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FEN1 — PCNA

Pathways - manually collected, often from reviews:

• Reactome Reaction: FEN1 → PCNA (reaction) Harrington et al., J Biol Chem 1995*, Harrington et al., EMBO J 1994*
• Reactome Reaction: FEN1 → PCNA (direct_complex)

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

• IRef Bind Interaction: PCNA — FEN1 Sakurai et al., EMBO J 2005*
• IRef Bind Interaction: PCNA — FEN1 Ohta et al., J Biol Chem 2002, Gary et al., J Biol Chem 1997*
• IRef Bind Interaction: PCNA — FEN1 Warbrick et al., Nucleic Acids Res 1998*
• IRef Bind Interaction: PCNA — FEN1 Warbrick et al., Oncogene 1997*
• IRef Bind_translation Interaction: PCNA — FEN1 (far western blotting) Warbrick et al., Oncogene 1997*
• IRef Bind_translation Interaction: PCNA — FEN1 (x-ray crystallography) Sakurai et al., EMBO J 2005*
• IRef Bind_translation Interaction: PCNA — FEN1 (affinity chromatography technology) Ohta et al., J Biol Chem 2002, Gary et al., J Biol Chem 1997*
• IRef Bind_translation Interaction: PCNA — FEN1 (affinity chromatography technology) Ohta et al., J Biol Chem 2002
• IRef Bind_translation Interaction: PCNA — FEN1 (two hybrid) Warbrick et al., Nucleic Acids Res 1998*
• IRef Bind_translation Interaction: PCNA — FEN1 (unspecified method) Warbrick et al., Oncogene 1997*
• IRef Bind_translation Interaction: PCNA — FEN1 (affinity chromatography technology) Warbrick et al., Oncogene 1997*
• IRef Bind_translation Interaction: PCNA — FEN1 (coimmunoprecipitation) Warbrick et al., Oncogene 1997*
• IRef Bind_translation Interaction: PCNA — FEN1 (two hybrid) Warbrick et al., Oncogene 1997*
• IRef Biogrid Interaction: PCNA — FEN1 (association, x-ray crystallography) Zhang et al., Cell cycle (Georgetown, Tex.) 2012*
• IRef Biogrid Interaction: PCNA — FEN1 (physical association, affinity chromatography technology) Pichler et al., PloS one 2012*
• IRef Biogrid Interaction: PCNA — FEN1 (physical association, affinity chromatography technology) Takawa et al., Cancer Res 2012*
• IRef Biogrid Interaction: PCNA — FEN1 (direct interaction, two hybrid) Wu et al., Nucleic Acids Res 1996*
• IRef Biogrid Interaction: PCNA — FEN1 (direct interaction, pull down) Wu et al., Nucleic Acids Res 1996*
• IRef Biogrid Interaction: PCNA — FEN1 (association, x-ray crystallography) Sakurai et al., EMBO J 2005*
• IRef Biogrid Interaction: PCNA — FEN1 (direct interaction, fluorescent resonance energy transfer) Sharma et al., Mol Biol Cell 2004*
• IRef Biogrid Interaction: PCNA — FEN1 (direct interaction, pull down) Henneke et al., Oncogene 2003*
• IRef Biogrid Interaction: PCNA — FEN1 (direct interaction, pull down) Hasan et al., Mol Cell 2001*
• IRef Biogrid Interaction: PCNA — FEN1 (direct interaction, pull down) Jónsson et al., EMBO J 1998*
• IRef Biogrid Interaction: PCNA — FEN1 (direct interaction, pull down) Gary et al., J Biol Chem 1997*
• IRef Biogrid Interaction: PCNA — FEN1 (direct interaction, two hybrid) Chen et al., Proc Natl Acad Sci U S A 1996*
• IRef Biogrid Interaction: PCNA — FEN1 (direct interaction, pull down) Chen et al., Proc Natl Acad Sci U S A 1996*
• IRef Biogrid Interaction: PCNA — FEN1 (association, biochemical) Chen et al., Proc Natl Acad Sci U S A 1996*
• IRef Biogrid Interaction: PCNA — FEN1 (physical association, affinity chromatography technology) Chen et al., Proc Natl Acad Sci U S A 1996*
• IRef Biogrid Interaction: PCNA — FEN1 (direct interaction, pull down) Dianova et al., Biochemistry 2001*
• IRef Biogrid Interaction: PCNA — FEN1 (physical association, affinity chromatography technology) Dianova et al., Biochemistry 2001*
• IRef Biogrid Interaction: PCNA — FEN1 (direct interaction, two hybrid) Yu et al., Oncogene 2001*
• IRef Biogrid Interaction: PCNA — FEN1 (physical association, affinity chromatography technology) Friedrich-Heineken et al., J Mol Biol 2005*
• IRef Biogrid Interaction: PCNA — FEN1 (physical association, affinity chromatography technology) Wang et al., Nat Cell Biol 2006*
• IRef Biogrid Interaction: PCNA — FEN1 (direct interaction, far western blotting) Warbrick et al., Oncogene 1997*
• IRef Biogrid Interaction: PCNA — FEN1 (physical association, affinity chromatography technology) Warbrick et al., Oncogene 1997*
• IRef Biogrid Interaction: PCNA — FEN1 (association, x-ray crystallography) Bruning et al., Structure 2004*
• IRef Biogrid Interaction: PCNA — FEN1 (direct interaction, unspecified method) Bruning et al., Structure 2004*
• IRef Dip Interaction: PCNA — FEN1 (direct interaction, x-ray crystallography) Bruning et al., Structure 2004*
• IRef Dip Interaction: PCNA — FEN1 (direct interaction, biochemical) Gary et al., J Biol Chem 1997*
• IRef Dip Interaction: PCNA — FEN1 (direct interaction, isothermal titration calorimetry) Bruning et al., Structure 2004*
• IRef Dip Interaction: PCNA — FEN1 (direct interaction, two hybrid) Wu et al., Nucleic Acids Res 1996*
• IRef Dip Interaction: PCNA — FEN1 (direct interaction, affinity chromatography technology) Gary et al., J Biol Chem 1997*
• IRef Dip Interaction: PCNA — FEN1 (direct interaction, affinity chromatography technology) Wu et al., Nucleic Acids Res 1996*
• IRef Hprd Interaction: PCNA — FEN1 (two hybrid) Hasan et al., Mol Cell 2001*, Sharma et al., Mol Biol Cell 2004*, Kemeny et al., J Immunother (1991) 1992*, Li et al., J Biol Chem 1995*, Chen et al., Proc Natl Acad Sci U S A 1996*, Gary et al., J Biol Chem 1997*, Jónsson et al., EMBO J 1998*
• IRef Hprd Interaction: PCNA — FEN1 (in vivo) Hasan et al., Mol Cell 2001*, Sharma et al., Mol Biol Cell 2004*, Kemeny et al., J Immunother (1991) 1992*, Li et al., J Biol Chem 1995*, Chen et al., Proc Natl Acad Sci U S A 1996*, Gary et al., J Biol Chem 1997*, Jónsson et al., EMBO J 1998*
• IRef Hprd Interaction: PCNA — FEN1 (in vitro) Hasan et al., Mol Cell 2001*, Sharma et al., Mol Biol Cell 2004*, Kemeny et al., J Immunother (1991) 1992*, Li et al., J Biol Chem 1995*, Chen et al., Proc Natl Acad Sci U S A 1996*, Gary et al., J Biol Chem 1997*, Jónsson et al., EMBO J 1998*
• IRef Intact Interaction: Complex of 400 proteins (association, cross-linking study) Humphries et al., Science signaling 2009
• IRef Intact Interaction: PCNA — FEN1 (colocalization, fluorescent resonance energy transfer) Sharma et al., Mol Biol Cell 2004*
• IRef Intact Interaction: PCNA — FEN1 (physical association, fluorescent resonance energy transfer) Sharma et al., Mol Biol Cell 2004*
• IRef Intact Interaction: PCNA — FEN1 (physical association, two hybrid array) Boxem et al., Cell 2008*
• IRef Intact Interaction: PCNA — FEN1 (physical association, two hybrid pooling approach) Boxem et al., Cell 2008*
• IRef Ophid Interaction: PCNA — FEN1 (aggregation, confirmational text mining) Gary et al., J Biol Chem 1997*
• IRef Ophid Interaction: PCNA — FEN1 (aggregation, interologs mapping) Brown et al., Bioinformatics 2005

