with the transient expression of c-fos and egr1 ... The expression of the early response genes c-fos,
egr1 , and cytokine-inducible SH2 protein (CIS) in
response to
SCF or Epo stimulation demonstrated that the transient expression of p42/44 correlated with the transient expression of c-fos and egr1 ... In addition,
CIS was
activated by Epo but not
SCF
Bone-Larson et al., Am J Pathol 2000
(Acute Disease...) :
Immunoneutralization studies revealed that the
SCF stimulated release of
monocyte chemoattractant protein-1 ( MCP-1 ) into the septic peritoneal cavity contributed to the protective effect of SCF in this model
Smothers et al., Mol Cell Biol 2000
:
Here we demonstrate that in vivo
SCF ( Met30p ) complex activity can be
regulated by the abundance of
Met30p
Ema et al., J Exp Med 2000
:
In the
presence of
stem cell factor (SCF) ,
thrombopoietin (TPO) induced a first cell division of CD34 ( - ) KSL cells more efficiently than did interleukin (IL)-3 or IL-6
Duarte et al., Blood 2000
:
However,
SCF induces phosphorylation of
STAT3 on serine727 ( ser727 ), which is necessary for maximal STAT transcriptional activity, and the combination of SCF and G-CSF leads to complete STAT3 phosphorylation on ser727 ... The pathways by which
SCF and G-CSF
lead to serine phosphorylation of
STAT3 are distinct and are partially dependent on phosphatidylinositol-3 kinase and ERKs, pathways that are also necessary for the synergistic effects of SCF and G-CSF on proliferation and c-fos induction
Nishio et al., Exp Hematol 2001
:
The
Fas ligand (Fas-L) expressed on mature erythroblasts may
induce apoptosis of more immature erythroid cells that express Fas, whereas
stem cell factor (SCF) may prevent Fas mediated cell death in hematopoietic progenitor cells ...
SCF also
inhibited the activation of
caspase-3 and caspase-8, without downregulation of the surface expression of Fas, suggesting that SCF prevents apoptosis through uncoupling of Fas ligation from subsequent caspase activation
Matsui et al., Int J Hematol 2000
:
We have reported that simultaneous
activation of
glycoprotein (gp) 130 and c-kit signals by interleukin (IL)-6, soluble IL-6 receptor ( sIL-6R ), and
stem cell factor (SCF) promotes proliferation of human hematopoietic progenitor cells and their differentiation into erythroid, myelocytic, and megakaryocytic cells
Tanaka et al., Biochimie 2001
:
In this NF-kappaB signalling pathway, it becomes clear that the
SCF ( Fbw1 ) activity is
enhanced by a
ubiquitin-like protein NEDD8 ( equivalent to Rub1 ) that modifies Cul-1 in a manner analogous to ubiquitylation, and consequently, IkappaBalpha proteolysis is induced
Ito et al., Fertil Steril 2001
:
HGF increased the expression of
SCF gene in granulosa cells, and
SCF reciprocally
increased the expression of HGF gene in theca cells ...
HGF increased the expression of SCF gene in granulosa cells, and
SCF reciprocally
increased the expression of HGF gene in theca cells
Oliveira et al., Inflamm Res 2001
:
In the present study we investigated the
effect of
SCF and/or IgE on histamine,
TNF-alpha and chemokines released from bone marrow derived mast cells ( BMMC ) as well as chemokine receptor expression
Yamamoto et al., J Dermatol Sci 2001
(Fibrosis) :
Here, we examined the
effect of
stem cell factor (SCF) , a mast cell growth factor, on
MCP-1 gene expression in a human mast cell line, HMC-1, and as well as the effect of MCP-1 on alpha1 ( I ) collagen gene expression in human skin fibroblasts ... Stimulation with SCF further upregulated
MCP-1 mRNA expression in a time- and dose dependent manner, and stimulation with 100 ng/ml
SCF for 24 h
induced a 3-fold increase of MCP-1 mRNA expression in HMC-1 cells as compared with unstimulated cells
Plo et al., Oncogene 2001
:
In Ba/F3-wt and in Ba/F3-Kit cells, daunorubicin stimulated within 8-15 min neutral sphingomyelinase and ceramide production but not in
SCF stimulated
Ba/F3-Kit or in Ba/F3-KitDelta27 whereas all Ba/F3 cells were equally sensitive to exogenous cell-permeant C6-ceramide
Angchaisuksiri et al., Blood 2002
:
However, medium conditioned by IL-2 stimulated, but not resting, NK cells ( NKCM ) contained a trypsin-sensitive factor capable of increasing 2- to 5-fold the number of polyploid megakaryocytes generated in vitro from IL-3 and
SCF stimulated
CD34 ( + ) cells
Bovolenta et al., Blood 2002
(Leukemia, Erythroblastic, Acute) :
Neutralization experiments indicated that GM-CSF and IFN-gamma, but not
SCF , were
responsible for HTLV-2 induced
STAT activation, whereas anti-GM-CSF antibodies greatly inhibited TF-1 cell proliferation
Prignano et al., J Eur Acad Dermatol Venereol 2001
:
Moreover, TNF-alpha induced expression of CD54 by cells in the medium, but not by those retained in the sheets, whereas human
SCF induced , dose dependently, expression of
CD54 by cells in the sheets, but not from those in the medium ... Moreover,
TNF-alpha induced expression of CD54 by cells in the medium, but not by those retained in the sheets, whereas human
SCF induced, dose dependently, expression of CD54 by cells in the sheets, but not from those in the medium
Gyotoku et al., Arch Dermatol Res 2001
:
Some
IL-6 family cytokines
enhanced the production of
stem cell factor (SCF) , a potent mast cell growth factor, from NIH/3T3 fibroblasts, but the amount of SCF produced by NIH/3T3 fibroblasts was not paralleled by the mast cell growth-enhancement induced by the IL-6 family cytokines
Sitry et al., J Biol Chem 2002
:
Previous studies have shown that the cyclin dependent kinase (Cdk) inhibitor p27 ( Kip1 ) is targeted for degradation by an
SCF ( Skp2 ) ubiquitin ligase complex and that this process
requires Cks1 , a member of the highly conserved Suc1/Cks family of cell cycle regulatory proteins
Maddens et al., Blood 2002
:
Moreover,
SCF stimulation
inhibited IR-induced
neutral sphingomyelinase (N-SMase) stimulation and ceramide production
Fujiki et al., Exp Hematol 2002
:
Moreover, the endogenous production of
TPO by cultured CD34 ( + ) IL-6R ( - ) cells in the
presence of
Epo+SCF+IL-9 was ruled out by reverse transcriptase polymerase chain reaction for TPO mRNA
Mendoza et al., J Biol Chem 2003
:
The
ubiquitin-like protein NEDD8 is
essential for activity of
SCF-like ubiquitin ligase complexes
Pintard et al., Curr Biol 2003
:
Conjugation of the
ubiquitin-like protein Nedd8 to the cullin subunit ( neddylation ) positively
regulates activity of
SCF complexes, most likely by increasing their affinity for the E2 conjugated to ubiquitin
Boer et al., Exp Hematol 2003
:
STAT5 activation was examined in erythroid cell lines by analyzing the
effects of EPO and
SCF on
STAT5 tyrosine phosphorylation, serine phosphorylation, DNA binding, and STAT5 mediated gene transactivation ... SCF pretreatment followed by EPO stimulation resulted in a 9.0-fold+/-0.9-fold increase in STAT5 transactivation, while
SCF alone did not
increase STAT5 transactivation ...
