Gene interactions and pathways from curated databases and text-mining

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CDK8 — MED10

Pathways - manually collected, often from reviews:

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

Text-mined interactions from Literome

Lee et al., Mol Cell Biol 1999 : The Mediator complex of Saccharomyces cerevisiae is required for both general and regulated transcription of RNA polymerase II (PolII) and is composed of two stable subcomplexes ( Srb4 and Rgr1 subcomplexes )
Lo et al., Anticancer Res 1999 : CA, CGA and FA could suppress the bacterial NAT activities dose-dependently both in the intact cell and cytosolic fraction analysis ... These results strongly demonstrated that CA, CGA and FA inhibited NAT activities in human gastrointestinal bacteria
Bryan-Lluka et al., Naunyn Schmiedebergs Arch Pharmacol 1999 : Haloperidol, its five metabolites and MPP+ and MPTP all inhibited NAT , DAT and SERT
Spåhr et al., J Biol Chem 2000 : This stimulation was species-specific, because S. pombe Mediator could not stimulate TFIIH purified from S. cerevisiae
Alonso-Gómez et al., Life Sci 2000 : A D2-like receptor is directly involved in the regulation of NAT activity and melatonin release in R. perezi retina
Soros et al., J Virol 2001 : It has previously been reported that Sam68 synergistically stimulates Rev activity ( T. Reddy et al., Nat
Mittler et al., EMBO Rep 2001 : Novel critical role of a human Mediator complex for basal RNA polymerase II transcription
Wang et al., J Biol Chem 2002 : Mediator role of platelet derived growth factor and ERK
Wang et al., J Virol 2002 (Cell Transformation, Viral) : Much of the large E1A bound to Mediator in 293 cells is in a stable complex that includes RNA polymerase II, leading us to suggest that the interaction of E1A-CR3 with Mediator stabilizes the interaction of Mediator with the polymerase
Leineweber et al., Circ Res 2002 (Disease Models, Animal...) : Twenty-one to twenty-eight days after MCT ( group B ), however, not only RV weight ( 316+/-4 versus 148+/-2 mg ) and RV/LV weight ratio ( 0.61+/-0.01 versus 0.3+/-0.01 ) were markedly increased but also plasma noradrenaline ( 645+/-63 versus 278+/-18 pg/mL ) ; now, RV beta-AR density ( 13.4+/-1.3 versus 26.5+/-1.1 fmol/mg protein ), RV NAT density ( 50.9+/-11.3 versus 79.6+/-2.9 fmol/mg protein ), and RV NAT activity ( 65.4+/-7.4 versus 111.8+/-15.9 pmol [ 3H ] -NA/mg tissue slices/15 min ) were significantly decreased and RV-membrane GRK activity ( 100+/-15 versus 67+/-6 [ 32P ] -rhodopsin in cpm ) significantly increased
Acevedo et al., Mol Cell Biol 2003 : We have used a biochemical approach, including an in vitro chromatin assembly and transcription system, to examine the functional role for Mediator in the transcriptional activity of estrogen receptor alpha (ERalpha) with chromatin templates, as well as functional interplay between Mediator and p300/CBP during ERalpha dependent transcription ... Using three different approaches to functionally inactivate Mediator ( immunoneutralization, immunodepletion, and inhibitory polypeptides ), we find that Mediator is required for maximal transcriptional activation by ligand activated ERalpha
Zakharova et al., J Biol Chem 2003 : Furthermore, in a system with purified proteins and naked DNA, STAT1alpha- and STAT1beta dependent transcription is stimulated by the TRAP/Mediator co-activator complex ... Furthermore, in a system with purified proteins and naked DNA, STAT1alpha- and STAT1beta dependent transcription is stimulated by the TRAP/Mediator co-activator complex
Zawilska et al., Acta Neurobiol Exp (Wars) 1992 : It is suggested that Ca2+ may regulate NAT activity indirectly, by affecting the intracellular cyclic AMP content, which is, in turn, critical in the regulation of melatonin biosynthesis
Gupta et al., Journal of neural transmission. General section 1992 : Norepinephrine ( NE ), Delta sleep inducing peptide ( DSIP ), vasoactive intestinal polypeptide (VIP) , adenosine and N-acetyl-asp-glu ( NAAG ) significantly increased NAT activity in rat pineal ... Norepinephrine ( NE ), Delta sleep inducing peptide ( DSIP ), vasoactive intestinal polypeptide (VIP), adenosine and N-acetyl-asp-glu ( NAAG ) significantly increased NAT activity in rat pineal ... DSIP and VIP also increase the stimulatory effect of NE on NAT activity
