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CFTR — PRKAB2

Text-mined interactions from Literome

Hallows et al., J Clin Invest 2000 : Because AMPK is a metabolic sensor in cells and responds to changes in cellular ATP, regulation of CFTR by AMPK may be important in inhibiting CFTR under conditions of metabolic stress, thereby linking transepithelial transport to cell metabolic state
Hallows et al., J Biol Chem 2003 (Ion Channel Gating) : Plasma membrane expression of CFTR , assessed by surface biotinylation, was not affected by AMPK activation
King et al., Am J Physiol Cell Physiol 2009 : To further characterize the mechanism of AMPK dependent regulation of CFTR , whole cell patch-clamp measurements were performed with PKA activation in Calu-3 cells expressing either constitutively active or dominant negative AMPK mutants ( AMPK-CA or AMPK-DN ) ... Baseline CFTR conductance in cells expressing AMPK-DN was substantially greater than controls, suggesting that tonic AMPK activity in these cells inhibits CFTR under basal conditions ... Phosphorylation studies of CFTR in human embryonic kidney-293 cells using tetracycline-inducible expression of AMPK-DN demonstrated AMPK dependent phosphorylation of CFTR in vivo ... Thus, AMPK dependent CFTR phosphorylation renders the channel resistant to activation by PKA and PKC without preventing phosphorylation by these kinases ... Ser-to-Ala mutation at this site enhanced baseline CFTR activity and rendered CFTR resistant to inhibition by AMPK, suggesting that AMPK phosphorylation at Ser768 is required for its inhibition of CFTR ... In summary, our findings indicate that AMPK dependent phosphorylation of CFTR inhibits CFTR activation by PKA, thereby tuning the PKA-responsiveness of CFTR to metabolic and other stresses in the cell
Murphy et al., Am J Physiol Cell Physiol 2009 : Finally, inhibition of the cystic fibrosis transmembrane regulator ( CFTR ) Cl ( - ) conductance blocked depolarization of GI neurons to decreased glucose or AMPK activation, whereas decreased glucose, AMPK activation, and 8-Br-cGMP increased VMH CFTR phosphorylation
King et al., J Biol Chem 2012 : Taken together, these results suggest that NDPK-A exists in a functional cellular complex with AMPK and CFTR in airway epithelia, and NDPK-A catalytic function is required for the AMPK dependent regulation of CFTR