SCF enhances the EPO mediated
STAT5 transactivation by triggering a PKA/CREB dependent pathway
Yuan et al., Zhonghua Nan Ke Xue 2003
:
It was reported that
SCF/c-kit , Bcl-2 and Bcl-xl inhibited the apoptosis while caspase-3,
Fas , Bax and clusterine
stimulated it ... It was reported that
SCF/c-kit , Bcl-2 and Bcl-xl inhibited the apoptosis while caspase-3, Fas,
Bax and clusterine
stimulated it
Kamura et al., Proc Natl Acad Sci U S A 2003
:
Finally, the purified recombinant
SCFSkp2 complex mediated
p57Kip2 ubiquitylation in vitro in a manner dependent on the presence of the cyclin E-CDK2 complex
Jeong et al., Endocrinology 2003
:
Our results show that dexamethasone potently regulates
SCF induced migration,
p38 MAPK activation, and inflammatory cytokine production through the expression of MKP-1 protein in RPMCs ... Our results show that dexamethasone potently regulates
SCF induced migration, p38
MAPK activation, and inflammatory cytokine production through the expression of MKP-1 protein in RPMCs
Cope et al., Cell 2003
:
Here, we summarize what is known about CSN, and discuss hypotheses for how
CSN promotes the activity of
SCF ubiquitin ligases
Kumar et al., EMBO J 2003
:
SCF ( HOS ) ubiquitin ligase
mediates the ligand induced down-regulation of the
interferon-alpha receptor
Yu et al., Science 2003
:
Induction of
APOBEC3G ubiquitination and degradation by an HIV-1
Vif-Cul5-SCF complex
Ren et al., J Clin Invest 2003
:
In vitro studies using primary mouse hepatocytes demonstrate that
SCF causes hepatocyte proliferation and is
induced by
IL-6 and that treatment with anti-SCF antibodies inhibits IL-6 induced hepatocyte proliferation
Bayle et al., J Biol Chem 2004
:
Ectopic expression of
SOCS6 in Ba/F3-KIT cell line decreased cell proliferation in
response to
SCF but not SCF induced chemotaxis ... SOCS6 reduced
SCF induced activation of
ERK1/2 and p38 but not activation of AKT or STATs in Ba/F3, murine embryonic fibroblast (MEF), or COS-7 cells ... SOCS6 reduced
SCF induced activation of ERK1/2 and p38 but not activation of
AKT or STATs in Ba/F3, murine embryonic fibroblast (MEF), or COS-7 cells ... SOCS6 reduced
SCF induced
activation of ERK1/2 and
p38 but not activation of AKT or STATs in Ba/F3, murine embryonic fibroblast (MEF), or COS-7 cells
Yamano et al., J Biol Chem 2004
:
Here we show that fission yeast S phase cyclin
Cig2 is ubiquitylated and degraded via both the SCF and the APC/C. Cig2 instability during G ( 2 ) and M phase is
dependent upon the
SCF complex , whereas the APC/C is responsible for Cig2 destruction during anaphase and G ( 1 ), thereby ensuring a spike pattern of Cig2 levels, peaking only at S phase ...
SCF ( Pop1/Pop2 ) therefore
regulates Cig2 levels in a dual manner, transcriptionally and post-translationally
Wan et al., J Biol Chem 2004
:
Smad4 protein stability is
regulated by ubiquitin ligase
SCF beta-TrCP1
Kurland et al., Cancer Cell 2004
:
Crashing waves of destruction : the cell cycle and
APC ( Cdh1 )
regulation of
SCF ( Skp2 )
Kondo et al., J Biol Chem 2004
:
Rapid degradation of
Cdt1 upon UV-induced DNA damage is
mediated by
SCFSkp2 complex
Carrabino et al., Brain Res Gene Expr Patterns 2004
:
The
CSN controls the function of the
SCF ubiquitin-ligase complex through an enzymatic activity that removes the small ubiquitin-like molecule NEDD8 from the cullin component of the SCF and that requires subunit 5 of the CSN ( JAB1/CSN5 )
Zeng et al., Blood 2005
:
We propose a negative feedback loop controlling the SCF-KIT signaling pathway, in which
SCF activates
KIT
Scholl et al., J Cancer Res Clin Oncol 2004
(Leukemia, Myeloid) :
Stimulation with
SCF leads to a significant increase in interaction between Kit and
p85 as well as in receptor associated PI 3-kinase activity ... Stimulation with
SCF leads to a significant increase in interaction between Kit and p85 as well as in receptor associated
PI 3-kinase activity
Dai et al., Exp Hematol 2004
:
The activations of caspase 3 and
caspase 8 were almost completely
blocked by
SCF while CDK6 and the FLICE-inhibitory protein (FLIP) were increased
Sangrar et al., Exp Hematol 2004
(Anemia) :
Fps/Fes was also activated in
response to erythropoietin (EPO) and
stem cell factor (SCF) , two critical factors in erythroid development ...
Fps/Fes was also activated in
response to erythropoietin (EPO) and
stem cell factor (SCF) , two critical factors in erythroid development
Jin et al., Mol Reprod Dev 2005
:
While
SCF up-regulated the expression of the anti-apoptotic proteins Bcl-2 and
Bcl-xL , it did the opposite to the pro-apoptotic factor Bax ... While
SCF up-regulated the expression of the anti-apoptotic proteins
Bcl-2 and Bcl-xL, it did the opposite to the pro-apoptotic factor Bax
Hue et al., Immunol Lett 2005
(Melanoma, Experimental) :
IL-18 enhances
SCF production of melanoma cells by regulating ROI and p38 MAPK activity ... Furthermore, inhibitors of
p38 mitogen activated protein kinase ( MAPK ), such as SB203580,
blocked enhanced
SCF expression, indicating that p38 MAPK activity is required for IL-18 enhanced SCF production
Kohl et al., Blood 2005
(Acute Disease...) :
All
KIT exon 8 mutants induced receptor hyperactivation in
response to
stem cell factor (SCF) stimulation in terms of proliferation and resistance toward apoptotic cell death
Barbey et al., EMBO J 2005
:
Cd2+ inhibits
SCF ( Met30 ) activity through rapid dissociation of the F-box protein Met30 from the holocomplex
Drayer et al., Stem Cells 2005
:
TPO stimulation of MO7e cells resulted in STAT5 transactivation, which could be enhanced 1.6-fold by costimulation with SCF, whereas
SCF alone did not
induce STAT5 transcriptional activity ...
SCF induced enhancement of
JAK2 phosphorylation was not affected by inhibition of Src kinase, suggesting that both JAK2 and Src kinase mediate STAT5 tyrosine phosphorylation
Tsang et al., J Leukoc Biol 2005
(Inflammation...) :
Synergistic
effect of
SCF and TNF-alpha on the up-regulation of cell-surface expression of
ICAM-1 on human leukemic mast cell line ( HMC ) -1 cells ... A synergistic
effect of
SCF and TNF-alpha on
ICAM-1 expression was demonstrated ... Results indicated that
SCF activated ERK, and
TNF-alpha activated the p38 MAPK and NF-kappaB pathway ... Results indicated that
SCF activated
ERK , and TNF-alpha activated the p38 MAPK and NF-kappaB pathway ... Therefore,
SCF and TNF-alpha
acted through
ERK and the NF-kappaB pathway to regulate the ICAM-1 expression and elicited the synergistic effect ... Therefore,
SCF and TNF-alpha acted through ERK and the NF-kappaB pathway to
regulate the
ICAM-1 expression and elicited the synergistic effect
Dreier et al., Neuron 2005
:
Instead,
SCF ( LIN-23 )
regulates GLR-1 through the beta-catenin homolog BAR-1 and the TCF/Lef transcription factor homolog POP-1
Möller et al., Blood 2005
:
SCF induced a rapid and transient phosphorylation of Akt ( protein kinase B ) and
FOXO3a ...