Marshall et al., Vox Sang 2004 (HIV Infections...) : SS + minipool NAT - p24 compared with SS alone resulted in an incremental cost-effectiveness ratio of 1.5 million per QALY gained ( range in sensitivity analysis 1.0-2.1 million per QALY gained ) in this US analysis
Guidi et al., J Biol Chem 2004 : Mediator , a global transcriptional co-activator, dramatically enhances the phosphorylation of the CTD of RNA pol II by holo-TFIIH in vitro ... Using purified proteins we have determined that the subunits of TFIIK are sufficient for Mediator to enhance Kin28 CTD kinase activity and that Mediator enhances phosphorylation of a glutathione S-transferase-CTD fusion protein, despite the absence of multiple Mediator and/or TFIIH interactions with polymerase ... Using purified proteins we have determined that the subunits of TFIIK are sufficient for Mediator to enhance Kin28 CTD kinase activity and that Mediator enhances phosphorylation of a glutathione S-transferase-CTD fusion protein, despite the absence of multiple Mediator and/or TFIIH interactions with polymerase
Sato et al., Mol Cell 2004 : The Mediator is a multiprotein transcriptional coactivator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factors ... The Mediator is a multiprotein transcriptional coactivator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factors
Caesar et al., J Biol Chem 2004 : The involvement of Nat3p and Tfs1p in PKA signaling was supported by caffeine growth inhibition studies
Huber et al., Environ Mol Mutagen 2004 : K/C decreased hepatic NAT dependent PhIP activation by up to 80 % in a dose dependent manner
Liu et al., J Biol Chem 2004 : However, the effect of Aurora-A on p53 DNA binding and transactivation activity was not affected by phosphorylation of Ser-315, a recently identified Aurora-A phosphorylation site of p53 ( Katayama, H., Sasai, K., Kawai, H., Yuan, Z. M., Bondaruk, J., Suzuki, F., Fujii, S., Arlinghaus, R. B., Czerniak, B. A., and Sen, S. ( 2004 ) Nat
Wang et al., Genetics 2004 : The rgr1 and sin4 constitutive phenotype does not require either the MAL-activator or maltose permease, indicating that Mediator represses MAL basal expression ... The role of the Mediator in MAL gene regulation is discussed
Santanavanich et al., J Pineal Res 2005 : Taken together with our previous data, the results indicate that activation of D1-dopamine receptor in bovine pinealocyte stimulates NAT activity and enhances melatonin level whereas activation of D2-dopamine receptor leads to an inhibitory effect and these stimulatory and inhibitory effects act, in part, via a cAMP dependent transcription mechanism
Pavri et al., Mol Cell 2005 : Importantly, Mediator was inactive ( Cdk8+ ) under basal conditions but was activated ( Cdk8- ) upon induction
Malik et al., Trends Biochem Sci 2005 : Dynamic regulation of pol II transcription by the mammalian Mediator complex
Govind et al., Mol Cell Biol 2005 : We confirm the roles of Mediator and SAGA in TATA binding protein (TBP) recruitment and demonstrate that all four coactivators under study enhance Pol II recruitment or promoter clearance following TBP binding
Takagi et al., J Biol Chem 2006 : Taken together, these findings lead to the suggestion that Mediator is required for basal RNA polymerase II transcription in vivo
Lee et al., In Vivo 2005 (Adenocarcinoma...) : The main objective of this study was to document the effects of 5-MOP on the modulation of NAT activities in the stomach and colon of rats and human stomach and colon tumor cell lines ... 5-MOP more efficiently inhibited NAT activity in human stomach and colon tumor cell lines than in the stomach and colon of rats
Huber et al., Methods Enzymol 2005 (Neoplasms) : Other coffee components such as polyphenols and K/C-free coffee are also capable of increasing GST and partially of inhibiting NAT , although to a somewhat lesser extent
