Nesheim et al., J Biol Chem 1981
:
In the presence of phospholipid and Ca2+ , factor Va and factor Xa interacted with 1 : 1 stoichiometry, an interaction characterized by markedly enhanced polarization
Govers-Riemslag et al., Eur J Biochem 1994
:
Stimulation of prothrombin activation by phosphatidylcholine vesicles was particularly observed ( a ) with phosphatidylcholine molecules that contained unsaturated hydrocarbon side chains, ( b ) in the presence of factor Va , ( c ) at low ionic strength and ( d ) when Ca2+ were present in the reaction medium
Long et al., J Biol Chem 1998
:
The cleavage requires the presence of phospholipid and Ca2+ , and is markedly inhibited by the presence of factor Va