SCF induced a rapid and transient phosphorylation of
Akt ( protein kinase B ) and FOXO3a
Wan et al., Am J Pathol 2005
(Adenocarcinoma...) :
SCF(beta-TrCP1) controls
Smad4 protein stability in pancreatic cancer cells
Zhang et al., Zhongguo Shi Yan Xue Ye Xue Za Zhi 2005
:
This study was aimed to evaluate the
effect of FL,
SCF and TPO on
CD34 ( + ) expansion
Benmaamar et al., Cell cycle (Georgetown, Tex.) 2005
:
Involvement of the
SCF complex in the control of
Cdh1 degradation in S-phase
Hassel et al., J Cell Physiol 2006
:
Abrogation of the
c-kit/SCF dependent phosphorylation of
BMPR-II at the Ser757 interfered with the cooperative effect of BMP2 and SCF
Blázquez-Domingo et al., Mol Cell Biol 2005
(Cell Transformation, Neoplastic) :
SCF induced
phosphoinositide-3-kinase (PI3K) appeared to control polysome recruitment of specific mRNAs associated with neoplastic transformation
Khanzada et al., Oncogene 2006
(Carcinoma, Small Cell...) :
These responses correlated with the inhibition of
stem cell factor (SCF) stimulated activation of extracellular signal regulated kinase (
Erk ), protein kinase B (PKB) and ribosomal S6 kinase by simvastatin ... These responses correlated with the inhibition of
stem cell factor (SCF) stimulated activation of extracellular signal regulated kinase ( Erk ),
protein kinase B (PKB) and ribosomal S6 kinase by simvastatin
Dang et al., J Neurosci Res 2005
(Neurofibromatoses) :
Although the c-Kit ligand
stem cell factor (SCF) induces the phosphorylation of protein kinase B ( or
Akt ) and prevents apoptosis in Schwann cells, SCF has no effect on the proliferation or differentiation of Schwann cells
Kuhlmann et al., J Exp Med 2006
(Arrhythmias, Cardiac...) :
G-CSF/SCF increased cardiomyocyte diameter, arteriogenesis, and expression of
connexin43 in the border zone of the infarction
Bouchelouche et al., J Urol 2006
:
Furthermore, IL-1beta and tumor necrosis factor-alpha alone induced significant release of MCP-1, IL-6 and
SCF but in combination with IL-4 or IL-13 it
induced greater secretion of
MCP-1 , IL-6 and SCF ... Furthermore, IL-1beta and
tumor necrosis factor-alpha alone
induced significant release of MCP-1, IL-6 and
SCF but in combination with IL-4 or IL-13 it induced greater secretion of MCP-1, IL-6 and SCF ... Furthermore,
IL-1beta and tumor necrosis factor-alpha alone
induced significant release of MCP-1, IL-6 and
SCF but in combination with IL-4 or IL-13 it induced greater secretion of MCP-1, IL-6 and SCF ... Furthermore,
IL-1beta and tumor necrosis factor-alpha alone
induced significant release of MCP-1, IL-6 and
SCF but in combination with IL-4 or IL-13 it induced greater secretion of MCP-1, IL-6 and SCF ... Furthermore, IL-1beta and tumor necrosis factor-alpha alone induced significant release of MCP-1, IL-6 and
SCF but in combination with IL-4 or IL-13 it
induced greater secretion of MCP-1,
IL-6 and SCF ... Furthermore, IL-1beta and tumor necrosis factor-alpha alone induced significant release of MCP-1, IL-6 and
SCF but in combination with IL-4 or IL-13 it
induced greater secretion of MCP-1, IL-6 and
SCF
Lin et al., Cell cycle (Georgetown, Tex.) 2006
:
Importantly, our studies demonstrate that
SCF ( xSkp2 ) supports the ubiquitination of Xic1 in a reconstituted in vitro ubiquitination assay and that this Xic1 ubiquitination does not
require either
CDK2-cyclins or Cks1 ... Importantly, our studies demonstrate that
SCF ( xSkp2 ) supports the ubiquitination of Xic1 in a reconstituted in vitro ubiquitination assay and that this Xic1 ubiquitination does not
require either CDK2-cyclins or
Cks1
Wong et al., Allergy 2006
:
Either
SCF or TNF-alpha could
induce release from HMC-1 cells of interleukin (IL)-8, monocyte chemoattractant protein (MCP)-1, regulated upon activation normal T-cell expressed and secreted ( RANTES ), and I-309, while SCF and TNF-alpha induced release of macrophage inflammatory protein (MIP)-1beta and
interferon-gamma-inducible protein-10 (IP-10) , respectively ... Either
SCF or TNF-alpha could
induce release from HMC-1 cells of interleukin (IL)-8, monocyte chemoattractant protein (MCP)-1, regulated upon activation normal T-cell expressed and secreted ( RANTES ), and I-309, while SCF and
TNF-alpha induced release of macrophage inflammatory protein (MIP)-1beta and interferon-gamma-inducible protein-10 (IP-10), respectively ... Either
SCF or TNF-alpha could
induce release from HMC-1 cells of interleukin (IL)-8, monocyte chemoattractant protein (MCP)-1, regulated upon activation normal T-cell expressed and secreted ( RANTES ), and I-309, while SCF and TNF-alpha induced release of macrophage inflammatory protein
(MIP)-1beta and interferon-gamma-inducible protein-10 (IP-10), respectively ... Either
SCF or TNF-alpha could
induce release from HMC-1 cells of interleukin (IL)-8, monocyte chemoattractant protein (MCP)-1, regulated upon activation normal T-cell expressed and secreted ( RANTES ), and I-309, while SCF and TNF-alpha induced release of macrophage inflammatory protein (MIP)-1beta and
interferon-gamma-inducible protein-10 (IP-10), respectively ... Either
SCF or TNF-alpha could
induce release from HMC-1 cells of interleukin (IL)-8, monocyte chemoattractant protein (MCP)-1, regulated upon activation normal T-cell expressed and secreted ( RANTES ), and I-309, while
SCF and TNF-alpha induced release of macrophage inflammatory protein (MIP)-1beta and interferon-gamma-inducible protein-10 (IP-10), respectively ... Either
SCF or TNF-alpha could
induce release from HMC-1 cells of interleukin (IL)-8, monocyte chemoattractant protein (MCP)-1, regulated upon activation normal T-cell expressed and secreted ( RANTES ), and I-309, while SCF and TNF-alpha induced release of
macrophage inflammatory protein (MIP)-1beta and interferon-gamma-inducible protein-10 (IP-10), respectively ... The induction of RANTES by
SCF or TNF-alpha was mediated by ERK and NF-kappaB, respectively, and SCF
induced MIP-1beta release was mediated by ERK ... The induction of RANTES by SCF or
TNF-alpha was mediated by ERK and NF-kappaB, respectively, and
SCF induced MIP-1beta release was mediated by ERK ... The induction of RANTES by SCF or TNF-alpha was mediated by
ERK and NF-kappaB, respectively, and
SCF induced MIP-1beta release was mediated by ERK ... The induction of RANTES by SCF or TNF-alpha was mediated by ERK and
NF-kappaB , respectively, and
SCF induced MIP-1beta release was mediated by ERK
De Schepper et al., Arch Dermatol Res 2006
(Cafe-au-Lait Spots...) :
In this study we compared epidermal melanocyte and dermal mast cell numbers between four groups : control normal and control CALM skin, and NF1 normal and NF1 CALM skin and elaborated a possible
role for
stem cell factor (SCF) in
CALM formation
Yoshikubo et al., Exp Hematol 2006
:
Human UCB CD34 ( + ) cells were stably expanded on MS-5 stroma cells in the
presence of
SCF , FL, and
TPO
Angers et al., Nat Cell Biol 2006
:
Considered with evidence that the distinct Cullin-1 based
SCF(beta-TrCP)complex regulates
beta-catenin stability, our data on the stability of Dishevelled demonstrates that two distinct ubiquitin ligase complexes regulate the Wnt-beta-catenin pathway
Mao et al., Zhongguo Shi Yan Xue Ye Xue Za Zhi 2006
:
SCF + FL + TPO combination not only promotes progenitor cells expansion but also
upregulates the expression of CD49d and
CXCR4 on CD34+ cells from cord blood ...
SCF + FL + TPO combination not only promotes progenitor cells expansion but also
upregulates the expression of
CD49d and CXCR4 on CD34+ cells from cord blood
Liu et al., Biochem Biophys Res Commun 2006
:
We hypothesize that
CENP-E degradation is essential for faithful mitotic exit and the proteolysis of CENP-E is
mediated by
SCF via a direct Skp1 link
Noubissi et al., Nature 2006
:
Overexpression of
CRD-BP stabilizes betaTrCP1 mRNA and elevates betaTrCP1 levels ( both in cells and in vivo ),
resulting in the activation of the
Skp1-Cullin1-F-box protein (SCF) ( betaTrCP ) E3 ubiquitin ligase and in accelerated turnover of its substrates including IkappaB and beta-catenin ... Overexpression of
CRD-BP stabilizes betaTrCP1 mRNA and elevates betaTrCP1 levels ( both in cells and in vivo ),
resulting in the activation of the Skp1-Cullin1-F-box protein
(SCF) ( betaTrCP ) E3 ubiquitin ligase and in accelerated turnover of its substrates including IkappaB and beta-catenin
van Drogen et al., Mol Cell 2006
:
SCF ( hCdc4alpha ) binds a complex containing cyclin E, Cdk2, and the prolyl cis/trans isomerase Pin1 and
promotes the activity of
Pin1 without directly ubiquitylating cyclin E
Litz et al., Mol Cancer Ther 2006
(Carcinoma, Small Cell...) :
In addition to hypoxia, peptide growth factors are known to regulate VEGF expression but the
effect of
stem cell factor (SCF) , the ligand for c-Kit, on
VEGF expression has not been characterized ...