Kim et al., J Biol Chem 2006 : The beta-catenin transactivation domain bound directly to isolated MED12 and intact Mediator both in vitro and in vivo, and Mediator was recruited to Wnt-responsive genes in a beta-catenin dependent manner
Withyachumnarnkul et al., Int J Biochem 1991 : 10. The results indicate that the IFN-gamma induced NAT suppression requires the integrity of the sympathetic nerve terminals and the IFN-gamma induced enhancement of melatonin production is accomplished through its direct action on pinealocytes
Black et al., Mol Cell 2006 : Using purified proteins, we found that the Mediator regulates this assembly process by binding to p300 and TFIID
Withyachumnarnkul et al., J Pineal Res 1990 : IFN-gamma had no effect on either NAT or HIOMT activities in the pineal glands
Zhou et al., Mol Cell Biol 2006 : We propose that activated Gli3 physically targets the MED12 interface within Mediator in order to functionally reverse Mediator dependent suppression of Shh target gene transcription
Jin et al., J Biol Chem 2007 : In contrast, under the same assay conditions, partially purified NAT from rat brain was highly Ca2+ dependent , membrane associated, and specific for the sn-1-acyl group of PC ... These results reveal that RLP-1 can function as a PE N-acyltransferase, catalytically distinguishable from the known Ca2+ dependent NAT
Liu et al., Mol Cell Biol 2008 : STAF65gamma is required for SPT3/STAGA interaction with core Mediator and for MYC recruitment of SPT3, TAF9, and core Mediator components to the TERT promoter but is dispensable for MYC recruitment of TRRAP, GCN5, and p300 and for acetylation of nucleosomes and loading of TFIID and RNA polymerase II on the promoter
Ge et al., Mol Cell Biol 2008 : These results indicate that there is a conditional requirement for MED1/TRAP220 and that a direct interaction between PPARgamma and Mediator through MED1/TRAP220 is not essential either for PPARgamma stimulated adipogenesis or for PPARgamma target gene expression in cultured fibroblasts ... As Mediator is apparently essential for PPARgamma transcriptional activity, our data indicate the presence of alternative mechanisms for Mediator recruitment, possibly through intermediate cofactors or other cofactors that are functionally redundant with MED1/TRAP220
Mine et al., Metabolism 2008 (Disease Models, Animal...) : Furthermore, NAT also up-regulated hepatic expression of the adiponectin receptor AdipoR2 , although there was no effect on the plasma adiponectin level
Kaur et al., Dis Esophagus 2008 (Esophageal Neoplasms) : Similarly, decreased superoxide dismutase activity was observed in tumor tissue in response to NAT
Stehle et al., Neurosci Lett 1991 (Blindness) : Neither this peptide nor AVP alone did not affect NAT activity, but either substance potentiated the norepinephrine induced enhancement of NAT activity
Beltran et al., Genes Dev 2008 (Adenocarcinoma...) : Ectopic overexpression of this NAT in epithelial cells prevents splicing of the Zeb2 5'-UTR, increases the levels of Zeb2 protein, and consequently down-regulates E-cadherin mRNA and protein ... Therefore, the results presented in this article reveal the existence of a NAT capable of activating Zeb2 expression, explain the mechanism involved in this activation, and demonstrate that this NAT regulates E-cadherin expression
Belakavadi et al., Mol Cell Biol 2008 : Here, we report that phosphorylation of MED1 by mitogen activated protein kinase-extracellular signal regulated kinase ( MAPK-ERK ) promotes its association with Mediator
Contreras-Levicoy et al., FEBS J 2008 : Activation of transcription by PC4 was dependent on the Mediator complex and TFIIA, but was independent of TATA binding protein associated factor
Tsutsui et al., Genes Cells 2008 : While the role of CDK8 has been studied extensively, little is known of the role of CDK11 in Mediator
Thiaville et al., Nucleic Acids Res 2008 : It is unclear whether Mediator complex in yeast is necessary for all RNA polymerase II (Pol II) transcription or if it is limited to genes activated by environmental stress
Camp et al., J Neuroendocrinol 1991 : Activation of adenylate cyclase by forskolin or addition of a cyclic AMP analogue increased both melatonin release and NAT activity
