Biochem Biophys Res Commun 2002,
PMID: 12459189
Yoo, Nam Jin; Park, Won Sang; Kim, Su Young; Reed, John C; Son, Seong Gon; Lee, Jung Young; Lee, Sug Hyung
The production of bioactive interleukin-1beta (IL-1beta), a pro-inflammatory cytokine, is mediated by activated caspase-1. One of the known molecular mechanisms underlying pro-caspase-1 processing and activation involves interaction between the caspase recruit domains (CARDs) of caspase-1 and a serine/threonine kinase RIP2. While the association of Nod1 with both caspase-1 and RIP2 is already known, the consequences of these interactions are poorly understood. Because Nod1 also binds to RIP2, we hypothesized that Nod1 plays a role in pro-caspase-1 activation and IL-1beta processing. We show here that Nod1 binds to both RIP2 and caspase-1 by CARD interactions. Nod1 enhances pro-caspase-1 oligomerization and pro-caspase-1 processing. Nod1 enhances caspase-1-induced IL-1beta secretion, as well as lipopolysaccharide (LPS)-induced IL-1beta secretion in transfected cells. Moreover, HT1080 cells stably transfected with Nod1 showed higher LPS-induced IL-1beta secretion than non-transfected cells, suggesting a role of Nod1 in LPS-induced responses. Our data indicate that Nod1 can regulate IL-1beta secretion, implying that Nod1 may play a role in inflammatory responses to bacterial LPS.
Document information provided by NCBI PubMed
Text Mining Data
interleukin-1beta (IL-1beta) → caspase-1: "
The production of bioactive
interleukin-1beta (IL-1beta) , a pro-inflammatory cytokine, is
mediated by activated
caspase-1
"
pro-caspase-1 — Nod1: "
Because Nod1 also binds to RIP2, we hypothesized that Nod1 plays a role in pro-caspase-1 activation and IL-1beta processing
"
pro-caspase-1 → Nod1: "
Nod1 enhances pro-caspase-1 oligomerization and pro-caspase-1 processing
"
IL-1beta → caspase-1: "
Nod1 enhances caspase-1 induced IL-1beta secretion, as well as lipopolysaccharide (LPS) induced IL-1beta secretion in transfected cells
"
IL-1beta → Nod1: "
Nod1 enhances caspase-1 induced IL-1beta secretion, as well as lipopolysaccharide (LPS) induced IL-1beta secretion in transfected cells
"
IL-1beta → lipopolysaccharide (LPS): "
Nod1 enhances caspase-1 induced IL-1beta secretion, as well as lipopolysaccharide (LPS) induced IL-1beta secretion in transfected cells
"
IL-1beta → LPS: "
Moreover, HT1080 cells stably transfected with Nod1 showed higher LPS induced IL-1beta secretion than non transfected cells, suggesting a role of Nod1 in LPS induced responses
"
IL-1beta → Nod1: "
Our data indicate that Nod1 can regulate IL-1beta secretion, implying that Nod1 may play a role in inflammatory responses to bacterial LPS
"
Manually curated Databases
In total, 3 gene pairs are associated to this article in curated databases