SCF increased nuclear levels of the HIF-1alpha
transcription factor , which correlated well with increased HIF-1alpha binding to a consensus hypoxia-responsive element ... These data indicate that activation of c-Kit by
SCF leads to a predominantly HIF-1alpha mediated enhancement of
VEGF expression and that inhibition of c-Kit signaling with imatinib could result in inhibition of tumor angiogenesis
Halfter et al., Cancer Res 2006
(Glioblastoma) :
Degradation of
p27 in late G ( 1 ) and S phase is
initiated by the ubiquitin ligase complex
SCF-Skp2/Cks1
Peschiaroli et al., Mol Cell 2006
:
SCFbetaTrCP mediated degradation of
Claspin regulates recovery from the DNA replication checkpoint response ... Thus, the
SCFbetaTrCP dependent degradation of
Claspin is necessary for the efficient and timely termination of the DNA replication checkpoint
Yao et al., J Mol Biol 2006
:
Activation of ubiquitin ligase
SCF ( Skp2 ) by
Cks1 : insights from hydrogen exchange mass spectrometry
Megy et al., FEBS Lett 2006
(Neoplasms) :
Phosphorylation of
beta-Catenin at sites Ser33 and Ser37 on the DSGXXS motif is
required for the interaction of beta-Catenin with the ubiquitin ligase
SCF(beta-TrCP)
Zielke et al., EMBO Rep 2006
:
We propose that
Rca1 acts as an F-box protein in an as yet uncharacterized
SCF complex , which promotes S-phase entry
Katiyar et al., Mol Cell Biol 2007
:
Mutation of the c-Jun binding site abolished
c-Jun mediated
induction of the
SCF promoter
Dolgachev et al., J Leukoc Biol 2007
:
The data in the present study outline the signal transduction events that are induced by SCF in eosinophils and further demonstrate that MEK mediated signaling pathways are crucial for
SCF induced
CCL6 chemokine activation and eosinophil survival ... Subsequent analysis of CCL6 gene activation and production induced by SCF in the presence or absence of rather specific inhibitors for certain pathways demonstrated that the MEK/MAPK pathway but not the PI-3K pathway was crucial for the
SCF induced
CCL6 gene activation
Arcaro et al., Cell Signal 2007
(Carcinoma, Small Cell) :
Lipid raft integrity was essential for
Src activation in
response to
stem cell factor (SCF) and raft disruption selectively inhibited activation of protein kinase B (PKB)/Akt in response to SCF stimulation
Kim et al., Toxicol Appl Pharmacol 2007
:
We also showed that SC-236 suppresses the
SCF induced
p38 MAPK
activation in RPMCs ... We also showed that SC-236 suppresses the
SCF induced p38
MAPK activation in RPMCs
Yasuda et al., Dig Dis Sci 2007
(Colorectal Neoplasms...) :
In KIT positive lines,
KIT was
activated by
stem cell factor (SCF)
Kondo et al., Blood 2007
:
Moreover, KIT D816V and
stem cell factor (SCF) activated wild-type
KIT were found to induce Hsp32 promoter activity, expression of Hsp32 mRNA, and expression of the Hsp32 protein in Ba/F3 cells
van Kerkhof et al., J Biol Chem 2007
:
The ubiquitin ligase
SCF(betaTrCP) regulates the degradation of the
growth hormone receptor
Zeuner et al., Cancer Res 2007
(Thrombocytopenia) :
In chemotherapy treated megakaryocytes,
SCF activated Akt, neutralized the mitochondrial apoptotic machinery, and
inhibited caspase activity ... In chemotherapy treated megakaryocytes,
SCF activated
Akt , neutralized the mitochondrial apoptotic machinery, and inhibited caspase activity
Navarro et al., Anticancer Res 2007
(Bone Neoplasms...) :
High expression of
c-kit/SCF ( 2+, 3+ ) was
detected in 28/45 cases of EFT ( 62.2 % ), whereas
FAS-FAS-L and IGF-IR were observed in 16/45 ( 37.7 % ) and 9/45 ( 20 % ), respectively ... High expression of
c-kit/SCF ( 2+, 3+ ) was
detected in 28/45 cases of EFT ( 62.2 % ), whereas FAS-FAS-L and
IGF-IR were observed in 16/45 ( 37.7 % ) and 9/45 ( 20 % ), respectively
Djouder et al., Mol Cell 2007
:
Growth factor stimulation induces disassembly of URI/PP1gamma complexes through
S6K1 mediated phosphorylation of
URI at serine 371
Setoyama et al., Proc Natl Acad Sci U S A 2007
:
We propose that Cdc2 and Plx1 sequentially phosphorylate
CPEB and target it for
SCF(beta-TrCP) dependent degradation in Xenopus oocytes
Murphy et al., J Immunol 2007
:
Activation of PKD1 was inhibited by the combined
PKD1 and protein kinase C ( PKC ) inhibitor Gö 6976 but not by broad-spectrum PKC inhibitors, including bisindolylmaleimide ( Bim ) I. Pam ( 3 ) CSK ( 4 ) and
SCF also
induced phosphorylation of heat shock protein 27, a known substrate of PKD1, which was also inhibited by Gö 6976 but not Bim I in BMMC
Kasamatsu et al., J Invest Dermatol 2008
:
It has been demonstrated in other systems that a soluble form of
m-KIT released from the cell surface ( s-KIT )
regulates SCF signaling, although there have been no reports pertaining to the existence and the biological role of s-KIT in melanocytes
Revai et al., Pediatr Infect Dis J 2008
(Hearing Disorders...) :
Upper respiratory tract infections (URI) likely
lead to
acute otitis media (AOM) by causing Eustachian tube dysfunction which creates negative middle ear pressure
Han et al., Carcinogenesis 2008
(Neovascularization, Pathologic) :
Hypoxia and epidermal growth factor receptor signal coexisted in the tumor microenvironment and might promote angiogenesis through
HIF-1 alpha mediated upregulation of
SCF and other angiogenic factors
Whitehurst et al., Mol Cell Biol 2008
:
Surprisingly, we found that
RASSF1A is also
required to restrict
SCF(betaTrCP) activity to allow G/S phase transition
Seki et al., J Cell Biol 2008
:
Proteolysis of Bora requires the
Plk1 kinase activity and is
mediated by
SCF-beta-TrCP
Hu et al., Am J Physiol Gastrointest Liver Physiol 2008
(Drug-Induced Liver Injury) :
SCF inhibited APAP induced hepatocyte apoptosis and increased Bcl-2 and
Bcl-xL expression, suggesting that this decrease in hepatocyte apoptosis is mediated through Bcl-2 and Bcl-xL ...