Alarma-Estrany et al., J Pineal Res 2009 : 5-MCA-NAT does not act through NQO2 to reduce intraocular pressure in New-Zealand white rabbit ... Altogether, the results led us to conclude that the in vivo effect of the MT(3) ligand 5-MCA-NAT on IOP is not mediated by the enzyme NQO2 , suggesting the existence of another melatonin receptor
Wakamura et al., J Pharmacobiodyn 1990 : Ebselen and NAT06-123 also markedly inhibited nicotinamide adenine dinuclestide phosphate ( NADPH ) oxidase activity, which is responsible for O2- production in intact cells, and in a particulate fraction prepared from TPA stimulated PMNL, whereas PZ-25 inhibited this enzyme weakly and NAT02-761 did not
Suzuki et al., Toxicol Pathol 2010 (Body Weight...) : MeIQx is metabolically activated by CYP1A2 and then N-acetyltransferase (NAT) , findings that suggest that its carcinogenic potential might be enhanced by simultaneous exposure to chemical ( s ) inducing CYP1A2
Carey et al., Cold Spring Harbor protocols 2010 : INTRODUCTION : The Mediator ( Med ) complex plays a key role in promoter-specific activation of transcription by RNA polymerase II (Pol II)
Kunin et al., PloS one 2010 : Mediator of DNA damage checkpoint 1 ( MDC1 ) contributes to high NaCl induced activation of the osmoprotective transcription factor TonEBP/OREBP
Xu et al., EMBO Rep 2011 (Alzheimer Disease) : Mediator , in a MED12 dependent manner, occupies only AICD bound promoter DNA, indicating that the AICD recruits Mediator to activate transcription
Lin et al., Genes Dev 2011 : Studies in depleted extracts showed that the Mediator coactivator complex, which controls PIC assembly, is also necessary for CHD1 recruitment
Pandorf et al., Am J Physiol Regul Integr Comp Physiol 2012 (Hypothyroidism) : A comparative phylogenetic analysis also suggests that bII NAT mediated regulation has been a conserved trait of placental mammals for most of the eutherian evolutionary history
Ang et al., PLoS Biol 2012 : Furthermore, we have found that Mediator controls the galactose induced protein degradation of Gal80 , which places Mediator genetically upstream of the activator Gal4 ... Furthermore, we have found that Mediator controls the galactose induced protein degradation of Gal80, which places Mediator genetically upstream of the activator Gal4
Lewis et al., Clin Exp Allergy 2013 : Mediator release induced by SCF was accompanied by the up-regulation of the activation marker, CD63
Kim et al., J Biol Chem 2013 : Mediator recruitment to heat shock genes requires dual Hsf1 activation domains and mediator tail subunits Med15 and Med16 ... Mediator recruitment to heat shock genes requires dual Hsf1 activation domains and mediator tail subunits Med15 and Med16
Verger et al., Nucleic Acids Res 2013 : We further show that depletion of MED25 disrupts the association of ERM with the Mediator in vitro
Galbraith et al., Cell 2013 (Neoplasms) : HIF1A Employs CDK8-Mediator to Stimulate RNAPII Elongation in Response to Hypoxia ... HIF1A induces binding of CDK8-Mediator and the super elongation complex ( SEC ), containing AFF4 and CDK9, to alleviate RNAPII pausing
Šedý et al., J Immunol 2013 (Inflammation) : CD160 Activation by Herpesvirus Entry Mediator Augments Inflammatory Cytokine Production and Cytolytic Function by NK Cells
Blum et al., Proc Natl Acad Sci U S A 1989 (Chromosome Deletion) : The relationship of a recently isolated cDNA clone, designated rnat, to genetically polymorphic arylamine N-acetyltransferase ( NAT ; acetyl-CoA : arylamine N-acetyltransferase, EC 2.3.1.5 ) of rabbit liver was established by its expression in monkey kidney COS-1 cells : ( i ) cytosols from transfected cultures contained high levels of an Ac-CoA dependent NAT activity, which was kinetically indistinguishable from that observed in cytosols from livers of genetically rapid-acetylator rabbits ; ( ii ) transfected cells also contained an immunoreactive protein, recognized by NAT-specific antibodies, with identical electrophoretic mobility to NAT from rabbit liver
Newman et al., Endocrinology 1985 : Mediator generation was rapid, with a half-time of approximately 45 sec and was insulin dose dependent
