Gene interactions and pathways from curated databases and text-mining
J Pharmacol Sci 2010, PMID: 20484865

Nitric oxide inhibits lipopolysaccharide-induced inducible nitric oxide synthase expression and its own production through the cGMP signaling pathway in murine microglia BV-2 cells.

Yoshioka, Yasuhiro; Takeda, Nobuo; Yamamuro, Akiko; Kasai, Atsushi; Maeda, Sadaaki

The present study examined the effect of the nitric oxide (NO) donor NOC18 on lipopolysaccharide (LPS)-induced NO production to investigate a regulation mechanism of NO production by microglial cells. LPS increased the levels of NO and inducible NO synthase (iNOS) protein in BV-2 murine microglial cells in a concentration-dependent manner. Pretreatment with NOC18 for 24 h concentration-dependently attenuated the LPS-induced iNOS protein expression and NO production. The inhibitory effect of NOC18 on LPS-induced NO production was partially blocked by LY83583, a soluble guanylate cyclase inhibitor. Pretreatment with dibutyryl guanosine-3',5'-cyclic monophosphate (DBcGMP), a cell-permeable cGMP analogue, for 24 h attenuated partially LPS-induced iNOS protein expression and NO production. Furthermore, the effects of LPS on iNOS and NO production were inhibited by the c-Jun N-terminal kinase (JNK) inhibitor SP600125, and LPS-induced phosphorylation of JNK and c-Jun was inhibited by NOC18 and DBcGMP. These results suggest that NO production by microglial cells is controlled by a negative feedback mechanism via the NO/cGMP signaling pathway.

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Text Mining Data

inducible NO synthase (iNOS) → LPS: " LPS increased the levels of NO and inducible NO synthase (iNOS) protein in BV-2 murine microglial cells in a concentration dependent manner "

iNOS → LPS: " Pretreatment with NOC18 for 24 h concentration-dependently attenuated the LPS induced iNOS protein expression and NO production "

iNOS → LPS: " Pretreatment with dibutyryl guanosine-3',5'-cyclic monophosphate ( DBcGMP ), a cell-permeable cGMP analogue, for 24 h attenuated partially LPS induced iNOS protein expression and NO production "

c-Jun ⊣ LPS: " Furthermore, the effects of LPS on iNOS and NO production were inhibited by the c-Jun N-terminal kinase (JNK) inhibitor SP600125, and LPS induced phosphorylation of JNK and c-Jun was inhibited by NOC18 and DBcGMP "

c-Jun ⊣ iNOS: " Furthermore, the effects of LPS on iNOS and NO production were inhibited by the c-Jun N-terminal kinase (JNK) inhibitor SP600125, and LPS induced phosphorylation of JNK and c-Jun was inhibited by NOC18 and DBcGMP "

iNOS — LPS: " Furthermore, the effects of LPS on iNOS and NO production were inhibited by the c-Jun N-terminal kinase (JNK) inhibitor SP600125, and LPS induced phosphorylation of JNK and c-Jun was inhibited by NOC18 and DBcGMP "

Manually curated Databases

No curated data.