SCF inhibited APAP induced hepatocyte apoptosis and increased
Bcl-2 and Bcl-xL expression, suggesting that this decrease in hepatocyte apoptosis is mediated through Bcl-2 and Bcl-xL
Zhang et al., Proc Natl Acad Sci U S A 2008
:
The fact that both decreased and increased
CAND1-CUL1 interactions
result in reduced
SCF ( TIR1 ) activity in vivo strongly supports the hypothesis that CAND1 mediated cycling is required for optimal SCF function ... The fact that both decreased and increased
CAND1-CUL1 interactions
result in reduced
SCF ( TIR1 ) activity in vivo strongly supports the hypothesis that CAND1 mediated cycling is required for optimal SCF function
Li et al., J Immunotoxicol 2008
:
To examine if Egr-1 is required for
SCF induced
IL-13 expression, Egr-1-deficient BMMC were used
Moon et al., Free Radic Biol Med 2008
:
Exogenous NO augmented COX-2 protein expression and increased COX-2 dependent
PGD ( 2 ) generation in
response to
SCF , IL-10, and IL-1beta, or antigen activation in combination with IL-10 and IL-1beta after sensitization with IgE
Ang et al., J Biol Chem 2008
:
In the
presence of
Plk2 , SPAR physically associated with the
SCF(beta-TRCP) complex through a canonical phosphodegron
Pedersen et al., Biochem Biophys Res Commun 2008
(Neoplasms...) :
In addition,
SCF induced
HIF-1alpha was transcriptionally active, and transcribed HIF-1 target genes such as VEGF, BNIP3, GLUT1 and DEC1, an effect that could be reversed by siRNA against HIF-1alpha
Jeon et al., Pigment Cell Melanoma Res 2009
:
Here, we examined whether
SCF induces
ezrin/radixin/moesin (ERM) proteins phosphorylation as a downstream target of PI3K in melanocytes
Cen et al., J Biol Chem 2009
:
Taken together, our findings indicate that HINT1 up-regulates cellular levels of p27 ( KIP1 ) by two mechanisms : 1 ) it inhibits its ubiquitylation by targeting the
SCF ( SKP2 ) ubiquitin ligase complex, and 2 ) it
inhibits the phosphorylation of
p27 ( KIP1 ) by Src via inhibiting Src expression
Dolgachev et al., Am J Pathol 2009
(Lung Diseases) :
Interestingly,
SCF stimulated
interleukin-31 receptor expression in bone marrow cells, whereas interleukin-31 strongly induced telomerase reverse transcriptase expression in fibroblasts
Reber et al., PloS one 2009
(Inflammation) :
Ser276 phosphorylation of NF-kB
p65 by MSK1
controls SCF expression in inflammation ... Finally, we demonstrate that both transfections of MSK1-KD and MSK1 siRNA -- but not the WT MSK1 or control siRNA -- downregulate the expression of
SCF induced by
IL-1ss
Lin et al., Nat Cell Biol 2009
(Neoplasm Metastasis...) :
We find that
Skp2 phosphorylation by Akt
triggers SCF complex formation and E3 ligase activity
Liu et al., Biochem Biophys Res Commun 2009
(Ovarian Neoplasms) :
MEK1 independent activation of MAPK and
MEK1 dependent activation of p70 S6 kinase by
stem cell factor (SCF) in ovarian cancer cells
Shirani et al., Cardiology journal 2009
(Aortic Diseases...) :
Studies have suggested that
flow mediated dilation (FMD) is
impaired in
SCF
Aerbajinai et al., Blood 2009
(MAP Kinase Signaling System) :
We found that
COUP-TFII expression is
suppressed by
SCF through phosphorylation of serine/threonine phosphatase ( PP2A ) and correlated well with fetal hemoglobin induction ... Our data show that
SCF stimulates Erk1/2 MAPK signaling pathway, which regulates the downstream repressor COUP-TFII by inhibiting serine/threonine phosphatase 2A activity, and that decreased
COUP-TFII expression
resulted in gamma-globin reactivation in adult erythropoiesis
Bordeaux-Rego et al., Stem Cells Dev 2010
:
The presence of
SCF , Flt3-L, and TPO are
essential for
CD133+ and/or CD34+ cells ex vivo expansion ; however, IL-3 and IL-6 influence has not yet been clearly established
Li et al., Braz J Med Biol Res 2009
(Diabetes Mellitus, Experimental) :
SCF expression was lower in the diabetic cortex than in the normal cortex ; however,
insulin increased the expression of SCF in the diabetic cortex
Suyama et al., Oncol Rep 2010
(Lung Neoplasms...) :
Using c-kit positive SCLC cells ( H209 and H69 cells ) and SCF as a model of the autocrine mechanisms, the
effects of
SCF , LY294002, PD98059 or STI571 on Akt and
Erk were assessed by Western blot analysis ... Using c-kit positive SCLC cells ( H209 and H69 cells ) and SCF as a model of the autocrine mechanisms, the
effects of
SCF , LY294002, PD98059 or STI571 on
Akt and Erk were assessed by Western blot analysis
Sánchez et al., J Med Food 2010
(Disease Models, Animal...) :
In addition,
SCF reduced plasma glucose and
insulin , and as a consequence the insulin resistance was also decreased
Zeuner et al., Cell Death Differ 2011
:
In cultures of primary human differentiating erythroblasts, we found that
SCF induces an increase in the expression of
Notch2 , a member of the Notch family implicated in the control of cell growth and differentiation ...
SCF also
induced the expression of Hes-1 and
GATA-2 , which may contribute to transduce Notch2 signals in response to SCF ...
SCF also
induced the expression of
Hes-1 and GATA-2, which may contribute to transduce Notch2 signals in response to SCF
Feuser et al., Mol Immunol 2011
:
The combined challenge of mast cells with IL-4,
SCF and Fc?RI cross linking substantially
up-regulated activation of
Akt , whereas blocking of Akt inhibited the pronounced production and release of IL-8 in response to the three mast cell agonists
Enomoto et al., Am J Pathol 2011
:
In addition, we showed that
MIF stimulated
stem cell factor (SCF) release in keratinocytes ; however, MIF had no effect on the release of endothelin-1 or prostaglandin E2 in keratinocytes
Zhang et al., Cancer Lett 2011
(Anoxia...) :
Furthermore, the expression of
HIF-1a induced by
SCF is not dependent on the oxygen level, but rather on both the PI3K/Akt and Ras/MEK/ERK signaling pathways ... In conclusion, under normoxic conditions,
SCF/c-kit binding
increases expression of
HIF-1a through the PI3K/Akt and Ras/MEK/ERK pathways, and the accumulation of HIF-1a up-regulates expression of invasion related genes that augment the invasiveness of pancreatic cancer, a fatal cancer
Chen et al., Mol Cell Biol 2011
(Pseudomonas Infections) :
Here we show that
calmodulin inhibits a new
SCF ( Skp1-Cullin-F-box ) E3 ligase component, FBXL2
Jeong et al., PloS one 2011
(Hypersensitivity...) :
KMP6 and hesperidin inhibited
SCF induced p38
MAPK activation
Dong et al., Pharmazie 2011
:
Regulation of AMH and
SCF expression in human granulosa cells by
GnRH agonist and antagonist
Mita et al., Mol Cell Biol 2011
(Prostatic Neoplasms) :
Repression of AR-mediated transcription is achieved, in part, by
URI binding and
regulation of
androgen receptor trapped clone 27 ( Art-27 ), a previously characterized AR corepressor
Vaites et al., Mol Cell Biol 2011
(Cell Transformation, Neoplastic...) :
Collectively, these data reveal a requisite
role for the
SCF ( Fbx4 ) E3 ubiquitin ligase in regulating
cyclin D1 accumulation, consistent with tumor suppressive function in vivo
Rådinger et al., J Immunol 2011
:
The decrease in stem cell factor (SCF) mediated survival in the GSK3ß knockdown HuMCs was reflected by enhancement of SCF withdrawal induced apoptosis, as determined by Annexin V staining and caspase cleavage, and this was associated with a pronounced reduction in
SCF mediated phosphorylation of Src homology 2 domain containing phosphatase 2 and ERK1/2 and reduced expression of the antiapoptotic proteins
Bcl-xl and Bcl-2 ... The decrease in stem cell factor (SCF) mediated survival in the GSK3ß knockdown HuMCs was reflected by enhancement of SCF withdrawal induced apoptosis, as determined by Annexin V staining and caspase cleavage, and this was associated with a pronounced reduction in
SCF mediated phosphorylation of Src homology 2 domain containing phosphatase 2 and
ERK1/2 and reduced expression of the antiapoptotic proteins Bcl-xl and Bcl-2 ... The decrease in stem cell factor (SCF) mediated survival in the GSK3ß knockdown HuMCs was reflected by enhancement of SCF withdrawal induced apoptosis, as determined by Annexin V staining and caspase cleavage, and this was associated with a pronounced reduction in
SCF mediated phosphorylation of Src homology 2 domain containing phosphatase 2 and ERK1/2 and reduced expression of the antiapoptotic proteins Bcl-xl and
Bcl-2