Hein et al., J Pharmacol Exp Ther 1986 : Acetyl CoA dependent p-aminobenzoic acid and p-aminosalicylic acid N-acetyltransferase (NAT) activity was determined in peripheral blood and blood cells from homozygous rapid ( RR ) acetylator ( Bio. 87.20 ) and homozygous slow ( rr ) acetylator ( Bio. 82.73/H ) inbred hamsters and in their F1, F2 and backcross progeny
Wainwright et al., J Neurochem 1984 : EGTA or additional Ca2+ had no effect on pineal NAT activity
Voisin et al., J Neurochem 1993 : In indomethacin treated cells, PGs caused a four-fold increase in NAT activity ... As a result, half-maximal stimulation of NAT by PGs was not associated with an increase in cAMP levels ... These results indicate that an increase in cAMP levels may be responsible for the maximal stimulation of NAT evoked by PGs, whereas half-maximal stimulation of NAT by PGs would rely principally on a calcium/calmodulin dependent mechanism
Yuwiler et al., J Neurochem 1995 : Maximal stimulation of rat pineal NAT by PACAP-38 is not increased further significantly by concurrent stimulation with the two related peptides, vasoactive intestinal polypeptide (VIP) and/or peptide N-terminal histidine C-terminal isoleucine ( PHI ) ... Prior stimulation of rat pineal NAT activity with VIP , PHI, or PACAP-38 reduces the magnitude of subsequent stimulation with PACAP-38 or forskolin ... Prior stimulation of rat pineal NAT activity with VIP, PHI, or PACAP-38 reduces the magnitude of subsequent stimulation with PACAP-38 or forskolin ... Prior stimulation of rat pineal NAT activity with VIP, PHI , or PACAP-38 reduces the magnitude of subsequent stimulation with PACAP-38 or forskolin
Molinero et al., Neurosci Lett 1993 (Hypothyroidism) : The effect of vasoactive intestinal peptide (VIP) on thyroxine type II 5'-deiodinase (5'-D) and N-acetyltransferase (NAT) activities were studied using pineal cells of euthyroid and hypothyroid rats ... Both 5'-D and NAT activities were stimulated not only by VIP , but also by isoproterenol, a beta-adrenergic receptor agonist, and forskolin, a potent activator of adenylate cyclase activity
Walter et al., Pharmazie 1996 : NAT was significantly inhibited by CP ( given 5 d before sacrifice ) but not by plC , SLO or CP when given 2 d before sacrifice ... NAT was significantly inhibited by CP ( given 5 d before sacrifice ) but not by plC, SLO or CP when given 2 d before sacrifice
Walter et al., Immunopharmacol Immunotoxicol 1996 : Both IFN gamma and SLO activated NAT to 120 % ( P < 0.05 ) and 135 % ( P < 0.05 ), respectively ... Both IFN gamma and SLO activated NAT to 120 % ( P < 0.05 ) and 135 % ( P < 0.05 ), respectively ... The results suggested that not only the toxin of gram positive streptococcal bacteria SLO, but also the cytokine IFN gamma can stimulate NAT activity in rat hepatic cytosol ... While the enhancing SLO effect on NAT could not be neutralized by the inhibitor of transcription actinomycin D, NAT stimulation by IFN gamma was abolished by actinomycin D and by the inhibitor of translation, cycloheximide ... While the enhancing SLO effect on NAT could not be neutralized by the inhibitor of transcription actinomycin D, NAT stimulation by IFN gamma was abolished by actinomycin D and by the inhibitor of translation, cycloheximide ... Obviously, SLO activated NAT independent of protein synthesis and different from IFN gamma mediated pathways
Foulkes et al., Proc Natl Acad Sci U S A 1996 : In addition, transfection studies show that ICER powerfully represses NAT transcription
Abdel-Rahman et al., Mutat Res 1998 (Chromosome Aberrations...) : Of these alleles, NAT1*10 is responsible for increased NAT1 enzyme levels and is reported to be associated with increased risk for colorectal and bladder cancers
Han et al., Mol Cell Biol 1999 : The multisubunit Mediator complex of Saccharomyces cerevisiae is required for most RNA polymerase II (Pol II) transcription
Liu et al., Cancer Lett 1998 : In sonicate from transiently transfected COS cells, NAT1 increased CYP1A2 catalyzed adduct formation 4-fold while NAT2 increased adduct formation 12-fold