Text-mined interactions from Literome

Tom et al., J Biol Chem 2000 : PCNA stimulates FEN1 irrespective of the flap length ... These results indicate that PCNA does not direct FEN1 to the cleavage site from solution ... Overall, our results indicate that after FEN1 tracks to the cleavage site, PCNA enhances FEN1 binding stability, allowing for greater cleavage efficiency
Gomes et al., EMBO J 2000 : Like many other proliferating cell nuclear antigen ( PCNA ) -binding proteins, FEN1 interacts with the interdomain connector loop ( IDCL ) of PCNA, and PCNA greatly stimulates FEN1 activity ... In contrast, a C-terminal mutant pcna-90 ( PK252,253AA ) showed wild-type binding to FEN1 in solution, but poorly stimulated FEN1 activity ... In this DNA dependent binding assay, pcna-79 also stabilized retention of FEN1 , but pcna-90 was inactive
Shibata et al., J Biol Chem 2002 : Taken together, these findings suggest that FEN-1 plays an essential role in the DNA repair processes in mammalian cells and that this activity of FEN-1 is PCNA dependent
Henneke et al., Oncogene 2003 : Moreover, phosphorylation of Fen1 by Cdk1-Cyclin A abrogates its proliferating cell nuclear antigen ( PCNA ) binding thus preventing stimulation of Fen1 by PCNA
Chapados et al., Cell 2004 : Ordering of unstructured C-terminal regions in FEN-1 and PCNA creates an intermolecular beta sheet interface that directly links adjacent PCNA and DNA binding regions of FEN-1 and suggests how PCNA stimulates FEN-1 activity
Friedrich-Heineken et al., Nucleic Acids Res 2004 : Human PCNA could stimulate the human Fen1 extrahelical 3'-flap pocket mutants but not restore their specificity
Wang et al., Proc Natl Acad Sci U S A 2004 : FEN1 activity is stimulated by proliferating cell nuclear antigen ( PCNA ), a toroidal sliding clamp that acts as a platform for DNA replication and repair complexes
Wang et al., J Biol Chem 2005 (Bloom Syndrome...) : Although proliferating cell nuclear antigen stimulates FEN1 , it can not resolve secondary structures