Nakajima et al., J Nat Med 2012
:
Signaling analysis with immunoblots revealed that in human melanocytes or human melanoma cells treated with WSE, there was a marked deficiency in
SCF stimulated phosphorylation of ERK, MITF and
CREB , but not of Raf-1 and MEK ... Signaling analysis with immunoblots revealed that in human melanocytes or human melanoma cells treated with WSE, there was a marked deficiency in
SCF stimulated phosphorylation of
ERK , MITF and CREB, but not of Raf-1 and MEK ... Signaling analysis with immunoblots revealed that in human melanocytes or human melanoma cells treated with WSE, there was a marked deficiency in
SCF stimulated phosphorylation of ERK,
MITF and CREB, but not of Raf-1 and MEK ... Signaling analysis with immunoblots revealed that in human melanocytes or human melanoma cells treated with WSE, there was a marked deficiency in
SCF stimulated phosphorylation of ERK, MITF and CREB, but not of
Raf-1 and MEK
Piao et al., Differentiation 2012
:
We also observe that
SCF and G-CSF
promote neuronal differentiation and inhibit astroglial differentiation at the early stage of differentiation
Hoellein et al., Mol Cell Biol 2012
:
Cks1 is an
activator of the
SCF ( Skp2 ) ubiquitin ligase complex that targets the cell cycle inhibitor p27 ( Kip1 ) for degradation
Oliveira et al., J Endod 2012
(MAP Kinase Signaling System) :
LPS induced
SCF mRNA expression and production in OD-like cells occur via leukotriene production or cytokine and/or chemokine formation, activating the p42/44, p38, and PI3K pathways
Broxmeyer et al., Blood 2012
:
In support of this,
CD1d was
required for the
SCF and Flt3 ligand synergistic enhancement of CSF induction of HPC colony formation and for HPC response to myelosuppressive chemokines ... In support of this,
CD1d was
required for the
SCF and Flt3 ligand synergistic enhancement of CSF induction of HPC colony formation and for HPC response to myelosuppressive chemokines
Nakajima et al., Arch Dermatol Res 2012
(Hyperpigmentation) :
Real-time RT-PCR and western blotting of Ax-treated HEEs revealed that the
SCF stimulated expression of tyrosinase (TYR), TYR related protein-1 (TYRP1), and
Pmel17 , as well as microphthalmia associated transcription factor ( MITF ), is significantly suppressed by Ax at the transcriptional and translational levels ... Real-time RT-PCR and western blotting of Ax-treated HEEs revealed that the
SCF stimulated expression of tyrosinase (TYR), TYR related protein-1 (TYRP1), and Pmel17, as well as microphthalmia associated
transcription factor ( MITF ), is significantly suppressed by Ax at the transcriptional and translational levels ... Real-time RT-PCR and western blotting of Ax-treated HEEs revealed that the
SCF stimulated expression of
tyrosinase (TYR) , TYR related protein-1 (TYRP1), and Pmel17, as well as microphthalmia associated transcription factor ( MITF ), is significantly suppressed by Ax at the transcriptional and translational levels ... Real-time RT-PCR and western blotting of Ax-treated HEEs revealed that the
SCF stimulated expression of tyrosinase (TYR),
TYR related protein-1 (TYRP1) , and Pmel17, as well as microphthalmia associated transcription factor ( MITF ), is significantly suppressed by Ax at the transcriptional and translational levels ... Real-time RT-PCR and western blotting of Ax-treated HEEs revealed that the
SCF stimulated expression of tyrosinase (TYR), TYR related protein-1 (TYRP1), and Pmel17, as well as microphthalmia associated transcription factor (
MITF ), is significantly suppressed by Ax at the transcriptional and translational levels
Jelly et al., Cancer cell international 2012
:
Bcl-2 expression was significantly increased in the
presence of adriamycin and
SCF ( p? < ?0.038 ) and decreased in the presence of adriamycin and
anti-SCF ...
Bcl-2 expression was significantly increased in the
presence of adriamycin and
SCF ( p? < ?0.038 ) and decreased in the presence of adriamycin and anti-SCF ... However, in the
presence of both adriamycin and
SCF the expression of
annexin V was decreased, but was significantly increased in the presence of adriamycin and anti-SCF ( p? < ?0.001 ), adriamycin, anti-SCF and SCF combined and adriamycin alone ... However, in the
presence of both adriamycin and
SCF the expression of annexin V was decreased, but was significantly increased in the presence of adriamycin and anti-SCF ( p? < ?0.001 ), adriamycin,
anti-SCF and SCF combined and adriamycin alone ... Our results demonstrate that
anti-SCF with low dose of adriamycin
reduces Bcl-2 expression in MCF-7/AdrRes cells and increases annexin V expression in both MCF7/AdrRes and MCF-7 cells ... Our results demonstrate that
anti-SCF with low dose of adriamycin reduces Bcl-2 expression in MCF-7/AdrRes cells and
increases annexin V expression in both MCF7/AdrRes and MCF-7 cells
Bai et al., World J Gastroenterol 2012
(Gastrointestinal Neoplasms...) :
To clarify the biological role of
stem cell factor (SCF) mediated wild-type
KIT receptor activation in gastrointestinal stromal tumor ( GIST ) growth
Wang et al., Neoplasia (New York, N.Y.) 2012
(Cell Transformation, Neoplastic...) :
Furthermore, three independent laboratories including our own have demonstrated that the stability of
DEPTOR is
controlled by the
SCF ( ß-TrCP ) E3 ubiquitin ligase and deregulated DEPTOR destruction might contribute to hyperactivation of mTOR in pathologic conditions including cancer
Enchev et al., Cell reports 2012
:
Taken together, the results provide a model for how reciprocal regulation sensitizes
CSN to the SCF assembly state and
inhibits a catalytically competent
SCF until a ubiquitination substrate drives its own degradation by displacing CSN, thereby promoting cullin neddylation and substrate ubiquitination
Bhaskaran et al., Mol Cell Biol 2013
:
In vitro reconstitution showed that the
role of
SCF ( Fbw7a ) in
cyclin E degradation, rather than ubiquitylation, is to serve as a cofactor of the prolyl cis-trans isomerase Pin1 in the isomerization of a noncanonical proline-proline bond in the cyclin E phosphodegron
Tanaka et al., Curr Pharm Des 2013
:
The ubiquitin ligase activity of the
SCF complex requires
NEDD8 to covalently bind to cullins
Wakabayashi et al., J Dermatol Sci 2013
(Lentigo) :
Real-time RT-PCR and Western blotting of these NCM treated HEEs unexpectedly revealed that the
EDN1+SCF stimulated steady-state levels of tyrosinase (TYR), TYR related protein-1, dopachrome tautomerase and
PMEL17 as well as microphthalmia associated transcription factor ( MITF ) were significantly attenuated at the transcriptional and translational levels without any cytotoxic effects on keratinocytes and melanocytes in the HEEs ... Real-time RT-PCR and Western blotting of these NCM treated HEEs unexpectedly revealed that the
EDN1+SCF stimulated steady-state levels of tyrosinase (TYR), TYR related protein-1, dopachrome tautomerase and PMEL17 as well as microphthalmia associated
transcription factor ( MITF ) were significantly attenuated at the transcriptional and translational levels without any cytotoxic effects on keratinocytes and melanocytes in the HEEs ... Real-time RT-PCR and Western blotting of these NCM treated HEEs unexpectedly revealed that the
EDN1+SCF stimulated steady-state levels of
tyrosinase (TYR) , TYR related protein-1, dopachrome tautomerase and PMEL17 as well as microphthalmia associated transcription factor ( MITF ) were significantly attenuated at the transcriptional and translational levels without any cytotoxic effects on keratinocytes and melanocytes in the HEEs ... Real-time RT-PCR and Western blotting of these NCM treated HEEs unexpectedly revealed that the
EDN1+SCF stimulated steady-state levels of tyrosinase (TYR), TYR related protein-1,
dopachrome tautomerase and PMEL17 as well as microphthalmia associated transcription factor ( MITF ) were significantly attenuated at the transcriptional and translational levels without any cytotoxic effects on keratinocytes and melanocytes in the HEEs ... Pre-treatment of cultured normal human melanocytes with the NCMs interrupted the
EDN1+SCF induced stimulation of steady-state levels of MITF at the transcriptional and translational levels and
TYR activity without any direct inhibitory effect on the catalytic activity of TYR in vitro ... Pre-treatment of cultured normal human melanocytes with the NCMs interrupted the
