Brain Res 2008,
PMID: 18054898
Zaheer, Asgar; Knight, Scott; Zaheer, Ashna; Ahrens, Marcus; Sahu, Shailendra K; Yang, Baoli
In the present study we report that a replication-defective adenovirus construct of GMF cDNA (GMF-V) induced overexpression of GMF protein in neuroblastoma (N18) cells caused cytotoxicity and loss of cell viability. A significant increase in activation of GSK-3beta occurred after infection with GMF-V when compared with mock and lacZ controls. Overexpression of GMF also increased caspase-3 activity, an early marker of apoptosis. Depletion of GMF gene by introducing GMF-specific siRNA (GsiRNA) completely blocked both activation of GSK-3beta and caspase-3 activation whereas a control scrambled siRNA (CsiRNA) had no effect. A cell-permeable peptide inhibitor of GSK-3beta, and lithium completely prevented GMF-dependent activation of caspase-3. These results demonstrate that GSK-3 mediates activation of the death domain caspase by GMF overexpression. We also show that the phosphorylation of GSK-3-dependent site of Tau was a consequence of GMF-overexpression in N18 cells. Taken together our results imply that GMF is involved in the signaling leading to the activation of GSK-3beta and caspase-3 in N18 cells and strongly suggest its involvement in neurodegeneration since GSK-3beta is known to hyperphosphorylate tau which is associated with the neurotoxicity of neurofibrillary tangles in Alzheimer's disease.
Diseases/Pathways annotated by Medline MESH: Neuroblastoma
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Text Mining Data
caspase-3 → GSK-3beta: "
Depletion of GMF gene by introducing GMF-specific siRNA ( GsiRNA ) completely blocked both activation of
GSK-3beta and
caspase-3 activation whereas a control scrambled siRNA ( CsiRNA ) had no effect
"
caspase-3 → GSK-3beta: "
A cell-permeable peptide inhibitor of GSK-3beta , and lithium completely prevented GMF dependent activation of caspase-3
"
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