EDN1+SCF induced stimulation of steady-state levels of
MITF at the transcriptional and translational levels and TYR activity without any direct inhibitory effect on the catalytic activity of TYR in vitro
Lewis et al., Clin Exp Allergy 2013
:
Mediator release
induced by
SCF was accompanied by the up-regulation of the activation marker, CD63
Shin et al., Exp Mol Med 2013
(Melanoma, Experimental) :
Glyceollins inhibited melanin synthesis, as well as the expression and activity of
tyrosinase induced by
SCF , in a dose dependent manner in B16F10 melanoma cells
Lu et al., Food Chem Toxicol 2013
:
HEE suppressed
SCF/IL-10/LPS induced
PGD2 generation, and concomitantly reduced COX-2 protein expression dose-dependently
Patel et al., Biometals 2013
:
In addition, both
ATP7A deficiency and neocuproine treatment
had no effect on GM-CSF, IL-10, IL-11, IL-7, OSM, and
SCF
Wiseman et al., J Biol Chem 2013
(Second Messenger Systems) :
Proteasomal Degradation of
Eukaryotic Elongation Factor-2 Kinase ( EF2K ) Is
Regulated by cAMP-PKA Signaling and the SCFßTRCP
Ubiquitin E3 Ligase ... Proteasomal Degradation of Eukaryotic
Elongation Factor-2 Kinase ( EF2K ) Is
Regulated by cAMP-PKA Signaling and the SCFßTRCP
Ubiquitin E3 Ligase
Hurst et al., J Biol Chem 2013
:
Here, we show that the Skp1/Cullin/F-box ubiquitin ligase
SCF ( Cdc4 ) and the ubiquitin protease Ubp3
regulate Ste7 ubiquitination and signal specificity
Huang et al., PloS one 2013
:
Unexpectedly however, csn3-3 plants are not defective in either the
CSN mediated deneddylation of CUL1 or in
SCF ( TIR1 ) -mediated degradation of Aux/IAA proteins
Cepeda et al., EMBO Mol Med 2013
(Breast Neoplasms) :
SCF ( FBXO28 ) activity and stability are
regulated during the cell cycle by CDK1/2 mediated phosphorylation of
FBXO28 , which is required for its efficient ubiquitylation of MYC and downsteam enhancement of the MYC pathway
Fukushima et al., Cell reports 2013
:
Here, we continue to report that
SCF ( ß-TRCP ) reciprocally
controls APC/Cdh1 activity by governing Cdh1 ubiquitination and subsequent degradation ... Here, we continue to report that
SCF ( ß-TRCP ) reciprocally
controls APC/Cdh1 activity by governing Cdh1 ubiquitination and subsequent degradation
Hirata et al., Development 1993
:
We investigated the in vitro
effect of
stem cell factor (SCF) , the ligand for c-kit receptor, on DRGs. Recombinant murine
SCF ( rmSCF ) induced the outgrowth of c-kit positive neurites from DRGs of normal ( +/+ ) embryos
Whetton et al., J Cell Biol 1994
(Second Messenger Systems) :
Furthermore, M-CSF, but not
SCF , can
increase the levels of
PKC alpha ( PKC alpha ) expression and stimulate the translocation of PKC alpha to the nucleus
Neta et al., J Immunol 1994
:
SCF , unlike IL-1 does not
induce hemopoietic CSFs and
IL-6 or gene expression of a scavenging mitochondrial enzyme manganese superoxide dismutase in the bone marrow, suggesting that SCF and IL-1 radioprotect by distinct pathways
Blume-Jensen et al., J Biol Chem 1994
:
In contrast, the
SCF stimulated
Kit/SCF-R associated efficiently with, and induced tyrosine phosphorylation of, the p85 alpha regulatory subunit of phosphatidyl inositide-3'-kinase ( PI-3'-kinase )
Tang et al., Mol Cell Biol 1994
:
Conversely, the cytoplasmic kinase
JAK2 is
activated by IL-3 but not by
SCF stimulation
Jhun et al., J Biol Chem 1995
:
MATK-SH2, but not MATK-SH3, precipitated the tyrosine phosphorylated c-Kit ( molecular mass of 145 kDa ) in
KL/SCF stimulated
CMK cells
Shiohara et al., Blood 1993
:
Primitive progenitors responding to SCF + IFN-gamma were suggested to be supported by
SCF in the early stage of development and
require IFN-gamma for subsequent growth
Koike et al., J Immunol 1993
(Second Messenger Systems) :
Taken together,
SCF induces the activation of
PLD through the protein tyrosine kinase pathway without activation of phosphoinositide-specific phospholipase C
Sperr et al., Int Arch Allergy Immunol 1993
:
A monoclonal antibody to SCF was found to inhibit the
effects of
SCF on
MCs
Erickson-Miller et al., Stem Cells 1993
:
We describe studies in which
SCF stimulated murine
CFU-Meg alone and in the presence of IL-3
Pallard et al., EMBO J 1995
(Leukemia, Megakaryoblastic, Acute) :
In contrast, neither interferon (IFN)-gamma nor the mitogenic
stem cell factor (SCF) activated
STAT5 , although IFN-gamma did activate STAT1 in those cells
Reddy et al., Blood 1996
(Leukemia, Myeloid) :
At low concentrations
SCF caused a synergistic increase in IL-3 dependent induction of both CaM-BP68 and
thymidine kinase activity ... At low concentrations
SCF caused a synergistic increase in IL-3 dependent induction of both
CaM-BP68 and thymidine kinase activity
O'Farrell et al., Blood 1996
:
In B6M cells, both IL-3 and
SCF stimulate phosphorylation of
Shc and activation of the Ras, Raf-1, MAPK pathway ... Interestingly, IL-3 plus
SCF synergistically
activate MAPK
Weiler et al., Blood 1996
:
These data demonstrate that
SCF induces tyrosine phosphorylation of
JAK2 and suggest that JAK2 is a component of the SCF signal transduction pathway
Langtimm-Sedlak et al., Dev Biol 1996
:
Our data suggest that
SCF affects neural crest cell development at multiple levels and that survival of melanogenic cells is
mediated by a combination of SCF and a neurotropin, rather than by
SCF alone
Pyatt et al., Mol Pharmacol 1996
:
Support for this conclusion came from experiments demonstrating that 1 ) mutant mice strains constitutively lacking a SCF-responsive population of HPC [ White spotted ( W/WV ) and Steel ( SI/SId ) ] are unresponsive to xanthine/XO- and
SCF induced enhancement of
GM-CSF and 2 ) 3,4-epoxybutene, which selectively abrogates SCF synergy with GM-CSF, inhibits xanthine/XO induced enhancement
Nakahata et al., Cancer Chemother Pharmacol 1996
:
The sIL-6R-IL-6 complex, but not sIL-6R or
IL-6 alone, in the
presence of
stem-cell factor (SCF) produced dramatic increases in the populations of various cell lineages, including erythroid cells and various hematopoietic progenitors, in suspension culture
Thomas et al., Clin Exp Allergy 1996
:
Changes in the level of mastocytoma mRNA for
TNF alpha in
response to stimulation with
SCF or fibroblast conditioned media for up to 12 weeks were studied using Northern analysis and changes in the level of TNF alpha protein expression on immunoblot and immunocytochemistry
Imamura et al., Exp Hematol 1996
:
Furthermore,
LIF+SCF+IL-1 beta induced increased IL-3 and GM-CSF mRNA expression in hematopoietic cells but
induced decreased macrophage inflammatory protein 1 alpha (
MIP1 alpha ) mRNA expression as compared with SCF+IL-1 beta +IL-3 ... Furthermore,
LIF+SCF+IL-1 beta
induced increased
IL-3 and GM-CSF mRNA expression in hematopoietic cells but induced decreased macrophage inflammatory protein 1 alpha ( MIP1 alpha ) mRNA expression as compared with SCF+IL-1 beta +IL-3
Huss et al., Exp Mol Pathol 1995
:
While differentiating, these cells start to express CD34 and DR. Differentiation is
induced by
stem cell factor (SCF) , while
interleukin-6 (IL-6) inhibits differentiation, but promotes proliferation ...
SCF induces a transient expression of p27kip-1, while no
p27kip-1 is detectable in the presence of IL-6 or an anti-SCF monoclonal antibody ...
SCF induces a transient expression of p27kip-1, while no
p27kip-1 is detectable in the presence of IL-6 or an anti-SCF monoclonal antibody
Ramsfjell et al., Blood 1996
:
SCF potently
enhanced Tpo stimulated production of high-ploidy
Mks from Lin- Sca-1+ progenitors, whereas the increased growth response obtained when combining Tpo with FL did not translate into increased Mk production
Dolzhanskiy et al., Blood 1997
:
SCF alone had no effect on MK proliferation in the absence of TPO, but
increased both MK number and CFU-MK by 1.5- to 2.0-fold in the presence of
TPO
Jacobs-Helber et al., J Biol Chem 1997
(Leukemia, Erythroblastic, Acute) :
A recent report ( Wu, H., Klingmuller, U., Besmer, P., and Lodish, H. F. ( 1995 ) Nature 377, 242-246 ) documents the interaction of the erythropoietin (EPO) receptor ( EPOR ) with the stem cell factor (SCF) receptor ( c-KIT ) and suggests that
SCF acts through the
EPOR ... However,
SCF dependent phosphorylation of the
EPOR did not initiate an EPO-like intracellular signal ...
SCF induced proliferation,
SHC phosphorylation, and activation of ERK-1 but did not activate the JAK/STAT pathway ...
SCF induced proliferation, SHC phosphorylation, and activation of
ERK-1 but did not activate the JAK/STAT pathway ... SCF stimulated SHC phosphorylation and ERK-1 activation independent of the EPOR in cells where the EPOR was down-regulated ; the presence of the EPOR appeared to facilitate
SCF activation of
SHC and ERK-1 ... SCF stimulated SHC phosphorylation and ERK-1 activation independent of the EPOR in cells where the EPOR was down-regulated ; the presence of the EPOR appeared to facilitate
SCF activation of SHC and
ERK-1
Ryan et al., Exp Hematol 1997
:
These data indicate that
SCF stimulation of Kit
leads to activation of
STAT-5 DNA binding with kinetics distinct from Epo mediated stimulation
Cardier et al., Stem Cells 1997
:
Moreover,
GM-CSF in the
presence of
SCF and IL-3 potentiates the HPP-CFC formation ( i.e., an increase of 3.1-fold compared to the effect induced by SCF+IL-3 ) with strong inhibitory effects on the number and size of megakaryocyte colonies
Grgurevich et al., Growth Factors 1997
:
Previous reports demonstrating expression of Hyl and Matk in hematopoietic lineages led us to investigate the regulation of
Lsk expression in
response to
stem cell factor (SCF) and granulocyte-macrophage colony stimulating factor ( GM-CSF ) in M07e, a human leukemic cell line ... These data demonstrate differential regulation of Csk family members by cytokines and suggest a role for Lsk/
Hyl/Matk in responses
mediated by
SCF and PMA
Kozawa et al., Eur J Biochem 1997
(Second Messenger Systems) :
In contrast,
phospholipase D ( PLD ) is activated in
response to
SCF in a dose dependent manner
Lorgeot et al., Cytokine 1997
:
Interleukin 1alpha (IL-1alpha), IL-1beta, IL-3, IL-6, IL-8, tumour necrosis factor ( TNF-alpha ) and
SCF ( both at 10 ng/ml )
stimulate LIF production
Joneja et al., Blood 1997
:
In addition and in contrast to signaling in Mo7e and BM6 cell lines, in FDC2-ER cells
SCF and Epo each were shown to rapidly
activate Pim 1 gene expression ... These studies of SCF and Epo signaling in FDC2-wt-ER cells serve to functionally map the ERB1 region as a c-kit-interactive domain, suggest that
Pim1 might
contribute to
SCF and Epo mitogenic synergy and support the notion that SCF and Epo may act in opposing ways during red cell differentiation
Deberry et al., Biochem J 1997
:
We recently found that the Janus kinase
Jak2 associates with c-kit and that
SCF induces rapid and transient phosphorylation of Jak2 ... Here we present evidence that
SCF activates the transcription factor
Stat1 ... These results demonstrate that
Stat1 is activated in
response to
SCF , and suggest that Stat1 is a component of the SCF signal-transduction pathway
Valent et al., Int Arch Allergy Immunol 1998
(Edema...) :
The c-kit ligand
SCF upregulates uPAR expression, and the release of
tPA from MC ... The c-kit ligand
SCF upregulates
uPAR expression, and the release of tPA from MC
Qu et al., Int Arch Allergy Immunol 1998
:
Expression of
FGF-2 may be a ubiquitous feature of MC in other species in addition to humans, and can be selectively
regulated by
SCF , TGF-beta and TNF-alpha
Imokawa et al., Biochem J 1998
:
HGF and
SCF , but not bFGF, were markedly increased in culture medium in the
presence of
IL-1alpha , and this stimulatory effect was confined to young human fibroblasts
Harigae et al., Genes Cells 1998
:
In contrast,
GATA-1 protein was expressed in PMCs and BMMCs after culture in the
presence of IL3 and
SCF ... In contrast,
GATA-1 protein was expressed in PMCs and BMMCs after culture in the
presence of IL3 and
SCF
Sato et al., J Biol Chem 1998
(Leukemia, Megakaryoblastic, Acute) :
SCF activated the human EPO receptor promoter and induced
EPO receptor gene expression ...
SCF activated the human
EPO receptor promoter and induced EPO receptor gene expression ... Given these results, we speculate that HML/SE cells acquired responsiveness to EPO via the
EPO receptor
induced by
SCF
Muraguchi et al., Leukemia & lymphoma 1998
:
Among the cytokines, IL-3, IL-6, and IL-7, but not IL-2, IL-4,
SCF , GM-CSF
induces RAG activation
Tisdale et al., Blood 1998
:
Stem cell factor (SCF)/granulocyte colony stimulating factor ( G-CSF ) -mobilized and CD34 enriched PB cells were divided into two equal aliquots and transduced with one of two retroviral vectors carrying the neomycin-resistance gene ( neo ) for 4 days in the
presence of
interleukin-3 (IL-3) , IL-6, and SCF in the first 5 animals, IL-3/IL-6/SCF/Flt-3 ligand (FLT) in 2 subsequent animals, or
IL-3/IL-6/SCF/FLT plus an autologous stromal monolayer ( STR ) in the final 2 ... Stem cell factor (SCF)/granulocyte colony stimulating factor ( G-CSF ) -mobilized and CD34 enriched PB cells were divided into two equal aliquots and transduced with one of two retroviral vectors carrying the neomycin-resistance gene ( neo ) for 4 days in the
presence of interleukin-3 (IL-3),
IL-6 , and SCF in the first 5 animals, IL-3/IL-6/SCF/Flt-3 ligand (FLT) in 2 subsequent animals, or
IL-3/IL-6/SCF/FLT plus an autologous stromal monolayer ( STR ) in the final 2 ... Stem cell factor (SCF)/granulocyte colony stimulating factor ( G-CSF ) -mobilized and CD34 enriched PB cells were divided into two equal aliquots and transduced with one of two retroviral vectors carrying the neomycin-resistance gene ( neo ) for 4 days in the
presence of interleukin-3 (IL-3), IL-6, and
SCF in the first 5 animals, IL-3/IL-6/SCF/Flt-3 ligand (FLT) in 2 subsequent animals, or
IL-3/IL-6/SCF/FLT plus an autologous stromal monolayer ( STR ) in the final 2
Sui et al., Blood 1998
:
Together, the data suggest that synergistic
activation of
MAPK by
SCF and EPO is essential for expanded erythropoiesis
Ishizuka et al., J Immunol 1998
:
Coligation of SCFR augmented Fc epsilonRI mediated activation of MAP kinases, especially JNK activation, and SCF augmented Fc epsilonRI mediated TNF-alpha production in MC/9 cells, although
SCF alone did not
induce TNF-alpha production
Krystal et al., Cancer Res 1998
(Carcinoma, Small Cell...) :
Stimulation of Kit with recombinant
SCF resulted in a rapid 3-6-fold increase in the specific activity of
Lck , which was similar in magnitude to the activation of Lck resulting from the cross linking of the T-cell receptor complex of Jurkat cells
Umekage et al., J Reprod Immunol 1998
:
Results suggest that
SCF produced in the decidua
increases the expression of the IL-2Ralpha which is usually present in smaller amounts than other two IL-2R chains on decidual CD16- CD56bright NK cells, and thereby promotes the proliferation of these cells in response to low concentrations of IL-2, resulting in an increase of the high affinity
IL-2Rs
Spyridonidis et al., Blood 1999
(Ascites...) :
Expansion of clonogenic tumor cells was seen in the
presence of
SCF + IL-1beta + IL-3 + IL-6 + EPO, but was suppressed by
TGF-beta1
Zhang et al., Blood 1999
:
In the absence of TNFalpha, TGF-beta1 markedly suppressed the expression of CIITA and CCR7 mRNAs in GM-CSF +
SCF stimulated
Lin-c-kit+ HPCs at either day 6 or day 12 and induced the differentiation solely into monocytes/macrophages as evident in morphology, active phagocytic, and endocytic activities